186 results about "Genetic resources" patented technology

The invention belongs to the field of molecular biological identification, and particularly relates to a specific SNP (Single Nucleotide Polymorphism) site combination for identifying a Wuzhishan pig variety and application. The specific SNP site combination for identifying the Wuzhishan pig variety comprises 77 SNP sites, and the physical positions of the SNP sites are determined by sequence comparison based on a pig reference genome Sscrofa11.1. The specific SNP site combination is screened based on a method of combining whole genome association analysis with selection signal analysis so as to ensure the accuracy of site selection. The selected specific SNP site combination can rapidly realize accurate identification of the Wuzhishan pig variety on the gene level, and has significant application value in the aspects of genetic resource accurate protection and variety utilization of Hainan Wuzhishan pigs.

The invention belongs to the field of plant genetic engineering, and discloses application of a PtNF-YB9 gene of trifoliate orange in drought-resistant genetic improvement of plants. The PtNF-YB9 gene is a transcription factor gene which is separated and cloned from Poncirus trifoliata, and the sequence of the PtNF-YB9 gene is as shown in SEQ ID NO. 1. The gene or the homologous gene thereof is knocked out or inhibited in a plant, the drought resistance of the plant is remarkably reduced, and the drought resistance of the plant is remarkably enhanced by overexpressing the gene or the homologous gene thereof. The development and utilization of the genetic resource are beneficial to reducing the agricultural production cost and realizing the green and environment-friendly agricultural goal.

The invention belongs to the technical field of agricultural biology, and particularly relates to a transcription factor StBSB with a regulation effect on steroid glycoalkaloid accumulation of potatoes and application of the transcription factor StBSB, the transcription factor StBSB is encoded by a potato gene Soltu.DM.05G001620, and the nucleotide sequence of the potato gene Soltu.DM.05G001620 is shown as SEQ ID NO.1. The invention further discloses a preparation method of the transcription factor StBSB. According to the application, a new insight is provided for understanding a transcriptional regulation mechanism of SGAs in solanaceae plants, and a key theoretical basis and genetic resources are provided for directionally improving the content of anti-nutritional compounds through genetic means.

The invention belongs to the technical field of plant molecular biology, and discloses an application of a soybean GmSW10 gene in reducing soybean plant height, grain weight and grease content and / or increasing protein content. The CDS sequence of the soybean GmSW10 gene is as shown in SEQ ID NO: 1. Knockout of the soybean GmSW10 gene can directly influence the plant height, the grain weight, the oil content and the protein content of soybean seeds, after the soybean GmSW10 gene is knocked out, the weight of soybean seeds and hundred grains is remarkably reduced, the plant height is reduced, the protein content is increased, and the oil content is reduced, which indicates that the GmSW10 gene is a key gene for regulating and controlling the plant height, the grain weight, the oil content and the protein content of the soybean, and the GmSW10 gene can be used for preparing the soybean GmSW10 gene for regulating and controlling the plant height, the grain weight, the oil content and the protein content of the soybean. And a new genetic resource is provided for genetic improvement of soybeans.

The invention provides application of a transcription factor LcNF-YB3 of lycoris chinensis in regulating and controlling the flowering time of plants, and belongs to the technical field of molecular breeding. The invention provides a lycoris chinensis transcription factor LcNF-YB3. The amino acid sequence of the lycoris chinensis transcription factor LcNF-YB3 is as shown in SEQ ID NO: 1. The Lycoris chinensis transcription factor LcNF-YB3 delays plant flowering through forward regulation, the flowering time of an arabidopsis thaliana plant for heterologous expression of LcNF-YB3 is delayed, rosette leaves are increased, and the flowering time of the plant Lycoris chinensis is obviously advanced by silencing the LcNF-YB3 gene in Lycoris chinensis. The Lycoris chinensis transcription factor LcNF-YB3 can effectively regulate and control the flowering time, solves the problems of asynchronous flowering phases and the like in breeding, and provides a new genetic resource for the improvement of Lycoris and other bulb flower varieties.

The invention belongs to the technical field of agricultural biology, and particularly discloses a molecular marker site related to gray mold resistance of Chinese roses and application of the molecular marker site. Specifically, the invention provides application of an SNP site in Chinese rose gray mold resistance molecular marker assisted breeding. The SNP loci are located at the 74bp site and the 108bp site from the 5'end of a sequence shown as SEQ ID NO.1, and when basic groups of the two loci present GT, the germplasm is judged as the germplasm with high gray mold resistance. Through analysis of a plurality of germplasm resources, it is found that the haplotype is GT, the germplasm has a smaller scab area and shows higher gray mold resistance, and the germplasm with high gray mold resistance is obtained. The method is mainly derived from genetic resources of Asian wild type and ancient Chinese varieties, and by means of the technical scheme, the breeding process can be accelerated, and Chinese rose germplasm with disease resistance can be rapidly obtained.

