84 results about "Hydroxytyrosol" patented technology

The invention discloses a hydroxytyrosol-ergothioneine eutectic crystal as well as a preparation method and application thereof, and belongs to the technical fields of medicines, foods, daily chemicals and the like. The invention provides a hydroxytyrosol-ergothioneine eutectic crystal. The X-ray powder diffraction pattern of the hydroxytyrosol-ergothioneine eutectic crystal has characteristic diffraction peaks at least corresponding to the positions where the 2 theta value is 4.21 degrees + / -0.2 degrees, 8.36 degrees + / -0.2 degrees, 12.29 degrees + / -0.2 degrees, 15.29 degrees + / -0.2 degrees, 19.40 degrees + / -0.2 degrees, 20.98 degrees + / -0.2 degrees, 23.12 degrees + / -0.2 degrees and 28.73 degrees + / -0.2 degrees. According to the hydroxytyrosol-ergothioneine eutectic, the melting point of hydroxytyrosol is increased, the hygroscopicity of hydroxytyrosol is remarkably reduced, and meanwhile the stability of hydroxytyrosol and ergothioneine is enhanced. In addition, after the ergothioneine hydroxytyrosol forms the eutectic crystal, the ergothioneine hydroxytyrosol can also play better anti-oxidation and anti-aging effects.

The invention discloses an anti-aging face cream suitable for sensitive skin and a preparation method of the anti-aging face cream. The anti-aging face cream comprises 1-10 wt% of a supramolecular eutectic compound, 10-30 wt% of an auxiliary material and the balance of deionized water, the supramolecular eutectic compound comprises an anti-aging component, a supramolecular eutectic agent and Neosol-Aqulio, wherein the supramolecular eutectic agent is used for forming a supramolecular eutectic with the anti-aging component, and the Neosol-Aqulio is dispersed in the supramolecular eutectic; wherein the anti-aging component comprises oleuropein and hydroxytyrosol, and the supramolecular eutectic agent is nicotinamide, or a mixture of capryloyl glycine and betaine. Neosol-Aqulio, a supramolecular eutectic agent and an anti-aging component are utilized to form a supramolecular eutectic, so that active component delivery is facilitated, efficient transdermal absorption is realized, permeability, stability and mildness of the anti-aging active component are improved, the problem of sensitive skin cuticle permeation is solved, irritation of a traditional penetration enhancer is avoided, and the penetration enhancer is suitable for clinical application. The double effects of efficient permeation and mild anti-aging are realized.

The invention provides a hydroxytyrosol supramolecular compound and a preparation method and application thereof, and belongs to the technical field of cosmetics and biological medicine, the supramolecular compound comprises the following chemical components by mass: 0.01-10% of hydroxytyrosol, 2-45% of an amino acid compound, 2-20% of alpha hydroxy acid, 2-30% of urea, and 15-92% of water. The supramolecular compound can promote transdermal delivery of hydroxytyrosol and maintain the biological activity of hydroxytyrosol, shows high biocompatibility, and can be applied to product development in the antibacterial field, the regeneration field and the anti-aging field.

The invention discloses modified hydroxytyrosol as well as a preparation method and application thereof. The provided preparation method of the modified hydroxytyrosol comprises the following steps: in the presence of an acidic solvent, mixing hydroxytyrosol, protease and collagen for reaction, mixing a reaction product with an extraction agent, performing centrifugal layering, and collecting upper-layer components, thereby obtaining the modified hydroxytyrosol, the protease comprises one or two of neutral protease and trypsin. Under the enzymolysis action of protease, the collagen can be decomposed into amino acid monomers such as glycine, proline and hydroxyproline, and the amino acid monomers can be subjected to graft modification with hydroxytyrosol; meanwhile, collagen fragments which are not subjected to enzymolysis also exist, and are subjected to graft modification with hydroxytyrosol in the form of collagen characteristic fragments. The oxidation resistance and the stability of the obtained modified hydroxytyrosol are obviously improved compared with those of unmodified hydroxytyrosol.

