89 results about "Luteolin" patented technology

The invention relates to the field of complex bone defect treatment, and discloses a nano material with an efficient antibacterial effect and a preparation method thereof.The preparation method comprises the steps that ammonium bismuth citrate and luteolin are mixed and dissolved in formamide, the mixture is put into a reaction kettle to react, a reaction solution is obtained, and Bi-Lu CDs in formamide is obtained after centrifugation and filtration; mixing the MSN-coated Bi-Lu CDs with amino-modified mesoporous silica microspheres, centrifuging, dispersing in deionized water, and drying in vacuum to obtain pure MSN-coated Bi-Lu CDs powder; the preparation method comprises the following steps: preparing a polyurethane dispersion liquid from polyurethane, spraying the polyurethane dispersion liquid on a quartz plate to form a polyurethane film, and spraying MSN and Bi-Lu CDs powder on the polyurethane film to carry out metal spraying treatment; a second precipitate is obtained; drying in vacuum to obtain J-Bi-LuCDs M (at) Au powder; the problems that in the prior art, due to the fact that a pure carbon dot material lacks active diffusion power, the treatment effect on complex tissue defects and bacterial biofilms is still limited, and a precursor material is poor in biocompatibility, not prone to dissolution and low in bioavailability are solved.

The invention discloses an ion locking fixed cyclodextrin cross-linked polymer functionalized cellulose-based textile material and a preparation method thereof, and the method comprises the following steps: cross-linking hydroxypropyl-beta-cyclodextrin (HP-beta-CD) by using 1, 2, 3, 4-butanetetracarboxylic acid (BTCA) to prepare a cross-linked polymer containing carboxyl / carboxylate groups, and carrying out cross-linking on the cross-linked polymer containing carboxyl / carboxylate groups to prepare the ion locking fixed cyclodextrin cross-linked polymer functionalized cellulose-based textile material. Polyphenols active molecules (such as luteolin) are clathrated and / or loaded; depositing the cross-linked polymer loaded with the active molecules on the surface of a cellulose-based textile base material which is subjected to cationization modification and has quaternary ammonium salt cationic groups on the surface through electrostatic interaction; divalent metal ions (preferably Ca < 2 + >) are further introduced to coordinate with carboxyl / carboxylate groups in the cross-linked polymer to form physical cross-linking points, so that locking and fixation of a functional layer are realized, and the release behavior of active molecules is regulated and controlled. The material has the comprehensive performance of stable fixation, washability, durability, breathability, comfort and maintenance and controllable release mode, and can be applied to application scenes such as skin contact textiles and dressing materials.

This invention belongs to the field of functional food fermentation technology, specifically relating to a method for fortifying peanut shells with luteolin and resveratrol through fungal fermentation, the resulting products, and their applications. The specific steps of this method are as follows: First, it was found that the relative contents of luteolin and resveratrol increased after co-fermentation of Ganoderma lucidum and peanut shells. Subsequently, by optimizing the fermentation and extraction conditions, the contents of resveratrol and luteolin in the fermented extract were increased. This method utilizes the bio-fermentation of edible and medicinal fungi, which utilizes components such as lignin in peanut shells to promote the growth of Ganoderma lucidum, and also releases luteolin and resveratrol, which are difficult to extract from peanut shells. The fermented extract contains both beneficial substances from Ganoderma lucidum and the two beneficial substances, luteolin and resveratrol, from peanut shells, with a 28.57% increase in resveratrol content and a 43.08% increase in luteolin content.

The invention discloses application of luteolin in reduction of indole production in ruminant rumen or in vitro. The invention also discloses a method for reducing or inhibiting the production of indole in a ruminant rumen or in an in-vitro fermentation system. It is found for the first time that luteolin can inhibit the activity of tryptophanase and reduce generation of indole. The invention has important application value in improving ruminant rumen microenvironment and reducing emission of malodorous substances.

This invention discloses a meglumine-luteolin complex, its preparation method, and its applications, belonging to the interdisciplinary field of microbiome and drug delivery. Using luteolin as the main material, this invention employs a dual inclusion technique with hydroxypropyl-β-cyclodextrin and meglumine to synergistically prepare a meglumine-luteolin complex with colon-targeted delivery capabilities. This invention significantly improves the delivery efficiency and drug loading of luteolin in the colon through the dual inclusion technique. The preparation process is simple, and it exhibits excellent hypoglycemic effects and colon-targeted release characteristics in the treatment of diabetes and its complications.

