33results about How to "Guaranteed immune effect" patented technology

The invention discloses a follow-up infant formula which relates to a formula. The follow-up infant formula improves the immunity, promotes development of nerves and retina, enhances the resistance of body against pathogen, and promotes lipid metabolism and nutrition balance. Each ton of the follow-up infant formula disclosed by the invention consists of 638 kg of dry substances in raw milk serving as a raw material, 237 kg of desalted whey powder, 60 kg of soybean oil, 53 kg of fructooligosaccharide and 12 kg of composite nutrient; and the raw materials are mixed and subjected to sterilization concentrating and spray drying processes to obtain the follow-up infant formula. The follow-up infant formula disclosed by the invention has an active effect on the immune system, improves the immunity, promotes development of nerves and retina, enhances the resistance of body against pathogen, and promotes lipid metabolism and nutrition balance and the like. The follow-up infant formula disclosed by the invention is suitable for the infants of 6-12 months old.

The invention discloses a porcine epidemic diarrhea virus genetic engineering vaccine and a preparation method thereof, which belongs to the field of veterinary biological products. Core proteins of hepatitis B virus are used as a vector, a porcine epidemic diarrhea virus protective antigen is inserted between a 79 amino acid to 80 amino acid of the vector by using a molecular biology method to form the genetic engineering vaccine for preventing the porcine epidemic diarrhea virus. The preparation method of the vaccine is simple, a great amount of antigen proteins can be prepared, theconsumed time is short, the expression amount is high, the production cost is greatly reduced, and the mass production is facilitated. The porcine epidemic diarrhea sub-unit vaccine comprising HBcAg-PEDV-COE recombinant proteins prepared in the invention is good in immunity effect, small in immunity dose, and capable of effectively preventing the infection of the porcine epidemic diarrhea virus.

The invention provides an H9 subtype avian influenza virus inactivated vaccine including chicken a-interferon protein. The H9 subtype avian influenza virus inactivated vaccine comprises an antigen and a vaccine adjuvant, and the adopted antigen is an inactivated H9 subtype avian influenza virus QDY1 strain including the chicken a-interferon protein. A screened H9 subtype avian influenza virus has the advantages of being small in virulence and high in immunogenicity. The H9 subtype avian influenza virus (QDY strain) is inoculated into a chick embryo, then collects virus liquid, and is mixed with the extracted and purified chicken a-interferon protein after inactivation of formaldehyde solutions, and an oil adjuvant is added for emulsification to manufacture the vaccine. The prepared vaccine can stimulate an organism to generate an antibody fast, the level of the antibody is improved, the persistent period of the antibody is prolonged, and diseases caused by the H9 subtype avian influenza virus is prevented. The vaccine has the advantages of being efficient and good in safety.

The invention discloses non-reactive high-efficiency feed for piglet early weaning. The feed consists of corns, broken rice, wheat middling, soybean meal, extruded soybeans, fermented soybean meal, fish meal, domey protein powder, low protein whey powder, chocolate feed powder, cane sugar, soybean oil, mountain flour, calcium hydrogen phosphate, salt, choline, nome selenium, zinc oxide, feed-bond, lysine, methionine, threonine, tryptophan, healthy acid, weaning powder, a sweetening agent, peony fragrance, feed complex enzyme, an antioxidant Yangliting, pig mineral substance premix and pig vitamin premix. According to the feed disclosed by the invention, the weaning stress, nutritional stress, temperature stress and environmental stress of the piglets can be effectively reduced, and the economic benefits are improved.

The invention relates to a w / o / w adjuvant composition. The w / o / w adjuvant composition comprises a sucrose fatty acid ester with HLB value of below 5, fatty alcohol-polyoxyethylene ether and oil. The vaccine composition prepared by the adjuvant composition is stable at different temperature and has no layering and demulsification phenomena, and the preparation condition of the vaccine is simple andmild. The prepared vaccine achieves the immune effect rapidly and does not have adverse effect during use.

