16 results about "Wogonin" patented technology

The invention belongs to the technical field of medicines, and provides application of a natural-source compound monomer medicine composition in preparation of medicines for preventing and / or treating pancreatitis. The pharmaceutical composition comprises baicalin, paeoniflorin, geniposide, wogonin, baicalein, albiflorin, tetrahydropalmatine, rhein, wogonin, aloe-emodin, saikoside A, emodin, benzoyl paeoniflorin, chlorogenic acid, costunolide, or a pharmaceutically acceptable salt thereof. Compared with the prior art, the pharmaceutical composition disclosed by the invention has the advantages that the components of the composition are definite and are all natural sources, the safety is good, the pharmaceutical effect has a remarkable synergistic effect, the administration dosage is reduced by about 200 times under the condition that the treatment effect is better than that of a compound traditional Chinese medicine, the administration mode of the compound medicine for treating pancreatitis is innovated, and the pharmaceutical composition is suitable for clinical application. A new scheme is provided for the treatment of the disease.

The present application relates to the technical field of plant extraction, and particularly relates to a process method for jointly preparing baicalein and wogonin, comprising the following steps: (1) crushing the roots of Scutellaria baicalensis Georgi, adding boiling water to extract, and combining the extract; (2) concentrating the extract and adjusting the pH; (3) heating, cooling and adjusting the pH of the concentrated solution to obtain a lysis solution; (4) introducing the lysis solution into a resin column, eluting with purified water and an organic solvent, and concentrating the collected desorption solution; (5) adjusting the pH of the concentrated solution, filtering, drying and crushing the collected first filter cake to obtain a baicalein product; (6) adjusting the pH of the first filtrate and concentrating; (7) cooling, filtering and eluting the concentrated solution, and collecting the second filtrate and second filter cake; (8) adding purified water to the second filter cake to make a pulp, filtering, drying and collecting the third filter cake to obtain a wogonin product; and (9) spray drying the second filtrate to obtain a Scutellaria baicalensis Georgi flavone product. The above process method can simultaneously obtain high-yield baicalein, wogonin and Scutellaria baicalensis Georgi flavone products.

The invention relates to the technical field of pharmaceutical analysis, and discloses a method for simultaneously determining the contents of various components in Shuanling antidiarrheal granules based on a UPLC-QQQ-MS / MS. The contents of 18 components in the Shuanling antidiarrheal granules are simultaneously determined by using an ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometer. The 18 components comprise adenosine, gallic acid, protocatechuic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, liquiritin, ellagic acid, kaempferitrin, narirutin, hesperidin, baicalin, oroxylin A-7-O-beta-D-glucuronide, wogonin, baicalein, wogonin, pachymic acid B and pachymic acid A. The invention further discloses a preparation method of the pharmaceutical composition. Through the UPLC-QTRAP-MS / MS combined technology, the technical prejudice that single component depends on in quality control of the traditional Chinese medicine compound preparation is broken through, the key problems that multiple components in a complex matrix are difficult to separate, low-content components are difficult to detect and the like are solved, efficient, accurate and synchronous determination of 18 components is achieved, and the method is suitable for large-scale popularization and application. A new reference path is opened up for modernized quality control of the traditional Chinese medicine compound preparation.

The present invention provides a method for constructing a fingerprint of a traditional Chinese medicine preparation for treating eczema, comprising the following steps: taking gallic acid, gentiopicroside, geniposide, paeoniflorin, ammonium glycyrrhizate, salvianolic acid B, baicalin, baicalein and wogonin reference substances to prepare a mixed reference substance solution; mixing the traditional Chinese medicine preparation for treating eczema with an organic solvent to prepare a test solution; the organic solvent is selected from one or more of methanol aqueous solution, ethanol aqueous solution and anhydrous ethanol; the mixed reference substance solution and the test solution are respectively subjected to high performance liquid chromatography determination; wherein the conditions adopted by the high performance liquid chromatography include: mobile phase A is selected from one or more of methanol and acetonitrile, and mobile phase B is selected from one or more of phosphoric acid aqueous solution, acetic acid aqueous solution and formic acid aqueous solution; and a gradient elution program is adopted.

