213 results about "Luteolin" patented technology

The invention provides a metal polyphenol nanoparticle, a preparation method thereof and an application of the metal polyphenol nanoparticle in preparation of a medicine for treating acute lung injury, the metal polyphenol nanoparticle is formed by self-assembly of metal ions and polyphenol, the metal ions are selected from Mg < 2 + >, Zn < 2 + >, Mn < 2 + > or Cu < 2 + >, and the polyphenol is selected from Mg < 2 + >, Zn < 2 + >, Mn < 2 + > or Cu < 2 + >. The polyphenol is selected from epigallocatechin gallate (EGCG), caffeic acid (CA), chlorogenic acid (CGA), gallic acid (GA) and luteolin (LUT). The metal polyphenol nanoparticles are used for preparing a medicine for treating acute lung injury induced by sepsis, pneumonia, serious trauma or inhalation injury, and the medicine further comprises a pharmaceutically acceptable carrier. The metal polyphenol nanoparticles provided by the invention are simple in preparation process and high in biological safety, polyphenol and metal ions synergistically enhance the anti-inflammatory and antioxidant activity, and the metal polyphenol nanoparticles are superior to the single use of polyphenol or metal ions.

The invention relates to the technical field of nano-particles, and particularly provides a preparation method of luteolin-loaded mesoporous polydopamine nano-particles, which mainly comprises the following steps: adding mesoporous polydopamine nano-particles into a luteolin solution, stirring until luteolin is adsorbed onto mesoporous polydopamine, centrifuging, washing, and drying to obtain luteolin-loaded mesoporous polydopamine nano-particles. The luteolin-loaded mesoporous polydopamine nano-particles are obtained by adding the luteolin into the mesoporous polydopamine nano-particles; according to the luteolin-loaded mesoporous polydopamine nano-particles, the photo-thermal sterilization effect of the mesoporous polydopamine nano-particles is combined with the antibacterial effect of luteolin, so that the illumination sterilization effect at low temperature is achieved, and the luteolin-loaded mesoporous polydopamine nano-particles have an excellent antibacterial rate on staphylococcus aureus and escherichia coli.

The invention provides a preparation method of luteolin liposome as well as a product and application of the luteolin liposome, and relates to the technical field of liposome. The preparation method of the luteolin liposome comprises the following steps: S1, dissolving lecithin, cholesterol and luteolin in absolute ethyl alcohol according to a mass ratio of (40-60): 15: (4-6), and carrying out reduced pressure spin-drying to form a film; and S2, hydration: mixing the film with a phosphate buffer solution or water with the pH value of 6.8-7.2, stirring and hydrating at 50-60 DEG C for 6-24 hours to form a suspension, and performing probe ultrasonic treatment on the suspension. The obtained luteolin liposome has the advantages of high encapsulation efficiency, low particle size, high stability and good slow release effect. When being applied to cosmetics which can relieve skin and have no irritation effect on sensitive skin, the composition has a remarkable slow release effect.

The invention discloses a preparation method of a peony seed shell flavone extract, which comprises the following steps: extracting peony seed shell flavone (ZKHT) by adopting enzymolysis-ultrasonic extraction, and separating and purifying by using a resin adsorption combined membrane separation technology, so that various flavonoid active compounds such as kaempferol, luteolin and apigenin with high content in peony seed shells can be effectively extracted at the same time; the method provided by the invention provides technical support for reutilization of the peony seed shell waste, and realizes efficient and comprehensive utilization of the peony seeds and by-products thereof.

The invention provides application of luteolin in prevention of bone marrow-type radiation sickness, relates to the technical field of biomedicine, and provides application of luteolin in preparation of drugs for prevention and treatment of bone marrow-type radiation sickness. The invention discloses an application of luteolin in prevention or treatment of bone marrow type radiation sickness and radioactive hematopoietic system injury, and finds that the hematopoietic system injury caused by ionizing radiation can be slowed down by administration of luteolin before irradiation, specifically, peripheral hemogram is increased, and bone marrow injury is slowed down.