The invention discloses modified hydroxytyrosol as well as a preparation method and application thereof. The provided preparation method of the modified hydroxytyrosol comprises the following steps: in the presence of an acidic buffer solution, mixing hydroxytyrosol and vitamin C ethyl ether, and carrying out a dehydration condensation reaction to prepare the modified hydroxytyrosol. According to the provided preparation method of the modified hydroxytyrosol, the vitamin C ethyl ether is adopted to modify the hydroxytyrosol, the preparation method is simple and easy to operate, and the prepared modified hydroxytyrosol has high oxidation resistance and inflammation resistance.

The invention discloses modified hydroxytyrosol as well as a preparation method, cosmetics and application thereof. The provided preparation method of the modified hydroxytyrosol comprises the following steps: in the presence of an acidic solvent, hydroxytyrosol and bromelain are mixed for an enzymatic hydrolysis reaction, and the modified hydroxytyrosol is prepared. According to the provided preparation method of the modified hydroxytyrosol, the hydroxytyrosol is subjected to catalytic modification through bromelain, bromelain serves as typical sulfydryl protease and is subjected to an enzymolysis reaction with the hydroxytyrosol, the hydroxytyrosol enzymolysis derivative is generated, and the generated hydroxytyrosol enzymolysis derivative contains various substances with aromatic smells, so that the hydroxytyrosol can be used for preparing the modified hydroxytyrosol. The obvious pungent smell of the hydroxytyrosol raw material is effectively improved.

The invention discloses modified hydroxytyrosol, a cosmetic, a preparation method and application. The provided preparation method of the modified hydroxytyrosol comprises the following steps: in the presence of an acidic solvent, mixing hydroxytyrosol, lipase and fatty acid ester for reaction, centrifugally layering a reaction product, and collecting components of a middle layer to prepare the modified hydroxytyrosol. According to the provided preparation method of the modified hydroxytyrosol, lipase and fatty acid ester are subjected to an enzyme digestion reaction, an active hydroxyl group in a product obtained through the enzyme digestion reaction and hydroxytyrosol are subjected to a dehydration reaction, so that an ester group molecular chain is grafted into hydroxytyrosol, and a modified hydroxytyrosol product with an ester group is prepared. And the obtained modified hydroxytyrosol has relatively high fat solubility and oxidation resistance.

The invention discloses saccharomyces cerevisiae with high yield of salidroside as well as a construction method and application of the saccharomyces cerevisiae. According to the recombinant saccharomyces cerevisiae, ribulose-5-phosphate isomerase RKI1 and transketolase TKL1 are expressed in host bacteria, so that precursor supply is increased; reverse methyltransferase ARO2 and phenylalanine decarboxylase ARO10 are expressed at the same time, and a tyrosine branch pathway is adjusted; then, UDP-glycosyl transferase of different sources is expressed, and iterative site-specific mutagenesis is carried out on the UDP-glycosyl transferase to enhance conversion of hydroxytyrosol to salidroside; the salidroside production performance of the recombinant strain is verified on the basis of a precursor supply pathway, a tyrosine regulation pathway and key enzyme screening, and the salidroside production capacity of the saccharomyces cerevisiae is further improved.

The invention discloses modified hydroxytyrosol, flavored hydroxytyrosol, a preparation method of the modified hydroxytyrosol, a preparation method of the flavored hydroxytyrosol, functional food and cosmetics. Activated carbon loaded p-toluenesulfonic acid is used as a catalyst to catalyze papain to modify hydroxytyrosol, so that the effect activity of the modified hydroxytyrosol raw material is reserved, and meanwhile, the bad flavor generated by impurities in industrial production of the hydroxytyrosol raw material is remarkably reduced; furthermore, the smell and the flavor of the hydroxytyrosol raw material are remarkably improved, the application effect of the hydroxytyrosol raw material in related fields is improved, and the use sensory experience of the product is improved. The flavor of hydroxytyrosol is improved in an enzyme preparation catalysis mode, and the process method is green and safe.

