48 results about "Dicaffeoylquinic acid" patented technology

The invention discloses a construction method and an identification method of characteristic chromatograms of a senecio scandens medicinal material as well as decoction pieces, standard decoction and formula granules thereof, and relates to the technical field of traditional Chinese medicine identification. The construction method of the specific chromatogram comprises the following steps: extracting a to-be-detected sample with methanol to obtain a test solution; the method comprises the following steps: dissolving 5-caffeoylquinic acid, chlorogenic acid, caffeic acid, 4-caffeoylquinic acid, rutin, hyperoside, isoquercitrin and 4, 5-dicaffeoylquinic acid with methanol to obtain a reference solution; and a liquid chromatograph is adopted for determination. A chromatographic column of the liquid chromatograph takes phenyl-methyl polysiloxane as a stationary phase, acetonitrile as a mobile phase A and 0.05 vol%-0.3 vol% formic acid aqueous solution as a mobile phase B for gradient elution. The information of the characteristic chromatogram constructed by the method is rich, and the quality of the senecio scandens medicinal material, decoction pieces, standard decoction and formula granules can be quickly and comprehensively reflected.

The invention relates to the technical field of traditional Chinese medicine detection, and particularly provides a construction method of specific chromatograms of common carpesium fruit and a preparation thereof. Comprising the following steps: by taking octadecylsilane chemically bonded silica as a filling agent and a mobile phase comprising methanol and an acid-containing aqueous solution, carrying out gradient elution, and screening through a large number of experiments to obtain a specific elution procedure, so as to obtain 13 or more common characteristic peaks, and realizing the purification of the chlorogenic acid, the caffeic acid, the chlorogenic acid, the cryptochlorogenic acid, the 1, 4-dicaffeoylquinic acid, the 1, 4-dicaffeoylquinic acid, the 1, 4-dicaffeoylquinic acid, the 1, 4-dicaffeoylquinic acid and the 1, 4-dicaffeoylquinic acid. The method can effectively separate characteristic peaks of 2, 5-dicaffeoylquinic acid, cosmosiin and carabrone, is good in peak shape, stable in base line, short in detection time, provides a basis for quality detection and control of the common carpesium fruit and the preparation thereof, can fully reflect the integrity and characteristics of the common carpesium fruit and the preparation thereof, is simple to operate, and is suitable for industrial production. The method is suitable for quality control of different preparation types of common carpesium fruit.

The present invention relates to: a composition for preventing, alleviating or treating dry eye syndrome or meibomian gland dysfunction; and the like, and was completed by identifying the excellent effects of treating dry eye syndrome with 1,5-O-dicaffeoylquinic acid and improving meibomian gland function. 1,5-O-dicaffeoylquinic acid, a key component of the present invention, reduces corneal epithelial loss, increases the amount of tear production, inhibits inflammation of the lacrimal and meibomian glands, increases the area of the meibomian gland, increases the survival rate of corneal epithelial cells in a hyperosmotic environment, and can inhibit cell death. Therefore, the composition according to the present invention can effectively treat dry eye syndrome caused by environmental stress or various diseases and improve meibomian gland function, and thus is expected to be used as a therapeutic agent for various eye diseases.

The invention provides an NK (Natural Killer) cell culture medium, which is prepared by adding the following components into a 1640 culture medium: 8 to 11 percent v / v fetal calf serum, 18 to 22 mg / mL of recombinant human transferrin, 40 to 60 mu M of 1, 3-dicaffeoylquinic acid, 25 to 35 mu M of aceglutamide, 900 to 1100 IU / mL of human recombinant IL-2, 45 to 55 IU / mL of human recombinant IL-15, 45 to 55 IU / mL of human recombinant IL-21, 50 to 200 U / mL of penicillin, 50 to 150 mu g / mL of streptomycin and 1 to 2 mg / mL of more than 95 percent of polyporus gossypii polysaccharide. The NK cell culture medium provided by the invention can effectively improve the proliferation speed of NK cells and the killing effect on various tumor cells.

