156 results about "Baicalin" patented technology

The invention relates to a radix scutellariae self-assembled nanoparticle and terbinafine co-loaded nanoparticle as well as a preparation method and application thereof, and belongs to the technical field of drug nanoparticles. The preparation method comprises the following two steps: preparing scutellaria baicalensis self-assembled nanoparticles, and preparing the scutellaria baicalensis self-assembled nanoparticles and terbinafine co-loaded nanoparticles. The co-loaded nanoparticles are prepared by the preparation method. The invention also discloses application of the co-loaded nanoparticles in treatment of dermatophytosis. The invention has the following advantages: the saturation solubility of baicalin (BCN) and terbinafine (TBF) is increased; the dissolution rate of the terbinafine (TBF) is obviously improved; the antibacterial effect of the TBF is improved; hypha growth, biomass generation and spore germination of three kinds of trichophyton of trichophyton rubrum, trichophyton mentagrophytes and microsporum canis can be inhibited.

The invention relates to the technical field of cosmetics, and discloses an anti-inflammatory soothing cosmetic composition which comprises the following components in percentage by weight: 0.5%-4% of a barrier repairing component, 0.5%-3% of an immunoregulation component, 0.3%-2.5% of a nerve soothing component, 0.3%-3% of an anti-inflammatory plant compound, 1%-10% of a moisturizing soothing enhancer and the balance of auxiliary materials. According to the components, through a five-dimensional synergistic mechanism of barrier repair, immune tolerance, nerve desensitization, anti-inflammatory itching relieving, moisturizing and stability maintaining, anti-inflammatory relieving and barrier repair are remarkably enhanced; ceramide, rare ginsenoside, phytosterol ester and free fatty acid are introduced to synergistically construct a bionic liposome which is closer to the composition of human cuticle lipid, so that the transdermal absorption and stability are enhanced, and the repair efficiency is remarkably improved; the dipotassium glycyrrhizinate, the tamarix chinensis flower extract, the purslane extract, the dinoflaxanthine and the baicalin cooperate to quickly resist inflammation and relieve itching and strengthen barrier repair, so that the composition is suitable for highly sensitive skin.

The invention is applicable to the technical field of biomedical materials, and provides an anti-inflammatory hemostatic composite gel microsphere and a preparation method and application thereof, and the preparation method comprises the following steps: mixing sodium alginate and baicalin, dissolving in deionized water to obtain a mixed solution, spraying the mixed solution into a calcium chloride solution by utilizing electrospinning and electrospraying, and carrying out electrostatic spinning and electrospraying to obtain the anti-inflammatory hemostatic composite gel microsphere. Gel microspheres are formed through ionic crosslinking; washing the gel microspheres with a calcium chloride solution, and soaking the gel microspheres in a chitosan acetic acid solution to obtain chitosan-encapsulated gel microspheres; placing the gel microspheres in a copper sulfate solution, and performing low-temperature calcium-copper ion exchange to obtain baicalin / copper alginate composite gel microspheres; and filtering, washing and freeze-drying the composite gel microspheres. The electrospray technology is combined with ionic crosslinking, the size and morphology of the microspheres are accurately controlled, the antibacterial effect on escherichia coli and staphylococcus aureus is remarkable, the in-vitro blood coagulation time is shortened by 30%, the cytotoxicity is low, and good biocompatibility is achieved.

The invention belongs to the technical field of medicines, and provides application of a natural-source compound monomer medicine composition in preparation of medicines for preventing and / or treating pancreatitis. The pharmaceutical composition comprises baicalin, paeoniflorin, geniposide, wogonin, baicalein, albiflorin, tetrahydropalmatine, rhein, wogonin, aloe-emodin, saikoside A, emodin, benzoyl paeoniflorin, chlorogenic acid, costunolide, or a pharmaceutically acceptable salt thereof. Compared with the prior art, the pharmaceutical composition disclosed by the invention has the advantages that the components of the composition are definite and are all natural sources, the safety is good, the pharmaceutical effect has a remarkable synergistic effect, the administration dosage is reduced by about 200 times under the condition that the treatment effect is better than that of a compound traditional Chinese medicine, the administration mode of the compound medicine for treating pancreatitis is innovated, and the pharmaceutical composition is suitable for clinical application. A new scheme is provided for the treatment of the disease.

