14 results about "Haemophilus" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to an oral microorganism for identifying taste sensitivity as well as an identification method and application of the oral microorganism. On the basis of comparative study of young and old population, a screened oral microorganism combination covers wide characteristics of taste sensitivity difference, and the abundance of one or more of five oral microorganisms of a Haemophilus genus, a Fusobacterium genus, an Aggregate genus, a Lacnoanaerobacter genus and an Oribacterium genus is in significant positive correlation with the taste sensitivity, so that the oral microorganism combination can be used for accurately identifying the oral microorganisms of one or more of the five oral microorganisms of the Haemophilus genus, the Fusobacterium genus, the Aggregate genus, the Lacnoanaerobacter genus and the Oribacterium genus.

The invention belongs to the technical field of biology, and particularly relates to a quintuple fluorescent PCR detection reagent for porcine respiratory pathogens and application of the quintuple fluorescent PCR detection reagent. The quintuple fluorescent PCR detection reagent disclosed by the invention can achieve the purpose of simultaneously identifying and detecting actinobacillus pleuropneumoniae, haemophilus parasuis, mycoplasma hyopneumoniae, pasteurella multocida and / or streptococcus suis, and has the advantages of simplicity, convenience and rapidness in operation, high accuracy, high sensitivity and good repeatability; the method has important significance on rapid and accurate detection and epidemic disease prevention and control of clinical porcine respiratory pathogens.

The invention relates to the technical field of biological detection, in particular to a nucleic acid combination product, a PCR premix, a method and a kit for identifying respiratory tract pathogenic bacteria. The nucleic acid combination product comprises a primer pair as shown in SEQ ID NO: 1 to SEQ ID NO: 21 and a probe. The invention provides a nucleic acid detection product for detecting klebsiella pneumoniae, streptococcus pneumoniae, haemophilus influenzae, pseudomonas aeruginosa, acinetobacter baumannii, staphylococcus aureus and moraxella catarrhalis, and the nucleic acid detection product can be used for performing amplification detection on conserved fragments of target pathogens in the same PCR reaction system, and is efficient and low in cost. Moreover, the anti-interference capability is strong, and the sensitivity is high.

The invention relates to the technical field of molecular diagnosis and early screening of tumors, and particularly discloses a plasma circulating microbial marker for early screening of lung cancer and application of the plasma circulating microbial marker. The marker comprises the following components: staphylococcus aureus, fusobacterium nucleatum, klebsiella pneumoniae, alternaria simplex, legionella pneumophila, neisseria mucosa, Ralstonia petii, pasteurella multocida, and haemophilus parainfluenza (Haemophilus parainfluenza), and the marker comprises the following components: staphylococcus aureus, fusobacterium nucleatum, klebsiella pneumoniae, alternaria simplex, legionella pneumophila, neisseria mucosa, Ralstonia petii, pasteurella multocida, and Haemophilus parainfluenza (Haemophilus parainfluenza). According to the method, nine characteristic microbial markers are detected in combination with a low Pass WGS technology, a high-precision diagnosis model is constructed through a machine learning algorithm, the established diagnosis model has excellent stability and clinical applicability, efficient recognition of early lung cancer can be achieved, and the detection cost can be greatly reduced.

The present invention belongs to the technical field of bacterial infection assessment, and specifically provides a method for assessing the infectivity of Haemophilus influenzae, comprising the following steps: (1) constructing nasal epithelial organoids; (2) incubating nasal epithelial organoids with Haemophilus influenzae to be tested, obtaining infected nasal epithelial organoids, and conducting subsequent infection status assessment; (3) infection status assessment: comparing the mRNA expression levels of TNFa, IL6, IL8, DNAH5, RSPH1, and GAS8 in nasal epithelial organoid cells before and after Haemophilus influenzae infection; when the expression of TNFa and IL8 in the infected cells increases, and the expression of IL6, DNAH5, RSPH1, and GAS8 decreases, the Haemophilus influenzae to be tested has respiratory tract infectivity, thereby quickly assessing the infectivity and destructiveness of Haemophilus influenzae from patient samples, and providing an important research and application platform for the high-throughput screening and development of Haemophilus influenzae pathogenicity and antibacterial drugs.