A method for extracting 3,5-O-dicaffeoylquinic acid from Huangshan Gongju and its application in drugs for protecting against alcoholic liver damage. The 3,5-O-dicaffeoylquinic acid has the following structural formula:The present disclosure selects the medicinal and edible homology plant, Huangshan Gongju, as the research object. This plant is abundant in resources and the raw materials are easy to obtain. Specifically, the present disclosure uses 70% ethanol to extract Huangshan Gongju, uses the method of bioactivity-guided isolation, and uses a FLA-2025-10-120A preparative liquid chromatograph to isolate a phenolic compound from the active site of Gongju that resists alcoholic liver damage. The compound separation method is simple, quick, and easy to operate.

The present invention relates to an anti-glycosylation composition for improving pores, improving skin barrier, improving wrinkles and resisting glycosylation, containing an artichoke extract in which 1, 5-dicaffeoylquinic acid and chlorogenic acid are increased. According to the artichoke, the state that the content of 1, 5-dicaffeoylquinic acid and chlorogenic acid in the extract is increased through raw material pretreatment engineering is achieved, and the pore size reducing effect and the sebum adjusting effect of the extract are remarkably improved; in addition, various skin health improving effects, skin barrier improving effects and the like, which are obtained by anti-glycosylation effects, are more excellent than those of the conventional artichoke extract, thereby exhibiting suitability of a highly functional cosmetic composition which can improve sensitivity and complex skin.

The invention belongs to the technical field of quality detection of traditional Chinese medicinal materials, and particularly relates to a quantitative analysis method for effective components of common goldenrop medicinal materials. The method comprises the following steps: a, preparation of a reference substance solution: precisely weighing neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, 3, 5-O-dicaffeoylquinic acid, quercitrin and 4, 5-O-dicaffeoylquinic acid reference substances, and adding methanol to prepare a mixed reference substance solution; b, preparation of a test solution: crushing a common goldenrop herb, adding a methanol solution for extraction, and filtering to obtain the test solution; and c, determination: respectively and precisely sucking the reference substance solution and the test solution, injecting into an ultra-high performance liquid chromatograph, determining, and calculating the content by adopting an external standard method. The method can simultaneously detect and analyze the contents of seven chemical components in the common goldenrop herb, has good linearity, repeatability, stability and recovery rate, and can effectively control the quality of the common goldenrop herb.

The present invention provides a method for simultaneously determining the contents of 8 components in Xiongju Shangqing Tablets. The HPLC method is used in the present invention to determine neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 1,3-O-dicaffeoylquinic acid, luteoloside, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid and 1,5-O-dicaffeoylquinic acid. The HPLC method is adopted in the present invention to establish a method for simultaneously determining the contents of 8 components such as neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 1,3-O-dicaffeoylquinic acid, luteoloside, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid and 1,5-O-dicaffeoylquinic acid in Xiongju Shangqing Tablets. The method is simple and highly specific, providing a basis for the establishment of the quality standard of Xiongju Shangqing Tablets. The established method can play a role in process control to a certain extent and provide a reference for the quality control and quality evaluation of the product.

The invention discloses a quality control method and application of eyesight-improving and kidney-nourishing tablets, and relates to the technical field of medicines.The control method comprises the steps that firstly, a fingerprint spectrum of the eyesight-improving and kidney-nourishing tablets is established and used for quality detection of the eyesight-improving and kidney-nourishing tablets, and effective components of six medicinal materials in the eyesight-improving and kidney-nourishing tablets can be detected; meanwhile, the content of glucose, salidroside, chlorogenic acid, verbascoside, catalpol, specnuezhenide, rehmannia D, 3, 5-O-dicaffeoylquinic acid, 4, 5-O-dicaffeoylquinic acid, chrysophanol, luteolin, luteoloside, aurantio-obtusin and beta-ecdysterone in a sample can be quantitatively detected. Establishment of the fingerprint spectrum detection method of the eyesight-improving and kidney-nourishing tablets and determination of standard fingerprint spectrum information of the eyesight-improving and kidney-nourishing tablets have important significance on work in the aspects of product quality control, authenticity identification, origin traceability and the like.

The present invention provides a method for detecting the fingerprint of Qingwen Hufei Granules. The detection method generates a standard fingerprint and determines a total of 14 common chromatographic peaks. The fingerprint detection method established by the present invention is tested on 14 batches of Qingwen Hufei Granules, and the similarity value is ≥0.943. The present invention also establishes a method for determining the content of 7 index components, namely, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, forsythiaside A, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, and angloside. The methodological results show that the method has good precision, stability, and sample recovery rate, and can be used for the intrinsic quality detection and identification of the Qingwen Hufei Granules traditional Chinese medicine composition. It has good market prospects in industrial large-scale production applications.

