192 results about "Xylem" patented technology

The invention provides application of an ECT2 gene in regulating and controlling development of secondary xylem of a poplar. Transgenic poplar with overexpressed ECT2 genes is obtained through genetic transformation, it is found that overexpressed ECT2 genes can increase the width of secondary xylem of the poplar, and the nucleotide sequence of the ECT2 genes is shown as SEQ ID NO: 1. The ECT2 gene has a regulation effect on the development of the secondary xylem of the poplar.

The invention discloses application of a knockout PtoABAPT2 gene in increasing poplar biomass, and belongs to the technical field of plant genetic engineering. It is found for the first time that PtoABAPT2 can regulate and control the plant height, stem diameter, xylem cell layer number and cell wall thickness of the poplar, the PtoABAPT2 is edited through the CRISPR / Cas9 technology, a transgenic plant with the plant height, the stem diameter, the xylem cell number and the cell wall thickness remarkably increased is obtained, the total biomass of the transgenic plant is remarkably increased, and the transgenic plant has the advantages of being high in yield, high in yield and good in application prospect. Therefore, the wood yield and quality are improved, the wood production age limit is shortened, the production cost is reduced, and the wood utilization rate is improved. The invention provides a new gene resource for forest tree gene engineering, lays a foundation for wood genetic improvement and directed molecular breeding of forest trees, and has a wide application prospect.

The invention discloses a method for improving the character of a poplar, which comprises the following steps: highly expressing NAC73 gene in the poplar through a genetic engineering means; poplar character improvement includes: increasing poplar height; the number of poplar internodes is increased; the internode length of the poplar is increased; the poplar leaf area is increased; the xylem width of poplar is increased; and the poplar xylem catheter aperture is improved. The fast-growing poplar is cultivated, and a foundation is laid for cultivating fast-growing high-quality and high-yield new forest species through molecular breeding.

The invention discloses a high-survival-rate grafting method for grafting pinus sylvestris to pinus koraiensis in arid regions, which comprises the following steps: selecting pinus sylvestris stock with the growth period of 4-6 years, flatly cutting below a terminal bud branch point to form a cross section, downwards and obliquely cutting twice from the cross section to form a triangular pyramid-shaped chimeric structure, cutting a semi-lignified pinus koraiensis scion with a terminal bud, and grafting the semi-lignified pinus koraiensis scion with the terminal bud to obtain the pinus sylvestris scion. After main buds are reserved and lateral buds are cut off, two oblique triangular xylem sections are obliquely cut out from the lower portion of spores through two cutters, and a double-slope wedge-shaped joint body is formed; when the rootstock and the scion are cut, the cutting blade is immersed in an aqueous solution containing a plant growth regulator and a bactericide; the double-slope wedge-shaped joint body is inserted into the triangular pyramid-shaped embedding structure, so that the scion medullary layer and the stock medullary layer are overlapped; wrapping the interface with a composite hydrogel film containing a temperature-sensitive polymer and an antibacterial component; shading treatment is carried out after grafting is completed. The method can significantly improve the survival rate of grafted seedlings.

The invention discloses a method for creating cadmium-resistant rice with low grain cadmium accumulation and application. The rice oshma3oslct1 double mutant expressing the Cd binding autophagy adapter protein AIM-SpMTL-GFP is constructed through CRISPR-Cas9, so that the transfer of Cd from xylem to phloem and the accumulation of Cd in root vacuoles are reduced, meanwhile, the Cd binding autophagy adapter protein AIM-SpMTL-GFP can also accumulate cadmium in protoplast vacuoles of leaves, the yield and quality of rice grains are not influenced, and the yield and quality of the rice grains are greatly improved. Cd accumulation of grains is reduced, and Cd is enriched into straws at non-edible parts, so that industrial treatment is facilitated. The cadmium-resistant rice with low grain cadmium accumulation can reduce cadmium pollution in a food chain, so that the harm of cadmium to a human body is reduced.