The invention provides a natural monomer composition and application thereof in hair-blacking products, and belongs to the technical field of hair blacking. The composition comprises naringenin and luteolin in a mass ratio of (10: 1)-(1: 10), the two components have an obvious synergistic effect on hair blackening, and the composition is suitable for being prepared into a natural monomer product which is simple in formula, exact in effective component, safe and effective and has the hair blackening effect and has a wide market prospect.

The invention discloses an application of luteolin in improving the reproductive performance of cocks. Researches show that in the breeding process of the cocks, the breeding performance of the cocks can be effectively improved by feeding the luteolin. Test data show that the luteolin can significantly improve the cock semen collection amount, improve the sperm density and sperm motility, significantly improve the diameter of a fine sperm tube and a spermatogenic cell matrix layer, and artificial insemination of the test cock semen improves the hatching egg fertilization rate, the hatching rate and the healthy chick rate. Therefore, the luteolin has important significance in research and development of related products for improving the reproductive performance of the cocks, and the luteolin has great potential in preparation of medicines, feeds or feed additives for improving the reproductive performance of the cocks.

The invention relates to the technical field of nano materials, and discloses a biomass carbon dot as well as a preparation method and application thereof. The biomass carbon dots are prepared from the following raw materials: aniline and flavonol compounds. According to the biomass carbon dot provided by the invention, flavonol compounds derived from biomass such as luteolin and peanut shells are taken as raw materials, nitrogen doping is carried out through aniline, and the biomass carbon dot has relatively good ultraviolet absorption capacity in UVA and UVB ranges and also has excellent visible light permeability and high-temperature ultraviolet aging resistance; the preparation method of the biomass carbon dots is simple in steps, raw materials are easy to obtain, and one-step synthesis can be achieved through a solvothermal method; the biomass carbon dots are doped on the basis of a common polyurethane film, the ultraviolet absorption capacity of the film in UVA and UVB ranges is remarkably improved, meanwhile, the visible light transmittance of the film is not affected, the weather resistance of the film is good, the film is suitable for the agricultural field, and normal photosynthesis can be guaranteed while ultraviolet damage to crops is reduced.

The invention discloses a COS-O-luteolin derivative as well as a preparation method and application thereof. The COS-O-luteolin derivative is a compound obtained by modifying hydroxyl of chitosan oligosaccharide with luteolin. The preparation method comprises the following steps: weighing a certain amount of chitosan oligosaccharide, and dissolving the chitosan oligosaccharide in ethanol at 25 DEG C to obtain a chitosan oligosaccharide ethanol solution with the mass concentration of 0.5%; the method comprises the following steps: weighing quantitative luteolin, and dissolving the luteolin in ethanol at 25 DEG C to obtain a luteolin ethanol solution with the mass concentration of 0.5%; the preparation method comprises the following steps: mixing a chitosan oligosaccharide ethanol solution and a luteolin ethanol solution in equal volume, stirring for 2 hours, adjusting the pH value to 7.5, continuously stirring for 20-30 minutes, carrying out rotary evaporation at 45 DEG C to remove a solvent, carrying out vacuum drying at 60 DEG C, crushing and sieving to obtain the COS-O-luteolin derivative which can be applied to preparation of a fresh strawberry postharvest storage agent.

The invention belongs to but not limited to the technical field of plant protection, and particularly relates to application and method for improving wheat stripe rust resistance through allogenic material treatment, 250 micromoles of luteolin is used for culturing stripe rust fungus spores, germination of the stripe rust fungus spores is remarkably inhibited, 500 micromoles of luteolin is used for pretreating wheat leaves, spraying is carried out once a day, spraying is carried out three times in total, and the yield of the wheat stripe rust resistance is increased. After the leaves absorb the luteolin, the puccinia striiformis is inoculated, and the result shows that the endogenous luteolin content of the wheat plants treated by the luteolin is increased, the infection area of the puccinia striiformis is remarkably reduced, the number of puccinia striiformis spores on the leaves is remarkably reduced, and the biomass of the puccinia striiformis is remarkably reduced. The invention proves that the occurrence of wheat stripe rust can be inhibited by exogenous luteolin treatment, and a certain reference is provided for stripe rust disease-resistant mechanism research and prevention and control measures.

