515 results about "Luteolin" patented technology

The invention discloses a preparing method of total sweet chrysanthemum glycosides and total chromocor in sweet leaf chrysanthemum, which is characterized by the following: comprising sweet chrysanthemum glycosides, stevi primary glycosides, labroid glycosides A, B, C, D, E, F, duacl glycosides A and so on in total sweet chrysanthemum glycosides; comprising cyanidenon, meletin, cyanidenon-7-0-beta-D glycosides, celery element-7-0-beta-D-glycosides, quercetin, meletin-3-0-beta-D-arabinoside, meletin-3-0-[4-0-trans-coffe acyl-alpha-L-isodulcitol-(1-6)-beta-D-arabinoside] and so on; choosing one or several methods from solvent extraction, solvent extraction process, macroreticular absorption resin method, column chromatography, supercritical fluid chromatography, liquid-liquid counter-current partition chromatography and so on; extracting the total chromocor; setting content of sweet chrysanthemum glycosides element in total sweet chrysanthemum glycosides at 5-100%; counting 5-100% of all sweet chrysanthemum glycosides content with sweet chrysanthemum glycosides and labroid glycosides; counting 5-100% of chromocor element in sweet leaf chrysanthemum total chromocor; counting 5-100% of all total chromocor content with cyanidenon-7-0-beta-D glycosides, quercetin and meletin-3-0-[4-0-trans-coffe acyl-alpha-L-isodulcitol-(1-6)-beta-D-arabinoside].

The invention relates to a moldavica dragonhead extract, a moldavica dragonhead dropping pill and a production method thereof, wherein, the moldavica dragonhead extract contains higher total flavonoids and luteolin and the production method of the moldavica dragonhead extract is simply operated. Pharmacodynamics test result indicates that the moldavica dragonhead dropping pill acquired from the invention has excellent curative effect to rat ischemia myocardial injury; each dose group of the moldavica dragonhead dropping pill has varying degrees of protection; Composite salviae dropping pill has similar effect with the middle-dose group of moldavica dragonhead dropping pill; all dose groups of the moldavica dragonhead dropping pill have better curative effect than Yixing Badiranjibuya Keli at ischemia myocardial; dosages of middle-dose group of moldavica dragonhead dropping pill and low-dose group of moldavica dragonhead dropping pill are only 50 percent and 25 percent of the dosage of Yixing Badiranjibuya Keli respectively, thereby greatly improving the compliance of sufferers. The moldavica dragonhead dropping pill acquired from the invention is a novel preparation of convenient use and good compliance, thus bringing into play better clinical curative effect of the moldavica dragonhead, an age-old Uighur medicinal material. The production method of the moldavica dragonhead dropping pill can be carried out easily.

Flavonoids, especially luteolin, are shown to be effective against insulin dependent (Type I) and insulin independent (Type II) diabetes mellitus. It is demonstrated that luteolin works in mammals by binding and blocking the K_v1.3 potassium channel of T-cell and Beta cells. Antidiabetic and anti-autoimmune compounds can be selected by measuring their ability to bind to and block the K_v1.3 channel.

An anti-inflammatory composition for application to the nasal mucosa includes a combination of essential oils. The composition is intended to help prevent and/or alleviate the effects of exposure caused by the inhalation of pollutants and allergens. Preferred embodiments of the composition include jojoba oil, rosemary oil, any of a variety of citrus oils, coconut oil, sesame oil, soy oil, thyme oil, oregano oil, chamomile oil, peppermint oil, cardiospermum halicacabum, galphimia glauca, luffa operculata, bee's milk, bee's wax, and aloe vera in various combinations and sub-combinations. The composition may further include lauric acid, d-limonene and luteolin.