The invention discloses application of a PdeTLP protein and a related biological material thereof in regulation and control of plant root system development. Experiments prove that compared with wild poplar, the number of adventitious roots, the number of adventitious root lateral roots, the root length and the root included angle of PdeTLP knockout poplar are obviously increased, and the plant height, the number of stem nodes and the stem diameter are also obviously increased. In addition, the xylem width, the phloem width and the xylem cell area of the poplar with the PdeTLP knocked out are obviously higher than those of the wild poplar. The PdeTLP gene participates in regulation and control of the root system number, the root system length, the root system included angle, the plant height, the stem node number, the stem diameter and the secondary microtubule tissue development of the poplar. The method not only has important significance on molecular mechanism and theoretical research of regulating and controlling the number and length of poplar root systems, plant height, number of stem nodes and stem diameter, but also has important guiding significance on directionally cultivating trees meeting actual production requirements.

The invention relates to the field of agricultural bioengineering, in particular to application of an oilseed rape BnaA08G0167600WE gene in enhancing pod shattering resistance of oilseed rape. According to the invention, after a transgenic plant is constructed in oilseed rape by utilizing a modern biotechnological means and the oilseed rape gene BnaA08G0167600WE is knocked out, the silique of the mutant is not easy to crack compared with a wild type under the action of external force, and a separation layer and a wood layer of the cross section of the silique of the mutant have more cell connections compared with the wild type, so that the yield of the mutant is improved. And the generation of the mutants provides an important genetic material for further researching the function of the gene and the effect of the gene in the rape.

The utility model relates to the technical field of forest fruit tools, and provides a fruit tree girdling device which comprises two girdling components, each girdling component comprises a girdling piece, a connecting piece and a handle piece which are connected in sequence, and each girdling piece comprises a double-layer girdling knife and a depth limiting piece arranged on the double-layer girdling knife; the connecting pieces of the two girdling components are movably connected, the knife edge parts of the double-layer girdling knives of the two girdling components are oppositely arranged, and the depth limiting piece is used for limiting the girdling depth of the knife edge parts. According to the fruit tree girdling device provided by the embodiment of the utility model, girdling is carried out on the fruit tree through the girdling pieces of the two girdling parts, girdling can be completed only by rotating each girdling piece for half a circle, the girdling efficiency is improved compared with the mode that girdling bypasses one circle, the girdling depth of the knife edge part is limited through the depth limiting piece, and damage of the knife edge part to the xylem of the fruit tree is avoided.

The invention belongs to the technical field of molecular biology, and particularly relates to a gene for regulating tree xylem development and application thereof. The nucleotide sequence of the gene for regulating and controlling tree xylem development is shown as SEQ ID NO. 1. By comparing the transgenic poplar overexpressing the MYB46 with a wild type, the phenotype that the xylem width of the transgenic poplar overexpressing the MYB46 is increased is found, so that the MYB46 gene is a key gene for regulating and controlling the development of the xylem of the poplar, and has important application value in the field of forest high-yield genetic engineering.

The invention relates to a PagNRT3.1 gene for regulating xylem development of poplar and application of the PagNRT3.1 gene, and belongs to the technical field of plant genetic engineering and biology. The nucleotide sequence of the PagNRT3.1 gene for regulating and controlling the xylem development of the poplar is shown as a sequence 1 in a sequence table; the amino acid sequence of the expression protein of the PagNRT3.1 gene is shown as the sequence 2 in the sequence table. The PagNRT3.1 gene is transferred into poplar 84K, and compared with a wild type, the transgenic poplar over-expressing the PagNRT3.1 has a phenotype with increased xylem width, which indicates that the PagNRT3.1 gene is the key for regulating and controlling the development of the xylem of the poplar and has important application value in the field of high-yield genetic engineering of forest trees.

The invention discloses an application and a method of a PtoKNATa gene in regulation and control of xylem development and wood formation of poplar, a knockout vector of the PtoKNATa gene is constructed and screened, and after the obtained knockout vector is transformed into a populus tomentosa plant, the xylem cell generation speed of the populus tomentosa can be effectively increased, the fast growth of wood is improved, and the yield of the poplar is increased. A method and a germplasm basis are provided for breeding of excellent varieties of forest trees, and the wood yield and economic benefits of economic forests can be improved.

