9 results about "Wogonin" patented technology

The present application relates to the technical field of plant extraction, and particularly relates to a process method for jointly preparing baicalein and wogonin, comprising the following steps: (1) crushing the roots of Scutellaria baicalensis Georgi, adding boiling water to extract, and combining the extract; (2) concentrating the extract and adjusting the pH; (3) heating, cooling and adjusting the pH of the concentrated solution to obtain a lysis solution; (4) introducing the lysis solution into a resin column, eluting with purified water and an organic solvent, and concentrating the collected desorption solution; (5) adjusting the pH of the concentrated solution, filtering, drying and crushing the collected first filter cake to obtain a baicalein product; (6) adjusting the pH of the first filtrate and concentrating; (7) cooling, filtering and eluting the concentrated solution, and collecting the second filtrate and second filter cake; (8) adding purified water to the second filter cake to make a pulp, filtering, drying and collecting the third filter cake to obtain a wogonin product; and (9) spray drying the second filtrate to obtain a Scutellaria baicalensis Georgi flavone product. The above process method can simultaneously obtain high-yield baicalein, wogonin and Scutellaria baicalensis Georgi flavone products.

The invention relates to the technical field of pharmaceutical analysis, and discloses a method for simultaneously determining the contents of various components in Shuanling antidiarrheal granules based on a UPLC-QQQ-MS / MS. The contents of 18 components in the Shuanling antidiarrheal granules are simultaneously determined by using an ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometer. The 18 components comprise adenosine, gallic acid, protocatechuic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, liquiritin, ellagic acid, kaempferitrin, narirutin, hesperidin, baicalin, oroxylin A-7-O-beta-D-glucuronide, wogonin, baicalein, wogonin, pachymic acid B and pachymic acid A. The invention further discloses a preparation method of the pharmaceutical composition. Through the UPLC-QTRAP-MS / MS combined technology, the technical prejudice that single component depends on in quality control of the traditional Chinese medicine compound preparation is broken through, the key problems that multiple components in a complex matrix are difficult to separate, low-content components are difficult to detect and the like are solved, efficient, accurate and synchronous determination of 18 components is achieved, and the method is suitable for large-scale popularization and application. A new reference path is opened up for modernized quality control of the traditional Chinese medicine compound preparation.

This invention discloses a differentiated fertilization method for Scutellaria baicalensis, belonging to the field of traditional Chinese medicinal herb cultivation technology. This method selects a corresponding ratio from multiple predetermined nitrogen, phosphorus, and potassium fertilizer application ratios based on the accumulation requirements of the target medicinal components, thereby achieving targeted regulation of different medicinal components. The multiple predetermined nitrogen-phosphorus-potassium ratios include a first ratio that increases baicalin, a second ratio that increases baicalein, a third ratio that increases wogonin, and a fourth ratio that increases wogonein. This invention allows for flexible selection of ratios according to production targets, achieving precise regulation of different medicinal components while simultaneously increasing Scutellaria baicalensis yield, and has good prospects for widespread application.

The invention provides a method for co-producing baicalin, baicalein and wogonin from scutellaria baicalensis extraction waste residues, which comprises the following steps: firstly, extracting the waste residues by adopting an ethanol solution, heating an extracting solution, adjusting the pH value, crystallizing to obtain a crude baicalin product, and refluxing and purifying by using ethanol to obtain the baicalin; the method comprises the following steps: respectively adjusting the pH value of mother liquor, concentrating, combining, extracting by using a water-saturated ethyl acetate-n-butyl alcohol mixed solvent, concentrating an extract phase, diluting by using ethanol, heating, adjusting the pH value, and crystallizing to obtain baicalein; and further adjusting the pH value of the residual filtrate, dealcoholizing, concentrating, cooling and crystallizing, and refining the obtained solid to obtain wogonin. According to the method disclosed by the invention, extraction, fractional precipitation and solvent extraction are combined for use, and the process conditions of each stage are optimized, so that high-purity baicalin, baicalein and wogonin are prepared from the scutellaria baicalensis extraction waste residues, and the utilization value of the waste residues is improved.

The invention provides a pharmaceutical composition for treating cholestatic liver injury, and the pharmaceutical composition contains an effective amount of Ferulic acid and Wogonin. The application creatively focuses on two key links in the cholestatic liver injury process, namely (1) bile acid excretion and (2) intestinal mucous barrier protection, a synergistic component of Ferulic acid and wogonin is provided, and the optimal ratio of Ferulic acid and wogonin is verified through an experimental system. Experiments prove that the cholestatic liver injury can be effectively improved by combined application of the Ferric acid and the Wogonin which are derived from traditional Chinese medicines; in addition, the effect of the medicine composition is obviously superior to the effect of independently using the Ferric acid or the Wogonin. Wide application prospects are realized.

The invention provides a method for detecting the contents of multiple index components of a coughing oral liquid, which comprises the following steps of: determining the contents of nine key pharmacodynamic index components, namely ephedrine hydrochloride, pseudoephedrine hydrochloride, apiose liquiritin, isoliquiritin, baicalin, huperzin A glycoside, wogonin, baicalein and wogonin; the detection method has the advantages of simplicity and convenience in operation, high stability, high pertinence and high practicability, can be used as a detection and evaluation method for production and internal quality components of the coughing oral liquid, and has a good market application prospect.

The invention belongs to the technical field of medicines, and discloses wogonin shown in a formula (I) and application of pharmacodynamically acceptable salts thereof in preparation of medicines for preventing, relieving and / or treating autoimmune demyelination diseases. On a mouse experimental autoimmune encephalomyelitis model, wogonin shows a good treatment effect, and can effectively prevent and treat pathological changes and disease progression, relieve disease severity and inhibit LPS-stimulated microglial BV2 cells from releasing an inflammatory mediator, thereby providing a new drug and a new structure for clinical treatment.

The present application relates to the technical field of plant extraction, and particularly relates to a process for simultaneously preparing baicalein and wogonin, which comprises the following steps: (1) crushing the roots of Scutellaria baicalensis Georgi and adding boiling water to extract, and combining the extract; (2) taking the extract, concentrating and adjusting the pH for standby; (3) taking the concentrated solution, heating, cooling, adjusting the pH, and collecting the lysate; (4) taking the lysate, filtering twice, and collecting the second filtrate; (5) taking the second filtrate, adjusting the pH, filtering, collecting the first filtrate and the first filter cake; (6) adding purified water to the first filter cake to make pulp, filtering, drying the collected second filter cake, and obtaining the baicalein product; (7) concentrating, crystallizing and filtering the first filtrate, drying the obtained third filtrate, obtaining the scutellarein product, adding purified water to the third filter cake to make pulp, filtering, drying the obtained fourth filter cake, and obtaining the wogonin product. The process can simultaneously obtain high-content baicalein, wogonin and scutellarein products.

The invention relates to the technical field of fermentation engineering and medicines, and discloses a method for producing wogonin and hispidulin through synchronous transformation, which comprises the following steps: 1) preparing a fermentation culture medium; and 2) carrying out solid biotransformation. The wild schizophyllum commune is utilized to perform solid transformation on the traditional Chinese medicine scutellaria baicalensis, synchronous transformation production of wogonin and hispidulin is realized, the drug effects of the solid transformation product and schizophyllum commune polysaccharide are synergistically improved, and the solid transformation product can be directly used without extraction. The method is simple to operate and high in conversion efficiency, particularly the hispidulin is converted and synthesized for the first time, and the method has important application prospects and economic benefits in the field of medicines.