77 results about "Pear tree" patented technology

The invention relates to the technical field of microorganisms, and particularly discloses a saline-alkaline tolerant bacillus deserticola BW-13 with biocontrol and growth promoting functions and application thereof. The bacillus safensis BW-13 is preserved in the China Center for Type Culture Collection on January 13, 2025, the classification name of the bacillus safensis BW-13 is bacillus safeensis, and the preservation number of the bacillus safensis BW-13 is CCTCC NO: M 2025108. The strain BW-13 has relatively high salt tolerance, and still keeps good resistance under various stress conditions; meanwhile, the plant growth promoting agent has various plant growth promoting characteristics including nitrogen fixation, phosphorus solubilization and cellulase and protease secretion capacity, and pot experiment verifies that the plant growth promoting agent can improve the salt resistance of cucumber seedlings and promote plant growth; the strain has an antagonistic effect on a plurality of phytopathogens, and especially has a remarkable prevention and treatment effect on plant diseases such as apple ring spot, polygonatum kingianum anthracnose, pear tree rot and tomato gray mold caused by ascomycophyta pathogenic fungi.

The invention relates to the technical field of microorganisms, in particular to a strain of Senotrophomonas sepilia DB3 with disease-resistant, iron-dissolving and growth-promoting functions and application thereof, and the strain is separated from calcareous soil pear tree rhizosphere and has good capabilities of producing IAA (Indoleacetic Acid), quickly growing, dissolving insoluble iron, producing iron carriers and effectively antagonizing various pathogenic bacteria. Pot experiments show that the DB3 microbial inoculum can significantly promote growth of peanut and pyrus betulaefolia seedlings, improve root development and iron nutrient content, enhance leaf SPAD value and iron absorption and relieve iron-deficiency yellows, can be used for preparing biological bacterial fertilizers and reducing chemical iron fertilizer investment, and has good development and application prospects.

The invention provides a method for regulating and controlling phenological periods of outdoor pear trees, and belongs to the technical field of pear tree cultivation. According to the phenological period delaying method suitable for the outdoor cultivation pear orchard in the south, the phenological period of pears can be delayed by 5-7 days through grass growing in winter, grass covering in early spring and irrigation in early spring, soil temperature rising is restrained, root activity recovery is restrained, and the phenological period of pears can be delayed by 5-7 days. Specifically, by maintaining high soil water content and low soil temperature, inhibiting respiration and physiological activity recovery speed of root systems of pear trees in spring and delaying absorption and transfer of moisture and nutrients by the root systems, the metabolism level of the tree bodies is reduced, and the phenological period of the pear trees is delayed by about 7 days. Through the synergistic effect of vegetation covering and moisture regulation, accurate delay of the phenological period of the pear tree is achieved, operation is easy and convenient, the ecological cultivation requirement is met, and the method is suitable for open-air pear cultivation areas in the south.

The invention provides an orchard building method and a pruning method of a double-layer dwarf open-center-shaped high-photosynthetic-efficiency cultivation pear orchard, and belongs to the technical field of fruit tree cultivation. According to the orchard building method for the high-photosynthetic-efficiency cultivation pear orchard, multiple factors such as the sun drift angle, the day photosynthetic efficiency change around pear trees, the geographic latitude of the orchard and the growth period of pear varieties are comprehensively considered, and the planting row direction and the high-photosynthetic-efficiency tree form are determined and pruned according to the latitude of the place where the orchard is located and the day photosynthetic efficiency change around the pear trees. By means of the orchard building method, by selecting a high-light-effect tree form and a scientific pruning method, controlling the height and size of a tree crown, thinning and removing over-dense branches on the upper portion and adjusting a fruiting branch group, the ventilation and light transmission conditions in the tree crown can be effectively improved, shielding of the tree crown to lower leaves is reduced, and illumination of the lower leaves is further improved. The method is simple and easy to implement, convenient to popularize and apply, and capable of remarkably improving the pear tree photosynthesis efficiency and improving the fruit yield and quality of pears.

