60results about How to "Overcome toxicity" patented technology

The invention relates to a method for preparing black zirconia ceramics at the low temperature and belongs to the technical field of the preparation of high-temperature structural material. The method respectively uses a homogeneous precipitation method for synthesizing a nanometer coloring agent, and a coprecipitation method for synthesizing nanometer zirconia powder, and adopts Fe-Co-Ni-Mn as a coloring agent. The zirconia powder is not added with any sintering additive, only added with small amount of coloring agent and sintered at the temperature of 1150-1350 DEG C, thus obtaining the black zirconia ceramics with excellent performance and bright color. As the homogeneous precipitation method is adopted for synthesizing coloring agent powder, the monodisperse nanometer particles with uniform powder granules, narrow size distribution and high purity are obtained so that the coloring agent easily colors the particles. As the coprecipitation method is used for synthesizing zirconia powder, nanometer particles with small granularity, big superficial area and high activity are obtained, and can reduce the temperature of the solid-phase reaction, greatly reduce the sintering temperature and solve the problem that the black coloring agent oxide decomposes and volatiles at the high temperature. Simultaneously the method adopts the Fe-Co-Ni-Mn as the coloring agent, thus avoiding the poisonous function of Cr on the human body.

A rechargeable lithium-ion (Li-ion) battery employs a solvent-less, low temperature approach to battery manufacturing that forms charge material from kinetic energy of high velocity particles impelled into an aggregation such that bombardment of the particles against other particles in the aggregation forms a charge conveying structure. High velocity bombardment from a carrier gas nozzle accumulates an active charge material (active material) and metal binder in a layered arrangement for the finished battery. Preparation of the particles, such as by ball milling or freeze drying, arranges particle agglomerations. The particle agglomerations, when impelled against other agglomerations or a current collector, forms a layer of cathodic, anodic or electrolytic battery material. The metallic binder conveys charge for mitigating or eliminating a need for a planar current collector underlying the sprayed layer. The resulting layers are suitable for battery operation, and are manufactured in an absence of any solvent drying or disposal.

The invention discloses a double-targeting polypeptide-antibody-drug conjugate, and a prepared method and antineoplastic application thereof. The double-targeting polypeptide-antibody-drug conjugate structurally includes four components of (A), tumor specificity targeting polypeptide; (B), an antineoplastic drug; (C), a mitochondria target functional group; and (D), hydrazone bonds used for connecting the polypeptide and the antineoplastic drug. The tumor specificity targeting polypeptide is connected with the antineoplastic drug by a connecting arm comprising the hydrazone bonds; the antineoplastic drug is connected with the mitochondria target functional group by chemical bonds; and the tumor specificity targeting polypeptide is suitable for targeting the conjugate to a tumour cell and marker protein LAPTM4B carried on the surface of the tumour cell is used as a specificity target. A molecular target is combined with an organelle target, selective uptake of the drug in the tumour cell can be increased, an acting site of a DOX drug can be transferred form cell nucleus to mitochondria, and therefore the condition that the tumour cell is killed by drug resistance is avoided.

The invention discloses an endotoxin adsorbent using a molecular cluster as a functional group and a preparation method thereof, and belongs to the fields of bioseparation and biomedical engineering. By taking agarose gel as a vector, the endotoxin adsorbent is subjected to epoxy activation to be directly coupled with a molecular sieve frame epsilon-polylysine or polyaspartic acid, then the coupled mixture is ingrafted with micromolecular lysine or lycine by virtue of a condensation reaction to prepare the adsorbent. The adsorbent prepared by the method has higher removal rate to endotoxin inserum of a human, but adsorbs less serum protein. Meanwhile, the preparation process of the adsorbent has the advantages of mild conditions, fewer synthesis steps and relatively low material cost.

The present invention relates to a protein A immunoadsorption material for purifying blood and its preparation method. Said protein A immunoadsorption material is a high-molecular material which is obtained by using agarose gel as carrier matrix, making said agarose gel be reacted with diglycidyl ether coupling reagent to obtain active carrier, then making said active carrier and protein A implement coupling reaction. Said invention can be used for making clinical immunoadsorption therapy.

Prodrugs based on gemcitabine structure shown in formula (I) as well as their synthetic method and application are disclosed in the present invention, wherein the definitions for the groups of a, b, c, d, E, Z and V are described in the specification. By modifying the N⁴ group, the solubility, the bioavailability and the organ specificity of the prodrugs are improved. Therefore, the fast metabolism problem is overcome for the produced prodrugs compounds. Intestinal toxicity induced by gemcitabine is decreased. Thereby, the prodrugs can be delivered by oral administration in clinics and further improve their anti-tumor, anti-cancer, anti-infection and diffusion preventing capability, and can also specifically act on liver or colon. The synthetic method is simple and adapted to industrial production.