The application discloses a chemical synthesis method of hydroxytyrosol glucoside. The method uses hydroxytyrosol as a starting material, and acetyl protection, glycosylation, hydrolysis and other steps are performed to prepare the hydroxytyrosol glucoside. The synthesis method of the hydroxytyrosol glucoside has the characteristics of low cost, simple process and industrialization.

The application discloses a green preparation method of a medium-chain acylated hydroxytyrosol lipoid-triphenylphosphine derivative and application of the derivative in mitochondrial-targeted antioxidation, and relates to the following steps: taking hydroxytyrosol and saturated halogenated fatty acids with medium-chain length C8-C12 as raw materials, and synthesizing a medium-chain hydroxytyrosol lipoid compound through a low-field ultrasonic coupling immobilized lipase synergistic directional alcohol hydroxyl acylation reaction; and further synthesizing the lipoid compound through high-speed stirring reaction with a triphenylphosphine compound to obtain a medium-chain acylated hydroxytyrosol lipoid-triphenylphosphine derivative. The derivative has the antioxidation effect of mitochondrial-targeted enrichment and active oxygen radical scavenging. The hydroxytyrosol derivative prepared by the application has the characteristics of green efficiency, directional bonding and simple operation. The acylation reaction of hydroxytyrosol does not need to involve flammable and explosive, easily oxidized and corrosive concentrated sulfuric acid and concentrated nitric acid, and the medium-chain acylated hydroxytyrosol lipoid-triphenylphosphine derivative has mitochondrial targeting, can be used as a potential mitochondrial nutritional supplement product, and has a wide application prospect in the health care functions of anti-aging, cardiovascular protection, diabetes prevention and the like.

The application provides a kind of dynamic monitoring method and system of olive leaf polyphenol components based on principal component analysis, it is related to computer technology field, comprising: obtaining original data;According to the original data, the spectrum interval is separated, and the purified polyphenol characteristic spectrum sequence is obtained;According to the purified polyphenol characteristic spectrum sequence, spectral deconvolution processing is carried out, and the polyphenol subcomponent concentration evolution matrix is obtained;According to the polyphenol subcomponent concentration evolution matrix, the principal component is reconstructed, and the reconstruction result is obtained;According to the reconstruction result, track fitting is carried out, by fitting the principal component score track into the kinetic network model containing hydroxytyrosol glycoside hydrolysis and oxidative condensation step, the fitting result is obtained;According to the fitting result, prediction is carried out, the evolution end point of hydroxytyrosol free state content and its derivative relative proportion is predicted, and the dynamic monitoring result is obtained.The application realizes the accurate monitoring and prediction of the dynamic conversion process of specific polyphenol components such as hydroxytyrosol glycoside and oleuropein in olive leaves.

The invention relates to the technical field of spectral analysis, in particular to a hydroxytyrosol content detection method and system for food quality inspection.The method comprises the steps that spectral data of an olive oil food sample are collected, liquid chromatographic data of the food sample are obtained, a peak point, a chromatographic peak sequence and a chromatographic peak half-and-half sequence are determined, a chromatographic peak shape interference coefficient is obtained, and a hydroxytyrosol content detection result is obtained. Acquiring a peak shape symmetric inclination coefficient based on the chromatographic peak shape interference coefficient and the difference before and after peak points in the chromatographic peak half-and-half sequence, determining a self-adaptive asymmetric penalty coefficient, acquiring chromatographic data of the corrected olive oil food sample, and judging whether the content of hydroxytyrosol in the olive oil food sample reaches the standard or not. The method aims at solving the problems that in a traditional BEADS algorithm, an asymmetric penalty factor cannot be dynamically adjusted according to the liquid chromatogram data of the olive oil food sample, so that the liquid chromatogram data of the olive oil food sample is excessively corrected and is not sufficiently corrected.

