104 results about "Animals vaccines" patented technology

The invention belongs to the field of animal vaccines, and particularly relates to a multi-epitope fusion protein aiming at multi-serotype laisseria parasuis antigen and application. The applicant carries out proteomics analysis on four clinical isolated strains of serotypes 4, 5 and 13 of the parasuis leather serotype 4, 5 and 13 with high toxicity and good immunogenicity, and eight antigen proteins are screened out of the four clinical isolated strains of the serotypes 4, 5 and 13 of the parasuis leather serotype 4, 5 and 13. And further analyzing the screened eight antigen proteins to finally obtain the antigen multi-epitope fusion protein with immunogenicity capable of recognizing three serotypes. When the fusion protein is used as a vaccine, the protection rate of clinically separated piglets infected with a serum type 4 strain HB04, a serum type 5 strain SJZ05 and a serum type 13 strain GD20 reaches 100%.

The invention discloses an mRNA (messenger ribonucleic acid) transcription skeleton vector and application thereof in preparation of a self-amplification type mRNA vaccine, and belongs to the technical field of veterinary biological products. According to the invention, alphavirus non-structural protein regions NSP2 and NSP3 of a self-amplification type mRNA skeleton are modified, a polyA tail is optimized, and a novel self-amplification type mRNA skeleton is constructed; the self-amplification type mRNA vaccine can improve the antigen expression efficiency, reduce the toxicity of non-structural protein to cells and improve the encapsulation efficiency and delivery efficiency by combining with the optimization of the components and proportion of a lipid nano delivery system, and meanwhile, the self-amplification type mRNA vaccine is wide in applicable pathogen antigen range and can be popularized to more animal epidemic diseases. The characteristics of high-efficiency expression, low-toxicity delivery and single-dose immunization are expected to renovate the immunization strategy of the existing animal vaccine, the epidemic prevention cost of the breeding industry is reduced, and the method has a wide industrial application prospect.

The invention relates to the technical field of animal vaccines, and discloses an animal recombinant castration vaccine and a preparation method and application thereof, the animal recombinant castration vaccine comprises an animal castration vaccine antigen and an adjuvant, and the content of the animal castration vaccine antigen in the vaccine is 0.5-1 mg / mL. A gel type vaccine is prepared by adopting an animal castration vaccine antigen with an amino acid sequence as shown in SEQ ID No.2-4, and testis growth and development of rats are obviously inhibited after the rats are immunized. The animal castration vaccine antigen is also used for preparing vaccines in three dosage forms, namely an emulsion type vaccine, an aluminum salt type vaccine and a gel type vaccine, after the three vaccines are used for immunizing rats, the anti-GnRH antibody titer in the rats is obviously increased, the testosterone concentration is reduced (Plt; 0.05), and the testis growth and development are inhibited. The three dosage forms of vaccines all have good immune castration effect, and can be applied to castration or estrus inhibition of pets and economic animals in the future.

The invention provides a water-in-oil adjuvant for animal vaccines and a preparation method and application thereof, and relates to the technical field of biology, the water-in-oil adjuvant comprises 73%-75% of oil for injection, 20%-23% of an emulsifier and 2%-7% of a stabilizer; the emulsifying agent is prepared from 4.5 percent to 5 percent of polyoxyethylene fatty alcohol ether, 3.0 percent to 3.5 percent of glycerol monoisostearate, 7.0 percent to 8.0 percent of span 80, 4.5 percent to 5 percent of Tween 80 and 1 percent to 1.5 percent of vitamin E; the vaccine prepared from the water-in-oil adjuvant has the advantages that the viscosity is reduced, and the injection is easy. The technical problems that in the prior art, a vaccine prepared from an oil-in-water adjuvant is large in viscosity, so that resistance is large during injection, the occurrence rate of side reactions is high, an injection part swells, and granuloma is formed are solved.

