337 results about "Genetic resources" patented technology

The invention belongs to the field of molecular biological identification, and particularly relates to a specific SNP (Single Nucleotide Polymorphism) site combination for identifying a Wuzhishan pig variety and application. The specific SNP site combination for identifying the Wuzhishan pig variety comprises 77 SNP sites, and the physical positions of the SNP sites are determined by sequence comparison based on a pig reference genome Sscrofa11.1. The specific SNP site combination is screened based on a method of combining whole genome association analysis with selection signal analysis so as to ensure the accuracy of site selection. The selected specific SNP site combination can rapidly realize accurate identification of the Wuzhishan pig variety on the gene level, and has significant application value in the aspects of genetic resource accurate protection and variety utilization of Hainan Wuzhishan pigs.

The invention discloses a gene PtoMYB20 for regulating and controlling salt tolerance and drought resistance of poplars. A nucleotide sequence of the gene comprises a sequence as shown in SEQ ID NO. 1. The gene interference fragment is introduced into a plant to obtain a silent transgenic plant, under the conditions of salt stress, drought stress and salt-drought composite stress, the H2O2 and MDA contents of the silent transgenic plant are lower than those of a wild plant, and the SOD and POD activities of the silent transgenic plant are higher than those of the wild plant, so that the damage degree of the plant is effectively reduced; the method is of great significance in improving salt tolerance and drought resistance of the poplar and relieving damage of adversity stress to forestry yield. The invention also discloses a breeding method of the transgenic poplar with strong salt tolerance and drought resistance, provides a new poplar germplasm which not only improves the stress resistance of the poplar but also does not influence the wood yield, and lays a theoretical and gene resource foundation for the stress-resistant molecular breeding of the poplar.

The present invention discloses a gene, GmCDK8, that negatively regulates soybean resistance to Phytophthora spp., and its applications. The disclosed GmCDK8 knockout soybean plants exhibit enhanced resistance to the fungus. Specifically, the results of the soybean cultivar "Williams 82," which lacks GmCDK8, under Phytophthora stress, including root lesion length, leaf lesion area, Phytophthora accumulation, and infected necrotic area on leaves, indicate that knocking out GmCDK8 significantly improves plant resistance to Phytophthora spp. Genetic engineering of this gene for plant resistance to Phytophthora root rot not only provides an important genetic resource for plant molecular breeding but also offers an effective means for achieving high plant yields.

The invention belongs to the field of plant genetic engineering, and discloses application of a PtNF-YB9 gene of trifoliate orange in drought-resistant genetic improvement of plants. The PtNF-YB9 gene is a transcription factor gene which is separated and cloned from Poncirus trifoliata, and the sequence of the PtNF-YB9 gene is as shown in SEQ ID NO. 1. The gene or the homologous gene thereof is knocked out or inhibited in a plant, the drought resistance of the plant is remarkably reduced, and the drought resistance of the plant is remarkably enhanced by overexpressing the gene or the homologous gene thereof. The development and utilization of the genetic resource are beneficial to reducing the agricultural production cost and realizing the green and environment-friendly agricultural goal.

The invention discloses application of an OsCPN10a gene in regulation and control of vigor, grain shape and drought tolerance of rice seeds, and belongs to the technical field of rice gene engineering. The invention particularly relates to the research of the OsCPN10a gene in the aspects of regulating and controlling the germination rate, the germination potential, the germination index, the grain length, the grain width, the thousand grain weight, the drought tolerance of the rice seeds and the like. Experimental results show that the grain shape of the OsCPN10a knockout mutant seed is obviously lengthened and widened, and the grain shape of the OsCPN10a overexpression seed is opposite; the yield of the Ocpn10a mutant is higher than that of a TNG67 plant; overexpression of the OsCPN10a has the potential of improving the drought resistance of the rice. The invention reveals that OsCPN10a affects genetic mechanisms such as rice seed vigor, grain length, grain width and storability, provides important gene resources and theoretical basis for genetic improvement of rice, and is beneficial to cultivation of a new rice variety with better grain shape and higher yield.

