52 results about "Human Parainfluenza Virus" patented technology

The invention provides biologically active compounds that may be reacted with macromolecules, such as albumin, to form covalent linked complexes wherein the resulting complexes exhibit a desired biological activity in vivo. More specifically, the complexes are isolated complexes comprising a biologically active moiety covalently bound to a linking group and a protein. The complexes are prepared by conjugating a biologically active moiety, for example, a renin inhibitor or a viral fusion inhibitor peptide, with purified and isolated protein. The complexes have extended lifetimes in the bloodstream as compared to the unconjugated molecule, and exhibit biological activity for extended periods of time as compared to the unconjugated molecule. The invention also provides anti-viral compounds that are inhibitors of viral infection and / or exhibit anti-fusiogenic properties. In particular, this invention provides compounds having inhibiting activity against viruses such as human immunodeficiency virus (HIV), respiratory syncytial virus (RSV), human parainfluenza virus (HPV), measles virus (MeV), and simian immunodeficiency virus (SIV) and that have extended duration of action for the treatment of viral infections.

The present disclosure relates to novel compounds of formulas (1) through (19) and to a method for treating humans infected with a virus including various respiratory viruses such as members of the Paramyxoviridae family (respiratory syncytial virus (RSV), human metapneumovirus (HMPV), human parainfluenza virus (HPIV), measles virus, and mumps virus) with a compound of formulas (1) through (19). The present disclosure also relates to a cytopathic effect (CPE)-based assay that will assess virus-induced CPE for screening of compounds for treating viral diseases or inhibiting a virus.

The present invention relates to C34 peptide derivatives that are inhibitors of viral infection and / or exhibit antifusogenic properties. In particular, this invention relates to C34 derivatives having inhibiting activity against human immunodeficiency virus (HIV), respiratory syncytial virus (RSV), human parainfluenza virus (HPV), measles virus (MeV), and simian immunodeficiency virus (SIV) with long duration of action for the treatment of the respective viral infections.

Recombinant human parainfluenza virus type 1 (HPIV1) compostions, formulations and methods are provided. The recombinant HPIV1 viruses and HPIV1 chimeric and chimeric vector viruses provided according to the invention are infectious and attenuated in permissive mammalian subjects, including humans, and are useful in immunogenic composition s for eliciting an immune responses against one or more PIVs, against one or more non-PIV pathogens, or against a PIV and a non-PIV pathogen. Also provided are isolated polynucleotide molecules and vectors incorporating a recombinant HPIV1 genome or antigenome.

The invention provides a kit for jointly detecting respiratory tract pathogen through a multiple fluorescent PCR method. The kit comprises six components: reaction liquid A, reaction liquid B, reaction liquid C, enzyme mixed liquid, positive control and negative control, and comprises 11 common respiratory tract pathogen detections (general type of influenza virus A, influenza virus B, respiratory syncytial virus, 1 / 2 / 3 type of human parainfluenza virus, adenovirus, mycoplasma pneumoniae, chlamydia pneumonia, legionella pneumophila, streptococcus pneumonia, haemophilus influenza, A streptococcal); the amplification is performed through three reaction buffers, and each reaction buffer contains four fluorescent channels, 90% pathogen infection on the clinic can be checked.

The invention discloses a Multiplex PCR detection kit for nucleic acids of twelve respiratory viruses. The kit includes primers for amplifying the specific gene loci of the following twelve respiratory viruses: influenza A virus (Inf A), influenza A H1N1 (2009), influenza A H3N2, human parainfluenza virus (HPIV1), human parainfluenza virus (HPIV2), human parainfluenza virus (HPIV3), human parainfluenza virus (HPIV4), human metapneumovirus (hMPV), respiratory adenovirus (AdV), respiratory syncytial virus (RSV), bocavirus (BoV) and coronavirus (CoV). The kit allows multiplex detection, has high sensitivity and is convenient and quick to use. Specific primer sequences ensure reliability of detection results. A detection method is simple in operation, save time and labor intensity, has high detection throughput, is low in cost of reagent and consumable, and can directly detect the nucleic acids extracted from a respiratory pathogen sample, is low in requirement on detection platform and operation technology, and can be widely promoted in common detection.

The invention discloses a nucleic acid combined testing kit of respiratory tract infection pathogens. The invention develops a set of primer-probe combinations which can detect multiple types of respiratory tract infection pathogens such as novel coronavirus, influenza virus a, influenza virus b, respiratory syncytial virus, human parainfluenza virus, adenovirus, mycoplasma pneumonia and chlamydiapneumonia through combination of a multiple fluorescence quantitative PCR technology and a flow-through hybridization and gene chip technology, wherein nucleotide sequences thereof are shown by SEQ ID NO:1-36 respectively. The nucleic acid combined testing kit of the respiratory tract infection pathogens is established. The kid can realize synchronous combined testing of the 8 respiratory tract infection pathogens, is high in detection accuracy, specificity and sensitivity, good in repeatability, low in false negativity and false positivity, short in detection time and low in cost, can realize comprehensive detection of a patient, can locate a disease source accurately, can realize treatment in time or make corresponding quarantine measures and is of important significance to effective control of respiratory tract infection and subsequent prevention of outbreak of relevant contagion and infection.

