64 results about "Nicotinamide riboside" patented technology

The invention relates to an anti-aging capsule compounded by PQQ and mitochondrial nutrients, and discloses an anti-aging capsule which is prepared by synergistically compounding a plurality of mitochondrial nutrients according to a scientific proportion and supplementing high-purity raw materials and slow-release dosage forms to realize energy metabolism activation and anti-oxidation synergistic intervention. Therefore, the anti-aging capsule systematically delays aging through multi-target regulation and control of mitochondrial functions. The capsule is characterized by comprising the following components in parts by weight: 1-20 mg of pyrroloquinoline quinone disodium salt (PQQ), 20-100 mg of coenzyme Q10, 50-200 mg of L-carnitine tartrate, 10-100 mg of alpha-lipoic acid, 50-300 mg of nicotinamide mononucleotide (NMN) or nicotinamide nucleoside (NR), and 10-50 [mu] g of selenoprotein or organic selenium yeast, and a capsule carrier comprises 100 mg of gelatin or a plant-derived capsule shell, 180 mg of microcrystalline cellulose, 5 mg of magnesium stearate and 5 mg of silicon dioxide.

The invention discloses a multi-system function regeneration factor composition as well as a preparation method and application thereof. The multi-system function regeneration factor composition is prepared from the following components in parts by mass: 0.8 to 2.4 parts of melatonin, 84 to 126 parts of nicotinamide ribose and 42 to 84 parts of resveratrol. According to the multi-system functional regeneration factor composition disclosed by the invention, through scientific proportioning and synergistic effect of melatonin, nicotinamide ribose and resveratrol, the functions of stem cells are strongly activated, proliferation of the stem cells is remarkably promoted, the stemness maintaining capability of the stem cells is improved, osteogenesis and adipogenesis differentiation potential is enhanced, and the regeneration effect of the stem cells is improved. Meanwhile, an NAD < + > metabolic pathway is activated so as to strengthen cell energy and anti-aging capability. The capsule adopts the design of combining quick-release pellets and sustained-release pellets, the quick-release part can quickly release melatonin, the local drug concentration can be increased in a short time, and stem cell activation signals can be quickly started; and the sustained release part slowly releases nicotinamide ribose and resveratrol to maintain the long-acting stability of the drug concentration, so that the effect taking speed is ensured, the action time is prolonged, and the bioavailability is greatly improved.

The invention belongs to the technical field of formula foods for special medical purposes, and particularly relates to a fat component for treating Alzheimer's disease and a preparation method of the fat component. The fat component is prepared from the following raw materials in parts by weight: medium-chain triglyceride, starch sugar, silicon dioxide, sodium caseinate, starch sodium octenylsuccinate, mono-glycerol fatty acid ester, diglycerol fatty acid ester and potassium hydroxide. And a neuroprotective nutrient combination. According to the invention, the medium-chain triglyceride capable of supplying energy quickly is creatively combined with the nerve protection nutrient with a specific mechanism. The medium-chain triglyceride provides efficient ketone energy; magnesium citrate stabilizes the excitability of neurons; the nicotinamide ribose supports cell energy metabolism; the omega-3 fatty acid plays roles in resisting inflammation and repairing nerve cell membranes. The four components provide protection from energy supply, neuron stabilization, metabolism support and structure repair, and bring better neurological function prognosis while controlling the Alzheimer's disease.

A composition containing nicotinamide mononucleotide (NMN) or an analog thereof, and certain antioxidants, more specifically resveratrol and at least one of ergothioneine and hydroxytyrosol, can be used in an anti-aging and / or anti-inflammatory method. The composition can contain NMN or nicotinamide riboside (NR) in an amount of from about 101 mg to about 1500 mg; the resveratrol in an amount of from about 50 mg to about 500 mg; ergothioneine or salt thereof, when present, in an amount of from about 3 mg to about 25 mg; and the hydroxytyrosol, when present, in an amount of from about 20 mg to about 100 mg.