The invention discloses a recombinant microorganism for producing hydroxytyrosol as well as a construction method and application of the recombinant microorganism. According to the recombinant microorganism, 3-deoxy-D-arabinoheptulose-7-phosphate synthetase aroGfbr, chorismate mutase / pre-benzoic acid dehydrogenase tyrAfbr, 4-hydroxyphenylacetic acid 3-hydroxylase HpaBC, phenylpyruvate decarboxylase ARO10 and alcohol dehydrogenase ADH6 are subjected to overexpression, and the recombinant microorganism can be used for preparing the recombinant microorganism. The genome of the gene naturally comprises a citrate synthase coding gene gltA and a tyrosine aminotransferase coding gene tyrB; the original promoter of the gltA and / or the original promoter of the tyrB are / is replaced by a time sequence promoter PrrnC-37. When the recombinant microorganism is used for producing hydroxytyrosol, the production efficiency can be improved, and the production cost of hydroxytyrosol can be reduced.

The present application relates to a kind of synthetic hydroxytyrosol multi-enzyme catalytic method.The synthetic hydroxytyrosol multi-enzyme catalytic method described in the present application includes first module reaction, second module reaction, third module reaction in turn;The first module reaction is that glycerol is synthesized pyruvic acid by catalysis, wherein the concentration of the glycerol is 1-5mM;The second module reaction is that pyruvic acid is condensed with catechol and ammonium chloride to be L-levodopa by catalysis;Wherein, the concentration of the catechol is 1-5mM, and the concentration of the catechol is greater than the concentration of the glycerol in the first module reaction;The third module reaction is that L-levodopa is synthesized hydroxytyrosol by catalysis.The synthetic hydroxytyrosol multi-enzyme catalytic method described in the present application, reaction 4 hours hydroxytyrosol molar conversion rate can reach 71.5%, compared with one-pot method has obvious advantage, in the same reaction time molar conversion rate is compared with one-pot method and increased nearly 6 times.

The invention relates to an efficient multifunctional cleaning agent for a medical endoscope and a preparation method of the efficient multifunctional cleaning agent, and belongs to the technical field of medical cleaning. The cleaning agent is prepared from isopropyl alcohol, glycerol, propylene glycol, TritonX-100, fatty alcohol-polyoxyethylene ether, mannitol, 4-FPBA, hydroxytyrosol, sodium formate, benzisothiazolinone, a multienzyme compound and deionized water according to a specific ratio. The preparation method comprises the steps of step-by-step dissolution, temperature-controlled mixing and low-temperature enzyme adding. The cleaning agent can effectively remove stubborn stains such as protein and fat on the surface of an endoscope (the removal rate is larger than or equal to 90%), the bacteriostasis rate on escherichia coli and staphylococcus aureus is larger than or equal to 99%, and corrosion to stainless steel, rubber and plastic materials is avoided. Experiments show that the enzyme activity retention rate of the multienzyme compound still reaches 80% or above after the multienzyme compound is stored for 2 years, and the technical problems that an existing cleaning agent is not thorough in decontamination, poor in antibacterial activity and capable of corroding equipment are solved.

The invention discloses a method for sterilizing a vegetable protein beverage, which comprises the following steps of: adding a composite antibacterial microsphere preparation into a vegetable protein beverage to be treated, uniformly mixing, and performing high-density CO2 treatment and magnetic induction electric field sterilization to obtain a vegetable protein beverage finished product, the composite antibacterial microsphere preparation is prepared by taking fucoidan and a gallic acid acylated quercetin-phospholipid conjugate as a substrate and loading lysozyme and hydroxytyrosol. High-density CO2 and a magnetic induction electric field are adopted for synergistic sterilization, so that the structure of a microbial film can be changed, metabolism is disturbed, a good matrix is provided for electric field sterilization, and the sterilization efficiency is further improved through electric breakdown, magnetic field refining of water molecular groups and heat effect; the composite microspheres are sensitive to an electric field, so that lysozyme and hydroxytyrosol can be triggered to be accurately and quickly released under the action of a magnetic induction electric field, stubborn spores can be efficiently killed, and the killing effect is improved by destroying cell wall structures and disulfide bonds of the spores; meanwhile, protein aggregation and flocculation in the beverage can be effectively prevented, and the stability of a beverage system is guaranteed.