The invention provides an animal vaccination risk prediction method and system based on a cross-modal large model, and relates to the technical field of vaccination risk prediction. Comprising a multi-modal data acquisition and integration module, a cross-modal feature extraction and semantic alignment module, a pre-training model construction and transfer learning module, a risk prediction and decision support module and a model verification and iterative optimization module. Misjudgment caused by same surface symptoms can be effectively avoided, in animal epidemic disease diagnosis, similar symptoms may be caused by different viruses, a traditional method is easily interfered by surface phenomena, and the optimized method can be used for diagnosis of animal epidemic diseases by constructing a causal diagram, analyzing real causes of symptoms and stripping confounding factors such as environment and physiological status. Through the means, the model can more accurately distinguish virus types, the misjudgment risk is remarkably reduced, and a reliable basis is provided for a subsequent vaccination strategy.

The invention relates to the technical field of animal vaccines, in particular to a recombinant porcine epidemic diarrhea virus S1 protein and transferrin fusion protein as well as a preparation method and application thereof. According to the present invention, the SpyTag sequence is inserted into the S1 gene sequence, the eukaryotic cell HEK293 is adopted to express the protein, such that the obtained recombinant S1 protein has sufficient glycosylation modification, and when the recombinant S1 protein is mixed with the recombinant transferrin containing the SpyCatcher sequence, the K31 on the SpyTag and the N117 on the SpyCatcher are subjected to spontaneous condensation to form the isomeric peptide so as to display the antigen on the transferrin 24 polymer surface; the technical system not only maintains the natural conformation of the virus antigen protein, but also has the advantage of stimulating the innate and adaptive immune response functions of the host, and has a better protection effect on the host.

The invention discloses a veterinary vaccine composite adjuvant as well as a preparation method and application thereof. Active ingredients in the vaccine composite adjuvant are a manganese-containing compound and a Mincle agonist. According to the veterinary vaccine composite adjuvant disclosed by the invention, Mn and TDB are combined, so that the immunological enhancement effect of the TDB adjuvant is achieved. The composite adjuvant can induce a strong immune response reaction, significantly improves the immune effect, and is free of residual damage in use. Compared with the H7N9 vaccine prepared by singly using DDA-TDB, the H7N9 vaccine prepared by mixing the H7N9 subtype avian influenza inactivated virus as the antigen with the composite adjuvant can enhance humoral immunity and cellular immune response of a body, reduce virus load and improve protection level, and provides a product and technical support for production of veterinary vaccines.

The invention relates to the technical field of animal vaccine adjuvants, and discloses a composite biphasic adjuvant for a swine foot-and-mouth disease animal vaccine as well as a preparation method and application of the composite biphasic adjuvant, and the composite biphasic adjuvant comprises the following components in parts by weight: 50-65 parts of squalene; 0.1 to 0.5 part of phytosterol; 0.05 to 0.2 part of an antioxidant; 1 to 3 parts of chitosan oligosaccharide; 0.5 to 1 part of trehalose; 3-7 parts of an emulsifier; the deionized water is supplemented to 100 parts, and the antioxidant comprises 2-4 parts of a segmented copolymer PluronicF68; 0.5 to 1.5 parts of Tween 80; the antioxidant comprises the following components in parts by weight: 0.03 to 0.1 part of vitamin E (alpha-tocopherol); and 0.02 to 0.1 part of ascorbyl palmitate. By optimizing the squalene oil phase, the dual-antioxidant system and the synergistic emulsifier formula, the technical effects of high emulsion stability, high antigen delivery efficiency and remarkably enhanced immune response are achieved.

This utility model relates to the field of medical equipment technology, and in particular to an animal vaccine injection booster device, comprising a first air chamber, a second air chamber, a piston assembly, and a gun body; the gun body has a first air chamber and a second air chamber, the first air chamber having a gas source inlet, a gas outlet, and a gas delivery outlet, the gas delivery outlet communicating with the second air chamber, and a control valve also being provided in the first air chamber; when the trigger of the gun body presses the control valve, the control valve blocks the connection between the gas delivery outlet and the gas outlet; when the trigger of the gun body is released, the control valve blocks the connection between the gas delivery outlet and the gas source inlet; the second air chamber has a piston assembly, the piston assembly including a piston and a firing pin, the firing pin being disposed on the piston, and an elastic element being provided between the piston and the firing pin output end of the second air chamber; the trigger is disposed on the grip part of the gun body, the grip part having a grip groove on the side near the trigger, and a thumb groove on the top side of the grip part away from the trigger. This utility model is easy to operate and has a good grip feel.