The invention discloses a preparation process of a Qingjin phlegm-reducing decoction material standard, an HPLC content determination method and a fingerprint spectrum establishment method aiming at the material standard, under the content determination method, the peak shapes of chromatographic peaks of all components are symmetrical, the separation effect is remarkable, and excellent stability, specificity, linear response, repeatability, precision and accuracy are shown. According to the present invention, the fingerprint characteristics are accurately recorded, the reversed-phase high performance liquid chromatography technology is adopted to successfully obtain 13 common peaks, the baicalin is adopted as the reference peak, the relative retention time and the relative peak area of the common peaks are calculated, and the reference substance comparison is further performed to identify the 8 common peaks; the established fingerprint spectrum method also has the characteristics of good chromatographic peak shape, high separation degree, stable method, excellent repeatability, high precision and the like. In conclusion, the invention provides a set of comprehensive and reliable Qingjin phlegm-reducing decoction material standard quality control reference system, and a scientific basis is provided for the overall quality evaluation of the Qingjin phlegm-reducing decoction.

The traditional Chinese medicine is prepared from twelve traditional Chinese medicinal materials including ephedra, ledebouriella cantoniensis, southwest scutellaria baicalensis, platycodon grandiflorum, semen sinapis, semen armeniacae amarae, semen lepidii, Indian kalimeris herb, astragalus membranaceus, Chinese yam, hawthorn and liquorice, has the effects of clearing away heat and relieving exterior syndrome, and is used for treating exogenous cough of children, lung-dispersing cough, lung-dispersing cough, lung-dispersing cough, lung-dispersing cough, lung-dispersing cough, lung-dispersing cough, lung-dispersing cough and lung-dispersing cough. The fingerprint spectrum established by the invention comprises 15 common characteristic peaks which respectively belong to eleven medicinal materials including semen armeniacae amarae, radix astragali seu hedysari, rhizoma dioscoreae, radix saposhnikoviae, herba ephedrae, semen lepidii, semen sinapis, radix platycodonis, southwest radix scutellariae, radix glycyrrhizae and fructus crataegi, so that the eleven medicinal materials in the traditional Chinese medicine can be identified, and meanwhile, two components in the eleven medicinal materials, namely baicalin and chlorogenic acid, are identified. The quality of the medicine can be controlled more comprehensively, the problem of fake and inferior phenomena of the medicine is solved, and the safety and effectiveness of clinical use are ensured.

The invention relates to a composite microsphere for enhancing the antibacterial performance of baicalin as well as a preparation method and application of the composite microsphere, and relates to the technical field of biological materials. The composite microsphere comprises zein and baicalin, wherein the baicalin is embedded into the hydrophobic interior of the zein. The composite microspheres can reduce the minimum inhibitory concentration of the baicalin and the cell membrane conductivity of the baicalin, and enhance the biological membrane clearance rate of the baicalin and the DNA inhibition effect of the baicalin, so that the antibacterial property of the baicalin is improved, and the composite microspheres have wide application prospects in the industries of biological materials and the like.

The invention provides a quality detection method of antiviral particles. The quality detection method adopts HPLC-MS / MS (High Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) to simultaneously determine the following components: forsythiaside A, patchouli ketone, verbascoside, mangiferin, 5-methoxy-8-hydroxypsoralen, curcumenol, luteolin-7-glucuronide, kaempferol-3-O-rutinoside, baicalin, rutin and bergamot phenol. The detection method is a quantitative determination method for 11 active components in the antiviral particles, the detection method is short in time and low in cost, the quantitative analysis method is expected to provide reference for quality control of the antiviral particles, and the clinical curative effect and safety of the antiviral particles are guaranteed.

A composite membrane comprises a membrane matrix formed by the stable cross-linking of silk fibroin with β-sheets, and baicalin uniformly dispersed between the segments of the silk fibroin molecular chain, wherein the mass ratio of silk fibroin to baicalin is 100:3-4. The preparation method includes the following steps: 1) taking silkworm cocoons, boiling them several times with sodium carbonate solution, washing them, and drying them to 60°C; 2) dissolving them in a ternary solution, centrifuging and filtering to obtain a clear silk fibroin solution, and drying it to obtain silk fibroin; 3) preparing a silk fibroin solution with a concentration of 1-30 mg / ml, and adjusting the pH to 7.4; 4) adding baicalin to the silk fibroin solution, forming a film, drying it, and annealing it to obtain the composite membrane. The composite membrane of this invention has superior biocompatibility, hemostatic properties, and controllable biodegradability, and can promote M2 polarization of macrophages, shorten the inflammatory period, and effectively promote skin wound healing.