The invention provides a preparation method of an inactivated lyophilized vaccine for porcine circovirus type 2. The preparation method comprises the following steps: successively carrying out clarification procedure, ultrafiltration concentration and molecular sieve chromatography column purification on a virus culture solution, so that the impurity content of an antigen used for preparing the vaccine is effectively ensured to be reduced to the minimum, thus avoiding the phenomenon that side effects are generated after the product is used, and also being beneficial to ensuring the immune effect of the vaccine. In addition, according to the preparation method provided by the invention, as the inactivated antigen is directly prepared in a lyophilizing mode without adding an oil adjuvant, the problems of difficulty in injection and absorption do not exist. The PCV 2 inactivated lyophilized vaccine prepared by the preparation method is high in antigen content, immunogenicity and antigen purity, is good in safety, is low in side effect, is capable of rapidly generating antibody protection without an adjuvant and is easily injected and absorbed.

The invention relates to a process for preparing antibody for small molecular substance using nanoliposome immune adjuvant, which belongs to the field of immunologic technique, including mixing soybean lecithin and cholesterol according to a weight ratio of 1.8-2.5 / 1, dissolving the mixture into a methylene dichloride solvent with W / V at 1 / 500-800, adding into a flask, vacuum evaporating at a temperature from 35 to 40 DEG C. on a rotary evaporator; charging an antigen solution with concentration ranging from 1.5 to 2.0mg / mL, and ratio to the embedding material ranging from 2.0 to 2.5 / 1, strongly swinging, wherein 20mM PBS with pH ranging from 6.8 to 7.2 is used as buffer; intravenous immune is carried out at the amount of 200-300 mu g per mice on each time; secondary booster immune; selecting mouse with high special antibody potency for cell fusion experiment, screening needed monoclonal antibody. The invention has advantage that: (1) the antibody is great in solubility with the embedding material, easy to be dissolved in the animal body and non-toxic; (2) liposome is rapidly captured by an immune cells when introduced into peripheral blood and distributed to lymph nodes and central immune organs, and antigen presenting is realized; (3) the antigen is embedded into the liposome, so that stability of antigen is increased, and immune effect is ensured.

The invention provides application of swine fever and porcine pseudorabies live vaccines to preparation of medicaments for treating or preventing swine fever and porcine pseudorabies. The swine fever vaccine and porcine pseudorabies vaccine are used for immunization of piglets through nasal inhalation. The swine fever and porcine pseudorabies live vaccines can effectively prevent swine fever virus and porcine pseudorabies of piglets, at the same time significantly reduce the occurrence of porcine respiratory disease, and effectively prevent respiratory syndromes.

The invention provides a single-frame fringe projection three-dimensional surface type measurement method, wherein the method comprises the following steps: S1, constructing a deep convolutional neural network which has six paths, and defining the deep convolutional neural network as DL-net. According to the method, firstly, a deep learning method is utilized, high-quality phase information extraction of a single-frame fringe image at three camera visual angles is realized under the driving of a large amount of data, then, a calibration space epipolar relationship among multiple visual angles is utilized, and the similarity wrapping the phase information is used as measurement to realize robust phase unwrapping, and then the high precision absolute depth information of a measured object can be recovered; and the powerful feature extraction advantage of deep learning and the efficient phase unwrapping advantage of geometric constraint are combined, and high-quality phase extraction, robust phase unwrapping and high-precision absolute depth recovery are achieved on the premise of single-frame projection.

