145 results about "Xylem" patented technology

The invention provides application of an ECT2 gene in regulating and controlling development of secondary xylem of a poplar. Transgenic poplar with overexpressed ECT2 genes is obtained through genetic transformation, it is found that overexpressed ECT2 genes can increase the width of secondary xylem of the poplar, and the nucleotide sequence of the ECT2 genes is shown as SEQ ID NO: 1. The ECT2 gene has a regulation effect on the development of the secondary xylem of the poplar.

The invention discloses a high-survival-rate grafting method for grafting pinus sylvestris to pinus koraiensis in arid regions, which comprises the following steps: selecting pinus sylvestris stock with the growth period of 4-6 years, flatly cutting below a terminal bud branch point to form a cross section, downwards and obliquely cutting twice from the cross section to form a triangular pyramid-shaped chimeric structure, cutting a semi-lignified pinus koraiensis scion with a terminal bud, and grafting the semi-lignified pinus koraiensis scion with the terminal bud to obtain the pinus sylvestris scion. After main buds are reserved and lateral buds are cut off, two oblique triangular xylem sections are obliquely cut out from the lower portion of spores through two cutters, and a double-slope wedge-shaped joint body is formed; when the rootstock and the scion are cut, the cutting blade is immersed in an aqueous solution containing a plant growth regulator and a bactericide; the double-slope wedge-shaped joint body is inserted into the triangular pyramid-shaped embedding structure, so that the scion medullary layer and the stock medullary layer are overlapped; wrapping the interface with a composite hydrogel film containing a temperature-sensitive polymer and an antibacterial component; shading treatment is carried out after grafting is completed. The method can significantly improve the survival rate of grafted seedlings.

The invention discloses application of a PdeTLP protein and a related biological material thereof in regulation and control of plant root system development. Experiments prove that compared with wild poplar, the number of adventitious roots, the number of adventitious root lateral roots, the root length and the root included angle of PdeTLP knockout poplar are obviously increased, and the plant height, the number of stem nodes and the stem diameter are also obviously increased. In addition, the xylem width, the phloem width and the xylem cell area of the poplar with the PdeTLP knocked out are obviously higher than those of the wild poplar. The PdeTLP gene participates in regulation and control of the root system number, the root system length, the root system included angle, the plant height, the stem node number, the stem diameter and the secondary microtubule tissue development of the poplar. The method not only has important significance on molecular mechanism and theoretical research of regulating and controlling the number and length of poplar root systems, plant height, number of stem nodes and stem diameter, but also has important guiding significance on directionally cultivating trees meeting actual production requirements.

The invention relates to the field of agricultural bioengineering, in particular to application of an oilseed rape BnaA08G0167600WE gene in enhancing pod shattering resistance of oilseed rape. According to the invention, after a transgenic plant is constructed in oilseed rape by utilizing a modern biotechnological means and the oilseed rape gene BnaA08G0167600WE is knocked out, the silique of the mutant is not easy to crack compared with a wild type under the action of external force, and a separation layer and a wood layer of the cross section of the silique of the mutant have more cell connections compared with the wild type, so that the yield of the mutant is improved. And the generation of the mutants provides an important genetic material for further researching the function of the gene and the effect of the gene in the rape.

The invention relates to a PagNRT3.1 gene for regulating xylem development of poplar and application of the PagNRT3.1 gene, and belongs to the technical field of plant genetic engineering and biology. The nucleotide sequence of the PagNRT3.1 gene for regulating and controlling the xylem development of the poplar is shown as a sequence 1 in a sequence table; the amino acid sequence of the expression protein of the PagNRT3.1 gene is shown as the sequence 2 in the sequence table. The PagNRT3.1 gene is transferred into poplar 84K, and compared with a wild type, the transgenic poplar over-expressing the PagNRT3.1 has a phenotype with increased xylem width, which indicates that the PagNRT3.1 gene is the key for regulating and controlling the development of the xylem of the poplar and has important application value in the field of high-yield genetic engineering of forest trees.