The invention discloses a metal organic framework compound, a preparation method and application in environmental pollutant detection. The metal organic framework compound can be used for detecting environmental pollutants such as baicalin, Fe < 3 + > and CrO4 < 2-> in water. The novel metal organic framework compound material synthesized by the invention has excellent luminescence property, not only can identify heavy metal ion Fe < 3 + > in numerous metal ions, but also can identify anion CrO4 < 2-> with strong pollution property, and meanwhile, can identify baicalin molecules in various antibacterial drug molecules in water. The material has low detection limit on different ions and molecules, and has the advantages of convenience in preparation, low cost, simplicity in operation, high sensitivity and high selectivity.

The invention relates to the technical field of microbial probiotic preparations, in particular to a sodium alginate multifunctional composite microsphere as well as a preparation method and application thereof. The sodium alginate multifunctional composite microspheres are obtained by dissolving the carbohydrate vesicles, the traditional Chinese medicine nanoparticles and the probiotics in a sodium alginate solution and then dropwise adding the solution into a calcium chloride solution through an ionic cross-linking effect. According to the invention, the amphiphilic xylooligosaccharide-cystamine derivative with H2S response is synthesized through a click reaction on the basis of prebiotic xylooligosaccharide. And the amphipathic saccharide vesicles are obtained through self-assembly by virtue of hydrophilic and hydrophobic effects. Sanguinarine iodide prepared by purification, separation and reflux and baicalin are self-assembled in an aqueous solution through a non-covalent bond, and the traditional Chinese medicine nanoparticles are prepared through ultrasonic treatment. An in-vitro simulated release experiment proves that the sodium alginate multifunctional composite microsphere is relatively low in release rate under an acidic condition, so that substances are favorably delivered to intestinal tracts; and the release of encapsulated substances can be better realized in neutral and alkaline environments.

This invention relates to diselenylene-bridged mesoporous silica nanoparticles loaded with baicalin and their applications. The invention constructs a ROS-responsive, microglia-inspired Ba@Se-MSN&BV2 nanoplatform targeting the secondary phase of spinal cord injury. The BV2 cell membrane-mediated enrichment at the injury site, combined with the diselenylene-triggered, on-demand release of baicalin, enables targeted intervention in a highly oxidative and pro-inflammatory microenvironment. In vitro and in vivo studies show that Ba@Se-MSN&BV2 can alleviate oxidative stress and mitochondrial dysfunction, inhibit apoptosis, promote axonal and neuronal survival, and improve motor circuit function. This invention provides a new therapeutic target and research direction for neuroinflammatory injuries driven by redox homeostasis disruption, with significant clinical application prospects and social value.

The application discloses a baicalin ternary co-amorphous drug and a preparation method and application thereof, and the amorphous substance can effectively improve the solubility and dissolution of puerarin and baicalin. The application mixes metformin hydrochloride, puerarin and baicalin according to a molar ratio of 1:1:1, and obtains the baicalin ternary co-amorphous drug through a ball milling method or a solvent-assisted milling method, the preparation method is green and efficient, the co-amorphous substance has high light stability and thermal stability, the solubility is increased by 1.7 times compared with that of a puerarin raw drug crystal, the solubility is increased by 46.8 times compared with that of a baicalin raw drug crystal, and the solubility is reduced by 14.8 times compared with that of a metformin hydrochloride raw drug crystal. The baicalin ternary co-amorphous drug has certain application prospects in liver protection, blood sugar reduction, treatment of damp-heat type colitis and cervical cancer.

The application discloses a preparation method of a hydrocolloid dressing for oral cavity, and relates to the technical field of medical supplies. Baicalin extract and a polyvinyl pyrrolidone solution are mixed to obtain a baicalin extract solution; chitosan fibers, hydroxymethyl cellulose and the baicalin extract solution are mixed to obtain a mixed solution; vacuum degassing: the prepared mixed solution is vacuumized, pressure is maintained, and primary degassing is performed; ultrasonic degassing: the mixed solution after primary degassing is subjected to ultrasonic degassing; static degassing: the solution after ultrasonic degassing is sealed by using a preservative film, and is subjected to secondary degassing at room temperature; a PU film is fixed in a film laying frame, the degassed mixed solution is poured into the film laying frame, and the film laying frame is placed into an air-blowing drying box to solidify and form a protective layer, so that the hydrocolloid dressing is obtained. The hydrocolloid dressing prepared by the method has good adhesion, can be used on a wound for a long time, and can effectively promote the repair of damaged cells and wounds, so that the healing of the wound in the oral cavity is promoted.