This invention discloses a differentiated fertilization method for Scutellaria baicalensis, belonging to the field of traditional Chinese medicinal herb cultivation technology. This method selects a corresponding ratio from multiple predetermined nitrogen, phosphorus, and potassium fertilizer application ratios based on the accumulation requirements of the target medicinal components, thereby achieving targeted regulation of different medicinal components. The multiple predetermined nitrogen-phosphorus-potassium ratios include a first ratio that increases baicalin, a second ratio that increases baicalein, a third ratio that increases wogonin, and a fourth ratio that increases wogonein. This invention allows for flexible selection of ratios according to production targets, achieving precise regulation of different medicinal components while simultaneously increasing Scutellaria baicalensis yield, and has good prospects for widespread application.

The invention provides a method for co-producing baicalin, baicalein and wogonin from scutellaria baicalensis extraction waste residues, which comprises the following steps: firstly, extracting the waste residues by adopting an ethanol solution, heating an extracting solution, adjusting the pH value, crystallizing to obtain a crude baicalin product, and refluxing and purifying by using ethanol to obtain the baicalin; the method comprises the following steps: respectively adjusting the pH value of mother liquor, concentrating, combining, extracting by using a water-saturated ethyl acetate-n-butyl alcohol mixed solvent, concentrating an extract phase, diluting by using ethanol, heating, adjusting the pH value, and crystallizing to obtain baicalein; and further adjusting the pH value of the residual filtrate, dealcoholizing, concentrating, cooling and crystallizing, and refining the obtained solid to obtain wogonin. According to the method disclosed by the invention, extraction, fractional precipitation and solvent extraction are combined for use, and the process conditions of each stage are optimized, so that high-purity baicalin, baicalein and wogonin are prepared from the scutellaria baicalensis extraction waste residues, and the utilization value of the waste residues is improved.

The invention provides a pharmaceutical composition for treating cholestatic liver injury, and the pharmaceutical composition contains an effective amount of Ferulic acid and Wogonin. The application creatively focuses on two key links in the cholestatic liver injury process, namely (1) bile acid excretion and (2) intestinal mucous barrier protection, a synergistic component of Ferulic acid and wogonin is provided, and the optimal ratio of Ferulic acid and wogonin is verified through an experimental system. Experiments prove that the cholestatic liver injury can be effectively improved by combined application of the Ferric acid and the Wogonin which are derived from traditional Chinese medicines; in addition, the effect of the medicine composition is obviously superior to the effect of independently using the Ferric acid or the Wogonin. Wide application prospects are realized.

The invention provides a method for detecting the contents of multiple index components of a coughing oral liquid, which comprises the following steps of: determining the contents of nine key pharmacodynamic index components, namely ephedrine hydrochloride, pseudoephedrine hydrochloride, apiose liquiritin, isoliquiritin, baicalin, huperzin A glycoside, wogonin, baicalein and wogonin; the detection method has the advantages of simplicity and convenience in operation, high stability, high pertinence and high practicability, can be used as a detection and evaluation method for production and internal quality components of the coughing oral liquid, and has a good market application prospect.

The present invention proposes a traditional Chinese medicine composition, comprising an active ingredient prepared from goat horn or antelope horn, scutellaria baicalensis, fritillaria cirrhosa, liquorice, rhubarb, gypsum, bezoar and green stone, wherein the active ingredients include, by mass, more than 1600 parts of amino acids, more than 120 parts of gallic acid, more than 130 parts of liquiritin, more than 20 parts of liquiritigenin, more than 400 parts of baicalin, more than 40 parts of melaleucoside, more than 120 parts of wogonin, more than 280 parts of glycyrrhizic acid, more than 12 parts of chrysin-7-O-β-D-glucuronic acid, more than 50 parts of aloe-emodin-8-O-β-D-glucoside, more than 30 parts of chrysophanol-1-O-β-D-glucoside, more than 45 parts of chrysophanol-8-O-β-D-glucoside, more than 260 parts of hyodeoxycholic acid and more than 150 parts of cholic acid. The composition of the present invention has antipyretic, anti-inflammatory and antitussive effects.

The present invention provides a method for determining the fingerprint and multi-component content of Xiao Chaihu Granules. Using optimized pretreatment and high-performance liquid chromatography, the method enables qualitative analysis of 18 components from three medicinal herbs in Xiao Chaihu Granules using the same chromatographic conditions. Simultaneously, it quantitatively analyzes seven chemical components in Xiao Chaihu Granules: liquiritin, baicalin, wogonin, baicalein, glycyrrhizic acid, saikosaponin B2, and saikosaponin B1. This method addresses the technical issues of Xiao Chaihu Granules' complex chemical composition, the mutual interference of component chromatographic peaks, and the difficulty in comprehensively controlling drug quality. This method allows for more effective quality control of Xiao Chaihu Granules.

