122 results about "Camellia sinensis" patented technology

The invention relates to the technical field of plant genetic engineering, in particular to a transcription factor CsKNAT1 and application thereof in regulation and control of development of tea trees. The nucleotide sequence of the gene is as shown in SEQ ID NO: 1, and the sequence of protein coded by the gene is as shown in SEQ ID NO: 2. Research finds that overexpression of the CsKNAT1 gene can influence the form of plant leaves: the edges of the leaves are promoted to have notches (asymmetric leaves are formed, resulting in increase of sawteeth at the edges of the leaves); the formation of compound leaves can be promoted; leaves on plant stems can grow in a rosette form, and secondary stems are bolted in the middles of rosette leaves; the research also finds that the overexpression of the CsKNAT1 gene can obviously influence the flowering phase and the flower form of the plant; the delay of the flowering phase is promoted; overexpression of the CsKNAT1 gene can further influence development of floral organs and formation of siliques, part of arabidopsis plants have phenotypes that corolla becomes small, stamens are exposed, petals gradually extend and are zigzag in a wavy shape, the length of mature siliques is remarkably shortened by 20%-36.1%, and the phenomenon that the fruits are aborted occurs.

The present application relates to the field of compound medicine and female private part nursing technology, and particularly relates to a compound traditional Chinese medicine composition, a preparation method and application thereof.The compound traditional Chinese medicine composition is prepared from raw medicinal materials including the following components by weight: 8-10 parts of extractum of macaranga denticulata, 8-10 parts of extractum of calycopteris floribunda, 7-9 parts of extractum of panax notoginseng, 7-9 parts of extractum of saffron, 9-11 parts of extractum of camellia sinensis, and 7-9 parts of extractum of ganoderma lucidum.The compound traditional Chinese medicine composition has antibacterial and firming effects on female private parts, and has obvious performance advantages when applied to female private part nursing products such as sanitary napkins.

The invention provides a camellia oleifera seedling drought degree classification method and system based on multi-modal data, and belongs to the technical field of plant detection, the scheme is based on a ConvNeXt architecture, a graph convolutional network (GCN) and a dynamic fusion gate mechanism are combined, image data and root system data of camellia oleifera seedlings are effectively fused, fusion extraction of multi-modal data features is realized, and the method and the system have good application prospects. The classification accuracy of the drought degree of the camellia oleifera seedlings is effectively improved; according to the scheme, the spatial topology modeling capability of a leaf image is enhanced by introducing a graph convolutional network (GCN), the weights of the image and physiological features are adaptively adjusted in combination with a dynamic fusion gate, the weights of the image and root system features are adaptively adjusted through the dynamic fusion gate, the model can accurately capture complementary information among different modals, and the modeling efficiency is improved. And the accuracy of drought stress classification is remarkably improved.

The present application belongs to the field of plant genetic engineering and biotechnology, and particularly relates to a method for creating a dwarf material of Brassica napus by using CRISPR / Cas9 gene editing technology and application. The method edits the Brassica napus BnaA06G0083400WE gene by using CRISPR / Cas9 gene editing technology to obtain a dwarf material of Brassica napus; the nucleotide sequence of the coding gene mutant of the Brassica napus dwarf material is SEQ ID NO. 1. The present application edits the Brassica napus BnaA06G0083400WE gene by using CRISPR / Cas9 gene editing technology to obtain a dwarf Brassica napus, which provides valuable genetic resources and germplasm resources for Brassica napus breeding; the method has strong characteristics and is easy to obtain, is an effective way to realize the improvement of target traits and cultivate new materials, and can be applied to Camellia sinensis breeding.

The present application relates to a kind of whitening anti-aging composition suitable for sensitive skin and its application, belong to the technical field of cosmetics.The composition includes Psoralea corylifolia phenol, hydrolyzed royal jelly protein, radix notoginseng extract and camellia sinensis var. assamica flower extract as active ingredient.Psoralea corylifolia phenol has the effects of anti-aging, antioxidant and anti-inflammatory, etc.;Hydrolyzed royal jelly protein can help to relieve skin discomfort, moisturizing, enhance skin barrier function, etc.;Camellia sinensis var. assamica flower extract has the effects of anti-photoaging, whitening and anti-inflammatory soothing, while radix notoginseng extract has the effects of anti-inflammatory, antioxidant and anti-aging, etc.The composition of the present application can synergistically enhance the effects of anti-inflammatory, whitening and anti-aging by reasonable allocation of the above components, and the composition contains natural components, is mild and non-irritating, suitable for sensitive skin, and can be applied to various cosmetics.