The invention belongs to the technical field of agricultural biology, and particularly relates to a transcription factor StBSB with a regulation effect on steroid glycoalkaloid accumulation of potatoes and application of the transcription factor StBSB, the transcription factor StBSB is encoded by a potato gene Soltu.DM.05G001620, and the nucleotide sequence of the potato gene Soltu.DM.05G001620 is shown as SEQ ID NO.1. The invention further discloses a preparation method of the transcription factor StBSB. According to the application, a new insight is provided for understanding a transcriptional regulation mechanism of SGAs in solanaceae plants, and a key theoretical basis and genetic resources are provided for directionally improving the content of anti-nutritional compounds through genetic means.

The invention relates to a wheat stem rot regulatory gene Tatrx-m, and an encoding protein, a recombinant vector and an application of the wheat stem rot regulatory gene Tatrx-m. The genomic sequence of the gene is SEQ ID NO.1, the CDS sequence of the gene is SEQ ID NO.2, and thioredoxin shown as SEQ ID NO.3 is encoded. The invention further provides a specific primer pair used for gene identification, a silent vector (Tatrx-m-VIGS) and an overexpression vector (LGY-OE3-Tatrx-m) are constructed, and the function of the primer pair is verified through a virus-induced gene silencing (VIGS) technology and agrobacterium-mediated transformation of wheat. Experiments show that silence of Tatrx-m results in significant reduction of wheat stem rot resistance, and overexpression of the gene can improve resistance. Based on hypha quantification, DAB staining, H2O2 content and cell death analysis, Tatrx-m is revealed to enhance wheat resistance by regulating active oxygen removal and cell wall strengthening pathways. The gene TaTrx-m has the positive regulation effect in wheat stem rot for the first time, can be applied to disease-resistant molecular marker development, gene editing and transgenic breeding, provides core genetic resources for disease-resistant variety breeding, and has theoretical and application values.

The invention belongs to the technical field of plant molecular biology, and discloses an application of a soybean GmSW10 gene in reducing soybean plant height, grain weight and grease content and / or increasing protein content. The CDS sequence of the soybean GmSW10 gene is as shown in SEQ ID NO: 1. Knockout of the soybean GmSW10 gene can directly influence the plant height, the grain weight, the oil content and the protein content of soybean seeds, after the soybean GmSW10 gene is knocked out, the weight of soybean seeds and hundred grains is remarkably reduced, the plant height is reduced, the protein content is increased, and the oil content is reduced, which indicates that the GmSW10 gene is a key gene for regulating and controlling the plant height, the grain weight, the oil content and the protein content of the soybean, and the GmSW10 gene can be used for preparing the soybean GmSW10 gene for regulating and controlling the plant height, the grain weight, the oil content and the protein content of the soybean. And a new genetic resource is provided for genetic improvement of soybeans.

The invention provides a local livestock and poultry variety identification method based on an SNP marker, and relates to the field of variety identification, and the method comprises the following steps: S1, genotype data acquisition, S2, genotype data quality control, S3, variety identification standard library establishment, and S4, bloodline content evaluation. According to the method, on the basis of a standardized molecular identification analysis process of genomic SNP marker bloodline content evaluation, genotype information of a core SNP site is analyzed, so that whole analysis process processing from original genotyping data to variety identity judgment is realized; the method can provide standardized, convenient and efficient molecular detection technical support for protection and utilization of genetic resources of local livestock and poultry in China, germplasm innovation and industrial development.

The invention relates to the technical field of biology, in particular to a primer combination for identifying Leyping spotted pigs and an identification method of the primer combination, six specific SNP molecular markers (SNP1-SNP6) are obtained through whole genome screening, six pairs of specific PCR primers are designed and developed on the basis of the sites, the nucleotide sequences of the primers are shown as SEQ ID NO.1-12, and the primer combination is used for identifying the Leyping spotted pigs. And when the genotypes of the to-be-detected sample at the SNP1-SNP6 sites are CC, TT, TT, AA, CC and GG respectively, the to-be-detected sample can be confirmed as a purebred individual of the Licping spotted pig. Compared with a traditional phenotype identification method, the molecular marker detection system established by the invention has the advantages of high detection efficiency, good accuracy and flux. The technical scheme provided by the invention provides important technical support for genetic resource conservation, variety purity identification and genetic improvement of the Leyping spotted pigs, and has remarkable application value in the field of livestock breeding.

