129 results about "Hydroxytyrosol" patented technology

The invention discloses a hydroxytyrosol-ergothioneine eutectic crystal as well as a preparation method and application thereof, and belongs to the technical fields of medicines, foods, daily chemicals and the like. The invention provides a hydroxytyrosol-ergothioneine eutectic crystal. The X-ray powder diffraction pattern of the hydroxytyrosol-ergothioneine eutectic crystal has characteristic diffraction peaks at least corresponding to the positions where the 2 theta value is 4.21 degrees + / -0.2 degrees, 8.36 degrees + / -0.2 degrees, 12.29 degrees + / -0.2 degrees, 15.29 degrees + / -0.2 degrees, 19.40 degrees + / -0.2 degrees, 20.98 degrees + / -0.2 degrees, 23.12 degrees + / -0.2 degrees and 28.73 degrees + / -0.2 degrees. According to the hydroxytyrosol-ergothioneine eutectic, the melting point of hydroxytyrosol is increased, the hygroscopicity of hydroxytyrosol is remarkably reduced, and meanwhile the stability of hydroxytyrosol and ergothioneine is enhanced. In addition, after the ergothioneine hydroxytyrosol forms the eutectic crystal, the ergothioneine hydroxytyrosol can also play better anti-oxidation and anti-aging effects.

The invention relates to an anti-oxidation composition based on a reverse micelle synergistic technology and a preparation method and application thereof, the anti-oxidation composition comprises a reverse micelle structure and an aqueous solution containing an anti-oxidation active matter, and the reverse micelle structure wraps the aqueous solution containing the anti-oxidation active matter. The reverse micelle structure is used for wrapping the water-soluble antioxidant active matter, oxidation or degradation discoloration of the water-soluble antioxidant active matter is prevented, after the antioxidant active matter such as hydroxytyrosol, ergothioneine and dipotassium glycyrrhizinate is wrapped by the reverse micelle, the stability and the effect are remarkably improved, and after the reverse micelle wrapping, the effect of the antioxidant active matter is improved by 17% or above compared with an aqueous solution before wrapping.

The invention discloses an anti-aging face cream suitable for sensitive skin and a preparation method of the anti-aging face cream. The anti-aging face cream comprises 1-10 wt% of a supramolecular eutectic compound, 10-30 wt% of an auxiliary material and the balance of deionized water, the supramolecular eutectic compound comprises an anti-aging component, a supramolecular eutectic agent and Neosol-Aqulio, wherein the supramolecular eutectic agent is used for forming a supramolecular eutectic with the anti-aging component, and the Neosol-Aqulio is dispersed in the supramolecular eutectic; wherein the anti-aging component comprises oleuropein and hydroxytyrosol, and the supramolecular eutectic agent is nicotinamide, or a mixture of capryloyl glycine and betaine. Neosol-Aqulio, a supramolecular eutectic agent and an anti-aging component are utilized to form a supramolecular eutectic, so that active component delivery is facilitated, efficient transdermal absorption is realized, permeability, stability and mildness of the anti-aging active component are improved, the problem of sensitive skin cuticle permeation is solved, irritation of a traditional penetration enhancer is avoided, and the penetration enhancer is suitable for clinical application. The double effects of efficient permeation and mild anti-aging are realized.

The invention provides a hydroxytyrosol supramolecular compound and a preparation method and application thereof, and belongs to the technical field of cosmetics and biological medicine, the supramolecular compound comprises the following chemical components by mass: 0.01-10% of hydroxytyrosol, 2-45% of an amino acid compound, 2-20% of alpha hydroxy acid, 2-30% of urea, and 15-92% of water. The supramolecular compound can promote transdermal delivery of hydroxytyrosol and maintain the biological activity of hydroxytyrosol, shows high biocompatibility, and can be applied to product development in the antibacterial field, the regeneration field and the anti-aging field.