The invention discloses an effective part of stevia and the activity and the application thereof. The effective part mainly comprises stevioside category and flavone category which are obtained by extracting and separating from dried stevia leaves, wherein the sum of the percentage content of the stevioside components in the stevioside part is 5 to 100 percent (w/w), and the stevioside components mainly contains the stevioside, stevibioside, rebaudioside A, B, C, D, E, and F, dulcoside A and the derivative thereof, etc.; the sum of the percentage content of the flavone components in the flavone part is 5 to 100 percent (w/w), and the flavone components mainly contains luteolin, quercetin, luteolin-7-O-Beta-D-glucoside, apigenin-7-O-Beta-D-glucoside, quercitrin, quercetin-3-O-Beta-D-arabinoside, quercetin-3-O-(4-O-anti form-caffeoyl acyl-Alpha-L-rhamnose-(1 to 6)-Beta-D-galactoside) and the derivative thereof, etc., and 4, 5-dicaffeoylquinic acid and the derivative thereof, etc. The effective part has significant sugar-reducing and fat-reducing effects, can be used singly or combined with any other Chinese medicines and Western medicines or foods in any proportion, is used for preparing medicines or functional foods, and is used for treating hyperglycemia and hyperlipoidemia.

The invention aims to provide a material for preparing a molecular imprinted polymer by taking luteolin as a template and adopting a molecular imprinting technique method, and a method for separating and purifying the luteolin in a natural product, and belongs to the field of separation and purification of effective components from the natural product. The method comprises the following steps: (1) crude extraction, namely sequentially washing, drying, crushing and sieving peanut shells, extracting the peanut shell powder with 70 percent ethanol time by time under the assistance of ultrasonic waves, and concentrating and drying the extract; (2) preparation of the polymer, namely weighing the pure luteolin, a functional monomer, a cross-linking agent and an initiating agent in proportion, dissolving the materials in a solvent, performing ultrasonic processing, degassing and polymerization on the solution to obtain the block polymer, and sequentially grinding, sieving and eluting the block polymer to obtain the molecular imprinted polymer of the luteolin; and (3) purification, namely loading the prepared molecular imprinted polymer into a solid-phase extraction column, loading the crude extract, washing off the luteolin left on the column after removing impurities by eluting, concentrating and drying the luteolin to obtain the pure product of the luteolin.

Flavone magnetic molecularly imprinted polymer, preparation of the flavone magnetic molecularly imprinted polymer, and application of the flavone magnetic molecularly imprinted polymer to bamboo-leafflavone separation belong to the technical field of drug separation. Preparation of flavone magnetic molecularly imprinted polymer microspheres include subjecting nano Fe3O4 subjected to surface modification by oleic oil, template molecule luteolin, functional monomer 4-ethylpyridine and cross linking agent ethylene glycol dimethacrylate to suspension polymerization according to a template molecule, functional monomer and crosslinking agent ratio of 1:5-8:80. The flavone magnetic molecularly imprinted polymer microspheres are applicable to selective enrichment and purification of bamboo-leaf flavones in bamboo-leaf extract, and the preparation is simple, quick, high in recovery rate and the like and is widely applicable to the field of separation of natural flavone compounds.

The invention relates to a method for ultrasonically extracting and preparing flavonoids pigment from chrysanthemum, belonging to the field of food processing and especially used for deep processing chrysanthemum. The process comprises the following steps of: blanching, drying and hermetically storing chrysanthemum at a low temperature, filtering by using a 100-mesh sieve, soaking in 60% ethanol as an extraction solvent in a liquid-material ratio of 40/1 (v/w) for 2 h, ultrasonically extracting a dry base in a water bath at 70 DEG C for 10 min to acquire the yield of 13.34 percent; and evaporating in the water bath at 70 DEG C by using the 60% of ethanol extraction liquid in a rotating way to release an ethanol taste; and freezing and drying in vacuum, wherein analytical compositions reach 52.01% of the dry base and comprise flavonoids, linarin, flavonoids (luteolin, acacetin and apigenin), flavonols (quercetin), phenolic acid (chlorogenic acid and caffeic acid), metal elements, and the like. The invention provides a new method for deep processing the chrysanthemum and has strong maneuverability, good safety and good repetitiveness.

New compositions and methods for treating patients suffering from Amyotrophic Lateral Sclerosis (ALS) and other metabolic and autoimmune disorders, which include glycopeptides such as N-acetyl-D-glucosaminyl(β1-4)-N-Acetyl-muramyl-L-alanyl-D-isoglutamine (GMDP) and peptide analog-L-alanyl-D-glutamic acid (GMDP-A) of at least 98% purity administered either alone, or in combination with a flavone such as luteolin and/or an isoflavone such as genistein, optionally in combination with a flavonol glycoside such as isoquercitrin or rutin. The high purity glycopeptides have a decreased amount immunogenic impurities and demonstrate a synergistic effect when combined with luteolin and/or genistein in presence of isoquercitrin.