This invention relates to C34 peptide derivatives having improved aqueous solubility that are inhibitors of viral infection and / or exhibit antifusogenic properties. In particular, this invention relates to C34 derivatives having inhibiting activity against human immunodeficiency virus (HIV), respiratory synctial virus (RSV), human parainfluenza virus (HPV), measles virus (MeV), and simian immunodeficiency virus (SIV) with long duration of action for the treatment of the respective viral infections.

The invention relates to probes and assays which are used for the simultaneous detection, in a single assay sample, of a plurality of nucleic acid sequences of viruses that cause respiratory infections in humans, selected from among influenza virus type A, influenza virus type B, influenza virus type C, human respiratory syncytial virus type A, human respiratory syncytial virus type B, human adenovirus, human parainfluenza virus type 1, human parainfluenza virus type 2, human parainfluenza virus type 3, human parainfluenza virus types 4A and 4B, enterovirus, rhinovirus, human coronavirus type 229E, human coronavirus type OC43, coronavirus that causes severe acute respiratory syndrome (SARS), human metapneumovirus and combinations thereof.

The invention relates to three reagent kits for detecting human parainfluenza viruses 1, 2, 3 type real-time fluorescence polymerase chain reaction, which respectively adopts a one-step method RT-PCR, a two-step method RT-PCR and a multicolor fluorescence method RT-PCR to distinguish types of the human parainfluenza viruses of multi-type specimens and fix an amount of the human parainfluenza viruses of the multi-type specimens. The detection methods of the reagent kits have simple operation, short consuming time and high sensitivity and specificity and can be extensively used in a plurality of fields, such as the auxiliary diagnosis of the infection of the human parainfluenza viruses, clinical medicine direction, epidemiology retrospective study, and the like.

The invention discloses a kit for simultaneously detecting nucleic acids of seven respiratory pathogens and application of the kit. The kit is based on a double amplification technology (RNA isothermal amplification and multi-biotin signal amplification), and can detect H1N1 / H3N2 type influenza A viruses, influenza B viruses, respiratory syncytial viruses, 1 / 2 / 3 type human parainfluenza viruses, B / E type adenoviruses, mycoplasma pneumoniae and chlamydia pneumoniae. The kit of the invention does not need RNA extraction, and is not prone to pollution, high in sensitivity and good in specificityin the process of detection, and can be widely applied to detection of the nucleic acids of the above seven respiratory pathogens.

The invention discloses a respiratory tract infection pathogen nucleic acid combined detection kit. The invention develops a primer and probe combination for detecting various respiratory tract infection pathogens such as novel corona virus, influenza A virus, influenza B virus, respiratory syncytial virus, human parainfluenza virus, adenovirus, mycoplasma pneumoniae and chlamydia pneumoniae by combining a multiple fluorescent quantitative PCR technology and a diversion hybridization gene chip technology. The nucleotide sequences of the primer and probe combination are shown in SEQ ID NO: 1-36in sequence. The respiratory tract infection pathogen nucleic acid combined detection kit is constructed. Synchronous joint detection of eight respiratory tract infection pathogens can be realized, the detection accuracy is good, the specificity is strong, the sensitivity is high, the repeatability is good, false negative and false positive are low, the detection time is short, the cost is low, apatient can be comprehensively detected, the pathogens can be accurately positioned, timely treatment is carried out or corresponding isolation measures are carried out, and the kit has important significance for effectively controlling respiratory tract infection to prevent related infectious infection outbreak.

The invention provides a single-tube multiplex fluorescence PCR detection method for 1,2,3-type human parainfluenza viruses. In the method, a primer, the sequence of which is shown as SEQ ID NO:1-6 is adopted, and a probe the sequence of which is shown by SEQ ID NO:7-9 is also adopted. The invention also provides a single-tube multiplex fluorescence PCR detection kit for the 1,2,3-type parainfluenza viruses, comprising the primer and the probe. In the invention, the single-tube multiplex detection of the 1,2,3-type parainfluenza viruses is realized by adopting the self-designing PIV-1 / PIV-2 / PIV-3 specific primer and the Taqman probe and using an FAM / JOE / TAMRA multiplex fluorescein sign. The invention has the advantages of strong specificity, high sensitivity, rapidness, simple and convenient operation, low cost and the like, can be used as a reagent for detecting the 1,2,3-type human parainfluenza viruses and is used for scientific research and clinical application.

