10 results about "Oil adjuvant" patented technology

The invention discloses a recombinant tetanus toxin protein rTT as well as a preparation method and application thereof, the recombinant protein sequentially contains a His tag, a modified exogenous polypeptide and two tetanus toxin fragments connected in series through flexible polypeptides from the N end to the C end, the two tetanus toxin fragments connected in series form two intramolecular disulfide bonds in a molecule through double mutation, and the tetanus toxin fragments are connected in series through flexible polypeptides. Coding nucleotide of the recombinant protein is expressed by escherichia coli BL21 (DE3), induced at low temperature and purified, and target protein with the purity larger than or equal to 95% can be obtained. A subunit vaccine prepared from the protein and a two-way oil adjuvant ISA201 enables mice to achieve 100% protection under 100 LD50 challenge dose, the protection effect is better than that of truncated tetanus toxin heavy chain fragment TTc protein, and the recombinant protein can be used for tetanus prevention vaccines and serological diagnostic reagents.

The invention belongs to the technical field of biology, mainly relates to a preparation method of a composite adjuvant, and particularly relates to a water-soluble immune composite adjuvant, a vaccine and a preparation method of the vaccine. According to the compound adjuvant, every 300 mL of the compound adjuvant is prepared from 30 mL to 50 mL of carbomer stock solution, 30 mL to 37.5 mL of polyinosinic stock solution, 800 mg to 900 mg of triterpenoid saponin, 1.2 g of chitosan oligosaccharide and 20 mL of chitosan solution. Wherein the concentration of the carbomer stock solution is 30 mg / mL, the concentration of the polyinosinic-polycytidylic acid stock solution is 50 mg / mL, and the concentration of the chitosan solution is 600 mg / mL. The water-soluble immune composite adjuvant disclosed by the invention does not contain mineral oil components, granuloma of an injection part caused by viscous oil adjuvant vaccines is avoided, the water-soluble immune composite adjuvant is conveniently mixed with antigens in use, the economic benefits and the use convenience of farmers are effectively improved, and the water-soluble immune composite adjuvant is easy to industrially produce. And meanwhile, the gleditsia sinensis saponin enzymatic hydrolysate and the chitosan oligosaccharide enzymatic hydrolysate contained in the adjuvant have a synergistic effect, so that a relatively strong immune effect is achieved, humoral immune response and cellular immune response can be stimulated, and the immune persistence can be enhanced.

The invention provides an application of an aeromonas hydrophila Ah-221212 strain in preparation of a vaccine for preventing septicemia of aquatic animals caused by aeromonas hydrophila. The inactivated vaccine prepared from the specific aeromonas hydrophila Ah-221212 strain has the advantages of simple preparation steps and low cost, can have multiple functions of targeted delivery, self-adjuvant effect, slow release and the like, does not need to additionally add traditional adjuvants such as an aluminum adjuvant and an oil adjuvant, and is a novel aeromonas hydrophila aeromonas hydrophila vaccine with excellent performance.

The present application relates to the field of biological medicine, and particularly relates to a recombinant herpes zoster vaccine composition and a preparation method thereof.The recombinant herpes zoster vaccine composition comprises gE protein and an adjuvant, the adjuvant is squalene oil-in-water, and the squalene oil-in-water adjuvant comprises the following concentration components: squalene: 4-5 wt%; Tween 80: 0.2-0.8 wt%; Span 85: 0.2-0.8 wt%. The vaccine composition can specifically enhance the cellular immune response to varicella-zoster virus, can significantly improve the expression of cytokines such as INF-gamma and IL-2 of CD4+ T cells in mice, and can improve the level of humoral and cellular immune response, shows high immunocompetence for preventing varicella-zoster virus infection in clinic, and the components in the vaccine composition are cheap and easy to obtain, effectively improve the yield of the vaccine, and reduce the cost of the vaccine.

The invention discloses an oil-in-water adjuvant for vaccines and a preparation method of the oil-in-water adjuvant. The oil-in-water adjuvant for the vaccine is prepared from isopropyl myristate, alpha-tocopherol, trehalose, an oil-phase mother solution and a PBS (Phosphate Buffer Solution). The invention also provides a preparation method of the composition. Compared with the prior art, the oil-in-water adjuvant for the vaccine, which has long-term physical stability, controllable dropping diameter, low foaming and controlled oxidation under the condition of 2-8 DEG C, is constructed on the premise of reducing the intervention of a small molecular surfactant as much as possible, and meanwhile, the compatibility and operability with an antigen system are ensured.