This invention belongs to the field of baicalin crystallization technology, and relates to a leaf-shaped baicalin crystal, its preparation method, and its application. The crystal has a leaf-like microstructure with a length ranging from 1 μm to 17 μm; the bulk density of the crystal is 0.1-0.2 g / mL, and the compactness is 0.2-0.4 g / mL. The leaf-shaped baicalin crystals provided by this invention are easy to compress into tablets and have a high surface area / volume ratio, which is beneficial for its adsorption and dissolution in vivo. The preparation process is simple to operate and can directionally prepare leaf-shaped baicalin crystals. The purity of the obtained leaf-shaped baicalin crystals can reach 93% or higher, significantly improving the chemical purity and forming a unique low bulk density physical property.

The present invention discloses a method for constructing a fingerprint of Gualou Niu Bang decoction, relating to the technical field of analytical chemistry. The method is simple and has high precision, stability, and repeatability. Determination revealed that seven components, including geniposide, liquiritin, hesperidin, arctiin, baicalin, melaleucalyptol A-7-O-β-D-glucuronide, and wogonin, can be used as common characteristic peaks in the fingerprint of Gualou Niu Bang decoction for identification research.

The invention provides a method for constructing a Xiaozhi decoction characteristic fingerprint spectrum based on a UPLC wavelength switching method and application thereof, and relates to the technical field of traditional Chinese medicine quality control. The fingerprint spectrum is constructed based on a UPLC (Ultra Performance Liquid Chromatography) wavelength switching method; in the construction of the fingerprint spectrum, the detection wavelength is selected as follows: the detection wavelength of 0-(Rt baicale-0. 6) min is 210 nm; the detection wavelength of (Rt baicale-0. 6)-(Rt baicalin + 0. 6) min is 360 nm; and the detection wavelength of the (Rt baicalin + 0.6)-40 minutes is 290 nm. By selecting the UPLC detection wavelength and the ultra-high performance liquid chromatography conditions, 22 common chromatographic peaks can be calibrated and 18 chromatographic peaks can be identified in 40 minutes in the characteristic fingerprint of the Xiaoshenfen decoction, more comprehensive quality evaluation can be performed on the Xiaoshenfen decoction, the time is short, and a scientific basis can be provided for quality evaluation and control of the Xiaoshenfen decoction.

To provide a skin cosmetic composition that exhibits excellent anti-inflammatory effects on the skin by increasing the production of IL-1ra, which prevents inflammatory signaling to cells. [Solution] (A) Curcumin and, (B) Contains baicalin, The skin cosmetic composition is characterized in that the mass ratio of the content of component (B) to the content of component (A) [(B) / (A)] is 0.05 or more and 5 or less.

The application aims to provide a preparation method and application of a photocrosslinking zinc-doped baicalin carbon dot-based hydrogel, and belongs to the field of biomedical technology.The baicalin and zinc acetate solution are used as precursors to prepare zinc-doped CDs at 180 DEG C.Meanwhile, a methacrylated gelatin precursor solution is prepared, the carbon dots are added in a certain proportion, and the zinc-doped baicalin carbon dot-based hydrogel is obtained under ultraviolet crosslinking.The carbon dot hydrogel combines the advantages of carbon dots and hydrogels, and has a certain application prospect in wound infection treatment.

The invention belongs to the technical field of traditional Chinese medicine preparations, and provides a pharmaceutical composition for recurrent urinary tract infection and a preparation method thereof. According to the invention, a compound extract for warming yang, promoting diuresis and activating blood and baicalin are synergistically compounded in an ion composite network formed by sodium alginate and chitosan, and are subjected to shear dispersion or homogenization treatment to form ion composite nanoparticles, and then the ion composite nanoparticles are subjected to calcium chloride cross-linking solidification and chitosan coating to prepare a multi-layer synergistic carrier design of the pellet; the dispersion uniformity, the forming strength, the pH response release and the storage stability of the ionic composite nanoparticles under the condition of high drug loading capacity are realized, and the core contradiction that the mechanical strength, the in-vivo swelling response release and the low-water-content storage and transportation stability are difficult to consider at the same time in the existing calcium alginate carrier system is solved; the method has wide clinical application value in comprehensive prevention and treatment of recurrent urinary tract infection.