The invention discloses a multi-site mutant of glucosyltransferase and an application of the multi-site mutant. A GT 1 family O-glycosyl transferase UGT73C33 derived from liquorice is mutated, an excellent multi-site mutant of the O-glycosyl transferase is obtained through screening, the multi-site mutant contains three mutation sites, F148 is mutated into W, F388 is mutated into W, and A389 is mutated into T. The novel O-glycosyl transferase mutant C33-F148W-F388W-A389T is high in specificity, the selectivity of the novel O-glycosyl transferase mutant C33-F148W-F388W-A389T for generating luteolin-4 '-O-glucoside through specific conversion of luteolin reaches 80%, the novel O-glycosyl transferase mutant is high in catalytic activity, and the yield of luteolin-4'-O-glucoside reaches 156.8 mg / L under suitable conditions. The invention provides a unique biocatalyst and a conversion process thereof for the preparation of luteolin-4 '-O-glucoside with high activity and high added value.

The present invention relates to a drug carrier system in the form of nanocapsules and nanospheres loaded with luteolin and oleuropein, which have a counteracting effect on tumour cell growth, invasion and angiogenesis, and to a production method (100) of this drug carrier system.

The invention provides a fluorescent probe compound for simultaneously detecting HClO and ONOO <-> at two sites as well as preparation and application of the fluorescent probe compound. According to the probe compound, N, N-dimethylaminothioformyl ester is used as a response group of HClO, and the fluorescent probe RhB-ClO for simultaneously detecting HClO and ONOO <-> is constructed by expanding a conjugated structure of rhodamine. The probe shows excellent selectivity, high sensitivity (the detection limits are 9.9 nM and 13.1 nM respectively), good light stability and real-time responsiveness (the response time is 30 s and 150 s respectively) on HClO and ONOO <->. The probe not only can realize dual-channel imaging of HClO and ONOO <-> under oxidative stress conditions, but also can visualize the ferroptosis process of cells, a Parkinson's disease model and changes of HClO and ONOO <-> levels in an APAP-induced liver injury model. In addition, RhB-ClO can also be used for screening potential candidate drugs for nerve injury and liver injury, and it is proved that luteolin and piplongumine can be used as candidate antioxidant drugs.

The invention discloses a copper ion-luteolin nano-composite and application thereof in treatment of inflammatory bowel disease, the obtained nano-composite has very strong stability and active oxygen scavenging ability, and has excellent anti-oxidation and anti-inflammatory ability, specifically, the nano-composite is negatively charged, can effectively target intestinal inflammation lesion, and can be used for treating inflammatory bowel disease, so that the copper ion-luteolin nano-composite can be applied to treatment of inflammatory bowel disease. Mucosal barrier repair is promoted, intestinal flora is regulated, and the inflammation microenvironment is remarkably regulated; the antioxidant and anti-inflammatory capacities of cells can be enhanced by regulating and controlling an oxidative stress pathway of a nuclear factor erythroid 2 related factor 2 / heme oxygenase-1 (Nrf2 / HO-1) and a nuclear factor kappa B (NF-kappa B) signal pathway; the compound shows a good treatment effect in a typical inflammatory bowel disease mouse model, including ulcerative colitis and Crohn's disease. The invention provides a new way for constructing a multi-layer treatment platform for treating the inflammatory bowel disease by using natural products.

The invention belongs to the field of preparation analysis, and discloses a method for establishing an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of detoxification and hematogenesis granules. According to the invention, a method of combining the HPLC fingerprint spectrum and the chemical mode of the detoxification and hematogenesis granules is established, the contents of the deacetylasperuloside, the rosmarinic acid, the scutellarin, the luteolin, the apigenin and the kaempferol in the detoxification and hematogenesis granules are detected at the same time, and a comprehensive quality evaluation model of the detoxification and hematogenesis granules with different batch numbers is established, so that the analysis result is comprehensive and objective; the method is simple, convenient, reliable, good in repeatability, accurate, stable and high in sensitivity, the multi-component content determination method provides scientific support for quality supervision of the Zhuang medicine preparation detoxification and hematopoiesis granules, and the method can be used for comprehensive quality evaluation and control of the detoxification and hematopoiesis granules. Therefore, the production stability of product quality and the safety and effectiveness of clinical medication are ensured.