The present application relates to the technical field of organic synthesis, and particularly relates to a preparation method of beta-nicotinamide mononucleotide. The present application provides a preparation method of beta-nicotinamide mononucleotide, comprising the following steps: mixing a salt corresponding to nicotinamide riboside, phosphorus oxychloride and a polar aprotic organic solvent, performing a phosphorylation reaction, and obtaining beta-nicotinamide mononucleotide. The present application takes nicotinamide riboside as a raw material, takes phosphorus oxychloride as a phosphorylation reagent, and is prepared through a phosphorylation reaction. Compared with a conventional method, the reaction has less by-products and is simple to operate. The subsequent high-purity and high-yield end product can be obtained through simple post-treatment. Meanwhile, the present application is also beneficial to industrial production.

The invention relates to an anti-aging composition, and belongs to the field of medicines and health-care product preparations. The composition is a microcapsule assembled by a water-soluble wall material, a first core material, a fat-soluble wall material and a second core material which are sequentially coated from outside to inside, and the first core material comprises beta-nicotinamide mononucleotide (NMN), nicotinamide ribose (NR), ergothioneine and trimethylglycine (TMG); the second core material comprises resveratrol, curcumin, pyrroloquinoline quinone (PQQ) and spermidine, and is dissolved in medium chain triglyceride (MCT oil) containing an antioxidant tocopherol. According to the dosage form, physical isolation and differentiated release of water-soluble and fat-soluble components are realized, and the problems of incompatibility among the components, poor stability, low bioavailability and the like are solved. Through the multi-target synergistic effect, the composition can effectively improve the NAD + level and enhance the mitochondrial function so as to improve the skin state and delay senescence.

The invention provides a moringa oleifera extract containing an anti-aging functional component nicotinamide ribose as well as a preparation method and application of the moringa oleifera extract. Belongs to the technical field of food and medicine. The invention provides the moringa oleifera extract containing a trace amount of natural anti-aging component nicotinamide ribose (NR) in fresh moringa oleifera seeds, and the moringa oleifera seed extract freeze-dried powder product contains 23.77 mu g / g of nicotinamide ribose, has anti-aging activity, and can be used for preparing new anti-aging drugs or anti-aging cosmetics or prepared into food. The invention also provides a simple and fast preparation technology of the moringa seed extract containing nicotinamide ribose (NR). The preparation method is high in operability, high in efficiency and high in yield.

The invention discloses application of nicotinamide ribose (NR) in preparation of anti-influenza virus products, and belongs to the technical field of biotechnology and medicine. The NR is used as a human endogenous metabolite, has excellent safety, activates SIRT1 by increasing the NAD + level in cells, and further inhibits influenza virus NP protein nucleus input and virus RNA synthesis. Experiments prove that NR has a remarkable inhibition effect on replication of various subtype influenza A viruses such as H1N1, H9N2 and H3N2, still has an inhibition effect under high-MOI infection, has a good use effect at the early stage of infection, and provides a new choice for anti-influenza virus drugs.

Provided are efficient heterologous expression of a nicotinamide ribokinase mutant in Escherichia coli and a use thereof. Provided are nicotinamide ribokinase capable of being heterologously expressed in Escherichia coli and a mutant thereof. The activity of the nicotinamide ribokinase of the mutant is significantly improved as compared with that of a parent. On the basis of the nicotinamide ribokinase and the mutant thereof, further provided is a method for the efficient heterologous expression of the nicotinamide ribokinase, that is, during the construction of a recombinant plasmid, by introducing a ribosome binding site into the plasmid, inserting a specific promoter sequence, etc., the enzyme activity of the expressed nicotinamide ribokinase is made higher than that of a control. In addition, further provided is a method for culturing a recombinant bacterium expressing the nicotinamide ribokinase, such that the highest enzyme activity of the expressed nicotinamide ribokinase is 208.3 U / mL. The present invention is conducive to efficient biosynthesis of β-nicotinamide mononucleotide and is suitable for industrial production and use.

