109 results about "Dicaffeoylquinic acid" patented technology

The invention relates to a method for preparing an antioxidative active extractive of sweet potato leaves, and belonging to the field of agriculture products processing. The method comprises the following steps: drying and crushing fresh sweet potato leaves; performing microwave assistant extraction after the decoloring and degreasing treatment is carried out; decompressing and concentrating extract in vacuum; chromatographing through a column, and absorbing by maroporous resin; collecting eluent; decompressing and concentrating the eluent in vacuum to obtain extract of the sweet potato leaves; and atomizing and drying the extract to obtain powdered extractive of the sweet potato leaves. The product is powdered, has the unique smell of the sweet potato leaves, contains 65 to 75 percent of total phenol, and contains chlorogenic acid and 3,5-O-dicaffeoylquinic acid. The method adopts mass waste sweet potato leaves to prepare the extractive with strong antioxidative activity, turns waste into wealth, is a green industry, has simple process, no need of larger equipment investment and high extracting rate of antioxidative active substances, and is applicable to industrial production.

The invention discloses an effective part of stevia and the activity and the application thereof. The effective part mainly comprises stevioside category and flavone category which are obtained by extracting and separating from dried stevia leaves, wherein the sum of the percentage content of the stevioside components in the stevioside part is 5 to 100 percent (w/w), and the stevioside components mainly contains the stevioside, stevibioside, rebaudioside A, B, C, D, E, and F, dulcoside A and the derivative thereof, etc.; the sum of the percentage content of the flavone components in the flavone part is 5 to 100 percent (w/w), and the flavone components mainly contains luteolin, quercetin, luteolin-7-O-Beta-D-glucoside, apigenin-7-O-Beta-D-glucoside, quercitrin, quercetin-3-O-Beta-D-arabinoside, quercetin-3-O-(4-O-anti form-caffeoyl acyl-Alpha-L-rhamnose-(1 to 6)-Beta-D-galactoside) and the derivative thereof, etc., and 4, 5-dicaffeoylquinic acid and the derivative thereof, etc. The effective part has significant sugar-reducing and fat-reducing effects, can be used singly or combined with any other Chinese medicines and Western medicines or foods in any proportion, is used for preparing medicines or functional foods, and is used for treating hyperglycemia and hyperlipoidemia.

This invention relates to the new use of dicaffeoylquinic acid derivatives for treating Hepatitis B and diseases associated with retrovirus (such as HIV), the new caffeoylquinic acid derivatives and the composition containing the same.

The invention discloses a method for preparing a high purity dicaffeoylquinic acid compound by high-efficient separation, mainly comprising: using an alcohol-water solution to extract taraxacum fresh plant or dry plant, carrying out the column chromatographic separation after the concentration, preparing a crude extract rich in dicaffeoylquinic acid compound, then isolating and purifying the crude extract by high-speed countercurrent chromatography, and finally adopting a recrystallization process for refining so as to obtain 3,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid with high purity. The method is applied to the preparation of high purity monomers by taking various natural products or natural product extracts containing one or a plurality of ingredients in the dicaffeoylquinic acid compound and acquired through various approaches as raw materials, and has advantages of simple steps, simple operation, high efficiency, low cost, easy repetition, and large output of separation and preparation. The purity of dicaffeoylquinic acid compound prepared by the method can reach 98 percent.

The invention discloses a separation purifying method for a DCQA (dicaffeoylquinic acid) component in jerusalem artichoke, belonging to the field of effective composition developing and utilizing. The main processing steps for extracting and separating a chemical compound in jerusalem artichoke are as follows: dried medicinal materials of jerusalem artichoke are crushed, ultrasonic or backflow extraction is carried out by ethanol (50 to 80%) in the quantity of 10 to 20 times, petroleum ether is degreased, extraction is carried out by ethyl acetate, and extraction liquid is decompressed and concentrated into an extractum. The extractum is preliminarily purified by resin or silica gel column chromatography and is further purified by SephdexLH20 so as to obtain the DCQA component with the content of higher than 85%. With simple technique and easy operation, the separation purifying method adopts diversified column chromatography to separate and purify the chemical compound of DCQA in jerusalem artichoke; the content of DCQA in a finished product is high, the source of raw materials is rich, the resin or SephdexLH20 can be recycled, and the industrialization is easy.