The invention discloses a construction method of a patrinia characteristic spectrum and a reference spectrum, and a method for distinguishing patrinia and counterfeit products. The construction method of the specific chromatogram comprises the following steps: respectively sucking a reference substance solution and a test sample solution for ultra-high performance liquid chromatography analysis, and constructing the specific chromatogram of a sample to be detected, the specific chromatogram comprising at least 14 characteristic peaks, four characteristic peaks in the characteristic peaks respectively correspond to a protocatechuic acid reference substance peak, a rhodamine reference substance peak, a 3, 4-O-dicaffeoylquinic acid reference substance peak and a 4, 5-O-dicaffeoylquinic acid reference substance peak; and carrying out qualitative analysis on the to-be-detected sample through the obtained characteristic chromatogram, and simultaneously identifying the patrinia scabiosaefolia, the patrinia villosa juss and the counterfeit patrinia herb. The method is simple to operate, and methodology verifies that the reproducibility and stability are good, the detection cost is low, and the detection efficiency is high.

The present invention discloses a bicycloalkane compound, a synthesis method and an application thereof. Using aryl dicyanoethylene and allyl carbonate as reaction raw materials, a bicycloalkane compound is obtained under the action of an organophosphorus catalyst. The advantages of the present invention include: high reaction efficiency and high yield; the organophosphorus catalyst is cheap, easily available, stable and has no pungent odor; no strong acid or strong base, as well as additional oxidizing or reducing agents need to be added during the reaction, and the conditions are relatively mild; no transition metal is used as a catalyst during the reaction, which is economical and environmentally friendly; the reaction substrates are easy to prepare. The compound represented by formula (III) of the present invention has good inhibitory activities against Penicillium digitaum and Rhizoctonia solani, and among them, the effect of compound 11 is the best, reaching 91% and 83% respectively.

A composition that prevents or slows the appearance of blemishes associated with inflammation of the skin and / or scalp, includes per 100% by mass: a) 60.0%-75.0% by mass of an organic solvent chosen from 1,2-propanediol, 1,3-propanediol, 1,4-butanediol, 1,3-butanediol, 1,2-butanediol, 2-methyl-2,4-pentanediol, 1,6-hexanediol, 1,8-octanediol, or a blend of the above compounds; b) 0.1%-2.0% by mass of a composition including a mass quantity x1, expressed as mass equivalent of 1-O-(2-caffeyol)maloyl-3,5-O-dicaffeoylquinic acid, greater than or equal to 200 mg / g of a compound of general formula (I):in which Q1 and Q3-Q5 independently of each other represent the hydroxyl radical or a salt thereof or a radical selected among the caffeoyl, maloyl, caffeoyl maloyl and maloyl caffeoyl radicals; at least one of Q1 and Q3-Q5 represents neither the —OH radical nor a salt thereof; and c) 20.0%-35.0% by mass water.

The application belongs to the technical field of medical diagnosis, and particularly relates to application of Abiotrophia and related metabolites in preparation of a Kawasaki Disease (KD) diagnostic kit. The metabolites are 1,2-dieicosenoyl-sn-glycero-3-phosphocholine and 1,3-dicaffeoylquinic acid. By detecting the levels of the above markers in the skin samples of subjects, non-invasive auxiliary diagnosis of Kawasaki Disease is achieved, and the problem of poor compliance of existing blood tests is solved.

The embodiment of the invention provides a saussurea involucrata cell culture and application thereof. The saussurea involucrata cell culture comprises the following components: a) 7 wt%-20 wt% of a flavonoid compound; b) a phenylpropanoid derivative, the content of which is 2.1 wt% to 14.1 wt%; c) a carboxylic acid and a derivative thereof, wherein the carboxylic acid and the derivative thereof comprise dicaffeoylquinic acid in an amount of 1.6 wt% to 35 wt%; and d) other components.