The invention relates to a porcine bacterial polysaccharide-viral protein conjugate vaccine as well as a preparation method and application thereof, and belongs to the technical field of animal vaccines. The vaccine takes porcine bacterial polysaccharide and virus protein as raw materials, and a polysaccharide-virus protein conjugate is formed through coupling of a biotin-avidin system; wherein the virus protein is selected from one of classical swine fever virus E2 protein, porcine circovirus type 2 Cap protein, porcine pseudorabies virus gD protein and the like, and the porcine bacterial polysaccharide is selected from one of streptococcus suis capsular polysaccharide, porcine pasteurella multocida capsular polysaccharide and the like. The vaccine can simultaneously induce an organism to generate a high-level IgG antibody aiming at porcine bacterium capsular polysaccharide and virus protein, so that synergistic immune protection on porcine bacterium and virus infection is realized, and a broad-spectrum cross immune effect is achieved.

The application provides a preparation method of polysaccharide vaccine diluent and application to chicken newcastle disease vaccine, and belongs to the technical field of biopharmacy.The application provides a south five canes polysaccharide vaccine diluent and a preparation method thereof, and solves the problems of low uniformity of antibody titer, stress reaction and allergic reaction of the body after inoculation, and influence on the immunization effect of the vaccine caused by the physiological saline or the phosphate buffer solution diluent for diluting the vaccine.The south five canes polysaccharide vaccine diluent can be applied to animal vaccines, including chicken newcastle disease vaccine, and solves the problem that there is no polysaccharide diluent for the chicken newcastle disease vaccine in the prior art.

1. Name of the product in this design: Animal vaccination station (for veterinary use). 2. Purpose of this design: For vaccinating animals. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The application relates to the technical field of biological medicine, and particularly discloses CpG oligodeoxynucleotides and application thereof. The CpG oligodeoxynucleotide has a sequence as shown in any one of SEQ ID NO. 3-6, 9, and preferably has a sequence as shown in SEQ ID NO. 3-6. The CpG oligodeoxynucleotide can effectively activate the immune system of a human body or an animal, has the functions of preventing and treating pathogenic microorganism infection in a broad spectrum, has the function of improving the immunogenicity of a common vaccine, and can be applied as a human or animal vaccine adjuvant.

The invention discloses a bovine virus diarrhea live vaccine based on suspension culture and a preparation method thereof, and relates to the field of animal vaccines. The bovine viral diarrhea virus vaccine is a freeze-dried vaccine, and each vaccine contains a bovine viral diarrhea virus BVDV attenuated strain antigen and a freeze-drying protective agent; wherein the virus content of the antigen of the BVDV attenuated strain is 105-107 CCID50 / piece, and the BVDV attenuated strain is a cytopathic effect type attenuated strain suitable for suspension culture; the freeze-drying protective agent comprises trehalose, cane sugar, gelatin hydrolysate, sodium glutamate and polyvinylpyrrolidone. The suspension culture technology is adopted, large-scale, efficient and stable production of BVDV antigens is achieved, an optimized freeze-drying formula is combined, the obtained vaccine is high in virus titer, good in immunogenicity, high in stability, good in safety, advanced in production process, controllable in cost and suitable for industrial large-scale production.

