64 results about "Animals vaccines" patented technology

The invention discloses an mRNA (messenger ribonucleic acid) transcription skeleton vector and application thereof in preparation of a self-amplification type mRNA vaccine, and belongs to the technical field of veterinary biological products. According to the invention, alphavirus non-structural protein regions NSP2 and NSP3 of a self-amplification type mRNA skeleton are modified, a polyA tail is optimized, and a novel self-amplification type mRNA skeleton is constructed; the self-amplification type mRNA vaccine can improve the antigen expression efficiency, reduce the toxicity of non-structural protein to cells and improve the encapsulation efficiency and delivery efficiency by combining with the optimization of the components and proportion of a lipid nano delivery system, and meanwhile, the self-amplification type mRNA vaccine is wide in applicable pathogen antigen range and can be popularized to more animal epidemic diseases. The characteristics of high-efficiency expression, low-toxicity delivery and single-dose immunization are expected to renovate the immunization strategy of the existing animal vaccine, the epidemic prevention cost of the breeding industry is reduced, and the method has a wide industrial application prospect.

The invention provides a water-in-oil adjuvant for animal vaccines and a preparation method and application thereof, and relates to the technical field of biology, the water-in-oil adjuvant comprises 73%-75% of oil for injection, 20%-23% of an emulsifier and 2%-7% of a stabilizer; the emulsifying agent is prepared from 4.5 percent to 5 percent of polyoxyethylene fatty alcohol ether, 3.0 percent to 3.5 percent of glycerol monoisostearate, 7.0 percent to 8.0 percent of span 80, 4.5 percent to 5 percent of Tween 80 and 1 percent to 1.5 percent of vitamin E; the vaccine prepared from the water-in-oil adjuvant has the advantages that the viscosity is reduced, and the injection is easy. The technical problems that in the prior art, a vaccine prepared from an oil-in-water adjuvant is large in viscosity, so that resistance is large during injection, the occurrence rate of side reactions is high, an injection part swells, and granuloma is formed are solved.

The invention provides an animal vaccination risk prediction method and system based on a cross-modal large model, and relates to the technical field of vaccination risk prediction. Comprising a multi-modal data acquisition and integration module, a cross-modal feature extraction and semantic alignment module, a pre-training model construction and transfer learning module, a risk prediction and decision support module and a model verification and iterative optimization module. Misjudgment caused by same surface symptoms can be effectively avoided, in animal epidemic disease diagnosis, similar symptoms may be caused by different viruses, a traditional method is easily interfered by surface phenomena, and the optimized method can be used for diagnosis of animal epidemic diseases by constructing a causal diagram, analyzing real causes of symptoms and stripping confounding factors such as environment and physiological status. Through the means, the model can more accurately distinguish virus types, the misjudgment risk is remarkably reduced, and a reliable basis is provided for a subsequent vaccination strategy.

This utility model relates to the field of medical equipment technology, and in particular to an animal vaccine injection booster device, comprising a first air chamber, a second air chamber, a piston assembly, and a gun body; the gun body has a first air chamber and a second air chamber, the first air chamber having a gas source inlet, a gas outlet, and a gas delivery outlet, the gas delivery outlet communicating with the second air chamber, and a control valve also being provided in the first air chamber; when the trigger of the gun body presses the control valve, the control valve blocks the connection between the gas delivery outlet and the gas outlet; when the trigger of the gun body is released, the control valve blocks the connection between the gas delivery outlet and the gas source inlet; the second air chamber has a piston assembly, the piston assembly including a piston and a firing pin, the firing pin being disposed on the piston, and an elastic element being provided between the piston and the firing pin output end of the second air chamber; the trigger is disposed on the grip part of the gun body, the grip part having a grip groove on the side near the trigger, and a thumb groove on the top side of the grip part away from the trigger. This utility model is easy to operate and has a good grip feel.