The invention relates to a method for improving the survival rate of acclimatization and transplantation of camellia tissue culture seedlings. In the acclimatization process, the tissue culture seedlings are disinfected by a ferrous sulfate disinfectant and then are transplanted. The method successfully solves the problem of huge economic loss caused by discarding polluted camellia tissue culture seedlings which are difficult to breed or extremely precious, can realize normal growth of the tissue culture seedlings under the condition of bacterial colony pollution, and greatly improves the survival rate of the tissue culture seedlings after acclimatization and transplantation.

The invention discloses a method for realizing efficient in-vitro regeneration of tea tree cotyledons through a somatic embryo indirect generation way, and belongs to the technical field of plant tissue culture. The tea tree somatic embryo indirect generation system is successfully constructed by screening culture conditions, a culture medium formula and other methods, and a foundation is laid for construction of a tea tree genetic transformation system and industrialized seedling production; according to the method, immature cotyledons of tea trees are used as explants, the cotyledons are induced to generate calluses, then the calluses are proliferated and differentiated to generate somatic embryos, and the somatic embryos are further differentiated to form complete plants, so that efficient and synchronous propagation of tea tree seedlings is realized, and technical support is provided for high-quality variety propagation and new variety breeding of tea trees; the problems of low propagation coefficient, long growth cycle, serious descendant degeneration and the like in traditional seed propagation of tea trees are solved, and the technical bottlenecks of serious explant browning, high bacterial contamination rate, poor plant growth synchronism and the like in other tissue culture technologies are solved.

The application provides a self-made bag sealing device for camellia sinensis production and processing, and relates to the technical field of bag sealing equipment. The bag sealing device comprises bag sealing heating parts, two bag sealing heating parts are arranged, and the structures of the two bag sealing heating parts are the same. The two bag sealing heating parts are respectively arranged on a bag sealing machine. A bag sealing conveying part is arranged on the bag sealing heating part. The bag sealing conveying part is used for increasing the sealing area. A vibration auxiliary part is arranged at the bottom of the bag sealing heating part. The vibration auxiliary part is used for vibrating and flattening tea flowers. A pressure protection part is arranged on the bag sealing heating part. The pressure protection part is matched with the slidable bag sealing mounting shell. The bag sealing device can effectively avoid the increase of the breakage rate caused by the inconsistent bulk density of tea flowers after filling and the different volumes. The bag sealing device can automatically adapt to the volume of tea flowers for sealing and protection, and can automatically detect the sealing quality.

This invention relates to a method for obtaining aqueous extracts of tea leaves (Camellia sinensis), particularly fresh tea leaves, Pu-erh tea, matcha, or green tea. The invention also relates to tea extracts rich in small RNAs, sugars, phenolic compounds, organic acids, catechins, and theanine, as well as cosmetic compositions comprising such extracts and their cosmetic uses for the care of the skin, scalp, and appendages, particularly for protecting the skin from external aggressors and oxidation, combating signs of skin aging, enhancing photoprotection, brightening the skin, and improving skin hydration.

The invention discloses a tea tree CsPR10-5 gene and application thereof in enhancing anthracnose resistance of a tea tree, and belongs to the technical field of gene engineering. The nucleotide sequence of the CDS region of the gene is as shown in SEQ ID NO. 1. The invention further provides an application in regulating the anthracnose sensitivity of the tea tree, an anthracnose-sensitive tea tree model and an anthracnose-resistant tobacco model. The tea tree CsPR10-5 gene has the beneficial effects that the effect of the tea tree CsPR10-5 gene in regulation and control of tea tree anthracnose infection is provided, the tea tree CsPR10-5 gene has different expression quantities at different time points of anthracnose infected tea trees and can respond to germ infection at different time points, so that the biological function of mediating the anthracnose resistance of the tea trees is verified, and a key gene resource is provided for disease-resistant breeding of the tea trees.

