515 results about "Genetic resources" patented technology

The invention belongs to the field of plant genetic engineering, and discloses application of a vitis amurensis cold-resistant gene VaWRKY65 in plant cold-resistant genetic improvement, the VaWRKY65 gene is a transcription factor separated and cloned from extremely cold-resistant vitis amurensis, and the sequence of the VaWRKY65 gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the vitis amurensis cold-resistant gene VaWRKY65. The gene is used for constructing an overexpression and interference vector, the overexpression and interference vector is respectively introduced into arabidopsis thaliana and vitis amurensis through agrobacterium tumefaciens-mediated genetic transformation, and biological function verification of the obtained transgenic plant shows that the cloned VaWRKY65 gene has the function of improving the cold resistance of the plant. The discovery of the gene provides a new gene resource for plant stress resistance molecular design breeding and provides a new genetic resource for implementation of green agriculture and water-saving agriculture, and development and utilization of the genetic resource are beneficial to reduction of agricultural production cost and realization of environmental friendliness.

The invention discloses a sandalwood SaERF33 protein and / or a SaERF33 gene and application of the sandalwood SaERF33 protein and / or the SaERF33 gene in promoting synthesis of sandalwood essential oil. The amino acid sequence of the sandalwood SaERF33 protein is as shown in SEQ ID NO. 2. According to the invention, the SaERF33 gene is obtained from sandalwood for the first time, the synthesis of sandalwood essential oil can be obviously promoted by overexpressing the SaERF33 gene in sandalwood, and the normal growth of plants is not influenced. The invention provides a new genetic resource for cultivating a new sandalwood variety with high essential oil synthesis efficiency, and has important popularization and application values in the aspects of sandalwood variety cultivation and sandalwood essential oil production.

The invention belongs to the field of molecular biological identification, and particularly relates to a specific SNP (Single Nucleotide Polymorphism) site combination for identifying a Wuzhishan pig variety and application. The specific SNP site combination for identifying the Wuzhishan pig variety comprises 77 SNP sites, and the physical positions of the SNP sites are determined by sequence comparison based on a pig reference genome Sscrofa11.1. The specific SNP site combination is screened based on a method of combining whole genome association analysis with selection signal analysis so as to ensure the accuracy of site selection. The selected specific SNP site combination can rapidly realize accurate identification of the Wuzhishan pig variety on the gene level, and has significant application value in the aspects of genetic resource accurate protection and variety utilization of Hainan Wuzhishan pigs.

The invention discloses a gene PtoMYB20 for regulating and controlling salt tolerance and drought resistance of poplars. A nucleotide sequence of the gene comprises a sequence as shown in SEQ ID NO. 1. The gene interference fragment is introduced into a plant to obtain a silent transgenic plant, under the conditions of salt stress, drought stress and salt-drought composite stress, the H2O2 and MDA contents of the silent transgenic plant are lower than those of a wild plant, and the SOD and POD activities of the silent transgenic plant are higher than those of the wild plant, so that the damage degree of the plant is effectively reduced; the method is of great significance in improving salt tolerance and drought resistance of the poplar and relieving damage of adversity stress to forestry yield. The invention also discloses a breeding method of the transgenic poplar with strong salt tolerance and drought resistance, provides a new poplar germplasm which not only improves the stress resistance of the poplar but also does not influence the wood yield, and lays a theoretical and gene resource foundation for the stress-resistant molecular breeding of the poplar.

The invention relates to the technical field of bioinformatics, in particular to a goat selective breeding method and system based on a genetic algorithm, and the method comprises the following steps: based on goat population genetic initialization data, extracting a character characteristic index and a genetic relationship parameter of each pairing combination in a population, analyzing genetic diversity, character matching degree and genetic relationship, and calculating the genetic diversity, character matching degree and genetic relationship; and screening matched individuals and pairing hereditary characteristics, and rearranging and recombining to obtain primary pairing data of goat breeding. According to the method, genetic diversity and character matching degree are analyzed, genetic diversity and excellent character inheritance are balanced, genetic resource utilization efficiency is improved, local optimal combinations are accurately screened, genetic drift risks are reduced, character stability is enhanced, a two-point crossing rule and global character dynamic analysis are combined, and global breeding layout is optimized; according to the method, short-term improvement and long-term stability are both considered, the genetic structure is optimized through Pareto rule screening and global state updating, and the sustainability and accuracy of a breeding scheme are improved.

