146 results about "Luteolin" patented technology

The invention provides a metal polyphenol nanoparticle, a preparation method thereof and an application of the metal polyphenol nanoparticle in preparation of a medicine for treating acute lung injury, the metal polyphenol nanoparticle is formed by self-assembly of metal ions and polyphenol, the metal ions are selected from Mg < 2 + >, Zn < 2 + >, Mn < 2 + > or Cu < 2 + >, and the polyphenol is selected from Mg < 2 + >, Zn < 2 + >, Mn < 2 + > or Cu < 2 + >. The polyphenol is selected from epigallocatechin gallate (EGCG), caffeic acid (CA), chlorogenic acid (CGA), gallic acid (GA) and luteolin (LUT). The metal polyphenol nanoparticles are used for preparing a medicine for treating acute lung injury induced by sepsis, pneumonia, serious trauma or inhalation injury, and the medicine further comprises a pharmaceutically acceptable carrier. The metal polyphenol nanoparticles provided by the invention are simple in preparation process and high in biological safety, polyphenol and metal ions synergistically enhance the anti-inflammatory and antioxidant activity, and the metal polyphenol nanoparticles are superior to the single use of polyphenol or metal ions.

The invention discloses an application of luteolin in improving the reproductive performance of cocks. Researches show that in the breeding process of the cocks, the breeding performance of the cocks can be effectively improved by feeding the luteolin. Test data show that the luteolin can significantly improve the cock semen collection amount, improve the sperm density and sperm motility, significantly improve the diameter of a fine sperm tube and a spermatogenic cell matrix layer, and artificial insemination of the test cock semen improves the hatching egg fertilization rate, the hatching rate and the healthy chick rate. Therefore, the luteolin has important significance in research and development of related products for improving the reproductive performance of the cocks, and the luteolin has great potential in preparation of medicines, feeds or feed additives for improving the reproductive performance of the cocks.

The invention discloses a COS-O-luteolin derivative as well as a preparation method and application thereof. The COS-O-luteolin derivative is a compound obtained by modifying hydroxyl of chitosan oligosaccharide with luteolin. The preparation method comprises the following steps: weighing a certain amount of chitosan oligosaccharide, and dissolving the chitosan oligosaccharide in ethanol at 25 DEG C to obtain a chitosan oligosaccharide ethanol solution with the mass concentration of 0.5%; the method comprises the following steps: weighing quantitative luteolin, and dissolving the luteolin in ethanol at 25 DEG C to obtain a luteolin ethanol solution with the mass concentration of 0.5%; the preparation method comprises the following steps: mixing a chitosan oligosaccharide ethanol solution and a luteolin ethanol solution in equal volume, stirring for 2 hours, adjusting the pH value to 7.5, continuously stirring for 20-30 minutes, carrying out rotary evaporation at 45 DEG C to remove a solvent, carrying out vacuum drying at 60 DEG C, crushing and sieving to obtain the COS-O-luteolin derivative which can be applied to preparation of a fresh strawberry postharvest storage agent.

The invention discloses a multi-site mutant of glucosyltransferase and an application of the multi-site mutant. A GT 1 family O-glycosyl transferase UGT73C33 derived from liquorice is mutated, an excellent multi-site mutant of the O-glycosyl transferase is obtained through screening, the multi-site mutant contains three mutation sites, F148 is mutated into W, F388 is mutated into W, and A389 is mutated into T. The novel O-glycosyl transferase mutant C33-F148W-F388W-A389T is high in specificity, the selectivity of the novel O-glycosyl transferase mutant C33-F148W-F388W-A389T for generating luteolin-4 '-O-glucoside through specific conversion of luteolin reaches 80%, the novel O-glycosyl transferase mutant is high in catalytic activity, and the yield of luteolin-4'-O-glucoside reaches 156.8 mg / L under suitable conditions. The invention provides a unique biocatalyst and a conversion process thereof for the preparation of luteolin-4 '-O-glucoside with high activity and high added value.