The utility model discloses an animal vaccine culture medium wastewater treatment pond capable of treating waste gas, which comprises a treatment pond body, a water inlet and a water outlet, the top end of one side of the treatment pond body is provided with the water inlet, the bottom end of one side of the treatment pond body is provided with the water outlet, and the other side of the treatment pond body is connected with a waste gas treatment box in a welding way. According to the animal vaccine culture medium waste gas treatment device, adsorption force is generated in the waste gas treatment box by starting the air blower, is guided into the adsorption head under the action of the fixed pipe and the adsorption pipe, and is discharged through the adsorption head, so that waste gas generated by animal vaccine culture medium waste gas treatment is conveniently adsorbed; adsorbed waste gas is guided into the waste gas treatment box, the waste gas is filtered and purified under the action of an adsorption plate and a filter plate, and the practical environmental protection performance of the waste gas treatment device is improved through the operation, so that the influence of the waste gas on the surrounding environment and workers is reduced.

The application discloses a production method and a production system of white oil for animal vaccines, and the production method is characterized in that: firstly, raw materials are cut and separated to obtain light components and heavy components; the heavy components enter an adsorption-isomerization reaction unit to be in contact with a catalyst, and after treatment, a material flow A is obtained; when the refractive index of the material flow A is higher than that of the heavy components, the heavy components stop entering the adsorption-isomerization reaction unit; hydrogen is introduced into the adsorption-isomerization reaction unit to generate an isomerization condensation reaction, and the reaction product is further subjected to a hydrogenation refining reaction; after the reaction effluent is separated, a gas phase product, light white oil and white oil for animal vaccines are obtained. According to the method, non-ideal components are separated in advance, so that the isomerization condensation reaction is only performed on raw materials rich in ideal components, hydrogenation refining and isomerization condensation processing loads are reduced, hydrogen consumption in the process is reduced, and the economic efficiency of the device is improved.

The invention discloses a method for removing endotoxin in chicken infectious bursal disease virus VP2 protein. The method comprises the following steps: firstly, crushing a VP2 protein expression strain; adding calcium chloride into the treated VP2 protein, and standing; returning the temperature, adding sodium carbonate into the VP2 protein subjected to temperature returning, and standing; and returning the temperature of the VP2 protein added with the sodium carbonate, taking supernate, adding calcium chloride into the supernate, and standing. By setting the adding concentration of calcium chloride, the adding concentration of sodium carbonate and the treatment sequence and times of calcium chloride and sodium carbonate, the endotoxin in the VP2 protein is efficiently and rapidly removed by using calcium chloride and sodium carbonate. By adopting the method disclosed by the invention, the endotoxin removal rate reaches 99.91%, the agar diffusion titer is improved compared with that before treatment, and the animal vaccine preparation standard is met. The method disclosed by the invention is short in treatment period, large in treatment capacity, low in removal cost and suitable for industrial mass production.

The invention discloses a rapid determination method of virus content, and belongs to the field of animal vaccine quality detection. The specific process comprises the following steps: (1) preparing virus antiserum; (2) diluting a to-be-detected virus solution, and culturing for 22-24 hours; (3) based on the prepared virus antiserum, determining the FAID50 of the virus to be detected, and then determining the TCID50 of the virus to be detected; (4) judging whether the content of the virus to be detected is qualified or not according to the absolute values A of the FAID50 and the TCID50 and the p values of the FAID50 and the TCID50; if the value A is less than or equal to 0.2 and the value p is more than or equal to 0.8, judging that the content of the virus to be detected is qualified, otherwise, judging that the virus is unqualified. The constructed method is low in detection cost and short in detection period, the whole detection period is 28 hours, the problem that the virus content is reduced due to the fact that the preservation time of a semi-finished product is prolonged is solved, and the content of a finished product is increased. In addition, when the virus content is detected, a virus standard substance does not need to be used, and the experiment cost is greatly reduced.

The present invention belongs to the field of animal vaccines, and specifically relates to a multi-epitope fusion protein of Gramseria parasuis antigens targeting multiple serotypes and its application. The applicant conducted proteomic analysis on four clinical isolates of Gramseria parasuis serotypes 4, 5, and 13 with high virulence and good immunogenicity, and screened out 8 antigenic proteins. The 8 screened antigenic proteins were further analyzed, and finally an immunogenic multi-epitope fusion protein of antigens that can recognize the three serotypes was obtained. As a vaccine, the fusion protein achieved a 100% protection rate against piglets infected with the clinically isolated serotype 4 strain HB04, the serotype 5 strain SJZ05, and the serotype 13 strain GD20.