The invention relates to the technical field of plant genetic engineering, in particular to a PtoNAC90 gene for regulating formation of poplar wood and application of the PtoNAC90 gene. The nucleotide sequence of the PtoNAC90 gene is as shown in SEQ ID No. 1; the amino acid sequence of the PtoNAC90 gene is as shown in SEQ ID No. 2. According to the invention, a transgenic plant is obtained by overexpressing PtoNAC90 in 741 populus tomentosa, and compared with a wild type, the transgenic populus with overexpressed PtoNAC90 has the advantages that xylem thickness and lignin content are increased, which indicates that the PtoNAC90 gene has potential application value in improvement of populus wood yield, and wood fast growth and wood property new variety improvement can be regulated and controlled by using a genetic engineering technology.

The invention discloses a PagARF16.1 gene for regulating xylem development of poplar and application of the PagARF16.1 gene, and belongs to the technical field of biological genetic engineering. A nucleotide sequence and an amino acid sequence of a coding region of the PagARF16.1 gene are respectively shown as SEQ ID NO. 3 and SEQ ID NO. 4. The PagARF16.1 gene is knocked out by utilizing a CRISPR / Cas9 technology, and an experimental result shows that compared with a wild type poplar, the plant height of the mutant poplar is remarkably reduced, and xylem is remarkably reduced. The result shows that the poplar auxin response factor gene PagARF16.1 is a key regulatory factor for regulating xylem development. The invention provides a new technical means for regulating and controlling the xylem development of the poplar, and has an important forest tree genetic engineering application value.

The invention discloses a mulberry twig clamping and positioning method and system, belongs to the technical field of intelligent mulberry leaf picking equipment, and aims to solve the technical problems that an existing RGB camera is easily interfered by illumination, and picking damage is caused due to the fact that a demarcation point of a tender branch and an old branch is difficult to accurately recognize. According to the method and system, 500-600 nm visible light and 750-900 nm near-infrared band images are synchronously collected through a multispectral imaging device, HSV color space parameters are extracted, and a color gradient model is established; by analyzing the gradient change rate of the hue value H and the saturation degree S and combining the curvature change rate and the near-infrared reflectivity difference value, the transition boundary of the tender and old branches is accurately judged, and the three-dimensional coordinates of the clamping datum point are generated by dynamically shifting 5-15 mm towards the side of the tender branches. According to the scheme, the limitation of single hue detection is broken through, the xylem recognition capability is enhanced by utilizing multispectral information, the clamping accuracy is improved, the method is suitable for automatic operation of a top-remaining picking mode in large-scale silkworm and mulberry planting, the picking efficiency can be effectively improved, and branch damage can be reduced.

This invention discloses a key miRNA that regulates peony plant height, stem diameter, and xylem formation, and its applications. The precursor and mature sequences of this peony miRNA are shown in SEQ ID NO.1 and SEQ ID NO.2, respectively. The peony miRN82 provided by this invention can be used to regulate peony growth. Overexpression of miRN82 reduces peony plant height and stem diameter, and narrows the xylem width, indicating that miRN82 is a key miRNA regulating peony plant height, stem diameter, and xylem formation. This invention regulates peony plant height, stem diameter, and xylem thickness by overexpressing or knocking out / silencing miRN82, enabling the breeding of peony varieties with different stem heights, thicknesses, and hardnesses according to specific needs. Therefore, it has significant application value in peony molecular breeding.

The invention relates to a PagNLP8 / 9a gene for regulating xylem development of poplar and application of the PagNLP8 / 9a gene, and belongs to the technical field of plant genetic engineering and biology. The nucleotide sequence of the PagNLP8 / 9a gene for regulating and controlling the development of the xylem of the poplar is shown as a sequence 1 in a sequence table; the amino acid sequence of the expression protein of the PagNLP8 / 9a gene is as shown in the sequence 2 in the sequence table. The PagNLP8 / 9a gene is transferred into poplar 84K, and compared with a wild type, the transgenic poplar over-expressing the PagNLP8 / 9a has a phenotype with increased xylem width, which indicates that the PagNLP8 / 9a gene is the key for regulating the development of the xylem of the poplar and has important application value in the field of high-yield genetic engineering of forest trees.