The invention provides a fluorescent probe compound for simultaneously detecting HClO and ONOO <-> at two sites as well as preparation and application of the fluorescent probe compound. According to the probe compound, N, N-dimethylaminothioformyl ester is used as a response group of HClO, and the fluorescent probe RhB-ClO for simultaneously detecting HClO and ONOO <-> is constructed by expanding a conjugated structure of rhodamine. The probe shows excellent selectivity, high sensitivity (the detection limits are 9.9 nM and 13.1 nM respectively), good light stability and real-time responsiveness (the response time is 30 s and 150 s respectively) on HClO and ONOO <->. The probe not only can realize dual-channel imaging of HClO and ONOO <-> under oxidative stress conditions, but also can visualize the ferroptosis process of cells, a Parkinson's disease model and changes of HClO and ONOO <-> levels in an APAP-induced liver injury model. In addition, RhB-ClO can also be used for screening potential candidate drugs for nerve injury and liver injury, and it is proved that luteolin and piplongumine can be used as candidate antioxidant drugs.

The invention discloses a preparation method of prune exosomes and application of the prune exosomes in regulation and control of pigment metabolism and skin cell inflammatory response, and the preparation method comprises the following steps: taking prune, washing, peeling, dicing, juicing, adding a compound enzyme preparation, centrifuging at 2,000 g for 20 min, carrying out trehalose-assisted ultracentrifugation, centrifuging at 12,000 g for 30 min, centrifuging at 40,000 g for 60 min, centrifuging at 120,000 g for 70 min, collecting precipitate PBS, re-suspending, and filtering to obtain the high-purity prune exosomes. It is proved for the first time that the prune exosome dose-dependently inhibits the melanin content and tyrosinase activity in B16F10 melanoma cells, the mRNA expression level of key inflammatory factors TNF-alpha, IL-1beta and IL-6 in TNF-alpha-induced Hacat cells is remarkably reduced, in addition, the inhibition effect of melanin can be amplified through an STAT3 channel by combining the prune exosome with luteolin, and the application of the prune exosome to the preparation of the anti-inflammatory drug is promoted. The polypeptide can be used for preparing a preparation for regulating pigment anabolism and skin-related inflammatory response, and a potential biological application value is exerted.

The invention relates to the field of complex bone defect treatment, and discloses a nano material with an efficient antibacterial effect and a preparation method thereof.The preparation method comprises the steps that ammonium bismuth citrate and luteolin are mixed and dissolved in formamide, the mixture is put into a reaction kettle to react, a reaction solution is obtained, and Bi-Lu CDs in formamide is obtained after centrifugation and filtration; mixing the MSN-coated Bi-Lu CDs with amino-modified mesoporous silica microspheres, centrifuging, dispersing in deionized water, and drying in vacuum to obtain pure MSN-coated Bi-Lu CDs powder; the preparation method comprises the following steps: preparing a polyurethane dispersion liquid from polyurethane, spraying the polyurethane dispersion liquid on a quartz plate to form a polyurethane film, and spraying MSN and Bi-Lu CDs powder on the polyurethane film to carry out metal spraying treatment; a second precipitate is obtained; drying in vacuum to obtain J-Bi-LuCDs M (at) Au powder; the problems that in the prior art, due to the fact that a pure carbon dot material lacks active diffusion power, the treatment effect on complex tissue defects and bacterial biofilms is still limited, and a precursor material is poor in biocompatibility, not prone to dissolution and low in bioavailability are solved.

The invention discloses an ion locking fixed cyclodextrin cross-linked polymer functionalized cellulose-based textile material and a preparation method thereof, and the method comprises the following steps: cross-linking hydroxypropyl-beta-cyclodextrin (HP-beta-CD) by using 1, 2, 3, 4-butanetetracarboxylic acid (BTCA) to prepare a cross-linked polymer containing carboxyl / carboxylate groups, and carrying out cross-linking on the cross-linked polymer containing carboxyl / carboxylate groups to prepare the ion locking fixed cyclodextrin cross-linked polymer functionalized cellulose-based textile material. Polyphenols active molecules (such as luteolin) are clathrated and / or loaded; depositing the cross-linked polymer loaded with the active molecules on the surface of a cellulose-based textile base material which is subjected to cationization modification and has quaternary ammonium salt cationic groups on the surface through electrostatic interaction; divalent metal ions (preferably Ca < 2 + >) are further introduced to coordinate with carboxyl / carboxylate groups in the cross-linked polymer to form physical cross-linking points, so that locking and fixation of a functional layer are realized, and the release behavior of active molecules is regulated and controlled. The material has the comprehensive performance of stable fixation, washability, durability, breathability, comfort and maintenance and controllable release mode, and can be applied to application scenes such as skin contact textiles and dressing materials.