The present invention discloses a gene, GmCDK8, that negatively regulates soybean resistance to Phytophthora spp., and its applications. The disclosed GmCDK8 knockout soybean plants exhibit enhanced resistance to the fungus. Specifically, the results of the soybean cultivar "Williams 82," which lacks GmCDK8, under Phytophthora stress, including root lesion length, leaf lesion area, Phytophthora accumulation, and infected necrotic area on leaves, indicate that knocking out GmCDK8 significantly improves plant resistance to Phytophthora spp. Genetic engineering of this gene for plant resistance to Phytophthora root rot not only provides an important genetic resource for plant molecular breeding but also offers an effective means for achieving high plant yields.

The invention relates to the technical field of gene engineering, in particular to a soybean disease-resistant RING-H2 type zinc finger protein gene GmRHF1 and application thereof.According to the technology, the GmRHF1 genes are cloned in soybean varieties Xu bean 14 and Shangshan black beans respectively, and then the expression quantity difference of the genes in different tissues and the expression quantity change trend of the genes under SMV treatment are analyzed through fluorescent quantitative PCR; meanwhile, the anti-SMV function of the GmRHF1 is verified in effective plant silencing and transgenic overexpression by adopting a virus-induced gene transient silencing technology and a soybean stable genetic transformation experiment. Finally, the interaction protein of the GmRHF1 is screened and verified through yeast cDNA library screening, yeast point verification and luciferase complementary test screening, and the biological significance of interaction of the GmRHF1 and the GmClpP6 on soybean SMV resistance is defined. In conclusion, the result of the invention can provide an effective gene resource for cultivating a new variety (line) of soybean with high SMV resistance.

The invention discloses a gene GmCDK8 for negative regulation and control of phytophthora sojae resistance and application of the gene GmCDK8. GmCDK8-knocked-out soybean plants disclosed by the invention enhance the resistance of soybeans to phytophthora sojae; the results of root scab length, leaf scab area, phytophthora accumulation amount, leaf infected and necrotic areas and the like of the soybean cultivar Williams 82 without the GmCDK8 under the stress of phytophthora show that the resistance of the plant to the phytophthora can be remarkably improved by knocking out the GmCDK8. Genetic engineering improvement of plant phytophthora root rot resistance is carried out through the gene, important gene resources are provided for plant molecular breeding, and an effective means is provided for high yield of plants.

The invention discloses application of an OsLOX1 gene in regulation and control of drought resistance and seed vigor of rice, and relates to the technical field of plant genetic engineering. According to the invention, the drought stress tolerance of the mutation line with the OsLOX1 knocked out through CRISPR / Cas9 is lower than that of a wild type line, and along with the increase of the content of hydrogen peroxide (H2O2) and malondialdehyde (MDA) and the reduction of the expression quantity of antioxidant enzyme related genes. In addition, it is found that compared with a wild type, the service life of rice seeds is shortened by knocking out OsLOX1, meanwhile, the content of H2O2 and MDA is increased, and the activity of antioxidant enzyme superoxide dismutase (SOD) and catalase (CAT) is reduced; the method can be used for breeding storage-resistant and drought-resistant rice varieties; important gene resources are provided for cultivating drought-resistant rice varieties and prolonging the service life of seeds.

The invention belongs to the field of plant genetic engineering, and discloses application of a PtNF-YB9 gene of trifoliate orange in drought-resistant genetic improvement of plants. The PtNF-YB9 gene is a transcription factor gene which is separated and cloned from Poncirus trifoliata, and the sequence of the PtNF-YB9 gene is as shown in SEQ ID NO. 1. The gene or the homologous gene thereof is knocked out or inhibited in a plant, the drought resistance of the plant is remarkably reduced, and the drought resistance of the plant is remarkably enhanced by overexpressing the gene or the homologous gene thereof. The development and utilization of the genetic resource are beneficial to reducing the agricultural production cost and realizing the green and environment-friendly agricultural goal.