The application discloses a kind of based on the growth environment multi-factor comprehensive monitoring method and system of old tree protection, including step (S1) non-invasive perception network construction: in the crown layer area of old tree deployment meteorological monitoring unit, real-time acquisition photosynthetic active radiation intensity PAR and crown layer surface temperature T c ; with trunk as center, soil sensor array is radially laid in root distribution area, and soil volume water content θ and soil water potential are monitored in layers;Micro-invasive thermal diffusion probe is installed at the breast diameter of trunk, and xylem original liquid flow flux SF raw Is continuously measured, and initial liquid flow flux SF raw Calibration is carried out;Foundation interferometric radar is erected outside the protection zone, and three-dimensional spatial distribution point cloud P r Of root is obtained by surface scanning.This application can realize more comprehensive old tree monitoring, and timely find the existing abnormality of old tree environment.

The invention provides a spore solid bacterium inoculation method. Relates to the technical field of edible mushrooms. The method comprises the following steps: S1, culture medium group preparation: selecting trees and setting a spore source culture medium group and a receptor culture medium group in groups; s2, inducing the spore source culture medium group to form a mature sporocarp, inoculating a target edible mushroom strain on the spore source culture medium group, inducing the strain to grow and forming the mature sporocarp; s3, controlling environmental parameters to promote spores to eject and fall on the receptor culture medium group; s4, the sporocarp is obtained through natural culture, the spore source culture medium group accounts for 5%-20% of the total culture medium group in volume, the surface of the receptor culture medium group in the S1 needs to be subjected to scribing treatment, the depth of a notch is 2-5 mm, the condition that the notch makes contact with the xylem is adopted as the criterion, and if the length of the tilia wood is smaller than 30 cm, scribing is not needed. The spore solid bacterium inoculation method provided by the invention has the advantages of reducing the planting cost and improving the economic benefit.

The utility model provides a shearing sampling device for preventing plants from generating aeroembolism, belongs to the technical field of plant sample sampling, and solves the technical problems that air enters a xylem conduit to form the aeroembolism at the moment of shearing in the prior art, a moisture transport channel is blocked, and leaves are dehydrated, withered and even dried off and fall off. A shear sampling device capable of preventing plants from generating aeroembolism comprises a drive-by-wire chassis, a sampling frame fixed on the drive-by-wire chassis, a supporting rod fixed on the sampling frame, a regulation and control rod connected to the supporting rod in a sliding mode, an electric hydraulic clamp fixed to the regulation and control rod, a soaking cavity formed in the sampling frame, and a net cage arranged in the soaking cavity, a first regulation and control assembly for controlling the height of the net cage is arranged in the sampling frame, a second regulation and control assembly for controlling the height of the regulation and control rod is arranged in the supporting rod, and a driving assembly for controlling the shearing head to conduct shearing action is arranged in the regulation and control rod. The plant branch cutting device has the advantages that plant branches can be cut in water, and aeroembolism is prevented.

The invention relates to the technical field of facility horticulture and plant nutrition regulation and control, and discloses a soil conditioning synergistic selenium-rich tomato cultivation method which comprises the following steps: applying nano mineral powder and a biological nano selenium nutrient solution after tomato transplanting and seedling recovering; the method comprises the following steps: carrying out physical conditioning on soil water content in a first fruit expanding period of tomatoes so as to construct a physiological premise of xylem flow blocking, and spraying a biological nano-selenium nutrient solution on leaf surfaces in the state; performing topping treatment in a time window from 12 hours to 24 hours by taking a spraying completion moment as a starting point, and physically removing a growing point at the top end of the plant; according to the method, through the synergistic effect of soil rhizosphere environment conditioning and plant physiological intervention, under the condition of not depending on a chemical chelating agent, the problem that the selenium element is transported to leaves in a one-way mode is solved, and the selenium element can be directly transported to the leaves; the efficient targeted pumping and enrichment of the selenium element from the leaves to the fruits are realized.

