16 results about "Endotoxin removal" patented technology

The invention relates to the technical field of biomacromolecule separation and purification engineering, and discloses a chromatographic separation method for removing endotoxin in a vaccine preparation, which comprises the following steps: preparing an equilibrium buffer solution containing citrate with specific concentration and L-arginine hydrochloride; providing a mixed mode chromatography medium with hydrophobic and ion exchange complex ligands and balancing with a buffer; according to the method, through the synergistic effect of 8.0-12.0 mmol / L of citrate and 150-250 mmol / L of L-arginine hydrochloride, the endotoxin micelle structure is destroyed and is induced to be dissociated into monomers, and meanwhile, L-arginine is used for inhibiting monomer aggregation, so that the monomers are diffused into inner holes of the medium in a small size to be adsorbed, and the endotoxin micelle structure is separated into the medium. Therefore, the problem of separation caused by size overlapping of endotoxin micelles and antigens is solved, and double breakthrough of deep removal of endotoxin and high recovery rate of antigens is realized.

The invention belongs to the technical field of biology, and provides a pseudomonas aeruginosa endotoxin specific binding polypeptide and application thereof. The amino acid sequence of the polypeptide is shown as SEQ ID No. 1, SEQ ID No. 3 or SEQ ID No. 4 in a sequence table. Experiments prove that the polypeptide has excellent affinity with pseudomonas aeruginosa endotoxin, and compared with an existing anti-endotoxin clinical drug polymyxin B, the polypeptide has obviously better pseudomonas aeruginosa endotoxin detoxification capability. On the basis, the invention further provides application of the polypeptide in removal or separation or analysis of the endotoxin of the pseudomonas aeruginosa and application of the polypeptide in preparation of a detoxification medicine for the endotoxin of the pseudomonas aeruginosa. The polypeptide can be widely applied to the biomedical fields of biological separation, biological detection, disease diagnosis and treatment and the like.

The invention relates to the technical field of hemodialysis, in particular to a preparation method and system for a low-endotoxin hemodialysis concentrated solution, and the method comprises the steps: obtaining a preparation data set, extracting a temperature sequence, a pH value sequence and an endotoxin content index value, and calculating a membrane flux distribution sequence and an adsorbent activity distribution sequence. Performing primary dialysis path optimization to generate a first completely optimized network; identifying a target path node pair according to the target endotoxin content value, and performing secondary path extension to generate a second completely optimized network; and finally identifying a temperature and pH value regulation and control sequence corresponding to the target path node to complete preparation. By optimizing the network, the test workload is remarkably reduced, the endotoxin removal efficiency and the process parameter control precision are improved, and the problems that in the prior art, the endotoxin removal efficiency is low, and the product batch stability is poor are solved.

The present application relates to a method for removing endotoxin from alginate oligosaccharide and alginate oligosaccharide with endotoxin removed, aiming to provide a method for efficiently removing endotoxin from alginate oligosaccharide with simple operation and easy industrialization. The technical points are: insoluble particles are removed by centrifugation, mixed Lewis acid is added for synergistic treatment, the treated solution is acidified to form a precipitate, the precipitate is washed with deionized water, an alkaline solution is added to dissolve the precipitate and filter, the filtrate is adsorbed by an adsorption medium, then alginate oligosaccharide is eluted with an organic solvent, and the alginate oligosaccharide with endotoxin removed is obtained after drying. The present application removes endotoxin from alginate oligosaccharide by using mixed Lewis acid synergistic treatment and adsorption by an adsorbent, no impurities are introduced during the preparation process, the endotoxin content in the obtained alginate oligosaccharide is low, the operation is simple, and the preparation process is suitable for industrial production.

