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Endotoxin adsorbent using molecular cluster as functional group and preparation method thereof

An adsorbent and functional group technology, applied in the fields of bioseparation and biomedical engineering, can solve the problems of difficult preparation of anti-endotoxin drugs and α-polylysine, hidden dangers of polymyxin B toxicity, and unsatisfactory removal effect, etc. , to achieve the effect of overcoming toxicity and instability, good blood compatibility, and stable physical and chemical properties

Active Publication Date: 2012-12-26
CROWN MEDICAL TECH DALIAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Therefore, in order to solve the current research on endotoxin removal (1) the difficulty in preparing anti-endotoxin drugs and α-polylysine, etc., the price is expensive; (2) the potential toxicity of polymyxin B; (3) the traditional The hemoperfusion adsorbent has defects such as poor blood compatibility, low selectivity and unsatisfactory removal effect. The present invention provides a cheap and efficient endotoxin adsorbent and its preparation method, which is expected to be used in the clinical treatment of endotoxemia treat

Method used

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  • Endotoxin adsorbent using molecular cluster as functional group and preparation method thereof
  • Endotoxin adsorbent using molecular cluster as functional group and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Preparation of ε-polylysine coupled with lysine as an adsorbent for molecular cluster functional groups

[0029] (1) Epichlorohydrin activated agarose gel

[0030]Take 10ml of the washed Sepharose CL-6B gel, add it to 10ml of an aqueous solution with a concentration of epichlorohydrin of 1% (v / v) and a concentration of NaOH of 0.4M, shake at 40°C at 170rpm for 2 hours, and use Wash the gel with deionized water until neutral;

[0031] (2) Coupling of ε-polylysine

[0032] Prepare 10ml of ε-polylysine aqueous solution with a concentration of 6% (w / v) and a pH of 11, add the gel after the reaction in the previous step and wash it, and stir it with a paddle at 50°C and 300rpm for 3-4h. The gel was thoroughly washed with deionized water;

[0033] (3) Fixation of terminal functional group lysine

[0034] Add a certain mass of lysine to 10ml of MES buffer with pH 4.8 to make the concentration 1% (w / v), then add the gel after the reaction in the previous step, ad...

Embodiment 2

[0035] Example 2: Preparation of ε-polylysine-coupled betaine as an adsorbent for molecular cluster functional groups

[0036] The preparation method of the endotoxin adsorbent 2 with betaine at the end of ε-polylysine is the same as that in Example 1, except that the lysine in step (3) is replaced with betaine, and the other steps and conditions remain unchanged.

Embodiment 3

[0037] Example 3: Preparation of Adsorbent Using Polyaspartic Acid Coupled to Lysine as Molecular Cluster Functional Group

[0038] The preparation method of the adsorbent 3 of polyaspartic acid coupling lysine as molecular cluster functional group comprises four steps, and the first two steps are the same as (1) and (2) in Example 1, only need (2) The epsilon-polylysine in is changed into polyaspartic acid, and all the other conditions are unchanged, and steps (3) and (4) are as follows:

[0039] (3) Connection of the spacer arm

[0040] Add a certain volume of ethylenediamine to 10ml of MES buffer with a pH of 4.8 to make the concentration 10% (v / v), then add the gel after the reaction in the previous step, adjust the pH to 4.8, and then add 0.2g EDC condensing agent, 37°C, 170rpm shaking reaction for 4h, after the reaction is fully washed with deionized water;

[0041] (4) Fixation of terminal functional group lysine

[0042] Add a certain mass of lysine to 10ml of MES b...

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Abstract

The invention discloses an endotoxin adsorbent using a molecular cluster as a functional group and a preparation method thereof, and belongs to the fields of bioseparation and biomedical engineering. By taking agarose gel as a vector, the endotoxin adsorbent is subjected to epoxy activation to be directly coupled with a molecular sieve frame epsilon-polylysine or polyaspartic acid, then the coupled mixture is ingrafted with micromolecular lysine or lycine by virtue of a condensation reaction to prepare the adsorbent. The adsorbent prepared by the method has higher removal rate to endotoxin in serum of a human, but adsorbs less serum protein. Meanwhile, the preparation process of the adsorbent has the advantages of mild conditions, fewer synthesis steps and relatively low material cost.

Description

technical field [0001] The invention belongs to the fields of biological separation and biomedical engineering, and in particular relates to an endotoxin adsorbent with molecular clusters as functional groups and a preparation method thereof for the treatment of endotoxemia. Background technique [0002] Endotoxin, also known as lipopolysaccharides (Lipopolysaccharides, LPS), is a component of the outer membrane of Gram-negative bacteria. In a normal human body, a certain amount of endotoxin can be removed by the liver so that the endotoxin content in the blood can be kept at a low level. If the liver function is severely damaged or the content of endotoxin exceeds the detoxification ability of the liver, the endotoxin absorbed through the intestine can enter the internal circulation through the liver, forming endotoxemia, and then leading to multiple organ failure and even death. According to reports, at least hundreds of thousands of people suffer from endotoxemia in the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/26B01J20/30A61M1/34A61M1/00
Inventor 贾凌云屈亚辉
Owner CROWN MEDICAL TECH DALIAN CO LTD
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