The invention belongs to the technical field of tea tree cultivation, and particularly relates to a tea tree cultivation method for improving summer tea leaf quality and tea leaves. The technical problem to be solved by the invention is that the quality of tea trees is reduced in summer. According to the technical scheme, the method comprises the steps that after spring tea production is finished, tea tree canopies are pruned; building a shading shed above the trimmed tea trees, and covering the trimmed tea trees with a shading net; a spectrum selective light film is laid above the sunshade net in the tea row direction, so that a stable shading light environment is provided for tea trees before summer tea is picked. The tea is prepared from one bud and two leaves of the tea tree cultivated by the method, the main purpose of the invention is to provide a new choice for the tea tree cultivation method for improving the quality of the summer tea and the tea, and a new technical path is provided for directional quality improvement and high-value utilization of the summer tea.

The utility model provides an oil tea tree pruning and shaping device which comprises a main body mechanism, the main body mechanism comprises a protection assembly, a first pruning assembly arranged in the protection assembly and a second pruning assembly arranged at the bottom of the first pruning assembly, and the protection assembly comprises a protection cover arranged on the outer wall of the first pruning assembly. The first trimming assembly comprises a protective cover, a protective sleeve arranged at one end of the protective cover and a protective groove formed in the protective cover, and the first trimming assembly comprises a connecting piece arranged in the protective sleeve, an open hole formed in the connecting piece and clamping rods arranged on the two sides of the connecting piece; when the pruning device is not used, the protection cover and the protection sleeve are connected to the outer walls of the first pruning assembly and the second pruning assembly in a sleeving mode to protect the first pruning assembly and the second pruning assembly, personnel are prevented from being scratched, meanwhile, the cutting edge part is protected, and when the cutting edge is damaged, the fixing piece at the top of the butt joint sleeve is unscrewed, the butt joint sleeve is taken out of the outer wall of the connecting piece, and then the cutting edge is integrally replaced.

The present application belongs to the technical field of cosmetic compositions, and particularly relates to a composition with a repairing effect on dyed and permed hair and application thereof. The composition comprises willow herb / leaf / stem extract, lentil fruit extract, blueberry fruit extract and camellia sinensis var. assamica flower extract, and the weight ratio of the willow herb / leaf / stem extract, the lentil fruit extract, the blueberry fruit extract and the camellia sinensis var. assamica flower extract is (8-32):(7-35):(15-50):(10-100). The composition realizes a synergistic repairing effect on damaged hair. On the one hand, the composition can effectively penetrate into the inside of hair, supplement lost nutrients, and enhance the toughness and elasticity of hair; at the same time, the composition can smooth up the raised scales, significantly reduce the resistance during combing, and make the hair smooth and easy to comb; on the other hand, the composition can effectively repair the scales, and make the hair present a healthy and shiny state.

Disclosed are use of the Camellia sinensis (L.) O. Kuntz CsMYB1 gene in improving disease resistance of a plant and a method for improving disease resistance of a plant. The CsMYB1 gene is a transcription factor gene of the MYB family. CsMYB1 is constructed into a plant expression vector, and the vector is transferred into Nicotiana benthamiana. A transgenic tobacco plant is obtained by means of stable genetic transformation, and the plant exhibits relatively strong fungal inhibitory activity against inoculated Botrytis cinerea hyphae. Transient silencing of the CsMYB1 gene of Camellia sinensis (L.) O. Kuntz leaves using a technology of antisense oligonucleotides (AsODNs) significantly reduces the disease resistance of the plant. Therefore, the CsMYB1 gene has the effect of improving the disease resistance of a plant and can be used as a disease-resistant gene. Introducing the gene into plants such as tobacco, Camellia sinensis (L.) O. Kuntz, or vegetables can improve the disease resistance of these plants. The present invention has broad market application prospects.