The invention relates to a fingerprint construction method of a pharmaceutical composition for preventing and treating throat diseases, which comprises the following steps: weighing the pharmaceutical composition, adding the pharmaceutical composition into a 50-100% methanol solution, performing ultrasonic or reflux extraction, complementing the reduced weight, and filtering to obtain a test solution, the pharmaceutical composition being composed of scutellaria baicalensis, radix scrophulariae, platycodon grandiflorum, fructus chebulae, mint and liquorice; weighing baicalin and wogonoside, and adding the baicalin and wogonoside into a methanol solution to obtain a reference substance solution; and respectively injecting the test solution and the reference solution into a high performance liquid chromatograph, performing chromatographic analysis, recording corresponding chromatograms, and constructing the fingerprint spectrum of the pharmaceutical composition for preventing and treating throat diseases. The fingerprint spectrum constructed by the invention has characteristics, reproducibility and operability, and can be used for quality control of the pharmaceutical composition for preventing and treating throat diseases.

The invention relates to the technical field of medicines, in particular to a preparation method and application of a baicalin self-microemulsifying preparation taking ginsenoside as an effective auxiliary. The baicalin self-microemulsifying preparation is prepared by innovatively adding the natural medicine ginsenoside with various known pharmacological activities as one of the effective auxiliary agents, so that the problem of low solubility of the baicalin can be improved, and the oral availability of the baicalin is improved by 4.38 times; and compounding of two natural medicine components with definite biological activity on preparations is realized, and a basis is provided for clinical application of baicalin as a medicine.

The invention discloses a construction method and application of a scutellaria baicalensis stem and leaf fingerprint spectrum, and belongs to the technical field of medicine quality control. The method comprises the following steps: preparing standard substance solutions of scutellarin, baicalin, taxifolin and isoschaftoside; preparing a scutellaria baicalensis stem and leaf sample solution; detection is carried out under specific conditions through high performance liquid chromatography, a standard fingerprint spectrum of the stems and leaves of scutellaria baicalensis is made by taking a standard chromatographic peak as a reference, and the relative standard deviation of the relative retention time does not exceed + / -20%. The method disclosed by the invention is high in precision, good in stability and excellent in repeatability, effective separation of four flavonoid components can be realized within 35 minutes, and the quality characteristics of stems and leaves of scutellaria baicalensis are comprehensively reflected. The invention further provides application of the fingerprint spectrum in quality analysis of the scutellaria baicalensis stem and leaf medicinal material and the extract thereof, and reliable technical support is provided for resource development and quality control of the scutellaria baicalensis stem and leaf.

The invention relates to the technical field of pharmaceutical analysis, and discloses a method for simultaneously determining the contents of various components in Shuanling antidiarrheal granules based on a UPLC-QQQ-MS / MS. The contents of 18 components in the Shuanling antidiarrheal granules are simultaneously determined by using an ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometer. The 18 components comprise adenosine, gallic acid, protocatechuic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, liquiritin, ellagic acid, kaempferitrin, narirutin, hesperidin, baicalin, oroxylin A-7-O-beta-D-glucuronide, wogonin, baicalein, wogonin, pachymic acid B and pachymic acid A. The invention further discloses a preparation method of the pharmaceutical composition. Through the UPLC-QTRAP-MS / MS combined technology, the technical prejudice that single component depends on in quality control of the traditional Chinese medicine compound preparation is broken through, the key problems that multiple components in a complex matrix are difficult to separate, low-content components are difficult to detect and the like are solved, efficient, accurate and synchronous determination of 18 components is achieved, and the method is suitable for large-scale popularization and application. A new reference path is opened up for modernized quality control of the traditional Chinese medicine compound preparation.

The present invention provides a quality detection method for a Banxia Xiexin granule reference sample, comprising constructing a characteristic spectrum of the Banxia Xiexin granule reference sample and determining the contents of individual components of baicalin, glycyrrhizic acid, and berberine, wherein the content standards are limited to 37.83-70.26 mg / g for baicalin, 3.91-7.27 mg / g for glycyrrhizic acid, and 1.86-3.46 mg / g for berberine, and liquid chromatography is used for constructing the characteristic spectrum and determining the contents of individual components of baicalin, glycyrrhizic acid, and berberine; and the chromatographic conditions adopted are as follows: a chromatographic column using octadecylsilane bonded silica gel as a filler, methanol as a mobile phase A, and a 0.1% by volume formic acid solution as a mobile phase B, and gradient elution is performed as specified. The chromatographic conditions adopted in the present invention can obtain a characteristic spectrum with better separation and clearer peak tips, and can more accurately reflect and control the quality of the Banxia Xiexin granule reference sample.