The invention provides a hydroxytyrosol-loaded soluble microneedle as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The microneedle comprises a substrate layer and a needle body arranged on the surface of the substrate layer, the needle body comprises 0.01%-20% of hydroxytyrosol and 1%-10% of hyaluronic acid, the molecular weight of the hyaluronic acid is 100-1000 kDa, and the% is mass percentage content w / w or mass volume percentage content w / v. The soluble microneedle is mild and controllable in preparation process, does not need high temperature, is simple in preparation and use method, and is green, safe and pollution-free; the matrix material is simple, no organic solvent is used, only water is used as a solvent and an adhesive, and no extra cross-linking agent needs to be added; the hydrogel is good in needle forming property, good in biocompatibility, high in safety, high in mechanical strength, excellent in transdermal drug delivery effect and capable of remarkably improving skin photoaging.

The invention belongs to the technical field of functional food processing, and particularly relates to a method for synergistically improving functional characteristics by utilizing casein peptide and hydroxytyrosol self-assembly. According to the research, casein peptide with an excellent antioxidant function is used as a raw material, and hydroxytyrosol is added to be self-assembled with the casein peptide, so that the antioxidant activity is synergistically improved in an in-vitro digestion stage. Compared with a traditional protein and polyphenol combination technology, the casein peptide and polyphenol self-assembly method is provided, the antioxidant activity after digestion is improved, no toxic chemical reagent is introduced or added, and the casein peptide and polyphenol self-assembly method is safer, more efficient, more environmentally friendly and more sustainable. The method can be widely applied to functional food or health care products.

The invention discloses modified hydroxytyrosol as well as a preparation method and application thereof. The provided preparation method of the modified hydroxytyrosol comprises the following steps: in the presence of an acidic solvent, mixing hydroxytyrosol, protease and collagen for reaction, mixing a reaction product with an extraction agent, performing centrifugal layering, and collecting upper-layer components, thereby obtaining the modified hydroxytyrosol, the protease comprises one or two of neutral protease and trypsin. Under the enzymolysis action of protease, the collagen can be decomposed into amino acid monomers such as glycine, proline and hydroxyproline, and the amino acid monomers can be subjected to graft modification with hydroxytyrosol; meanwhile, collagen fragments which are not subjected to enzymolysis also exist, and are subjected to graft modification with hydroxytyrosol in the form of collagen characteristic fragments. The oxidation resistance and the stability of the obtained modified hydroxytyrosol are obviously improved compared with those of unmodified hydroxytyrosol.

The invention discloses modified hydroxytyrosol as well as a preparation method and application thereof. The provided preparation method of the modified hydroxytyrosol comprises the following steps: in the presence of an acidic buffer solution, mixing hydroxytyrosol and vitamin C ethyl ether, and carrying out a dehydration condensation reaction to prepare the modified hydroxytyrosol. According to the provided preparation method of the modified hydroxytyrosol, the vitamin C ethyl ether is adopted to modify the hydroxytyrosol, the preparation method is simple and easy to operate, and the prepared modified hydroxytyrosol has high oxidation resistance and inflammation resistance.

The invention discloses modified hydroxytyrosol as well as a preparation method, cosmetics and application thereof. The provided preparation method of the modified hydroxytyrosol comprises the following steps: in the presence of an acidic solvent, hydroxytyrosol and bromelain are mixed for an enzymatic hydrolysis reaction, and the modified hydroxytyrosol is prepared. According to the provided preparation method of the modified hydroxytyrosol, the hydroxytyrosol is subjected to catalytic modification through bromelain, bromelain serves as typical sulfydryl protease and is subjected to an enzymolysis reaction with the hydroxytyrosol, the hydroxytyrosol enzymolysis derivative is generated, and the generated hydroxytyrosol enzymolysis derivative contains various substances with aromatic smells, so that the hydroxytyrosol can be used for preparing the modified hydroxytyrosol. The obvious pungent smell of the hydroxytyrosol raw material is effectively improved.