The invention discloses a liver targeting porous starch-luteolin compound and a preparation method and application thereof, and belongs to the technical field of drug delivery, corn porous starch is used as a carrier, and the compound with the liver targeting function is prepared through OSA hydrophobic modification, luteolin loading and epsilon-polylysine-galactoside targeting modification. The drug loading capacity and liver targeting property of luteolin are remarkably improved through triple modification, the production process is simple, the cost is low, and excellent blood sugar reducing and liver protecting effects are shown in diabetes liver injury treatment.

This invention belongs to the field of functional food fermentation technology, specifically relating to a method for fortifying peanut shells with luteolin and resveratrol through fungal fermentation, the resulting products, and their applications. The specific steps of this method are as follows: First, it was found that the relative contents of luteolin and resveratrol increased after co-fermentation of Ganoderma lucidum and peanut shells. Subsequently, by optimizing the fermentation and extraction conditions, the contents of resveratrol and luteolin in the fermented extract were increased. This method utilizes the bio-fermentation of edible and medicinal fungi, which utilizes components such as lignin in peanut shells to promote the growth of Ganoderma lucidum, and also releases luteolin and resveratrol, which are difficult to extract from peanut shells. The fermented extract contains both beneficial substances from Ganoderma lucidum and the two beneficial substances, luteolin and resveratrol, from peanut shells, with a 28.57% increase in resveratrol content and a 43.08% increase in luteolin content.

The invention discloses application of luteolin in reduction of indole production in ruminant rumen or in vitro. The invention also discloses a method for reducing or inhibiting the production of indole in a ruminant rumen or in an in-vitro fermentation system. It is found for the first time that luteolin can inhibit the activity of tryptophanase and reduce generation of indole. The invention has important application value in improving ruminant rumen microenvironment and reducing emission of malodorous substances.

This invention discloses a meglumine-luteolin complex, its preparation method, and its applications, belonging to the interdisciplinary field of microbiome and drug delivery. Using luteolin as the main material, this invention employs a dual inclusion technique with hydroxypropyl-β-cyclodextrin and meglumine to synergistically prepare a meglumine-luteolin complex with colon-targeted delivery capabilities. This invention significantly improves the delivery efficiency and drug loading of luteolin in the colon through the dual inclusion technique. The preparation process is simple, and it exhibits excellent hypoglycemic effects and colon-targeted release characteristics in the treatment of diabetes and its complications.

The invention relates to a qualitative and quantitative method for detecting active ingredients of root-strengthening and cough-relieving granules through UPLC-MS / MS, and belongs to the technical field of medicine analysis and detection. The four active ingredients of liquiritigenin, isoliquiritigenin, luteolin and bakuchiol in a sample can be detected at the same time. The method comprises the following steps: pretreating a sample by a 70% methanol ultrasonic extraction method to obtain a target sample solution, and detecting by using an SCIEX ExionLC AE high-pressure liquid phase system and an AB SCIEX 4500 mass spectrometer in combination with an MRM mode. Carrying out gradient elution on a liquid phase by using an HSS T3 C18 chromatographic column and 0.1% formic acid water-acetonitrile as a mobile phase; the mass spectrum is electrospray negative ion scanning, and a specific ion source and MRM parameters are set. The method can rapidly separate the four components, is short in detection period, high in sensitivity and strong in matrix interference resistance, can be accurately qualitative and quantitative, and provides support for preparation quality evaluation, pharmacodynamic mechanism research and clinical reasonable medication.

The utility model discloses accurate luteolin extraction equipment which comprises a plurality of functional units, firstly, raw materials are crushed by a material processing unit through a crushing mechanism, proper particles are screened out through a screening mechanism, and then, an extraction unit uses an ultrasonic-assisted extraction device to extract luteolin. Dissolving of luteolin by a solvent is accelerated through the cavitation effect of ultrasonic waves, then further extraction is conducted through a continuous countercurrent extractor, extracted liquid enters a separation and purification unit, solid impurities are rapidly separated through a high-speed refrigerated centrifuge, an extracting solution is precisely separated and purified through a liquid chromatography system, and the luteolin is obtained. Finally, the luteolin is subjected to final treatment through a filtering and drying unit, a membrane filtering system and a vacuum freeze dryer are combined for use, impurities are removed through ultrafiltration and nanofiltration technologies, and freeze drying is carried out under low-temperature and low-pressure conditions, so that degradation of heat-sensitive substances is avoided.