The invention provides an adjuvant for an oil-in-water animal vaccine. The adjuvant contains 8.8%-19.6% of mineral oil, 3.7%-6.6% of span 80, 6.1%-11.0% of Tween 80, 3.9%-7.0% of glycerol, 2.9%-5.3% of polyglycerol ester and the balance of water for injection, wherein the total percentage of the adjuvant is 100%. The oil-in-water adjuvant disclosed by the invention is simple in formula and simple in preparation method; the animal vaccine prepared by utilizing the adjuvant is good in physical stability, can be stored for more than 12 months at 4 DEG C, can be stored for 12 months at 25 DEG C and can be stored for 2 months at 37 DEG C, and the storage stability is obviously superior to that of similar products at home and abroad; the animal vaccine prepared from the adjuvant is low in viscosity and easy to inject, no visible residue exists in animal experiments, death or obvious adverse reaction caused by vaccine injection is avoided, and guarantee is provided for vaccine injection safety and effectiveness and economic animal food safety.

The application relates to the technical field of vaccine adjuvants, and discloses an oil-in-water adjuvant, a preparation method and application thereof. The adjuvant comprises an oil phase, a composite surfactant, an interface polarity adjusting component and an aqueous phase; the interface polarity adjusting component is 1,3-butanediol; the composite surfactant is composed of polyoxyethylene hydrogenated castor oil with a polyoxyethylene unit number of 35 to 45 and sorbitan monooleate; the hydrogen bond anchoring network formed by 1,3-butanediol and the polyoxyethylene segment is utilized to increase the interface desorption energy barrier and improve the interface film expansion modulus, to inhibit the coalescence of the oil phase droplets under the condition of ultrahigh dilution and low shear, to spontaneously maintain the nanoscale particle size distribution, the static interface structure constructed by the application effectively offsets the physical failure caused by dilution, reduces the system kinetic viscosity to reduce injection stress, and cooperatively improves the antigen loading specific surface area and the immune induction effect.

The utility model discloses an oil adjuvant inactivated vaccine mixing equipment relates to inactivated vaccine mixing technical field, including heating jar, the inside cooperation and installation of heating jar have mixing jar, the bottom of mixing jar is conical structure, the bottom of mixing jar is equipped with the liquid outlet, the bottom fixed mounting of heating jar has the transfusion pipe, the bottom setting of heating jar installs and is equipped with filling assembly, the top setting of heating jar installs and is equipped with stirring assembly. The utility model design scheme is through the bottom of mixing jar conical structure's design, the bottom of mixing jar is equipped with the liquid outlet, under the action of filling assembly, the vaccine in mixing jar is discharged outward through the liquid outlet and fills, the vaccine in mixing jar will be under the direction of the conical surface, all from the discharge of mixing jar, avoid the vaccine residual in mixing jar, thereby avoid the waste condition to take place.

The application provides a water-in-oil adjuvant for poultry animal vaccines and a preparation method and application thereof, and belongs to the technical field of biological product animal vaccines. The raw materials of the water-in-oil adjuvant provided by the application include, in percentage by mass, 85wt%-90wt% of injection oil, 5wt%-10wt% of refined Span-80 and 1wt%-5wt% of refined Tween-80, and can further include 0.1wt%-1wt% of an immune stimulating complex. The vaccine prepared by using the water-in-oil adjuvant provided by the application is stable in quality, high in safety, and can induce the body to produce an immune response with a longer duration and higher efficiency, and therefore can be used as a safe and effective adjuvant for poultry animal vaccines and the like.

The invention relates to the technical field of vaccine immunization, in particular to a compound oil adjuvant as well as a preparation method and application thereof. The compound oil adjuvant is prepared from the following components in percentage by mass: 1 to 6 percent of squalene, 0.5 to 3.0 percent of a surfactant, 0.02 to 0.5 percent of a nano slow-release agonist, 0.1 to 1.2 percent of a biodegradable polymer, 3 to 7 percent of an isotonic agent and the balance of a buffer solution, wherein the nano sustained-release agonist jointly encapsulates a Mincle agonist trehalose 6, 6 '-behenate and a TLR4 agonist CRX-675 through a lipid carrier, a fucoidin and sodium hyaluronate stabilizing system is adopted, and the nano sustained-release agonist is integrated into the squalene emulsion after being subjected to surface modification by a biodegradable polymer. Targeted delivery and slow release of double agonists are realized, and an immune signal channel is synergistically activated, so that the immune response of vaccine body fluid and cells is remarkably improved, and excellent physical stability and safety are achieved. The invention further discloses a preparation method of the adjuvant and application of the adjuvant in preparation of inactivated vaccines.