The invention relates to the technical field of active ingredient extraction, in particular to an ergothioneine-hydroxytyrosol mixture and a preparation method and application thereof, and optimizes a culture medium formula for producing ergothioneine through submerged liquid fermentation of pleurotus citrinopileatus mycelium. In the later stage of the process of producing ergothioneine by mycelium fermentation, a culture medium formula is designed according to nutritional ingredients required by hydroxytyrosol biosynthesis to supplement a sterile culture medium, and meanwhile, secondary metabolites and residual nutritional ingredients generated by fermentation of pleurotus citrinopileatus mycelium are fully utilized; the ergothioneine-hydroxytyrosol mixture is obtained through biosynthesis fermentation of hydroxytyrosol, the production period is short, the efficiency is high, the energy consumption is low, the ergothioneine-hydroxytyrosol mixture is green and safe, nutritional ingredients of a culture solution can be fully utilized, the overall production cost is reduced, the comprehensive production efficiency and productivity are improved, no waste liquid is discharged, and the subsequent application requirements are better met.

The invention discloses modified hydroxytyrosol, a cosmetic, a preparation method and application. The provided preparation method of the modified hydroxytyrosol comprises the following steps: in the presence of an acidic solvent, mixing hydroxytyrosol, lipase and fatty acid ester for reaction, centrifugally layering a reaction product, and collecting components of a middle layer to prepare the modified hydroxytyrosol. According to the provided preparation method of the modified hydroxytyrosol, lipase and fatty acid ester are subjected to an enzyme digestion reaction, an active hydroxyl group in a product obtained through the enzyme digestion reaction and hydroxytyrosol are subjected to a dehydration reaction, so that an ester group molecular chain is grafted into hydroxytyrosol, and a modified hydroxytyrosol product with an ester group is prepared. And the obtained modified hydroxytyrosol has relatively high fat solubility and oxidation resistance.

The invention discloses saccharomyces cerevisiae with high yield of salidroside as well as a construction method and application of the saccharomyces cerevisiae. According to the recombinant saccharomyces cerevisiae, ribulose-5-phosphate isomerase RKI1 and transketolase TKL1 are expressed in host bacteria, so that precursor supply is increased; reverse methyltransferase ARO2 and phenylalanine decarboxylase ARO10 are expressed at the same time, and a tyrosine branch pathway is adjusted; then, UDP-glycosyl transferase of different sources is expressed, and iterative site-specific mutagenesis is carried out on the UDP-glycosyl transferase to enhance conversion of hydroxytyrosol to salidroside; the salidroside production performance of the recombinant strain is verified on the basis of a precursor supply pathway, a tyrosine regulation pathway and key enzyme screening, and the salidroside production capacity of the saccharomyces cerevisiae is further improved.

The invention discloses modified hydroxytyrosol, flavored hydroxytyrosol, a preparation method of the modified hydroxytyrosol, a preparation method of the flavored hydroxytyrosol, functional food and cosmetics. Activated carbon loaded p-toluenesulfonic acid is used as a catalyst to catalyze papain to modify hydroxytyrosol, so that the effect activity of the modified hydroxytyrosol raw material is reserved, and meanwhile, the bad flavor generated by impurities in industrial production of the hydroxytyrosol raw material is remarkably reduced; furthermore, the smell and the flavor of the hydroxytyrosol raw material are remarkably improved, the application effect of the hydroxytyrosol raw material in related fields is improved, and the use sensory experience of the product is improved. The flavor of hydroxytyrosol is improved in an enzyme preparation catalysis mode, and the process method is green and safe.

The application discloses a chemical synthesis method of hydroxytyrosol glucoside. The method uses hydroxytyrosol as a starting material, and acetyl protection, glycosylation, hydrolysis and other steps are performed to prepare the hydroxytyrosol glucoside. The synthesis method of the hydroxytyrosol glucoside has the characteristics of low cost, simple process and industrialization.