The invention provides isolated nucleic acids encoding recombinant genomes or antigenomes of Human Parainfluenza Viruses that are useful as vaccines. The recombinant genomes or antigenomes can be incorporated into expression vectors for production of recombinant viruses in vitro. The invention also provides recombinant Human Parainfluenza viruses having one or more mutations that attenuate replication of the virus in a host.

The invention belongs to the technical field of virus detection kit, and provides a human parainfluenza virus I, II, III, and IV-type quadruple-PCR detection kit, and a detection method thereof. The human parainfluenza virus I, II, III, and IV-type quadruple-PCR detection kit comprises human parainfluenza virus specific primers and gene probes; and in the detection method, the human parainfluenza virus I, II, III, and IV-type quadruple-PCR detection kit is used for detection. Compared with the prior art, the human parainfluenza virus I, II, III, and IV-type quadruple-PCR detection kit and the detection method possess following advantages: detection of human parainfluenza virus I, II, III, and IV types can be realized at the same time; sensitivity, specificity, and accuracy are high; and detection period is short.

The invention discloses a human parainfluenza virus nucleic acid extraction-free and rapid gene typing detection kit. The kit includes a PCR amplification reagent and a contrast reagent, the PCR amplification reagent is composed of a PCR reaction buffer solution A, an enzyme system B, and a HPIV1 / 2 / 3 / 4 primer probe mixed liquor, and the contrast reagent is mainly composed of a negative contrast and a positive contrast. The kit has advantages of direct sample amplification, fast multiple detection velocity, high sensitivity, good specificity and the like.

The invention provides a human parainfluenza virus (HPIVs) IgM antibody detection test strip and a preparation method thereof. The human parainfluenza virus (HPIVs) IgM antibody detection test strip comprises a PVC bottom plate, a sample pad, a water absorption pad, a gold pad coated with an HPIVs specificity recombinant antigen, a detection line coated with a mouse anti-human IgM polyclonal antibody, and a nitrocellulose membrane (NC membrane) coated with a quality control line of a polyclonal antibody of an anti-HPIVs recombinant antigen. The human parainfluenza virus specific antibody IgM in serum of a human body is rapidly detected by applying an immunochromatographic method so that the acute infection of human parainfluenza viruses is conveniently subjected to differential diagnosis, and the certain significance is achieved for the clinical laboratory medicine.

The invention provides a human parainfluenza virus quantum dot immunochromatography typing detection card, a preparation method and applications. The detection card comprises a base plate, a sample pad, a water absorption pad, a combination pad and a detection layer. The combination pad is coated with a mixture of rabit-anti I-type, II-type and III-type human parainfluenza virus HN protein polyclonal antibodies labeled with quantum dots respectively. The detection layer is composed of a solid phase nitrocellulose membrane with three detection lines and a quality control line. The three detection lines are coated with rabit-anti I-type, II-type and III-type human parainfluenza virus polyclonal antibodies respectively. The quality control line is coated with anti-rabit IgG. The detection layer is pasted on the base plate. The combination pad and the water absorption pad are arranged above two ends of the detection layer respectively, overlap with part of the detection layer and are pasted with the detection layer and the base plate respectively. The sample pad is arranged on the combination pad, overlaps with part of the combination pad and is pasted with the combination pad and the base plate. The provided detection card has advantages of simple operation, rapid detection, quantification, high sensitivity and the like.

The invention provides self replicating infectious recombinant paramyxoviruses. The recombinant paramyxovirus preferably have one or more attenuating mutations. In some embodiments, the recombinant paramyxovirus has a separate variant polynucleotide encoding a P protein and a separate monocistronic polynucleotide encoding a V protein. In some embodiments, recombinant paramyxovirus have at least one temperature sensitive mutation and one non-temperature sensitive mutation. Also provided are compositions and methods for using the recombinant paramyxoviruses as described herein.

The invention discloses a real-time fluorescent quantitative PCR probe primer group and a kit for rapid and qualitative typing detection of four types of human parainfluenza viruses. The kit comprises the probe primer group specially designed for the four types of human parainfluenza viruses, i.e., HPIV-1, HPIV-2, HPIV-3 and HPIV-4; primer sequences in the probe primer group are as shown in SEQ ID NO.1-27; and probe sequences in the probe primer group are as shown in SEQ ID NO. 28 to 38. According to the invention, a reaction system of the real-time fluorescent quantitative PCR kit for rapid qualitative typing detection of the four types of human parainfluenza viruses aims at conserved regions of genomes of the four types of human parainfluenza viruses and can be used for rapid detection of parainfluenza viruses in nasopharynx swabs or sputum samples, and detection results can be used for auxiliary diagnosis of symptoms such as cough, fever, pneumonia and the like causedby parainfluenza and rapidly distinguishing parainfluenza patients from other fever and pneumonia patients.