The application discloses a preparation method of an Fe-NC@CNFs / GCE electrode and application thereof, relates to the technical field of electrochemical detection, and has the technical scheme as follows: a bimetallic ZIF based on Fe / Zn is synthesized, and is calcined at a high temperature of 800 DEG C to obtain a derived nitrogen-doped porous carbon material (Fe-NC@CNFs); the synthesized material has a large specific surface area and rich active sites; the material is configured into a suspension by using ultrapure water; and a traditional drop-coating method is adopted to successfully build an electrode Fe-NC@CNFs / GCE for detecting luteolin; the electrode has high selectivity, sensitivity and stability, and can detect luteolin in a natural sample.

The invention discloses application of combination of ginsenoside Rb1 and luteolin in prevention and treatment of male reproductive toxicity caused by organophosphorus pesticides, and belongs to the field of medicines. A high-toxicity organophosphorus pesticide glyphosate is used as a representative, the reproductive toxicity effect of the glyphosate on male mice and the relieving effect of the ginsenoside Rb1 and the composition of the ginsenoside Rb1 and the luteolin on the male reproductive toxicity of the glyphosate are observed through tests, and the result shows that the ginsenoside Rb1 and the luteolin composition have the effect of inhibiting the reproductive toxicity of the glyphosate. The ginsenoside Rb1 and the composition of the ginsenoside Rb1 and the luteolin have a remarkable relieving effect on male reproductive toxic diseases caused by organophosphorus pesticides, wherein the male reproductive toxic diseases comprise testis diseases and sperm malformation rate increase, and antioxidant index GSH decrease and T-SOD increase caused by the organophosphorus pesticides. Effective drugs and treatment strategies are provided for preventing and treating organophosphorus pesticide induced male reproductive toxicity and diseases induced by organophosphorus pesticide induced male reproductive toxicity.

The present invention relates to a coloring composition comprising as a coloring agent a combination of: an extract A of the aerial parts of Lawsonia inermis containing between 7 and 60% by weight of lawsone in relation to the total weight of the dry extract, wherein the lawsone results especially from the enzymatic hydrolysis of glycosylated derivatives of lawsone, wherein said extract further comprises luteolin, apigenin and 2,3,4,6-tetrahydroxyacetophenone; and at least one other coloring agent B derived from a dye plant other than Lawsonia inermis, from microorganisms or from microalgae.

The application belongs to the technical field of chemical analysis and detection, and discloses a zinc oxide quantum dot fluorescent sensor and application of the zinc oxide quantum dot fluorescent sensor in detection of kaempferol. The zinc oxide quantum dot fluorescent sensor is prepared by stirring zinc salt solution and lye uniformly, then adding amino silane and water to carry out sol-gel reaction to prepare a ZnO-QDs fluorescent probe solution; then, a substrate is immersed in the ZnO-QDs fluorescent probe solution to carry out incubation, and drying is carried out to obtain the zinc oxide quantum dot fluorescent sensor. S The zinc oxide quantum dot fluorescent sensor is applied to detection of kaempferol, can produce specific response with kaempferol, makes the fluorescence intensity of the ZnO-QD fluorescent probe gradually increase with the increase of the concentration of kaempferol, and can effectively exclude the interference of structural analogs such as quercetin and luteolin, so that the kaempferol in the sample can be accurately quantified; and the detection cost is low, the operation is convenient, the detection efficiency is high, the detection is green and safe, and the zinc oxide quantum dot fluorescent sensor is suitable for popularization and application.

The invention discloses a water-soluble luteoloside derivative obtained by fermenting a probiotic composition as well as a preparation method and application of the water-soluble luteoloside derivative. The water-soluble luteoloside derivative is luteolin-3 '-alpha-7-beta-di-O-D-glucoside. The luteolin-3 '-alpha-7-beta-di-O-D-glucoside has the advantage that the luteolin-3'-alpha-7-beta-di-O-D-glucoside has

The invention relates to the technical field of medicine component content determination, in particular to a traditional Chinese medicine fingerprint spectrum detection method of a serpentgrass preparation, which finally determines a chromatographic column: Agilent color type: ZORBAX Eclipse Plus C18 4.6 * 250mm * 5mu m, a mobile phase A being 0.1% phosphoric acid water, a mobile phase B being methanol and a column temperature being 30 DEG C by combining repeated exploration and saving time and reagent cost; the sample size is 10 mu L, the chromatographic peak shape of each component is relatively good, the peak appearance time is stable, the linear relation, the precision, the repeatability and the stability are good, and the average recovery rate of luteolin in a sample of a sample adding recovery experiment is 95.2%; the method can be used for guiding feeding in the production and preparation process of the polygonum viviparum preparation and standardizing production operation, so that the integrity and controllability of quality control in the whole process are ensured, and the safety, effectiveness and stability of clinical medication are ensured.