Triphenylphosphonium hydroxytyrosol TPP-HT, its synthesis method and application in the preparation of drugs for improving aortic endothelial cell function. TPP-HT can significantly increase the reduction of human aortic endothelial cell activity caused by saturated fatty acids, inhibit the inflammatory response and oxidative stress caused by saturated fatty acids, and at the same time increase the synthesis of ATP in vascular endothelial cells and the expression of mitochondrial complex II. By anti-inflammatory and protecting mitochondrial function, it can prevent the occurrence and development of atherosclerosis. Therefore, TPP-HT has good application prospects in preventing cardiovascular diseases such as atherosclerosis caused by high-fat-induced endothelial damage, opening up a new medical approach for preventing a series of problems of cardiovascular diseases such as atherosclerosis caused by endothelial damage due to unbalanced dietary structure, and providing a new basis for the development of new drugs.

The present invention discloses a method for synthesizing hydroxytyrosol by multi-enzyme cascade, comprising: 1) preparing a hydroxytyrosol product by using 3,4-dihydroxybenzaldehyde, L-threonine, sodium formate, NAD + As raw material, L-threonine transaldolase, alcohol dehydrogenase, and formate dehydrogenase are used to catalyze the synthesis of L-threo-3-(3,4-dihydroxyphenyl)serine; 2) L-threo-3-(3,4-dihydroxyphenyl)serine is used as substrate, glucose, NAD + Hydroxytyrosol is synthesized through a cascade catalysis of phenylserine dehydratase, α-ketoacid decarboxylase, aldehyde reductase, and glucose dehydrogenase. The synthesis method provided by the present invention achieves a hydroxytyrosol yield of >99% and a space-time yield of 0.88 g / L / h, currently the highest, showing great potential for industrial application and suitable for further promotion and application.

The application discloses a green preparation method of a medium-chain acylated hydroxytyrosol lipoid-triphenylphosphine derivative and application of the derivative in mitochondrial-targeted antioxidation, and relates to the following steps: taking hydroxytyrosol and saturated halogenated fatty acids with medium-chain length C8-C12 as raw materials, and synthesizing a medium-chain hydroxytyrosol lipoid compound through a low-field ultrasonic coupling immobilized lipase synergistic directional alcohol hydroxyl acylation reaction; and further synthesizing the lipoid compound through high-speed stirring reaction with a triphenylphosphine compound to obtain a medium-chain acylated hydroxytyrosol lipoid-triphenylphosphine derivative. The derivative has the antioxidation effect of mitochondrial-targeted enrichment and active oxygen radical scavenging. The hydroxytyrosol derivative prepared by the application has the characteristics of green efficiency, directional bonding and simple operation. The acylation reaction of hydroxytyrosol does not need to involve flammable and explosive, easily oxidized and corrosive concentrated sulfuric acid and concentrated nitric acid, and the medium-chain acylated hydroxytyrosol lipoid-triphenylphosphine derivative has mitochondrial targeting, can be used as a potential mitochondrial nutritional supplement product, and has a wide application prospect in the health care functions of anti-aging, cardiovascular protection, diabetes prevention and the like.

The application provides a kind of dynamic monitoring method and system of olive leaf polyphenol components based on principal component analysis, it is related to computer technology field, comprising: obtaining original data;According to the original data, the spectrum interval is separated, and the purified polyphenol characteristic spectrum sequence is obtained;According to the purified polyphenol characteristic spectrum sequence, spectral deconvolution processing is carried out, and the polyphenol subcomponent concentration evolution matrix is obtained;According to the polyphenol subcomponent concentration evolution matrix, the principal component is reconstructed, and the reconstruction result is obtained;According to the reconstruction result, track fitting is carried out, by fitting the principal component score track into the kinetic network model containing hydroxytyrosol glycoside hydrolysis and oxidative condensation step, the fitting result is obtained;According to the fitting result, prediction is carried out, the evolution end point of hydroxytyrosol free state content and its derivative relative proportion is predicted, and the dynamic monitoring result is obtained.The application realizes the accurate monitoring and prediction of the dynamic conversion process of specific polyphenol components such as hydroxytyrosol glycoside and oleuropein in olive leaves.

