91 results about "Xylem" patented technology

The invention discloses application of a PdeTLP protein and a related biological material thereof in regulation and control of plant root system development. Experiments prove that compared with wild poplar, the number of adventitious roots, the number of adventitious root lateral roots, the root length and the root included angle of PdeTLP knockout poplar are obviously increased, and the plant height, the number of stem nodes and the stem diameter are also obviously increased. In addition, the xylem width, the phloem width and the xylem cell area of the poplar with the PdeTLP knocked out are obviously higher than those of the wild poplar. The PdeTLP gene participates in regulation and control of the root system number, the root system length, the root system included angle, the plant height, the stem node number, the stem diameter and the secondary microtubule tissue development of the poplar. The method not only has important significance on molecular mechanism and theoretical research of regulating and controlling the number and length of poplar root systems, plant height, number of stem nodes and stem diameter, but also has important guiding significance on directionally cultivating trees meeting actual production requirements.

The invention relates to the field of agricultural bioengineering, in particular to application of an oilseed rape BnaA08G0167600WE gene in enhancing pod shattering resistance of oilseed rape. According to the invention, after a transgenic plant is constructed in oilseed rape by utilizing a modern biotechnological means and the oilseed rape gene BnaA08G0167600WE is knocked out, the silique of the mutant is not easy to crack compared with a wild type under the action of external force, and a separation layer and a wood layer of the cross section of the silique of the mutant have more cell connections compared with the wild type, so that the yield of the mutant is improved. And the generation of the mutants provides an important genetic material for further researching the function of the gene and the effect of the gene in the rape.

The invention relates to a PagNRT3.1 gene for regulating xylem development of poplar and application of the PagNRT3.1 gene, and belongs to the technical field of plant genetic engineering and biology. The nucleotide sequence of the PagNRT3.1 gene for regulating and controlling the xylem development of the poplar is shown as a sequence 1 in a sequence table; the amino acid sequence of the expression protein of the PagNRT3.1 gene is shown as the sequence 2 in the sequence table. The PagNRT3.1 gene is transferred into poplar 84K, and compared with a wild type, the transgenic poplar over-expressing the PagNRT3.1 has a phenotype with increased xylem width, which indicates that the PagNRT3.1 gene is the key for regulating and controlling the development of the xylem of the poplar and has important application value in the field of high-yield genetic engineering of forest trees.

The invention discloses a mulberry twig clamping and positioning method and system, belongs to the technical field of intelligent mulberry leaf picking equipment, and aims to solve the technical problems that an existing RGB camera is easily interfered by illumination, and picking damage is caused due to the fact that a demarcation point of a tender branch and an old branch is difficult to accurately recognize. According to the method and system, 500-600 nm visible light and 750-900 nm near-infrared band images are synchronously collected through a multispectral imaging device, HSV color space parameters are extracted, and a color gradient model is established; by analyzing the gradient change rate of the hue value H and the saturation degree S and combining the curvature change rate and the near-infrared reflectivity difference value, the transition boundary of the tender and old branches is accurately judged, and the three-dimensional coordinates of the clamping datum point are generated by dynamically shifting 5-15 mm towards the side of the tender branches. According to the scheme, the limitation of single hue detection is broken through, the xylem recognition capability is enhanced by utilizing multispectral information, the clamping accuracy is improved, the method is suitable for automatic operation of a top-remaining picking mode in large-scale silkworm and mulberry planting, the picking efficiency can be effectively improved, and branch damage can be reduced.

This invention discloses a key miRNA that regulates peony plant height, stem diameter, and xylem formation, and its applications. The precursor and mature sequences of this peony miRNA are shown in SEQ ID NO.1 and SEQ ID NO.2, respectively. The peony miRN82 provided by this invention can be used to regulate peony growth. Overexpression of miRN82 reduces peony plant height and stem diameter, and narrows the xylem width, indicating that miRN82 is a key miRNA regulating peony plant height, stem diameter, and xylem formation. This invention regulates peony plant height, stem diameter, and xylem thickness by overexpressing or knocking out / silencing miRN82, enabling the breeding of peony varieties with different stem heights, thicknesses, and hardnesses according to specific needs. Therefore, it has significant application value in peony molecular breeding.