The invention discloses a construction method of a wild chrysanthemum flower UPLC fingerprint spectrum. The method comprises the following steps: crushing a wild chrysanthemum flower medicinal material, precisely weighing, adding methanol, performing ultrasonic treatment after weighing, filtering by use of a microfiltration membrane, and taking subsequent filtrate to obtain a test sample solution; respectively taking chlorogenic acid, galuteolin, luteolin and linarin, precisely weighing, and dissolving by use of the methanol to obtain a reference solution; and respectively measuring the test sample solution and the reference solution by use of an ultrahigh-efficiency liquid chromatogram system to generate chromatogram peaks of the test sample solution and the reference solution so as to generate the wild chrysanthemum flower UPLC fingerprint spectrum. The construction method of the wild chrysanthemum flower UPLC fingerprint spectrum related to the invention is simple, easy to operate and greatly improved in spectrum analytic capability, and has the advantages of improving the accuracy of results and greatly improving the stability and sensitivity of the detection of the fingerprint spectrum.

The invention relates to a method for extracting luteolin and beta-sitosterol from peanut shells. The method comprises the following steps: taking the peanut shells as materials, cleaning the peanut shells to remove impurities, crushing the cleaned peanut shells, performing extraction on the crushed peanut shells by an ethanol solution, carrying out filtration, decolorization and concentration under reduced pressure to obtain a luteolin and beta-sitosterol mixture, then mixing the luteolin and beta-sitosterol mixture with an alkali liquor, after that, carrying out extraction separation to obtain beta-sitosterol, performing extraction after pH adjustment to obtain a luteolin crude product, and performing alcohol dissolution, crystallization and recrystallization on the crude product to obtain a luteolin product with relatively high purity. The method is simple to operate, demands less on equipment, lowers the manufacturing cost of luteolin and reduces the risk of environmental pollution.

The preparation method of luteolin compound includes the following steps: adding raw material rutin or its derivative in water, adding NaOH under the condition of heating and stirring to completely dissolve raw material, cooling to room temperature, regulating pH to 2-6, filtering, wasing and drying to obtain raw material refined product; then adding solid alkali, prepared raw material refined product and Na2S2O4 into water according to mole ratio of raw material refined product; solid alkali: Na2S2O4: water=1:15-20:8-70:1110-3330, using 100-500 W microwave to heat and reflow of 0.25-2 hr, cooling to room temperature, regulating reaction liquor pH to 2-7, filtering, washing, drying, and using organic solvent to make recrystallization so as to obtain the invented product.

The present invention discloses 3-deoxyflavonoid compounds and methods for inhibiting T cell activity and treating diseases and disorders (eg, autoimmune diseases, inflammatory diseases, diabetes, ALS, MS, rheumatoid arthritis, etc.). In some cases, the potency and/or duration of action of luteolin and/or other 3-deoxyflavonoid compounds can be increased by administering these compounds together with rutin, rutin analogs, and/or rutin derivatives. Furthermore, in some cases, first-pass metabolism of luteolin or other 3-deoxyflavonoid compounds can be avoided by administering these compounds parenterally (eg, sublingually, bucally, intranasally, injection, etc.).

The invention relates to an anti-hepatitis B virus composition taken from fresh dandelion and an application of the anti-hepatitis B virus composition in preparation of an anti-hepatitis B virus drug and belongs to the field of traditional Chinese medicines. The anti-hepatitis B virus composition provided by the invention comprises a fresh dandelion polysaccharide component, a phenolic acid component and a flavone component and is characterized in that the weight ratio of the fresh dandelion polysaccharide component to the phenolic acid component to the flavone component is (2-40): (3-70): (2-50). According to an optimized scheme, the phenolic acid component takes caffeic acid, chlorogenic acid and isochlorogenic acid A as typical ingredients; the flavone component takes galuteolin, quercetin and luteolin as typical ingredients; and the weight ratio of the caffeic acid to the chlorogenic acid to the isochlorogenic acid A to the galuteolin to the quercetin to the luteolin is (0.25-10): (2-40): (0.25-20): (0.5-20): (0-10): (1.5-20). According to the invention, the fresh dandelion is taken as raw material, effective ingredients are separated, purified and enriched by macroporous resin, the effective ingredients are mixed to obtain the fresh dandelion anti-hepatitis B virus composition, a process is simple and convenient, related diseases caused by hepatitis B viruses can be effectively treated, cost is low, and mass production can be achieved.