The invention relates to a method for separating and purifying chlorogenic acid and 3,5-dicaffeoylquinic acid from a honeysuckle flower. According to the method, the honeysuckle flower is taken as a raw material and the method comprises the following steps: (1) preparing a honeysuckle flower crude extract; (2) extracting; (3) separating and purifying through macroporous adsorption resin; (4) separating and purifying through a semi-preparation type high efficiency liquid chromatography, namely separating and purifying by adopting the semi-preparation type high efficiency liquid chromatography to obtain two high-purity components by taking methanol-water as a mobile phase, wherein the two high-purity components are respectively identified as the chlorogenic acid and the 3,5-dicaffeoylquinic acid. The process disclosed by the invention is environment-friendly in process without severe harm on the environment and is low in integrated cost.

The invention provides an application of dicaffeoylquinic acid for the preparation of drugs of cerebral neuroprotection and the drugs can be used for curing ischemic strokes. The invention also provides a drug composite of cerebral neuroprotection. Drug effect tests and researches show that dicaffeoylquinic acid component is a new kind of effective ingredients which can protect cerebral nerve better, can both promote the survival of cerebral cortical neurons in vitro and the antioxidation and has a certain function of passing blood-brain barrier.

The invention relates to a method for preparing a dicaffeoylquinic acid methyl compound and an anti-influenza and antihepatitic medicine composition thereof. The method for preparing a dicaffeoylquinic acid methyl compound has the following steps of: extracting from woodbine, separating and modifying to obtain the dicaffeoylquinic acid methyl compound; and preparing the anti-influenza and antihepatitic medicine composition thereof. A great amount of anti-influenza and antihepatitic medicine composition containing dicaffeoylquinic acid methyl compound used as effective ingredients can be produced from plants with low dicaffeoylquinic acid methyl compound content by the invention.

A dicaffeoylquinic acid-containing beverage of the present invention includes the following components (A) and (B): (A) 0.02 to 0.18 mass % of dicaffeoylquinic acids; and (B) 0.1 to 1.0 mass % of L-arginine, in which a mass ratio between the component (A) and the component (B), [(B)/(A)], is from 2 to 18.

The present invention relates to the preparation of a Cynara scolymus extract obtainable by fractioning on a resin. The process of the invention allows to obtain an extract, starting from the aerial parts of the plant Cynara scolymus, containing three classes of active principles, namely dicaffeoylquinic acids, luteolin and cynaropicrin glycosides, in a constant ratio. Cynaropicrin is stabilized by addition of precise amounts of sulfated amino acids or suitable thio-derivatives. These extracts have hypolipemizing, anti-dyspeptic and vascular anti-inflammatory activities. The extracts are mainly formulated in Enothera biennis oil or in oils rich in ω-3 and ω-6 acids which enhance the vascular activity.

The invention relates to a method for preparing a phenolic acid compound through fatsia japonica. According to the method, the phenolic acid compound is obtained with fatsia japonica as a raw material through extraction and separation or obtained through biological conversion of aspergillus niger and eurotium after extraction and separation. According to the method, the phenolic acid compound in fatsia japonica is researched and quantitatively analyzed for the first time, and the total amount of the phenolic acid compound in fatsia japonica is 0.279-1.38%. Besides, the yield of a dicaffeoylquinic acid compound is increased through the biological conversion method, and a new raw material and a new method are provided for obtaining the phenolic acid compound.

The invention relates to the technical field of medicine detection, and in particular relates to a phlegm-heat clearing injection fingerprint spectrum establishment method and a fingerprint spectrum thereof; the phlegm-heat clearing injection is prepared from scutellaria baicalensis, bear gall powder, cornu gorais, honeysuckle and fructus forsythiae, and a phlegm-heat clearing injection fingerprint spectrum is established by detecting the phlegm-heat clearing injection components by adopting an ultra-high performance liquid chromatography method; the method specifically comprises the followingsteps of S1, preparing a reference substance solution: taking a proper amount of caffeic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 3, 4-dicaffeoylquinic acid and 3, 5-dicaffeoylquinic acid, 4, 5-dicaffeoylquinic acid, forsythiaside D, baicalin, scutellarin, chrysin-7-O-glucuronide, oroxylin-7-O-glucuronide and a wogonin reference substance, and adding methanol to prepare a solution which is 20-50 [mu]g in 1ml. according to the phlegm-heat clearing injection fingerprint spectrum establishment method provided by the invention, the method is simple and convenient to operate, stable in result, high in reproducibility and high in precision.

