23 results about "Camellia sinensis" patented technology

This invention discloses the tea plant miRNA miR408-p5 and its application and method in improving plant disease resistance. By constructing a silencing expression vector for miR408-p5 and transferring it into tobacco, transgenic tobacco plants were obtained through stable genetic transformation, exhibiting strong fungal inhibitory activity against *Botrytis cinerea*. Conversely, by constructing an overexpression vector for miR408-p5 and transferring it into tobacco, transgenic tobacco plants were obtained through stable genetic transformation, showing significantly weakened resistance to *Botrytis cinerea*. Therefore, silencing miR408-p5 expression can improve plant disease resistance, and introducing the miR408-p5 silencing expression vector into plants such as tobacco, tea, or vegetables to enhance plant disease resistance has broad market application prospects.

The utility model discloses a kind of temperature control rocking machine for Meiren tea making green process, specifically related to tea making equipment field.The temperature control rocking machine for Meiren tea making green process provided by the utility model, including rack, rocking barrel, temperature control device, variable frequency driving device, the rocking barrel includes stainless steel cylinder and bamboo splint cylinder, the bamboo splint cylinder is set in the stainless steel cylinder, the temperature control device is set in the stainless steel cylinder is used to adjust the temperature in the stainless steel cylinder to simulate natural day and night temperature difference, the variable frequency driving device is set on the rack, the variable frequency driving device is connected with the bamboo splint cylinder and is used to drive the bamboo splint cylinder variable frequency rotation to dynamically adjust rocking strength.

The present disclosure provides a process for obtaining a concentrated extracellular vesicles suspension. The method can include fermenting tea (camellia sinensis) with a combination of at least one yeast and bacterium to obtain a fermentation product comprising a liquid material; filtering the fermentation product through a filtering device to give a permeate; filtering the permeate through a second filtering device to give a suspension comprising extracellular vesicles; and recovering the suspension of extracellular vesicles.

The application discloses a synergistic whitening atomized liquid based on multi-source plant extracts and a preparation method and application thereof, and relates to the technical field of cosmetics. The synergistic whitening atomized liquid comprises the following components in percentage by mass: 0.1%-5.0% of a plant synergistic whitening composition, 0.5%-3.0% of a penetration enhancer, 5.0%-10.0% of a humectant, 0.05%-0.2% of a thickening agent, 0.1%-0.6% of a preservative, a pH regulator, and the balance of a solvent. The preparation method comprises the following steps: obtaining the plant synergistic whitening composition by compounding, drying, crushing and screening extracts of Sanqi, Yunnan Camellia sinensis, Spilanthes acmella and Ficus tikoua; and then preparing the synergistic whitening atomized liquid by phase separation mixing, dissolving, adjusting the pH and filtering. The plant synergistic whitening composition covering the melanin metabolism is formed by compounding, the stability problem of various plant active ingredients in the compounding and atomization processes is solved, the transdermal absorption efficiency of the active ingredients is remarkably improved by using the atomization technology, and a new scheme is provided for developing a high-efficiency natural whitening product.

This invention relates to the field of molecular biology, and more specifically, to a novel application of *Tea glomerata* exovesicles, namely, their application in the preparation of anti-inflammatory drugs, specifically in drugs for treating acute pneumonia. The method for preparing *Tea glomerata* exovesicles includes the following steps: Fresh *Tea glomerata* slices are juiced, and the juice is collected. The juice is centrifuged sequentially, and the supernatant from the third centrifugation is collected and centrifuged at 120,000-140,000g for 50-60 minutes to obtain a crude extract. The crude extract is added to a gradient concentration sucrose solution, centrifuged, and a solution containing 30%-45% bands is collected. This solution is then centrifuged at 150,000-160,000g for 50-60 minutes to obtain the *Tea glomerata* exovesicles. The method of this invention can yield *Tea glomerata* exovesicles that meet standards and have stable quality.