The invention discloses a preparation method of prune exosomes and application of the prune exosomes in regulation and control of pigment metabolism and skin cell inflammatory response, and the preparation method comprises the following steps: taking prune, washing, peeling, dicing, juicing, adding a compound enzyme preparation, centrifuging at 2,000 g for 20 min, carrying out trehalose-assisted ultracentrifugation, centrifuging at 12,000 g for 30 min, centrifuging at 40,000 g for 60 min, centrifuging at 120,000 g for 70 min, collecting precipitate PBS, re-suspending, and filtering to obtain the high-purity prune exosomes. It is proved for the first time that the prune exosome dose-dependently inhibits the melanin content and tyrosinase activity in B16F10 melanoma cells, the mRNA expression level of key inflammatory factors TNF-alpha, IL-1beta and IL-6 in TNF-alpha-induced Hacat cells is remarkably reduced, in addition, the inhibition effect of melanin can be amplified through an STAT3 channel by combining the prune exosome with luteolin, and the application of the prune exosome to the preparation of the anti-inflammatory drug is promoted. The polypeptide can be used for preparing a preparation for regulating pigment anabolism and skin-related inflammatory response, and a potential biological application value is exerted.

The invention provides a quality detection method of antiviral particles. The quality detection method adopts HPLC-MS / MS (High Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) to simultaneously determine the following components: forsythiaside A, patchouli ketone, verbascoside, mangiferin, 5-methoxy-8-hydroxypsoralen, curcumenol, luteolin-7-glucuronide, kaempferol-3-O-rutinoside, baicalin, rutin and bergamot phenol. The detection method is a quantitative determination method for 11 active components in the antiviral particles, the detection method is short in time and low in cost, the quantitative analysis method is expected to provide reference for quality control of the antiviral particles, and the clinical curative effect and safety of the antiviral particles are guaranteed.

The invention relates to the technical field of carbon nanomaterials, particularly discloses a molybdenum-nitrogen co-doped carbon quantum dot, and also discloses application of the molybdenum-nitrogen co-doped carbon quantum dot. The molybdenum-nitrogen co-doped carbon quantum dot provided by the invention is synthesized by taking L-arginine, a molybdenum source and 1-pyrenecarboxaldehyde as precursors through a one-step hydrothermal method. The molybdenum-nitrogen co-doped carbon quantum dot provided by the invention has obvious advantages in detection of Cr2O7 < 2-> and luteolin, and can also be applied to fluorescent ink.

The invention relates to the field of complex bone defect treatment, and discloses a nano material with an efficient antibacterial effect and a preparation method thereof.The preparation method comprises the steps that ammonium bismuth citrate and luteolin are mixed and dissolved in formamide, the mixture is put into a reaction kettle to react, a reaction solution is obtained, and Bi-Lu CDs in formamide is obtained after centrifugation and filtration; mixing the MSN-coated Bi-Lu CDs with amino-modified mesoporous silica microspheres, centrifuging, dispersing in deionized water, and drying in vacuum to obtain pure MSN-coated Bi-Lu CDs powder; the preparation method comprises the following steps: preparing a polyurethane dispersion liquid from polyurethane, spraying the polyurethane dispersion liquid on a quartz plate to form a polyurethane film, and spraying MSN and Bi-Lu CDs powder on the polyurethane film to carry out metal spraying treatment; a second precipitate is obtained; drying in vacuum to obtain J-Bi-LuCDs M (at) Au powder; the problems that in the prior art, due to the fact that a pure carbon dot material lacks active diffusion power, the treatment effect on complex tissue defects and bacterial biofilms is still limited, and a precursor material is poor in biocompatibility, not prone to dissolution and low in bioavailability are solved.