The invention is applicable to the technical field of hydroxytyrosol preparation, and provides a method for preparing hydroxytyrosol by combining compound enzyme with molecular imprinting adsorption, which comprises the following steps: crushing fresh olive fruits, and collecting olive fruit pulp; putting the olive pulp into an enzymolysis tank, adding cellulose-pectin compound enzyme and citric acid, and performing enzymolysis; transferring the olive pulp subjected to enzymolysis into a microwave extraction tank, adding purified water, carrying out intermittent irradiation extraction, centrifuging after the extraction is completed, collecting supernate, and carrying out vacuum concentration; and adsorbing the concentrated supernate by using a chromatographic column filled with a hydroxytyrosol imprinted polymer, eluting with water and an ethanol solution in sequence, collecting ethanol eluent, and concentrating to obtain extract, namely the high-purity hydroxytyrosol. The problems that an existing fermentation method is low in yield and high in cost are solved. Generation of byproducts is reduced, and the separation and purification process is simplified; green and sustainable hydroxytyrosol industrial production is realized; and the purification period is shortened to be within 5 hours.

The invention provides an extraction method for increasing the oleuropein conversion rate and the hydroxytyrosol content in olive pomace, and belongs to the technical field of natural product extraction. Based on the analysis of the characteristics of the olive pomace material, firstly, the compact structure of the olive pomace is destroyed through steam explosion pretreatment, and pores are loosened; then carrying out compound enzyme enzymolysis, cooperatively hydrolyzing wrapped binding protein by adopting acid protease and hemicellulase in a combined manner, destroying a polysaccharide wrapping barrier in the pomace, and promoting rapid release of oleuropein; finally, high static pressure extraction is adopted, ultrahigh pressure is used for accelerating solvent permeation, the conversion rate of oleuropein is increased, and the content of hydroxytyrosol is comprehensively increased. The method is green and efficient, the oleuropein conversion rate can be increased to the maximum extent, and the content of hydroxytyrosol is increased; the degradation loss of hydroxytyrosol in the extraction process is reduced, and the economic benefits of the olive pomace are comprehensively improved.

Described herein are methods of treating a human child including administering to the human child a compound selected from epigallocatechin gallate (EGCG), or a derivative thereof selected from epicatechin (EC), epigallocatechin (RGC) and epicatechin gallate (ECG), sulforaphane, olive oil, phylloquinone, quercetin, safranal, hydroxtyrosol, menaquinone-4, menaquinone-7, oleocanthal, peptide Ins-1 B9-23 19CAM22R-E, peptide Ins-1 B12-23-19CAM, peptide Ins-1 B12-23 19CAM22R-E, or a combination of any of the foregoing, wherein administration of the treatment is started between birth and six months of age. Also described are methods of treating a pregnant human or a nursing human mother who has recently given birth to an infant. Further described are insulin B chain mimotope peptides.

The invention provides an olive leaf polyphenol component dynamic monitoring method and system based on principal component analysis, and relates to the technical field of computers, and the method comprises the following steps: obtaining original data; performing spectrum interval separation according to the original data to obtain a purified polyphenol characteristic spectrum sequence; performing spectrum deconvolution processing according to the purified polyphenol characteristic spectrum sequence to obtain a polyphenol subcomponent concentration evolution matrix; performing principal component reconstruction according to the polyphenol subcomponent concentration evolution matrix to obtain a reconstruction result; performing trajectory fitting according to a reconstruction result, and fitting a principal component score trajectory into a dynamic network model containing hydroxytyrosol glycoside hydrolysis and oxidative condensation steps to obtain a fitting result; and performing prediction according to the fitting result, predicting an evolution end point of the free-state content of hydroxytyrosol and the relative proportion of the hydroxytyrosol derivative, and obtaining a dynamic monitoring result. According to the method, the dynamic conversion process of specific polyphenol components such as hydroxytyrosol glucoside and oleuropein in the olive leaves is accurately monitored and predicted.

