26 results about "Ethanol fermentation" patented technology

The invention discloses an application of soluble lignin as a synergist for ethanol fermentation and / or improvement of stress resistance of yeast under a fermentation environment stress condition, the soluble lignin is added into an ethanol fermentation system, and the problems of pH instability, oxidative damage, heavy metal inhibition and the like in ethanol fermentation can be synchronously solved; the survival rate of yeast and the yield of ethanol are remarkably improved, the process steps are simplified, the cost is reduced, the method is suitable for ethanol industrial production of agricultural and forestry wastes and other raw materials, and a green and practical technical path is provided for efficient utilization of all components of lignocellulose. In addition, the soluble lignin disclosed by the invention can also be recycled, can still keep a relatively high synergistic effect after being recycled for multiple times, and has a wide application prospect in the field of bioethanol fermentation.

The invention relates to an ethanolamine saccharification treatment method for corn straw and application, and belongs to the technical field of biomass resource utilization. The method comprises the following steps: crushing corn straws to 20-60 meshes, mixing the crushed corn straws with an ethanol amine aqueous solution with the volume fraction of 10-60% according to a ratio, reacting at 30-80 DEG C for 3-16 hours, carrying out solid-liquid separation after the reaction, washing, and drying to obtain a saccharified product. According to the method, the lignin removal rate and the reducing sugar yield are remarkably increased under the mild condition, the reducing sugar content can reach 875.09 + / -25 mg / g dry straw, and the lignin removal rate is 93.33 + / -2%. In addition, the lignin removal process accords with a three-index superposition kinetic model. The obtained saccharification product is high in cellulose content and good in structural crystallinity, can be used for preparing nano cellulose and biodegradable materials or can be used as an ethanol fermentation substrate, and has a good industrial application prospect.

A process for converting pomace derived from the production of fruit wine into a nutrient-rich food product, and a product made by said process. The process comprises milling the pomace, carrying out an anaerobic ethanolic fermentation in an anaerobic fermenter by adding yeast and optionally sugars to produce ethanol; carrying out an aerobic acetic acid fermentation in an aerobic fermenter by adding acetic acid bacteria to produce acetic acid, shearing the acetic acid pomace to produce a raw puree; and further processing the raw puree into a puree or powder. The process can be used to produce a product that qualifies for Kosher certification. The nutrient-rich product can be used as an ingredient in foods as a flavour, texture and color enhancer, to mask bitter flavours or off-notes, as a preservative, to fortify processed foods.

The present disclosure concerns a recombinant bacterial host cell capable of providing a nitrogen source to a yeast during fermentation to make ethanol. The recombinant bacterial host cell is capable of converting a biomass into ethanol. The recombinant bacterial host cell has at least one first genetic modification. The at least one genetic modifications confers to the recombinant bacterial host cell the ability to increase, when compared to a corresponding control bacterial cell lacking the at least one first genetic modification, the proteolytic activity associated with the recombinant bacterial host cell. The at least one genetic modification also confers the recombinant bacterial host cell the ability to provide a nitrogen source to a yeast capable of converting the biomass into ethanol, wherein the nitrogen source comprises a peptide, an amino acid and / or ammonia.

A method for monitoring an ethanol fermentation process involves positioning an accelerometer at or above the liquid level of a process stream in a fermentation vessel. The method includes collecting vibration data from the accelerometer during fermentation, analyzing the frequency spectra of the vibration data, and determining the fermentation rate based on the analysis. The system can also incorporate temperature data to improve prediction accuracy. The method can identify amplitude ranges in the frequency spectra, correlate these to carbon dioxide bubble production, and determine parameters such as bubble size, depth of origin, and microorganism location. The system can provide alerts for deviations in fermentation rate and recommend interventions. Additionally, the system can monitor various stages of ethanol production, including liquefaction, oil separation, centrifugation, evaporation, and drying, using multiple accelerometers to determine process parameters such as viscosity, composition, and equipment performance.

The invention provides a novel process for continuously fermenting saccharomyces cerevisiae ethanol, and belongs to the technical field of microorganisms. By comparing macroscopic metabolic parameters in the fermentation process under the conditions of three limited nutrient elements (a carbon source, a nitrogen source and a phosphorus source) and four dilution rates (D = 0.05 h <-1 >, 0.1 h <-1 >, 0.15 h <-1 > and 0.2 h <-1 >), research results show that compared with carbon-limited and nitrogen-limited culture, the macroscopic metabolic parameters in the fermentation process are greatly improved; the continuous culture process of the saccharomyces cerevisiae under phosphorus limitation has the advantages of high cell density, high cell activity, high sugar alcohol conversion rate and the like. The invention develops a new process for continuous ethanol fermentation of saccharomyces cerevisiae under phosphorus limitation, realizes efficient production in a continuous ethanol fermentation process, and has a good industrial application prospect.