The invention belongs to the technical field of biological catalysis, and particularly relates to an anchor peptide mediated fusion protein and an immobilized enzyme biofilm reactor, the fusion protein comprises a functional enzyme protein and an anchor peptide mutant; the anchoring peptide mutant is obtained by mutating proline at the fifth position of an amino acid sequence as shown in SEQ ID No.2 into valine; the functional enzyme protein is nicotinamide ribokinase or polyphosphate kinase. The functional enzyme protein and the anchoring peptide mutant are connected by using the connecting peptide, and then the obtained fusion protein is anchored on the polypropylene hollow fiber membrane wire material, so that the obtained fusion protein has good anchoring effect and catalytic activity, the batch reaction yield stability can be obviously improved, and the preparation method is suitable for industrial production. An immobilized enzyme bio-membrane reactor is designed, and nicotinamide mononucleotide can be synthesized through biological catalysis.

The invention discloses a synthesis method of nicotinamide ribose chloride and an intermediate thereof. The preparation method comprises the following steps: mixing chlorinated triacetyl ribose, nicotinamide, zinc chloride, tin tetrachloride and an organic solvent, and reacting to obtain triacetyl nicotinamide ribose chloride; or mixing tetraacetyl ribose, nicotinamide, zinc chloride, tin tetrachloride and an organic solvent, and reacting to obtain triacetyl nicotinamide ribose acetate; and adding the triacetyl nicotinamide ribose into an organic solvent solution of hydrogen chloride for deacetylation to obtain the nicotinamide ribose chloride. According to the method, the triacetyl nicotinamide ribose chloride is prepared by catalyzing the glycosylation of the chlorinated triacetyl ribose through the composite catalyst of zinc chloride and tin tetrachloride, so that the total yield, the product content and the purity are obviously improved; or a composite catalyst of zinc chloride and stannic chloride is used for catalyzing glycosylation of tetraacetyl ribose to prepare triacetyl nicotinamide ribose acetate, the purity is obviously improved, and further the preparation of nicotinamide ribose chloride also has obvious advantages.

The invention belongs to the technical field of biological catalytic synthesis, and particularly relates to a method for synthesizing beta-nicotinamide mononucleotide (NMN) by enzyme catalysis, which comprises the following steps: by taking D-ribose as a starting substrate, and utilizing a three-step enzyme catalysis reaction system which is synergistically completed by an S-methyl-5-thioribose kinase mutant (SMTK), purine nucleotidase (PNP) and nicotinamide ribose kinase (NRK), synthesizing beta-nicotinamide mononucleotide (NMN) by enzyme catalysis. And efficient synthesis of the NMN is realized. In order to further optimize the cost, an ATP (adenosine triphosphate) regeneration system is innovatively introduced in the process, so that the consumption of ATP in the reaction process is effectively reduced. On the basis, by combining an enzyme directed evolution technology, an immobilized enzyme technology and a continuous reactor design, the catalytic reaction efficiency and the product purity level are remarkably improved. The whole process has the characteristics of greenness and environmental protection, the reaction conditions are mild and controllable, the product yield stably reaches more than or equal to 85%, and the requirements of industrial large-scale production are completely met.

A stable intravenous (IV) composition comprises, in the intravenous form, nicotinamide riboside (NR) as described, or another nicotinyl riboside compound that is an NAD+ precursor. NR chloride may be provided in an intravenous (IV) formulation for administration to human subjects.

The present application aims to provide a composition having an effect of increasing NAD concentration. The present application relates to a composition characterized by containing one or more selected from nicotinamide riboside (NR), nicotinamide mononucleotide (NMN), and salts thereof and one or more sesamin as an effective ingredient.

A composition that exerts aging-delaying and anti-inflammatory effects by improving energy metabolism and mitochondrial function, a preparation method therefor, and use thereof. The composition comprises the following starting materials in parts by weight: 1-100 parts by weight of ergothioneine, 1-100 parts by weight of pyrroloquinoline quinone (PQQ) or a derivative thereof, and 20-1000 parts by weight of component C. The component C is β-nicotinamide mononucleotide (NMN), or nicotinamide riboside (NR) or a derivative thereof. Research has found that the combination of ergothioneine, PQQ or the derivative thereof, and NR or the derivative thereof or NMN can achieve a synergistic effect, with the anti-aging and anti-inflammatory effects being more significant than those of ergothioneine, PQQ or the derivative thereof, NR or the derivative thereof, or NMN alone.