The invention discloses application of a watermelon WRKY transcription factor ClWRKY60 in drought stress resistance, and belongs to the technical field of gene engineering. The invention discloses an application of a watermelon WRKY transcription factor ClWRKY60 in drought stress resistance. The nucleotide sequence of the watermelon WRKY transcription factor ClWRKY60 is as shown in SEQ ID NO. 1. The WRKY transcription factor ClWRKY60 is a positive regulation factor of watermelon responding to drought stress, the gene can provide gene resources for creation of drought-resistant new germplasm and breeding of special high-resistance new varieties, and meanwhile, the gene has very important scientific and production application significance for promoting resistance breeding, improving economic benefits of watermelons and the like.

The invention discloses a method for separating and transplanting spermatogonial stem cells of Chinese sturgeons, and belongs to the technical field of separation of germline stem cells of fishes. The separation method comprises the following steps: washing Chinese sturgeon testis tissues with an L-15 culture medium containing penicillin / streptomycin double antibodies, and carrying out physical shearing, digestive enzyme enzymolysis and screen filtration to obtain a Chinese sturgeon testis cell suspension; performing percoll density gradient centrifugation to obtain high-purity spermatogonial stem cells of the Chinese sturgeons; and dyeing, washing, resuspending and carrying out micro-injection transplantation to enter the gonad primordium reproductive ridge part of the juvenile acceptor fish. According to the physical characteristics of the spermatogonial stem cells of the Chinese sturgeons, the spermatogonial stem cells with high purity are obtained by adopting percoll density gradient centrifugation, and the purpose of abdomen borrowing reproduction is achieved by adopting a microinjection mode, so that the preservation of germ cells of endangered fishes and the reconstruction of artificial populations are realized; and a new technical approach is provided for protecting genetic resources of the Chinese sturgeons and breaking through artificial propagation bottlenecks.

The invention provides application of a transcription factor LcNF-YB3 of lycoris chinensis in regulating and controlling the flowering time of plants, and belongs to the technical field of molecular breeding. The invention provides a lycoris chinensis transcription factor LcNF-YB3. The amino acid sequence of the lycoris chinensis transcription factor LcNF-YB3 is as shown in SEQ ID NO: 1. The Lycoris chinensis transcription factor LcNF-YB3 delays plant flowering through forward regulation, the flowering time of an arabidopsis thaliana plant for heterologous expression of LcNF-YB3 is delayed, rosette leaves are increased, and the flowering time of the plant Lycoris chinensis is obviously advanced by silencing the LcNF-YB3 gene in Lycoris chinensis. The Lycoris chinensis transcription factor LcNF-YB3 can effectively regulate and control the flowering time, solves the problems of asynchronous flowering phases and the like in breeding, and provides a new genetic resource for the improvement of Lycoris and other bulb flower varieties.

The invention discloses a tomato Solyc08g008190 gene and a tomato Solyc08g008200 gene and application of the tomato Solyc08g008190 gene and the tomato Solyc08g008200 gene. A CDS (Coding Sequence) sequence of the Solyc08g008190 gene is shown as SEQ ID NO. 1, and a CDS sequence of the Solyc08g008200 gene is shown as SEQ ID NO. 2. A tomato transporter mutant library is constructed through a CRISPR (clustered regularly interspaced short palindromic repeats) technology, and a mutant 0990-32 which simultaneously covers expression of two homologous genes of an NHE family is screened. Based on observation of the phenotype of the mutant, the inventor preliminarily determines that the two genes are related to tomato fruit quality and fruit volume increase. Compared with a wild type, the tomato fruit of the mutant has the advantages that the brix content is increased, and the fruit becomes larger. It is shown that the Solyc08g008190 gene and the Solyc08g008200 gene play an important role in regulating and controlling the growth, development and quality of the tomato fruits. The invention lays a good gene genetic resource foundation for the cultivation of new varieties of high-quality tomatoes.