The application discloses a kind of composition of adjusting overall metabolic balance, inhibiting cancer cell growth, antitumor, raw material includes ganoderma lucidum, crocodile peptide, astragalus, polypodium pinnatum, ginseng, turmeric, clove, poria cocos, camellia sinensis, rich glutathione yeast, cordyceps militaris, gizzard gold, peach kernel, compound probiotics, brassica campestris l.var.rapa.The application fuses modern medical scientific concept and traditional Chinese medicine "blood stasis and stagnation and cause mass" theory, raw material synergistic effect, by improving blood oxygen content, anti-inflammatory, improve metabolic function, blood circulation and other ways, realize protection mitochondria from damage, anti-mutation;And through the way such as supplementing compound probiotics, regulate intestinal flora, enhance immunity, finally realize soft and hard knot, adjust overall metabolic balance, inhibit cancer cell growth, antitumor, relieve tumor treatment side effect, application prospect is wide.

The application provides a method for regulating synthesis of catechin in tea tree based on CsAHL20 gene and application, and belongs to the field of plant molecular biological technology. The tea tree gene CsAHL20 screened in the application can combine with the CsANR gene promoter of a key enzyme for synthesis of catechin, and inhibit accumulation of catechin content in tea tree; through verification, silencing of the gene by using a virus-induced gene silencing technology can increase catechin content in tea tree, and overexpression of the gene by using an agrobacterium-mediated transient transformation technology can reduce catechin content in tea tree, so that the CsAHL20 gene can be used for regulating synthesis of catechin in tea tree.

The invention discloses a camellia oleifera close planting vigorous afforestation sapling cold-proof and anti-freezing protection device, and relates to the technical field of sapling protection, the camellia oleifera close planting vigorous afforestation sapling cold-proof and anti-freezing protection device comprises a stable base, a supporting protection plate and protection baffles, the supporting protection plate is arranged in the protection baffles, and the protection baffles comprise the first protection baffle and the second protection baffle; the number of the stable bases is two, and the number of the supporting protection plates is four. According to the cold-proof and anti-freezing protection device for the camellia oleifera close planting vigorous afforestation young trees, the combined design of the stable base, the double-layer protection baffles and the inner-layer supporting protection plates is adopted, conical stable piles at the bottom of the stable base can penetrate into soil, and the anti-dumping capacity of the device in complex terrains such as mountainous regions and sloping fields and in the strong wind environment is greatly improved; the first protective baffle and the second protective baffle are matched in an inserted mode through the limiting strips and the limiting grooves, the bottoms of the first protective baffle and the second protective baffle and the stable base form multiple fixation through the clamping blocks, the L-shaped connecting blocks and the inserting blocks, and a complete and firm outer layer protective barrier is formed after splicing.

The invention relates to the technical field of bioflavonoid extraction, in particular to a camellia nitidissima flavone extraction and purification preparation method, which comprises the following steps: weighing dried camellia nitidissima, and crushing into powder; adding an ethanol solution, stirring and dispersing, and sealing and infiltrating the mixed solution after stirring and dispersing; after infiltrating, carrying out microwave extraction and filtration on the mixed solution, and carrying out vacuum concentration on the filtrate to obtain a camellia nitidissima flavone crude extract; adding an ethanol solution into the camellia nitidissima flavone crude extracting solution, mixing and filtering, collecting filtrate, and performing centrifugal treatment to obtain an alcohol precipitation concentrated solution; and diluting the alcohol precipitation concentrated solution to 5-8 times of the volume of the original alcohol precipitation concentrated solution to obtain a diluent, extracting the diluent with an extracting agent for 4-6 times, combining the extract liquids, carrying out reduced pressure distillation on the extract liquids until the extract liquids are dried, taking out the dried solid, and crushing to obtain the camellia nitidissima flavone refined extract. The camellia nitidissima flavone provided by the invention is excellent in antioxidant activity, and the purity of the camellia nitidissima flavone is improved.

This invention discloses the tea plant miRNA miR408-p5 and its application and method in improving plant disease resistance. By constructing a silencing expression vector for miR408-p5 and transferring it into tobacco, transgenic tobacco plants were obtained through stable genetic transformation, exhibiting strong fungal inhibitory activity against *Botrytis cinerea*. Conversely, by constructing an overexpression vector for miR408-p5 and transferring it into tobacco, transgenic tobacco plants were obtained through stable genetic transformation, showing significantly weakened resistance to *Botrytis cinerea*. Therefore, silencing miR408-p5 expression can improve plant disease resistance, and introducing the miR408-p5 silencing expression vector into plants such as tobacco, tea, or vegetables to enhance plant disease resistance has broad market application prospects.