The utility model discloses a pH regulation and control device of a nicotinamide ribose low-pH environment production line, and relates to the technical field of biochemical engineering production equipment.The pH regulation and control device comprises a detection cylinder, a production line pipeline and a water storage tank, a through hole is formed in the bottom of the detection cylinder, a closing mechanism is installed at the bottom of the inner side of the detection cylinder, and a liquid level sensor is fixedly connected to one side of the interior of the detection cylinder; pH value regulation and control mechanisms are mounted on the two sides of the detection cylinder; a connecting pipe is fixedly connected outside the production line pipeline, the water storage tank is fixedly connected to the upper side of the detection cylinder, and a cleaning mechanism is installed between the water storage tank and the detection cylinder. According to the device, a motor is adopted to drive a rotating disc to control opening and closing of a through hole, so that production line liquid automatically flows into a detection cylinder, the through hole is closed after a liquid level sensor is triggered for pH detection, stock solution is discharged back to a pipeline after detection, acid or alkali adjusting liquid is selected to be accurately injected to adjust the pH value, and after adjustment is completed, high-pressure water is sprayed to clean the inner wall of the detection cylinder; the influence of residues on subsequent detection is avoided, and efficient, accurate and self-cleaning pH regulation and control are realized.

The present application provides a fusion protein and its application in preparing a catalyst for producing nicotinamide mononucleotide, wherein the fusion protein comprises a nicotinamide riboside kinase (NRK) and an ATP recycling enzyme connected by a linker. The present application also provides a protein combination and its application, wherein the protein combination comprises the nicotinamide riboside kinase (NRK) and the ATP recycling enzyme in the fusion protein. The present application further provides a nucleic acid encoding the fusion protein or the protein combination, a recombinant expression vector containing the nucleic acid, a transformant containing the nucleic acid or the recombinant expression vector, and their applications. The present application also provides a method for efficiently preparing nicotinamide mononucleotide, which uses the fusion protein or the protein combination, and has the progress effects of high enzyme activity, high substrate concentration and high reaction efficiency.

The invention discloses a process research for separation and purification of nicotinamide riboside by a fermentation method, and the separation and purification of nicotinamide riboside (NR) is realized by the steps of membrane-passing impurity removal, nanofiltration pre-concentration, resin adsorption system treatment, nanofiltration concentration, alcohol precipitation crystallization and the like. By adopting the purification method, a pure NR product with the purity of 99% or above can be prepared from a crude NR product solution prepared by a fermentation method, and the yield is about 70%. The purification method has the advantages of simple and fast operation, no need of a large amount of toxic and harmful reagents, small environmental pollution and low purification cost, and is suitable for large-scale and large-scale industrial separation and purification of the NR product from the biological fermentation broth.

There are described compositions comprising an effective amount of a combination of two or more components, said components selected from acacetin, ACTI peptide, alpha-lipolic acid, alprostadil, anisomycin, apigenin, ascorbic acid, astragalus, berberine, β-lapachone, β-hydroxy-beta-methyl-butyrate, Bacopa monnieri, catechin, catechol, chamomile, chrysin, coumestrol, curcumin, dinitrophenol, dinoprost, ellagic acid, (−)-epigallocatechin gallate, green tea extract, fisetin, genistein, ginsenoside RE, glabridin, 18-α-glycyrrhetinic acid, 18-β-glycyrrhetinic acid, glycyrrhizin, hydroquinone, isoquercitrin (EMIQ), kaempferol, kuromanin, leucine, lithium, luteolin, luteolin, luteolinidin, melatonin, menadione, 1-methylnicotinamide (MNA), methyl salicylate, myricetin, nadide, niacin (vitamin B3), nicotinamide (NAM), nicotinamide mononucleotide (NMN), nicotinamide riboside (NR), nicotinic acid adenine dinucleotide (NaAD), nicotinic acid mononucleotide (NaMN), parsley (Petroselinium crispum), phenylephrine, pokeweed mitogen, 15-Δ prostaglandin J2, puromycin, quercetin, quinolinic acid, retinoic acid, trichostatin A, troxrutin, rutin, tryptophan, vitamin D3, withaferin A, wortmannin and zinc (including salts thereof).