The invention belongs to the technical field of microorganisms, and discloses bacillus velezensis and application thereof in plant disease control. A bacillus velezensis strain is separated and screened from rhizosphere soil of a wolfberry plant in a Nomuliflood farm in Qinghai province, the strain is bacillus velezensis G3 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No. 36136. The bacterial strain has a better antibacterial effect on pathogenic bacteria fusarium oxysporum of Chinese wolfberry root rot, also has an inhibiting effect on pathogenic bacteria of Chinese wolfberry verticillium wilt, botrytis cinerea, sclerotinia rot of colza, alternaria alternata, apple anthracnose, apple rot, pear wilt and wheat scab, and has broad-spectrum antibacterial activity; wide application prospects are realized in plant disease prevention and treatment.

The application discloses application of a fungal virus in prevention and treatment of pear tree and apple tree Valsa diseases. The fungal virus is a positive single-stranded RNA virus, and a cDNA nucleotide sequence of the fungal virus is shown as SEQ ID No. 1. A fungus containing the virus is Valsa pyri HJL1-22 (gamma), the fungus is infected by the fungal virus of claim 1, the fungus is named as HJL1-22 (gamma), is classified and named as Valsa pyri, and has a preservation number of CGMCC No. 41449 in China General Microbiological Culture Collection Center. The virus Vphv1-gamma can be secreted to outside of cells, therefore, culture liquid filtered off mycelium can be used as a biocontrol agent for prevention and treatment of pear tree and apple tree Valsa diseases, and can effectively weaken or inhibit pathogenicity and diffusion of Valsa pyri and Valsa mali, and has a prospect for being used for producing and practicing biological prevention and treatment of pear tree and apple tree Valsa diseases.

This invention relates to the field of fruit tree pollination equipment, specifically to an integrated device for precise pollination of pear tree inflorescences and constant temperature maintenance of pollen activity. The device includes a guide tube, with a constant temperature tube sleeved outside the guide tube and fixedly connected to it. One end of the guide tube has a gas path switching component for switching gas flow direction. The gas path switching component includes a switching cavity fixedly connected to one end of the guide tube, with several air inlets on the sidewall of the switching cavity. The end of the switching cavity away from the guide tube is fixedly connected to the inner sidewall of the constant temperature tube, and the end of the constant temperature tube away from the switching cavity has several exhaust holes. A T-shaped slider is slidably fitted onto the inner sidewall of the switching cavity. This invention, through the gas path switching component and the telescopic guide hood assembly, solves the problems of poor pollen activity maintenance due to large temperature fluctuations before spraying and during transport in existing pear tree inflorescence pollination processes, as well as inaccurate pollination caused by external wind disturbances during pollination.

The utility model relates to the field of tree pruning devices, in particular to a dwarf close planting pear tree pruning device which comprises a power rod and a supporting cylinder, a shearing blade buffering mechanism is arranged between a fixing frame and the supporting cylinder, and the buffering mechanism comprises a moving block connected to the inner wall of the supporting cylinder in a sliding mode. Two triggering blocks are fixedly connected to the top end of the moving block, and two cylinder bodies are fixedly connected to the inner top wall of the supporting barrel; when a moving rod is arranged to drive a shearing blade to cut thick branches, the moving rod drives a trigger block on a moving block to compress gas in a cylinder body through a piston, impact of the blade on a fixing frame is reduced, device damage is avoided, and therefore the purpose of prolonging the service life of the device is achieved; and a rotating sleeve is arranged on the cylinder body to be matched with rotating connection of a positioning block and a moving rod, so that the device can be rotated and adjusted to a position most suitable for self power generation, and the use convenience of workers is improved.