The invention discloses an ion locking fixed cyclodextrin cross-linked polymer functionalized cellulose-based textile material and a preparation method thereof, and the method comprises the following steps: cross-linking hydroxypropyl-beta-cyclodextrin (HP-beta-CD) by using 1, 2, 3, 4-butanetetracarboxylic acid (BTCA) to prepare a cross-linked polymer containing carboxyl / carboxylate groups, and carrying out cross-linking on the cross-linked polymer containing carboxyl / carboxylate groups to prepare the ion locking fixed cyclodextrin cross-linked polymer functionalized cellulose-based textile material. Polyphenols active molecules (such as luteolin) are clathrated and / or loaded; depositing the cross-linked polymer loaded with the active molecules on the surface of a cellulose-based textile base material which is subjected to cationization modification and has quaternary ammonium salt cationic groups on the surface through electrostatic interaction; divalent metal ions (preferably Ca < 2 + >) are further introduced to coordinate with carboxyl / carboxylate groups in the cross-linked polymer to form physical cross-linking points, so that locking and fixation of a functional layer are realized, and the release behavior of active molecules is regulated and controlled. The material has the comprehensive performance of stable fixation, washability, durability, breathability, comfort and maintenance and controllable release mode, and can be applied to application scenes such as skin contact textiles and dressing materials.

The invention discloses a liver targeting porous starch-luteolin compound and a preparation method and application thereof, and belongs to the technical field of drug delivery, corn porous starch is used as a carrier, and the compound with the liver targeting function is prepared through OSA hydrophobic modification, luteolin loading and epsilon-polylysine-galactoside targeting modification. The drug loading capacity and liver targeting property of luteolin are remarkably improved through triple modification, the production process is simple, the cost is low, and excellent blood sugar reducing and liver protecting effects are shown in diabetes liver injury treatment.

This invention belongs to the field of functional food fermentation technology, specifically relating to a method for fortifying peanut shells with luteolin and resveratrol through fungal fermentation, the resulting products, and their applications. The specific steps of this method are as follows: First, it was found that the relative contents of luteolin and resveratrol increased after co-fermentation of Ganoderma lucidum and peanut shells. Subsequently, by optimizing the fermentation and extraction conditions, the contents of resveratrol and luteolin in the fermented extract were increased. This method utilizes the bio-fermentation of edible and medicinal fungi, which utilizes components such as lignin in peanut shells to promote the growth of Ganoderma lucidum, and also releases luteolin and resveratrol, which are difficult to extract from peanut shells. The fermented extract contains both beneficial substances from Ganoderma lucidum and the two beneficial substances, luteolin and resveratrol, from peanut shells, with a 28.57% increase in resveratrol content and a 43.08% increase in luteolin content.

The invention discloses application of luteolin in reduction of indole production in ruminant rumen or in vitro. The invention also discloses a method for reducing or inhibiting the production of indole in a ruminant rumen or in an in-vitro fermentation system. It is found for the first time that luteolin can inhibit the activity of tryptophanase and reduce generation of indole. The invention has important application value in improving ruminant rumen microenvironment and reducing emission of malodorous substances.

This invention discloses a meglumine-luteolin complex, its preparation method, and its applications, belonging to the interdisciplinary field of microbiome and drug delivery. Using luteolin as the main material, this invention employs a dual inclusion technique with hydroxypropyl-β-cyclodextrin and meglumine to synergistically prepare a meglumine-luteolin complex with colon-targeted delivery capabilities. This invention significantly improves the delivery efficiency and drug loading of luteolin in the colon through the dual inclusion technique. The preparation process is simple, and it exhibits excellent hypoglycemic effects and colon-targeted release characteristics in the treatment of diabetes and its complications.

The invention relates to a qualitative and quantitative method for detecting active ingredients of root-strengthening and cough-relieving granules through UPLC-MS / MS, and belongs to the technical field of medicine analysis and detection. The four active ingredients of liquiritigenin, isoliquiritigenin, luteolin and bakuchiol in a sample can be detected at the same time. The method comprises the following steps: pretreating a sample by a 70% methanol ultrasonic extraction method to obtain a target sample solution, and detecting by using an SCIEX ExionLC AE high-pressure liquid phase system and an AB SCIEX 4500 mass spectrometer in combination with an MRM mode. Carrying out gradient elution on a liquid phase by using an HSS T3 C18 chromatographic column and 0.1% formic acid water-acetonitrile as a mobile phase; the mass spectrum is electrospray negative ion scanning, and a specific ion source and MRM parameters are set. The method can rapidly separate the four components, is short in detection period, high in sensitivity and strong in matrix interference resistance, can be accurately qualitative and quantitative, and provides support for preparation quality evaluation, pharmacodynamic mechanism research and clinical reasonable medication.