The present invention relates to a NK cell culture medium and a NK cell culture method, relating to the field of cell culture technology, comprising a basal culture medium and additives, wherein the components and concentrations of the additives are: 200-400 IU / L of cytokines, 3-6 mmol / L of nutritional supplements, 1-2 mmol / L of metabolic regulators, 90-110 mg / L of vitamin C, 150-300 μg / mL of plant polysaccharides, 50-100 μmol / L of 1,3-dicaffeoylquinic acid, 48-52 μg / mL of nano-hydroxyapatite, 14-16 μmol / L of signaling pathway regulators, 5-10 IU / L of heparin sodium, and 10-80 μg / mL of astragaloside IV liposomes. The culture medium and culture method can efficiently expand NK cells and maintain their activity and functional stability.

Disclosed herein are pharmaceutical compositions and uses thereof in treating Parkinson's disease. The pharmaceutical composition comprises an ethanol extract of an herbal mixture consisting of Artemisia argyi, Morus alba L., Leonurus japonicus Houtt, Capsicum annuum L., Lophatherum gracile Brongn, Curcuma longa, and Glycyrrhiza uralensis; and a pharmaceutically acceptable excipient. The ethanol extract comprise 14 ingredients, including chlorogenic acid, leonurine, schaftoside, rutin, isochaftoside, isochlorogenic acid, 4,5-dicaffeoylquinic acid, quercetin, apigenin, glycyrrhizic acid, bisdemethoxycurcumin, demethoxycurcumin, curcumin and artemisetin.

This invention provides a method for extracting active ingredients from *Erigeron breviscapus*, belonging to the field of pharmaceutical technology. Based on the differences in the physicochemical properties of the compounds, this invention first achieves preliminary separation of caffeic acid esters and scutellarin, followed by further purification and separation based on the properties of the two types of substances. Specifically, this invention uses ethanol-water solutions of different concentrations to extract caffeic acid esters stepwise, and uses macroporous and microporous resins for preliminary separation of the caffeic acid esters. Scutellarin is then preliminarily separated through acid-base treatment. Finally, high-purity scutellarin, 3,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, and scutellarin are obtained through reversed-phase industrial chromatography. This invention achieves the extraction and separation of multiple active ingredients from *Erigeron breviscapus*, and all solvents used in the extraction and separation process are recyclable, resulting in low cost.

The present invention discloses a method for preparing Artemisia selengensis leaf microcapsules with XOD inhibitory activity. By extracting and purifying the Artemisia selengensis leaves, most impurities are removed and dicaffeoylquinic acid is enriched, which not only improves the formability of the drug but also enhances the uric acid-lowering efficacy. The present invention selects suitable wall materials and optimizes the process conditions for microcapsule preparation, so that the prepared microcapsules have the advantages of high drug embedding rate, high yield, good taste and stability, and the like.

The application discloses application of 1,5-dicaffeoylquinic acid in prevention and / or treatment of non-alcoholic fatty liver disease and weight loss and blood lipid reduction, and belongs to the technical field of biological medicine. The application first proposes application of 1,5-dicaffeoylquinic acid in preparation of a medicine for preventing and / or treating metabolic related fatty diseases. In-vivo and in-vitro experimental results show that after 1,5-dicaffeoylquinic acid is used in an in-vitro and in-vivo NAFLD model, the weight can be reduced, liver function and blood lipid abnormality, sugar metabolism abnormality and lipid accumulation can be relieved; the 1,5-dicaffeoylquinic acid can be used for preparing a medicine for preventing or treating NAFLD, obesity and blood lipid reduction, and the new use of the 1,5-dicaffeoylquinic acid in resisting NAFLD is confirmed, so that the 1,5-dicaffeoylquinic acid is expected to be developed into a new type of NAFLD resisting medicine, and has a wide market prospect.

Provided is a method for preventing, alleviating or treating dry eye syndrome or meibomian gland dysfunction that may be caused by environmental stress or various diseases, including administering 1,5-O-dicaffeoylquinic acid which reduces corneal epithelial loss, increases the amount of tear production, inhibits inflammation of the lacrimal and meibomian glands, increases the area of the meibomian gland, increases the survival rate of corneal epithelial cells in a hyperosmotic environment, and can inhibit cell death, and thus is expected to be used as a therapeutic agent for various eye diseases.

