The application discloses an inhibitor compound ZWT0127 of
receptor-interacting
protein kinase 2 and a preparation method and application thereof, and can effectively solve the problems of preparation and application of the inhibitor compound ZWT0127 in preparation of a
drug for treating acute
lung injury. The method is as follows: N-Boc-4-chloro-5,7,8-trihydro-pyridopyrimidine and 5-amino
benzothiazole are dissolved by using N,N-
dimethylformamide,
pyridine is added, stirring is conducted, and condensation
reflux is conducted;
dilution is conducted, an
ethyl acetate layer is washed,
pyridine is removed, and then the
ethyl acetate layer is washed by using a saturated
sodium chloride solution; the
organic layer is dried by using
anhydrous magnesium sulfate and concentrated to obtain a brown oil-shaped crude product; preliminary purification is conducted,
drying is conducted after concentration,
methanol is used for dissolving, a filter membrane is used for filtering, purification and separation are conducted, a detection
wavelength is used, and a target peak flow fraction of a
retention time is collected;
methanol is recovered by using
pressure reduction and concentration, and freeze-
drying or
vacuum drying is conducted, so that the inhibitor compound ZWT0127 of
receptor-interacting
protein kinase 2 is obtained. The
raw material is rich, the method is easy to operate, the product quality is good, the product has an inhibiting effect on RIPK2
enzyme activity and a treating activity on LPS-induced acute
lung injury.