The application discloses a preparation method of an Fe-NC@CNFs / GCE electrode and application thereof, relates to the technical field of electrochemical detection, and has the technical scheme as follows: a bimetallic ZIF based on Fe / Zn is synthesized, and is calcined at a high temperature of 800 DEG C to obtain a derived nitrogen-doped porous carbon material (Fe-NC@CNFs); the synthesized material has a large specific surface area and rich active sites; the material is configured into a suspension by using ultrapure water; and a traditional drop-coating method is adopted to successfully build an electrode Fe-NC@CNFs / GCE for detecting luteolin; the electrode has high selectivity, sensitivity and stability, and can detect luteolin in a natural sample.

The invention discloses application of combination of ginsenoside Rb1 and luteolin in prevention and treatment of male reproductive toxicity caused by organophosphorus pesticides, and belongs to the field of medicines. A high-toxicity organophosphorus pesticide glyphosate is used as a representative, the reproductive toxicity effect of the glyphosate on male mice and the relieving effect of the ginsenoside Rb1 and the composition of the ginsenoside Rb1 and the luteolin on the male reproductive toxicity of the glyphosate are observed through tests, and the result shows that the ginsenoside Rb1 and the luteolin composition have the effect of inhibiting the reproductive toxicity of the glyphosate. The ginsenoside Rb1 and the composition of the ginsenoside Rb1 and the luteolin have a remarkable relieving effect on male reproductive toxic diseases caused by organophosphorus pesticides, wherein the male reproductive toxic diseases comprise testis diseases and sperm malformation rate increase, and antioxidant index GSH decrease and T-SOD increase caused by the organophosphorus pesticides. Effective drugs and treatment strategies are provided for preventing and treating organophosphorus pesticide induced male reproductive toxicity and diseases induced by organophosphorus pesticide induced male reproductive toxicity.

The present invention relates to a coloring composition comprising as a coloring agent a combination of: an extract A of the aerial parts of Lawsonia inermis containing between 7 and 60% by weight of lawsone in relation to the total weight of the dry extract, wherein the lawsone results especially from the enzymatic hydrolysis of glycosylated derivatives of lawsone, wherein said extract further comprises luteolin, apigenin and 2,3,4,6-tetrahydroxyacetophenone; and at least one other coloring agent B derived from a dye plant other than Lawsonia inermis, from microorganisms or from microalgae.

The application belongs to the technical field of chemical analysis and detection, and discloses a zinc oxide quantum dot fluorescent sensor and application of the zinc oxide quantum dot fluorescent sensor in detection of kaempferol. The zinc oxide quantum dot fluorescent sensor is prepared by stirring zinc salt solution and lye uniformly, then adding amino silane and water to carry out sol-gel reaction to prepare a ZnO-QDs fluorescent probe solution; then, a substrate is immersed in the ZnO-QDs fluorescent probe solution to carry out incubation, and drying is carried out to obtain the zinc oxide quantum dot fluorescent sensor. S The zinc oxide quantum dot fluorescent sensor is applied to detection of kaempferol, can produce specific response with kaempferol, makes the fluorescence intensity of the ZnO-QD fluorescent probe gradually increase with the increase of the concentration of kaempferol, and can effectively exclude the interference of structural analogs such as quercetin and luteolin, so that the kaempferol in the sample can be accurately quantified; and the detection cost is low, the operation is convenient, the detection efficiency is high, the detection is green and safe, and the zinc oxide quantum dot fluorescent sensor is suitable for popularization and application.

The invention discloses a water-soluble luteoloside derivative obtained by fermenting a probiotic composition as well as a preparation method and application of the water-soluble luteoloside derivative. The water-soluble luteoloside derivative is luteolin-3 '-alpha-7-beta-di-O-D-glucoside. The luteolin-3 '-alpha-7-beta-di-O-D-glucoside has the advantage that the luteolin-3'-alpha-7-beta-di-O-D-glucoside has

The invention discloses a keloid treatment gel material capable of reducing lactic acid generation, and belongs to the technical field of keloid treatment. The material is prepared by the following steps: firstly, carrying out esterification on 9-oxabicyclo [3.3. 1] nonane-2, 6-diol and S-allyl-L-cysteine to obtain an intermediate A, and then carrying out Schiff base reaction on the intermediate A and 5-methoxy heliotropin to obtain an intermediate B containing double bonds; then, copolymerizing with methylacryloylated chitosan and polyethylene glycol diacrylate under photo-initiation to form a cross-linked network structure; finally, active ingredients such as quercetin-3-O-glucuronide and luteolin-3 '-glucuronide are included, and auxiliary materials such as a humectant and a preservative are added, so that the composition is prepared. The gel has a slow release function, can effectively inhibit glycolysis, reduce lactic acid generation, scavenge free radicals and regulate inflammation and fibrosis microenvironment, has good biocompatibility and oxidation response drug release characteristics, and is suitable for prevention and treatment of keloids.