This invention belongs to the field of tea cultivation technology, specifically relating to the delayed flowering function of the tea tree gene CsCIP1 and its application. This invention discovered a sequence CsTGY12G0001995 homologous to the Arabidopsis thaliana AtCIP1 gene in tea trees, which was named CsCIP1. Overexpression of this gene in Arabidopsis thaliana resulted in transgenic plants flowering later than the wild type, demonstrating that this gene has a delayed flowering function and providing a candidate gene for the targeted breeding of delayed-flowering tea varieties. The functional analysis of the tea tree gene CsCIP1 and its delayed flowering provides important genetic resources and strong theoretical support for improving tea varieties in agricultural production, and has broad application value.

The utility model relates to the field of tea-oil tree seedling grafting equipment, concretely relates to a cutting device for stock of tea-oil tree seedling grafting, including tool rest, the upper end of tool rest is provided with clamping screw rod, the outside one side of clamping movable bearing is provided with clamping movable bearing, the both sides of clamping movable bearing are provided with holding arm, the one end of holding arm is provided with clamping tool, the one side annular vertical through -going of clamping tool front end is provided with pivot, the bottom of pivot is provided with anti -drop piece, the upper and lower ends of pivot are provided with resistance piece, the upper end of clamping tool front end is provided with limit frame, the utility model, through clamping screw rod, clamping movable bearing, holding arm, clamping tool constitute the clamping system of clamping tool, cooperate pivot, resistance piece, anti -drop piece, realize the stable clamping of the branch of stock, can adjust resistance piece angle and adapt to different branches, guarantee the stable position of stock in the cutting process, provide the basis for accurate cutting, improve the reliability of tea-oil tree seedling grafting stock cutting.

This invention discloses a water-oil dual-phase synergistic anti-fibrotic comprehensive formulation framework and its application based on Camellia chrysantha and Aristolochia debilis, belonging to the field of ethnic Zhuang medicine biomedicine and herbal chronic disease management technology. This invention is the first to fix Camellia chrysantha and Aristolochia debilis as the unique dual-core framework, creating a novel dual-phase synergistic underlying pharmacology of water-phase flavonoids against organ fibrosis and oil-phase α-linolenic acid for anti-inflammatory and plaque-stabilizing effects. It constructs a three-tiered comprehensive application system covering daily health maintenance, organ damage repair, early-stage fibrosis, and blocking of high-risk vascular lesions. This system can be extended to adapt to various chronic disease herbal compositions and industrial applications in multiple dosage forms such as tea, granules, and capsules. This invention is highly original, has a closed-loop pharmacological logic, and is highly applicable, filling the gap in existing technologies for fixed dual-core formulations and water-oil dual-phase comprehensive conditioning frameworks. It possesses extremely high scientific research value, intellectual property barriers, and industrial application value.

This invention belongs to the field of plant genetic engineering technology, specifically relating to the application of a tea plant transcription factor gene and its expression promoter in regulating anthocyanin content in plants. The tea plant transcription factor gene is... CsMYB44 Its nucleotide sequence is shown in SEQ ID NO.1. The regulation is to reduce the anthocyanin content in plants. This invention discovered a tea tree transcription factor. CsMYB44 It can inhibit the accumulation of anthocyanins in plant leaves, and has good application prospects in improving tea varieties where the quality of tea leaves is reduced due to purple discoloration, as well as in regulating plant leaf color.

This invention discloses a tea plant CsFEZ gene, its encoded protein, and its applications, specifically relating to the field of tea plant breeding technology. The CDS sequence of the tea plant CsFEZ gene is shown in SEQ ID No: 1, and the amino acid sequence of its encoded protein is shown in SEQ ID No: 2. By controlling the expression of the CsFEZ gene and its encoded protein in tea and rice somatic embryos, the occurrence of somatic embryos in tea and rice can be effectively regulated to obtain somatic embryos with high regeneration efficiency. This can provide a new solution for the genetic transformation of varieties with low regeneration efficiency. Furthermore, the CsFEZ gene can serve as an important molecular mechanism in the process of plant somatic embryo regeneration, providing a foundation for research and application in plant gene synthesis and customized plant modification.

Pharmaceutical or cosmetic composition intended to improve the appearance of cellulite and reduce varicose veins, characterized in that it comprises an association of theanine and polysaccharides extracted from Camellia sinensis, and at least 2 metabolites produced by a Bacillus subtilis bacterium such as Bacillus subtilis Gozen GZN-08.