The invention relates to the technical field of genetic breeding, and particularly provides a gene related to drought tolerance of pear trees and application of the gene, the gene is specifically a PbAREB3 gene, the nucleotide sequence of the gene is shown as SEQ ID NO.1, and the amino acid sequence of the gene is shown as SEQ ID NO.2; the PbAREB3 gene provided by the invention can be used for effectively judging the drought tolerance of various pear tree materials, and the PbAREB3 gene with high expression quantity is high in corresponding drought tolerance; according to the gene, the screening efficiency can be effectively improved, and meanwhile, a guidance thought can be effectively provided for cultivating a drought-resistant pear tree transgenic material.

The invention discloses a pear tree planting and seedling raising liquid supply treatment system, which comprises a supply box and uniformly distributed universal wheels, the universal wheels are installed at the bottom of the supply box, the inner wall of the supply box is fixedly connected with a partition plate, two liquid pumps are fixedly installed on the inner bottom wall of the supply box, and the two liquid pumps are fixedly connected with the partition plate. And the top end of the infusion pump is fixedly connected with a spraying pipe. According to the system, a large number of pictures and information in the pear tree planting and seedling growing process are stored in the storage module, when liquid is supplied to the pear tree seedling growing container, the industrial camera located in the front portion can conduct image collection on the state of pear seedlings, and therefore the growth state of the pear seedlings is judged through the comparison and analysis module, and the corresponding flow is set; when the nutrient solution is sprayed, monitoring and timely blocking are carried out through the flow sensing valve, so that automatic and accurate nutrient solution spraying can be realized, the steps and the labor amount required by personnel are reduced, and help can be provided for the personnel who do not understand the knowledge of pear tree planting and seedling raising.

This invention relates to a reverse spraying structure for treating diseases on the underside of pear leaves. It includes a mounting chassis, a first medicine tank, a second medicine tank, a spraying moving mechanism, a spraying adjustment mechanism, a spraying connection module, and a spraying module. The spraying moving mechanism drives the spraying adjustment mechanism to move linearly along a moving guide rail, achieving precise adjustment of the spraying position. The adjustment motor in the spraying adjustment mechanism drives the spraying pipe to rotate axially via a gear ring, causing the fan-shaped nozzle to spray the pesticide solution upwards from below the plant, directly targeting and infecting diseases such as black spot disease on the underside of pear leaves. The spraying connection module is equipped with a separating sealing plate. This invention divides the inner cavity of the spraying pipe into two independent chambers, each connected to one of the two medicine tanks. Combined with the limiting action of the intercepting bracket and the rotation of the spraying pipe to close the spraying nozzle, the pressure of the pesticide solution pushes the separating sealing plate to slide, achieving rapid switching between the two pesticide solutions with zero cross-contamination.

The application discloses application of a Pyrus betulaefolia E3 ubiquitin ligase gene PbrATL18 in genetic improvement of plant drought resistance and anthracnose resistance and belongs to the technical field of genetic engineering. The nucleotide sequence of the PbrATL18 gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 2. The plant drought resistance and / or anthracnose resistance performance is improved by overexpressing the PbrATL18 gene in the plant or improving the protein activity. The application constructs a gene overexpression Arabidopsis transformation vector and a gene silencing P. betulaefolia seedling transformation vector respectively, obtains positive plant seedlings, and carries out drought and anthracnose treatment. The results show that overexpression of the E3 ubiquitin ligase gene PbrATL18 can effectively maintain the balance of active oxygen in cells, keep the cell osmotic potential stable, and significantly improve the plant drought resistance and anthracnose resistance performance, thereby providing a new idea for high drought tolerance and disease resistance breeding of pear trees.