The invention relates to the technical field of biological medicine, in particular to application of wogonin in preparation of a medicine for treating or inhibiting spinal cystic echinococcosis. The wogonin can accurately regulate and control the Nrf2-RTN4 signal axis and inhibit the generation of focus angiogenesis, so that the problem that the traditional medicine cannot block the key path Nrf2-RTN4-VEGF axis is solved, and the effect of treating or inhibiting the spinal cystic echinococcosis is achieved.

The invention belongs to the technical field of medicines, and discloses wogonin shown in a formula (I) and application of pharmacodynamically acceptable salts thereof in preparation of medicines for preventing, relieving and / or treating autoimmune demyelination diseases. On a mouse experimental autoimmune encephalomyelitis model, wogonin shows a good treatment effect, and can effectively prevent and treat pathological changes and disease progression, relieve disease severity and inhibit LPS-stimulated microglial BV2 cells from releasing an inflammatory mediator, thereby providing a new drug and a new structure for clinical treatment.

The present application relates to the technical field of plant extraction, and particularly relates to a process for simultaneously preparing baicalein and wogonin, which comprises the following steps: (1) crushing the roots of Scutellaria baicalensis Georgi and adding boiling water to extract, and combining the extract; (2) taking the extract, concentrating and adjusting the pH for standby; (3) taking the concentrated solution, heating, cooling, adjusting the pH, and collecting the lysate; (4) taking the lysate, filtering twice, and collecting the second filtrate; (5) taking the second filtrate, adjusting the pH, filtering, collecting the first filtrate and the first filter cake; (6) adding purified water to the first filter cake to make pulp, filtering, drying the collected second filter cake, and obtaining the baicalein product; (7) concentrating, crystallizing and filtering the first filtrate, drying the obtained third filtrate, obtaining the scutellarein product, adding purified water to the third filter cake to make pulp, filtering, drying the obtained fourth filter cake, and obtaining the wogonin product. The process can simultaneously obtain high-content baicalein, wogonin and scutellarein products.

The invention relates to the technical field of traditional Chinese medicine, in particular to a pharmaceutical composition for treating enteritis and a preparation method and application thereof. The pharmaceutical composition comprises: 30 wt% to 50 wt% of baicalin; 20 wt% to 30 wt% of wogonoside; 12 wt% to 18 wt% of scutellarin; 7 wt% to 14 wt% of baicalein; 5 wt% to 10 wt% of wogonin; 2 wt%-5 wt% of rutin; the sum of the use amounts of the components is 100%. The pharmaceutical composition provided by the invention is reasonable in formula, clear in medicinal composition and few in impurities, has obvious effects of clearing heat, resisting inflammation and relieving pain, and can obviously improve various symptoms caused by enteritis. Meanwhile, the pharmaceutical composition takes effect quickly, takes effect for a long time, is small in dosage, and is clear in medicinal material basis.

The invention relates to the technical field of fermentation engineering and medicines, and discloses a method for producing wogonin and hispidulin through synchronous transformation, which comprises the following steps: 1) preparing a fermentation culture medium; and 2) carrying out solid biotransformation. The wild schizophyllum commune is utilized to perform solid transformation on the traditional Chinese medicine scutellaria baicalensis, synchronous transformation production of wogonin and hispidulin is realized, the drug effects of the solid transformation product and schizophyllum commune polysaccharide are synergistically improved, and the solid transformation product can be directly used without extraction. The method is simple to operate and high in conversion efficiency, particularly the hispidulin is converted and synthesized for the first time, and the method has important application prospects and economic benefits in the field of medicines.

The invention provides a construction method of a high-performance liquid-phase fingerprint spectrum of Souqin capsules. The invention also provides a method for simultaneously determining the contents of six flavone components in the Souqinqin capsule by quantitative analysis of multi-components by QAMS (quantitative analysis of multi-components by QAMS). According to the present invention, the quantitative analysis of multi-components by single marker is adopted, the low-price baicalin is adopted as the alternative internal standard substance, and the relative correction factor is adopted to detect the contents of the six flavone components such as tectoridin, tectoridin A, tectoridin B, baicalin, wogonoside and tectorigenin in the Souqin capsule, such that the multi-component quantification is achieved, the method is simple, and the cost is controllable.