The invention provides a method for determining the content of a wolfberry-chrysanthemum-rehmannia concentrated pill, which adopts a UPLC (Ultra Performance Liquid Chromatography) multi-wavelength segmented integral method, realizes simultaneous determination of eight components, namely 5-hydroxymethylfurfural, morroniside, chlorogenic acid, loganin, paeoniflorin, 4, 5-O-dicaffeoylquinic acid, benzoyl paeoniflorin and paeonol for the first time, breaks through the limitation that only 2-3 components are detected by traditional HPLC (High Performance Liquid Chromatography), and has a wide application prospect. And the detection efficiency is improved by more than 50%. By optimizing the extraction solvent and time, the extraction rate of each component is greater than or equal to 95%, and the recovery rate is 97-102%. A gradient elution procedure of acetonitrile-methanol-0. 3% phosphoric acid is adopted, and components with large polarity difference are effectively separated. The integral wavelength is dynamically switched based on 3D spectral data, so that insufficient sensitivity or interference of a single wavelength is avoided, and the quantitation limit of low-content components is increased by 10 times. The method solves the problem of single detection component in the prior art, comprehensively covers the key active components of the four main medicines in the formula, remarkably improves the detection efficiency and accuracy, and provides a scientific basis for quality control and evaluation of the wolfberry-chrysanthemum-rehmannia concentrated pill.

The invention belongs to the field of medicine detection, and particularly relates to a method for detecting the content of effective components in Qingyin tea. The invention relates to a method for detecting the content of citroobtusin, chlorogenic acid, luteoloside, 3, 5-O-dicaffeoylquinic acid and 4, 5-O-dicaffeoylquinic acid, which comprises the following steps of: measuring the content of citroobtusin, chlorogenic acid, luteoloside, 3, 5-O-dicaffeoylquinic acid and 4, 5-O-dicaffeoylquinic acid by adopting a triple quadrupole multiple reaction monitoring (MRM) quantitative mode through an ultra-high And establishing a standard curve of the five effective components, and calculating the content of the effective components through the standard curve and the sample peak area. The method can effectively separate five effective components, can perform simultaneous quantitative analysis, is quick to operate, good in repeatability and accurate and reliable in result, and can provide a basis for comprehensive quality control of the Qingyin tea.

The present application belongs to the technical field of quality detection of traditional Chinese medicinal materials, and particularly relates to a method for quantitatively analyzing effective components of Xiaochaihu medicinal materials. The method comprises the following steps: a, preparation of a control solution: precisely take new chlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, 3,5-O-dicaffeoylquinic acid, quercitrin and 4,5-O-dicaffeoylquinic acid control samples, and add methanol to prepare a mixed control solution; b, preparation of a test solution: crush Xiaochaihu medicinal materials, add a methanol solution for extraction, and filter to obtain the test solution; and c, determination: precisely take the control solution and the test solution, inject them into an ultra-high performance liquid chromatograph, and determine the content by using an external standard method. The method can simultaneously detect and analyze the content of seven chemical components in Xiaochaihu medicinal materials, has good linearity, repeatability, stability and recovery rate, and can effectively control the quality of Xiaochaihu medicinal materials.

The present invention relates to an external preparation of an anthelmintic Vernonia extract and a preparation method thereof. The method uses the anthelmintic Vernonia extract as a raw material drug to prepare an external preparation of a gel and a microemulsion gel. The gel and microemulsion gel obtained by the method of the present invention have uniform appearance and stable physical and chemical properties, and the content of the main components, Vernonia oleracea and 3,5-dicaffeoylquinic acid, is stable as determined by HPLC; and the intradermal retention and cumulative transdermal amount of the main components of the external preparations such as the prepared gel and microemulsion gel are evaluated through a transdermal test, and it is determined that the microemulsion gel has a higher intradermal retention capacity and is very suitable for topical administration treatment. The present invention uses Vernonia oleracea and 3,5-O-dicaffeoylquinic acid as the main components of the preparation for the first time, and prepares an external preparation with uniform appearance and stable content.