The utility model relates to the technical field of fertilization for dwarfing and dense planting of pear trees, in particular to water and fertilizer regulation and control equipment for dwarfing and dense planting of pear trees, which comprises a proportioning tank, and one side of the top of the proportioning tank is fixedly connected with a placement box; the regulation and control mechanism capable of precisely regulating and controlling water and fertilizer for dwarfing and close planting of the pear trees is arranged at the top end of the proportioning tank; by arranging a regulation and control mechanism, the upper end and the lower end of a packing auger in the middle of a placement box communicate with a placement groove and a weighing box correspondingly, the packing auger can convey solid water and fertilizer raw materials in the placement groove into the weighing box, water and fertilizer needing to be proportioned are regulated and controlled through a weighing disc, and a sealing plate can seal and intercept a groove in the bottom end of the weighing box; water and fertilizer leakage can be prevented during liquid water and fertilizer proportioning, the water storage barrel can adjust the position of the piston plate through the threaded rod, the position of the piston plate can be adjusted through the dial gauge, the pear tree dwarf close planting water and fertilizer can be accurately regulated and controlled, and the irrigation effect of the water and fertilizer is improved.

The present application belongs to the technical field of fruit tree cultivation, and particularly relates to a continuous trunk improved pear tree shape and a cultivation method thereof. Specifically, for the mature pear tree with open heart-shaped training, the vertical branch in the middle of the inner cavity of the tree body is selected as the stock after being short-cut and grafted with pollination branches to be the central trunk; the following year, the central trunk is short-cut in the middle in winter; in the third year, the central trunk plays the role of pollination in the flowering period and the inflorescences are removed after flowering; the main branches on the top of the central trunk are pulled to form a "tree on tree" shape; in the fourth year, the central trunk plays the role of pollination in the flowering period and the inflorescences are removed after flowering or the fruits are left after flowering, and the strong branches are removed; in the fourth winter, the main branches of the central trunk are removed to leave the weak and curved branches, and the side branches are removed to leave the strong and vigorous branches. The method can reduce the germination rate of the vertical overgrowth branches on the main branches of the original tree by more than 40%, significantly saving the nutrients of the tree body, increasing the average fruit setting rate of the inflorescences, and significantly improving the single fruit weight of the fruits due to the pollen collective effect, and increasing the orchard output value.

The invention provides a composition for preventing and treating rot of pear trees as well as a preparation method and application of the composition, and belongs to the technical field of pesticide preparation. The composition is prepared from osthole and tebuconazole. The biological source bactericide cnidium lactone and the chemical bactericide tebuconazole are combined for preventing and treating the bergamot pear tree rot disease or inhibiting the bergamot pear tree rot disease, the effect of preventing and treating the bergamot pear tree rot disease is improved, and the synergistic interaction effect is achieved. The method for preparing the composition is simple and easy to implement. According to the composition, the use of a chemical agent tebuconazole is reduced, a way for improving the environment and reducing pollution is provided, and a basis is provided for sustainable and long-term development of a method for preventing and treating the rot diseases of the bergamot pear trees.

The utility model relates to the technical field of supporting frames, in particular to a foldable pear tree branch supporting frame which is characterized in that an H-shaped angle adjusting seat is rotatably connected to the interior of a telescopic reinforcing rod, a supporting ring is fixedly connected to the outer surface of the telescopic reinforcing rod, and a jacking spring is fixedly connected to the interior of the supporting ring. And the H-shaped angle adjusting seat rotationally connected in the telescopic reinforcing rod is manually screwed, so that the H-shaped angle adjusting seat rotates around a connecting point. During rotation, the inclined surface of the arc-shaped clamping groove in the H-shaped angle adjusting seat extrudes the triangular limiting block, so that the triangular limiting block compresses the jacking spring to slide down. When the H-shaped angle adjusting seat rotates to the position of the next arc-shaped clamping groove, the triangular limiting block loses extrusion force, the triangular limiting block is ejected and clamped into a new clamping groove under the elastic force effect of the jacking spring, due to the five arc-shaped clamping grooves, limiting of the adjusting angle of the H-shaped angle adjusting seat is achieved, the H-shaped angle adjusting seat is stably kept at the corresponding screwing installation position, and the placement position is stably kept. And a worker can conveniently finish screwing installation work with one hand.