The utility model discloses an endotoxin removing device. The endotoxin removing device comprises a tank body and an endotoxin removing assembly, the endotoxin removal assembly is mounted in the tank body and is used for removing endotoxin in pure steam formed by heating and evaporating raw water; the endotoxin removing assembly comprises a first shell, a second shell and a filter screen; the corresponding end parts of the first shell and the second shell are connected to form a frame structure; the filter screens are installed on the two sides of the frame structure, and the filter screens and the frame structure form a hollow frame structure. The frame structure is filled with a silk screen. According to the device, endotoxin in pure steam can be removed, and the purity of the pure steam is improved.

The invention relates to a method for early-stage removal of endotoxin in plasmid DNA preparation. The method comprises the following steps: (1) carrying out microfiltration on a lysis neutralization solution to obtain a filtrate; (2) concentrating the filtrate to obtain a concentrated solution, exchanging the obtained concentrated solution with purified water or a 10 * TE buffer solution, and adjusting the pH value to 4.4-5.6 with acetic acid to obtain an acidic solution; (3) adding zinc sulfate with the final concentration of 0.5-1M into the acidic solution, and incubating at 10-20 DEG C for 20-60 minutes to obtain an incubation solution; and (4) carrying out microfiltration on the incubation liquid, and carrying out ultrafiltration replacement on the obtained filtrate to remove the precipitate. The early clearing method for endotoxin in plasmid DNA preparation is efficient, economical and safe, endotoxin pollution can be controlled from the source, the proportion and yield of superhelix plasmids are considered, and the quality of plasmid DNA is improved. The early clearing method for endotoxin in plasmid DNA preparation can simplify the downstream purification process, and is especially suitable for large-scale industrial production of high-quality plasmid DNA.

A method for depletion or removal of endotoxins from a known or suspected endotoxin-containing source by virtue of a solid phase extraction material in an essentially aqueous system comprising the steps of—providing a known or suspected endotoxin-containing source, —contacting the known or suspected endotoxin-containing source with a positively charged solid phase material having a surface on which ferric iron is immobilised, wherein the solid phase extraction material has immobilised the ferric iron by (2-aminoethyl)amine (TREN) ligand—incubating the known or suspected endotoxin-containing source for a period of time sufficient to bind endotoxin to the porous solid phase material, —separating the solid phase material from the essentially aqueous system, —optionally isolating the essentially aqueous system freed or depleted from endotoxin.

The application discloses a method for removing endotoxin in pullulan and application thereof, and mainly comprises the following steps: crude pullulan extract is precipitated and washed by an organic solvent to obtain pullulan; after being dissolved, a surfactant is added into the solution to make endotoxin aggregates depolymerize into monomolecular form; then, the solution is filtered through an ultrafiltration membrane to remove endotoxin and residual small molecular impurities and inorganic salts; finally, the polysaccharide solution is precipitated by ethanol, dried and crushed to obtain refined pullulan; the endotoxin removal method adopted by the prepared pullulan has little influence on the molecular weight of the pullulan, the endotoxin content of the obtained pullulan is not higher than 0.05 EU / mg, the prepared pullulan meets the medical standard, has high biological safety, the operation steps are simple, the pullulan is easy to realize large-scale production, and the pullulan has the advantages of rapidness, simplicity, low cost and industrialization; and the technical scheme of the application is favorable to promoting the application of the pullulan in the field of tissue engineering.

The invention relates to the technical field of aquaculture, in particular to a preparation method of a phage compound preparation of Chinese softshell turtles. The method comprises the following steps: S1, respectively separating bacillus cereus, vibrio parahaemolyticus and aeromonas hydrophila from soft-shelled turtle bodies suffering from head shaking disease, nail rot disease and hemorrhagic disease, and mixing to prepare a multi-host bacterial suspension, mixing the multi-host bacterium suspension with an environmental bacteriophage sample, performing enrichment induction, screening out a target bacteriophage, and performing purification culture to obtain a bacteriophage proliferation solution; s2, purifying the bacteriophage proliferation liquid to remove endotoxin to obtain a high-purity bacteriophage solution; and S3, preparing the high-purity bacteriophage liquid and the embedding material into the micro-capsule oral preparation through an electrostatic spraying device. The preparation method comprises the following steps: directionally screening high-cracking bacteriophages aiming at main pathogenic bacteria of soft-shelled turtles, constructing a bacteriophage combination, preparing a targeted microcapsule preparation by combining an electrostatic spraying technology through a constructed composite embedding structure, and cooperating with a bacteriophage synergist, so that spectral antibiosis can be realized, and the stability of the preparation is improved.