The invention relates to a PagNLP8 / 9a gene for regulating xylem development of poplar and application of the PagNLP8 / 9a gene, and belongs to the technical field of plant genetic engineering and biology. The nucleotide sequence of the PagNLP8 / 9a gene for regulating and controlling the development of the xylem of the poplar is shown as a sequence 1 in a sequence table; the amino acid sequence of the expression protein of the PagNLP8 / 9a gene is as shown in the sequence 2 in the sequence table. The PagNLP8 / 9a gene is transferred into poplar 84K, and compared with a wild type, the transgenic poplar over-expressing the PagNLP8 / 9a has a phenotype with increased xylem width, which indicates that the PagNLP8 / 9a gene is the key for regulating the development of the xylem of the poplar and has important application value in the field of high-yield genetic engineering of forest trees.

The application discloses a kind of based on the growth environment multi-factor comprehensive monitoring method and system of old tree protection, including step (S1) non-invasive perception network construction: in the crown layer area of old tree deployment meteorological monitoring unit, real-time acquisition photosynthetic active radiation intensity PAR and crown layer surface temperature T c ; with trunk as center, soil sensor array is radially laid in root distribution area, and soil volume water content θ and soil water potential are monitored in layers;Micro-invasive thermal diffusion probe is installed at the breast diameter of trunk, and xylem original liquid flow flux SF raw Is continuously measured, and initial liquid flow flux SF raw Calibration is carried out;Foundation interferometric radar is erected outside the protection zone, and three-dimensional spatial distribution point cloud P r Of root is obtained by surface scanning.This application can realize more comprehensive old tree monitoring, and timely find the existing abnormality of old tree environment.

The invention provides a spore solid bacterium inoculation method. Relates to the technical field of edible mushrooms. The method comprises the following steps: S1, culture medium group preparation: selecting trees and setting a spore source culture medium group and a receptor culture medium group in groups; s2, inducing the spore source culture medium group to form a mature sporocarp, inoculating a target edible mushroom strain on the spore source culture medium group, inducing the strain to grow and forming the mature sporocarp; s3, controlling environmental parameters to promote spores to eject and fall on the receptor culture medium group; s4, the sporocarp is obtained through natural culture, the spore source culture medium group accounts for 5%-20% of the total culture medium group in volume, the surface of the receptor culture medium group in the S1 needs to be subjected to scribing treatment, the depth of a notch is 2-5 mm, the condition that the notch makes contact with the xylem is adopted as the criterion, and if the length of the tilia wood is smaller than 30 cm, scribing is not needed. The spore solid bacterium inoculation method provided by the invention has the advantages of reducing the planting cost and improving the economic benefit.

The utility model provides a shearing sampling device for preventing plants from generating aeroembolism, belongs to the technical field of plant sample sampling, and solves the technical problems that air enters a xylem conduit to form the aeroembolism at the moment of shearing in the prior art, a moisture transport channel is blocked, and leaves are dehydrated, withered and even dried off and fall off. A shear sampling device capable of preventing plants from generating aeroembolism comprises a drive-by-wire chassis, a sampling frame fixed on the drive-by-wire chassis, a supporting rod fixed on the sampling frame, a regulation and control rod connected to the supporting rod in a sliding mode, an electric hydraulic clamp fixed to the regulation and control rod, a soaking cavity formed in the sampling frame, and a net cage arranged in the soaking cavity, a first regulation and control assembly for controlling the height of the net cage is arranged in the sampling frame, a second regulation and control assembly for controlling the height of the regulation and control rod is arranged in the supporting rod, and a driving assembly for controlling the shearing head to conduct shearing action is arranged in the regulation and control rod. The plant branch cutting device has the advantages that plant branches can be cut in water, and aeroembolism is prevented.

The invention relates to the technical field of facility horticulture and plant nutrition regulation and control, and discloses a soil conditioning synergistic selenium-rich tomato cultivation method which comprises the following steps: applying nano mineral powder and a biological nano selenium nutrient solution after tomato transplanting and seedling recovering; the method comprises the following steps: carrying out physical conditioning on soil water content in a first fruit expanding period of tomatoes so as to construct a physiological premise of xylem flow blocking, and spraying a biological nano-selenium nutrient solution on leaf surfaces in the state; performing topping treatment in a time window from 12 hours to 24 hours by taking a spraying completion moment as a starting point, and physically removing a growing point at the top end of the plant; according to the method, through the synergistic effect of soil rhizosphere environment conditioning and plant physiological intervention, under the condition of not depending on a chemical chelating agent, the problem that the selenium element is transported to leaves in a one-way mode is solved, and the selenium element can be directly transported to the leaves; the efficient targeted pumping and enrichment of the selenium element from the leaves to the fruits are realized.