The invention relates to the technical field of spectral analysis, in particular to a hydroxytyrosol content detection method and system for food quality inspection.The method comprises the steps that spectral data of an olive oil food sample are collected, liquid chromatographic data of the food sample are obtained, a peak point, a chromatographic peak sequence and a chromatographic peak half-and-half sequence are determined, a chromatographic peak shape interference coefficient is obtained, and a hydroxytyrosol content detection result is obtained. Acquiring a peak shape symmetric inclination coefficient based on the chromatographic peak shape interference coefficient and the difference before and after peak points in the chromatographic peak half-and-half sequence, determining a self-adaptive asymmetric penalty coefficient, acquiring chromatographic data of the corrected olive oil food sample, and judging whether the content of hydroxytyrosol in the olive oil food sample reaches the standard or not. The method aims at solving the problems that in a traditional BEADS algorithm, an asymmetric penalty factor cannot be dynamically adjusted according to the liquid chromatogram data of the olive oil food sample, so that the liquid chromatogram data of the olive oil food sample is excessively corrected and is not sufficiently corrected.

The invention discloses a recombinant microorganism for producing hydroxytyrosol as well as a construction method and application of the recombinant microorganism. According to the recombinant microorganism, 3-deoxy-D-arabinoheptulose-7-phosphate synthetase aroGfbr, chorismate mutase / pre-benzoic acid dehydrogenase tyrAfbr, 4-hydroxyphenylacetic acid 3-hydroxylase HpaBC, phenylpyruvate decarboxylase ARO10 and alcohol dehydrogenase ADH6 are subjected to overexpression, and the recombinant microorganism can be used for preparing the recombinant microorganism. The genome of the gene naturally comprises a citrate synthase coding gene gltA and a tyrosine aminotransferase coding gene tyrB; the original promoter of the gltA and / or the original promoter of the tyrB are / is replaced by a time sequence promoter PrrnC-37. When the recombinant microorganism is used for producing hydroxytyrosol, the production efficiency can be improved, and the production cost of hydroxytyrosol can be reduced.

The application discloses a kind of flame-retardant bio-based copolymer resin based on PT-fa, to obtain mixed resin with bio-based benzoxazine monomer PT-fa and bisphenol A-aniline type benzoxazine BA-a as raw material, then, mixed resin is heat cured to obtain flame-retardant bio-based copolymer resin Poly (PT-fa / BA-a) based on PT-fa, the obtained copolymer resin has high thermal stability and high flame retardant performance;The PT-fa is prepared by solvent-free synthesis method with hydroxyl tyrosol, furfurylamine and polyformaldehyde as raw material, the obtained monomer has high purity.The glass transition temperature T g of the Poly (PT-fa / BA-a) is 180-300 DEG C;The temperature T di of 5% thermal weight loss under nitrogen atmosphere is 328-340 DEG C, the carbon residue rate Y c at 800 DEG C is 38-60%;Heat release capacity HRC is 80-175 J / g·K, and the total heat release THR is 10-21 kJ / g.The preparation method comprises the following steps: 1, the preparation of bio-based benzoxazine monomer PT-fa;2, the preparation of flame-retardant bio-based copolymer resin based on PT-fa.

