18 results about "Pestalotiopsis sp." patented technology

The invention provides a trichoderma Guizhou B10 strain and application thereof, and relates to the technical field of agricultural microbial control. The trichoderma Guizhou B10 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No. 3.25634. The invention overcomes the defects in the prior art, and the strain can inhibit pathogenic fungi of malania oleifera leaf spot disease, such as pestalotiopsis, pestalotiopsis, clostridium neocarpum, botryosphaeria and physophaga fungi, can promote the growth of malania oleifera plants, and has both disease-resistant and growth-promoting functions on malania oleifera seedlings, such as malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, and malania oleifera leaf spot disease resistance.

The invention belongs to the technical field of plant disease prevention and treatment, and particularly relates to a biocontrol bacterium for preventing and treating idesia anthracnose, fermentation liquor, a biocontrol bacterium agent and application. According to the invention, a biocontrol bacterium is separated and screened from microbial resources on healthy idesia leaves, is classified and named as Bacillus velezensis, is preserved in China General Microbiological Culture Collection Center on March 28, 2025, and has a preservation number of CGMCC No.34038; the biocontrol bacterium provided by the invention has a good antagonistic effect on colletotrichum causing idesia anthracnose, melanospora causing idesia leaf spot, botrytis cinerea, fusarium oxysporum, alternaria alternata and pestalotiopsis which can cause plant diseases, and also has a good antagonistic effect on colletotrichum causing idesia anthracnose, melanospora causing idesia leaf spot, fusarium oxysporum, alternaria alternata and pestalotiopsis. The biocontrol bacterium, the fermentation broth and the biocontrol inoculant provided by the invention have potential application prospects in prevention and treatment of idesia anthracnose.

The present application belongs to the technical field of plant disease prevention and treatment, and particularly relates to a biocontrol bacterium for preventing and treating Guaiacum officinale anthracnose, a fermentation liquor, a biocontrol bacterium agent and application. Bacillus velezensis A biocontrol bacterium is isolated and screened from a microbial resource on a healthy Guaiacum officinale leaf, and is classified and named as Bacillus velezensis , which is preserved in the China General Microbiological Culture Collection Center on March 28, 2025, and has a preservation number of CGMCC No.34038; the biocontrol bacterium provided in the present application has good antagonistic effects on Colletotrichum gloeosporioides causing Guaiacum officinale anthracnose, on Stemphylium botryosum causing Guaiacum officinale leaf spot disease, on Botryosphaeria dothidea, on Alternaria alternata and on Pestalotiopsis versicolor capable of causing plant diseases. The biocontrol bacterium, the fermentation liquor and the biocontrol bacterium agent provided in the present application have potential application prospects in preventing and treating Guaiacum officinale anthracnose.

The invention discloses a parasitic wasp-friendly multifunctional trichoderma asperellum ZJ12 and application thereof, the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M2025805, and the strain has broad-spectrum and efficient antagonistic activity; various phytopathogens such as fusarium oxysporum, fusarium solani, fusarium pseudograminearum, verticillium dahliae, tea pestalotiopsis, phytophthora nicotianae, northern leaf spot helminthosporium bombycis and globulosporium melanocarpum are efficiently inhibited, and a good prevention and treatment effect is shown in a potting prevention effect experiment; the biocontrol microbial inoculum shows transboundary prevention and control potential to lepidoptera pests (plutella xylostella and cabbage caterpillars) and has good biological safety to natural enemy insect parasitic wasps (chrysalis pupae and lateral cotesia manilensis), and the problem that an existing biocontrol microbial inoculum is single in function is effectively solved.

The invention discloses an application of fluoxastrobin (Fluoxastrobin) in prevention and treatment of pathogenic fungi of crops. The fluoxatrobin has excellent bacteriostatic activity on pathogenic fungi of crops, can be used for preventing and treating diseases of important economic crops such as tea trees, vegetables, rice, fruit trees and the like caused by the pathogenic fungi, and particularly has a remarkable bacteriostatic effect on pestalotiopsis trachichioides (Pestalotiopsis trachichioides), and can be used for preventing and treating the diseases of the important economic crops such as the tea trees, the vegetables, the rice, the fruit trees and the like caused by the pathogenic fungi of the important economic crops such as the tea trees, the vegetables, the rice, the fruit trees and the like. The fluoxastrobin disclosed by the invention can delay the generation of drug resistance of germs, expand the bactericidal spectrum of pesticides and reduce the pollution of the pesticides to the environment, conforms to the policy of pesticide reduction and synergism in China, and has a wide application prospect in the field of crop disease control.

The invention discloses antibacterial protein from bacillus amyloliquefaciens BS-3 and application of the antibacterial protein, and belongs to the technical field of microorganisms and genetic engineering. The invention discloses a bacteriostatic protein from bacillus amyloliquefaciens BS-3. The amino acid sequence of the bacteriostatic protein is as shown in SEQ ID NO. 7. The antibacterial protein from the bacillus amyloliquefaciens BS-3 can inhibit colletotrichum acutatum, alternaria rubra and pestalotiopsis microspora.