The invention discloses a preparation method of luteolin-loaded targeted polypeptide nanoparticles and application of the luteolin-loaded targeted polypeptide nanoparticles in hepatic fibrosis treatment, and belongs to the technical field of nano-drug delivery and liver disease treatment. The luteolin-loaded targeted polypeptide nanoparticles are used for treating or relieving liver fibrosis related diseases. According to the nanoparticle disclosed by the invention, a stable structure is formed through self-assembly of the amphiphilic polymer, the luteolin is physically entrapped, targeted delivery of liver immune cells is realized, and the nanoparticle shows good biocompatibility and safety in an in-vivo macrophage polarization and liver fibrosis model; the inflammatory response and collagenous fiber deposition can be remarkably inhibited, and the hepatic tissue fibrosis degree is improved. Compared with the existing anti-hepatic fibrosis medicine which has the problems of poor targeting property, low bioavailability, obvious side effect after long-term medication and the like, the luteolin-loaded targeting polypeptide nanoparticle has the remarkable advantages of high delivery efficiency, strong targeting property, good safety, stable treatment effect and the like.

The invention belongs to the technical field of medicines, and particularly relates to an active ingredient composition for treating xerophthalmia and muscae volitantes as well as a preparation method and application of the active ingredient composition. The composition is prepared from lycium barbarum polysaccharide, zeaxanthine, apigenin, luteolin, zinc gluconate, ophiopogon japonicus polysaccharide, ophiopogonin B, paeoniflorin and gallic acid according to a specific weight part ratio, all the active ingredients have a synergistic effect, the ocular surface microenvironment is improved, the vitreous opacity progress is inhibited, eye tissue damage is repaired, and the eye quality is improved. The synergistic treatment effect on the xerophthalmia and the muscae volitantes is achieved, and the symptoms such as eye dryness, eye acerbity, lacrimal secretion insufficiency, black shadow fluttering and blurred vision can be effectively relieved.

This invention belongs to the field of nanoparticle preparation technology, specifically relating to a luteolin nanoparticle preparation, its preparation method, and its application. The luteolin nanoparticle preparation comprises a liposome carrier and luteolin encapsulated therein. The liposome carrier includes lipids, cholesterol, and a co-mixing solubilization system composed of volatile terpene essential oils and a hydrophilic solubilizing component. Preferably, the hydrophilic solubilizing component is a nonionic surfactant with an HLB value of 10-17. By constructing a co-mixing solubilization system formed by premixing volatile terpene essential oils and a hydrophilic solubilizing component, the dispersion stability and encapsulation performance of luteolin in the lipid system are improved, resulting in a more uniformly distributed and stable nanoparticle preparation, which shows a trend towards improved lung delivery performance. Experimental results show that the luteolin nanoparticle preparation exhibits good intervention effects in a pulmonary nodular lesion model and shows a trend towards improved lung delivery performance.

This invention discloses a screening method for high luteolin-containing chrysanthemum tea varieties. During the peak flowering period, multiple healthy, disease-free plants of each variety are randomly selected, and their stem diameter, leaf weight, and number of petals are measured and averaged. The value is calculated using the following formula: Y = -0.387 + 0.268X6 + 0.001X7 + 0.02X3; where X3 is stem diameter, X6 is leaf weight, and X7 is the number of petals. The larger the Y value, the higher the luteolin content of the variety. This method can quickly and initially screen high-content varieties, has low identification costs, and is simple to operate. It is a rapid screening method suitable for early breeding stages and large-scale field evaluation.