The invention discloses a CAPS molecular marker of a GhFRO7 gene of upland cotton and application of the CAPS molecular marker in early blossoming character identification and breeding, and belongs to the field of molecular biology. Whole genome association analysis is carried out based on a high-density SNP marker, an SNP site which is located on an upland cotton GhFRO7 gene and is remarkably related to the flowering time character is identified and obtained, and then a CAPS molecular marker with the sequence shown as SEQ ID NO.34 and a primer pair with the sequence shown as SEQ ID NO.3-4 are developed. The CAPS molecular marker and the primer pair thereof provided by the invention can be applied to identification of early flowering upland cotton varieties and molecular marker-assisted selective breeding, and can effectively save the detection cost, shorten the period and improve the detection efficiency. The invention provides a new method for genetic improvement of cotton prematurity, and provides important genetic resources for developing cotton varieties adapting to specific climate conditions.

The invention belongs to the technical field of agricultural biology, and particularly discloses a molecular marker site related to gray mold resistance of Chinese roses and application of the molecular marker site. Specifically, the invention provides application of an SNP site in Chinese rose gray mold resistance molecular marker assisted breeding. The SNP loci are located at the 74bp site and the 108bp site from the 5'end of a sequence shown as SEQ ID NO.1, and when basic groups of the two loci present GT, the germplasm is judged as the germplasm with high gray mold resistance. Through analysis of a plurality of germplasm resources, it is found that the haplotype is GT, the germplasm has a smaller scab area and shows higher gray mold resistance, and the germplasm with high gray mold resistance is obtained. The method is mainly derived from genetic resources of Asian wild type and ancient Chinese varieties, and by means of the technical scheme, the breeding process can be accelerated, and Chinese rose germplasm with disease resistance can be rapidly obtained.

The invention discloses an application of a Solyc02g060560 gene in regulation and control of sugar content and size of tomato fruits, wherein a CDS (Coding Sequence) sequence of the Solyc02g060560 gene is as shown in SEQ ID NO. 1. A tomato transcription factor mutant library is constructed through a CRISPR (clustered regularly interspaced short palindromic repeats) technology, and a mutant TOM6-126 with unknown functions at present in a C2H2 family is screened. Based on observation of the phenotype of the mutant, the inventor determines that the gene is related to the sugar content and the fruit size of tomato fruits. Compared with a wild type, the soluble solid content (brix) of tomato fruits of the mutant is increased, and the fruits become larger. It is indicated that the Solyc02g060560 gene plays an important role in negative regulation of tomato fruit growth and development and sugar content. According to the invention, a good gene genetic resource foundation is laid for the cultivation of a new variety of tomatoes with high sugar content.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a wheat gene to regulation and control of plant drought tolerance. The wheat gene provided by the invention is a TaANK-TPR44 gene, and the nucleotide sequence of the TaANK-TPR44 gene is as shown in SEQ ID NO. 1. In order to develop the specific functional characteristics of ankyrin genes from different plant sources, the TaANK-TPR44 gene is over-expressed and transformed into arabidopsis thaliana for functional verification, and the result shows that the drought tolerance of the arabidopsis thaliana with the over-expressed TaANK-TPR44 gene is remarkably improved compared with that of wild arabidopsis thaliana, and the drought tolerance of the arabidopsis thaliana with the over-expressed TaANK-TPR44 gene is remarkably improved compared with that of wild arabidopsis thaliana. Therefore, the TaANK-TPR44 gene plays a positive regulation role in the wheat drought tolerance process, a theoretical basis and gene resources are provided for wheat molecular breeding, and the TaANK-TPR44 gene has very important significance for obtaining drought-tolerant high-yield strains and improving the wheat drought tolerance in the future.

The invention belongs to the technical field of biology, and discloses a cattle SNP chip and application thereof, the detection object of the liquid phase chip comprises 7,829 SNP sites located on a common cattle reference genome AR-UCD 1.2, and the position information of the SNP sites is shown in the specification table 3. The liquid chip provided by the invention highlights the specificity of the local cattle variety in the Tibet autonomous region, and by analyzing the local cattle population structure, sites capable of effectively distinguishing the population are screened, so that the problems of strong universality and poor specificity of a commercial chip are solved. The liquid-phase chip can also realize flexible selection and upgrading of sites, and breaks through the limitation that a solid-phase chip cannot flexibly supplement or update the sites. The whole method can be stably operated on the premise of limited sample size and complex population structure, and is suitable for genetic resource protection and application scenarios of rare varieties. The method can also be expanded into a standardized typing tool, and is suitable for various applications such as local cattle germplasm registration, variety identification, region identification and the like.