The invention provides a multi-layer core natural polyphenol algal gel candy for pets with oxidation resistance. The algal gel candy comprises a skin material and a multi-layer core material wrapped in the skin material, the formula of the skin material comprises the following components in parts by weight: 80-90% of algae gel, 1-5% of coconut powder and 0.05-0.1% of potassium sorbate; the core material comprises an inner layer, a middle layer and an outer layer, each layer is prepared by mixing 1-3% of one of apigenin, quercetin and luteolin with 1-5% of coconut powder, and natural polyphenols contained in the inner layer, the middle layer and the outer layer are different from one another; according to the algae gel candy, multiple layers of natural polyphenols are used as core materials to be combined with algae gel used as skin materials, and the gel candy with the functional multiple layers of cores is prepared; the candy has relatively strong antioxidant ability, can effectively remove free radicals in the body and delay senescence so as to improve the immunity of the body, and has a wide market application prospect.

The invention discloses a keloid treatment gel material capable of reducing lactic acid generation, and belongs to the technical field of keloid treatment. The material is prepared by the following steps: firstly, carrying out esterification on 9-oxabicyclo [3.3. 1] nonane-2, 6-diol and S-allyl-L-cysteine to obtain an intermediate A, and then carrying out Schiff base reaction on the intermediate A and 5-methoxy heliotropin to obtain an intermediate B containing double bonds; then, copolymerizing with methylacryloylated chitosan and polyethylene glycol diacrylate under photo-initiation to form a cross-linked network structure; finally, active ingredients such as quercetin-3-O-glucuronide and luteolin-3 '-glucuronide are included, and auxiliary materials such as a humectant and a preservative are added, so that the composition is prepared. The gel has a slow release function, can effectively inhibit glycolysis, reduce lactic acid generation, scavenge free radicals and regulate inflammation and fibrosis microenvironment, has good biocompatibility and oxidation response drug release characteristics, and is suitable for prevention and treatment of keloids.

The application discloses a kind of Shenkun Yangxue granules fingerprint and its construction method, belong to traditional Chinese medicine detection technical field.Different batches of Shenkun Yangxue granules are prepared into test sample solution;Respectively with Danshensu, ephedrine hydrochloride, syringin, protocatechuic aldehyde, coffee acid, calycosin-7-O-β-D-glucoside, loganin, comphoric acid, shisonin, delphinidin and luteolin and kaempferol control preparation single control solution;Test sample solution and control solution are analyzed by high performance liquid chromatography and high resolution mass spectrometry respectively, 18 characteristic peaks are screened out, and 12 kinds of chemical components are identified by standard comparison and fragment information analysis, to obtain Shenkun Yangxue granules fingerprint.The Shenkun Yangxue granules fingerprint established by the method can be used for the quality control detection of Shenkun Yangxue granules, which is conducive to the comprehensive monitoring of product quality, and ensures the consistency, controllability and stability of product quality.

The present invention relates to a cosmetic composition comprising a carrot leaf fermentation extract fermented with lactic acid bacteria. Since the content of luteolin in the carrot leaf fermentation extract is high, the cosmetic composition with physiological and nutritional functions can be obtained. The cosmetic composition containing the carrot leaf fermentation extract of the present invention can exhibit excellent anti-inflammatory and lipid inhibition effects.

The invention discloses a mutant of flavone-5-O-glycosyl transferase ZmDo and application of the mutant of flavone-5-O-glycosyl transferase ZmDo. Glucose donor preference key amino acid site mutation is carried out on flavone-5-O-glycosyl transferase ZmDo obtained by structural domain recombination, namely, the 142 amino acid T is mutated into V. According to the novel flavone-5-O-glycosyltransferase mutant, the glucose donor preference is obviously changed, luteolin can be specifically converted into luteolin 5-O-xyloside, compared with a wild type, the conversion rate of the generated luteolin 5-O-xyloside is reduced to 30%, the conversion rate of the generated luteolin 5-O-xyloside is increased by more than 3 times, and the novel flavone-5-O-glycosyltransferase mutant has the advantages of high yield, high yield and the like. The yield under suitable conditions can reach 4450 mg / L, and the conversion efficiency reaches 90%. The novel xylose glycosyl transferase obtained by the invention is strong in specificity and high in directional conversion efficiency, and a non-natural flavone glycoside compound luteolin 5-O-xyloside and a preparation process thereof are created.