The application provides a metal polyphenol nanoparticle, a preparation method thereof and application thereof in preparing a medicine for treating acute lung injury. The metal polyphenol nanoparticle is self-assembled from metal ions and polyphenols. The metal ions are selected from Mg 2+ , Zn 2+ , Mn 2+ or Cu 2+ . The polyphenols are selected from epigallocatechin gallate EGCG, caffeic acid CA, chlorogenic acid CGA, gallic acid GA and luteolin LUT. The metal polyphenol nanoparticle is used for preparing a medicine for treating acute lung injury induced by sepsis, pneumonia, severe trauma or inhalation injury. The medicine further comprises a pharmaceutically acceptable carrier. The metal polyphenol nanoparticle provided by the application has the advantages of simple preparation process, high biological safety, synergistically enhanced anti-inflammatory and antioxidant activities of polyphenols and metal ions, and is superior to polyphenols or metal ions alone.

The invention belongs to the technical field of microbial fermentation, and particularly relates to saccharomycetes SY and application thereof in preparation of a codonopsis pilosula fermentation product which is high in in-vivo and in-vitro bioavailability and capable of improving gastrointestinal motility. The codonopsis pilosula fermented product is prepared by fermenting codonopsis pilosula with saccharomycetes SY. The content of lobetyolin in the pilose asiabell root leavening prepared by fermenting pilose asiabell root through the saccharomycetes SY is high, in-vitro digestion experiments prove that the removal rate of lobetyolin, total flavonoids and superoxide anion free radicals and the bioaccessibility of the total reducing capacity are remarkably improved, and rat experiments find that the pilose asiabell root leavening can promote gastrointestinal motility and improve the bioavailability of the pilose asiabell root. Intestinal mucosa regeneration and barrier repair are promoted; the content of effective components such as luteolin 7-glucosid-3 '-xyloside capable of enhancing immune cell activity, regulating inflammatory factor balance, protecting cells from oxidative stress injury and improving gastrointestinal digestion and absorption functions and energy metabolism in serum is increased, and absorption of the effective components in blood is promoted.

The invention is suitable for the technical field of biomedical materials, and provides a luteolin chemical grafting-based composite scaffold as well as a preparation method and application thereof. Polycaprolactone glycol, polyethylene glycol and luteolin form a high-molecular polymer through a chemical grafting method, and the high-molecular polymer is prepared into a stent form through 3D printing. The preparation method is simple in synthesis process and low in cost, and the luteolin chemically-grafted polycaprolactone glycol / polyethylene glycol high-molecular polymer composite scaffold is constructed through carbamate bonds, so that uniform dispersion and slow release of luteolin in the scaffold are realized; the problems that luteolin is poor in water solubility, high in metabolism speed and prone to agglomeration in physical blending are solved, immunoregulatory bone regeneration is achieved by means of the function that luteolin regulates and controls M2 type polarization of macrophages, and meanwhile the surface hydrophilicity of the polymer composite scaffold is improved. The composite scaffold can be applied to the biomedical field of bone defects in an immune microenvironment abnormal state and the like, and repair of a bone defect area is promoted through the effects.

The present application relates to the technical field of pharmaceutical compositions, and provides a composition for improving immune function, a preparation and a preparation method and application thereof, comprising: 25-40% of chlorogenic acid, 5-12% of luteolin, 10-20% of isochlorogenic acid A, 3-8% of caffeic acid, 0.5-1% of a targeting ligand, 2-5% of a microecological component, and 5-8% of a nano-carrier, and application of the composition in preparation of medicines, health products and functional foods for improving immune function, and a preparation method of the above-mentioned medicine preparation, comprising the following steps: preparing a core particle; coating the core particle with a sustained-release enteric coating to obtain a microcapsule particle; filling the microcapsule particle into a capsule shell to obtain an enteric capsule; coating the enteric capsule with a stomach-dissolving coating liquid to obtain a capsule preparation; and achieving the effect of improving the whole gastrointestinal immune function by rapidly targeting anti-inflammation in the stomach and continuously targeting regulation in the intestinal tract, and greatly improving the action efficiency and immune regulation effect of the preparation.

The invention relates to tripterine-luteolin-based self-assembled nanoparticles as well as a preparation method and application thereof, and belongs to the field of biological medicines. Compared with single tripterine, the tripterygium wilfordii derivative nano-particles provided by the invention have a stronger inhibition effect on tumor cells and lower toxicity, and have a good application prospect in the development of antitumor drugs.

