A special air-permeable biological bacteriostasis pad for cotton seedling cultivation is prepared from 200-300 parts of rice straws, 20-30 parts of peanut shells, 15-25 parts of wheat straws, 20-30 parts of rice hulls, 2-4 parts of bone meal, 20-30 parts of leaf mold, 30-40 parts of plant ash, 2-4 parts of slaked lime, 60-70 parts of a nutrient solution, 3-5 parts of polysaccharide algin, 2-4 parts of an insectifuge extracted from various chrysanthemum flowers, named as Kujusu, 2-4 parts of Heliobacterium, 3-5 parts of sweet wormwood herb, 2-4 parts of Radix Euphorbiae Ebractealatae, 3-5 parts of Artemisia capillaries, 2-4 parts of Common Threewingnut Root, 2-4 parts of Radix Stemonae, 3-5 parts of rheum officinale, 2-4 parts of berberine, 1-3 parts of scutelloside and 2-4 parts of Falsehellebore Root and Rhizome. A making method of the special air-permeable biological bacteriostasis pad is simple; the pad contains nutrients needed by traditional seedbed matrixes, and seedbeds are made through adopting loose straws as a matrix, so the air permeability of the seedbeds is guaranteed, and superior environment needed by growth of cotton is provided, thereby cotton seedlings are nutritional and have good environment; and bacteriostasis Chinese patent medicinal materials are added to realize the efficacy of bacteriostasis and insect disinfestations, so healthiness and vigorousness are guaranteed in the cotton cultivation process.