The invention relates to a porcine bacterial polysaccharide-viral protein conjugate vaccine as well as a preparation method and application thereof, and belongs to the technical field of animal vaccines. The vaccine takes porcine bacterial polysaccharide and virus protein as raw materials, and a polysaccharide-virus protein conjugate is formed through coupling of a biotin-avidin system; wherein the virus protein is selected from one of classical swine fever virus E2 protein, porcine circovirus type 2 Cap protein, porcine pseudorabies virus gD protein and the like, and the porcine bacterial polysaccharide is selected from one of streptococcus suis capsular polysaccharide, porcine pasteurella multocida capsular polysaccharide and the like. The vaccine can simultaneously induce an organism to generate a high-level IgG antibody aiming at porcine bacterium capsular polysaccharide and virus protein, so that synergistic immune protection on porcine bacterium and virus infection is realized, and a broad-spectrum cross immune effect is achieved.

The application provides a preparation method of polysaccharide vaccine diluent and application to chicken newcastle disease vaccine, and belongs to the technical field of biopharmacy.The application provides a south five canes polysaccharide vaccine diluent and a preparation method thereof, and solves the problems of low uniformity of antibody titer, stress reaction and allergic reaction of the body after inoculation, and influence on the immunization effect of the vaccine caused by the physiological saline or the phosphate buffer solution diluent for diluting the vaccine.The south five canes polysaccharide vaccine diluent can be applied to animal vaccines, including chicken newcastle disease vaccine, and solves the problem that there is no polysaccharide diluent for the chicken newcastle disease vaccine in the prior art.

1. Name of the product in this design: Animal vaccination station (for veterinary use). 2. Purpose of this design: For vaccinating animals. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The application relates to the technical field of biological medicine, and particularly discloses CpG oligodeoxynucleotides and application thereof. The CpG oligodeoxynucleotide has a sequence as shown in any one of SEQ ID NO. 3-6, 9, and preferably has a sequence as shown in SEQ ID NO. 3-6. The CpG oligodeoxynucleotide can effectively activate the immune system of a human body or an animal, has the functions of preventing and treating pathogenic microorganism infection in a broad spectrum, has the function of improving the immunogenicity of a common vaccine, and can be applied as a human or animal vaccine adjuvant.

The invention discloses a bovine virus diarrhea live vaccine based on suspension culture and a preparation method thereof, and relates to the field of animal vaccines. The bovine viral diarrhea virus vaccine is a freeze-dried vaccine, and each vaccine contains a bovine viral diarrhea virus BVDV attenuated strain antigen and a freeze-drying protective agent; wherein the virus content of the antigen of the BVDV attenuated strain is 105-107 CCID50 / piece, and the BVDV attenuated strain is a cytopathic effect type attenuated strain suitable for suspension culture; the freeze-drying protective agent comprises trehalose, cane sugar, gelatin hydrolysate, sodium glutamate and polyvinylpyrrolidone. The suspension culture technology is adopted, large-scale, efficient and stable production of BVDV antigens is achieved, an optimized freeze-drying formula is combined, the obtained vaccine is high in virus titer, good in immunogenicity, high in stability, good in safety, advanced in production process, controllable in cost and suitable for industrial large-scale production.

The utility model discloses an animal vaccine culture medium wastewater treatment pond capable of treating waste gas, which comprises a treatment pond body, a water inlet and a water outlet, the top end of one side of the treatment pond body is provided with the water inlet, the bottom end of one side of the treatment pond body is provided with the water outlet, and the other side of the treatment pond body is connected with a waste gas treatment box in a welding way. According to the animal vaccine culture medium waste gas treatment device, adsorption force is generated in the waste gas treatment box by starting the air blower, is guided into the adsorption head under the action of the fixed pipe and the adsorption pipe, and is discharged through the adsorption head, so that waste gas generated by animal vaccine culture medium waste gas treatment is conveniently adsorbed; adsorbed waste gas is guided into the waste gas treatment box, the waste gas is filtered and purified under the action of an adsorption plate and a filter plate, and the practical environmental protection performance of the waste gas treatment device is improved through the operation, so that the influence of the waste gas on the surrounding environment and workers is reduced.