The invention discloses a synthesis method of hydroxytyrosol. The synthesis method comprises the following steps: (1) reacting p-hydroxyphenethyl alcohol as shown in a formula 1 with a bromination reagent in ethyl acetate at 0-25 DEG C to generate p-hydroxybromophenethyl alcohol as shown in a formula 2; (2) under the protection of nitrogen, reacting p-hydroxybromophenethyl alcohol in an aqueous solution of alkali in the presence of a copper catalyst and a ligand at 80-100 DEG C to generate hydroxytyrosol as shown in a formula 3; the copper catalyst is cuprous bromide; the ligand is phenanthroline. The raw materials and solvents required by the synthesis method are cheap and easy to obtain, the operation process is convenient and easy to implement, the synthesis method is environment-friendly, the reactant yield is high, the quality is good, and the synthesis method is suitable for industrial large-scale production.

The application discloses a preparation method of deodorizing anion dopamine modified graphene quantum dot cloud wool, and relates to the technical field of cloud wool.The hydroxyl tyrosol 3-sodium sulfate salt and sebacic acid are used in polyester polymerization reaction to prepare elastic polyester particles, so that the deformation resistance of the cloud wool is improved; meanwhile, the sulfate radical has the effects of antistatic and antibacterial; finally, the polyester chip is used as a core layer, the elastic polyester particles, dopamine modified graphene quantum dots and modified antibacterial agent are used as a skin layer, and melt spinning is carried out to prepare the deodorizing anion dopamine modified graphene quantum dot cloud wool, so that the effects of antibacterial, deodorization and antistatic are realized.The modified antibacterial agent uses 1,6-dibromoisoquinoline-3-amine and N,N,N',N'-tetramethyl-2-butene-1,4-diamine to form a double quaternary ammonium salt structure, so that the antibacterial effect of the cloud wool is improved, the elastic polyester particles are assisted, and the deodorization effect is improved; finally, tea polyphenol is added in the melt spinning process, a three-dimensional structure is formed, the skin layer protection capability is improved, and the deformation resistance is improved.

This invention provides a high-esterified persimmon pectin olive oil-based anti-fatigue beverage and its preparation method. The beverage comprises: extra virgin olive oil, high-esterified persimmon pectin, maltitol, freeze-dried peach powder, L-carnitine, vitamin E, hydroxytyrosol, and distilled water. The preparation process of this invention eliminates the need for processing aids such as emulsifiers and dispersants. High-esterified persimmon pectin is prepared through a pulsed electric field pretreatment-weak acid extraction process to obtain a highly active interfacial structure. This structure allows it to form a dense composite interfacial layer with other formulation components during two-stage shear emulsification, preferentially forming a stable oil-in-water emulsion system. This achieves long-term stable dispersion of the oil and water phases and masks the original spiciness and irritation of olive oil. Simultaneously, L-carnitine is added as a fatty acid metabolism support factor, and vitamin E and hydroxytyrosol are added as synergistic antioxidant components. The anti-fatigue effect is achieved through the synergistic effect of slow-release energy from the oil phase and interfacial structure regulation.

The invention belongs to the technical field of pet supplies, and particularly discloses a composition and application thereof in preparation of a product for reducing pet tear stains, the composition can effectively reduce the tear stains, and the composition, the composition and the product all have certain anti-inflammatory effects and jointly reduce tear secretion and inflammation-related tear stains. Meanwhile, the althaea rosea extract can improve the availability of hydroxytyrosol and fucoidan polysaccharide sulfate, improve eye microcirculation and relieve eye fatigue and dryness, fucoidan polysaccharide sulfate reduces iron elements entering tears through portal vein circulation and blocks generation of porphyrin-iron compounds, hydroxytyrosol improves metabolism and accelerates ferriporphyrin metabolism, and the effect of improving eye quality is achieved. The althaea officinalis extract promotes biliary secretion and accelerates ferriporphyrin metabolism, the synergistic interaction of the althaea officinalis extract, the althaea officinalis extract, the ferriporphyrin and the althaea officinalis extract can effectively reduce formation of tear stains, the risk of eye inflammation and bacterial infection is reduced, immunity can be improved, blood circulation can be promoted, and finally the pet is helped to keep a clean and healthy eye environment.