The water production system described herein uses subsurface temperatures to cool water to 55 degrees Fahrenheit. That geothermally cooled water is then sent down the interior of a copper column. Thermal transfer then causes the surface of the copper column to cool. In warm humid environments, condensation will form on the outside of the copper column, much like water condenses on a glass of ice water. Alternative formats include adding a pressure sleeve to the copper column, changing the column into a sphere, and for the pressure-sleeved version dropping the entire thing down a hole wherein the outer surface of the pressure sleeve comes into contact with subsurface ground temperatures cooling the pressure sleeve and creating additional cooling surface area. Alternative formats also include placing the invention inside of telephone or light poles and using artificial xylem and evapotranspiration to draw geothermally cooled water up the column.

This invention relates to the field of pest control technology and discloses an electric sprayer for controlling borers of garden trees. The sprayer includes an electric pistol body, a liquid spray bottle assembly, and a sprayer assembly. The pistol body has a power button on its handle. A battery cavity is located at the bottom of the handle, housing a battery box containing a power battery. A cover is located at the rear of the pistol body, containing a circuit board electrically connected to the power button and the power battery. A trigger button is also located on the handle, and a trigger guard is fixedly mounted on the pistol body. This invention injects the pesticide solution into the larval tunnels via electric injection. When the borer larvae come into contact with the pesticide solution or by feeding on the fibrous tissue of the tree containing the pesticide, they are poisoned and die, thus effectively achieving pest control.

The invention discloses a healing promoting agent for melon vegetable grafted seedlings. The healing promoting agent contains abscisic acid and ribulose phosphate. The effect of promoting communication of phloem and xylem of grafted seedlings can be achieved by spraying the healing promoting agent before grafting, the healing time of the melon vegetable grafted seedlings is shortened, the operation of film uncovering and film covering in the healing period is reduced, the healing period management is simplified, and the healing cost is reduced.

The invention discloses an application and a method for knocking out a DOF20 gene of poplar in NL895 to increase the wood yield, and the method comprises the following steps: introducing a DOF20 knockout plant vector into NL895 to obtain a dof20 knockout transgenic plant; the ratio of stem xylem in a knockout plant is obviously higher than that of WT, and the number of xylem layers and the thickness of secondary fiber cell walls are also obviously increased; the result shows that the xylem differentiation of the poplar stem is promoted after the DDOF20 is deleted, and the method has important significance on development and utilization of the poplar.

The present disclosure provides a method for evaluating a seedling plant character of a transgenic poplar and use of an overexpressed pagEXPA1 gene in promoting xylem development of the poplar, relating to the technical field of forest wood character determination. The method for evaluating the seedling plant character of the transgenic poplar in the present disclosure includes the following steps: S1. detecting whether or not a foreign gene is integrated into a poplar genome through PCR; S2. detecting the expression level of the foreign gene in the poplar; S3. conducting a microstructural analysis on stem vascular tissues of the transgenic poplar; and S4. separating fibers, ducts, and sieve-tube cells through fiber isolation, and staining the isolated fibers to observe the morphology and structure of the fibers, ducts, and sieve-tube cells.

The application discloses a solid-phase extraction agent and a preparation method and application thereof. Raw materials of the solid-phase extraction agent include cigar stems and shavings, and the mass ratio of the cigar stems to the shavings is 1:1-2. The above raw materials are all natural plants, and in addition to being rich in vascular systems and cellulose, the structure formed by xylem and phloem arranged in bundles and a variety of capillary pore structures make the pores rich, and the structure is provided with rich polar hydroxyl and hydrophobic aromatic functional groups. After being prepared as the solid-phase extraction agent, the structure can form pi-pi bonds and hydrogen bonds with benzo[a]pyrene, has strong adsorption capacity for the benzo[a]pyrene and high adsorption stability, and due to the rich pores, has good permeability. The speed of the benzo[a]pyrene in the solution after adsorption through the extraction column is faster, the adsorption enrichment and purification efficiency of the benzo[a]pyrene in the solution after adsorption is high, and the cost is low and the structure is green and environment-friendly.