The invention discloses application of a CG4 gene in regulating and controlling cadmium transport of plants at high temperature, and belongs to the technical field of gene engineering. The nucleotide sequence of the CG4 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the protein coded by the CG4 gene is as shown in SEQ ID NO: 2. The invention provides a novel related gene for regulating and controlling the cadmium transport of the rice at the high temperature, a good research basis is provided for the cadmium transport research of the rice at the high temperature, and a novel genetic resource is also provided for cultivating a low-cadmium variety of the rice.

The invention relates to a method for extracting genome DNA of plants rich in secondary metabolites and a buffer solution, and belongs to the technical field of molecular biology and botany. The method solves the technical problems of low DNA extraction efficiency, poor purity, easy degradation and the like when a traditional DNA extraction method is used for treating plant tissues rich in secondary metabolites such as alkaloid, polyphenol and the like. Comprising the following steps: adding a complexing agent such as polyethylene glycol or polyvinylpyrrolidone when grinding plant tissues in a liquid nitrogen environment; splitting by using a cell wall splitting buffer solution containing dithiothreitol and a nonionic surfactant; carrying out DNA release and extraction at 60-70 DEG C by adopting a CTAB (Cetyltrimethyl Ammonium Bromide) extraction buffer solution containing polyethylene glycol and papain; and then purifying and precipitating to obtain high-purity genome DNA (Deoxyribose Nucleic Acid). The method can effectively remove alkaloid, protein and other impurities, significantly improves the DNA yield and purity, and is suitable for genome sequencing, genetic resource protection, medicinal plant molecular identification and the like of plants with high secondary metabolites such as Stephania kwangsiensis and the like.

The invention relates to the technical field of plant genetic engineering, in particular to a ZmbHLH166 gene related to drought resistance in the flowering stage of corn, an encoding protein and application of the ZmbHLH166 gene. The key effect of the ZmbHLH166 gene in the drought resistance of the corn in the flowering period is identified for the first time on the basis of whole genome association analysis (GWAS) and transgenic function verification, and through experiments, through genetic transformation and continuous selfing screening of a Ubi1: ZmbHLH166 overexpression vector, the obtained T2-generation corn strain of the overexpression ZmbHLH166 gene has the advantages that compared with a wild type corn strain, the yield is increased, and the yield is increased. The ZmbHLH166 gene has a shorter pollen scattering-spinning interval, which indicates that under drought stress, the overexpression of the ZmbHLH166 gene can effectively promote the synchronous development of male and female flowers of corn, thereby enhancing the drought resistance of crops. Therefore, the application of the ZmbHLH166 gene and the protein coded by the ZmbHLH166 gene in regulating and controlling the drought resistance of the corn in the flowering period is feasible, and a new and reliable gene resource and an improvement direction are provided for drought-resistant breeding of the corn.

The invention relates to the technical field of Bian chicken source lactobacillus sharianus, in particular to Bian chicken source lactobacillus sharianus YYBJ-07-01 as well as a preparation method and application of the Bian chicken source lactobacillus sharianus YYBJ-07-01. The Bian chicken source lactobacillus shariensis YYBJ-07-01 is preserved in the China Center for Type Culture Collection on September 23, 2025, the classification name of the Bian chicken source lactobacillus shariensis YYBJ-07-01 is that the Bian chicken source lactobacillus shariensis YYBJ-07-01 is classified and named, and the preservation number of the Bian chicken source lactobacillus shariensis YYBJ-07-01 is CCTCC (China Center for Type Culture Collection) No.M20252093. As a national protection variety, the strong environmental adaptability of Bian chickens is associated with a unique intestinal microbiome. According to the invention, not only is the precious genetic resource of Bian chicken deeply excavated and scientifically utilized, but also a solid material foundation is laid for developing a Bian chicken source lactobacillus sharianus YYBJ-07-01 product, and the application value of a local characteristic variety is improved.

The present application belongs to the field of plant genetic engineering and biotechnology, and particularly relates to a method for creating a dwarf material of Brassica napus by using CRISPR / Cas9 gene editing technology and application. The method edits the Brassica napus BnaA06G0083400WE gene by using CRISPR / Cas9 gene editing technology to obtain a dwarf material of Brassica napus; the nucleotide sequence of the coding gene mutant of the Brassica napus dwarf material is SEQ ID NO. 1. The present application edits the Brassica napus BnaA06G0083400WE gene by using CRISPR / Cas9 gene editing technology to obtain a dwarf Brassica napus, which provides valuable genetic resources and germplasm resources for Brassica napus breeding; the method has strong characteristics and is easy to obtain, is an effective way to realize the improvement of target traits and cultivate new materials, and can be applied to Camellia sinensis breeding.