The embodiment of the invention discloses hydrogel based on self-assembled nanoparticles as well as a preparation method and application of the hydrogel. On the basis of a supramolecular self-assembly technology of the Sanhuang heart-purging decoction, the active components including berberine hydrochloride, baicalin and tannic acid are assembled into nanoparticles and prepared into the hydrogel, the preparation is simple in effective components, the residence time of the effective components in the stomach can be prolonged, the effective components can play a therapeutic effect on the surface of gastric ulcer for a long time, and the curative effect is good. The component homogeneity and the preparation stability are remarkably improved while the synergistic efficacy of the compound is reserved.

The invention relates to a sun-proof real silk fabric based on modified curcumin and a preparation method of the sun-proof real silk fabric, and belongs to the technical field of dyeing and finishing. The preparation method comprises the following steps: S1, under the action of a catalyst, baicalin and dihydropyran are subjected to an etherification reaction in water, and etherified baicalin is obtained; s2, carrying out an activation reaction on the etherified baicalin and an activating agent in a solvent, and then continuously adding curcumin to carry out an esterification reaction, so as to obtain modified curcumin; s3, dissolving the modified curcumin in water to obtain finishing liquid, and soaking the real silk fabric in the finishing liquid for dyeing to obtain the sun-proof real silk fabric based on the modified curcumin. The baicalin is adopted to modify curcumin molecules, so that the problems that curcumin is weak in ultraviolet protection capability and the light fastness of the dyed real silk fabric is poor are solved, and a safe and environment-friendly solution is provided for improving the light fastness of the natural dye dyed real silk fabric.

The invention provides a construction method and a detection method of a high performance liquid fingerprint of granules for consolidating foundation and clearing away lung-heat. According to the construction method, high performance liquid fingerprint spectrums of a test solution and a reference solution of the primordial qi-consolidating and lung-heat-clearing granules are compared, so that the main effective active substances of the primordial qi-consolidating and lung-heat-clearing granules are detected. The reference substance solution is prepared from 3 '-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin-6 ''-O-xyloside, puerarin apigenin, daidzin, forsythiaside A, pinoresinol-4-O-glucopyranoside, baicalin, forsythin, wogonoside, baicalein and the like. Finally, a high-performance liquid-phase fingerprint spectrum technical standard of the primordial qi-consolidating and lung-heat-clearing granules is established, the quality of the primordial qi-consolidating and lung-heat-clearing granules can be effectively and comprehensively monitored according to the characteristics of existence of common peaks in the fingerprint spectrum, and the method has the characteristics of good stability, good repeatability and strong operability.

The present invention provides a method for constructing a fingerprint of a traditional Chinese medicine preparation for treating eczema, comprising the following steps: taking gallic acid, gentiopicroside, geniposide, paeoniflorin, ammonium glycyrrhizate, salvianolic acid B, baicalin, baicalein and wogonin reference substances to prepare a mixed reference substance solution; mixing the traditional Chinese medicine preparation for treating eczema with an organic solvent to prepare a test solution; the organic solvent is selected from one or more of methanol aqueous solution, ethanol aqueous solution and anhydrous ethanol; the mixed reference substance solution and the test solution are respectively subjected to high performance liquid chromatography determination; wherein the conditions adopted by the high performance liquid chromatography include: mobile phase A is selected from one or more of methanol and acetonitrile, and mobile phase B is selected from one or more of phosphoric acid aqueous solution, acetic acid aqueous solution and formic acid aqueous solution; and a gradient elution program is adopted.

The present invention discloses a method for identifying Ophiopogon japonicus, Forsythia suspensa and Baicalin in Qinghouyan Granules, and relates to the technical field of quality control of traditional Chinese medicine. The present invention mainly improves the method for preparing the test solution, specifically: the Ophiopogon japonicus test solution is first boiled with methanol and sodium hydroxide, and then extracted with chloroform and n-butanol; the Forsythia suspensa test solution is first boiled with calcium carbonate and water, and then evaporated with magnesium oxide, and then extracted with ethanol reflux; the Baicalin test solution is first ultrasonically treated in alkaline 50% ethanol, and then supplemented with 50% ethanol to make up for the weight loss. The present invention improves the purity and concentration of the sample to be tested by improving the preparation method of the test solution, and solves the problem that the existing technology for identifying Ophiopogon japonicus, Forsythia suspensa and Baicalin in Qinghouyan Granules has negative interference, negative tailing, and is difficult to judge.