The invention discloses a method for preventing and treating pear fire blight spots on a trunk of bergamot pears, which comprises the following steps: preparation of a medicament combination liquid: diluting bacillus amyloliquefaciens with clear water by 300-400 times, diluting multi-manganese zinc with clear water by 400-500 times, mixing uniformly diluted medicaments according to the weight ratio of bacillus amyloliquefaciens to multi-manganese zinc of (1-1.5): (1-1.5), and preparing a medicament mixed liquid for later use; when the trunk is attacked in June every year, aiming at pear fire disease spots on the trunk of the bergamot pear, sawing off diseased bark at the pus drainage part of the trunk by using an electric saw until the xylem of the trunk is not damaged; dipping the prepared medicament mixed solution by using a brush, uniformly smearing scabs, and smearing for the second time after one week. The method is simple, convenient and rapid, low in cost and high in operability, the control effect can reach 100% under the condition that the tree body has withered symptoms due to the disease spots on the trunk, and the control effect can be about 75% when the tree body has withered symptoms is gradually recovered or sprouts and shoots are formed.

This invention discloses a vascular bundle-targeted bio-selenium nanoparticle, its preparation method, and its application, belonging to the field of plant treatment technology. The invention involves mixing and dissolving bio-selenium nanoparticles, water, 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, and N-hydroxysuccinimide, followed by activation to obtain an activated bio-selenium nanoparticle solution. A xylem-anchored peptide and MES buffer are then mixed and dissolved to obtain a xylem-anchored peptide solution. The activated bio-selenium nanoparticle solution and the xylem-anchored peptide solution are mixed, and the pH is adjusted to 6.3-6.8 before stirring to obtain the vascular bundle-targeted bio-selenium nanoparticle. The vascular bundle-targeted bio-selenium nanoparticle provided by this invention achieves interception of heavy metal transport pathways by specifically anchoring to xylem vessels. Compared with existing technologies that rely solely on broad-spectrum physiological antagonism, its efficiency in reducing heavy metal content in crop stems and leaves represents a qualitative leap.

The application discloses a kind of karst region crop precision irrigation methods based on stem sap flow early warning, comprising: step 1, layout underground infiltration irrigation system drip irrigation pipeline and surface water retaining layer;Step 2, install heat pulse sap flow sensor on healthy planting pearl stem;Step 3, according to the early warning signal generated by heat pulse sap flow sensor sap flow rate, as the starting signal of infiltration irrigation system;Step 4, after starting infiltration irrigation system, start underground infiltration irrigation system to carry out targeted water replenishment, when the monitored real-time average sap flow rate falls to normal level, close irrigation valve, complete closed-loop irrigation;Realize the combination of karst mountainous area drought early warning and root closed-loop targeted irrigation, effectively avoid the death of plant due to catastrophic hydraulic failure in xylem, significantly improve water resource utilization efficiency and crop drought survival rate;Solve the technical problems such as irrigation timing lag and low water resource utilization efficiency in karst region.

The application relates to the technical field of detection, in particular to a preparation method of sapwood section of Picea smithiana wood; the method comprises the following steps: fixing the sapwood sample of Picea smithiana after trimming in a fixing solution, softening the fixed sapwood sample of Picea smithiana in an ethylenediamine solution for 5-6 days, slicing the sapwood sample of Picea smithiana after the softening is completed, bleaching, washing and dyeing and eluting the sapwood section of Picea smithiana, making the section of the sapwood sample of Picea smithiana transparent by using an ethanol and xylene mixed solution, and sealing and shooting the section of the sapwood sample of Picea smithiana; the application increases the contact area of the sample with the FAA solution and the ethylenediamine solution, accelerates the fixing and softening speed of the sample, shortens the softening time from one month to 5-6 days, and realizes the rapid and efficient preparation of the sapwood section of Picea smithiana wood.

The present invention relates to a method for rapidly and efficiently extracting a plant essential oil from a plant raw material, particularly from xylem of a pine tree, thereby maximizing the yield, reducing the manufacturing cost and time, improving the productivity, performing high-purity treatment on the extracted plant essential oil by a refining process, thereby realizing the diversification and commercialization of the extracted oil, and improving the quality of the plant essential oil. The invention relates to a plant essential oil extraction method and plant essential oil extracted oil extracted by the extraction method.