The utility model discloses accurate luteolin extraction equipment which comprises a plurality of functional units, firstly, raw materials are crushed by a material processing unit through a crushing mechanism, proper particles are screened out through a screening mechanism, and then, an extraction unit uses an ultrasonic-assisted extraction device to extract luteolin. Dissolving of luteolin by a solvent is accelerated through the cavitation effect of ultrasonic waves, then further extraction is conducted through a continuous countercurrent extractor, extracted liquid enters a separation and purification unit, solid impurities are rapidly separated through a high-speed refrigerated centrifuge, an extracting solution is precisely separated and purified through a liquid chromatography system, and the luteolin is obtained. Finally, the luteolin is subjected to final treatment through a filtering and drying unit, a membrane filtering system and a vacuum freeze dryer are combined for use, impurities are removed through ultrafiltration and nanofiltration technologies, and freeze drying is carried out under low-temperature and low-pressure conditions, so that degradation of heat-sensitive substances is avoided.

The application discloses a preparation method of an Fe-NC@CNFs / GCE electrode and application thereof, relates to the technical field of electrochemical detection, and has the technical scheme as follows: a bimetallic ZIF based on Fe / Zn is synthesized, and is calcined at a high temperature of 800 DEG C to obtain a derived nitrogen-doped porous carbon material (Fe-NC@CNFs); the synthesized material has a large specific surface area and rich active sites; the material is configured into a suspension by using ultrapure water; and a traditional drop-coating method is adopted to successfully build an electrode Fe-NC@CNFs / GCE for detecting luteolin; the electrode has high selectivity, sensitivity and stability, and can detect luteolin in a natural sample.

The invention discloses application of combination of ginsenoside Rb1 and luteolin in prevention and treatment of male reproductive toxicity caused by organophosphorus pesticides, and belongs to the field of medicines. A high-toxicity organophosphorus pesticide glyphosate is used as a representative, the reproductive toxicity effect of the glyphosate on male mice and the relieving effect of the ginsenoside Rb1 and the composition of the ginsenoside Rb1 and the luteolin on the male reproductive toxicity of the glyphosate are observed through tests, and the result shows that the ginsenoside Rb1 and the luteolin composition have the effect of inhibiting the reproductive toxicity of the glyphosate. The ginsenoside Rb1 and the composition of the ginsenoside Rb1 and the luteolin have a remarkable relieving effect on male reproductive toxic diseases caused by organophosphorus pesticides, wherein the male reproductive toxic diseases comprise testis diseases and sperm malformation rate increase, and antioxidant index GSH decrease and T-SOD increase caused by the organophosphorus pesticides. Effective drugs and treatment strategies are provided for preventing and treating organophosphorus pesticide induced male reproductive toxicity and diseases induced by organophosphorus pesticide induced male reproductive toxicity.

The present invention relates to a coloring composition comprising as a coloring agent a combination of: an extract A of the aerial parts of Lawsonia inermis containing between 7 and 60% by weight of lawsone in relation to the total weight of the dry extract, wherein the lawsone results especially from the enzymatic hydrolysis of glycosylated derivatives of lawsone, wherein said extract further comprises luteolin, apigenin and 2,3,4,6-tetrahydroxyacetophenone; and at least one other coloring agent B derived from a dye plant other than Lawsonia inermis, from microorganisms or from microalgae.

A zein and luteolin composite material and its modified material and preparation method belong to the technical field of zein and luteolin composite materials. The zein and luteolin composite material is prepared by mixing zein and luteolin as raw materials, and its modified material is that the zein and luteolin composite material is treated with a high pressure field (high hydrostatic pressure or high pressure homogenization), and a new composite is formed through the interaction of the zein and luteolin composite system, and its thermal stability and solubility are increased, the surface hydrophobicity is reduced, and the antioxidant property is increased.