34 results about "Human coronavirus" patented technology

The invention discloses a primer group and a kit for simultaneously detecting nucleic acids of various respiratory pathogens based on twenty-six PCR (Polymerase Chain Reaction). The kit comprises primer pool freeze-dried powder of influenza A virus (FluA), influenza B virus (FluB), respiratory syncytial virus (RSV), human parainfluenza virus (hPIV), mycoplasma pneumoniae (MP), novel coronavirus (SARS-CoV-2), human respiratory adenovirus (HADV), human respiratory nose / enterovirus (HRV / HEV), bordetella pertussis (BP), human coronavirus (HCoV), human bocavirus (HBoV) and chlamydia pneumoniae (CP), PCR (polymerase chain reaction) freeze-dried powder and a buffer solution. According to the kit, primer system optimization is carried out on various pathogens, brand new primers and probe pools are designed, clinical sensitivity and specificity can be achieved, and meanwhile, the kit is matched with a fully-closed full-automatic detection card box integrating sample treatment and amplification and is suitable for rapid detection of respiratory tract pathogens.

Pan-coronavirus vaccines for inducing efficient, powerful and long-lasting protection against all Coronaviruses infections and diseases, comprising multiple highly conserved large sequences which may comprise one or more conserved B, CD4 and CDS T cell epitopes that help provide multiple targets for the body to develop an immune response for preventing a Coronavirus infection and / or disease. In certain embodiments, the large sequences are conserved proteins or large sequences, e.g., sequences that are highly conserved among human coronaviruses and / or animal coronaviruses (e.g., coronaviruses isolated from animals susceptible to coronavirus infections).

The application discloses an application of baicalin in treating coronavirus infectious diseases. The new application of baicalin is verified through in-vitro cell tests and mouse tests infected with human coronavirus HCoV-229E, the technical scheme is novel, and a curative effect test of baicalin in treating pneumonia caused by HCoV-229E virus infection is carried out, and the results show that baicalin has a certain inhibitory effect on HCoV-229E infected cells in vitro; baicalin shows good drug efficacy in lung index and inhibition rate of pneumonia mice infected with HCoV-229E virus, can reduce the virus load of lung tissue of pneumonia mice infected with HCoV-229E virus, increase the proportion of peripheral blood lymphocytes of mice, and reduce the content of IL-2, IL-10, TNF-alpha and IFN-beta in lung tissue of mice.

The present invention concerns compositions comprising at least one niclosamide compound, such as niclosamide or a pharmaceutically acceptable salt thereof, and at least one permeability enhancer, such as glycerol or dimethylpalmityl-ammonio propanesulfonate (PPS), and their use of such compositions as niclosamide agents. Advantageously, the niclosamide compound is capable of being transported across a Caco-2 cell membrane by at least 150% relative to the amount capable of being transported across the Caco-2 cell membrane in the absence of the permeability enhancer. Compositions, including oral dosage forms, and methods for delivering niclosamide compounds, such as for treatment or prevention of human coronavirus infections, are also provided.

The present invention concerns compositions comprising at least one niclosamide compound, such as niclosamide or a pharmaceutically acceptable salt thereof, and at least one permeability enhancer, such as glycerol or dimethylpalmityl-ammonio propanesulfonate (PPS), and their use of such compositions as niclosamide agents. Advantageously, the niclosamide compound is capable of being transported across a Caco-2 cell membrane by at least 150% relative to the amount capable of being transported across the Caco-2 cell membrane in the absence of the permeability enhancer. Compositions, including oral dosage forms, and methods for delivering niclosamide compounds, such as for treatment or prevention of human coronavirus infections, are also provided.