The application provides a co-crystal mixture for improving stability and antibacterial activity of hydroxytyrosol and a preparation method and application thereof, and belongs to the technical field of cosmetics and biological medicine. The co-crystal mixture is composed of the following chemical components in mass fraction: high-melting-point hydrogen bond acceptor: 20-95%, sulfur-containing strong reducing agent: 4-35%, and hydroxytyrosol: 5-60%; wherein the high-melting-point hydrogen bond acceptor includes high-melting-point amino acid or amino acid derivative. The co-crystal mixture can significantly improve the melting point of hydroxytyrosol, prevent or slow down the moisture absorption and deterioration of hydroxytyrosol, thereby reducing the transportation cost and storage cost of hydroxytyrosol. In addition, the co-crystal mixture can be configured into an aqueous solution preparation with different proportions, can be stored for a long time without discoloration in room temperature, refrigeration and high-temperature environments, and significantly improves the storage stability and biological activity of hydroxytyrosol.

The present invention relates to the field of pharmaceutical technology, specifically to hydroxytyrosol nicotinamide cocrystal, its preparation method and composition. The molar ratio of hydroxytyrosol and nicotinamide in the hydroxytyrosol nicotinamide cocrystal of the present invention is 1:1, and the cell parameters are a=9.4999, b=11.8285, c=11.4439, α=90°, β=96.628°, δ=90°. The hydroxytyrosol nicotinamide cocrystal of the present invention has a high melting point, is not hygroscopic, has good stability, and improves the convenience of use of hydroxytyrosol. The composition directly prepared by grinding method has a high yield, low cost, and is suitable for large-scale wide application.

Compositions are described including a synergistic combination of hydroxytyrosol and 3-0-acetyl-11-keto-B-boswellic acid. The hydroxytyrosol may be sourced from an olive extract and the 3-0-acetyl-11-keto-B-boswellic acid may be sourced from a Boswellia serrata extract. The compositions may be formulated for oral administration to a mammalian or an avian subject. Methods for treating, repairing, or reducing damage to connective tissue caused by one or more inflammatory mediators and for reducing levels of one or more inflammatory mediators in connective tissue are provided, the methods comprising orally administering the compositions to a subject in need thereof. 20 25 20 28 75 23 A pr 2 02 5 2 4 4 2 0 6 7 4 . 1 : D C C 3 1 / 0 5 / 2 0 2 3 C O M P O S I T I O N S C O M P R I S I N G H Y D R O X Y T Y R O S O L A N D B O S W E L L I C A C I D 2 0 2 5 2 0 2 8 7 5 2 3 2 0 2 5 A p r A B S T R A C T

Stabilized oils including an edible oil and an antioxidant composition comprising α-lipoic acid and least one of ascorbic acid, ascorbyl palmitate, green tea extract, lecithin, and rosemary extract or at least one of 1,2,4-benzenetriol, carnosic acid, dihydromyricetin, dihydrorobinetin, epigallocatechin, gallic acid, 3-hydroxytyrosol, myricetin, and nepodin, and methods of preparing such stabilized edible oils. The stabilized oils may have an Oxidative Stability Index (“OSI”) at 110° C. of at least 30 hours.

The invention provides a construction method and application of recombinant yarrowia lipolytica with high hydroxytyrosol yield, and belongs to the field of bioengineering. The recombinant Yarrowia lipolytica capable of producing the hydroxytyrosol at the high yield is named as a Yarrowia lipolytica XJ-HT strain, and after the recombinant Yarrowia lipolytica XJ-HT strain is subjected to fed-batch fermentation, the hydroxytyrosol can be synthesized at a relatively high level. The construction method of the recombinant yarrowia lipolytica is easy to operate, and efficient synthesis of hydroxytyrosol can be achieved.