The invention relates to the field of genetic engineering and molecular biology, in particular to application of an SlGT2 gene in regulation and control of plant dwarfing. The nucleotide sequence of the SlGT2 gene is a nucleotide sequence as shown in SEQ ID NO: 1. The expression level of the SlGT2 gene is regulated and controlled through an SlGT2 overexpression plant so as to research the regulation and control mechanism of the SlGT2 gene on the tomato plant height. Results show that stem cells of the SlGT2 overexpressed tomato plant become smaller, the stem node spacing is shortened, and the plant height is reduced. Gene resources are provided for cultivating new tomato dwarfing varieties, potential application value is achieved, and meanwhile a theoretical basis is laid for researching a tomato dwarfing molecular mechanism.

The invention discloses application of a CG4 gene in regulating and controlling cadmium transport of plants at high temperature, and belongs to the technical field of gene engineering. The nucleotide sequence of the CG4 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the protein coded by the CG4 gene is as shown in SEQ ID NO: 2. The invention provides a novel related gene for regulating and controlling the cadmium transport of the rice at the high temperature, a good research basis is provided for the cadmium transport research of the rice at the high temperature, and a novel genetic resource is also provided for cultivating a low-cadmium variety of the rice.

The invention relates to a method for extracting genome DNA of plants rich in secondary metabolites and a buffer solution, and belongs to the technical field of molecular biology and botany. The method solves the technical problems of low DNA extraction efficiency, poor purity, easy degradation and the like when a traditional DNA extraction method is used for treating plant tissues rich in secondary metabolites such as alkaloid, polyphenol and the like. Comprising the following steps: adding a complexing agent such as polyethylene glycol or polyvinylpyrrolidone when grinding plant tissues in a liquid nitrogen environment; splitting by using a cell wall splitting buffer solution containing dithiothreitol and a nonionic surfactant; carrying out DNA release and extraction at 60-70 DEG C by adopting a CTAB (Cetyltrimethyl Ammonium Bromide) extraction buffer solution containing polyethylene glycol and papain; and then purifying and precipitating to obtain high-purity genome DNA (Deoxyribose Nucleic Acid). The method can effectively remove alkaloid, protein and other impurities, significantly improves the DNA yield and purity, and is suitable for genome sequencing, genetic resource protection, medicinal plant molecular identification and the like of plants with high secondary metabolites such as Stephania kwangsiensis and the like.

A use of a ZmCCD8 gene in maize breeding, including overexpressing the ZmCCD8 gene in maize plants through breeding to increase iron content in maize leaves and / or kernels. The ZmCCD8 gene has a nucleotide sequence as shown in SEQ ID NO: 1. The use of the ZmCCD8 gene in maize breeding can provide important genetic resources and technical support for improving crop quality and enhancing stress resistance, having important application prospects.

The present invention belongs to the field of plant genetic engineering technology, and in particular to a rice cytokinin response regulator gene OsRR24 And application, the rice cytokinin response regulatory factor gene OsRR24 It encodes a protein containing a DNA binding domain and a conserved aspartate-containing signal receiving domain, and is a response regulator downstream of the rice CK signaling pathway. OsRR24 During the functional process in rice, it was transferred into Nipponbare and Bph6 In the NIL, overexpressing plants showed significantly enhanced resistance to brown planthoppers, while RNAi-inhibited plants showed significantly reduced resistance to brown planthoppers. The gene of this invention provides a good theoretical basis for studying how cytokinins participate in rice insect resistance through response regulatory factors. It has reference significance for studying gene molecular functions and also provides genetic resources for the design and breeding of insect-resistant rice varieties.

The invention belongs to the field of biotechnology and livestock breeding, and relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to sheep meat quality traits, a primer group, a kit and application. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO.4, and Ggt exists at the 46th bp position of the sequence as shown in SEQ ID NO.4; a is a mutation site. By rapidly and accurately detecting the SNP marker related to the meat quality traits of the'Auodurou Lake 'sheep genetic resource population, the'Auodurou Lake' sheep variety dominant genetic resource population can be rapidly established, so that the marker-assisted selection breeding process of the sheep meat quality traits is accelerated.