The invention relates to the field of measurement and calibration of trunk sap flow, in particular to a calibration device and method for measuring trunk sap flow based on a heat dissipation method. A stable water potential difference is formed between a water tank in the calibration device and a water containing tray under the control of a Markov bottle, and water in the closed water containing tray is pressed into a trunk section, is gathered at the top of the trunk section and enters a volumetric flask through a water passing opening; the lower end of the trunk section is hermetically connected with the water containing tray, and the section of the top end of the trunk section is inclined and is connected with the acrylic cover plate; after the test is finished, weighing the weight of water passing through the trunk section in unit time, calculating the average liquid flux density of the trunk according to the cross section area of the water-guiding xylem of the trunk, and fitting the average liquid flux density as a measured value with the temperature difference between the upper probe and the lower probe inserted into the xylem of the trunk section; and correction of the empirical relationship between the average liquid flux density of the trunk and the temperature difference of the sensor probe is realized. The method is suitable for measuring and calibrating the trunk liquid flow.

The invention relates to the technical field of plant genetic engineering, in particular to a PtoNAC90 gene for regulating formation of poplar wood and application of the PtoNAC90 gene. The nucleotide sequence of the PtoNAC90 gene is as shown in SEQ ID No. 1; the amino acid sequence of the PtoNAC90 gene is as shown in SEQ ID No. 2. According to the invention, a transgenic plant is obtained by overexpressing PtoNAC90 in 741 populus tomentosa, and compared with a wild type, the transgenic populus with overexpressed PtoNAC90 has the advantages that xylem thickness and lignin content are increased, which indicates that the PtoNAC90 gene has potential application value in improvement of populus wood yield, and wood fast growth and wood property new variety improvement can be regulated and controlled by using a genetic engineering technology.

The invention discloses application and a method of a PtobZIP29 gene in regulating and controlling wood development of populus tomentosa, the PtobZIP29 gene is excessively expressed in the populus tomentosa, so that the vascular cambium division activity of the populus tomentosa is promoted, and the formation of secondary xylem is accelerated, thereby obtaining transgenic populus tomentosa germplasm with increased wood biomass and improved wood fast-growing ability. A method and a germplasm basis are provided for breeding of forest fast-growing good varieties, the wood yield and economic benefits of economic forests can be improved, and materials are provided for building of protection forests, urban and rural greening and the like.

The invention discloses a PagARF16.1 gene for regulating xylem development of poplar and application of the PagARF16.1 gene, and belongs to the technical field of biological genetic engineering. A nucleotide sequence and an amino acid sequence of a coding region of the PagARF16.1 gene are respectively shown as SEQ ID NO. 3 and SEQ ID NO. 4. The PagARF16.1 gene is knocked out by utilizing a CRISPR / Cas9 technology, and an experimental result shows that compared with a wild type poplar, the plant height of the mutant poplar is remarkably reduced, and xylem is remarkably reduced. The result shows that the poplar auxin response factor gene PagARF16.1 is a key regulatory factor for regulating xylem development. The invention provides a new technical means for regulating and controlling the xylem development of the poplar, and has an important forest tree genetic engineering application value.

The invention discloses a mulberry twig clamping and positioning method and system, belongs to the technical field of intelligent mulberry leaf picking equipment, and aims to solve the technical problems that an existing RGB camera is easily interfered by illumination, and picking damage is caused due to the fact that a demarcation point of a tender branch and an old branch is difficult to accurately recognize. According to the method and system, 500-600 nm visible light and 750-900 nm near-infrared band images are synchronously collected through a multispectral imaging device, HSV color space parameters are extracted, and a color gradient model is established; by analyzing the gradient change rate of the hue value H and the saturation degree S and combining the curvature change rate and the near-infrared reflectivity difference value, the transition boundary of the tender and old branches is accurately judged, and the three-dimensional coordinates of the clamping datum point are generated by dynamically shifting 5-15 mm towards the side of the tender branches. According to the scheme, the limitation of single hue detection is broken through, the xylem recognition capability is enhanced by utilizing multispectral information, the clamping accuracy is improved, the method is suitable for automatic operation of a top-remaining picking mode in large-scale silkworm and mulberry planting, the picking efficiency can be effectively improved, and branch damage can be reduced.