The application belongs to the technical field of animal vaccines, and particularly relates to an O-A-Dex-Ap for African swine fever immunization. Based on the good biocompatibility of Dextran, the application uses the Dextran as a nanoparticle to load a coupled CTL epitope peptide. Preliminary experimental results show that, compared with the epitope peptide alone, the O-A-Dex-Ap after loading and coupling can more effectively promote antigen presentation and activate more persistent CTL immune response, and has good application potential. Based on the results, a good technical foundation can be laid for subsequent preparation of African swine fever and other disease vaccines.

The invention discloses application of CpG ODN in preparation of a medicine for regulating animal immune cell activity, and relates to the technical field of animal vaccines. In the invention, the regulation of the animal immune cell activity is to promote the immune response reaction of immune cells; the step of promoting the immune response reaction of the immune cells comprises the step of promoting the immune cells to generate interferon. The CpG ODN provided by the invention can well and specifically stimulate immune cells of animals, especially pigs to release cytokines and promote the animals to generate rapid and strong immune response to immunogens; when being applied to an animal vaccine, the vaccine can stimulate animals to generate a higher antibody level in a shorter time, and can maintain the high-level antibody for a long time, and the immunogenicity of the vaccine is remarkably improved.

The present invention provides an aerosol protective agent for a porcine pseudorabies live vaccine, and relates to the field of animal vaccines. The aerosol protective agent is an aqueous solution containing the following ingredients: 3-24 g / L trehalose, 3-24 g / L L-arginine, 3-24 g / L proline and 1-10% glycerol, with a pH value of 7.0-7.8. The present invention also provides a method for preparing a porcine pseudorabies live vaccine aerosol, comprising: preparing a solution of a commercially available porcine pseudorabies live vaccine freeze-dried product with an aerosol protective agent, and preparing the aerosol live vaccine by ultrasonic atomization; the aerodynamic mass median particle size of the aerosol live vaccine is 1-10 μm, and after being retained in the air for 30 minutes, it still has a high titer, a high immune protection rate, simple preparation, and low cost.

The invention discloses serum for fermentation of haemophilus parasuis as well as a preparation method and application thereof, and belongs to the technical field of animal vaccine production. The customized serum comprises the following components: newborn calf serum, sodium glutamate, a plant extract, NAD (Nicotinamide Adenine Dinucleotide), MgSO4, glycerol and glycine. All components of the customized serum do not contain porcine exogenous viruses, and the customized serum is matched with a TSB culture medium for use, so that the fermentation time of haemophilus parasuis is shortened, and the antigen density is improved. According to the method disclosed by the invention, the safety is ensured, the problems that the traditional haemophilus parasuis fermentation only uses TSB, NAD and serum, the time is long, and the antigen density is low are solved, and all components needing to be additionally added are added into the customized serum, so that the culture medium preparation process before fermentation is simplified.

The invention belongs to the field of animal vaccines, and discloses an outer membrane protein antigen composition aiming at multiple serotypes of laisseria parasuis and application of the outer membrane protein antigen composition. The applicant carries out proteomics analysis on 4-type, 5-type and 13-type clinical isolated strains of serotypes of laisseria parasuis with high toxicity and good immunogenicity, eight vaccine antigen proteins are screened out, and further screening finds that the protection effect of the combination of the five antigen proteins Pal, Aida, EspP2, LppA and FLK6204400 on weaned piglets is better than that of the combination of the eight proteins. And then the proportions of the antigen components are explored, so that the protective force of the obtained raisseria parasuis outer membrane protein antigen composition with the optimal proportion on raisseria parasuis serotype 4, serotype 5 and serotype 13 reaches 100%.