The invention relates to a fluorescence quantitative PCR (Polymerase Chain Reaction) detection method and a kit for an HPIV (Human Parainfluenza Virus). A detection primer and a probe are designed in specific to an HPIV gene sequence conserved fragment, and RNA (Ribose Nucleic Acid) of the HPIV is detected qualitatively and quantificationally by using an improved one-step method and an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) real-time fluorescence amplification technology. The method has the advantages of easiness in operating, high repeatability and high specificity and sensitivity.

This invention relates to C34 peptide derivatives having improved aqueous solubility that are inhibitors of viral infection and / or exhibit antifusogenic properties. In particular, this invention relates to C34 derivatives having inhibiting activity against human immunodeficiency virus (HIV), respiratory synctial vims (RSV), human parainfluenza virus (HPV), measles virus (MeV). and simian immunodeficiency virus (SIV) with long duration of action for the treatment of the respective viral infections.

The invention relates to the field of molecular biological detection, specifically to detection of novel coronavirus 2019-nCoV, influenza virus type A, influenza virus type B, human parainfluenza virus type I, human parainfluenza virus type II, human parainfluenza virus type III, human parainfluenza virus type IV, respiratory adenovirus, respiratory syncytial virus and human metapneumovirus. Meanwhile, the invention also provides a test kit containing the composition, an application of the composition and a method for detecting and typing respiratory tract related viruses. The composition disclosed by the invention combines a fluorescent probe method and a melting curve method, can be used for simultaneously detecting and typing the respiratory tract related viruses in one tube, is low incost, high in flux and less in time consumption, greatly improves the detection efficiency, is simple and convenient to operate, and avoids false positive result and environmental pollution caused bycross of samples through single-tube operation.

The present invention relates to isolated, attenuated viral strains of human parainfluenza virus 2 (HPIV-2), which are useful in live vaccine preparations. These strains exhibit a temperature sensitive and cold adapted phenotype useful for stimulating a protective immune response in an inoculated mammal without producing severe symptoms.

The invention relates to a human parainfluenza virus type 3 (HPIV-3) wild strain and an application thereof. The preservation number of the wild strain is CCTCC NO:V201911, the wild strain has HN protein and F protein of an HPIV-3 virus, and the wild strain can be applied to an HPIV-3 virus infected mouse model and an HPIV-3 neutralizing antibody detection experiment, and can be applied to preparation of vaccines for preventing parainfluenza type 3. The human parainfluenza virus type 3 wild strain disclosed by the invention is a cloned purified high-titer HPIV3LZ1728C19 virus strain free fromspecific exogenous factor plaque formation, and a human parainfluenza virus type 3 HN protein subunit vaccine prepared from the virus can effectively prevent parainfluenza virus type 3 infection.

The invention discloses a detection kit for HPIV (human parainfluenza virus) triple nucleic acid. The detection kit is characterized by comprising HPIV type 1, type 2 and type 3 nucleic acid PCR reaction liquid, wherein the PCR reaction liquid comprises reaction buffer liquid, deoxyribonucleoside triphosphate, upstream and downstream primers for amplification of HPIV type 1, type 2 and type 3 target polynucleotides and probes for detection of target polynucleotides, and the sequence table of the probes is shown as SEQ ID NO:1-9. The detection kit has the advantages of being rapid, convenient and simple to operate, good in detection specificity, high in sensitivity and wide in detection range. As the probes with higher specificity is applied to the kit, the kit can rapidly detect the HPIV type 1, type 2 and type 3 nucleic acids in unknown samples; reliable experimental basis is provided for diagnosing the HPIV type 1, type 2 and type 3 nucleic acids, and the technical problems of low efficiency, poor specificity and low sensitivity of existing kits are solved.

The present invention is concerned with This invention relates to C34 peptide derivatives that are inhibitors of viral infection and / or exhibit antifusogenic properties. In particular, this invention relates to C34 derivatives having inhibiting activity against human immunodeficiency virus (HIV), respiratory syncytial virus (RSV), human parainfluenza virus (HPV), measles virus (MeV), and simian immunodeficiency virus (SIV) with long duration of action for the treatment of the respective viral infections.

The invention provides self replicating infectious recombinant paramyxoviruses where the P and C genes are separated rated. The recombinant paramyxoviruses preferably have one or more attenuating mutations and / or at least one temperature sensitive mutation and one non-temperature sensitive mutation. In some embodiments, the recombinant paramyxovirus has a separate variant polynucleotide encoding a C protein and a separate monocistronic polynucleotide encoding a P protein. Also provided are compositions and methods for using the recombinant paramyxoviruses as described herein.