The present application provides a kind of pharmaceutical composition with lipid-lowering effect, including luteolin, isorhamnetin, 9-hydroxy-4,7-megastigme-3-ketone, lotus leaf base, 3-O-feruloyl quinic acid, kaempferol-3-O-acacia disaccharide-7-O-glucoside, kaempferol-3-O-beta-D-acacia glycoside and ginsenoside Rg1.The composition of example 1 has lipid-lowering efficacy verified by golden hamster hyperlipidemia model and AML12, HepG2 cell fatty degeneration model, the regulation effect of the composition of example 1 on the key target points of AMPK signal pathway and NF-κB signal pathway is verified by using molecular pharmacology experiment, and the mechanism of the composition of example 1 in treating hyperlipidemia by regulating energy metabolism and anti-inflammatory mechanism is described.

The invention discloses a method for biosynthesizing luteolin or piceatannol by using CO2 and application of the method, and relates to the field of bioengineering. According to the invention, photosynthetic microorganisms such as blue-green algae are subjected to genetic modification, and a biosynthetic pathway of luteolin or piceatannol and a reducing power regeneration pathway represented by phosphite dehydrogenase are modularly assembled in the photosynthetic microorganisms; the obtained genetic engineering photosynthetic microorganism can directly and efficiently convert inexhaustible solar energy, greenhouse gas CO2 and phosphite into high-value luteolin or piceatannol, so that consumption of organic carbon sources of food crops and the like and complex steps and environmental pollution of traditional plant extraction or chemical synthesis are avoided. According to the method provided by the invention, the raw material cost is greatly reduced, the greenhouse gas CO2 can be absorbed, a promising technical approach is provided for replacing the traditional production mode depending on plant extraction, chemical synthesis or heterotrophic fermentation, and the method has important practical significance and industrial application value.

The application discloses the technical field of carbon dot application, and relates to a luteolin carbon dot for guiding lysosomes and a preparation method and application thereof. The luteolin carbon dot is prepared by using luteolin and 2,5-dihydroxyterephthalic acid as carbon source precursors through a hydrothermal method. The prepared luteolin carbon dot has a particle size of 0.5-4 nm, a fluorescence excitation wavelength of 360-440 nm, a maximum emission wavelength of 540 nm and a Zeta potential of -19 mv. Research shows that the luteolin carbon dot can guide macrophage and adipocyte lysosomes, promote M2 type macrophage polarization, and up-regulate the expression of the heat production and brown colorization marker gene Ucp1 of beige adipocytes. Further, the M2 type macrophage loaded with the luteolin carbon dot can promote the expression of the Ucp1 of the beige adipocyte through the release of the carbon dot and the paracrine effect, thereby increasing heat production and energy consumption. The application lays a foundation for developing an intervention and treatment scheme for obesity and related metabolic diseases based on the carbon dot in the future.

The invention discloses a method for extracting hypoglycemic active components of origanum vulgare through combination of enzymolysis, ultrasonic treatment and salting-out. According to the method, origanum vulgare raw materials are used as substrates, cell walls are destroyed through enzymolysis pretreatment, and the release efficiency of active ingredients is improved; extracting hypoglycemic active substances such as luteolin and the like by utilizing ultrasonic strengthening; primary purification is realized by adopting a salting-out mode; the content of aglycone is increased through acidic hydrolysis, and then multi-stage separation and purification are carried out to obtain high-purity active ingredients; and finally, the stability of the extract is improved by using a beta-cyclodextrin inclusion technology. The method is short in technological process, high in efficiency, capable of obtaining the high-activity extract with the luteolin content larger than or equal to 30%, and suitable for research and development of functional food, health care products and drugs.

The invention discloses a gingko GbHCT1 gene as well as an expression protein and application thereof, and belongs to the field of plant molecular biology. The GbHCT1 gene is obtained by analyzing and screening based on data of a ginkgo transcriptome in the earlier stage of a research group, the nucleotide sequence of the GbHCT1 gene is as shown in SEQ ID NO.1, and the amino acid sequence of expression protein of the GbHCT1 gene is as shown in SEQ ID NO.2. A GbHCT1 overexpression vector is constructed and the nicotiana benthamiana is transformed, so that the content of ten flavonoid substances such as luteolin and isorhamnetin-3-O-glucoside in the transgenic GbHCT1 tobacco is remarkably increased. KEGG enrichment shows that differential metabolites are mainly enriched in a flavone synthesis pathway. The application reveals that the ginkgo GbHCT1 gene has dual effects on synthesis of flavonoid compounds by regulating carbon source distribution of a phenylpropane metabolic pathway, and a theoretical basis, a gene resource and a method are provided for secondary metabolism regulation and genetic improvement of ginkgo.