The invention discloses a preparation method of luteolin-loaded targeted polypeptide nanoparticles and application of the luteolin-loaded targeted polypeptide nanoparticles in hepatic fibrosis treatment, and belongs to the technical field of nano-drug delivery and liver disease treatment. The luteolin-loaded targeted polypeptide nanoparticles are used for treating or relieving liver fibrosis related diseases. According to the nanoparticle disclosed by the invention, a stable structure is formed through self-assembly of the amphiphilic polymer, the luteolin is physically entrapped, targeted delivery of liver immune cells is realized, and the nanoparticle shows good biocompatibility and safety in an in-vivo macrophage polarization and liver fibrosis model; the inflammatory response and collagenous fiber deposition can be remarkably inhibited, and the hepatic tissue fibrosis degree is improved. Compared with the existing anti-hepatic fibrosis medicine which has the problems of poor targeting property, low bioavailability, obvious side effect after long-term medication and the like, the luteolin-loaded targeting polypeptide nanoparticle has the remarkable advantages of high delivery efficiency, strong targeting property, good safety, stable treatment effect and the like.

Disclosed is an aptamer capable of binding to luteolin and other naringenin derived flavonoids, wherein the aptamer is: an RNA aptamer having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1 and SEQ ID NO: 2; or a DNA aptamer having a nucleotide sequence selected from the group consisting of SEQ ID NO: 3 and SEQ ID NO: 4; wherein for SEQ ID NO: 1 and 2, N is one nucleotide selected from the group consisting of A, G, C and U, and wherein for SEQ ID NO: 3 and 4, N is one nucleotide selected from the group consisting of A, G, C and T. Also disclosed is a riboswitch and a DNA expression cassette comprising the aptamer, a composition thereof, and a microbe containing the riboswitch or DNA expression cassette. Further disclosed is a method of detecting luteolin and other naringenin derived flavonoids produced in a microbe using the aptamer or riboswitch as disclosed herein.

The invention belongs to the technical field of medicines, and particularly relates to an active ingredient composition for treating xerophthalmia and muscae volitantes as well as a preparation method and application of the active ingredient composition. The composition is prepared from lycium barbarum polysaccharide, zeaxanthine, apigenin, luteolin, zinc gluconate, ophiopogon japonicus polysaccharide, ophiopogonin B, paeoniflorin and gallic acid according to a specific weight part ratio, all the active ingredients have a synergistic effect, the ocular surface microenvironment is improved, the vitreous opacity progress is inhibited, eye tissue damage is repaired, and the eye quality is improved. The synergistic treatment effect on the xerophthalmia and the muscae volitantes is achieved, and the symptoms such as eye dryness, eye acerbity, lacrimal secretion insufficiency, black shadow fluttering and blurred vision can be effectively relieved.

This invention belongs to the field of nanoparticle preparation technology, specifically relating to a luteolin nanoparticle preparation, its preparation method, and its application. The luteolin nanoparticle preparation comprises a liposome carrier and luteolin encapsulated therein. The liposome carrier includes lipids, cholesterol, and a co-mixing solubilization system composed of volatile terpene essential oils and a hydrophilic solubilizing component. Preferably, the hydrophilic solubilizing component is a nonionic surfactant with an HLB value of 10-17. By constructing a co-mixing solubilization system formed by premixing volatile terpene essential oils and a hydrophilic solubilizing component, the dispersion stability and encapsulation performance of luteolin in the lipid system are improved, resulting in a more uniformly distributed and stable nanoparticle preparation, which shows a trend towards improved lung delivery performance. Experimental results show that the luteolin nanoparticle preparation exhibits good intervention effects in a pulmonary nodular lesion model and shows a trend towards improved lung delivery performance.

This invention discloses a screening method for high luteolin-containing chrysanthemum tea varieties. During the peak flowering period, multiple healthy, disease-free plants of each variety are randomly selected, and their stem diameter, leaf weight, and number of petals are measured and averaged. The value is calculated using the following formula: Y = -0.387 + 0.268X6 + 0.001X7 + 0.02X3; where X3 is stem diameter, X6 is leaf weight, and X7 is the number of petals. The larger the Y value, the higher the luteolin content of the variety. This method can quickly and initially screen high-content varieties, has low identification costs, and is simple to operate. It is a rapid screening method suitable for early breeding stages and large-scale field evaluation.