The present invention belongs to the field of drug therapy, and specifically relates to the use of methyl parahydroxybenzoate and its sodium salt in the preparation of drugs for treating viral infectious diseases. The present invention discloses the use of methyl parahydroxybenzoate, its sodium salt and its inhalation preparation in the treatment of coronavirus infectious diseases. By verifying the new use of methyl parahydroxybenzoate and its sodium salt inhalation solution in a mouse test infected with human coronavirus HCoV-229E, the technical solution has novelty, and an efficacy test of meta-cresol inhalation solution in treating pneumonia caused by HCoV-229E virus infection was conducted. The results showed that methyl parahydroxybenzoate and its sodium salt inhalation solution showed good efficacy in the lung index and inhibition rate of pneumonia mice infected with HCoV-229E virus, and could significantly reduce the mouse lung index.

The invention discloses an application of an eIF2alpha kinase regulator in preparation of an anti-human coronavirus drug. Specifically, the compound provided by the invention has the activity of resisting human coronavirus HCoV-OC43 and / or resisting new coronavirus of human coronavirus. The invention focuses on antiviral host factors, provides a target compound for targeting host eIF2alpha kinase, eIF2alpha phosphorylation and protein synthesis, plays an antiviral role by accurately regulating and controlling host virus interaction, and is beneficial to overcoming virus resistance and obtaining broad-spectrum antiviral activity.

At least one pharmaceutical composition for use in a method of prophylaxis or treatment of COVID wherein the at least one pharmaceutical composition comprises donepezil, rivastigmine or galantamine or one of donepezil, rivastigmine or galantamine combined with one of: Artemether and Lumefantrine; Atazanavir Sulfate; Efavirenz; Fosamprenavir Calcium; Saquinavir; Remdesivir (GS-5734); Digoxin; Memantine; Rivastigmine; Galantamine; Valsartan; Teriflunomide and wherein the method of prophylaxis or treatment of COVID or COVID-19 comprises: providing a subject in need of said prophylaxis or treatment; providing the at least one pharmaceutical composition as defined supra; wherein said components are provided together or separately; wherein said components are administered together or separately; and wherein said components are provided in a pharmaceutically acceptable diluent, adjuvant and / or excipient; and administering a pharmaceutically effective amount of said at least one pharmaceutical composition to said subject; and wherein, in the method, said subject is provided prophylaxis or treatment of COVID and COVID relates to a human coronavirus, mouse hepatitis virus (MHV), or recombinant mouse-adapted SARS-CoV (SARS-CoV MAI 5).

The application provides a pharmaceutical composition, which comprises an antiviral peptide conjugate, for the treatment or prevention of a condition or disease associated with a coronaviral infection. The coronavirus includes human coronavirus OC43, human coronavirus HKU1, human coronavirus 229E, human coronavirus NL63, Middle East respiratory syndrome-related coronavirus (MERS-CoV), SARS-CoV, SARS-CoV-2, or any variant thereof. The pharmaceutical composition is designed for, inter alia, intranasal administration.

Antimicrobial compositions include a therapeutically effective amount of neem oil, extract of the plant Hypericum spp., polyunsaturated fatty acids (PUFAs), carrageenans, and / or chlorhexidine; and (optionally) in combination with one or more pharmaceutically acceptable carriers, additives, and / or diluents. The antimicrobial compositions are effective in treating or preventing upper respiratory tract infections, preferably upper respiratory tract infections caused by human Coronaviruses and are included within methods of treating or preventing infection of the upper respiratory tract of humans by a microbial pathogen.

The invention discloses a primer probe, a method and application for detecting human coronavirus OC43. The nucleic acid sequence of the primer comprises the sequences as shown in SEQ ID NO. 7 to SEQ ID NO. 8; the nucleic acid sequence of the fluorescent probe comprises a sequence as shown in SEQ ID NO.9. The primer and the fluorescent probe for detecting the human coronavirus OC43 are creatively designed, the human coronavirus OC43 inactivated virus standard substance can be accurately detected by using the primer, the probe and the digital valuing method, the sensitivity is high, and the specificity is strong.