The utility model provides a kind of multi-band trapping device for pear tree disease and insect pest control, it is related to pest control device technical field, including base, the top of base is fixedly installed with mounting seat, the top of mounting seat is provided with thread structure, in the utility model, on the one hand, by setting rotating assembly, by half gear, gear drives rotating shaft rotation, and then make connecting pipe and install above connecting frame and stick insect board rotate.Stick insect board of rotation can capture pest from multiple angles, avoid the capture blind area that fixed type trapping device can exist, improve the efficiency of trapping pest, on the other hand, by the thread structure of base top mounting seat and the thread structure of net cover bottom cooperation, so that net cover can be conveniently installed and disassembled, simultaneously, stick insect board is connected by magnetic attraction strip and connecting frame, when the pest captured on stick insect board is more and needs to be replaced, staff can easily take down stick insect board from connecting frame.

The utility model discloses a pear tree insect-proof net cover convenient to mount and dismount, which relates to the technical field of pear tree insect prevention and comprises a cover body, two zippers are arranged on the cover body, a chain ring is arranged below the cover body, a connecting pipe is arranged at the connecting end of the chain ring, two pressing blocks are movably sleeved with the connecting pipe, and a pear tree is completely covered by the cover body. A chain ring penetrates through a group of connecting holes formed in the lower end of the cover body and is tightened at the moment, so that the lower end of the cover body is tightly attached to the pear tree trunk, the chain ring is fixedly connected with the two clamping blocks, and the whole cover body is tightly attached to the trunk, so that pests can be prevented from entering from a gap between the cover body and the trunk; the pears are protected, damage of pests to the pears is reduced, growth and fruit quality of the pears are protected, the cover body can be better fixed to the pears through fixing, displacement and damage caused by wind blowing shaking or tree trunk friction are avoided, the service life of the cover body is prolonged, and continuous and stable protection is provided for the pears.

A growth model construction based evaluation method for branch pulling of trellised pear trees. A "double-armed parallel" trellised pear tree is taken as a research object, branch pulling treatment is carried out on one-year shoots of the pear tree, a linear or nonlinear regression equation is established by detecting relevant indexes of the length and thickness of the shoots and the growth of fruits, and the dynamic trend of fruit quality formation under different treatment conditions is analyzed. Results show that, in a trellised cultivation mode, different branch treatment angles are closely related to the transport efficiency of carbon from a "source" (branches and leaves) to a "sink" (fruits) of and the growth vigor of the pear tree. The nutritional management of the branches and the fruits can be coordinated and enhanced within 95-110 days after full blooming, so that the formation of the fruit quality and the stabilization of the tree vigor in the later period can both be ensured. The solution provides a theoretical basis for formulating rational technical measures for standardized and refined management of "Cuiguan" trellised pear tree bodies.

The utility model discloses water and fertilizer integrated precise irrigation equipment for pear trees, which comprises a water pipe, a water source interface, a seal head, support blocks, a three-way pipe, a connecting pipe, a joint and a tree-surrounding irrigation structure, and is characterized in that the water source interface is mounted at the rear end of the water pipe, the seal head is plugged at the front end of the water pipe, and the support blocks are distributed at the bottom of the side end of the water pipe; the three-way pipe is distributed at the side end of the water pipe, the bottom of the connecting pipe is connected to the three-way pipe, the connector is installed at the top end of the connecting pipe, and the tree-surrounding irrigation structure is connected to the connecting pipe through the connector. The utility model solves the problems that the existing water and fertilizer integrated irrigation equipment mostly performs drip irrigation or slow spraying on one side of a pear tree, so that the irrigation is inaccurate, the root development is unbalanced, the water and fertilizer absorption is uneven, and the growth is influenced.