The present application relates to a kind of synthetic hydroxytyrosol multi-enzyme catalytic method.The synthetic hydroxytyrosol multi-enzyme catalytic method described in the present application includes first module reaction, second module reaction, third module reaction in turn;The first module reaction is that glycerol is synthesized pyruvic acid by catalysis, wherein the concentration of the glycerol is 1-5mM;The second module reaction is that pyruvic acid is condensed with catechol and ammonium chloride to be L-levodopa by catalysis;Wherein, the concentration of the catechol is 1-5mM, and the concentration of the catechol is greater than the concentration of the glycerol in the first module reaction;The third module reaction is that L-levodopa is synthesized hydroxytyrosol by catalysis.The synthetic hydroxytyrosol multi-enzyme catalytic method described in the present application, reaction 4 hours hydroxytyrosol molar conversion rate can reach 71.5%, compared with one-pot method has obvious advantage, in the same reaction time molar conversion rate is compared with one-pot method and increased nearly 6 times.

The invention relates to an efficient multifunctional cleaning agent for a medical endoscope and a preparation method of the efficient multifunctional cleaning agent, and belongs to the technical field of medical cleaning. The cleaning agent is prepared from isopropyl alcohol, glycerol, propylene glycol, TritonX-100, fatty alcohol-polyoxyethylene ether, mannitol, 4-FPBA, hydroxytyrosol, sodium formate, benzisothiazolinone, a multienzyme compound and deionized water according to a specific ratio. The preparation method comprises the steps of step-by-step dissolution, temperature-controlled mixing and low-temperature enzyme adding. The cleaning agent can effectively remove stubborn stains such as protein and fat on the surface of an endoscope (the removal rate is larger than or equal to 90%), the bacteriostasis rate on escherichia coli and staphylococcus aureus is larger than or equal to 99%, and corrosion to stainless steel, rubber and plastic materials is avoided. Experiments show that the enzyme activity retention rate of the multienzyme compound still reaches 80% or above after the multienzyme compound is stored for 2 years, and the technical problems that an existing cleaning agent is not thorough in decontamination, poor in antibacterial activity and capable of corroding equipment are solved.

A composition containing nicotinamide mononucleotide (NMN) or an analog thereof, and certain antioxidants, more specifically resveratrol and at least one of ergothioneine and hydroxytyrosol, can be used in an anti-aging and / or anti-inflammatory method. The composition can contain NMN or nicotinamide riboside (NR) in an amount of from about 101 mg to about 1500 mg; the resveratrol in an amount of from about 50 mg to about 500 mg; ergothioneine or salt thereof, when present, in an amount of from about 3 mg to about 25 mg; and the hydroxytyrosol, when present, in an amount of from about 20 mg to about 100 mg.

The present invention relates to the field of biogenic nitrogen fertilizer synergists, and in particular to an application of an olive pomace polyphenol extract-hydroxytyrosol as a biogenic nitrogen fertilizer synergist. The biogenic nitrogen fertilizer synergist component prepared by the present invention is an olive pomace polyphenol extract-hydroxytyrosol. After the olive pomace is deoiled, it is extracted under acid hydrolysis conditions, and an alkaline solution of sodium hydroxide is used to adjust the pH value to neutral to obtain the corresponding hydroxytyrosol. The abundance of soil nitrifying microorganisms (ammonia oxidizing bacteria and ammonia oxidizing archaea) and the conversion of mineral nitrogen in the soil can be effectively suppressed, the effectiveness of nitrogen in the soil can be improved, nitrogen can be more effectively absorbed and utilized by crops, the olive pomace is effectively recycled, and the problems of huge environmental pollution and waste of resources caused by the olive pomace can be effectively avoided.

The invention discloses a method for sterilizing a vegetable protein beverage, which comprises the following steps of: adding a composite antibacterial microsphere preparation into a vegetable protein beverage to be treated, uniformly mixing, and performing high-density CO2 treatment and magnetic induction electric field sterilization to obtain a vegetable protein beverage finished product, the composite antibacterial microsphere preparation is prepared by taking fucoidan and a gallic acid acylated quercetin-phospholipid conjugate as a substrate and loading lysozyme and hydroxytyrosol. High-density CO2 and a magnetic induction electric field are adopted for synergistic sterilization, so that the structure of a microbial film can be changed, metabolism is disturbed, a good matrix is provided for electric field sterilization, and the sterilization efficiency is further improved through electric breakdown, magnetic field refining of water molecular groups and heat effect; the composite microspheres are sensitive to an electric field, so that lysozyme and hydroxytyrosol can be triggered to be accurately and quickly released under the action of a magnetic induction electric field, stubborn spores can be efficiently killed, and the killing effect is improved by destroying cell wall structures and disulfide bonds of the spores; meanwhile, protein aggregation and flocculation in the beverage can be effectively prevented, and the stability of a beverage system is guaranteed.