The water production system described herein uses subsurface temperatures to cool water to 55 degrees Fahrenheit. That geothermally cooled water is then sent down the interior of a copper column. Thermal transfer then causes the surface of the copper column to cool. In warm humid environments, condensation will form on the outside of the copper column, much like water condenses on a glass of ice water. Alternative formats include adding a pressure sleeve to the copper column, changing the column into a sphere, and for the pressure-sleeved version dropping the entire thing down a hole wherein the outer surface of the pressure sleeve comes into contact with subsurface ground temperatures cooling the pressure sleeve and creating additional cooling surface area. Alternative formats also include placing the invention inside of telephone or light poles and using artificial xylem and evapotranspiration to draw geothermally cooled water up the column.

The invention discloses a healing promoting agent for melon vegetable grafted seedlings. The healing promoting agent contains abscisic acid and ribulose phosphate. The effect of promoting communication of phloem and xylem of grafted seedlings can be achieved by spraying the healing promoting agent before grafting, the healing time of the melon vegetable grafted seedlings is shortened, the operation of film uncovering and film covering in the healing period is reduced, the healing period management is simplified, and the healing cost is reduced.

The present disclosure provides a method for evaluating a seedling plant character of a transgenic poplar and use of an overexpressed pagEXPA1 gene in promoting xylem development of the poplar, relating to the technical field of forest wood character determination. The method for evaluating the seedling plant character of the transgenic poplar in the present disclosure includes the following steps: S1. detecting whether or not a foreign gene is integrated into a poplar genome through PCR; S2. detecting the expression level of the foreign gene in the poplar; S3. conducting a microstructural analysis on stem vascular tissues of the transgenic poplar; and S4. separating fibers, ducts, and sieve-tube cells through fiber isolation, and staining the isolated fibers to observe the morphology and structure of the fibers, ducts, and sieve-tube cells.

The application discloses a solid-phase extraction agent and a preparation method and application thereof. Raw materials of the solid-phase extraction agent include cigar stems and shavings, and the mass ratio of the cigar stems to the shavings is 1:1-2. The above raw materials are all natural plants, and in addition to being rich in vascular systems and cellulose, the structure formed by xylem and phloem arranged in bundles and a variety of capillary pore structures make the pores rich, and the structure is provided with rich polar hydroxyl and hydrophobic aromatic functional groups. After being prepared as the solid-phase extraction agent, the structure can form pi-pi bonds and hydrogen bonds with benzo[a]pyrene, has strong adsorption capacity for the benzo[a]pyrene and high adsorption stability, and due to the rich pores, has good permeability. The speed of the benzo[a]pyrene in the solution after adsorption through the extraction column is faster, the adsorption enrichment and purification efficiency of the benzo[a]pyrene in the solution after adsorption is high, and the cost is low and the structure is green and environment-friendly.

This invention discloses a vascular bundle-targeted bio-selenium nanoparticle, its preparation method, and its application, belonging to the field of plant treatment technology. The invention involves mixing and dissolving bio-selenium nanoparticles, water, 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, and N-hydroxysuccinimide, followed by activation to obtain an activated bio-selenium nanoparticle solution. A xylem-anchored peptide and MES buffer are then mixed and dissolved to obtain a xylem-anchored peptide solution. The activated bio-selenium nanoparticle solution and the xylem-anchored peptide solution are mixed, and the pH is adjusted to 6.3-6.8 before stirring to obtain the vascular bundle-targeted bio-selenium nanoparticle. The vascular bundle-targeted bio-selenium nanoparticle provided by this invention achieves interception of heavy metal transport pathways by specifically anchoring to xylem vessels. Compared with existing technologies that rely solely on broad-spectrum physiological antagonism, its efficiency in reducing heavy metal content in crop stems and leaves represents a qualitative leap.