Provided are a method for enhancing the immunogenicity of a recombinant protein antigen and use thereof. The method comprises conjugating a polysaccharide with a recombinant protein antigen to form a nano-scale protein antigen. The polysaccharide is selected from sodium hyaluronate, chitosan, glucan, fucoidan, and sodium alginate. The recombinant protein antigen is a protein antigen derived from a bacterium. The bacterium belongs to the genus of Staphylococcus, Neisseria, Klebsiella, Escherichia, Clostridium, Salmonella, Shigella, Pseudomonas, Acinetobacter, Bordetella, Enterococcus, Haemophilus, Mycobacterium, or Streptococcus. The method not only improves the immunogenicity of the recombinant protein antigen, but also overcomes the defects of competitive immunoregulation and poor immune enhancement effects caused by bacterial capsular polysaccharide modification. The method also has the advantages of high clinical application value, simple starting material acquisition, low cost, good process stability, ease in scale expansion, high safety, and the like.

This invention belongs to the field of microbial technology, specifically relating to an oral microorganism for identifying taste sensitivity, its identification method, and its application. The microorganisms include... Haemophilus genus, Fusobacterium genus, Aggregatibacter genus, Lachnoanaerobaculum genus and Oribacterium One or more of the above-mentioned microorganisms are identified, and the abundance of one or more of these microorganisms is significantly positively correlated with taste sensitivity. The application method is as follows: 1) Collect oral biological samples; 2) Extract DNA from saliva samples and perform sequencing analysis; 3) Detect the abundance of microbial assemblage; 4) Verify the correlation between key characteristic oral microorganisms and individual taste sensitivity. The advantage is that it is a non-invasive and non-surgical method to determine individual differences in taste sensitivity, aiming to achieve efficient, low-cost, and accurate identification of individual taste sensitivity.

The invention discloses application of oral microorganisms in diagnosis of severe depressive disorder with sleep disorder, and belongs to the field of microbiomics and mental disease diagnosis. Specifically, the application comprises the application of the oral microorganism detection reagent in the preparation of the kit for diagnosing the severe depressive disorder with the sleep disorder, and the oral microorganisms comprise Solobacterium, Granulicatela, Campylobacter and Haemophilus. The invention further discloses the application of the oral microorganism detection reagent in the preparation of the kit for diagnosing the severe depressive disorder with the sleep disorder, and the oral microorganisms comprise Solobacterium, Granulicatela, Campylobacter and Haemophilus. Particularly, the Haemophilus has relatively high specificity and relatively excellent performance of a working characteristic curve (ROC) of a subject in diagnosis. The relative abundance of the bacteria is used as a biomarker for auxiliary diagnosis of MDD, and the capability of distinguishing depression from healthy people can be remarkably improved. The invention provides a new microecological approach for rapid and non-invasive diagnosis of depression diagnosis.

The present application relates to a kind of essential oil composition with the efficacy of improving immunity, belong to daily chemical technology field.The essential oil composition with the efficacy of improving immunity of the present application includes the following mass parts of components:Rowensha leaf essential oil 10-20 parts, eucalyptus essential oil 15-30 parts, green flower of guettarda speciosa essential oil 10-20 parts, guaiac essential oil 5-10 parts, cedar essential oil 5-20 parts.The essential oil composition with the efficacy of improving immunity of the present application can significantly reduce the secretion of TNF-alpha, and have certain inhibitory effect on streptococcus pneumoniae, haemophilus influenzae, in addition, also have certain antitussive efficacy, thereby verifying its efficacy of improving immunity, relieving respiratory discomfort.The composition of the present application can be used for preparing massage oil, aromatherapy spray, inhalant, cream, patch and other care products, with good application prospect.

