15 results about "Ethanol fermentation" patented technology

A process for converting pomace derived from the production of fruit wine into a nutrient-rich food product, and a product made by said process. The process comprises milling the pomace, carrying out an anaerobic ethanolic fermentation in an anaerobic fermenter by adding yeast and optionally sugars to produce ethanol; carrying out an aerobic acetic acid fermentation in an aerobic fermenter by adding acetic acid bacteria to produce acetic acid, shearing the acetic acid pomace to produce a raw puree; and further processing the raw puree into a puree or powder. The process can be used to produce a product that qualifies for Kosher certification. The nutrient-rich product can be used as an ingredient in foods as a flavour, texture and color enhancer, to mask bitter flavours or off-notes, as a preservative, to fortify processed foods.

A method for monitoring an ethanol fermentation process involves positioning an accelerometer at or above the liquid level of a process stream in a fermentation vessel. The method includes collecting vibration data from the accelerometer during fermentation, analyzing the frequency spectra of the vibration data, and determining the fermentation rate based on the analysis. The system can also incorporate temperature data to improve prediction accuracy. The method can identify amplitude ranges in the frequency spectra, correlate these to carbon dioxide bubble production, and determine parameters such as bubble size, depth of origin, and microorganism location. The system can provide alerts for deviations in fermentation rate and recommend interventions. Additionally, the system can monitor various stages of ethanol production, including liquefaction, oil separation, centrifugation, evaporation, and drying, using multiple accelerometers to determine process parameters such as viscosity, composition, and equipment performance.

To provide a fermentation efficiency improver for a bioethanol fermentation process as an additive that can improve production efficiency with a smaller amount of use. 【Solution means】The fermentation efficiency improver for a bioethanol fermentation process is characterized by containing a polyoxyalkylene compound (A1) represented by formula (1) and / or a polyoxyalkylene compound (A2) represented by formula (2). R 1 {-(AO 1 )s / (EO)m-(AO 2 )n-OR 2}p (1) R 1 {-(AO 3 )t-(AO 1 )s / (EO)m-(AO 2 )n-OR 2}p (2) R 1 is a reaction residue of a polyol having 3 to 12 carbon atoms, AO 1 , AO 2 is an oxyalkylene group having 3 to 4 carbon atoms, AO 3 is an oxyalkylene group having 2 to 4 carbon atoms, EO is an oxyethylene group, R 2 is a hydrogen atom, a methyl group, an acetyl group, s is an integer of 2 to 25, m is an integer of 2 to 40, n is an integer of 1 to 25, t is an integer of 1 to 15, p is an integer of 3 to 8, / represents a random state, and - represents a block state.

The present application relates to the technical field of bioengineering, and particularly relates to a Kluyveromyces marxianus strain and application thereof in fermentation production of ethanol. The present application provides a Kluyveromyces marxianus strain huJ01, and the preservation number of the strain is CGMCC No.22931. The strain can still maintain good growth and ethanol synthesis capacity under stress conditions such as high temperature, high acid, high ethanol and high salt. Under the optimal conditions of inulinase activity, the ethanol yield can reach 2.41%vol after 20% inoculation and static culture at 60 DEG C for 120 h. The strain is inoculated at 4% in a high stress culture medium containing 11.4% ethanol, pH 3.5 and 3.51% NaCl, and is statically cultured at 40 DEG C for 12 h, and the concentration of the bacterial liquid is significantly increased or not significantly decreased. It is shown that the strain still has strong ethanol fermentation performance under extreme environment, and is suitable for special industrial fermentation scenes such as high salt, high acid and high ethanol.

The application discloses application of soluble lignin in the fields of serving as an enhancer of ethanol fermentation and / or improving stress resistance of yeast under fermentation environmental stress conditions, and adds the soluble lignin to an ethanol fermentation system, so that problems such as pH instability, oxidation damage and heavy metal inhibition in the ethanol fermentation can be solved synchronously, yeast survival rate and ethanol yield are significantly improved, process steps are simplified, and cost is reduced, and the application is suitable for industrialized production of ethanol from raw materials such as agricultural and forestry waste, and provides a green and practical technical path for efficient utilization of whole components of lignocellulose. In addition, the soluble lignin can be recycled, and still has relatively high enhancement effect after recycling for multiple times, and has a wide application prospect in the field of bioethanol fermentation.