The present application relates to the technical field of biological medicine, in particular to a kind of mRNA vaccine for obtaining bovine nodular skin disease virus neutralizing antibody and application thereof.The mRNA vaccine, characterized in that, contain A27L antigen selected fragment, or L1R antigen selected fragment.The mRNA vaccine provided by the present application successfully induced effective immune response in mice and cattle, detected high level of serum neutralization titer, and the effect is better than goatpox live vaccine (CVCC AV41) vaccine, and the antibody induced has no antibody-dependent enhancement effect.This breakthrough fills the blank in the current LSDV vaccine research and development field, provides a new solution for the production and research and development of animal vaccine, and has very high commercial value and broad application prospect.

The present invention relates to the field of animal vaccine injection. The present invention relates to a beak trimming injection device. Among them, one scheme of the beak trimming injection device includes a main body, on which a fixing component, a beak trimming component and a groove for accommodating an animal are provided, the beak trimming component is located at the bottom of the groove, and the fixing component fixes the head of the animal in the groove; an injection component, the injection component can be close to or away from the main body, and the injection component can inject into the squeezed neck skin of the animal. In an embodiment of the present invention, the injection component can rotate relative to the main body to accommodate poultry of different sizes, so that the poultry is not easily injured during the injection process.

The present invention relates to the field of animal vaccine injection. The present invention relates to a beak trimming injection device. Among them, one scheme of the beak trimming injection device includes a main body, the main body is provided with a groove, and the bottom of the groove is provided with a beak trimming assembly for beak trimming; a fixing assembly, including a first fixing part and a second fixing part, the first fixing part is used to press the head of the animal into the groove, and the second fixing part is used to squeeze the neck skin of the animal; an injection assembly, and the injection assembly can inject into the squeezed neck skin. Compared with the traditional injection device, the present device clamps the back of the neck of the poultry so that the liquid injected into the cavity between the skin and meat of the poultry is more easily absorbed, and the pressure generated by the injection device in this process is smaller, and the skin is not squeezed and it is easy to pierce the bone, which alleviates the problems of high bleeding rate and high mortality rate in conventional injection conditions, and also avoids the problem of easy outflow of the injected medicine.

The utility model relates to the technical field of animal medical treatment, and discloses an angle-adjustable animal vaccine injection auxiliary device which comprises a bottom plate and an upper plate, the upper plate is arranged right above the bottom plate, a sleeve is fixed above the bottom plate, a supporting rod is arranged in the sleeve in a sliding mode, one end of the supporting rod penetrates through the sleeve and is connected to the bottom of the upper plate in a rotating mode, and the other end of the supporting rod is connected to the lower portion of the upper plate. A limiting mechanism is arranged between the supporting rod and the sleeve, an adjusting mechanism is connected between one side of the upper portion of the supporting rod and the upper plate, and a clamping mechanism is arranged above the upper plate. An operator firstly places the device at a proper position close to an animal to be injected, and the height of the device can be adjusted by adjusting the height of the supporting rod, so that the height of the device can be adjusted according to rough heights of animals with different heights and different parts.

The invention discloses a recombinant duck enteritis virus for expressing 3-type duck hepatitis A virus immunogenic genes and a construction method and application thereof. The recombinant duck enteritis virus is characterized in that an eukaryotic expression cassette containing a type 3 duck hepatitis A virus immunogenic gene is inserted into a non-coding region between UL26 and USL27 genes of a duck enteritis virus genome. According to the invention, a duck hepatitis A virus (DHAV) immunogenic gene is inserted into a duck enteritis virus (DEV) genome at a fixed point by virtue of a CRISPR / Cas9 gene editing technology, so that the recombinant duck enteritis virus for expressing the duck hepatitis A virus type 3 immunogenic gene is constructed and obtained. Moreover, based on the recombinant duck enteritis virus for expressing the 3-type duck hepatitis A virus immunogenicity gene constructed by the invention, the exogenous DHAV immunogenicity gene can be stably expressed in the recombinant virus, and the recombinant virus has good in-vitro replication ability and hereditary stability and can be used for preparing animal vaccines.