The application provides a skull flap endotoxin removal device and method, which comprises a vacuum heating device and a moisture-proof device connected with the vacuum heating device. The vacuum heating device comprises a tank body, a tank cover, a locking assembly and a heating assembly arranged in the tank body. The tank cover is connected with a vacuum device through a pipeline. The tank body and the cover are detachably connected through the locking assembly. The heating assembly is arranged in the tank body and located at a middle position. The heating assembly comprises a support column and a plurality of heating discs arranged on the support column in sequence from top to bottom. Heating wires are arranged in the support column and the heating discs. The moisture-proof device is communicated with the tank body and used for removing residual steam in the tank body after vacuum drying. The application is mainly used for physically removing endotoxin in the skull flap and has the advantages of convenient use and convenient endotoxin removal. Meanwhile, the application can avoid the situation of cooling and moisture return.

The present invention relates to a process for purifying nucleic acids, particularly plasmid DNA, from a liquid sample, which contains endotoxins in addition to nucleic acids. The invention involves a process for purifying nucleic acids from a biological sample containing nucleic acids, preferably plasmid DNA, comprising the following steps: a) providing a liquid sample comprising nucleic acids and endotoxins; b) precipitating the nucleic acids and the endotoxins or binding the nucleic acids and the endotoxins on a solid carrier and thereby separating them from the remaining components of the liquid sample; c) optionally washing the precipitate or solid carrier from step b) at least once to further remove remaining components; d) treating the nucleic acids and endotoxins separated in step b), optionally after washing in step c), using a purifying buffer for at least partial removal of the endotoxin; e) optionally washing the precipitate or the solid carrier in step d) at least once with at least one washing composition, and f) optionally collecting the nucleic acids, optionally by eluting from the solid carrier or dissolving the precipitated nucleic acid; wherein the purifying buffer in step d) comprises an amino compound selected from the group consisting of triethylamine, triethanolamine, 2-[Bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol, 1,3-bis[tris(hydroxymethyl)methvlamino]propane, 2-amino-2-(hydroxymethyl)-propane-1,3-diol, and individual salts thereof, said amino compound having a total concentration in the range of 1.1 to 5.0 mol / L, an alcohol capable of dissolving the amino compound in a total amount of 30 to 70 % by weight based on the total weight of the purifying buffer, and water, wherein the purifying buffer has a pH in the range from 3.0 to 8.0.

A method for purifying a sodium alginate powder from endotoxins and endogenous pyrogens is provided. The sodium alginate powder is thermally treated at a temperature selected based on the viscosity of the sodium alginate powder. A suspension is prepared by adding an organic solvent to the sodium alginate powder, whereafter the suspension is mechanically stirred until homogeneous. The organic solvent comprises a mixture of hexane and isopropyl alcohol in a ratio of 0.5:1-2:1. When particles of the sodium alginate powder are sedimented, a liquid dispersed medium is removed from the suspension. The particles of the sodium alginate powder are dried, and a sodium alginate solution is prepared by dissolving the dried particles in pure water. A silver-impregnated activated carbon powder containing 0.01-0.4 wt % silver is added to the sodium alginate solution. The sodium alginate solution is purified by using ultrafiltration first and then lyophilization or spray drying.