The utility model discloses a fuel ethanol fermentation reaction device which comprises a fermentation tank body, a liquid adding pipe is fixedly connected to the top end of the fermentation tank body and used for adding ethanol fermentation liquor into the fermentation tank body, a supporting frame is fixedly connected into the fermentation tank body, a fixing seat is fixedly connected to the supporting frame, and a fixing rod is rotationally connected into the fixing seat; the stirring assembly is arranged on the fixing rod, and the stirring assembly is used for enabling water flow to drive stirring blades arranged at the bottom end of the fixing rod; the stirring assembly comprises a receiving disc fixedly connected to the top end of the fixing rod and a downward flowing cavity formed in the fixing rod, an inlet hole communicated with the downward flowing cavity is formed in the receiving disc, and the outer edge of the receiving disc gradually inclines towards the inlet hole. Through the arrangement of the stirring assembly, when liquid enters the fermentation tank body, the liquid firstly falls on the receiving disc, the receiving disc introduces the liquid into the fixing rod, and the fixing rod directly introduces the liquid into the bottom of the fermentation tank body, so that the receiving disc can prevent the liquid from falling from an over-high place and splashing to influence fermentation and the like.

This invention belongs to the field of microbial fermentation technology and relates to a method for increasing ethanol production through mixed fermentation of *Lactobacillus bakerella* and *Schizophyllum commune*. The steps include: inoculating activated *Schizophyllum commune* and activated *Lactobacillus bakerella* into a fermentation medium for fermentation; the ratio of *Schizophyllum commune* to *Lactobacillus bakerella* is 8.0 × 10⁻⁶. 5 CFU / mL: (5.00×10 7 CFU / mL-1.10×10 8 (CFU / mL). This invention can increase ethanol fermentation yield and provides a basis for industrial applications.

The invention relates to the field of bacteriostatic agents, and discloses a lipopeptide-containing composition and application thereof, the composition comprises lipopeptide and cofactors, and the cofactors comprise at least one of nisin, gluconate, a first component and nipagin ester and / or sodium salt thereof; the first component is at least one of dehydroacetic acid, dehydroacetate, sorbic acid, sorbate, propionate and polylysine; on the basis of the total weight of the composition, the content of the nipagin ester and / or the sodium salt is less than or equal to 10wt%. Compared with antibiotics, the lipopeptide-containing composition provided by the invention has the advantages that the antibacterial effect is equivalent or better, and the lipopeptide-containing composition is friendly to animals and environment and non-toxic; the growth of the ethanol fermentation strain (such as saccharomyces cerevisiae) and the production of ethanol are not influenced, and the growth of the ethanol fermentation strain and the production of ethanol can also be promoted in a specific implementation mode.

The present application is a method for predicting the time series of mycelium concentration, ethanol concentration and glucose concentration in the fermentation process of fuel ethanol. The method constructs a fermentation end time prediction model based on a polynomial regression algorithm, and establishes a mathematical relationship between the fermentation end time and the initial conditions. Through the scaling of time scale, the time series of different batch data is transformed to achieve a unified standard end time. The method establishes a mycelium concentration prediction model based on the XGBoost algorithm. The obtained mycelium concentration and growth rate are added as supplementary mechanism knowledge to the prediction model of ethanol and glucose concentration. The method establishes a dynamic parameter model of artificial neural network, which can provide dynamic parameters according to time variables and environmental variables. By integrating it into the previous mycelium concentration, ethanol concentration and glucose concentration prediction model, the time series prediction of mycelium concentration, ethanol concentration and glucose concentration under different initial conditions can be realized.

The present application relates to the field of ethanol fermentation, and particularly relates to a system and method for producing ethanol and feed from wheat. The system comprises a crushing unit, a fiber water washing unit, a pulp mixing unit, a liquefaction unit, a fermentation unit, a distillation unit, a first separation unit, a second separation unit, an anaerobic unit, an evaporation unit and a drying unit. Due to the cellulose water washing process and twice separation, the clear liquid dry matter is reduced, so that the clear liquid back blending ratio can be as high as 50%. Meanwhile, the amount of clear liquid for evaporation is reduced, and the evaporation load is reduced; the device can be stably operated for a long time, the evaporation unit does not need to be separately stopped for cleaning, and full load operation of the device can be ensured. On the basis of producing ethanol and DDGS products and without increasing energy consumption, the present application adds by-products of fiber feed and protein feed, improves the flexibility of products, and improves the production benefit by more than 10%.