The invention belongs to the field of genetic engineering, specifically to a production method for chlorogenic acid and dicaffeoylquinic acid by establishing a hairy root culture system through genetic transformation of stevia rebaudiana with agrobacterium rhizogenes. The method comprises the following steps: preparation of an explant of stevia rebaudiana, activation and culture of agrobacterium rhizogenes, suspension culture and production of chlorogenic acid and dicaffeoylquinic acid. PCR detection results show that hairy roots of stevia rebaudiana are generated through transformation; HPLC detection results show that the hairy roots of stevia rebaudiana can produce chlorogenic acid and dicaffeoylquinic acid. Since the hairy roots used in the invention have the characteristic of rapid growth on a hormone-free medium, the method provided by the invention has the advantages of simple operation, low cost, no restriction by natural conditions like climate and land, etc. A stable and sustainable novel drug source and food function factors are provided for production of chlorogenic acid and dicaffeoylquinic acid through culture of the hairy roots, and a reliable source and a reliable substance base are provided for large scale production of chlorogenic acid and dicaffeoylquinic acid by using a bioreactor in the future.

The invention discloses a thin-layer chromatography discriminating method of schizomussaenda dehiscens. According to the invention, schizomussaenda dehiscens is taken as a main component, a mixed solution of chlorogenic acid, 3,5-O-dicaffeoylquinic acid, and 4,5-O-dicaffeoylquinic acid is taken as a reference substance solution, and a thin-layer chromatography (Chinese pharmacopoeia 2015 edition general rule 0502) is referred for testing. The method has the advantages of convenient operation, simple equipment, easy color development, and fast expansion rate, and provides basis for discriminating the schizomussaenda dehiscens.

The invention relates to a thin-layer chromatography identification method for a wild chrysanthemum medicinal material and formula granules. The thin-layer chromatograms of the wild chrysanthemum medicinal material and formula granules are compared with the chromatograms of a wild chrysanthemum reference medicinal material as well as chlorogenic acid, linarin, 4,5-dicaffeoylquinic acid, 3, 5-O-dicaffeoylquinic acid, galuteolin and luteolin reference substance; so that effective components in the wild chrysanthemum medicinal material and formula granules can be rapidly and effectively identified. three isomers namely, chlorogenic acid, 4,5-dicaffeoylquinic acid and 3, 5-O-dicaffeoylquinic acid and two natural flavonoid compounds, namely,the galuteolin and luteolin in the wild chrysanthemumflower medicinal material and formula granules are identified by thin-layer chromatography for the first time; and the wild chrysanthemum flower medicinal material and the formula granules can be enriched, separated and purified at corresponding Rf positions of a silica gel plate by using the method. The chlorogenic acid, the isomeride component of chlorogenic acid, and the flavonoid compounds canbe prepared, so that high commercial value can be realized. The method is environmentally friendly and convenient to operate, good in repeatability and specificity and good in application prospect.

The invention relates to a construction method and a quality detection method of a UPLC feature map of a Hangzhou chrysanthemum medicinal material. The construction method comprises the following steps: sample solution preparation; taking the Hangzhou chrysanthemum medicinal material, crushing, adding a solvent for extraction and filtering, wherein a filtrate is taken as a sample solution; reference solution preparation: taking chlorogenic acid, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, galuteolin and linarin and adding a solvent for dissolution, wherein an obtained solution isa reference solution; ultra performance liquid chromatography detection: absorbing the sample solution and the reference solution, injecting into a liquid chromatograph and detecting to obtain the UPLC feature map of the Hangzhou chrysanthemum medicinal material. The UPLC feature map is rich in characteristic peak information, can fully reflect the chemical composition of the Hangzhou chrysanthemum medicinal material and can also effectively characterize the qualities of a Hangzhou chrysanthemum decoction piece and standard decoction and identify the product authenticity.

The invention relates to the technical field of effective component determination, and in particular relates to a method for simultaneously determining content of five chemical components in chrysanthemum medicinal materials. By adoption of the method provided by the invention, the content of chlorogenic acid, luteolin-7-O-beta-D-glucoside, luteolin-7-O-beta-D-glucuronide, 3, 5-dicaffeoylquinic acid and apigenin-7-O-beta-D-glucoside can be measured simultaneously. According to the method provided by the invention, the separation degree is high, the separation of all the components can achievethe baseline separation, the linear range is large, the sensitivity is high, and the repeatability is high; the sample addition recovery rate of the five chemical components ranges from 95% to 105% ina sample addition recovery test, which all meet the specification. Therefore, the method provided by the invention provides an effective detection means for the chrysanthemum medicinal materials withthe five chemical components as quality detection indexes, and the quality characteristics of the chrysanthemum medicinal materials can be comprehensively reflected.

