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Artificial avian paramyxovirus

A myxovirus and virus technology, applied in the field of influenza vaccine strains, can solve the problems of reducing the immune effect of vaccines and achieve the effect of ensuring the immune effect

Inactive Publication Date: 2019-06-25
CHINA ANIMAL HEALTH & EPIDEMIOLOGY CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, if such man-made viruses are used as vaccine (referring to live vaccine) strains, the proliferation of the virus may be inhibited in the vaccinated animals, which may reduce the immune effect of the vaccine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The artificial avian paramyxovirus described in Example 1 uses the genome of type 2 avian paramyxovirus as the genome backbone. Its production takes the following steps: first step, prepare T7 cell line (capable of expressing T7 RNA polymerase), prepare plasmid N (capable of expressing type 2 avian paramyxovirus N gene in eukaryotic cells), prepare plasmid P (capable of expressing in eukaryotic cells) Expression of type 2 avian paramyxovirus P gene in eukaryotic cells), preparation of plasmid L (capable of expressing type 2 avian paramyxovirus L gene in eukaryotic cells), preparation of plasmid G (plasmid G contains T7 RNA polymerase promoter, It also contains the complete genome of avian paramyxovirus type 2. The genome inserts the signal peptide sequence and HA1 sequence of the HA gene of H5 subtype avian influenza virus and the stop codon; the insertion site is in the P gene and Between the M genes; after the partial sequence of the HA gene of the H5 subtype avian in...

Embodiment 2

[0019] The artificial avian paramyxovirus described in Example 2 also uses the genome of type 2 avian paramyxovirus as the backbone of the genome. It differs from Example 1 only in that: the HA2 sequence of the complete coding region of the H5 subtype avian influenza virus HA gene is replaced by the HA1 sequence of the H7 subtype avian influenza virus HA gene, and then the substituted sequence ( Containing H5 subtype influenza virus HA gene signal peptide coding sequence, HA1 sequence, cleavage site sequence, and H7 subtype avian influenza virus HA gene HA1 sequence) replacing the H5 subtype avian influenza virus HA gene partial sequence in Example 1, Inserted between the P gene and the M gene in the genome of avian paramyxovirus type 2. The manufacturing steps of the artificial avian paramyxovirus described in Example 2 are the same as those in Example 1. The virus is used as a live vaccine prepared from influenza vaccine strains, which can be used to prevent the attack of a...

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Abstract

The invention belongs to the field of biological medicine and discloses an artificial avian paramyxovirus. The genome of the artificial virus contains a backbone sequence (which is the genomic sequence of a certain avian paramyxovirus) and a partial sequence of an influenza virus hemagglutinin gene (which does not encode a polypeptide anchored to the viral envelope). Unlike previously similar artificial avian paramyxoviruses, the artificial avian paramyxovirus does not alter their capsular structure. Therefore, if the artificial virus is used as an influenza vaccine (being a live vaccine) strain for humans and animals, it can be ensured that the artificial virus normally proliferates in an inoculated animal in terms of the structure of the viral envelope, thereby ensuring its immune effect. In addition to inserting partial sequences of influenza virus genes into the genome of the artificial virus, it is also possible to insert a protein coding sequence of another class of viruses, so that an animal inoculated with the artificial virus also produces a specific immune response against another type of viruses.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to an artificial animal virus, which can be used as an influenza vaccine strain for humans or animals. Background technique [0002] Before the declaration of the present invention, the strains used for human or animal influenza vaccines were divided into natural viruses (that is, non-artificial viruses), viruses that were modified based on natural viruses, and viruses that were not based on natural viruses. Man-made viruses. Among them, artificial viruses include artificial avian influenza virus, artificial avian paramyxovirus, and other artificial viruses. Both artificial avian influenza virus and artificial avian paramyxovirus have been used in the prevention and control of H5 subtype avian influenza in China. Among them, the artificial avian paramyxovirus is used as a live vaccine for poultry to prevent H5 subtype avian influenza. These live vaccines multiply in the vaccinat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01
Inventor 陈继明靳继惠
Owner CHINA ANIMAL HEALTH & EPIDEMIOLOGY CENT
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