The invention relates to the technical field of biotechnology and ethanol fermentation, in particular to a method for improving corn ethanol yield by enhancing corn fiber enzymolysis saccharification. The method comprises the following steps: step 1, liquefying; step 2, saccharification: adjusting the pH value of the liquefied mash to 5 + / -0.2, then adding a saccharifying enzyme and a cellulase system, and saccharifying to obtain saccharified mash; step 3, synchronous saccharification and fermentation: activated yeast is added into the saccharified mash, after saccharification and fermentation, a cellulase system is added again, and then saccharification and fermentation are continued; and step 4, ethanol distillation. According to the method, the enzymolysis efficiency of corn fibers in the whole enzymolysis saccharification and fermentation process is enhanced, the concentration of ethanol produced by fermentation is high, the content of protein in the distillers' grains obtained after distillation solid-liquid separation is higher, and the yield of corn ethanol is increased.

The present application relates to a kind of single-chromosome large fragment superimposed deletion engineering strains of saccharomyces cerevisiae, it is IV number chromosome large fragment superimposed deletion saccharomyces cerevisiae, the chassis of the saccharomyces cerevisiae is wild type saccharomyces cerevisiae strain CEN.PK113-5D;The IV number chromosome large fragment superimposed deletion includes at least two large fragments of large fragment Chr4-1, Chr4-10, Chr4-11 in IV number chromosome of wild type saccharomyces cerevisiae strain CEN.PK113-5D, and the superimposed deletion of large fragment, thereby obtaining the genome simplified engineering strain, its growth phenotype is significantly better than wild type strain;The present application realizes the optimization of saccharomyces cerevisiae growth performance by single-chromosome large fragment combination deletion strategy, and the obtained engineering strain can be widely used in industrial production scenarios such as ethanol fermentation, biosynthetic chemical synthesis, to provide efficient strain resources and technical support for improving microbial fermentation efficiency and reducing production cost.

The invention relates to the field of microorganisms, in particular to a saccharomyces cerevisiae strain with low yield of glycerol and higher alcohols and application of the saccharomyces cerevisiae strain. The bacterial strain keeps the efficient ethanol fermentation capacity, meanwhile, the synthesis amount of glycerol and higher alcohols is remarkably reduced, and the bacterial strain has good application prospects in the industrial fields of bioethanol preparation, baijiu brewing and the like.

The invention belongs to the technical field of cigarette flavors and fragrances, and discloses a preparation method and application of 2-phenethyl alcohol fermentation liquor. The method comprises the following steps: mixing a tobacco material and water according to a solid-liquid ratio of 1: (5-12) (g / mL), extracting at 90-115 DEG C for 20-40 minutes, and carrying out solid-liquid separation to obtain a tobacco leaf extract culture medium; carrying out degerming treatment on the culture medium, and then adding L-phenylalanine to obtain a preference phenylalanine culture medium; and inoculating saccharomycetes capable of synthesizing 2-phenethyl alcohol, and carrying out fermentation culture to obtain 2-phenethyl alcohol fermentation liquor. The invention not only provides a feasible technical scheme for high-efficiency and low-cost biosynthesis of 2-phenethyl alcohol, but also opens up a new way for high-valued utilization of tobacco byproducts and high-efficiency biosynthesis of natural perfume, and has a wide application prospect.

The invention provides a high-efficiency and energy-saving method and system for recovering a byproduct acetic acid from ethanol fermentation, and belongs to the technical field of biochemical engineering and resource recovery, and the method comprises the following steps: carrying out solid-liquid separation on a fermentation liquid to obtain a filtrate and a bacterium-containing liquid, reusing the bacterium-containing liquid for fermentation, carrying out distillation dealcoholization on the filtrate, and collecting an ethanol product and a dealcoholized liquid to obtain the byproduct acetic acid from ethanol fermentation. And adjusting the pH value of the dealcoholization liquid to be neutral, then carrying out electrodialysis to obtain light chamber effluent and thick chamber effluent, reusing the light chamber effluent for fermentation, adding sulfuric acid into the thick chamber effluent for acidification, and then carrying out rectification to obtain an acetic acid product. Through the unique process combination of dealcoholization and adsorption, the problem of interference of ethanol on acetic acid separation is effectively solved; according to the method, ethanol and acetic acid can be recovered at the same time, the maximum utilization of resources is realized, and the economical efficiency of the whole process is improved; compared with a traditional neutralization method, the method avoids the generation of a large amount of solid waste residues, and is environment-friendly; the method is suitable for treating low-concentration acetic acid fermentation liquor, and is low in energy consumption and high in separation efficiency.