The application discloses a method for preventing and treating trunk pear fire blight spots of fragrant pear, which comprises the following steps: a medicament mixed solution is prepared by mixing bacillus amyloliquefaciens diluted 300-400 times with water and manganese zinc oxide diluted 400-500 times with water in a weight ratio of 1-1.5:1-1.5; when the trunk of the fragrant pear is diseased in June every year, diseased tree bark at a trunk pus flow is cut off by using an electric saw without damaging the trunk wood; the prepared medicament mixed solution is evenly applied to the disease spots by using a brush, and the second application is performed after one week. The method is simple, fast, low in cost and high in operability, the prevention effect can reach 100% when the trunk disease spots have not caused the trunk to appear the dead symptoms, and the prevention effect can reach about 75% when the trunk has appeared the dead symptoms, the trunk can gradually recover, new buds can be generated, and new branches can be formed.

The present invention belongs to the technical field of extraction and purification of fat-soluble chemical components, and particularly relates to a method for extracting and purifying dihydroquercetin from larch xylem. The present invention uses tea saponin aqueous micelles (tea saponin aqueous solution) to greenly extract dihydroquercetin from larch xylem, and then, according to the interfacial charge characteristics of the micelles formed by tea saponin and dihydroquercetin in the aqueous solution, utilizes the charge characteristics of chitosan to directionally enrich dihydroquercetin in the extract, and combines anti-solvent biphasic crystallization and water-solvent single-phase recrystallization to purify dihydroquercetin. The extraction and purification method provided by the present invention has a high extraction rate of dihydroquercetin and a high purity of the obtained pure product, and has the characteristics of simple operation, green safety, high efficiency and low cost. It not only improves the utilization rate of larch resources, but also has great significance for giving play to the high-value application of dihydroquercetin.

The invention belongs to the technical field of planting, and particularly relates to a method and application for promoting cutting rooting of cubilose fruits, and the method comprises the following steps: obliquely cutting the base of a stem section of the cubilose fruits to remove meat, and exposing xylem by 3-5cm to obtain cuttings; airing the cuttings for more than 7 days, performing cuttage, and applying a composite rooting agent to obtain cutting seedlings; the composite rooting agent comprises the following effective components: a nitrogen-potassium compound fertilizer, humic acid, a seaweed extract and chitosan. According to the method disclosed by the invention, the base part of the stem section is obliquely cut to form an inclined cut, so that the contact surface of the callus is increased, and more adventitious roots are promoted to germinate; meanwhile, cutting is conducted after the cuttings are aired for 7 days or above, a composite rooting agent is applied, rooting of the cuttings can be promoted, the rooting rate is remarkably increased, the grown roots are long, thick and strong, and the survival rate of cutting seedlings is also remarkably increased.

This invention relates to the field of biotechnology, specifically disclosing a method for improving the biocompatibility of poplar trees. PagBEH3b Methods for promoting poplar lignite development and / or increasing poplar height and / or increasing poplar yield through gene expression. This invention involves... PagBEH3b Genes were transferred into poplar trees and overexpressed. PagBEH3b Compared with wild-type, transgenic poplar showed a significant increase in xylem, indicating that the xylem of the brassinolide signaling pathway is a key regulator of this pathway. PagBEH3b It is a key regulatory factor in controlling xylem development in poplar. This invention provides a new option for regulating xylem development and has significant application value in the field of forest tree genetic engineering.

The application discloses a kind of saline-alkali soil crops stem xylem water potential prediction method and related equipment.The method comprises: using cloud migration optimization algorithm to optimize nonlinear Arp algorithm parameters, and cloud migration optimization algorithm and nonlinear Arp algorithm are constructed into integral cloud migration optimization-nonlinear Arp algorithm;Based on the stem xylem water potential data set collected, the crop stem xylem water potential model constructed by cloud migration optimization-nonlinear Arp algorithm is trained, and SHAP interpreter is used to optimize input factor;Based on the trained crop stem xylem water potential model, the saline-alkali soil crop stem xylem water potential is predicted.It can solve the problem that the traditional soil moisture or soil water potential determination method can only determine soil matric potential, it is difficult to determine the superposition water potential of osmotic potential and matric potential, and it is more difficult to accurately determine the time when crop physiological drought occurs.