The invention belongs to the technical field of biogas preparation, and particularly relates to a method for preparing biogas through intermittent aeration coupling ethanol type fermentation and electric field polarization intensification. According to the method, a non-biological methanogenesis composite reflux way is adopted, an alcohol fermentation base material is refluxed to a hydrolysis area, alcohol reacts with free radicals generated by intermittent aeration to generate a methane precursor, and methane generation is promoted; meanwhile, alcohol fermentation is an effective means for constructing novel direct interspecific electron transfer, the thermodynamic bottleneck of traditional acetic acid production and hydrogen nutrition type methane production can be avoided, the mass transfer efficiency and the system stability are improved, and the anaerobic reactor can effectively produce gas at the pH value of 3.5-8; free radicals generated by intermittent aeration and oxidation-reduction oscillation can promote decomposition of a substrate in a hydrolysis area through free radical generation and facultative bacterium growth; the activity of respiratory enzyme is enhanced through the coupling electric field and electric field polarization, the yield of free radicals and alcohol and the metabolism of methanogens are improved, and the yield of biogas is systematically improved.

To provide a fermentation efficiency improver for a bioethanol fermentation process as an additive that can improve production efficiency with a smaller amount of use. 【Solution means】The fermentation efficiency improver for a bioethanol fermentation process is characterized by containing a polyoxyalkylene compound (A1) represented by formula (1) and / or a polyoxyalkylene compound (A2) represented by formula (2). R 1 {-(AO 1 )s / (EO)m-(AO 2 )n-OR 2}p (1) R 1 {-(AO 3 )t-(AO 1 )s / (EO)m-(AO 2 )n-OR 2}p (2) R 1 is a reaction residue of a polyol having 3 to 12 carbon atoms, AO 1 , AO 2 is an oxyalkylene group having 3 to 4 carbon atoms, AO 3 is an oxyalkylene group having 2 to 4 carbon atoms, EO is an oxyethylene group, R 2 is a hydrogen atom, a methyl group, an acetyl group, s is an integer of 2 to 25, m is an integer of 2 to 40, n is an integer of 1 to 25, t is an integer of 1 to 15, p is an integer of 3 to 8, / represents a random state, and - represents a block state.

The application provides a composite membrane combining polytetrafluoroethylene (PTFE) nanoparticles and zirconium oxide (ZrO2) nanocrystals, the surface energy of the composite membrane is effectively reduced, thereby reducing the microbial adhesion in the fermentation broth. The ZrO2 nanocrystals can form small pores, reducing protein plugging and adsorption, and further relieving membrane pollution in the fermentation broth. This double defense mechanism significantly improves the stable flux of the composite membrane in the ethanol fermentation broth, and widens the application prospect of the composite membrane in fermentation broth clarification and biological product recovery.

The present application relates to the technical field of bioengineering, and particularly relates to a Kluyveromyces marxianus strain and application thereof in fermentation production of ethanol. The present application provides a Kluyveromyces marxianus strain huJ01, and the preservation number of the strain is CGMCC No.22931. The strain can still maintain good growth and ethanol synthesis capacity under stress conditions such as high temperature, high acid, high ethanol and high salt. Under the optimal conditions of inulinase activity, the ethanol yield can reach 2.41%vol after 20% inoculation and static culture at 60 DEG C for 120 h. The strain is inoculated at 4% in a high stress culture medium containing 11.4% ethanol, pH 3.5 and 3.51% NaCl, and is statically cultured at 40 DEG C for 12 h, and the concentration of the bacterial liquid is significantly increased or not significantly decreased. It is shown that the strain still has strong ethanol fermentation performance under extreme environment, and is suitable for special industrial fermentation scenes such as high salt, high acid and high ethanol.

The application discloses application of soluble lignin in the fields of serving as an enhancer of ethanol fermentation and / or improving stress resistance of yeast under fermentation environmental stress conditions, and adds the soluble lignin to an ethanol fermentation system, so that problems such as pH instability, oxidation damage and heavy metal inhibition in the ethanol fermentation can be solved synchronously, yeast survival rate and ethanol yield are significantly improved, process steps are simplified, and cost is reduced, and the application is suitable for industrialized production of ethanol from raw materials such as agricultural and forestry waste, and provides a green and practical technical path for efficient utilization of whole components of lignocellulose. In addition, the soluble lignin can be recycled, and still has relatively high enhancement effect after recycling for multiple times, and has a wide application prospect in the field of bioethanol fermentation.