The application discloses a production method and a production system of white oil for animal vaccines, and the production method is characterized in that: firstly, raw materials are cut and separated to obtain light components and heavy components; the heavy components enter an adsorption-isomerization reaction unit to be in contact with a catalyst, and after treatment, a material flow A is obtained; when the refractive index of the material flow A is higher than that of the heavy components, the heavy components stop entering the adsorption-isomerization reaction unit; hydrogen is introduced into the adsorption-isomerization reaction unit to generate an isomerization condensation reaction, and the reaction product is further subjected to a hydrogenation refining reaction; after the reaction effluent is separated, a gas phase product, light white oil and white oil for animal vaccines are obtained. According to the method, non-ideal components are separated in advance, so that the isomerization condensation reaction is only performed on raw materials rich in ideal components, hydrogenation refining and isomerization condensation processing loads are reduced, hydrogen consumption in the process is reduced, and the economic efficiency of the device is improved.

The invention discloses a method for removing endotoxin in chicken infectious bursal disease virus VP2 protein. The method comprises the following steps: firstly, crushing a VP2 protein expression strain; adding calcium chloride into the treated VP2 protein, and standing; returning the temperature, adding sodium carbonate into the VP2 protein subjected to temperature returning, and standing; and returning the temperature of the VP2 protein added with the sodium carbonate, taking supernate, adding calcium chloride into the supernate, and standing. By setting the adding concentration of calcium chloride, the adding concentration of sodium carbonate and the treatment sequence and times of calcium chloride and sodium carbonate, the endotoxin in the VP2 protein is efficiently and rapidly removed by using calcium chloride and sodium carbonate. By adopting the method disclosed by the invention, the endotoxin removal rate reaches 99.91%, the agar diffusion titer is improved compared with that before treatment, and the animal vaccine preparation standard is met. The method disclosed by the invention is short in treatment period, large in treatment capacity, low in removal cost and suitable for industrial mass production.

The invention discloses a rapid determination method of virus content, and belongs to the field of animal vaccine quality detection. The specific process comprises the following steps: (1) preparing virus antiserum; (2) diluting a to-be-detected virus solution, and culturing for 22-24 hours; (3) based on the prepared virus antiserum, determining the FAID50 of the virus to be detected, and then determining the TCID50 of the virus to be detected; (4) judging whether the content of the virus to be detected is qualified or not according to the absolute values A of the FAID50 and the TCID50 and the p values of the FAID50 and the TCID50; if the value A is less than or equal to 0.2 and the value p is more than or equal to 0.8, judging that the content of the virus to be detected is qualified, otherwise, judging that the virus is unqualified. The constructed method is low in detection cost and short in detection period, the whole detection period is 28 hours, the problem that the virus content is reduced due to the fact that the preservation time of a semi-finished product is prolonged is solved, and the content of a finished product is increased. In addition, when the virus content is detected, a virus standard substance does not need to be used, and the experiment cost is greatly reduced.

The present invention belongs to the field of animal vaccines, and specifically relates to a multi-epitope fusion protein of Gramseria parasuis antigens targeting multiple serotypes and its application. The applicant conducted proteomic analysis on four clinical isolates of Gramseria parasuis serotypes 4, 5, and 13 with high virulence and good immunogenicity, and screened out 8 antigenic proteins. The 8 screened antigenic proteins were further analyzed, and finally an immunogenic multi-epitope fusion protein of antigens that can recognize the three serotypes was obtained. As a vaccine, the fusion protein achieved a 100% protection rate against piglets infected with the clinically isolated serotype 4 strain HB04, the serotype 5 strain SJZ05, and the serotype 13 strain GD20.

The application belongs to the technical field of animal vaccines, and particularly relates to an O-A-Dex-Ap for African swine fever immunization. Based on the good biocompatibility of Dextran, the application uses the Dextran as a nanoparticle to load a coupled CTL epitope peptide. Preliminary experimental results show that, compared with the epitope peptide alone, the O-A-Dex-Ap after loading and coupling can more effectively promote antigen presentation and activate more persistent CTL immune response, and has good application potential. Based on the results, a good technical foundation can be laid for subsequent preparation of African swine fever and other disease vaccines.