The invention relates to the technical field of plant genetic engineering, in particular to a ZmbHLH166 gene related to drought resistance in the flowering stage of corn, an encoding protein and application of the ZmbHLH166 gene. The key effect of the ZmbHLH166 gene in the drought resistance of the corn in the flowering period is identified for the first time on the basis of whole genome association analysis (GWAS) and transgenic function verification, and through experiments, through genetic transformation and continuous selfing screening of a Ubi1: ZmbHLH166 overexpression vector, the obtained T2-generation corn strain of the overexpression ZmbHLH166 gene has the advantages that compared with a wild type corn strain, the yield is increased, and the yield is increased. The ZmbHLH166 gene has a shorter pollen scattering-spinning interval, which indicates that under drought stress, the overexpression of the ZmbHLH166 gene can effectively promote the synchronous development of male and female flowers of corn, thereby enhancing the drought resistance of crops. Therefore, the application of the ZmbHLH166 gene and the protein coded by the ZmbHLH166 gene in regulating and controlling the drought resistance of the corn in the flowering period is feasible, and a new and reliable gene resource and an improvement direction are provided for drought-resistant breeding of the corn.

The application discloses a transcription factor for regulating protein content of rice grains GPC6 and application, the amino acid sequence of the transcription factor is shown as SEQ ID NO. 1, the transcription factor comprises a genomic region and a promoter, the nucleotide sequence of the genomic region is shown as SEQ ID NO. 2, the nucleotide sequence of the promoter is shown as SEQ ID NO. 3, the coding sequence (CDS) is shown as SEQ ID NO. 4, and all alleles thereof are contained. The application identifies a repeatedly detectable locus on chromosome 6 by performing whole genome association analysis on gluten content and total protein content GPC6 . Gene cloning is carried out on the locus, so that the genetic resources for improving rice quality are enriched, and help is provided for improving rice quality, in particular, protein content.

The invention relates to the technical field of Bian chicken source lactobacillus sharianus, in particular to Bian chicken source lactobacillus sharianus YYBJ-07-01 as well as a preparation method and application of the Bian chicken source lactobacillus sharianus YYBJ-07-01. The Bian chicken source lactobacillus shariensis YYBJ-07-01 is preserved in the China Center for Type Culture Collection on September 23, 2025, the classification name of the Bian chicken source lactobacillus shariensis YYBJ-07-01 is that the Bian chicken source lactobacillus shariensis YYBJ-07-01 is classified and named, and the preservation number of the Bian chicken source lactobacillus shariensis YYBJ-07-01 is CCTCC (China Center for Type Culture Collection) No.M20252093. As a national protection variety, the strong environmental adaptability of Bian chickens is associated with a unique intestinal microbiome. According to the invention, not only is the precious genetic resource of Bian chicken deeply excavated and scientifically utilized, but also a solid material foundation is laid for developing a Bian chicken source lactobacillus sharianus YYBJ-07-01 product, and the application value of a local characteristic variety is improved.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a chrysanthemum indicum CzNAC1 gene and application thereof, and the nucleotide sequence of the gene is as shown in SEQ ID NO.1. According to the invention, transcriptome data is screened and cloned to the drought-responsive CzNAC1 gene, a carrier is constructed, and a transgenic plant is cultivated, so that the cultivation of a specific variety of transgenic chrysanthemum indicum, drought resistance conditions, economic and social benefits increase, stress-resistant genes are explored, gene resource mining and cross-species application are further promoted, and the application prospect is wide. And a good basis is provided for subsequent gene technology research.

This invention belongs to the field of genetic engineering, specifically involving LbMYB88 Gene or LbMYB88 Application of gene overexpression vectors in improving plant resistance to drought stress. This invention utilizes a newly created... LbMYB88 Overexpression of the gene in wolfberry plants was observed after drought treatment. LbMYB88 The overexpression of the gene in wolfberry plants showed significant resistance compared to the wild type, providing genetic resources for breeding stress-resistant wolfberry varieties and possessing good potential application value. It also lays a theoretical foundation for studying the molecular mechanism of drought resistance in wolfberry.