The invention discloses an efficient collybia radicata culture medium prepared from highland barley straw. The efficient collybia radicata culture medium comprises, by weight, 50-60 parts of highland barley straw, 30-40 parts of barley straw, 20-30 parts of sunflower stems, 2-3 parts of oxalic acid, 1-2 parts of cellulose, 20-30 parts of potato flour, 15-25 parts of corn flour, 22-32 parts of bran, 5-8 parts of ant powder, 0.6-0.8 part of bacillus subtilis, 0.8-0.1 part of candida utilis, 1.1-1.3parts of saccharomycetes, 1-2 parts of polyacrylic acid sodium, 2-3 parts of sepiolite powder, 5-7 parts of turf, 0.7-0.9 part of potassium dihydrogen phosphate, 0.4-0.6 part of calcium sulfate, 0.5-0.6 part of magnesium sulfate, 0.1-0.2 part of vitamin C, 3-4 parts of brown sugar, 3-4 parts of quick lime, 0.15-0.2 part of potassium sorbate, 1-2 parts of attapulgite and a proper amount of water. The efficient collybia radicata culture medium is rich in nutrient, and collybia radicata can be efficiently cultivated.