The application discloses a kind of karst region crop precision irrigation methods based on stem sap flow early warning, comprising: step 1, layout underground infiltration irrigation system drip irrigation pipeline and surface water retaining layer;Step 2, install heat pulse sap flow sensor on healthy planting pearl stem;Step 3, according to the early warning signal generated by heat pulse sap flow sensor sap flow rate, as the starting signal of infiltration irrigation system;Step 4, after starting infiltration irrigation system, start underground infiltration irrigation system to carry out targeted water replenishment, when the monitored real-time average sap flow rate falls to normal level, close irrigation valve, complete closed-loop irrigation;Realize the combination of karst mountainous area drought early warning and root closed-loop targeted irrigation, effectively avoid the death of plant due to catastrophic hydraulic failure in xylem, significantly improve water resource utilization efficiency and crop drought survival rate;Solve the technical problems such as irrigation timing lag and low water resource utilization efficiency in karst region.

The application relates to the technical field of detection, in particular to a preparation method of sapwood section of Picea smithiana wood; the method comprises the following steps: fixing the sapwood sample of Picea smithiana after trimming in a fixing solution, softening the fixed sapwood sample of Picea smithiana in an ethylenediamine solution for 5-6 days, slicing the sapwood sample of Picea smithiana after the softening is completed, bleaching, washing and dyeing and eluting the sapwood section of Picea smithiana, making the section of the sapwood sample of Picea smithiana transparent by using an ethanol and xylene mixed solution, and sealing and shooting the section of the sapwood sample of Picea smithiana; the application increases the contact area of the sample with the FAA solution and the ethylenediamine solution, accelerates the fixing and softening speed of the sample, shortens the softening time from one month to 5-6 days, and realizes the rapid and efficient preparation of the sapwood section of Picea smithiana wood.

The present invention relates to a method for rapidly and efficiently extracting a plant essential oil from a plant raw material, particularly from xylem of a pine tree, thereby maximizing the yield, reducing the manufacturing cost and time, improving the productivity, performing high-purity treatment on the extracted plant essential oil by a refining process, thereby realizing the diversification and commercialization of the extracted oil, and improving the quality of the plant essential oil. The invention relates to a plant essential oil extraction method and plant essential oil extracted oil extracted by the extraction method.

The application discloses a method for preventing and treating trunk pear fire blight spots of fragrant pear, which comprises the following steps: a medicament mixed solution is prepared by mixing bacillus amyloliquefaciens diluted 300-400 times with water and manganese zinc oxide diluted 400-500 times with water in a weight ratio of 1-1.5:1-1.5; when the trunk of the fragrant pear is diseased in June every year, diseased tree bark at a trunk pus flow is cut off by using an electric saw without damaging the trunk wood; the prepared medicament mixed solution is evenly applied to the disease spots by using a brush, and the second application is performed after one week. The method is simple, fast, low in cost and high in operability, the prevention effect can reach 100% when the trunk disease spots have not caused the trunk to appear the dead symptoms, and the prevention effect can reach about 75% when the trunk has appeared the dead symptoms, the trunk can gradually recover, new buds can be generated, and new branches can be formed.

The invention belongs to the technical field of planting, and particularly relates to a method and application for promoting cutting rooting of cubilose fruits, and the method comprises the following steps: obliquely cutting the base of a stem section of the cubilose fruits to remove meat, and exposing xylem by 3-5cm to obtain cuttings; airing the cuttings for more than 7 days, performing cuttage, and applying a composite rooting agent to obtain cutting seedlings; the composite rooting agent comprises the following effective components: a nitrogen-potassium compound fertilizer, humic acid, a seaweed extract and chitosan. According to the method disclosed by the invention, the base part of the stem section is obliquely cut to form an inclined cut, so that the contact surface of the callus is increased, and more adventitious roots are promoted to germinate; meanwhile, cutting is conducted after the cuttings are aired for 7 days or above, a composite rooting agent is applied, rooting of the cuttings can be promoted, the rooting rate is remarkably increased, the grown roots are long, thick and strong, and the survival rate of cutting seedlings is also remarkably increased.

This invention relates to the field of biotechnology, specifically disclosing a method for improving the biocompatibility of poplar trees. PagBEH3b Methods for promoting poplar lignite development and / or increasing poplar height and / or increasing poplar yield through gene expression. This invention involves... PagBEH3b Genes were transferred into poplar trees and overexpressed. PagBEH3b Compared with wild-type, transgenic poplar showed a significant increase in xylem, indicating that the xylem of the brassinolide signaling pathway is a key regulator of this pathway. PagBEH3b It is a key regulatory factor in controlling xylem development in poplar. This invention provides a new option for regulating xylem development and has significant application value in the field of forest tree genetic engineering.