The invention discloses a soil digging device convenient for planting small pear saplings, which comprises a trolley, the outer side of the trolley is provided with a traction device, the inner wall of the trolley is fixedly connected with a triangular block, the triangular block is slidably connected with a soil sampling plate, the left side of the soil sampling plate is downwards inclined, the triangular block is fixedly connected with a first electric push rod, and the first electric push rod is fixedly connected with a second electric push rod. The left end of the first electric push rod is fixedly connected with the soil sampling plate. According to the soil loosening trolley, soil in an area needing soil loosening and stones in the area can be shoveled up and screened together, the stones and caked soil can be screened out, then the caked soil can be smashed and then discharged, only the stones are left in the trolley, the existing mode that blades are used for digging soil is replaced, and the soil loosening effect is effectively improved; and moreover, stones in the soil can be screened out, so that the influence of the stones on the subsequent planting and growth of small pear trees or other crops is effectively avoided.

The application discloses a method for accelerating and early fruiting of pear hybrid branches, a cultivation device and a method, and steps are as follows: grafting a top bud of a second-year pear hybrid branch to a perennial pear rootstock, realizing growth of the top bud into a fruiting branch, and realizing provision of fruiting nutrition by the pear rootstock, so that a technical problem that the pear hybrid branch needs to be fruiting for at least 5-10 years is solved, and thus the pear hybrid fruiting branch is obtained in the third year.

The application discloses a method for improving the calyx abscission rate of Korla fragrant pear, which comprises the following steps: after the flowering period, a DDC treatment agent is used to treat the inflorescence part of the Korla fragrant pear tree so as to improve the calyx abscission rate of the Korla fragrant pear. The inflorescence part of the Korla fragrant pear tree is treated by using the DDC treatment agent. The DDC inhibits the SOD activity of the calyx, reduces the contact of the active oxygen with the SOD and other enzymes, increases the active oxygen in the calyx of the Korla fragrant pear, increases the lignin accumulation, promotes the lignification degree of the abscission layer cell wall to be increased, and thus the calyx abscission of the Korla fragrant pear is promoted, the calyx abscission rate reaches 69.50%, the calyx abscission rate is improved, and the fruit quality of the Korla fragrant pear is improved. Compared with the conventional calyx abscission agent, the DDC is safe and reliable, and the Korla fragrant pear tree will not be damaged by the DDC in the whole year, so that the long-term and healthy development of the Korla fragrant pear industry is facilitated.

The invention discloses a compound bactericide for a pear tree pollination period and application thereof, and belongs to the technical field of fruit tree planting and pesticide application. According to the method, 35% propiconazole and carbendazim compound suspoemulsion (7% propiconazole and 28% carbendazim) serves as a core agent, after being diluted by 2200 times, the suspoemulsion is sprayed in the full-bloom stage of the pear trees in the mode of avoiding the bee collection peak period, and bee colony protection measures are matched. By optimizing the concentration and spraying time of the compound bactericide and matching management, the stress effect of the bactericide on Italian bees is remarkably reduced on the basis of ensuring that the calyx abscission rate of pear fruits is increased (male pears are changed into female pears) and the fruit quality is improved, the death rate of the bees within 24 hours is reduced, and the survival rate of the bees is increased. The enrichment amount of the compound bactericide in bee bodies and bee pollen is controlled, and the bee pollination efficiency is not influenced. The method gives consideration to production benefits and ecological safety of pear trees, and is simple to operate, high in practicability and suitable for application of the compound bactericide in the flowering phase of a large-scale pear tree plantation.

The invention discloses a cultivation method for improving resistance and quality of northern pear trees through stock-scion coordinated regulation and control, and belongs to the technical field of fruit tree cultivation. The cultivation method specifically comprises the following steps: (1) rootstock cultivation; (2) stock treatment; (3) scion treatment; (4) combination of rootstocks and scions; (5) performing low-temperature dynamic stress training; (6) spectrum monitoring; and (7) spraying the mixed solution. The invention discloses a cultivation method for improving cold resistance, drought resistance and fruit quality of northern pear trees through stock-scion coordinated regulation, through three core links of innovating stock-scion combination, implementing dynamic stress training and precise fertilizer and water regulation, the limitation of a traditional technology is overcome, the cold resistance and drought resistance of the northern pear trees are improved, and the yield of the northern pear trees is improved. Meanwhile, the fruit quality is improved.