The utility model discloses a land plowing machine for camellia planting, which relates to the technical field of land plowing machines and comprises a machine body, a mud guard is arranged on the right side of the machine body, an operating rod is arranged above the mud guard, and two connecting rods are movably sleeved on one side, opposite to the machine body, of the operating rod. The two threaded rings are rotated leftwards to be separated from the control rod, limiting is opened, the control rod can stretch out and draw back on the two connecting rods at the moment, after the control rod is adjusted to the needed length, the two threaded rings are rotated again to be reset, and the control rod and the two connecting rods are in a limiting and fixing state at the moment. The arm lengths of operators with different heights and body types are different, and the adjustable operating rod can enable each operator to find a suitable operating position, so that the arms naturally extend, fatigue and muscle tension caused by long-time operation are reduced, and the working efficiency is improved.

The present invention is related to a skin care composition comprising (1) a physiologically acceptable medium and (2) a synergistic composition consisting of: a blend of a Nelumbo nucifera (Sacred Lotus) serum fraction and a Camellia sinensis (Tea) serum fraction in a respective weight ratio of 75:25; or a blend of a Nelumbo nucifera (Sacred Lotus) serum fraction and a Chamomilla recutita (German chamomile) serum fraction in a respective weight ratio of 75:25. The invention also relates to a method for improving skin appearance associated with skin aging comprising applying compositions thereof.

The invention discloses an integrated golden camellia sapling cultivating and transplanting device, and relates to the technical field of sapling cultivating. The device specifically comprises a chassis and a track driving mechanism arranged at the bottom of the chassis, a vertical frame is fixed to the outer wall of the top of the chassis through bolts, a cultivation mechanism used for cultivating golden camellia seedlings is arranged on the inner side of the vertical frame, and a soil breaking mechanism used for breaking soil is arranged at one end of the chassis; the top of one end of the base plate is provided with an automatic material taking and placing mechanism used for taking out golden camellia seedlings cultivated in the cultivation mechanism and placing the golden camellia seedlings into soil. On the basis that the multiple cultivation assemblies are arranged and adopt centralized cultivation, the two chain transmission assemblies are used for driving the cultivation assemblies to move, so that the multiple cultivation assemblies can periodically or continuously move, camellia nitidissima seedlings in the different cultivation assemblies can sequentially pass through an illumination area, and the cultivation efficiency is improved. Therefore, the golden camellia seedlings in all the cultivation assemblies can receive light reliably, the problem of light shielding is solved, and the cultivation quality is guaranteed.

The utility model discloses a kind of temperature control rocking machine for Meiren tea making green process, specifically related to tea making equipment field.The temperature control rocking machine for Meiren tea making green process provided by the utility model, including rack, rocking barrel, temperature control device, variable frequency driving device, the rocking barrel includes stainless steel cylinder and bamboo splint cylinder, the bamboo splint cylinder is set in the stainless steel cylinder, the temperature control device is set in the stainless steel cylinder is used to adjust the temperature in the stainless steel cylinder to simulate natural day and night temperature difference, the variable frequency driving device is set on the rack, the variable frequency driving device is connected with the bamboo splint cylinder and is used to drive the bamboo splint cylinder variable frequency rotation to dynamically adjust rocking strength.

The application discloses a polypeptide-wrapped anti-wrinkle composition containing camellia sinensis leaf extract and application thereof, which comprises component A, component B and component C; the component A comprises palmitoyl tripeptide-5, palmitoyl tetrapeptide-7, polyol A and caprylic / capric triglyceride; the component B comprises PPG-13-decyltetradecin polyether-24 and polyol B; and the component C comprises water and camellia sinensis leaf extract. The application firstly solves the solubility problem of palmitoyl peptides, and secondly solves the compatibility problem of camellia sinensis leaf extract and palmitoyl peptides by low-energy emulsification technology, so that a transparent flowing liquid is obtained, which is more stable, safer, more effective, more convenient to apply, can promote collagen production and inhibit the activity of elastase, and better achieves the effects of improving wrinkles and delaying aging.