The invention discloses application of an OsCPN10a gene in regulation and control of vigor, grain shape and drought tolerance of rice seeds, and belongs to the technical field of rice gene engineering. The invention particularly relates to the research of the OsCPN10a gene in the aspects of regulating and controlling the germination rate, the germination potential, the germination index, the grain length, the grain width, the thousand grain weight, the drought tolerance of the rice seeds and the like. Experimental results show that the grain shape of the OsCPN10a knockout mutant seed is obviously lengthened and widened, and the grain shape of the OsCPN10a overexpression seed is opposite; the yield of the Ocpn10a mutant is higher than that of a TNG67 plant; overexpression of the OsCPN10a has the potential of improving the drought resistance of the rice. The invention reveals that OsCPN10a affects genetic mechanisms such as rice seed vigor, grain length, grain width and storability, provides important gene resources and theoretical basis for genetic improvement of rice, and is beneficial to cultivation of a new rice variety with better grain shape and higher yield.

The invention belongs to the technical field of biological detection, and discloses an SNP (Single Nucleotide Polymorphism) site combination for rapidly identifying Chinese merino sheep. The invention provides the SNP locus combination for rapid identification of the Chinese merino sheep, and the combination consists of 10 SNP loci and has high specificity and relatively high prediction accuracy, so that variety identification of the Chinese merino sheep is realized. By integrating a molecular marker technology and bioinformatics analysis, the identification process is simplified, the identification efficiency and reliability are improved, and a solid foundation is laid for effective protection, scientific management and reasonable utilization of genetic resources of the Chinese merino sheep.

The invention discloses application of a rice OsbHLH083 gene in regulation and control of seed germination capability, and relates to the technical field of plant genetic engineering. The CDS sequence of the rice OsbHLH083 gene is as shown in SEQ ID NO. 2, and the amino acid sequence of the coded protein is as shown in SEQ ID NO. 3. The molecular biology function of the rice OsbHLH083 gene for regulating rice germination is studied, after the rice OsbHLH083 gene is edited through CRISPR / Cas9, compared with a wild type, the rice OsbHLH083 gene mutant rice seeds germinate slower, the rice OsbHLH083 gene and a protein coded by the rice OsbHLH083 gene can be used for regulating the phenotype of seed germination, and the rice OsbHLH083 gene mutant rice seeds germinate more slowly. And a gene resource is provided for the genetic improvement of rapid germination and seedling formation characters of the direct seeding rice seeds in the field.

The invention belongs to the technical field of agricultural biology, and particularly relates to a transcription factor StBSB with a regulation effect on steroid glycoalkaloid accumulation of potatoes and application of the transcription factor StBSB, the transcription factor StBSB is encoded by a potato gene Soltu.DM.05G001620, and the nucleotide sequence of the potato gene Soltu.DM.05G001620 is shown as SEQ ID NO.1. The invention further discloses a preparation method of the transcription factor StBSB. According to the application, a new insight is provided for understanding a transcriptional regulation mechanism of SGAs in solanaceae plants, and a key theoretical basis and genetic resources are provided for directionally improving the content of anti-nutritional compounds through genetic means.

The invention belongs to the technical field of agricultural biology, and particularly relates to a GmGAI7 gene and application of the GmGAI7 gene in soybeans. A jy425 dwarfing mutant is obtained by screening and identifying an EMS (Enhanced Mass Spectrometry) mutagenesis group of JY47 (JY47), then BSA (Bovine Serum Albumin) sequencing analysis and fine positioning are carried out on a filial generation of jy425 * JN18 to find and verify that SNP variation exists at a chr18: 3281001 site of a soybean genome, so that non-synonymous mutation (gTc / gAc) of amino acid occurs, and a molecular marker is developed aiming at the site by a KASP method; meanwhile, it is found that the soybeans are insensitive to gibberellin due to mutation (T-> A) of the GmGAI7 gene, and the plant height of the soybeans is remarkably reduced. Therefore, cognition on ideal plant types of soybeans is widened, important genetic resources are provided for molecular marker-assisted breeding, and the breeding process is more accurate, efficient and controllable.