The utility model discloses a fuel ethanol fermentation reaction device which comprises a fermentation tank body, a liquid adding pipe is fixedly connected to the top end of the fermentation tank body and used for adding ethanol fermentation liquor into the fermentation tank body, a supporting frame is fixedly connected into the fermentation tank body, a fixing seat is fixedly connected to the supporting frame, and a fixing rod is rotationally connected into the fixing seat; the stirring assembly is arranged on the fixing rod, and the stirring assembly is used for enabling water flow to drive stirring blades arranged at the bottom end of the fixing rod; the stirring assembly comprises a receiving disc fixedly connected to the top end of the fixing rod and a downward flowing cavity formed in the fixing rod, an inlet hole communicated with the downward flowing cavity is formed in the receiving disc, and the outer edge of the receiving disc gradually inclines towards the inlet hole. Through the arrangement of the stirring assembly, when liquid enters the fermentation tank body, the liquid firstly falls on the receiving disc, the receiving disc introduces the liquid into the fixing rod, and the fixing rod directly introduces the liquid into the bottom of the fermentation tank body, so that the receiving disc can prevent the liquid from falling from an over-high place and splashing to influence fermentation and the like.

The present application belongs to the technical field of biological natural gas preparation, and particularly relates to a method for preparing biological natural gas by coupling intermittent aeration with ethanol fermentation and electric field polarization enhancement. The present application adopts a non-biological methane production composite reflux approach, and refluxes the substrate of alcohol fermentation to a hydrolysis zone. Alcohol reacts with free radicals generated by intermittent aeration to generate methane precursors, thereby promoting methane generation. At the same time, alcohol fermentation is an effective means for constructing a new type of direct interspecies electron transfer, can bypass the thermodynamic bottleneck of traditional acetate production and hydrogenotrophic methanogenesis, and can improve mass transfer efficiency and system stability, so that the anaerobic reactor can effectively produce gas at a pH value of 3.5-8. The free radicals and redox oscillation generated by intermittent aeration can promote the decomposition of the substrate in the hydrolysis zone through free radical generation and growth of facultative bacteria. The present application enhances the activity of respiratory enzymes by coupling an electric field, enhances the yield of free radicals and alcohol, and enhances the metabolism of methanogens, thereby improving the yield of biological natural gas.

This invention belongs to the field of microbial fermentation technology and relates to a method for increasing ethanol production through mixed fermentation of *Lactobacillus bakerella* and *Schizophyllum commune*. The steps include: inoculating activated *Schizophyllum commune* and activated *Lactobacillus bakerella* into a fermentation medium for fermentation; the ratio of *Schizophyllum commune* to *Lactobacillus bakerella* is 8.0 × 10⁻⁶. 5 CFU / mL: (5.00×10 7 CFU / mL-1.10×10 8 (CFU / mL). This invention can increase ethanol fermentation yield and provides a basis for industrial applications.

The invention relates to the field of bacteriostatic agents, and discloses a lipopeptide-containing composition and application thereof, the composition comprises lipopeptide and cofactors, and the cofactors comprise at least one of nisin, gluconate, a first component and nipagin ester and / or sodium salt thereof; the first component is at least one of dehydroacetic acid, dehydroacetate, sorbic acid, sorbate, propionate and polylysine; on the basis of the total weight of the composition, the content of the nipagin ester and / or the sodium salt is less than or equal to 10wt%. Compared with antibiotics, the lipopeptide-containing composition provided by the invention has the advantages that the antibacterial effect is equivalent or better, and the lipopeptide-containing composition is friendly to animals and environment and non-toxic; the growth of the ethanol fermentation strain (such as saccharomyces cerevisiae) and the production of ethanol are not influenced, and the growth of the ethanol fermentation strain and the production of ethanol can also be promoted in a specific implementation mode.

The present application relates to the field of ethanol production, and discloses a method and system for separating fusel oil in an ethanol fermentation process and application thereof, the method comprising: subjecting mature mash to crude distillation in a crude distillation column and a crude auxiliary column to obtain waste mash, crude auxiliary column bottom liquid and crude distillation fraction; subjecting the waste mash to solid-liquid separation to obtain clear liquid; subjecting the clear liquid to evaporation and concentration to obtain evaporation condensate; subjecting the crude distillation fraction to rectification to obtain rectification column fusel and ethanol; subjecting the rectification column fusel to separation to obtain fusel oil and light wine; wherein the separation mode comprises: sequentially mixing the crude auxiliary column bottom liquid and / or evaporation condensate with the rectification column fusel and optional process water, cooling and separating fusel oil to obtain fusel oil and light wine. The method of the present application can improve the separation effect of fusel oil, reduce the content of fusel oil in light wine and industrial wine, and solve or greatly alleviate the problem of fouling in the recovery column.