The invention belongs to the technical field of medicine, and relates to a method for preparing flavonoids and total flavonoids in Linaria vulgaris and application thereof. The flavonoids and the total flavonoids come from flavonoid compounds in the Linaria vulgaris (including flavonoid compounds such as pectolinarin, aglycon, acetyl pectolinarin, acetyl linarin, acetyl linarin, hesperidin, robinin, hispidulin, luteolin, vanilla lignin, chrysin and the like). Dried whole herbs of the Linaria vulgaris are subjected to reflux extraction by water or alcohol, then a macroporous resin is used to send to a column, alcohols with different concentrations are used for elution, eluents are collected, then different elution flow ingredients pass through a silica gel chromatographic column, a polyamide chromatographic column, a gel chromatographic column and the like, to obtain various compositions, and the content of an obtained total flavonoid substance is more than 60 percent. The flavonoid compounds can be used for preparing medicines for treating angiocardiopathy, cough, and asthma. An extraction method of the invention is simple, practical and cheap, and is suitable for industrialized production.

A composite Chinese medicine for preventing and treating bacterial and viral diseases, inflammation and tumor, relieving pain and cough, decreasing blood fat, improving immunity, etc is proportionally prepared from luteolin and forsythia fruit or its extract. Its preparing process is also disclosed.

The invention discloses a callicarpa nudiflora effective part extract with a hemostatic effect and a preparation method of a pharmaceutical preparation of the callicarpa nudiflora effective part extract. The effective part extract and the pharmaceutical preparation contain total flavonoids of the callicarpa nudiflora and are extracted and separated from the callicarpa nudiflora which is a traditional Chinese medicine plant, the content of the total flavonoids is not less than 50%, and the effective part extract and the pharmaceutical preparation mainly contain galuteolin, luteolin-3'-O-beta-D-glucopyranoside, luteolin-4'-O-beta-D-glucopyranoside, calliterpenone, luteolin, apigenin and cosmosiin. The effective part extract and the pharmaceutical preparation have significant hemostatic effects.

A luteolin-metal salt for preventing and treating pulmonary fibrosis is prepared through adding rutin to the aqueous solution of sodium hydroxide, thermal reflux, cooling, regulating pH=1-4, filtering to obtain yellow solid, dissolving it in water, regulating pH=11-14, filtering, regulating pH=1-4, filtering, baking to obtain coarse product, refining in the mixture of water and methanol by heating, adding it to the solution of Na in absolute alcohol, reacting, and vacuum concentrating until it become solid. Its advantages are high purity and output rate, and low cost.

The invention relates to a preparation method of a boric-acid functionalized porous adsorbent, and belongs to the technical field of preparation of medicinal materials. The preparation method includes preparing boric-acid functionalized nano particles BA-MSNs, taking glycidyl methacrylate as a monomer, taking divinyl benzene as a cross-linking agent, taking azodiisobutyronitrile as an initiator, adding a surfactant Hypermer 2296, stirring to form Pickering high-internal-phase emulsion, and performing thermal-initiation polymerization to obtain boric-acid functionalized porous resin; introducing a B-N coordination compound according to a post-modification method to prepare the boric-acid functionalized porous adsorbent, and applying the boric-acid functionalized porous adsorbent to adsorption separation of luteolin under a neutral condition. The prepared adsorbent has a communicated porous structure, high permeability, high adsorption capacity and high recycle rate, and is environment friendly; adsorption can be achieved under the neutral condition, and secondary pollution and luteolin oxidation are reduced.