The invention belongs to the technical field of biology, and provides a salmonella typhimurium endotoxin specific binding polypeptide and application thereof. The amino acid sequence of the polypeptide is selected from any one of SEQ ID No.1 to SEQ ID No.5 in a sequence list. Experiments prove that the polypeptide can be specifically bound with the salmonella typhimurium endotoxin, and compared with an existing anti-endotoxin clinical drug polymyxin B, the polypeptide has obviously higher specific binding capacity with the salmonella typhimurium endotoxin and also has obviously better salmonella typhimurium endotoxin detoxification capacity. On the basis, the invention also provides application of the polypeptide in removal, separation or analysis of salmonella typhimurium endotoxin and preparation of salmonella typhimurium endotoxin detoxification drugs. The polypeptide can be applied to the biomedical fields of biological separation, biological detection, disease diagnosis and treatment and the like.

The invention discloses a multifunctional flea salivary protein FS58, a preparation method and application thereof in preparation of drugs for treating psoriasis, and belongs to the technical field of biological medicines. The invention provides a multifunctional protein FS58. The amino acid sequence of the multifunctional protein FS58 is as shown in SEQ ID NO. 2. The invention also provides a preparation method, which comprises the following steps: 1, gene synthesis; 2, plasmid construction and amplification; 3, recombinant expression; carrying out 4Ni < + > column affinity chromatography purification; 5, carrying out enzyme digestion and separation on the FS58 fusion protein; 6, carrying out Superdex 75 gel purification; and 7, removing endotoxin. The invention further provides application of the protein FS58 in preparation of drugs for treating psoriasis. The preparation method provided by the invention provides a way and means for efficiently and massively producing FS58. Meanwhile, the FS58 protein disclosed by the invention has the functions of inhibiting angiogenesis, resisting inflammation and regulating immunity in vivo and in vitro, and a new way and means are provided for treating psoriasis.

The present invention relates to a process for purifying nucleic acids, particularly plasmid DNA, from a liquid sample, which contains endotoxins in addition to nucleic acids. The invention involves a process for purifying nucleic acids from a biological sample containing nucleic acids, preferably plasmid DNA, comprising the following steps: a) providing a liquid sample comprising nucleic acids and endotoxins; b) precipitating the nucleic acids and the endotoxins or binding the nucleic acids and the endotoxins on a solid carrier and thereby separating them from the remaining components of the liquid sample; c) washing the precipitate or solid carrier from step b) at least once with a washing buffer comprising an alcohol to further remove remaining components; d) treating the nucleic acids and endotoxins separated in step b), and washed in step c), using a purifying buffer for at least partial removal of the endotoxin; e) optionally washing the precipitate or the solid carrier in step d) at least once with at least one washing composition, and f) optionally collecting the nucleic acids, optionally by eluting from the solid carrier or dissolving the precipitated nucleic acid; wherein the purifying buffer in step d) comprises an amino compound selected from the group consisting of triethylamine, triethanolamine, 2-[Bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol, 1,3-bis[tris(hydroxymethyl)methvlamino]propane, 2-amino-2-(hydroxymethyl)-propane-1,3-diol, and individual salts thereof, an alcohol capable of dissolving the amino compound in a total amount of 30 to 70 % by weight based on the total weight of the purifying buffer, and water, wherein the purifying buffer has a pH in the range from 3.0 to 8.0.

The invention discloses a preparation method of a hypoallergenic acellular matrix implant, and belongs to the technical field of biomedical materials. The preparation method comprises the following steps: swelling animal soft tissues in an alkaline solution for a short time, quickly freezing and crushing; placing the crushed sample in a hypertonic-hypotonic solution system, and performing efficient and mild decellularization treatment through circulating oscillation; then, endotoxin and other sensitizers are specifically removed by adopting a chromatography technology in sequence, denatured protein is washed by utilizing a salt solution, and finally, purification is performed through ultrafiltration or dialysis. According to the method, cell debris, nucleic acid and endotoxin can be deeply removed through multi-step synergy and combination of hypertonic-hypotonic circulating decellularization and chromatographic purification, the immunogenicity of an implant is remarkably reduced, and the natural three-dimensional structure and biological activity of an extracellular matrix are reserved to the maximum extent. The implant prepared by the method has the characteristics of low DNA residual quantity, extremely low endotoxin content and excellent biocompatibility, and is suitable for soft tissue repair and regeneration.