This invention provides a novel small-molecule allosteric protease inhibitor, 10-(4-methylphenyl)-7-phenyl-6,7,8,10-tetrahydro-5H-indeno[1,2-b]quinoline-9,11-dione, which exhibits the highest selectivity index (SI), and shows inhibition against several SARS-CoV-2 variants of concern (VOC) and multiple human coronaviruses including MERS-CoV, SARS-CoV, and HCoV-229E. Not only does it demonstrate antiviral potency against a wide spectrum of coronavirus strains and species, but it also shows drug synergism with nirmatrelvir, which could be used in combination therapy to not only treat coronavirus-caused infections and diseases, but also lower the risk of antiviral resistance.

Methods and compositions containing a phorbol ester or derivative of a phorbol ester are provided for prevention and treatment of sudden acute respiratory syndrome (SARS) coronavirus infection, including SARS-COV-2 infection and related COVID-19 disease. Also provided are methods and compositions for preventing and treating acute inflammatory conditions and related pathogenic injuries, including Acute Respiratory Distress Syndrome (ARDS) and cytokine storm syndrome (CSS) seen in severe SARS-COV-2 / COVID-19 cases.

The present invention relates to a drug comprising a polynucleotide for the prevention or treatment of SARS-CoV-2 virus infection, wherein the SARS-CoV-2 virus is not the Wuhan wild type SARS-CoV-2 virus. The present invention relates to a polynucleotide encoding an attenuated human coronavirus or fragment thereof wherein the polynucleotide comprises at least 20 one to termination codons wherein the one to termination codons are: i) codons that are different but synonymous compared to the corresponding codons in the native human coronavirus genome; and ii) a nucleotide different from the termination codon.

The invention discloses a beta-nucleoside compound for inhibiting coronavirus as well as synthesis and application thereof, the compound has a structure as shown in formula 1, # imgabs0 is used for treating human coronavirus in coronaviridae and coronavirus alpha and beta.

The invention relates to pharmaceutical product comprising a polynucleotide for use in the prevention or treatment of a SARS-CoV-2 virus infection wherein said SARS-CoV-2 virus is not a Wuhan wild-type SARS-CoV-2 virus. The polynucleotide encodes an attenuated human coronavirus or a fragment thereof, wherein the polynucleotide comprises at least 20 one-to-stop codons, wherein a one-to-stop codon is i) a different but synonymous codon compared to the corresponding codon in a natural human coronavirus genome and ii) differs by one nucleotide from a STOP codon.

The present invention concerns the use of an alpha-glucosidase glycoprotein processing inhibitor for the treatment or prevention of human coronavirus infections, such as SARS-CoV-2 or SARS-CoV-2 variant infections. Aspects of the invention include methods for treating or preventing coronavirus infection, or a symptom thereof, by administering an alpha-glucosidase glycoprotein processing inhibitor, such as castanospermine, or a pharmaceutically acceptable salt, derivative, or prodrug thereof, to a human subject; methods for inhibiting human coronavirus infection in a human cell in vitro or in vivo; pharmaceutical compositions; packaged dosage formulations; and kits for treating or preventing human coronavirus infection.

The invention discloses an expression vector, a cell and a vaccine of multivalent coronavirus recombinant protein and a preparation method of the multivalent coronavirus recombinant protein, and relates to the technical field of preparation of protein vaccines of coronavirus. A recombinant protein technical means is utilized to prepare a novel multivalent coronavirus recombinant protein, the novel multivalent coronavirus recombinant protein is prepared into a candidate vaccine, the candidate vaccine can induce an organism to generate immunoreactions aiming at different mutant strains of beta new coronavirus and alpha human coronavirus, and the generated immunoreactions are humoral immunity, cellular immunity and / or mucosal immunity. Therefore, the expression vector and the recombinant cell for expressing the novel multivalent coronavirus recombinant protein, provided by the invention, have a good application prospect. The vaccine containing the multivalent coronavirus recombinant protein has a wider coronavirus protection spectrum, the application range of the vaccine is expanded, and the vaccine has a good immunogenicity enhancing effect.

