The invention relates to a preparation method of a dentritic tetrameric cationic quaternary ammonium surface activity monomer, which includes steps of fully mixing methyl acrylate and monomer 3, 3-diamido-N-methyldipropylamine and then performing addition reaction; adding 6- bromine-1-hexene to perform quaternization reaction; depressurizing and removing solvent; then adding absolute ether as precipitator to wash for three times; adding N, N-dimethyl-1, 3-propane diamine to perform amidation; depressurizing and removing excessive N, N-dimethyl-1, 3-propane diamine to perform amidation; addinglong-chained monomer 1-dodecyl bromide to perform quaternary amination reaction; after finishing the reaction, repeatedly washing for over three times to obtain the pure dentritic tetrameric cationicquaternary ammonium surface activity monomer. The preparation method is easy to obtain raw materials, simple in synthesis step and product purification, high in yield, safe in use, convenient to store, low in temperature required by the process, simple and easy-controlled operation, low in energy consumption, wide in application prospect; the preparation method is applicable to the industrial production.