The invention discloses an application of a tomato B3-337 transcription factor in improvement of tomato quality, a gene for coding a B3-337 transcription factor protein is named as a B3-337 gene, and the nucleotide sequence is shown as SEQ ID NO.1. The invention also discloses an application of the tomato B3-337 transcription factor in improvement of tomato quality. According to the application, a carrier pFM3100GFP-B3-337 is constructed, so that the B3-337 is proved to be positioned in a cell nucleus. A transcriptional activity detection result proves that B3-337 has transcriptional activation activity. RT-qPCR (real-time quantitative polymerase chain reaction) and GUS (glucuronidase) dyeing show that the transcription factor is expressed in pericarp, seed, placenta and center pillar of each fruit ripening stage. According to the invention, a B3-337 gene overexpression plant is obtained by constructing a B3-337 overexpression vector, and compared with a wild type, the tomato fruit overexpressed with the B3-337 gene has the advantages that the content of soluble solids brix is increased, the fruit becomes larger, and the weight becomes heavier. It is shown that the B3-337 transcription factor plays an important regulation role in positive regulation of tomato fruit quality. The invention lays a good gene genetic resource foundation for the cultivation of new varieties of high-quality tomatoes.

The invention belongs to the technical field of agricultural biology, and particularly relates to a sisal hemp Aux / IAA gene and application of protein of the sisal hemp Aux / IAA gene in plants. The sisal hemp Aux / IAA gene provided by the invention comprises a first SNP (Single Nucleotide Polymorphism) site or a second SNP site. Research finds that the Aux / IAA gene can increase the number of the vascular tissues of the plant, construct a plant model related to the vascular tissues and cultivate the multi-vascular tissue plant; the first SNP site or the second SNP site on the gene is significantly related to the number of vascular bundles of sisal hemp leaves, and can be used for screening and cultivating multi-vascular tissue plants, thereby providing a theoretical basis and gene resources for genetic breeding.

The invention relates to a wheat stem rot regulatory gene Tatrx-m, and an encoding protein, a recombinant vector and an application of the wheat stem rot regulatory gene Tatrx-m. The genomic sequence of the gene is SEQ ID NO.1, the CDS sequence of the gene is SEQ ID NO.2, and thioredoxin shown as SEQ ID NO.3 is encoded. The invention further provides a specific primer pair used for gene identification, a silent vector (Tatrx-m-VIGS) and an overexpression vector (LGY-OE3-Tatrx-m) are constructed, and the function of the primer pair is verified through a virus-induced gene silencing (VIGS) technology and agrobacterium-mediated transformation of wheat. Experiments show that silence of Tatrx-m results in significant reduction of wheat stem rot resistance, and overexpression of the gene can improve resistance. Based on hypha quantification, DAB staining, H2O2 content and cell death analysis, Tatrx-m is revealed to enhance wheat resistance by regulating active oxygen removal and cell wall strengthening pathways. The gene TaTrx-m has the positive regulation effect in wheat stem rot for the first time, can be applied to disease-resistant molecular marker development, gene editing and transgenic breeding, provides core genetic resources for disease-resistant variety breeding, and has theoretical and application values.

The invention belongs to the technical field of plant molecular biology, and discloses an application of a soybean GmSW10 gene in reducing soybean plant height, grain weight and grease content and / or increasing protein content. The CDS sequence of the soybean GmSW10 gene is as shown in SEQ ID NO: 1. Knockout of the soybean GmSW10 gene can directly influence the plant height, the grain weight, the oil content and the protein content of soybean seeds, after the soybean GmSW10 gene is knocked out, the weight of soybean seeds and hundred grains is remarkably reduced, the plant height is reduced, the protein content is increased, and the oil content is reduced, which indicates that the GmSW10 gene is a key gene for regulating and controlling the plant height, the grain weight, the oil content and the protein content of the soybean, and the GmSW10 gene can be used for preparing the soybean GmSW10 gene for regulating and controlling the plant height, the grain weight, the oil content and the protein content of the soybean. And a new genetic resource is provided for genetic improvement of soybeans.