The invention belongs to the technical field of microbial preparation of compounds. The polyketone compound provided by the invention shows excellent inhibitory activity on influenza A virus (IAV), human coronavirus (OC43) and ZIKV (Zika virus). The polyketone compound provided by the invention has any chemical structural formula shown in the specification.

The invention provides a method for extracting and enriching phenylethanoid glycoside and lignan effective components from fructus forsythiae fresh leaves. The method comprises the following steps: extracting phenylethanoid glycoside and lignan compounds, enriching MCI resin components, analyzing by liquid chromatography-mass spectrometry, and identifying. The enrichment extracted by the method provided by the invention is a mixture containing phenylethanoid glycoside and lignan compounds respectively, and can be used as a medicine for resisting the human coronavirus HCoV-229E.

The invention discloses a short and small polypeptide capable of inhibiting beta human coronavirus from invading host cells in a broad-spectrum manner and a medicinal application of the short and small polypeptide. Reasonable drug design is carried out based on the universality and conservative property of the six-strand alpha-helix bundle (6-HB) structure of coronavirus spike protein in the process that beta human coronavirus enters host cells. According to the present invention, alpha helical conformation restriction is performed on an HR2 structure domain derived polypeptide in 6-HB, such that a short and small polypeptide having a broad-spectrum inhibition effect on infection of two or more beta human coronaviruses (such as SARS-CoV, MERS-CoV, SARS-CoV-2, OC43) is provided. The polypeptide has the important advantages of being high in antiviral activity, short in sequence, easy to synthesize and the like. Candidate drug reserve and technical reserve are proactively provided for research and development of emergency prevention and control drugs for new and reproduced human coronavirus infection epidemic situations in the future.

The invention discloses a 2 '-fluoro-4'-alkynyl-6-methylmercapto substituted nucleoside derivative as well as a preparation method and application thereof, the structure of the 2 '-fluoro-4'-alkynyl-6-methylmercapto substituted nucleoside derivative is shown as a general formula (xv), and X and Y are independently selected from-H, C1-10 alkyl, halogen,-CN,-N3,-OH or-NH2. Compared with the prior art, the invention provides the 2 '-fluoro-4'-alkynyl-6-methylmercapto substituted nucleoside derivative with a novel structure, and compared with a similar compound disclosed in the prior art, the 2 '-fluoro-4'-alkynyl-6-methylmercapto substituted nucleoside derivative has obviously better anti-coronavirus activity. Experimental results show that the compound disclosed by the invention can effectively inhibit human coronavirus HCoV-229E and has a good application prospect in the aspect of treating coronavirus infection. (xv).

Synthetic MVA-based vaccine compositions for preventing or treating a virus infection such as a coronavirus infection and methods of producing the vaccines. The vaccine compositions include (i) either a single DNA fragment that includes the entire genome of MVA, or two or more DNA fragments, each including a partial sequence of the genome of the MVA such that the two or more DNA fragments, when expressed in the host cell upon co-transfection, are assembled sequentially and comprise the full-length sequence of the MVA genome, and (ii) one or more DNA sequences encoding one or more human coronavirus antigens, subunits, or fragments thereof inserted in one or more insertion sites of the MVA. The antigens, subunits, or fragments thereof are expressed in the host cell upon transfection of the one or more DNA fragments.

The invention belongs to the technical field of medicines, and discloses application of baicalein in preparation of related medicines for treating human coronavirus 229E infected pneumonia. Specifically, the invention discloses application of baicalein in preparation of medicines playing a protective role in prevention and / or treatment of respiratory dysfunction, lung injury, inflammatory response, virus infection and immune disorder caused by human coronavirus 229E infected pneumonia. Pharmacological experiments prove that baicalein can improve the respiratory function in human coronavirus 229E infected pneumonia, exert anti-inflammatory, anti-virus and immunoregulation effects, and can improve pulmonary edema, thereby achieving the purpose of treatment.