The invention belongs to the technical field of livestock breeding, and particularly relates to a Northern Guizhou Ma goat breeding epidemic disease prevention and control technology. The technology comprises the following steps that S1, a site is selected, and leymus chinensis is planted; S2, epidemic disease preventing medicine is prepared; S3, epidemic disease preventing forage grass is processed and prepared, medicine A and harvested and chopped forage grass are mixed to be uniform according to the proportion of 1:6, then ensiling, ammoniation and fermentation are conducted in sequence toobtain the epidemic disease preventing forage grass, medicine B is uniformly irrigated on outdoor farm grass through a water spraying device at night every 2-3 days, on the second day after irrigationis completed, and forage grass in the area not eaten in a short time is harvested and serves as epidemic disease preventing forage grass raw materials; S4, disinfection and source destroying are conducted; S5, desinsectization and medicated bath are conducted; S6, immunization is conducted. The technology is scientific, reasonable, high in prevention and control density and good in prevention andcontrol effect, fattening goats for slaughter do not have any infectious diseases or other diseases, the prevention and control density reaches 100%, and no dead angle exists.

The invention discloses a formula of a traditional Chinese medicine for resisting diseases, enhancing immunity and improving production performance of meat poultry. The traditional Chinese medicine isprepared from raw materials in parts by weight as follows: flos chrysanthemi, semen cassiae, fructus lycii, pearl, herba menthae, common macrocarpium fruit, poria cocos, cortex moutan radicis and rhizoma atractylodis. The traditional Chinese medicine has the following beneficial effects: the traditional Chinese medicine prevents and resists pestilences and has remarkable prevention and treatmenteffects on infectious proventriculitis, ventriculitis, colibacillosis, salmonellosis, enterotoxin syndrome, air sacculitis and other diseases; drug resistance is reduced, drug sensitivity is recovered, and bacteriosis can be controlled easily; immunosuppression is removed, the vaccination effect is guaranteed, and stress response to a vaccine is reduced; after the traditional Chinese medicine is used in the whole feeding period, the poultry meat contains little water, is rich in vitamins and amino acids and low in cholesterol content and has good flavor.

The invention discloses an immunization method of chicken embryos, comprising the following immunization steps of (1) sealing, steaming and disinfecting the immunization space with potassium permanganate and formalin in each cubic meter; (2) disinfecting the chicken embryo air-chamber with alcohol of which the concentration is 75%; (3) candling the chicken embryos of which the age is 18-19 days to screen the dead eggs or the unfertilized eggs; (4) diluting the immunization vaccine with 200-220 milliliters of diluent in each 4000 dose; (5) immunizing the single strain with the immunization chicken embryo with the diluted vaccine, wherein the immunization part is the air-chamber part of the chicken embryo; and (6) sealing, which is to seal the immunization needle holes of the chicken embryo eggs which are immunized. In the invention, the immunization space and the immunization parts are firstly disinfected, so the infection possibility is reduced, and the temperature and the humidity of the immunization space are stabilized; and because of the immunization sealing, the immunization effect is ensured, the stress death of the chicken embryos is prevented, and the hatch loss is reduced.

The invention provides a novel coxsackie virus group A 6 recombinant subunit protein vaccine and a preparation method thereof. The invention relates to the novel coxsackie virus group A 6 recombinantsubunit protein vaccine which is artificially designed. The vaccine comprises a recombinant protein formed by fusion design of a diphtheria toxin non-toxic mutant CRM197 protein with an immunologic adjuvant effect and a CV-A6 antigen structural protein. The recombinant protein is subjected to escherichia coli expression, chromatographic column separation, purification and compatibility, and finally the recombinant subunit protein vaccine with good immunogenicity is prepared. The vaccine can effectively activate an organism to generate a neutralizing antibody aiming at the coxsackie virus groupA 6, and can effectively prevent the infection of the coxsackie virus group A 6 on an organism. The vaccine disclosed by the invention realizes efficient expression of the target protein in an escherichia coli expression system by carrying out password liberation on an expression gene, so that the preparation efficiency is greatly improved, the production cost is reduced, and a foundation is laidfor popularization and immunization of large-scale crowds.