This invention discloses an innovative processing technology for scenting tea flowers into black tea. The technology first carefully selects high-quality tea flowers from specific tea varieties to ensure their freshness and quality. Then, the tea flowers undergo meticulous cleaning and drying to remove impurities. Next, the pre-treated tea flowers are mixed with selected tea leaves in a precise ratio and placed in a specific environment for scenting. During the scenting process, key parameters such as temperature, humidity, and time are strictly controlled to fully integrate the aroma and flavor of the tea flowers and tea leaves. After scenting, a carefully designed baking and drying process ensures the tea reaches the appropriate moisture content, thereby guaranteeing its taste and quality. This invention's processing technology is unique and scientific, producing a tea flower-scented black tea with a unique aroma and rich taste. It not only meets consumers' demand for high-quality scented tea but also brings new development opportunities to the scented tea industry, possessing significant economic and social benefits and a broad market prospect.

Disclosed in the present invention is a use of a 2-cyano-3-aminoacrylate compound in control of fungal diseases caused by Pestalotiopsis-like fungi. The compound exhibits generally ultrahigh ex-vivo antifungal activity against Pestalotiopsis-like fungi, including Pestalotiopsis, Neopestalotiopsis, and Pseudopestalotiopsis. Phenamacril exhibits a median effective concentration (EC50) ranging from 0.1455 to 0.6025 μg / mL, a mean value of 0.2800 μg / mL and a standard deviation of 0.095 μg / mL against 56 strains of Pestalotiopsis-like pathogenic fungi isolated from Camellia sinensis, Prunus persica, and Vitis vinifera. The Phenamacril suspension formulation spray treatment scheme for Camellia sinensis provided by the present invention can significantly increase tea yield, and provide a basis for controlling diseases caused by Pestalotiopsis, Neopestalotiopsis, and Pseudopestalotiopsis pathogens in tea plantations as well as for monitoring fungicide resistance.

An anti-allergic product comprising a plant extract in an amount of at least 0.01% w / w, wherein the plant extract is selected from the group consisting of Camellia sinensis leaf extract, Melaleuca alternifolia leaf oil, and mixtures thereof, an amphiphilic preservative, and a glycolipid. The eyelid cleansing composition is configured to reduce redness, discomfort, and irritation in and around the eyelids.

This invention discloses the CsGSTU8 gene of tea plant and its application in alleviating and degrading glyphosate toxicity, relating to the field of genetic engineering technology. The nucleotide sequence of the CsGSTU8 gene is shown in SEQ ID NO.1 of the sequence listing. The amino acid sequence of the protein encoded by the CsGSTU8 gene is shown in SEQ ID NO.2 of the sequence listing. CsGSTU8 is upregulated in both roots and leaves in response to glyphosate stress; CsGSTU8 is specifically highly expressed in tea plant roots. The pCAMBIA1305 plasmid (containing a GFP tag) constructed from this gene, after transformation with Agrobacterium and infection of tobacco leaves, was observed to be localized in the nucleus and cytoplasm. The CsGSTU8 protein is expressed in prokaryotes, and its enzyme activity is strongest at pH 8.0. Transforming the pYES2 plasmid constructed from this gene into the glyphosate-sensitive strain BY4741 restored the growth ability of the yeast mutant on high-concentration glyphosate medium. The cloning of this gene elucidates its role in tea plant resistance to glyphosate stress, providing data support for research on the molecular mechanisms of tea plant resistance to glyphosate stress.

This invention discloses a tea tree CsMYB82 Genes and their applications belong to the field of genetic engineering technology. Tea tree CsMYB82 The nucleotide sequence of the gene is shown in SEQ ID NO.1 of the sequence listing; the amino acid sequence encoding the protein is shown in SEQ ID NO.2. Studies have shown that... CsMYB82 The gene expression pattern is negatively correlated with the distribution of epidermal hairs (tea trichomes) in tea plants. Overexpression of this gene in plants can inhibit epidermal hair formation; inhibition can be achieved through antisense oligonucleotide technology. CsMYB82 CsMYB82 The expression of this gene can relieve its inhibition of key downstream factors, thereby promoting the development of epidermal hairs or maintaining the density of tea hairs. Cloning this gene is not only helpful in elucidating the negative regulatory mechanism of epidermal hair development in tea trees, but also provides a theoretical basis and genetic resources for the improvement of epidermal hair traits and the breeding of new varieties in tea trees.