The invention relates to a pharmaceutical composition having alpha-glucosidase inhibition activity, wherein the pharmaceutical composition comprises a flavone compound and an alpha-glucosidase inhibitor, the flavone compound is at least one selected from a monomer such as baicalein, quercetin, luteolin, baicalein-7-O-glucoside and catechin, an organic salt of the monomer, and an inorganic salt of the monomer, and the alpha-glucosidase inhibitor is at least one selected from a monomer such as acarbose, voglibose and miglitol, an organic salt of the monomer, and an inorganic salt of the monomer. According to the present invention, the pharmaceutical composition can effectively reduce postprandial blood glucose, can inhibit the activity of alpha-glucosidase adopting starch, maltose and sucrose as substrates, and less uses the alpha-glucosidase inhibitor, such that the efficacy can be improved, the side effect of the alpha-glucosidase inhibitor can be effectively reduced, and hypoglycemia and other problems easily caused by drug combination are effectively solved.

The invention provides a method for extracting and purifying effective parts of peanut shells, and belongs to the technical field of natural medicine extraction. The purity of luteolin at the extracted effective parts of the peanut shells reaches 60%. The method specifically comprises the following steps: (1) carrying out extracting with an ethanol aqueous solution under micropressure; (2) concentrating; (3) performing recrystallization; (4) performing column chromatography. The greatest feature of the method is that the operation is simple and economical, requirements on instrument and equipment are low, the production period is short, and the method is suitable for industrial production; only ethanol and water which are used as reagents are adopted, so that the method is safe and reliable, and the obtained effective parts can be directly used for study of peanut shell efficacy or pharmacology.

The invention relates to a bamboo-leaf-flavonoids molecular imprinted solid-phase extraction column, preparation and application thereof, and belongs to the technical field of medicine separation. Template molecule luteolin, functional monomer 4-ethylpyridine and ethylene glycol dimethacrylate which is a cross-linking agent are polymerized into bamboo-leaf-flavonoids molecular imprinted polymer particles in a suspension manner, the molar ratio of template molecules to the functional monomer to the cross-linking agent is 1:(5-8):80. The prepared bamboo-leaf-flavonoids molecular imprinted polymer particles are filled in a polypropylene or glass solid-phase extraction column hollow tube, so that the bamboo-leaf-flavonoids molecular imprinted solid-phase extraction column is prepared. The bamboo-leaf-flavonoids molecular imprinted solid-phase extraction column is used for selectively gathering and purifying bamboo-leaf-flavonoids extracting solution, has the advantages if simplicity, rapidness, high recycling rate and the like, and is wide in application prospect in the field of separation of natural flavonoids compounds.

The invention relates to a clothing antibacterial softener preparation method, which comprises: weighing 5-10% by mass of dioctadecyl dimethyl ammonium chloride, 0.3-0.5% by mass of polydimethylsiloxane diquaternary ammonium salt, 7-15% by mass of luteolin, 2-10% by mass of ethylene glycol, and a proper amount of water, mixing, heating to a temperature of 55-60 DEG C, dissolving, heating to a temperature of 75 DEG C, adding 5-15% by mass of a thickening agent polyethylene glycol 6000, adding 0.5-1.0% by mass of eugenol, and cooling to obtain the product. According to the present invention, the luteolin is adopted as the main component so as to inhibit a variety of bacteria and viruses, such as staphylococcus aureus, escherichia coli, herpes simplex virus and poliovirus; the antibacterial function is strengthened under the premise of no influence on softening property and the like; and the clothing antibacterial softener with characteristics of good compatibility with the softener, stable performance, good antibacterial effect, capability of inhibition of odor due to bacterial breeding during a wearing process of clothing, no odor, no irritating, low toxicity, safety and no harm on environment is provided.

Belonging to the technical field of preparation of environment functional materials, the invention relates to a preparation method of a boron affiliated dual recognition molecularly imprinted material. The method includes: firstly modifying natural flake graphite powder to form graphene oxide, then taking graphene oxide (GO) as the matrix material to prepare an imprinted polymer (DR-MIPs) with dual recognition molecular imprinting function by atom transfer radical polymerization; and conducting a series of treatment to obtain an adsorbent, and applying the adsorbent to selective recognition and separation of luteolin in an aqueous solution. The boron affiliated dual recognition molecularly imprinted material prepared by the method provided by the invention has good thermal stability, large surface area, high adsorption capacity, the function of reversible adsorption/release of acid-base effect along with acidity and alkalinity, and significant LTL molecular recognition performance.