The invention provides an oocyte in-vitro maturation culture solution additive and application thereof. The additive is tert-butylhydroquinone. By adding 100 [mu] g / mL of TBHQ into a basic culture solution, the first polar body discharge rate of oocytes is remarkably increased to 77.29%, the ROS level is reduced, and the blastocyst rate of in-vitro fertilized embryos is increased. The technology is suitable for breed conservation of livestock genetic resources such as sheep and can replace live livestock transportation to reduce the cost.

The invention discloses an application of a TaERF9 gene in cooperation with histone modification to participate in nitrogen-mediated root development. The homologous genes of three subgenomes of the TaERF9 gene respectively correspond to the GenBank numbers of CM022223.1, CM022224.1 and CM022225.1 in NCBI (National Center of Biotechnology Information). According to the invention, wheat KN199 is directionally edited by using a CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-associated protein 9) technology, so that a mutant plant with TaERF9 function deletion is obtained. Protein interaction experiments prove that the TaERF9 can interact with the subunit SWN of the PRC2. The results of high-nitrogen and low-nitrogen water culture experiments show that TaERF9 gene mutation significantly promotes root development under a high-nitrogen condition; under the low-nitrogen condition, the root development of the mutant is kept similar to that of a wild type, the ability of balancing the root development under different nitrogen supply conditions is shown, and the effect of the TaERF9 gene in nitrogen response mediated root development regulation is revealed. That is to say, the TaERF9 gene can promote root development after mutation, so that a gene resource is provided for nitrogen-efficient breeding of crops.

The invention belongs to the technical field of gene engineering, and particularly relates to a larch drought response gene LgLEA10, a protein and application thereof. The nucleotide sequence of the larch drought response gene LgLEA10 provided by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of the protein expressed by the larch drought response gene LgLEA10 is as shown in SEQ ID NO.2. It is proved for the first time that the LgLEA10 gene of dahurian larch is a drought response regulation gene of dahurian larch. Tests prove that overexpression of the LgLEA10 gene can improve the drought resistance of larch by remarkably improving the activity of antioxidant enzyme and osmotic adjusting substances. The discovery provides important gene resources and theoretical basis for cultivating a new variety of larch with strong drought resistance.

The invention belongs to the technical field of biology, and relates to application of a ZmCASPL11 gene in improving heat resistance of plants. The ZmCASPL11 gene encodes a protein of a membrane protein (CASPL) family related to a Kjeldahl band structure, the gene expression level of the ZmCASPL11 gene under high-temperature stress is remarkably increased, and a mutant zmcaspl11 of the gene shows a high-temperature sensitive phenotype. The ZmCASPL11 gene disclosed by the invention is transformed into arabidopsis thaliana and is subjected to functional verification, and compared with wild type arabidopsis thaliana, the tolerance of an arabidopsis thaliana plant over-expressed with the ZmCASPL11 gene to heat stress is remarkably enhanced. According to the invention, more theoretical bases are provided for corn heat resistance research, and gene resources and biotechnological means are provided for subsequent cultivation and germplasm resource innovation of new heat-resistant corn strains.

This invention discloses a SlZHD11 The application of genes in regulating crop yield and flavor quality falls under the field of genetic engineering technology. This invention utilizes CRISPR / Cas9 gene editing technology to insert endogenous genes from tomatoes... SlZHD11 Knockout in tomatoes significantly increases fruit size, yield, and sugar content. Without affecting the normal growth of the tomato plant, it improves both fruit yield and quality, providing genetic resources and strategies for tomato germplasm innovation and better meeting the needs of industry and consumers.

The invention discloses a probe for detecting a penaeus japonicus SNP molecular marker combination, a penaeus japonicus 31K liquid phase breeding chip and application, and belongs to the technical field of molecular marker breeding. The penaeus japonicus SNP molecular marker combination is composed of 31097 SNP molecular markers. The 31097 SNP molecular markers are as shown in the specification table 1. The invention also provides application of the penaeus japonicus liquid phase breeding chip in penaeus japonicus breeding, sample detection, genetic resource genetic diversity analysis, genetic and evolutionary analysis, genetic relationship identification, whole genome association analysis and penaeus japonicus genome selection. The penaeus japonicus 31K liquid phase breeding chip provided by the invention has the characteristics of good site representativeness, strong specificity, high polymorphism and the like, is widely applicable to detection of different varieties of penaeus japonicus materials, can establish a modern breeding system, and promotes the development of the penaeus japonicus molecular breeding industry.