The invention discloses application of CpG ODN in preparation of a medicine for regulating animal immune cell activity, and relates to the technical field of animal vaccines. In the invention, the regulation of the animal immune cell activity is to promote the immune response reaction of immune cells; the step of promoting the immune response reaction of the immune cells comprises the step of promoting the immune cells to generate interferon. The CpG ODN provided by the invention can well and specifically stimulate immune cells of animals, especially pigs to release cytokines and promote the animals to generate rapid and strong immune response to immunogens; when being applied to an animal vaccine, the vaccine can stimulate animals to generate a higher antibody level in a shorter time, and can maintain the high-level antibody for a long time, and the immunogenicity of the vaccine is remarkably improved.

The present invention provides an aerosol protective agent for a porcine pseudorabies live vaccine, and relates to the field of animal vaccines. The aerosol protective agent is an aqueous solution containing the following ingredients: 3-24 g / L trehalose, 3-24 g / L L-arginine, 3-24 g / L proline and 1-10% glycerol, with a pH value of 7.0-7.8. The present invention also provides a method for preparing a porcine pseudorabies live vaccine aerosol, comprising: preparing a solution of a commercially available porcine pseudorabies live vaccine freeze-dried product with an aerosol protective agent, and preparing the aerosol live vaccine by ultrasonic atomization; the aerodynamic mass median particle size of the aerosol live vaccine is 1-10 μm, and after being retained in the air for 30 minutes, it still has a high titer, a high immune protection rate, simple preparation, and low cost.

The present application relates to the technical field of biological medicine, in particular to a kind of mRNA vaccine for obtaining bovine nodular skin disease virus neutralizing antibody and application thereof.The mRNA vaccine, characterized in that, contain A27L antigen selected fragment, or L1R antigen selected fragment.The mRNA vaccine provided by the present application successfully induced effective immune response in mice and cattle, detected high level of serum neutralization titer, and the effect is better than goatpox live vaccine (CVCC AV41) vaccine, and the antibody induced has no antibody-dependent enhancement effect.This breakthrough fills the blank in the current LSDV vaccine research and development field, provides a new solution for the production and research and development of animal vaccine, and has very high commercial value and broad application prospect.

The utility model relates to the technical field of animal medical treatment, and discloses an angle-adjustable animal vaccine injection auxiliary device which comprises a bottom plate and an upper plate, the upper plate is arranged right above the bottom plate, a sleeve is fixed above the bottom plate, a supporting rod is arranged in the sleeve in a sliding mode, one end of the supporting rod penetrates through the sleeve and is connected to the bottom of the upper plate in a rotating mode, and the other end of the supporting rod is connected to the lower portion of the upper plate. A limiting mechanism is arranged between the supporting rod and the sleeve, an adjusting mechanism is connected between one side of the upper portion of the supporting rod and the upper plate, and a clamping mechanism is arranged above the upper plate. An operator firstly places the device at a proper position close to an animal to be injected, and the height of the device can be adjusted by adjusting the height of the supporting rod, so that the height of the device can be adjusted according to rough heights of animals with different heights and different parts.

The invention discloses a recombinant duck enteritis virus for expressing 3-type duck hepatitis A virus immunogenic genes and a construction method and application thereof. The recombinant duck enteritis virus is characterized in that an eukaryotic expression cassette containing a type 3 duck hepatitis A virus immunogenic gene is inserted into a non-coding region between UL26 and USL27 genes of a duck enteritis virus genome. According to the invention, a duck hepatitis A virus (DHAV) immunogenic gene is inserted into a duck enteritis virus (DEV) genome at a fixed point by virtue of a CRISPR / Cas9 gene editing technology, so that the recombinant duck enteritis virus for expressing the duck hepatitis A virus type 3 immunogenic gene is constructed and obtained. Moreover, based on the recombinant duck enteritis virus for expressing the 3-type duck hepatitis A virus immunogenicity gene constructed by the invention, the exogenous DHAV immunogenicity gene can be stably expressed in the recombinant virus, and the recombinant virus has good in-vitro replication ability and hereditary stability and can be used for preparing animal vaccines.