The application discloses a kind of saline-alkali soil crops stem xylem water potential prediction method and related equipment.The method comprises: using cloud migration optimization algorithm to optimize nonlinear Arp algorithm parameters, and cloud migration optimization algorithm and nonlinear Arp algorithm are constructed into integral cloud migration optimization-nonlinear Arp algorithm;Based on the stem xylem water potential data set collected, the crop stem xylem water potential model constructed by cloud migration optimization-nonlinear Arp algorithm is trained, and SHAP interpreter is used to optimize input factor;Based on the trained crop stem xylem water potential model, the saline-alkali soil crop stem xylem water potential is predicted.It can solve the problem that the traditional soil moisture or soil water potential determination method can only determine soil matric potential, it is difficult to determine the superposition water potential of osmotic potential and matric potential, and it is more difficult to accurately determine the time when crop physiological drought occurs.

The invention provides a device for repelling longhorn beetle larvae in a trunk by using a high-voltage electric shock principle, which comprises a power supply connected with a high-voltage generator through a connecting wire; a frequency adjusting knob is mounted on the high-voltage generator, the high-voltage generator is respectively connected with the electrode probe and the loop electrode through connecting wires, a control switch is mounted on the connecting wire of the high-voltage generator and the electrode probe, and the electrode probe and the loop electrode are inserted into a tree trunk during use. High-voltage electric shock directly acts on insect channels in trunks, current penetrates through xylem, accurate electric shock killing of longhorn beetle larvae is achieved, the defects that chemical pesticides are difficult to permeate the trunks and do not act directly are overcome, the prevention and treatment efficiency is remarkably improved, and the prevention and treatment cost is reduced. And the problems of insufficient ultrasonic penetrating power, inapplicable power grid structure and the like in the prior art are solved.

The invention discloses a method for accurately and efficiently evaluating nitrogen absorption and soil nitrogen supply conditions by measuring the nitrate content of bleeding sap of corn stalks. The method comprises the following steps: 1) culturing corn seedlings to grow to V8-V10 periods; 2) collecting stem bleeding sap in the morning of the second day after the plant is fully irrigated: transversely cutting the vertical stem in the second internode by using a blade until the stem is completely cut off; after standing for 5-60 minutes, bleeding sap seeps from the xylem from the cross section of the stalk, and after 5 minutes, the bleeding sap is condensed into liquid drops; using a sterile syringe to suck bleeding sap and transfer the bleeding sap into a centrifugal tube; after the bleeding sap is collected once and 3-10 minutes later, the bleeding sap is condensed into liquid drops again; collecting for multiple times to obtain bleeding sap meeting NO3 <-> determination requirements; 3, the NO3 <-> content of the stem bleeding sap is determined.According to the method, used consumables are simple and easy to obtain, the operation process is simplified, the method is easy to master, time is saved, and the method is especially suitable for obtaining nitrogen absorption phenotypes of large groups of corn in the field.

The invention relates to the technical field of tree seedling grafting, in particular to an automatic auxiliary grafting device for fruit tree seedlings. The device comprises a positioning cover matched with the shape of the outer wall of a sapling, and the protruding side of the positioning cover is communicated with a working cavity; a transverse nicking tool is arranged on the upper portion in the working cavity, slides in the transverse direction and is used for nicking a transverse opening in the skin of a sapling, and a separating tool is arranged on the side, close to the positioning cover, of the transverse nicking tool and slides in the longitudinal direction; the longitudinal cutter is arranged in the middle of the separating cutter, so that a longitudinal opening cut by the longitudinal cutter is certainly located in the middle below a transverse opening, and the situation that due to experience difference of grafting personnel, the position of the cut longitudinal opening deviates is avoided. The epidermis cut in the longitudinal direction is picked up by the separating knife moving synchronously with the longitudinal cutting knife, and compared with a traditional grafting knife that the epidermis on the two sides of a longitudinal opening needs to be picked up through the grafting knife after the longitudinal opening is formed in the epidermis. Not only is the operation procedure of grafting saved, but also the nicking tool is prevented from scratching the xylem of the sapling.