The invention provides a complex preservative and application thereof in fruit and vegetable preservation. The preservative is prepared by the following steps: enabling a pururan solution to react with a gamma-polyglutamic acid solution, thereby preparing cross-linked polymer powder; dissolving the cross-linked polymer powder in distilled water, thereby obtaining a cross-linked polymer solution; and enabling the cross-linked polymer solution to react with a copper salt aqueous solution, thereby obtaining the product. The pururan is prepared by fermenting and extracting strong bud aureobasidium, and the gamma-polyglutamic acid is prepared by fermenting and extracting bacillus licheniformis. The complex preservative prepared by the invention has excellent film-forming property and moisture retention property and is extremely small in use amount, is capable of obviously improving the preservation effect of fruits and vegetables, is convenient to use and easy to operate, can be widely applied to perishable fruits and vegetables such as mangos, strawberries, young garlic shoot and chives stem and is suitable for fruit and vegetable preservation in fruit and vegetable producing areas, fruit and vegetable markets, factories/enterprises and families.

The invention discloses a method for preparing a natural high-molecular material by the utilization of rare earth metal ion coordination. Through strong coordination between rare earth metal ion and oxygen atom in carboxyl function group, the rare earth metal ion coordinated natural high-molecular material is prepared by an ion permeation and diffusion method. According to the method, carboxyl-modified graphene oxide is introduced into the natural high-molecular material such that mechanical strength of the material is enhanced and structural regularity of the material also can be boosted. By selecting one ingredient from sodium alginate and xanthan gum as the base material, the material can be endowed with good biocompatibility and biodegradability. The method of the invention is simple and feasible. Reaction condition is mild. The prepared natural high-molecular material has good biocompatibility and strong mechanical properties.

The invention discloses Japanese encephalitis virus like particles as well as a preparation method and an application thereof. The Japanese encephalitis virus like particles are prepared by assembling a PrM/E gene of a Japanese Encephalitis virus (JEV) and a JEVRNA replicor with a nucleotide sequence as shown in SEQ ID NO: 1. The preparation method of the Japanese encephalitis virus like particles comprises the steps of: connecting the JEV PrM/E gene amplified by an RT-PCR (Reverse Transcriptase Polymerase Chain Reaction) to a plasmid pTRE-Tight of a Tet-on advance expression system to form a recombination vector, transfecting a Hela Tet-on advanced cell by using an Xfect method, after screening and identifying through HygB, obtaining a Hela cell line for controllably and stably expressing the PrM/E gene through a limiting dilution process, inducing for expressing a PrM/E protein, and packaging to obtain the conventional virus like particles; and transfecting the Hela cell line by using a JEV replicor RNA vector and packaging to obtain the virus like particles with a capacity of transient infection. The Japanese encephalitis virus like particles can be used for preparing a vaccine or used as a detection agent, has better immunogenicity and reactivity, and is safe and reliable.

The present invention relates to pharmaceutical composition containing a physiologically effective dose of a nanoparticle triterpene glycoside or nanoparticle triterpene complex, wherein said complex is a liposome encapsulated compound, or exosome-encapsulated compound.

The invention provides a gamma-polyglutamic acid-Cu (II) preservative and application thereof to preservation of fruits and vegetables. The preservative is prepared from a gamma-polyglutamic acid solution and a copper salt water solution through reaction; gamma-polyglutamic acid is extracted from fermented bacillus licheniformis. The gamma-polyglutamic acid-Cu (II) preservative provided by the invention can be directly eaten; environment and toxin problems caused by inorganic copper are overcome through complexing of gamma-polyglutamic acid and copper; various pathogenic bacteria in the fruits and the vegetables can be inhibited and killed by the preservative and the preservation effect of the fruits and the vegetables is remarkably improved; the gamma-polyglutamic acid-Cu (II) preservative is convenient to use and simple to operate, and can be widely applied to the preservation of the fruits and the vegetables in fruit and vegetable production areas, fruit and vegetable markets, factories and enterprises, and general families.

The invention discloses a method for collecting earthworms by utilizing sasangua cake solution, aiming to provide a method for collecting earthworms with the characteristics of no residual contamination, environmental friendliness, good sampling results, and stable sampling efficiency. The method comprises the following steps: (a): the preparation of the sasangua cake solution is as follows: sasangua cake powder is added into water in ratio of 1:3 by weight, the mixture is boiled by boiling water for 0.5-2 hours, and then is filtered to obtain sasangua cake filtered fluid, the obtained sasangua cake filtered fluid is added into water in ratio of 1:5-9 by weight to prepare the sasangua cake solution; (b): the sasangua cake solution is evenly sprayed on soil samples, with 5-9 litres of the sasangua cake solution on 0.25 square meters, to collect earthworms climbing out. The method is particularly suitable for collecting earthworms in ecological farms, conservation district, or soil region into which ground water may permeate.