The invention provides application of non-specific 5 '-nucleotidase in production of beta-nicotinamide ribose (NR), and belongs to the technical field of biology. The invention discovers that the non-specific 5 '-nucleotidase derived from specific bacteria has obviously better catalytic activity for catalyzing the hydrolysis of nicotinamide nucleotide (NMN) to generate beta-nicotinamide ribose (NR) compared with UshA derived from escherichia coli, and the 5'-nucleotidase can be used for large-scale industrial production of beta-nicotinamide ribose.

The invention discloses an efficient synthesis device of nicotinamide riboside chloride, and relates to the technical field of synthesis devices, the efficient synthesis device comprises a reaction cylinder, a lifting stirring assembly, a circulating flow guide assembly, a filtration detection assembly, a backflushing assembly, a stepped temperature adjustment assembly and a valve assembly. The filter detection assembly comprises a rotatable filter plate and a filter screen, and the crystal content is monitored in real time through the rotation amplitude of the filter plate; when crystallization reaches the standard, the linkage valve assembly automatically discharges materials, and meanwhile the step type temperature adjusting assembly adjusts the refrigeration intensity according to the crystallization process. The backflushing assembly can prevent the filter screen from being blocked, and the circulating flow guide assembly strengthens mixing of reaction liquid. The device solves the problems that a traditional device is delayed in crystallization monitoring, extensive in temperature control, insufficient in synthetic reaction and low in crystallization efficiency, realizes automatic control of the crystallization process, and remarkably improves the product purity and the production efficiency.

Methods and compositions for reducing symptoms of mental concentration dysfunction, mood dysfunction, and / or stress-related dysfunction. In various examples, the methods include selecting a subject experiencing a symptom of mental concentration dysfunction, mood dysfunction, and / or stress-related dysfunction and providing for consumption by the subject, a nicotinamide composition formulated to reduce a frequency and / or severity of the symptom. The nicotinamide composition includes a first component and a second component. In some examples, the first component includes nicotinamide (NAM), nicotinamide riboside, or nicotinamide mononucleotide and the second component includes one or more compounds that provide a choline cation and a succinate anion (2−). In certain examples, the molar ratio of the choline cation:succinate anion (2−):first component is between 2:1:0.01 and 2:1:1.

The invention provides a method of promoting weight management in a mammal comprising administering to the mammal a composition comprising at least one weight loss agent and at least one mitochondrial enhancer. The weight loss agent can be selected from the group consisting of psyllium, guar gum, capsaicin, chitosan, caffeine, gamboge, mume, capsaicin, yohimbine, butterfly pea, glucomannan, African mango, guarana, pyruvate, carnitine, beta-glucan, fucoxanthin, raspberry ketone, white kidney bean, kola nut, chromium, ginseng, psyllium, St. John's wort, dandelion, hydroxycitric acid, conjugated linoleic acid, green tea, black tea, coffee bean extract, forskolin, and bitter orange. The mitochondrial enhancer can be selected from the group consisting of beetroot extract, nitrate, idebenone, nicotinamide riboside, elamipretide, vitamin C, vitamin D, vitamin E, thiamine, riboflavin, magnesium, calcium, phosphate, phospholipid, creatine, pyruvate, coenzyme Q10, NADH, niacin, L-carnitine, dichloroacetate, curcumin, schisandrin, and resveratrol. Compositions for weight management are also provided.

The invention provides application of nicotinamide ribose in preparation of drugs for increasing the number of primordial germ cells and prolonging the growth cycle, and relates to the technical field of cell biology. Animal experiments prove that the supplement of nicotinamide ribose in the embryonic period can significantly increase the number of PGCs and prolong the post-birth growth cycle, and the action mechanism comprises: nicotinamide ribose promotes the proliferation of embryonic cells by up-regulating the NAD + level in PGCs, and enhances the expression of TFAP2C at the same time to maintain the undifferentiated state of cells and delay the meiosis initiation of female embryo PGCs and the differentiation initiation of male embryo PGCs; in post-birth effect evaluation, the number of follicles in ovaries of the female child rats in the NR group is remarkably increased, the fertility cycle is remarkably prolonged, the fertility of filial generations is not affected, and nicotinamide ribose is prompted to effectively maintain reproductive reserve and enhance the fertility by optimizing the development time sequence of PGCs. The invention provides a solid experimental foundation for the application of nicotinamide ribose in increasing the number of primordial germ cells and prolonging the growth cycle, and has a wide application prospect.