The invention discloses a method for improving curcumin embedding capacity of myofibrillar protein, and belongs to the technical field of food processing. The method for improving curcumin embedding capacity of myofibrillar protein comprises the following steps: dissolving myofibrillar protein powder in a low-salt solution, and carrying out ultrasonication to obtain a protein solution; adding an enzyme into the protein solution, then reacting, and deactivating the enzyme to obtain a mixed solution; adjusting the pH value of the mixed solution to 11-13, adding 4, 5-dicaffeoylquinic acid into the mixed solution, and then reacting in a dark place to obtain the isochlorogenic acid C-ultrasonic-assisted enzyme treated myofibrillar protein compound. According to the method, ultrasonic-enzyme and 4, 5-DCQA treatment is combined, so that the structure of the myofibrillar protein is effectively improved, and the curcumin embedding capacity of the myofibrillar protein is remarkably improved.

The invention provides application of 1, 5-dicaffeoylquinic acid in preparation of drugs for improving and treating diseases related to mitochondrial dysfunction, and belongs to the technical field of medicine preparation. By reducing the active oxygen level in cartilage cells, improving the mitochondrial membrane potential, increasing the ATP content in the cartilage cells, promoting the mitochondrial structure recovery, up-regulating the expression level of NDUFA6 protein, up-regulating the expression level of MYL 3 protein and regulating the relative expression level of p-AMPK / AMPK protein, the invention improves and treats the mitochondrial dysfunction, and can be used for treating the chondrial dysfunction. The compound can be applied to preparation of drugs for improving and treating mitochondrial dysfunction.

The present application relates to the technical field of traditional Chinese medicine detection, and specifically provides a characteristic spectrum construction method of Herba Centipedae and its preparation. A specific elution procedure is obtained through a large number of experiments by using octadecylsilane bonded silica gel as a filler and a mobile phase including methanol and an acid-containing aqueous solution for gradient elution, 13 or more common characteristic peaks are obtained, and effective separation of characteristic peaks including neochlorogenic acid, caffeic acid, chlorogenic acid, cryptochlorogenic acid, 1,4-dicaffeoylquinic acid, 1,5-dicaffeoylquinic acid, big pothos glycoside and thymotin lactone ketone is achieved, the peak shape is good, the baseline is smooth, the determined components can be completely separated, the detection time is short, and the method provides a basis for quality detection and control of Herba Centipedae and its preparation, can fully reflect the integrity and characteristics of Herba Centipedae and its preparation, and is simple to operate and applicable to quality control of different preparation types of Herba Centipedae.

The invention discloses a traditional Chinese medicine composition for preventing and treating cerebral arterial thrombosis and a screening method thereof, and the effective components of the traditional Chinese medicine composition comprise the following substances in parts by weight: 30-50 parts of 3-caffeoylquinic acid, 20-40 parts of 4, 5-dicaffeoylquinic acid and 30-50 parts of scutellarin. The traditional Chinese medicine composition disclosed by the invention has an obvious improvement effect on cerebral arterial thrombosis, and can also protect damaged neurons, improve the activity of nerve cells and effectively relieve cerebral arterial thrombosis injury. Meanwhile, according to the screening method of the composition, a regression model with brain protection related indexes as dependent variables and the proportion of all the components as independent variables is established through mixed material design, the predictive ability of the model is evaluated, and after the predictive ability of the model reaches the standard, synchronous optimization of the multiple brain protection indexes is carried out; and solving the optimal ratio of each component and carrying out experimental verification. The traditional Chinese medicine composition screened by the method is stable in activity and exact in effect.

The invention discloses a process for preparing plant residue mask paper and a mask product thereof, and relates to the technical field of cosmetics. The method comprises the following steps: treating olive residues by using a 1-vinyl-3-methylimidazolium chloride modified solution, and generating polyimidazolium salt macromolecules at high temperature; polyimidazole salt macromolecules can form a film to wrap squalene and other active ingredients in the olive residues, oxidative degradation can be slowed down, the slow release effect of the mask sheet can be remarkably prolonged, and meanwhile the film forming performance can improve the fitting degree and moisture retention of the mask sheet. In addition, a quaternary ammonium structure of polyimidazole salt macromolecules has an inhibiting effect on staphylococcus aureus and the like, and the antibacterial performance of the mask sheet can be improved. Secondly, the olive fruits are used as the main raw material to prepare the essence, and 4, 5-O-dicaffeoylquinic acid methyl ester is added, so that free radicals are neutralized to a great extent, and the anti-oxidation effect of the essence is remarkably improved.