The invention provides a local livestock and poultry variety identification method based on an SNP marker, and relates to the field of variety identification, and the method comprises the following steps: S1, genotype data acquisition, S2, genotype data quality control, S3, variety identification standard library establishment, and S4, bloodline content evaluation. According to the method, on the basis of a standardized molecular identification analysis process of genomic SNP marker bloodline content evaluation, genotype information of a core SNP site is analyzed, so that whole analysis process processing from original genotyping data to variety identity judgment is realized; the method can provide standardized, convenient and efficient molecular detection technical support for protection and utilization of genetic resources of local livestock and poultry in China, germplasm innovation and industrial development.

The invention belongs to the technical field of biology, and particularly relates to a TaGAT2-1B gene resistant to wheat stripe rust and application of the TaGAT2-1B gene, and the nucleotide sequence of the TaGAT2-1B gene is shown as SEQ ID NO.3. It is found that the TaGAT2-1B gene and the encoding protein thereof can effectively improve the wheat stripe rust resistance, the TaGAT2-1B gene is a new stripe rust resistance gene in the wheat adult-plant stage, infection of various stripe rust strains can be effectively resisted, the field phenotype of the TaGAT2-1B gene shows the characteristic of slow rust, and the TaGAT2-1B gene and the encoding protein thereof have good application prospects. The discovery of the gene provides a new genetic resource for the research of the wheat stripe rust resistance mechanism and the wheat stripe rust resistance breeding.

The invention belongs to the technical field of genetic engineering, and relates to application of dihydroxy acid dehydratase AcDHAD in improvement of disease resistance of kiwi fruits. The invention discloses a dihydroxy acid dehydratase AcDHAD gene and a dihydroxy acid dehydratase AcDHAD protein coded by the dihydroxy acid dehydratase AcDHAD gene through excavation and functional verification, and confirms the broad-spectrum disease-resistant potential of the dihydroxy acid dehydratase AcDHAD in disease-resistant breeding of kiwi fruits. According to the invention, more gene resources are provided for molecular breeding of disease-resistant varieties of kiwi fruits, so that more opinions and ideas are provided for disease-resistant breeding of kiwi fruits and development of molecular markers.

The invention relates to the technical field of biology, in particular to a primer combination for identifying Leyping spotted pigs and an identification method of the primer combination, six specific SNP molecular markers (SNP1-SNP6) are obtained through whole genome screening, six pairs of specific PCR primers are designed and developed on the basis of the sites, the nucleotide sequences of the primers are shown as SEQ ID NO.1-12, and the primer combination is used for identifying the Leyping spotted pigs. And when the genotypes of the to-be-detected sample at the SNP1-SNP6 sites are CC, TT, TT, AA, CC and GG respectively, the to-be-detected sample can be confirmed as a purebred individual of the Licping spotted pig. Compared with a traditional phenotype identification method, the molecular marker detection system established by the invention has the advantages of high detection efficiency, good accuracy and flux. The technical scheme provided by the invention provides important technical support for genetic resource conservation, variety purity identification and genetic improvement of the Leyping spotted pigs, and has remarkable application value in the field of livestock breeding.

The invention discloses application of a watermelon WRKY transcription factor ClWRKY60 in drought stress resistance, and belongs to the technical field of gene engineering. The invention discloses an application of a watermelon WRKY transcription factor ClWRKY60 in drought stress resistance. The nucleotide sequence of the watermelon WRKY transcription factor ClWRKY60 is as shown in SEQ ID NO. 1. The WRKY transcription factor ClWRKY60 is a positive regulation factor of watermelon responding to drought stress, the gene can provide gene resources for creation of drought-resistant new germplasm and breeding of special high-resistance new varieties, and meanwhile, the gene has very important scientific and production application significance for promoting resistance breeding, improving economic benefits of watermelons and the like.

The invention relates to the technical field of biological genetic engineering, and discloses application of cytoplasm 5 '-nucleotidase in prevention and treatment of wheat scab. The invention finds that after the TaNT5C2 gene is over-expressed in wheat, the infection degree of the wheat scab can be reduced, and the wheat scab resistance can be obviously improved. The invention provides a gene resource and a technical thought for gibberellic disease-resistant breeding improvement of wheat.