The invention discloses temperature-induced Escherichia coli for high yield of tyrosol and hydroxytyrosol as well as a construction method and application of the temperature-induced Escherichia coli, and belongs to the technical field of microbial synthesis. On the basis of escherichia coli with high tyrosine yield, a temperature induction system is utilized for the first time, AroGfbr, ARO10, ADHs and TyrAfbr genes are over-expressed, and de novo synthesis of tyrosol is achieved; and screening ARO10 and ADHs genes to obtain the strain with high yield of tyrosol. On the basis, an HpaB / C gene is over-expressed, meanwhile, pykA, tyrB and mhpB genes are knocked out on a genome, glf and glk genes are knocked into a ptsG site, the yield of hydroxytyrosol is remarkably increased, the shake flask yield reaches 1.28 g / L, and the yield of a 5L reactor reaches 4.97 g / L. The method lays a foundation for low-cost and high-efficiency industrial production of tyrosol and hydroxytyrosol, and has a wide application prospect.

The invention is applicable to the technical field of hydroxytyrosol preparation, and provides a method for preparing hydroxytyrosol by combining compound enzyme with molecular imprinting adsorption, which comprises the following steps: crushing fresh olive fruits, and collecting olive fruit pulp; putting the olive pulp into an enzymolysis tank, adding cellulose-pectin compound enzyme and citric acid, and performing enzymolysis; transferring the olive pulp subjected to enzymolysis into a microwave extraction tank, adding purified water, carrying out intermittent irradiation extraction, centrifuging after the extraction is completed, collecting supernate, and carrying out vacuum concentration; and adsorbing the concentrated supernate by using a chromatographic column filled with a hydroxytyrosol imprinted polymer, eluting with water and an ethanol solution in sequence, collecting ethanol eluent, and concentrating to obtain extract, namely the high-purity hydroxytyrosol. The problems that an existing fermentation method is low in yield and high in cost are solved. Generation of byproducts is reduced, and the separation and purification process is simplified; green and sustainable hydroxytyrosol industrial production is realized; and the purification period is shortened to be within 5 hours.

Compositions for treatment or prevention of synovitis contain a therapeutically effective amount of at least one of oleuropein, rutin or curcumin and preferably contain at least oleuropin. Methods for treatment or prevention of synovitis include administering such compositions. The compositions can additionally include one or more of quercetin, hydroxytyrosol or vitamin D.

The invention provides an extraction method for increasing the oleuropein conversion rate and the hydroxytyrosol content in olive pomace, and belongs to the technical field of natural product extraction. Based on the analysis of the characteristics of the olive pomace material, firstly, the compact structure of the olive pomace is destroyed through steam explosion pretreatment, and pores are loosened; then carrying out compound enzyme enzymolysis, cooperatively hydrolyzing wrapped binding protein by adopting acid protease and hemicellulase in a combined manner, destroying a polysaccharide wrapping barrier in the pomace, and promoting rapid release of oleuropein; finally, high static pressure extraction is adopted, ultrahigh pressure is used for accelerating solvent permeation, the conversion rate of oleuropein is increased, and the content of hydroxytyrosol is comprehensively increased. The method is green and efficient, the oleuropein conversion rate can be increased to the maximum extent, and the content of hydroxytyrosol is increased; the degradation loss of hydroxytyrosol in the extraction process is reduced, and the economic benefits of the olive pomace are comprehensively improved.