This invention discloses a key miRNA that regulates peony plant height, stem diameter, and xylem formation, and its applications. The precursor and mature sequences of this peony miRNA are shown in SEQ ID NO.1 and SEQ ID NO.2, respectively. The peony miRN82 provided by this invention can be used to regulate peony growth. Overexpression of miRN82 reduces peony plant height and stem diameter, and narrows the xylem width, indicating that miRN82 is a key miRNA regulating peony plant height, stem diameter, and xylem formation. This invention regulates peony plant height, stem diameter, and xylem thickness by overexpressing or knocking out / silencing miRN82, enabling the breeding of peony varieties with different stem heights, thicknesses, and hardnesses according to specific needs. Therefore, it has significant application value in peony molecular breeding.

The invention relates to a PagNLP8 / 9a gene for regulating xylem development of poplar and application of the PagNLP8 / 9a gene, and belongs to the technical field of plant genetic engineering and biology. The nucleotide sequence of the PagNLP8 / 9a gene for regulating and controlling the development of the xylem of the poplar is shown as a sequence 1 in a sequence table; the amino acid sequence of the expression protein of the PagNLP8 / 9a gene is as shown in the sequence 2 in the sequence table. The PagNLP8 / 9a gene is transferred into poplar 84K, and compared with a wild type, the transgenic poplar over-expressing the PagNLP8 / 9a has a phenotype with increased xylem width, which indicates that the PagNLP8 / 9a gene is the key for regulating the development of the xylem of the poplar and has important application value in the field of high-yield genetic engineering of forest trees.

The application discloses a kind of based on the growth environment multi-factor comprehensive monitoring method and system of old tree protection, including step (S1) non-invasive perception network construction: in the crown layer area of old tree deployment meteorological monitoring unit, real-time acquisition photosynthetic active radiation intensity PAR and crown layer surface temperature T c ; with trunk as center, soil sensor array is radially laid in root distribution area, and soil volume water content θ and soil water potential are monitored in layers;Micro-invasive thermal diffusion probe is installed at the breast diameter of trunk, and xylem original liquid flow flux SF raw Is continuously measured, and initial liquid flow flux SF raw Calibration is carried out;Foundation interferometric radar is erected outside the protection zone, and three-dimensional spatial distribution point cloud P r Of root is obtained by surface scanning.This application can realize more comprehensive old tree monitoring, and timely find the existing abnormality of old tree environment.

The invention provides a spore solid bacterium inoculation method. Relates to the technical field of edible mushrooms. The method comprises the following steps: S1, culture medium group preparation: selecting trees and setting a spore source culture medium group and a receptor culture medium group in groups; s2, inducing the spore source culture medium group to form a mature sporocarp, inoculating a target edible mushroom strain on the spore source culture medium group, inducing the strain to grow and forming the mature sporocarp; s3, controlling environmental parameters to promote spores to eject and fall on the receptor culture medium group; s4, the sporocarp is obtained through natural culture, the spore source culture medium group accounts for 5%-20% of the total culture medium group in volume, the surface of the receptor culture medium group in the S1 needs to be subjected to scribing treatment, the depth of a notch is 2-5 mm, the condition that the notch makes contact with the xylem is adopted as the criterion, and if the length of the tilia wood is smaller than 30 cm, scribing is not needed. The spore solid bacterium inoculation method provided by the invention has the advantages of reducing the planting cost and improving the economic benefit.