The invention provides a vaccine composition containing a swine mycoplasmal pneumonia antigen and a swine streptococcosis antigen, and a preparation method and an application thereof. The vaccine composition includes an immunizing dose of the swine mycoplasmal pneumonia antigen, an immunizing dose of the swine streptococcosis antigen, and an adjuvant. The vaccine composition has a simple immunization program, can effectively control the swine mycoplasmal pneumonia antigen and the swine streptococcosis antigen, has an immune effect equivalent to the immune effect realized through respective injection of single vaccines, and also has the characteristics of small side reaction, long immune period, less time consumption, and less labor consumption; and the vaccine composition also has the advantages of simple production technology, low immune cost and strong practicality.

The invention discloses an anti-Hib-RSV-meningococcus combination vaccine comprising a Hib-meningococcus intermediate and an RSV immune intermediate, wherein the meningococcus immune intermediate comprises modified delta fHbp protein, a flexible linker peptide segment and NadA protein, the recombinant delta fHbp protein comprises VA and VB domains of fHbp variant V1 and VC, VD and VE domains of fHbp variant V3, and Hib capsular polysaccharides are conjugated to the delta fHbp protein and / or the NadA protein after being activated; the antigen of the RSV immune intermediate is an RSV membrane surface fusion protein F and / or attachment protein G which can cause body immune response, the nucleic acid sequence expressing the protein antigen is recombined on a nucleic acid vector, the nucleic acid sequence of the recombinant protein takes attenuated intracellular bacteria as a bacteria vector; the immune intermediates of the components are separately prepared and then mixed for use.

The invention provides a canine distemper virus strain, a bivalent vaccine based on a canine distemper virus and a canine parvovirus and application of the bivalent vaccine, and belongs to the technical field of vaccines. The biosafety problem of bivalent vaccine virus seed enhancement and the immune protection effect problem of young dogs are solved. The invention provides a canine distemper virus which is named as a canine distemper virus CDV-SN strain and has a preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.23205. The invention also provides a bivalent vaccine based on the canine distemper virus and the canine parvovirus disease, and the bivalent vaccine comprises a stock solution of the canine distemper virus CDV-SN and a stock solution of recombinant baculovirus CPV-2b-VP2, and the mixing ratio of the stock solution to the stock solution of the recombinant baculovirus CPV-2b-VP2 is 1: 1. Compared with the existing product canine distemper virus and parvovirus disease bivalent live vaccine, the product has the advantages of good safety, high antigen concentration and high purity, and can be used as a preferred vaccine for basic immunity of puppies to reduce the influence of maternal antibodies on the immune effect.

The invention discloses a recombinant herpesvirus turkey live vector vaccine capable of simultaneously expressing classical strain and variant strain infectious bursal disease virus VP2 protein, and belongs to the field of veterinary biological products. According to the invention, a VP2 protein and LTB fusion expression cassette containing an infectious bursal disease virus classical strain is knocked into a US2 virus replication non-essential region of a turkey herpesvirus, and a VP2 protein and LTB fusion expression cassette of a variant strain is inserted into a US10 virus replication non-essential region. The finally obtained recombinant virus simultaneously and efficiently expresses LTB-VP2 fusion antigen protein of the IBDV classic strain and the variant strain in CEF cells. The vaccine prepared by the invention can improve the antibody level after immunization, improve the uniformity of the antibody after immunization and ensure the immunization effect of the vaccine, has the advantages of high efficiency, good safety and lifelong immunization after one-time inoculation, does not cause clinical reaction and pathological damage to chicks, and has the vaccine protection rate of 100%.

The invention belongs to the field of biological medicine and discloses an artificial avian paramyxovirus. The genome of the artificial virus contains a backbone sequence (which is the genomic sequence of a certain avian paramyxovirus) and a partial sequence of an influenza virus hemagglutinin gene (which does not encode a polypeptide anchored to the viral envelope). Unlike previously similar artificial avian paramyxoviruses, the artificial avian paramyxovirus does not alter their capsular structure. Therefore, if the artificial virus is used as an influenza vaccine (being a live vaccine) strain for humans and animals, it can be ensured that the artificial virus normally proliferates in an inoculated animal in terms of the structure of the viral envelope, thereby ensuring its immune effect. In addition to inserting partial sequences of influenza virus genes into the genome of the artificial virus, it is also possible to insert a protein coding sequence of another class of viruses, so that an animal inoculated with the artificial virus also produces a specific immune response against another type of viruses.