The present application is a method for predicting the time series of mycelium concentration, ethanol concentration and glucose concentration in the fermentation process of fuel ethanol. The method constructs a fermentation end time prediction model based on a polynomial regression algorithm, and establishes a mathematical relationship between the fermentation end time and the initial conditions. Through the scaling of time scale, the time series of different batch data is transformed to achieve a unified standard end time. The method establishes a mycelium concentration prediction model based on the XGBoost algorithm. The obtained mycelium concentration and growth rate are added as supplementary mechanism knowledge to the prediction model of ethanol and glucose concentration. The method establishes a dynamic parameter model of artificial neural network, which can provide dynamic parameters according to time variables and environmental variables. By integrating it into the previous mycelium concentration, ethanol concentration and glucose concentration prediction model, the time series prediction of mycelium concentration, ethanol concentration and glucose concentration under different initial conditions can be realized.

This invention provides a gastrodia enzyme, its preparation method, and its application. The preparation method of the gastrodia enzyme includes the following steps: washing fresh gastrodia, pulping, enzyme inactivation, cooling, ultrasonic extraction, enzymatic hydrolysis, filtration, homogenization, primary sterilization, inoculation fermentation, degassing, and secondary sterilization. The inoculation fermentation step involves adding an enzyme fermentation agent and baker's yeast for symbiotic fermentation. The preparation method of this invention has a stable process, can ensure a high gastrodia enzyme content, and inhibits the ethanol fermentation pathway, thus preserving the flavor of the gastrodia enzyme to the greatest extent.

The present application relates to the field of ethanol fermentation, and particularly relates to a system and method for producing ethanol and feed from wheat. The system comprises a crushing unit, a fiber water washing unit, a pulp mixing unit, a liquefaction unit, a fermentation unit, a distillation unit, a first separation unit, a second separation unit, an anaerobic unit, an evaporation unit and a drying unit. Due to the cellulose water washing process and twice separation, the clear liquid dry matter is reduced, so that the clear liquid back blending ratio can be as high as 50%. Meanwhile, the amount of clear liquid for evaporation is reduced, and the evaporation load is reduced; the device can be stably operated for a long time, the evaporation unit does not need to be separately stopped for cleaning, and full load operation of the device can be ensured. On the basis of producing ethanol and DDGS products and without increasing energy consumption, the present application adds by-products of fiber feed and protein feed, improves the flexibility of products, and improves the production benefit by more than 10%.

The invention relates to the technical field of biotechnology and ethanol fermentation, in particular to a method for improving corn ethanol yield by enhancing corn fiber enzymolysis saccharification. The method comprises the following steps: step 1, liquefying; step 2, saccharification: adjusting the pH value of the liquefied mash to 5 + / -0.2, then adding a saccharifying enzyme and a cellulase system, and saccharifying to obtain saccharified mash; step 3, synchronous saccharification and fermentation: activated yeast is added into the saccharified mash, after saccharification and fermentation, a cellulase system is added again, and then saccharification and fermentation are continued; and step 4, ethanol distillation. According to the method, the enzymolysis efficiency of corn fibers in the whole enzymolysis saccharification and fermentation process is enhanced, the concentration of ethanol produced by fermentation is high, the content of protein in the distillers' grains obtained after distillation solid-liquid separation is higher, and the yield of corn ethanol is increased.

The invention discloses a method for strengthening directional synthesis of medium-chain fatty acid from kitchen waste, and belongs to the technical field of resource utilization of organic waste. The method comprises the following steps: pretreating the kitchen waste to prepare slurry with the total solid concentration of 10-20%; then respectively carrying out ethanol synthesis and short-chain fatty acid synthesis to obtain ethanol fermentation liquor and SCFAs fermentation liquor; and finally, centrifugally filtering the ethanol fermentation liquor or the ethanol fermentation liquor and the SCFAs fermentation liquor, mixing to form mixed fermentation liquor, inoculating chain extension sludge, adding 0.1-0.1% (v / v) of chloroform, adding a fermentation culture medium and trace elements, and carrying out anaerobic fermentation to generate medium-chain fatty acid. According to the method, only the kitchen waste slurry is used as a raw material, directional fermentation is realized by accurately regulating and controlling parameters of each stage, an electron donor, an electron acceptor and an auxiliary strengthening technology are not needed, and the production cost is reduced. Competitive microorganisms such as methanogens, acetogens and the like can be synchronously inhibited by adding chloroform, chain extension function microorganisms are not influenced, and the yield and purity of MCFAs are effectively improved.