A composition for supporting nicotinamide adenine dinucleotide (NAD+) concentrations in humans is provided. The composition includes a synergistically effective amount of: nicotinamide riboside, nicotinamide and nicotinic acid; at least one of thiamin (vitamin B1), riboflavin (vitamin B2), pantothenic acid (vitamin B5), pyridoxine (vitamin B6), biotin (vitamin B7), folate (vitamin B9) and cobalamin (vitamin B12); and at least one of magnesium, trans-resveratrol and caffeine. Related methods are also provided.

A composition can treat or prevent at least one physical state selected from the group consisting of oxidative stress, a condition associated with oxidative stress, a reduced level of glutathione, and a condition associated with a reduced level of glutathione. The composition contains an effective amount of a combination of at least one glycine or functional derivative thereof, at least one N-acetylcysteine or functional derivative thereof, and at least one nicotinamide riboside or NAD+ precursor. The composition can be orally administered, for example as one or more of a food product, a food for special medical purposes (FSMP), a nutritional supplement, a ready to drink formula, a dairy-based drink, a low-volume liquid supplement, powder formats for liquid reconstitution or a meal replacement beverage.

Methods for stabilizing or encapsulating at least one compound selected from the group consisting of nicotinamide riboside (NR), nicotinic acid riboside (NAR), nicotinamide mononucleotide (NMN), nicotinic acid mononucleotide (NaMN), derivatives thereof, or salts thereof, are provided. Compositions including wax prills, stabilized, or encapsulated forms of at least one compound selected from the group consisting of nicotinamide riboside (NR), nicotinic acid riboside (NAR), and nicotinamide mononucleotide (NMN), derivatives thereof, or salts thereof, are also provided.

The present invention provides compounds and compositions containing reduced nicotinamide riboside for use in methods of promoting protective immunity and / or for preventing and / or treating bacterial or viral infection and / or for limiting immune-mediated pathology following infection in an individual, including delivering an effective unit dose of reduced nicotinamide to an individual in need thereof.

The present application relates to a kind of nicotinamide riboside kinase mutant, coding gene, vector containing coding gene, genetically engineered bacteria and application.The present application is aimed at wild type NrK, the three-dimensional structure of NrK is simulated by macromolecular modeling technology, and one or more sites possibly related to catalysis are predicted using energy minimum principle and molecular docking technology.The nicotinamide riboside kinase mutant constructed in the present application is compared with wild type enzyme, and enzyme activity is increased by 1.26-2.98 times, can significantly reduce the amount of enzyme, reduce fermentation volume and cost;And can greatly shorten reaction time, improve NMN yield, can meet the demand of large-scale industrial production of NMN prepared by biological enzyme method, has wide application prospect.

A nutraceutical composition, consisting of Nicotinamide Riboside in a range of 100-2000 mg or Nicotinamide Mononucleotide in a range 250-1000 mg, Spermidine in a range 0.5-10 mg, Rhodiola Rosea in a range 200-600 mg, Cacao in a range 1000-10,000 mg, L-Theanine in a range 50-200 mg, Glucosamine in a range 500-3000 mg, and Hyaluronic acid in a range 50-240 mg, or consisting of Epigallocatechin-3-gallate in a range 100-500 mg, Quercetin in a range 250-1000 mg, Resveratrol in a range 150-1000 mg, Sulforaphane in a range 1-50 mg, and Calcium Alphaketoglutarate in a range 300-2000 mg, or consisting of Microdose Lithium in a range 1-5 mg, Trimethylglycine in a range 500-2000 mg, Glutathione in a range 250-1000 mg, Fisetin in a range 100-1000 mg, Urolithin A in a range 250-2000 mg, Sodium Butyrate in a range 100-3000 mg, Berberine in a range 500-1500 mg, Apigenin in a range 50-500 mg, Cinnamon in a range 1000-6000 mg, and Malate in a range 1000-6000 mg.