The invention discloses a bovine virus diarrhea and rhinotracheitis bivalent inactivated vaccine and a preparation method thereof, and relates to the field of animal vaccines. The composition is prepared from the following components in parts by volume: 40-50 parts of a water phase and 50-60 parts of an oil phase, the water phase comprises a bovine viral diarrhea virus antigen solution, an infectious bovine rhinotracheitis virus antigen solution, a stabilizer, a preservative and a phosphate buffer solution; the oil phase is prepared from white oil, span-80 and tween-80. The invention adopts BPL to inactivate BVDV and formaldehyde to inactivate IBRV, the inactivation process is designed according to virus characteristics, the inactivation is thorough, the safety is high, the immunogenicity of the virus can be retained to the greatest extent, the vaccine formula is scientific, and the physical and chemical stability of the vaccine during the storage period is improved by adopting the compound stabilizer.

The utility model relates to the field of animal vaccine immunization, in particular to a portable vaccine dilution operation table which comprises a sealing bin, a transparent partition plate fixedly installed at the upper end of the sealing bin, a sealing door arranged on the front side of the sealing bin, a sealing operation glove arranged on the inner side of the sealing bin and an operation platform arranged at the lower end of the sealing bin. Self-locking universal wheels are arranged at the lower end of the operating platform, a vertical plate is arranged at the upper end of the operating platform, an illuminating lamp is fixedly installed on the front side of the vertical plate, a sprayer is arranged below the illuminating lamp, a disinfectant box is arranged on the rear side of the vertical plate, a filter box is arranged at the upper end of the rear side of the vertical plate, and a sealing cover is arranged on the rear side of the filter box. According to the utility model, the sealed operation glove is used for operation in the sealed bin, potential biological safety hazards such as vaccine spilling and aerosol diffusion are effectively avoided, and the biological safety risk is further reduced by spraying and disinfecting articles in the sealed bin through the sprayer, ventilating the sealed bin through the fan and filtering airflow through the filter element.

The animal vaccine intelligent constant-temperature preservation box comprises a preservation box body, constant-temperature refrigeration equipment is fixedly installed at the bottom of an inner cavity of the preservation box body, a refrigeration pipe is fixedly installed at the output end of the top of the constant-temperature refrigeration equipment, and a columnar visual heat preservation bin is arranged at the top of the constant-temperature refrigeration equipment in an embedded and sliding mode; a movable groove is formed in the middle of an inner cavity of the heat preservation bin, a plurality of heat preservation chambers are formed in the heat preservation bin, a through hole is formed between each heat preservation chamber and the movable groove, and refrigeration air enters the independent heat preservation chambers through the through holes, so that the vaccine tubes in the heat preservation chambers are refrigerated, and when a worker independently takes out the vaccine tubes, the vaccine tubes in the heat preservation chambers can be conveniently taken out. The sealing door can be pulled through the locking mechanism, so that a vaccine tube in the independent heat preservation chamber can be taken out and put in, the overall refrigeration effect of overall refrigeration air cannot be affected by leakage of the independent heat preservation chamber, and the constant-temperature preservation effect on vaccines is improved.

The invention belongs to the technical field of animal breeding, and particularly relates to an animal vaccination device which comprises a medicine box, a positioning frame is fixed to the bottom end of the medicine box, a medicine pumping pipe is arranged in the positioning frame, a needle is mounted at the bottom end of the medicine pumping pipe, a piston is arranged in the medicine pumping pipe, a push rod is fixed to the top end of the piston, and a movable hole is formed in the middle of the medicine box. Fixing plates are fixed to the two sides of the interior of the movable hole, lifting rods are fixed to the two sides of the top end of the pesticide pumping pipe, a lifting plate is fixed to the middles of the lifting rods, first springs are fixed to the top end of the lifting plate, the top ends of the first springs are fixedly connected with the bottom ends of the fixing plates, and a trigger assembly is arranged below the lifting plate; the needle head is positioned through the positioning frame, then the push plate is pressed to drive the clamping plate to be separated from the lifting plate, so that the first spring is released to drive the lifting rod, the lifting rod drives the needle head to be quickly inserted into an animal body, the animal positioning time is shortened, and the inoculation efficiency can be improved.

The invention relates to the field of biological medicine, in particular to the field of animal vaccines. More specifically, the invention relates to a staphylococcus aureus viable vaccine and application thereof.