The present application relates to a kind of single-chromosome large fragment superimposed deletion engineering strains of saccharomyces cerevisiae, it is IV number chromosome large fragment superimposed deletion saccharomyces cerevisiae, the chassis of the saccharomyces cerevisiae is wild type saccharomyces cerevisiae strain CEN.PK113-5D;The IV number chromosome large fragment superimposed deletion includes at least two large fragments of large fragment Chr4-1, Chr4-10, Chr4-11 in IV number chromosome of wild type saccharomyces cerevisiae strain CEN.PK113-5D, and the superimposed deletion of large fragment, thereby obtaining the genome simplified engineering strain, its growth phenotype is significantly better than wild type strain;The present application realizes the optimization of saccharomyces cerevisiae growth performance by single-chromosome large fragment combination deletion strategy, and the obtained engineering strain can be widely used in industrial production scenarios such as ethanol fermentation, biosynthetic chemical synthesis, to provide efficient strain resources and technical support for improving microbial fermentation efficiency and reducing production cost.

The invention relates to the field of microorganisms, in particular to a saccharomyces cerevisiae strain with low yield of glycerol and higher alcohols and application of the saccharomyces cerevisiae strain. The bacterial strain keeps the efficient ethanol fermentation capacity, meanwhile, the synthesis amount of glycerol and higher alcohols is remarkably reduced, and the bacterial strain has good application prospects in the industrial fields of bioethanol preparation, baijiu brewing and the like.

The invention belongs to the technical field of cigarette flavors and fragrances, and discloses a preparation method and application of 2-phenethyl alcohol fermentation liquor. The method comprises the following steps: mixing a tobacco material and water according to a solid-liquid ratio of 1: (5-12) (g / mL), extracting at 90-115 DEG C for 20-40 minutes, and carrying out solid-liquid separation to obtain a tobacco leaf extract culture medium; carrying out degerming treatment on the culture medium, and then adding L-phenylalanine to obtain a preference phenylalanine culture medium; and inoculating saccharomycetes capable of synthesizing 2-phenethyl alcohol, and carrying out fermentation culture to obtain 2-phenethyl alcohol fermentation liquor. The invention not only provides a feasible technical scheme for high-efficiency and low-cost biosynthesis of 2-phenethyl alcohol, but also opens up a new way for high-valued utilization of tobacco byproducts and high-efficiency biosynthesis of natural perfume, and has a wide application prospect.

The invention discloses an application of a sodium alginate / lignin composite gel immobilized yeast carrier in improving the ethanol fermentation efficiency under the fermentation environment stress condition, the sodium alginate / lignin composite gel immobilized yeast carrier can resist various stresses at the same time, the survival rate and the fermentation efficiency of yeast are improved, the ethanol concentration of the product is increased, and the yield of the product is increased. The substrate consumption rate is improved. Meanwhile, the sodium alginate / lignin composite gel immobilized yeast carrier is simple in preparation process, can be recycled, can still keep a relatively high synergistic effect after being recycled for multiple times, and has a wide application prospect in the field of industrial production of bioethanol.

The invention provides a high-efficiency and energy-saving method and system for recovering a byproduct acetic acid from ethanol fermentation, and belongs to the technical field of biochemical engineering and resource recovery, and the method comprises the following steps: carrying out solid-liquid separation on a fermentation liquid to obtain a filtrate and a bacterium-containing liquid, reusing the bacterium-containing liquid for fermentation, carrying out distillation dealcoholization on the filtrate, and collecting an ethanol product and a dealcoholized liquid to obtain the byproduct acetic acid from ethanol fermentation. And adjusting the pH value of the dealcoholization liquid to be neutral, then carrying out electrodialysis to obtain light chamber effluent and thick chamber effluent, reusing the light chamber effluent for fermentation, adding sulfuric acid into the thick chamber effluent for acidification, and then carrying out rectification to obtain an acetic acid product. Through the unique process combination of dealcoholization and adsorption, the problem of interference of ethanol on acetic acid separation is effectively solved; according to the method, ethanol and acetic acid can be recovered at the same time, the maximum utilization of resources is realized, and the economical efficiency of the whole process is improved; compared with a traditional neutralization method, the method avoids the generation of a large amount of solid waste residues, and is environment-friendly; the method is suitable for treating low-concentration acetic acid fermentation liquor, and is low in energy consumption and high in separation efficiency.