The utility model provides a shearing sampling device for preventing plants from generating aeroembolism, belongs to the technical field of plant sample sampling, and solves the technical problems that air enters a xylem conduit to form the aeroembolism at the moment of shearing in the prior art, a moisture transport channel is blocked, and leaves are dehydrated, withered and even dried off and fall off. A shear sampling device capable of preventing plants from generating aeroembolism comprises a drive-by-wire chassis, a sampling frame fixed on the drive-by-wire chassis, a supporting rod fixed on the sampling frame, a regulation and control rod connected to the supporting rod in a sliding mode, an electric hydraulic clamp fixed to the regulation and control rod, a soaking cavity formed in the sampling frame, and a net cage arranged in the soaking cavity, a first regulation and control assembly for controlling the height of the net cage is arranged in the sampling frame, a second regulation and control assembly for controlling the height of the regulation and control rod is arranged in the supporting rod, and a driving assembly for controlling the shearing head to conduct shearing action is arranged in the regulation and control rod. The plant branch cutting device has the advantages that plant branches can be cut in water, and aeroembolism is prevented.

The invention relates to the technical field of facility horticulture and plant nutrition regulation and control, and discloses a soil conditioning synergistic selenium-rich tomato cultivation method which comprises the following steps: applying nano mineral powder and a biological nano selenium nutrient solution after tomato transplanting and seedling recovering; the method comprises the following steps: carrying out physical conditioning on soil water content in a first fruit expanding period of tomatoes so as to construct a physiological premise of xylem flow blocking, and spraying a biological nano-selenium nutrient solution on leaf surfaces in the state; performing topping treatment in a time window from 12 hours to 24 hours by taking a spraying completion moment as a starting point, and physically removing a growing point at the top end of the plant; according to the method, through the synergistic effect of soil rhizosphere environment conditioning and plant physiological intervention, under the condition of not depending on a chemical chelating agent, the problem that the selenium element is transported to leaves in a one-way mode is solved, and the selenium element can be directly transported to the leaves; the efficient targeted pumping and enrichment of the selenium element from the leaves to the fruits are realized.

The water production system described herein uses subsurface temperatures to cool water to 55 degrees Fahrenheit. That geothermally cooled water is then sent down the interior of a copper column. Thermal transfer then causes the surface of the copper column to cool. In warm humid environments, condensation will form on the outside of the copper column, much like water condenses on a glass of ice water. Alternative formats include adding a pressure sleeve to the copper column, changing the column into a sphere, and for the pressure-sleeved version dropping the entire thing down a hole wherein the outer surface of the pressure sleeve comes into contact with subsurface ground temperatures cooling the pressure sleeve and creating additional cooling surface area. Alternative formats also include placing the invention inside of telephone or light poles and using artificial xylem and evapotranspiration to draw geothermally cooled water up the column.

This invention relates to the field of pest control technology and discloses an electric sprayer for controlling borers of garden trees. The sprayer includes an electric pistol body, a liquid spray bottle assembly, and a sprayer assembly. The pistol body has a power button on its handle. A battery cavity is located at the bottom of the handle, housing a battery box containing a power battery. A cover is located at the rear of the pistol body, containing a circuit board electrically connected to the power button and the power battery. A trigger button is also located on the handle, and a trigger guard is fixedly mounted on the pistol body. This invention injects the pesticide solution into the larval tunnels via electric injection. When the borer larvae come into contact with the pesticide solution or by feeding on the fibrous tissue of the tree containing the pesticide, they are poisoned and die, thus effectively achieving pest control.

The invention discloses a healing promoting agent for melon vegetable grafted seedlings. The healing promoting agent contains abscisic acid and ribulose phosphate. The effect of promoting communication of phloem and xylem of grafted seedlings can be achieved by spraying the healing promoting agent before grafting, the healing time of the melon vegetable grafted seedlings is shortened, the operation of film uncovering and film covering in the healing period is reduced, the healing period management is simplified, and the healing cost is reduced.

The invention discloses an application and a method for knocking out a DOF20 gene of poplar in NL895 to increase the wood yield, and the method comprises the following steps: introducing a DOF20 knockout plant vector into NL895 to obtain a dof20 knockout transgenic plant; the ratio of stem xylem in a knockout plant is obviously higher than that of WT, and the number of xylem layers and the thickness of secondary fiber cell walls are also obviously increased; the result shows that the xylem differentiation of the poplar stem is promoted after the DDOF20 is deleted, and the method has important significance on development and utilization of the poplar.