The invention relates to an autoimmune hepatitis related intestinal flora detection kit and a detection method, and the kit comprises six primer probe groups for detecting autoimmune hepatitis intestinal flora, the six primer probe groups are respectively used for detecting at least one of streptococcus, faecalis, colabacillus, megamonas, haemophilus and butyric acid-producing enterobacter, and the six primer probe groups are respectively used for detecting at least one of streptococcus, faecalis, colabacillus, megamonas, haemophilus and butyric acid-producing enterobacter. By adopting the technical scheme, the specific primers and probes aiming at streptococcus, colabacillus, haemophilus, megamonas, faecalis and butyric acid producing enterobacter are designed, so that the kit has the characteristics of high specificity, high sensitivity, rapidness and high efficiency, can accurately detect the change of intestinal flora of a patient, and can be used for detecting the intestinal flora of the patient. And a reliable microbial marker is provided for the diagnosis of the autoimmune hepatitis.

The present application relates to the field of ophthalmic drugs, and particularly relates to an ophthalmic composition, a preparation method and application thereof, the ophthalmic composition comprising a compound as shown in formula I, an osmotic pressure regulator, a pH regulator and water for injection; the pH value of the ophthalmic composition is 4.0-6.0; the concentration of the compound as shown in formula I is 0.1-3.0% w / v, and the concentration of the osmotic pressure regulator is 0.3-10.0% w / v; the antibacterial activity against Streptococcus pneumoniae, Staphylococcus aureus and Staphylococcus epidermidis is 2-8 times that of levofloxacin and moxifloxacin hydrochloride; the antibacterial activity against Haemophilus influenzae, Moraxella catarrhalis, Escherichia coli and Pseudomonas aeruginosa is also strong, and is equivalent to that of levofloxacin and moxifloxacin hydrochloride; the irritation is small, the light stability and high-temperature stability are good, the quality and drug safety are guaranteed, and the application value is good.

The invention provides a reagent and a kit for detecting nucleic acid of canine mycoplasma haemophilus and application of the reagent and the kit. The reagent comprises a reagent for detecting a first region of the mycoplasma canine haemophilus, and the first region is selected from any fragment in SEQ ID NO: 1. By applying the reagent or the kit provided by the invention, the identification of the haemophilus canis mycoplasma can be accurately, quickly and sensitively realized.

The invention discloses a Chinese grass tortoise source Hafnia alvei strain and application thereof, and relates to the technical field of microorganism application. The virulence gene combination is derived from liver tissues of diseased Chinese grass turtles, the whole genome of the virulence gene combination comprises a phenol glycolipid system gene cluster, a multiple iron acquisition system and type VI secretion system gene cluster, an adhesin array and toxin system gene cluster and an alginate biofilm and multiple secretion system gene cluster, the Hafnia alvei strain is named as Hafnia alvei JX2025 strain, and is preserved in the China Center for Type Culture Collection (Wuhan) on November 17, 2025, and the preservation number is CCTCC M 20252572. The JX2025 strain can be used as a pathogen for preparing a hafnia alvei infected animal model, and the unique virulence gene spectrum of the JX2025 strain provides important molecular basis and data support for pathogenic mechanism research of related diseases, vaccine target screening and development of novel antibacterial drugs.

A compound with broad-spectrum antibacterial activity and its antibacterial composition are provided. The compound with broad-spectrum antibacterial activity has formula (I), which are antibacterial drug source compounds with a broad antibacterial spectrum, good antibacterial activity, suitable in vivo pharmacokinetics, and significant in vivo therapeutic effects. Preferred compound BAB159 has good antibacterial activity against Staphylococcus, Clostridium perfringens, Enterococcus, Bacillus, Streptococcus, Haemophilus, Candida krusei, Aspergillus niger, and Trichophyton, which is comparable or better than the listed drugs. The combination of preferred compound BAB159 and polymyxin E exhibits significantly enhanced synergistic antibacterial activity, especially against multidrug-resistant strains.