The invention provides a method for storing honeysuckle flowers. The method comprises the following steps: removing water from buds or blossoms of newly collected largeflower-like honeysuckle by virtue of steam, and drying to obtain fresh honeysuckle; putting the fresh honeysuckle in vacuum package with vacuum degree of -0.01-0.1MPa, and storing in shade for 0-18 months in an environment with relative humidity of 10-30 percent and temperature of (-20)-5 DEG C. The honeysuckle stored by adopting method, and the quality of the medicinal material is more stable, wherein the color value of the medicinal material is slightly changed, and the contents of characteristic components, such as chlorogenic acid, caffeic acid, 3,5-dicaffeoylquinic acid, galuteolin, macranthoidin B, dipsacoside B and the like are slightly changed as well.

The invention belongs to the field of plant biotechnology engineering, and particularly relates to a method for improving flavonoid phenylpropanoid compounds of a saussurea involucrate cell culture. In a culture medium used in the method, the phosphorus element concentration is 0.5-3 mmol/L, the NO3-ion concentration is 20-35 mmol/L, the NH4+ ion concentration is 0-30 mmol/L, and the Ca 2+ ion concentration is 0.5-3 mmol/L; the Mg 2+ ion concentration is 0.2-1.5 mmol/L, and the concentration of boron ions is 0.02-0.1 mmol/L; inducers or precursors or inhibitors of bypass metabolism may be added to the culture medium. Finally, the total flavone content of the saussurea involucrate cell culture and the content of rutin, chlorogenic acid, Syringin and 1, 5-dicaffeoylquinic acid are obviouslyimproved.

A composition for prevents or slows the appearance of blemishes associated with inflammation of the skin and/or scalp, or eliminates such, the composition including, per 100% by mass: a) 60.0%-75.0% by mass of an organic solvent chosen from 1,2-propanediol, 1,3-propanediol, 1,4-butanediol, 1,3-butanediol, 1,2-butanediol, 2-methyl-2,4-pentanediol, 1,6-hexanediol, 1,8-octanediol, or a blend of the above compounds b) 0.1%-2.0% by mass of a composition including a mass quantity x₁, expressed as mass equivalent of 1-O-(2-caffeyol) maloyl-3,5-O-dicaffeoylquinic acid, greater than or equal to 200 mg/g of a compound of general formula (I):

in which Q₁-Q₅ independently of each other represent the hydroxyl radical or a salt thereof or a radical selected among the caffeoyl, maloyl, caffeoyl maloyl and maloyl caffeoyl radicals. At least one of Q₁-Q₅ represents neither the —OH radical nor a salt thereof; and c) 20.0%-35.0% by mass water.

The invention discloses a method for rapidly separating and purifying polyphenol compounds in sorbus pohuashanensis berries. The method comprises the steps as follows: mashing sorbus pohuashanensis berries, adding an ethanol solution for ultrasonic extraction, and performing concentration under reduced pressure to obtain crude extract of sorbus pohuashanensis berries; performing gradient elution by a polyamide chromatographic column with ethanol as an eluant, and performing concentration under reduced pressure to obtain eluates with different gradient fractions; separating and purifying the eluates with different gradient fractions with HSCCC (high-speed counter-current chromatography) to obtain neochlorogenic acid, chlorogenic acid, quercetin-3-O-(6''-alpha-L)-rhamnosyl-4'''-alpha-L-rhamnosyl)-beta-D-glucoside, 3,5-O-dicaffeoylquinic acid and rutin with purity higher than 95% respectively. The method adopts a simple process, is high in separation speed and low in comprehensive cost and has very good promotional and use values, and the product purity is high.

The invention relates to a method for preparing a plurality of caffeoylquinic acid monomers from honeysuckle, and belongs to preparation methods for chemical substances or drugs. In the prior art, caffeoylquinic acid substances prepared by the existing method do not have high purity; the existing chlorogenic acid extraction and preparation technology has a disadvantage of high organic solvent consumption so as to cause environmental pollution, or adopts a complex separation technology, such that production enlarging is difficult; and extraction efficiency is not high, raw material utilization rate is low, and other problems exist. With the present invention, the problems in the prior art are solved, and high purity 3-caffeoylquinic acid, high purity 4-caffeoylquinic acid, high purity 2-caffeoylquinic acid, high purity 4,5-dicaffeoylquinic acid, high purity 3,4-dicaffeoylquinic acid and other monomer components are obtained. In addition, the project technology comprises a raw material pretreatment step, an extraction step, a macromolecule precipitation step, a macroporous resin separation step, a reversed phase column separation step, a crystallization step and the like, such that production cost is saved, production efficiency is improved, the process is simple and easily enlarged into industrialization production, and the raw material utilization rate can be greatly improved.