The present disclosure provides a method for evaluating a seedling plant character of a transgenic poplar and use of an overexpressed pagEXPA1 gene in promoting xylem development of the poplar, relating to the technical field of forest wood character determination. The method for evaluating the seedling plant character of the transgenic poplar in the present disclosure includes the following steps: S1. detecting whether or not a foreign gene is integrated into a poplar genome through PCR; S2. detecting the expression level of the foreign gene in the poplar; S3. conducting a microstructural analysis on stem vascular tissues of the transgenic poplar; and S4. separating fibers, ducts, and sieve-tube cells through fiber isolation, and staining the isolated fibers to observe the morphology and structure of the fibers, ducts, and sieve-tube cells.

The invention discloses a physical method for promoting liquidambar formosana hance to become red and application of the physical method in forest management. The physical method comprises any one of side branch thin filament cutting, side branch xylem girdling and trunk base xylem girdling. Lateral branch fine filament cutting is to select lateral branches of three-year-old sweetgum plants and tightly wind nylon fine filaments in the middles of the lateral branches for three circles; the step of girdling the xylem on the side branch is that the side branch of a triennial sweetgum plant is selected, girdling is carried out for one circle at the position 3 cm away from the base part of the side branch, the girdling width is 3 cm, and then the girdled wound is wrapped with a preservative film; the step of girdling the xylem at the base part of the trunk comprises the following steps: selecting a triennial sweetgum plant, girdling a circle at the position 10 cm away from the base part of the trunk, and wrapping the girdled wound with a preservative film. According to the method, a physical method is adopted, chemical promoting agents are not used, it can also be guaranteed that the whole forest stand grows continuously, the whole plant color changing effect is good, directional color changing of a single plant can be achieved, and the advanced, timed and positioned color changing effect is achieved.

The application discloses a solid-phase extraction agent and a preparation method and application thereof. Raw materials of the solid-phase extraction agent include cigar stems and shavings, and the mass ratio of the cigar stems to the shavings is 1:1-2. The above raw materials are all natural plants, and in addition to being rich in vascular systems and cellulose, the structure formed by xylem and phloem arranged in bundles and a variety of capillary pore structures make the pores rich, and the structure is provided with rich polar hydroxyl and hydrophobic aromatic functional groups. After being prepared as the solid-phase extraction agent, the structure can form pi-pi bonds and hydrogen bonds with benzo[a]pyrene, has strong adsorption capacity for the benzo[a]pyrene and high adsorption stability, and due to the rich pores, has good permeability. The speed of the benzo[a]pyrene in the solution after adsorption through the extraction column is faster, the adsorption enrichment and purification efficiency of the benzo[a]pyrene in the solution after adsorption is high, and the cost is low and the structure is green and environment-friendly.

The invention discloses a method for preventing and treating pear fire blight spots on a trunk of bergamot pears, which comprises the following steps: preparation of a medicament combination liquid: diluting bacillus amyloliquefaciens with clear water by 300-400 times, diluting multi-manganese zinc with clear water by 400-500 times, mixing uniformly diluted medicaments according to the weight ratio of bacillus amyloliquefaciens to multi-manganese zinc of (1-1.5): (1-1.5), and preparing a medicament mixed liquid for later use; when the trunk is attacked in June every year, aiming at pear fire disease spots on the trunk of the bergamot pear, sawing off diseased bark at the pus drainage part of the trunk by using an electric saw until the xylem of the trunk is not damaged; dipping the prepared medicament mixed solution by using a brush, uniformly smearing scabs, and smearing for the second time after one week. The method is simple, convenient and rapid, low in cost and high in operability, the control effect can reach 100% under the condition that the tree body has withered symptoms due to the disease spots on the trunk, and the control effect can be about 75% when the tree body has withered symptoms is gradually recovered or sprouts and shoots are formed.