The invention aims at providing an H9 subtype avian influenza virus strain. Vaccine is prepared by the virus strain to solve the problem that existing H9 subtype avian influenza virus vaccine is poor in immune effect caused by a novel virus. The H9 subtype avian influenza virus QDY strain (Avian Influenza Virus) is preserved in the typical culture preservation center in China in Wuhan University on April 29, 2015, and the preservation number is CCTCC NO: V201517. After the H9 subtype avian influenza virus (QDY strain) is inoculated to a chick embryo, a virus solution is collected, after ultrafiltration concentration and formaldehyde solution inactivation are performed, oil adjuvant is added, and then mixing and emulsifying are performed to prepare the vaccine. According to the prepared vaccine, the antibody level after immunization can be improved, the antibody uniformity after immunization can be improved, and the immune effect of the vaccine can be guaranteed; the vaccine has the advantages of being efficient and good in safety.

The invention discloses an apparatus for receiving and replacing syringe needles for animals, which comprises a main shaft, a nut, a pin, containing grooves, a frame for receiving and replacing needles, marks, a handle, a base, syringe needles, an upper cover, a nick of the upper cover, a spring, a concave and convex contact face at the upper end of the frame for receiving and replacing needles, and a annular groove. Pairs of containing grooves that are considered as main units for containing as well as receiving and replacing needles are arranged on the frame for receiving and replacing needles, and each pair of the containing grooves can contain sterilized needles and used needles respectively. The application of the apparatus for receiving and replacing syringe needles for animals can meet the requirements of 'one animal, one syringe needle' on animal epidemic prevention of China, which avoids the spread of epidemic caused by replacing needles through hand clamps in the traditionalinjection method. The apparatus for receiving and replacing syringe needles for animals not only can render a quick replacement of vaccination and medical needles, improve the working efficiency of hand-operated needle replacement; but also can avert any possibility of spread of animal epidemic caused by the repeated use of needles in the process of disease prevention and treatment; and at the same time meet the requirements of using the vaccination and liquid medicine in a limited time to reduce the losses caused by the failure of immunization and decline of drug effect.

The invention discloses a dynamic analyse system of network alarm which is used for dynamic analysing influence degree of alarm to service network. The dynamic analyse system includes: the corresponding service analyzer is used for generating corresponding service link list; the alarm frequency analyzer is used for receiving alarm information came from the alarm influence degree analyzer and timesection used for calculating alarm frequency, and calculates alarm frequency generated in present alarm port; the alarm influence degree analyser is used for colligating present alarm information andthe alarm frequency to calculate influence degree of present alarm. Comparing with prior art, the system and method provided by the invention can make manager finding key problem of threatening and influencing network developing smoothly. On the one hand, manager can achieve maximum results with little effort and reduce labour cost, on the other hand, recovery capability and immunity capability of network can be solved early.

The invention aims at providing porcine parvovirus subunit vaccine. By means of the porcine parvovirus subunit vaccine, the problem that existing vaccine is not good in immune effect is solved. Antigen used in the vaccine is porcine parvovirus VP2 protein expressed by recombined pichia pastoris X33-VP2, the strain was preserved in China Center for Type Culture Collection on 9, Marh, 2016, and the preservation number is CCTCC M 2016098. According to the porcine parvovirus subunit vaccine, after a culture medium is inoculated, the recombined pichia pastoris X33-VP2 is subjected to inducible expression, after extraction, purification and inactivation via a formaldehyde solution are conducted, oil adjuvant is added, and emulsifier is mixed, so that the vaccine is obtained. By means of the subunit vaccine, uniformity of immunized antibody can be improved, the immune effect of the vaccine is guaranteed, and the vaccine has the advantages of being high in efficiency and good in safety.