The application discloses application of cembrene in inhibition of a disease development process of a tea tree annular spot, and belongs to the technical field of tea tree annular spot prevention and treatment. In vitro, standard cembrene and Pestalotiopsis funerea mycelium blocks are co-cultured, the MIC50 value is calculated to measure the bacteriostatic effect, and when the cembrene is co-cultured with a colletotrichum genus fungus, it is also found that the cembrene has a good inhibitory effect on mycelium growth. Meanwhile, it is also found that the cembrene can damage Pestalotiopsis funerea spore cells and affect the pathogenicity of the Pestalotiopsis funerea to tea tree leaves. Compared with traditional bacteriostatic agents, the application provides a more effective and environment-friendly bacteriostatic agent for inhibiting plant annular spot in the market.

The invention discloses a biocontrol bacillus amyloliquefaciens WYP1-4 for degrading feathers and application thereof, and belongs to the technical field of microorganisms. The bacterial strain is classified and named as bacillus amyloliquefaciens WYP1-4, and is preserved in the Guangdong Microbiological Culture Collection Center, the preservation number is GDMCC No.65114, and the preservation address is the 5th floor, No.59 floor, Institute of Microbiology, Academy of Sciences, Guangdong Province. The feather degradation rate of the strain reaches 93% (28 h), and the strain has high industrial utilization value and has a good antagonistic effect on tea tree pathogenic bacteria pestalotiopsis pseudomonas CLBB1 and aspergillus niger YC01.

The invention discloses application of a PmchoC gene in prevention and treatment of kiwi fruit soft rot, compared with a wild type, after the PmchoC gene of pestalotiopsis microspora is knocked out, the integrity of a cell wall is obviously reduced, the sporulation quantity and the germination rate are obviously reduced, and the diameter of a scab is obviously reduced after the pestalotiopsis microspora is inoculated to kiwi fruits; the gene is proved to be capable of directly influencing the pathogenicity of pestalotiopsis microspora; by spraying the dsRNA of the PmchoC gene, the diameter of the scab of the kiwi fruit is reduced by 55.26%, the diameter of the scab of the leaf is reduced by 67.88%, and the PmchoC-dsRNA relieves the disease symptom of the soft rot of the kiwi fruit by inhibiting the expression of the PmchoC gene in pestalotiopsis microspora. According to the method, a spraying-induced gene silencing technology is applied to prevention and treatment of the soft rot of the kiwi fruit for the first time, and the problems that traditional disease-resistant breeding is long in period, resistance is prone to loss, and the disease outbreak risk is large are solved.

The invention provides a kiwi fruit fresh-keeping biocontrol bacterium and application thereof, the bacterial strain is classified and named as Bacillus amyloliquefaciens BacXF14, the preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) NO: 35691, and the preservation date is August 20, 2025. The bacterial strain has bacteriostatic activity on botryosphaeria dothidea, alternaria alternata, phomopsis, phomopsis vitis, botrytis cinerea and pestalotiopsis. The strain can effectively inhibit the growth of various pathogenic bacteria after harvesting of kiwi fruits, and the occurrence of soft rot caused by botryosphaeria dothidea infection is remarkably reduced. The composition of microbial community structures on the surfaces of the kiwi fruits can be regulated and controlled, the relative abundance of spoilage pathogenic bacteria is reduced, and the relative abundance of beneficial microorganisms is increased, so that the quality of the kiwi fruits is improved, and the preservation period of the fruits is prolonged. In conclusion, the strain has wide application potential and value in the postharvest preservation process of the kiwi fruits.

The invention discloses a microspore pseudodisk multi-hairy spore for promoting the growth of Dendrobium officinale and its application. The classification name is Pestalotiopsis microspora, the classification position is Ascomycota, Coelosporales, Black disc order (family), and the genus Pestalotiopsis. The strain number is JSNLSHGS‑13, and it has been preserved in the General Microbiological Center of China Microbiological Culture Collection Committee, with a preservation number of CGMCC NO.18543 and a preservation date of September 2, 2019. The microspore pseudodisk multi-hairy spore Pestalotiopsis microspora strain JSNLSHGS‑13 of the present invention has a significant effect on promoting the growth of Dendrobium officinale seedlings, and has a certain effect on reducing the application of chemical fertilizers; at the same time, it can be artificially cultivated, which is convenient for large-scale production and has good development and application prospects.

The application belongs to the technical field of crop disease detection, identification and prevention, and particularly relates to specific primers for detecting Pestalosphaeria theae and application thereof. The specific primers for detecting Pestalosphaeria theae provided by the application comprise SEQ ID NO. 1 and SEQ ID NO. 2. The specific primers provided by the application can detect 0.04 ng of Pestalosphaeria theae DNA, can specifically distinguish Pestalosphaeria theae from other fungal species on tea leaves, and can be directly applied to field detection. The application of the application to detection of Pestalosphaeria theae has the advantages of strong specificity, short detection time, good stability, wide applicability, simplicity, rapidness, low cost and the like, and can be effectively used for early diagnosis of tea Pestalosphaeria theae and monitoring and identification of Pestalosphaeria theae.