The application discloses a preparation method and product of a makeup cushion powder capable of preventing makeup from being smudged, and the preparation process comprises a pre-activation step of 0.3-1.5 parts of quaternary ammonium salt-bentonite and 0.05-0.5 parts of tea (CAMELLIA SINENSIS) leaf extract, and 3-8 parts of trimethylsiloxy silicate is added in an oil phase preparation step. Through the synergistic effect of the three, the film forming mechanism of the makeup cushion powder system is completely different. By regulating the rheological properties of the system, a composite film layer with flexibility, rigidity and high adhesion is constructed, and a very strong anti-smudging effect is realized under the premise of light and thin skin. The application provides a new makeup cushion powder product and a premix oil gel for producing the same, and provides a new path for the production of the makeup cushion powder product.

The utility model discloses a kind of sieve flower devices for camellia sinensis production, comprising: U-shaped support seat, its bottom both sides are fixedly connected with two T-shaped seats, four T-shaped seats are used to support entire device;Screen frame, its top and left side are all set as opening, the bottom inner wall is set as inclined plane, the left side of screen frame is rotatably installed with the baffle matched with the opening of its left side, baffle is used to shield the left side opening of screen frame;Screen, it is two, and is embedded and fixed on the bottom inner wall of screen frame, screen is used to carry out screening operation to camellia sinensis.The utility model is set through a series of structures, can carry out screening operation to camellia sinensis, and after screening is completed, it is discharged by the way of blowing non-contact, can effectively the risk of camellia sinensis damage, and can directly carry out backblowing and clean impurities to screen after discharging, personnel does not need subsequent separate split cleaning, convenient personnel operation, to improve work efficiency.

The application discloses a kind of quick propagation methods of safflower oil tea stem segments in vitro, and relates to the field of in vitro quick propagation technology, the present application uses excellent safflower oil tea, and the semi-lignified stem segment of current year is collected as explant in mid-April-early May, and establishes in vitro efficient breeding system.The present application finds that Hyponex is used as the basic culture medium of safflower oil tea in vitro culture, and is added with growth regulator 6-BA and IBA, so that cluster shoots can be quickly obtained, and after multiple subcultures, callus induced at the base of single bud can be differentiated to form cluster adventitious bud cluster, and after periodic culture, rootless plants are developed.The rootless plants are immersed in rooting agent after rooting pretreatment, are cultivated into seedling bags to generate root system, and a seedling raising method for obtaining complete regenerated plants is provided.The method is a kind of efficient and stable quick propagation technology and can be applied to seedling raising production.

The invention relates to a pharmaceutical composition comprising a combination of bioactives including theanine and polysaccharides extracted from Camellia sinensis, and at least two metabolites typically produced by the bacterium Bacillus subtilis, preferably Bacillus subtilis Gozen GZN-08 isolated from Camellia sinensis. The invention also relates to the pharmaceutical composition for its use in modulating the pulmonary microbiota while modulating the intensity of the immune system response by restoring normal white blood cell counts and increasing antibody production while reducing the production of pro-inflammatory cytokines.

The application discloses a tea tree CsGSII-1b gene and application thereof in improving plant cold tolerance, wherein the tea tree CsGSII-1b gene is a tea tree glutamine synthetase gene, and the nucleotide sequence of the tea tree CsGSII-1b gene is shown in a sequence table SEQ ID NO.1. The tea tree CsGSII-1b gene in vivo expression inhibition can improve the low-temperature tolerance of plants. The cloning of the CsGSII-1b gene is not only beneficial to the exploration of the role of glutamine synthetase in the process of the tea tree response to cold stress, but also contributes to the genetic improvement process aiming at enhancing the cold tolerance of the tea tree, promotes the sustainable development of the tea industry, and has important application value.