The invention discloses an integrated cultivation management system and method for codonopsis pilosula nnannf, and belongs to the technical field of cultivation management, and the method specifically comprises the following steps: collecting leaf reflection spectrum data and measuring internode length and leaf thickness in the early stage of row closing, calculating a saponin accumulation potential index, and dividing a land parcel into a high potential area and a low potential area according to the index; a phenolic acid substance rhizosphere is covered with a green light filtering light conversion film, and a low-potential area is covered with a light conversion film for increasing the proportion of an easily mineralized nitrogen source and enhancing red light transmission; monitoring the water potential of leaves in a high-potential area in a root system expansion period and implementing progressive moisture control, and determining an optimal harvesting period by monitoring a root system chromatic value and xylem hardness before harvesting; according to the method, a complete technical system from early diagnosis to differentiated management is constructed, and collaborative improvement of the quality and the yield of the codonopsis pilosula nnannf is achieved.

The invention relates to an application of a cotinus coggygria xylem extract in preparation of a neuroprotective drug. The traditional cognitive limitation of clearing heat and promoting diuresis of cotinus coggygria is broken through for the first time, the cotinus coggygria xylem extract with neuroprotective activity is disclosed, the extract can remarkably improve the survival rate of injured cells and shows broad-spectrum and remarkable neuroprotective activity, the preparation process is simple, reproducibility is good, large-scale production is easy, and the cotinus coggygria xylem extract is suitable for industrial production. A novel candidate substance and a preparation method easy to industrialize are provided for development of drugs for treating neurodegenerative diseases, and the compound has a good industrial transformation prospect.

The invention belongs to the technical field of plant paraffin sections, and discloses a preparation method of a paraffin section for analyzing the characteristics of xylem of a masson pine stem. The method comprises the following steps: (1) softening; (2) segmenting; (3) dehydrating; (4) transparentizing; (5) waxing; (6) embedding; (7) slicing, spreading, adhering and baking; (8) dewaxing and rehydrating; (9) dyeing and decolorizing; and (10) sheet sealing. According to the method, the preparation steps of the paraffin section are improved, specifically, the method comprises the steps of softener selection, wood part segmentation, dyeing time adjustment and the like, by adopting the technical scheme, the paraffin section with complete masson pine stem tissue and clear structure can be obtained, and the anatomical characteristics of the masson pine stem xylem are easy to observe and measure.

The invention relates to an agilawood forming agent and an agilawood forming method and application. It is found for the first time that a mixture of a fungus solution of appropriate wood-rotting fungi and non-wood-rotting fungi and an agilawood extract aqueous solution is adopted for inducing and treating agilawood (such as aquilaria sinensis) or quasi-aquilaria plants under appropriate conditions, agilawood without a rotting phenomenon can be obtained, and the agilawood production rate is high; meanwhile, it is found for the first time that under the appropriate condition that a fungus solution of wood-rotting fungi and non-wood-rotting fungi and an agilawood extract aqueous solution are subjected to mixed treatment, the non-wood-rotting fungi can generate volatile components with bacteriostatic activity, and the method has important significance in the aspects of preventing xylem tissue from rotting, improving stress resistance of trees and the like; therefore, the invention provides theoretical and practical scientific basis for innovation and optimization of the existing agilawood production technology, and has important significance for industrial production of agilawood.

The invention discloses a saline-alkali soil crop stem xylem water potential prediction method and related equipment. The method comprises the following steps: optimizing parameters of a nonlinear Arp algorithm by using a cloud migration optimization algorithm, and constructing the cloud migration optimization algorithm and the nonlinear Arp algorithm into an overall cloud migration optimization-nonlinear Arp algorithm; training a crop stem xylem water potential model constructed by a cloud migration optimization-nonlinear Arp algorithm based on the collected stem xylem water potential data set, and optimizing an input factor by using an SHAP interpreter; and performing saline-alkali soil crop stem xylem water potential prediction based on the trained crop stem xylem water potential model. The problems that a traditional soil moisture or soil water potential measuring method can only measure the soil matrix potential, it is difficult to measure the superimposed water potential of the osmotic potential and the matrix potential, and it is more difficult to accurately measure the physiological drought occurrence time of crops can be solved.