The invention discloses a preparation method of a casein keratinase crosslinked polymer membrane, and belongs to the technical field of leather working. The method comprises the following steps: 1) mixing a casein solution with a keratin solution, and then adjusting the pH value of the mixed solution to 6.5-7.5, stewing to obtain a protein mixed solution; 2) adding glutamine transaminase transferase to the mixed solution according to the adding amount of 10-30U of transferase in each gram of dry matter protein, carrying out crosslinking reaction after fully and evenly mixing, and then inactivating, so as to obtain a mixed hybrid polymer of casein and keratin; 3) adding a film-forming agent to the mixed hybrid polymer of casein and keratin, and then molding, so as to obtain the casein keratinase crosslinked polymer membrane. The casein and the keratin are crosslinked under the catalytic action of the glutamine transaminase transferase, so as to form a covalent crosslinked macromolecule polymer. The crosslinked macromolecule polymer is improved in mechanical property, strong in water-resisting property, and good in biocompatibility, can be completely biodegraded, and has a broad application prospect in the fields of a zoology coating and a biological membrane.

The invention relates to a composition containing a moringa oleifera leave water soluble extract as an effective component and used for treating or preventing cancer, and preparation method for the moringa oleifera leave extract. The composition of the invention has low toxicity for normal cells but excellent toxicity for cancer cells. In addition, the composition is water soluble, so that the composition can be very easily absorbed in the cells, and accordingly the composition has beneficial advantages as oral preparations of an anti-cancer therapeutic agent; and the composition can be applied to development of natural anti-cancer new drug.

The invention relates to a preparation method of a skin-care type disinfection and sterilization hand sanitizer, and belongs to the technical field of hand sanitizers. The preparation method comprises the following steps of firstly, by taking banana peel and lemon peel as fermenting substrates, fermenting by probiotics to obtain enzyme, wherein the banana peel contains banana peel element to inhibit bacteria and fungi breeding, and prevent and treat rhagadia and chilblain, and the enzyme contains rich vitamins, enzyme, secondary metabolites and other nutrition elements to resist aging and bacteria, diminish inflammation, and the like; extracting phosvitin from duck eggs, wherein the phosvitin has excellent moisturizing property and nutrition, and can nourish hand skin; extracting tea saponin from tea oil meal, and mixing, so as to obtain the skin-care type disinfection and sterilization hand sanitizer. The prepared skin-care type disinfection and sterilization hand sanitizer has the advantages that the problems of irritation and toxicity to skin due to use of a sterilizer are solved, and the excellent effect of simultaneously nourishing skin and sterilizing is realized; the toxicity is avoided, the bacteria-inhibiting property is strong, the volatility is good, the stability is good, and the skin-care effect is realized.

The invention relates to a pharmaceutical composition containing oxaliplatin and fluorouracil, and especially relates to an application product of the pharmaceutical composition, wherein the application product comprises an oxaliplatin injection and an injection containing fluorouracil. Administration comprises following steps, the oxaliplatin injection is dissolved and diluted for independent intravenous drop infusion, and then the fluorouracil injection is dissolved and diluted for intravenous drop infusion, wherein the oxaliplatin injection must be applied to drop infusion before the fluorouracil injection.

The invention discloses a solid preparation of levamlodipine or pharmaceutically-acceptable salts thereof, which contains nano-microcapsules of the levamlodipine or the pharmaceutically-acceptable salts thereof, and medicinal auxiliary materials, wherein the nano-microcapsules are prepared from the levamlodipine or the pharmaceutically-acceptable salts thereof and a carrier material according to the weight ratio of 7-12:1; the carrier material is a xanthan gum-silk fibroin-aerosil composition; the ratio of the using amount of xanthan gum to the using amount of silk fibroin to the using amount of aerosil is 1.5-4.5:1:1; and the weight ratio of the levamlodipine or the pharmaceutically-acceptable salts thereof to the medicinal auxiliary materials is 1:1. The solid preparation has a regular and unbroken surface and is stable and not decomposed under the radiation of highlight, has substantially unchanged content, substantially unchanged related substances and a remarkable sustained release function, avoids the burst release of medicaments, solves the problem of potential toxicity of benzene sulfonate, reduces toxic or side effect and improves the compliance of patients.

The embodiments described herein pertain generally to an amalgam electrode, and a producing method of the amalgam electrode, and an electrochemical reduction method of carbon dioxide using the amalgam electrode.

The invention discloses a method for preparing methylmercaptodiafenthiuron. The method comprises the following reaction formula described in the specification, wherein in the formula, a methylation reagent is selected from methyl chloride, methyl bromide, methyl benzenesulfonate, methyl trifluoromethansulfonate, methyl p-toluenesulfonate, dimethyl carbonate, trimethyl phosphate, dimethyl sulfate, diazomethane, methyl trichloroethanimidate, and formaldehyde+formic acid; an alkali is selected from M2CO3, NCO3, MHCO3, MXR1, N(XR1)2, MH, NH2 or NO; M is selected from Li, Na or K; N is selected from Mg or Ca; X is selected from O or S; R1 is selected from H or C1-C6 alkyl. The compound shown in the reaction formula undergoes a reaction with the methylation reagent and the alkali in a proper solvent to prepare the methylmercaptodiafenthiuron. According to the method, the methylation reagent with low price is used, so the product cost is reduced; and the method is mild in reaction condition, simple, convenient and feasible in post-treatment method, economic and effective, and prone to industrialized production.