The invention discloses application of excellent natural variation of a GmPOD123 gene in drought resistance regulation and control of a soybean germination period, and relates to the technical field of biology. According to the application disclosed by the invention, the GmPOD123 gene is subjected to germination period drought resistance phenotype correlation analysis in 400 parts of soybean germplasm resource populations, so that the GmPOD123 gene is excavated and verified to have excellent natural variation which is remarkably related to the soybean germination period drought resistance. A further research result shows that a transgenic line of the over-expression GmPOD123HapG (HapG, Chr17: 14532734) gene can promote soybean germination under drought stress, and it is proved that the gene can improve the drought resistance of the soybean in the germination period. Therefore, the peroxidase gene GmPOD123 disclosed by the invention not only enriches candidate gene resources for improving the drought resistance of the soybean in the germination period, but also provides a candidate molecular marker for cultivating new germplasm of the drought-resistant soybean.

The invention belongs to the technical field of biological gene engineering, and particularly relates to application of CpJAZ13 and a recombinant overexpression vector thereof in improving drought stress resistance of summer squashes, and the nucleotide sequence of the CpJAZ13 is shown as SEQ ID NO.1. According to the invention, overexpression of CpJAZ13 is realized in zucchini through a ZYMV virus mediated overexpression system for the first time, and positive influence of CpJAZ13 on drought resistance of zucchini is confirmed. The expression of the CpJAZ13 is up-regulated after drought stress treatment. The drought resistance of the yeast is improved by overexpressing the CpJAZ13; instantaneous overexpression of the CpJAZ13 increases the oxidation resistance of the summer squash, the ROS accumulation amount is reduced, and the antioxidant enzyme activity is enhanced, so that the resistance of the summer squash to drought stress is increased. Compared with a tedious period of traditional cross breeding, the breeding time can be shortened to several months. According to the invention, a new genetic resource is provided for the improvement of zucchini varieties, and the research and development of new varieties of high-quality and drought-resistant zucchini can be promoted, so that the agricultural production and market requirements are met.

The present disclosure provides novel methods for en bloc transformation or genetic modification of a population of embryonic explants derived from different genetic resources or having different genotypes, which may include one or more steps of explant preparation, explant rehydration, inoculation and co-cultivation with Rhizobiales bacteria or particle bombardment, germination induction, expansion germination induction, and / or regeneration or development of a genetically modified plant or plant part. The methods provided herein may include transforming at least one plant cell of the embryonic explant with a heterologous polynucleotide. The methods provided herein also include methods for regenerating or growing a plurality of genetically modified plants or plant parts from a population of transformed or edited plant cells or explants, and comparing, selecting, or screening for one or more genotypes having improved phenotypes or other culture characteristics.

The invention discloses a marine buffalo whole genome SNP (Single Nucleotide Polymorphism) molecular marker combination and application. The SNP molecular marker combination is shown in a table 1 and is composed of 47943 sequencing source SNP sites with high genetic information content, 868 character related sites and 76 defect related sites, and the total number of the sites is 48887. The marine buffalo whole genome SNP molecular marker combination can be used for designing marine buffalo whole genome chips and protecting and breeding marine buffalo genetic resources, targeted gene detection work can be achieved for marine buffalo populations, various requirements in genetic resource protection and breeding are met, the molecular breeding efficiency is effectively improved, and the breeding cost is reduced. The method is of great significance to protection, evaluation and utilization of genetic resources of the marine buffalo.

The invention provides application of a poplar PtoHsfB1 gene in plant growth, and belongs to the technical field of gene engineering. The function of promoting plant growth of the populus tomentosa gene PtoHsfB1 is found for the first time. Experimental results show that the expression quantity of the PtoHsfB1 gene in the transgenic populus tomentosa is greatly improved compared with that of non-transgenic populus tomentosa, the height, dry weight and fresh weight of a transgenic plant are higher than those of a non-transgenic plant, ABA synthesis and signal transduction can be reduced through overexpression of the gene PtoHsfB1, growth and development of the populus tomentosa are promoted, and the yield of the populus tomentosa is increased. Therefore, a new genetic resource is provided for improvement and molecular breeding of the poplar and other woody plants.