43 results about "Pestalotiopsis sp." patented technology

The invention discloses a method for manually inducing Aquilaria sinensis to generate agilawood. The method comprises the following steps of: injecting a methanoic acid or acetic acid solution with pH value of 1.5-3 or a mixed solution of methanoic acid and acetic acid into xylem of Aquilaria sinensis [Aquilaria sinensis (Lour.) Gilg]; respectively injecting the solution together with fungi, suchas Botryosphaeria rhodina, Hypocrea jecorina, Fusarium sp., Colletotrichum gloeosporioides, Ampelomyces sp, Khuskia sp, Fungal endophyte sp.A 16, Pestalotiopsis sp., Hypocrea lixii and Chaetomium sp.or injecting the solution and the fungi in a mixed way; and inducing the Aquilaria sinensis to generate the agilawood. According to the invention, the methanoic acid or acetic acid solution or the mixed solution of methanoic acid and acetic acid is injected into the xylem of the Aquilaria sinensis to induce the Aquilaria sinensis to generate defense reaction so as to generate the agilawood; and alarge amount of agilawood are generated by using the method. The method for manually inducing the Aquilaria sinensis to generate the agilawood, disclosed by the invention, has the advantages of simplicity and convenience in operation, low cost and suitability for manually making agilawood on a large scale by using Aquilaria sinensis trees; and a large amount of agilawood can be effectively generated by the Aquilaria sinensis. According to the invention, the agilawood producing period of the Aquilaria sinensis is largely reduced, the agilawood can be generally obtained after 6-24 months, the production cost is reduced, the market requirements are satisfied, and important economic, social and ecological benefits are obtained in aspects of protection and sustainable utilization of the Aquilaria sinensis, economic development of mountainous regions and the like.

The invention discloses dendrobium officinale Kimura et Migo endophytic fungus. The dendrobium officinale Kimura et Migo endophytic fungus is obtained by separating from the living body of dendrobium officinale Kimura et Migo belonging to dendrobium species, orchidaceae by adopting an endophytic fungus separation and purification technology; and identified by the microbial taxonomy, the dendrobium officinale Kimura et Migo endophytic fungus is Pestalotiopsis sp.DO14; the strain is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number CCTCC M 2015180. Through co-culture with dendrobium officinale Kimura et Migo tissue culture seedlings, the steam thickening and lengthening, and the stem and leaf color deepening of the dendrobium officinale Kimura et Migo tissue culture seedlings can be promoted, the improvement of the polysaccharide content in the stem can be promoted, the change of alcohol soluble ingredients in the stem and the flavonoid ingredients in the leaves can be promoted; the dendrobium officinale Kimura et Migo endophytic fungus is an important microbe closely related to the growth and metabolites of dendrobium officinale Kimura et Migo, and has the application potential of promoting the growth of dendrobium officinale Kimura et Migo and the change of the metabolic components.

The invention discloses a bacillus polymyxa strain and application thereof. The Bacillus polymyxa strain of the present invention, the Bacillus polymyxa strain is PBSZ123, the preserved name is Bacillus polymyxa (Paenibacillus polymyxa), and it is preserved in the General Microorganism Center (CGMCC) of China Microorganism Culture Preservation Management Committee, the depository unit The address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. Date of deposit: August 14, 2019; Collection number: CGMCC No.18386. The bacillus polymyxa of the present invention is isolated from the endophyte of the polyphylla, and has a good inhibitory effect on the main pathogenic bacteria of the polyphylla, Botrytis cinerea, Phoma leaf spot, and Polychaete spp.

The invention discloses a strain of Penicillium breenii and its application. The Penicillium brefeldianum strain of the present invention, the Penicillium brefeldianum strain is PFLJ04, and the preservation name is Penicillium brefeldianum, which is preserved in the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee, and the depository address is No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, date of deposit: August 15, 2019; deposit number: CGMCC No.18119. The penicillium of the present invention is isolated from the endophytic bacteria of P. diangi, and can simultaneously treat notoginseng root rot pathogens (Fusarium oxysporum, Fusarium solani, Phytophthora spp.) The pathogens (Botrytis cinerea, Phoma leaf spot, and Polychaete spp.) have good inhibitory effect.

The invention relates to the technical field of microorganisms. An artemisia japonica endophytic fungus is collected from living bodies of artemisia japonica from a suburb of Guiyang city in Guiyang province through endophytic fungus separation technology. The artemisia japonica endophytic fungus is named pestalotiopsis uvicola GMH 31 through authentication by microbial taxonomy. The strain is deposited in China center for type culture collection with a deposition date being June-30-2014 and an accession number being CCTCC No: M2014303. The invention has following most significant characteristics: (1) A fermentation liquid of the strain is an active metabolite which has inhibiting effects of sclerotinia sclerotiorum, phytophthora capsici and the like and is an important microbial resource; and (2) A fermentation mycelium of the strain can secret various natural active components, has medicinal effects of brad-spectrum tumor resistance and the like, and is an important microbial resource of a novel natural active drug.

The invention relates to a compound as well as a preparation method and an application thereof, in particular to a preparation method of a novel medicament for inhibiting pestalotiopsis, peronophythora litchii and cylindrocladium parasiticum. Naphthylacetic acid technical, bromoethanol technical and carbendazim technical as synthesis raw materials are subjected to a reaction, and naphthylacetic acid-carbendazim conjugate is obtained and is the final product. The invention also discloses nuclear magnetic resonance structural data, hydrogen spectra and carbon spectra of the novel compound. The compound has good bacteriostatic effects on pestalotiopsis, peronophythora litchii and cylindrocladium parasiticum.

The invention belongs to the technical field of microbes, and discloses a fungus of the genus Discotrichum spp. and its application, that is, through the separation of the endophytic fungus of Cordyceps sinensis and the analysis of the change in properties, different mediums are used to cultivate, separate and purify mole cricket bodies and claws , and isolated and screened a fungus of the genus Discotrichum spp., which was preserved in the Guangdong Microbial Culture Collection Center on December 26, 2016, with the preservation number GDMCC No: 60135. The fungus grows best under conditions of rice culture medium, 20°C, and no light, and can produce high content of nucleosides. The discovery of this fungus is expected to realize the mass fermentation and production of nucleosides in vitro, and has a wide range of applications value.

The invention relates to the preparation domain of plant source pesticide, especially a plant source bactericide and artificial synthesis method thereof, in order to solve that the essential oil in marigold is used as plant source bactericide and applied in plant pathogen. The bactericide is 2,4-bicycloalkene-pentane-cyclopentanone the formula is as figure, firstly the cyclopentanone is heated alkalinity condition to generate 2-bicycloalkene-pentane-cyclopentanone, and then the 2-bicycloalkene-pentane-cyclopentanone reacts with cyclopentanone to generate 2,4-bicycloalkene-pentane-cyclopentanone in the presence of catalyst; the water layer, oil layer are removed by the distillation way to collect volatile component to obtain 2,4-bicycloalkene-pentane-cyclopentanone. The plant source bactericide has features of simple operation method, mild reaction condition, high yield, suitable for large-scale industrialization production, more important, the plant source bactericide is applied for the plant source pesticide, with killing function to the plant pathogen such as watermelon, pepper wilt, Pestalotiopsis sp, Sclerotinia sclerotiorum, with high inhibition rate and provides new path of the development of Chinese plant source pesticide.