Disclosed in the present invention is a use of a 2-cyano-3-aminoacrylate compound in control of fungal diseases caused by Pestalotiopsis-like fungi. The compound exhibits generally ultrahigh ex-vivo antifungal activity against Pestalotiopsis-like fungi, including Pestalotiopsis, Neopestalotiopsis, and Pseudopestalotiopsis. Phenamacril exhibits a median effective concentration (EC50) ranging from 0.1455 to 0.6025 μg / mL, a mean value of 0.2800 μg / mL and a standard deviation of 0.095 μg / mL against 56 strains of Pestalotiopsis-like pathogenic fungi isolated from Camellia sinensis, Prunus persica, and Vitis vinifera. The Phenamacril suspension formulation spray treatment scheme for Camellia sinensis provided by the present invention can significantly increase tea yield, and provide a basis for controlling diseases caused by Pestalotiopsis, Neopestalotiopsis, and Pseudopestalotiopsis pathogens in tea plantations as well as for monitoring fungicide resistance.

This invention provides a *Trichoderma guiyuan* strain B10 and its applications, relating to the field of agricultural microbial control technology. The *Trichoderma guiyuan* strain B10 is deposited at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 3.25634. This invention overcomes the shortcomings of existing technologies. This strain can inhibit the pathogenic fungi *Neoplasmosis polychaete*, *Neoplasmosis polychaete*, *Neoplasmosis spp.*, *Botrytis cinerea*, and *Metacarpus* spp. of garlic leaf spot disease, while simultaneously promoting the growth of garlic plants, exhibiting both disease resistance and growth-promoting functions in seedlings.

The invention belongs to the technical field of antibacterial agents, particularly relates to preparation and application of a copper N-acetylglycinate antibacterial agent, and aims to provide preparation and application of an antibacterial agent taking copper N-acetylglycinate as an active component. The copper N-acetylglycine antibacterial agent prepared by the invention has the advantages of strong antibacterial effect, wide antibacterial spectrum and the like. Biological activity experiment determination shows that the prepared N-acetylglycine copper antibacterial agent has an efficient antibacterial effect on botanical pathogenic bacteria such as pestalotiopsis, strawberry fusarium wilt, rhizoctonia solani, fusarium moniliforme and pseudomonas solanacearum, and can be used as an agricultural bactericide to be applied to agricultural production.

The invention provides a chromone compound with anti-inflammatory activity as well as a preparation method and application thereof. According to the invention, the endophytic fungi Phomopsis asparagi DHS-48 and Pestalotiopsis sp.HHL101 in mangrove forest are subjected to co-culture, and a new chromone compound Phaseolorin J is separated from the co-culture fermentation extract of the endophytic fungi Phomopsis asparagi DHS-48 and Pestalotiopsis sp.HHL101. The structural formula of the chromone compound is shown in the formula (1), the chromone compound is named as Phaseolorin J: # imgabs0 #, it is found through research that Phaseolorin J can play a role in treating RAW 264.7 macrophage inflammatory response stimulated by LPS, and the chromone compound Phaseolorin J can be applied to preparation of anti-inflammatory drugs.

The application discloses an arecoline ester derivative, a preparation method and application thereof, relates to the technical field of chemical pesticides, and has a brand-new molecular structure, and the bacteriostatic activity and insecticidal activity of the arecoline ester derivative are significantly enhanced compared with arecoline; the arecoline ester derivative has certain inhibiting effects on Puccinia recondita, Diplodia natalensis, Gibberella saubinetii, Pyricularia grisea, Phomopsis vexans, Physalospora piricola, Pestalotiopsis versiflorea, and Colletotrichum gloeosporioides; and the arecoline ester derivative also has certain insecticidal activity on Aedes aegypti larvae.

The invention relates to application of RPB1-M15 protein in prevention and treatment of plant diseases, the protein is separated from fermentation supernate of talaromyces aureoflavus and is used for inhibiting at least one of fusarium oxysporum, fusarium moniliforme and pestalotiopsis neoplasm, and then the RPB1-M15 protein can be used for inhibiting at least one of the fusarium oxysporum, the fusarium moniliforme and the pestalotiopsis neoplasm through the bacteriostatic action on the fusarium oxysporum, the fusarium moniliforme and the pestalotiopsis neoplasm. The RPB1-M15 protein can be used for preventing and treating tomato fusarium wilt, rice bakanae disease and strawberry leaf spot disease, has an ideal prevention and treatment effect on the plant diseases, has no pollution to the environment and no toxicity to human and livestock, and can be used for preparing medicines for preventing and treating various plant diseases caused by fusarium oxysporum, fusarium moniliforme and / or pestalotiopsis neoplasm.