The utility model discloses a parallel type injector, which relates to the technical field of medicine injection and comprises an injection mechanism, a fixing mechanism is arranged at the bottom of the injection mechanism, a pushing mechanism is arranged at the bottom of the fixing mechanism, the injection mechanism comprises three injection tubes, the front ends of the three injection tubes are fixedly connected with injection needles, and the injection needles are connected with the fixing mechanism. The device comprises three injection pipes, a parallel rod is fixedly connected between every two adjacent injection pipes, two limiting vertical plates are fixedly installed on the rear sides of the tops of the three injection pipes, rubber pushing heads are connected to inner rings of the three injection pipes in a clamped mode, pushing rods are fixedly installed at the rear ends of the three rubber pushing heads, and pushing blocks are fixedly installed at the rear ends of the three pushing rods. According to the utility model, the injection mechanism and the pushing mechanism are matched with each other, so that the three injectors which are fixed in parallel can synchronously realize extraction and injection work, the frequency of repeated injection is reduced, the efficiency of animal vaccine injection is improved, and the labor intensity is reduced.

The application belongs to the technical field of animal vaccine preparation, and discloses an African swine fever virus (ASFV) multi-epitope fusion protein, a ferritin nanoparticle vaccine of the ASFV multi-epitope fusion protein and application. The application obtains 16 dominant B cell epitopes by using an ASFV phage display library in combination with reverse screening of clinical positive serum, and constructs a nanoparticle vaccine by linking the 16 epitopes with ferritin self-assembly. The vaccine has excellent biosafety, can induce high-level and sustained specific antibodies and significant IFN-gamma cellular immune response in pigs, can effectively delay the clinical onset and death process of pigs in ASFV virulent challenge experiments, and can significantly reduce the virus load in whole-body organ tissues, thereby providing 25% survival protection rate. The application provides an effective new strategy for the development of a subunit vaccine for responding to various ASFV mutant strains.

The invention belongs to the technical field of animal vaccine diluting devices, and particularly relates to a diluting device and method for preparing animal vaccine.The device comprises a stirring barrel, the top of the stirring barrel is communicated with a first feeding part and a second feeding part, a ventilation stirring part is arranged in the middle of the inner side of the stirring barrel, and the air inlet end of the ventilation stirring part is communicated with an air supply assembly; the air outlet end of the ventilation stirring part is communicated with the interior of the stirring barrel, the stirring barrel is communicated with the air inlet end of a filter, and the air outlet end of the filter is communicated with one air inlet end of an air supply assembly through a reversing valve; the ventilation stirring part comprises a hollow rotating pipe rotationally arranged at the axis of the inner side of the stirring barrel, the bottom end of the hollow rotating pipe communicates with the air outlet end of the air supply assembly, a plurality of stirring pipes are arranged on the hollow rotating pipe in a matrix surrounding mode, the stirring pipes communicate with the hollow rotating pipe, and a plurality of air holes formed in rows are formed in the stirring pipes; the axis of the air hole is horizontally arranged. The invention further discloses a using method of the device.

The invention provides a polymer composite adjuvant for an animal vaccine and a preparation method thereof, and the polymer composite adjuvant for the animal vaccine comprises acrylate hydrogel, nano oil emulsion and an immunopotentiator, the acrylate hydrogel is prepared by the following steps: generating a polyacrylate precipitate from an acrylic hydrophilic monomer and an acrylic hydrophobic monomer in a solvent by adopting a precipitation polymerization method, and immersing the precipitate in ultrapure water, the hydrophilic monomer is one of acrylic acid and methacrylic acid, and the hydrophobic monomer is acrylate with the carbon chain length of 10-22; the immunopotentiator is a TLR3 (toll-like receptor 3) or TLR9 (toll-like The polymer composite adjuvant disclosed by the invention has good biocompatibility, low mineral oil content and small side effects, not only can play an antigen slow release role, but also can improve the cellular immune response of an organism, has the average particle size of 40-60 nm, and is good in dispersity, and a prepared vaccine is low in viscosity and easy to inject; and the preparation process is simple, the stability is high, and the biological safety is good.