The invention belongs to the technical field of medicines, and discloses application of a Dayuan drink extract in related medicines for treating human coronavirus 229E infected pneumonia. Specifically, the invention discloses application of a Dayuan drink extract in preparation of drugs for protecting respiratory dysfunction, lung injury, inflammatory response, virus infection and immune disorder caused by human coronavirus 229E infected pneumonia. Pharmacological experiments prove that the Dayuan drink extract can improve the respiratory function in human coronavirus 229E infected pneumonia, exert anti-inflammatory, antiviral and immunoregulation effects, and can improve pulmonary edema, thereby achieving the purpose of treatment.

The invention belongs to the technical field of plant extract preparations, and discloses a plant disinfectant and a preparation method thereof. The preparation method comprises the following preparation steps: (1) mixing and crushing dried liquorice, fructus forsythiae, radix isatidis, honeysuckle, dandelion, golden cypress, cyrtomium fortunei, andrographis paniculata and oldenlandia diffusa, and then adding into hot water for standing and soaking treatment; and (2) filtering the mixture soaked in the step (1), filling a percolation column with a wet solid material, and performing low-temperature percolation extraction at the temperature of 0-30 DEG C by adopting an ethanol water solution as an extraction solvent to obtain the plant disinfectant. The preparation method of the plant disinfectant is simple, the process conditions are easy to control, and the production cost is low. Through verification, good killing effects on candida albicans, staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, tortoise mycobacterium abscess subspecies, natural bacteria, human coronavirus and influenza A virus can be achieved.

The invention provides a reporter gene-carrying recombinant human coronavirus 229E genome, a reporter gene-carrying recombinant strain, a preparation method and an application, and belongs to the technical field of recombinant viruses. The invention provides a recombinant human coronavirus 229E genome, which is based on the human coronavirus 229E genome, especially performs partial substitution on an ORF4a / b coding region of the genome, and inserts a red fluorescent protein mCherry into the human coronavirus 229E genome to obtain a recombinant virus expressing red fluorescence. By using the recombinant human coronavirus 229E genome, the recombinant virus can be successfully rescued by using the recombinant human coronavirus 229E genome; by utilizing the recombinant virus, rapid, high-throughput and visual detection can be realized, and the recombinant virus has the potential of being applied to visual in-vitro antiviral drug screening tools.

The present invention belongs to the field of drug therapy, and specifically relates to the use of ethyl parahydroxybenzoate in the preparation of drugs for preventing or treating viral infectious diseases. The present invention discloses the use of ethyl parahydroxybenzoate and its inhalation preparation in the treatment of coronavirus infectious diseases. By verifying the new use of ethyl parahydroxybenzoate inhalation solution in a mouse test infected with human coronavirus HCoV-229E, the technical solution has novelty, and an efficacy test of meta-cresol inhalation solution in the treatment of pneumonia caused by HCoV-229E virus infection was conducted. The results showed that ethyl parahydroxybenzoate inhalation solution showed good efficacy in the lung index and inhibition rate of pneumonia mice infected with HCoV-229E virus, and could significantly reduce the lung index of mice.

The application provides a pharmaceutical composition, which comprises an antiviral peptide conjugate, for the treatment or prevention of a condition or disease associated with a coronaviral infection. The coronavirus includes human coronavirus OC43, human coronavirus HKU1, human coronavirus 229E, human coronavirus NL63, Middle East respiratory syndrome-related coronavirus (MERS-CoV), SARS-CoV, SARS-CoV-2, or any variant thereof. The pharmaceutical composition is designed for, inter alia, intranasal administration.

The application discloses a novel photocatalytic plate and a preparation method, application and plate filter and disinfecting method thereof, the photocatalytic plate is prepared by a solvothermal method to prepare a TiO2 doped BiOI composite material, and is combined with iron-nickel ceramics, and is loaded on a porous metal base by adopting a 'two-time glazing + segmented drying + high-temperature rapid sintering' process. The heterojunction structure formed by TiO2 and BiOI effectively widens the light response range and inhibits the electron-hole pair recombination. In combination with the full-coating structure of the plate filter, the application shows excellent activity in the disinfection of human coronavirus and influenza A virus.