The application provides a natural monomer composition and its application in hair blackening products, and belongs to the technical field of hair blackening. The composition comprises naringenin and luteolin in a mass ratio of 10:1-1:10. The two components have obvious synergistic effect on hair blackening, are suitable for being prepared into natural monomer products with simple formula, definite effective components, safety and effectiveness, hair blackening effect, and wide market prospect.

The invention provides a construction method and application of a specific chromatogram of Pinger honey cream. The construction method comprises the following steps: a) preparation of a reference substance solution: respectively adding methanol to dissolve caffeic acid, linarin, luteolin and apigenin as reference substances to obtain the reference substance solution; b) preparation of a test solution: mixing the Pinger honey paste with a methanol solution, performing heating reflux treatment or ultrasonic treatment, cooling, and filtering to obtain the test solution; and c) determining to obtain a high performance liquid chromatogram of the test solution and a high performance liquid chromatogram of the reference solution, and selecting a common peak from the high performance liquid chromatogram of the test solution by taking the high performance liquid chromatogram of the reference solution as a contrast to obtain the characteristic chromatogram of the Pinger honey cream. By adopting the method, the specific chromatogram of the Pinger honey cream can be obtained to characterize the quality of the Pinger honey cream, so that the stability of the medicinal materials is better evaluated, and a quality evaluation system of the Pinger honey cream is perfected.

The present application relates to a kind of construction methods of vinegar jinyinhua standard decoction HPLC characteristic map, the present application adopts high performance liquid chromatography, Kromasil 100-5C18 chromatographic column, with methanol and 0.05% phosphoric acid aqueous solution mixture as mobile phase gradient elution is carried out, under the condition that flow rate is 1.0mL / min, column temperature is 35 ℃, detection wavelength is 238nm, establish vinegar jinyinhua standard decoction HPLC characteristic map, chromatogram is matched by "Chinese medicine chromatographic fingerprint similarity evaluation system" (2004A edition) peak, 14 common peaks are defined, 6 characteristic peaks are identified as jinyinhua-5-O-madder glycoside, luteolin, tiliroside, apigenin, hydroxyl jinyinhua and jinyinhua, and the similarity of 21 batches of vinegar jinyinhua standard decoction characteristic map is evaluated, sample similarity result is above 0.95;The HPLC characteristic map analysis method of vinegar jinyinhua standard decoction constructed by the present application is simple, accurate, reproducible, provides methodological basis for the quality evaluation and control of vinegar jinyinhua formula granules system.

The invention discloses application of diosmetin and mendalin in prevention and treatment of sweet potato root rot, and belongs to the technical field of crop disease prevention and treatment. According to the application disclosed by the invention, the fact that the natural flavonoid compounds, namely diosmetin and mendalin, have obvious inhibitory activity on dominant pathogenic bacteria Fusarium foetens of sweet potato root rot is proved for the first time. The discovery not only provides a brand new compound choice for preventing and treating sweet potato root rot, but also widens the agricultural biological activity spectrum of the natural product, and opens up a new direction for the application of the natural product in the field of plant protection. According to the application disclosed by the invention, an in-vitro antibacterial experiment definitely shows that the diosmetin and the mendalin can effectively inhibit the growth of pathogenic bacteria hyphae of the sweet potato root rot at a relatively low concentration. Compared with conventional chemical agents, the two natural compounds derived from plants have the remarkable advantages of being good in environmental compatibility, not prone to generating residues, low in pathogenic bacterium drug resistance risk and the like, and the requirements of modern agriculture for sustainable development and ecological safety are met.

The invention belongs to but not limited to the technical field of plant protection, and particularly relates to application and method for improving wheat powdery mildew resistance through allogenic material treatment, 250 micromoles of luteolin is used for culturing powdery mildew spores, germination of the powdery mildew spores is remarkably inhibited, 500 micromoles of luteolin is used for pretreating wheat leaves, spraying is carried out once a day, spraying is carried out three times in total, and the yield of the wheat powdery mildew resistance is increased. After the leaves absorb the luteolin, powdery mildew is inoculated, and the result shows that the powdery mildew infection area of the wheat plants treated by the luteolin is remarkably reduced, and the number of powdery mildew spores on the leaves is remarkably reduced. The invention proves that the occurrence of wheat powdery mildew can be inhibited by exogenous luteolin treatment, and a certain reference is provided for research on a disease-resistant mechanism of powdery mildew and prevention and control measures.