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Anti-Hib-RSV-meningococcus combination vaccine

A meningococcal and combined vaccine technology, applied in vaccines, antibacterial drugs, multivalent vaccines, etc., can solve the problems of low expression level of fHbp protein, and achieve the effects of high immune efficacy, simple production process and good safety.

Inactive Publication Date: 2018-10-09
BRAVOVAX
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Problems solved by technology

[0015] However, the use of fHbp protein alone also has certain defects, because the expression level of fHbp protein is low in some MenB strains, and a vaccine with only fHbp antigens cannot be used for a wide range of immune meningococcal infections. Fusion expression of multiple antigens may well be a good choice

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  • Anti-Hib-RSV-meningococcus combination vaccine
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  • Anti-Hib-RSV-meningococcus combination vaccine

Examples

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Embodiment Construction

[0046] The anti-Hib-RSV-meningococcal combined vaccine provided by the present invention will be further described in detail and completely below in conjunction with the examples. The embodiments described below are exemplary only for explaining the present invention and should not be construed as limiting the present invention.

[0047] The experimental methods in the following examples are conventional methods unless otherwise specified. The experimental materials used in the following examples were purchased from the market unless otherwise specified.

[0048] 1. Preparation of Hib-meningitis joint immune intermediate

[0049] 1.1. Cloning and prokaryotic expression of ΔfHbp and ΔfHbp-NadA recombinant proteins

[0050]fHbp is a membrane surface lipoprotein, and many strains of meningococcus have its gene, and strains that do not carry the gene have also been found. According to the antigenic cross-reactivity and sequence similarity of the whole protein, meningococcal fHb...

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Abstract

The invention discloses an anti-Hib-RSV-meningococcus combination vaccine comprising a Hib-meningococcus intermediate and an RSV immune intermediate, wherein the meningococcus immune intermediate comprises modified delta fHbp protein, a flexible linker peptide segment and NadA protein, the recombinant delta fHbp protein comprises VA and VB domains of fHbp variant V1 and VC, VD and VE domains of fHbp variant V3, and Hib capsular polysaccharides are conjugated to the delta fHbp protein and / or the NadA protein after being activated; the antigen of the RSV immune intermediate is an RSV membrane surface fusion protein F and / or attachment protein G which can cause body immune response, the nucleic acid sequence expressing the protein antigen is recombined on a nucleic acid vector, the nucleic acid sequence of the recombinant protein takes attenuated intracellular bacteria as a bacteria vector; the immune intermediates of the components are separately prepared and then mixed for use.

Description

technical field [0001] The invention relates to an anti-Hib-RSV-meningococcus combined vaccine, which belongs to the field of biopharmaceuticals. Background technique [0002] 1. Haemophilus influenzae and its epidemiology [0003] Haemophilus influenzae type b is the most invasive serotype of Haemophilus influenzae, and it is an important pathogenic bacterium that causes severe infections in children. It mainly infects children under the age of 5, especially infants and young children under the age of 2. Hib spreads through the respiratory tract of susceptible individuals and colonizes the nasopharynx. It can be asymptomatically carried for several months, and a small number of people develop Hib invasive disease. The most common diseases of Hib infection are meningitis and pneumonia, and also include osteomyelitis, septic arthritis, epiglottitis and sepsis. The most important virulence factor of Hib is its capsular polysaccharide PRP, which can provide survival advantage...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61K39/095A61K39/102A61K39/155A61P31/04A61P31/14
CPCA61K39/095A61K39/102A61K39/12A61K2039/70A61P31/04A61P31/14C12N2760/18534
Inventor 吴克闫利明刘昊智孙晓东
Owner BRAVOVAX
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