This invention relates to a remote sensing extraction method for pear orchard areas based on the Re-UNet model, which overcomes the shortcomings of inaccurate classification results and low efficiency in pear orchard area extraction from remote sensing images compared with existing technologies. The invention includes the following steps: acquiring a remote sensing image dataset; constructing the Re-UNet pear orchard area extraction model; training the Re-UNet pear orchard area extraction model; acquiring and preprocessing the remote sensing images of the pear orchard areas to be segmented; and obtaining the remote sensing extraction results for the pear orchard areas. Based on the UNet semantic segmentation model, this invention solves the overfitting problem that easily occurs in small datasets. It also incorporates spatial and channel attention mechanisms and a residual module, further enhancing the feature transfer and cumulative integration characteristics of pear orchard areas in high-resolution remote sensing images, effectively reducing the "salt and pepper" phenomenon and misclassification, and improving the overall segmentation accuracy.

The application belongs to the technical field of microorganisms and particularly relates to a strain of DB3 with disease-resistant iron-dissolving and growth-promoting functions Stenotrophomonas sepilia The strain DB3 is isolated from the rhizosphere of pear trees in calcareous soil, has good abilities of IAA production, rapid growth, dissolution of insoluble iron, iron carrier production and antagonism to various pathogenic bacteria. Through potting tests, it is shown that the DB3 bacterial agent can significantly promote the growth of peanut and pear seedlings, improve the root development and iron nutrient content, enhance the SPAD value and iron absorption of leaves, and relieve the iron deficiency chlorosis, and can be used for preparing a biological bacterial fertilizer, reducing the input of chemical iron fertilizer, and has a good development and application prospect.

The application discloses a method for identifying leaf chlorophyll SPAD value of pear germplasm, which comprises the following steps: A, investigating time step; B, investigating method step; C, determining leaf chlorophyll SPAD value of pear germplasm step; D, dividing leaf chlorophyll SPAD value identification grading standard step of pear germplasm; E, determining and grading leaf chlorophyll SPAD value of pear germplasm to be determined. The application has the following advantages and effects: after the steps of determining the investigation time and the investigation method, the determination of the leaf chlorophyll SPAD value of the pear germplasm, according to the normal distribution diagram of the measured leaf chlorophyll SPAD value of the pear germplasm, the leaf chlorophyll content identification grading standard of the pear germplasm is formulated, and the leaf chlorophyll SPAD value of the pear germplasm to be determined is determined and graded. Finally, the leaf chlorophyll content grading standard of the pear germplasm is formulated, the leaf chlorophyll content grade of the pear germplasm resource can be quickly determined through the measured leaf chlorophyll SPAD value, a scientific basis is provided for pear excellent germplasm resource excavation and pear tree production management.

The invention belongs to the technical field of agricultural cultivation, and particularly relates to a method for improving disease resistance by regulating and controlling pear garden soil microflora based on non-woven fabric coverage. According to the method, the pear garden soil is covered with polylactic acid-based biodegradable non-woven fabric, and the soil microbial structure is adjusted in cooperation with a compound microbial agent containing bacillus or actinomycetes. The polylactic acid-based non-woven fabric has good heat insulation and water retention effects, can stabilize the temperature and humidity of soil, can be naturally degraded, and has no environmental pollution risk; after degradation, proliferation of beneficial antagonistic bacteria can be promoted, and growth of pathogenic microorganisms is inhibited. The compound microbial agent can further inhibit the growth of pathogenic microorganisms and enhance the disease resistance of pear trees. The problem of micro-plastic pollution of a traditional plastic mulching film can be effectively solved, and the requirements of green agriculture and sustainable development are met.