The invention discloses a tea tree pruning efficient degradation microbial combined bacterium, a microbial agent and application of the microbial agent, the tea tree pruning efficient degradation microbial combined bacterium comprises bacillus JKB11 and paenibacillus polymyxa JKB16, and the JKB11 and JKB16 combination in the tea tree pruning efficient degradation microbial combined bacterium can effectively deal with degradation of tea tree pruning; although the living enzymes of cellulose degrading enzyme (CMCase) and lignin degrading enzyme (lignin peroxidase (LiP) and manganese peroxidase (MnP)) are similar to those of JKB16, the three-element content and infrared spectrum indexes of the degradation product prove that the tea tree pruning degradation effect of the combination of JKB11and JKB16 is better.

The invention discloses a tea tree MYB transcription factor CsMYB330 gene and application, the nucleotide sequence of the MYB transcription factor CsMYB330 gene is shown as SEQ ID NO: 1, the expression of the gene participates in regulating the flavonol content in plants, and the instantaneous silencing of the CsMYB330 gene in the tea tree can significantly reduce the flavonol content in the tea tree by 24.5% and reduce the bitter taste of the finished tea.

The invention provides a marker for identifying a Yinghong No.9 tea tree. The marker is composed of two specific DNA (Deoxyribose Nucleic Acid) sequences. The invention also provides a method for identifying the Yinghong No.9 tea tree by using the marker, total DNA (deoxyribonucleic acid) of a sample is extracted, then high-throughput sequencing is performed, sequencing reads are compared to the marker, and if the marker can be completely covered and a consistent sequence which is completely the same as the sequence of the marker is generated, the species of the sample to be detected is judged to be the Yinghong No.9 tea tree.

The present disclosure provides a process for obtaining a concentrated extracellular vesicles suspension. The method can include fermenting tea (camellia sinensis) with a combination of at least one yeast and bacterium to obtain a fermentation product comprising a liquid material; filtering the fermentation product through a filtering device to give a permeate; filtering the permeate through a second filtering device to give a suspension comprising extracellular vesicles; and recovering the suspension of extracellular vesicles.

The present disclosure discloses a composition for anti-graying hair comprising Aloe barbadensis Leaf Extract, Camellia sinensis leaf extract, Rosmarinus officinalis Leaf Oil, Hamamelis virginiana (Witch Hazel), Neopicrorhiza scrophulariiflora root extract, Tetrasodium ethylenediaminetetraacetic acid (EDTA), Acrylates / C 10-30 Alkyl Acrylate Crosspolymer, Panthenol, Polyalkylene Glycol Mono Butyl Ether, Ethylhexylglycerin, Phenoxyethanol, Sodium Benzoate, Potassium Sorbate, Propylene Glycol, Biotin, Hyaluronic Acid, Saccharide Isomerate, Alpha Glucosyl Hesperidin, Dimethyl Isosorbide, Mandarin peel paste, N-Acetyl L-Tyrosine, Pentylene Glycol, Gluconolactone, Propanediol, and water is added to the composition until a desired volume is achieved. The method for preparing the composition for anti-gray hair is also provided.

The utility model discloses a water replenishing device for camellia oleifera planting. The water replenishing device comprises an outer box and a buried pipe indirectly connected with the outer box. The outer boxes can be connected end to end or vertically connected, and the buried pipes are hollow and are provided with a plurality of water seepage holes in a communicating manner; the fertilizer storage bin is a net-shaped cuboid, an opening is formed in the upper end of the fertilizer storage bin, and the fertilizer storage bin is located in the box body and can be taken out; the upper end of the U-shaped pipe extends into the outer box, and the lower end is communicated with the buried pipe; the middle of the U-shaped pipe is disconnected and detachably connected with a turbulent flow pipe, and a spiral plate is arranged in the turbulent flow pipe. Water is directly guided into the root system of a plant through the buried pipe which can be inserted into the ground, the water can be freely spliced and used according to the area of water needing to be supplemented, the water supplementing effect is good, and evaporation and ineffective water supplementing are reduced; solid fertilizer is put into the fertilizer storage bin of the outer box when needed, liquid fertilizer can be guided into the root system of the plant through flushing dissolution, the fertilizing effect is good, and the practicability is high.