The invention provides a bivalent vaccine of an acian metapneumovirus and an H9subtype avian influenza virus. The bivalent vaccine comprises an antigen and an vaccine adjuvant, wherein the antigen is an H9 subtype avian influenza virus strain and acian metapneumovirus F protein; thepreservation numberof the H9 subtype avian influenza virus strain is CCTCC NO.V201517; and the amino acid sequence of the acian metapneumovirus F protein is SEQ ID NO:1. The H9 subtype avian influenza virus strain QDY and the acian metapneumovirus F protein antigen solution are mixed at the ratio, and then an oil adjuvant is added to the mixture to be mixed and emulsified into the vaccine. According to the prepared vaccine, the immunized antibody level can be improved, the uniformity of the immunized antibody is improved, the immune effect of the vaccine is ensured, and the vaccine has the advantages of high efficiency and good safety.

The invention provides a vaccine composition containing a swine mycoplasmal pneumonia antigen and a swine streptococcosis antigen, and a preparation method and an application thereof. The vaccine composition includes an immunizing dose of the swine mycoplasmal pneumonia antigen, an immunizing dose of the swine streptococcosis antigen, and an adjuvant. The vaccine composition has a simple immunization program, can effectively control the swine mycoplasmal pneumonia antigen and the swine streptococcosis antigen, has an immune effect equivalent to the immune effect realized through respective injection of single vaccines, and also has the characteristics of small side reaction, long immune period, less time consumption, and less labor consumption; and the vaccine composition also has the advantages of simple production technology, low immune cost and strong practicality.

The invention discloses a subunit vaccine for preventing type-IV aviadenovirus as well as a preparation method and application thereof, which belongs to the field of veterinary biological products. The preparation method comprises the following steps: cloning and amplifying a C-end and an N-end structural domain genes of FadV 4C Hexon genes; establishing a recombinant baculovirus rBac-HEXON-P10-C-PH-N capable of co-expressing two antigen proteins by using an insect cell-baculovirus bidirectional expression system, wherein the recombinant virus efficiently expresses the FAdV 4C Hexon-C and Hexon-N antigen proteins in the insect cell HF; and extracting, purifying, BEI inactivation, and emulsifying by adding adjuvant, thus obtaining the vaccine. The preparation method is simple, a great amount of FAdV 4C Hexon-C and Hexon-N proteins can be simultaneously prepared, the consumed time is short, the expression amount is high, the production cost is greatly reduced, and the mass production canbe realized. The obtained recombinant subunit vaccine is good in immunity effect, small in immunity dose and capable of preventing the infection of the aviadenovirus.

The invention relates to a w / o / w adjuvant composition, which comprises a sucrose fatty acid ester composition with an HLB value of 5 or less, fatty alcohol polyoxyethylene ether and oil. The vaccine composition used for the preparation of the adjuvant composition of the present invention is stable at different temperatures without delamination and demulsification, and the preparation conditions of the vaccine are simple and mild. The prepared vaccine quickly achieves the immune effect, and the vaccine has no adverse reaction when used.

The invention provides a type B acian metapneumovirus subunit vaccine.The type B acian metapneumovirus subunit vaccine is prepared from an antigen and a vaccine adjuvant, wherein inactivated type B acian metapneumovirus F protein serves as the antigen, and the amino acid sequence of the type B acian metapneumovirus F protein is SEQ ID NO:1.According to the type B acian metapneumovirus subunit vaccine, genetic engineering pichia pastoris X33-F for expressing the type B acian metapneumovirus F protein is inoculated into a culture medium, the type B acian metapneumovirus F protein is efficiently expressed under the action of methyl alcohol, the type B acian metapneumovirus F protein is extracted and purified and then added into a formaldehyde solution to be inactivated, then an oil adjuvant is added for mixing emulsification, and the vaccine is prepared.The prepared vaccine can increase the level of an immunized antibody and improve the uniformity of the immunized antibody, and the immune effect of the vaccine can be ensured; the vaccine has the advantages of being efficient and good in safety.