The invention relates to the technical field of sugarcane cross breeding, and particularly discloses a sugarcane cross combination selection method. The sugarcane hybrid combination matching method comprises the following steps: S1, carrying out parent multi-dimensional character evaluation: respectively carrying out genotype data on candidate sugarcane male parents and candidate sugarcane female parents according to agronomic characters, quality characters and stress resistance characters of the candidate sugarcane male parents and candidate sugarcane female parents; s2, intelligent selective mating of the hybridized combination; s3, evaluating and determining a combined value; s4, parent treatment and flowering phase regulation before hybridization; s5, artificial cross pollination; and S6, screening filial generations. The sugarcane hybrid combination matching method can be used for directional breeding of new sugarcane varieties, genetic resource optimal configuration and rapid screening of high-yield and high-sugar hybrid combinations, and has the advantages of high prediction precision, short breeding period and high resource utilization efficiency by combining multi-dimensional character evaluation with an intelligent prediction model.

This invention belongs to the field of agricultural biotechnology, specifically relating to a type of sisal. DREB Genes and their application in improving plant cold resistance. This invention provides sisal. DREB The gene expression level increased significantly after low-temperature stress, indicating a close relationship between its expression and the response of sisal to low-temperature stress. The study found that transgenic methods can significantly improve the expression level of sisal. DREB Genes were overexpressed, along with sisal DREB Increased gene expression levels led to improved cold resistance in transgenic Arabidopsis thaliana. Overexpression of sisal in plants also improved cold resistance. DREB The discovery that genes can enhance plant cold resistance can be used to breed new varieties or seedlings of cold-resistant plants, providing a theoretical basis and genetic resources for genetic breeding, which is of great significance to agricultural production.

The invention discloses separation and application of a vibrio parahaemolyticus lytic bacteriophage vBVpaSR27Z (Vibrio parahaemolyticus lytic bacteriophage). The preservation number of the bacteriophage vBVpaSR27Z is GDMCC No: 67438-B1 (GDMCC No: 1). The bacteriophage is separated from an environmental sample by adopting a double-layer plate method, the head of the bacteriophage is in a regular icosahedron shape (84 nm * 71 nm), the tail length of the bacteriophage is about 141 nm, the incubation period of the bacteriophage is only 20 min, the lysis speed is high, and the activity of the bacteriophage is stable within the pH range of 5-10 and the temperature range of 4-37 DEG C; the genome of the vibrio bacteriophage is double-stranded DNA without known drug resistance and toxicity genes, phylogenetic analysis shows that the vibrio bacteriophage has low homology with known vibrio bacteriophages, and the vibrio bacteriophage belongs to a novel genetic resource. The vBVpaSR27Z can rapidly inhibit the growth of strains such as vibrio parahaemolyticus, is suitable for green prevention and control of diseases such as vibrio parahaemolyticus in aquaculture, and can be used as an active component of a biological antibacterial preparation.

The application discloses a Cmfsg gene mutant of melon and a creation method and application thereof, and belongs to the technical field of plant genetic engineering. The mutant is obtained by CRISPR / Cas9 gene editing technology to direct mutation of the coding region or the promoter region of the Cmfsg gene of melon, the target point is recognized by a specific sgRNA primer, and is screened by specific primer PCR amplification and sequencing verification. The creation method comprises the following steps: constructing an editing vector of a target gene, introducing the wild-type queen melon by means of an agrobacterium-mediated genetic transformation technology, screening, regeneration and molecular identification to obtain the mutant. The Cmfsg gene has a regulation function on the fruit fur character and the fruit shape index of melon, can realize the direct and rapid creation of the appearance character of melon, overcomes the defects of long breeding period and low efficiency in traditional breeding, and the obtained mutant has stable genetic traits, and can be used as a parent or genetic resource for high-quality breeding of melon.

The invention provides an OsWRKY19 gene and an application of the OsWRKY19 gene in resistance to cochliobolus miyabeanus of rice. A CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 (CRISPR associated protein 9) technology is adopted to obtain an OsWRKY19 knockout mutant (Oswrky19) and an overexpression strain of And after the cochliobolus miyabeanus pathogenic bacteria are inoculated, compared with a wild type, the knockout mutant is more susceptible to diseases, and the over-expression strain resistance is obviously enhanced. The OsWRKY19 overexpression obviously improves the callose deposition level after rice plants are induced by chitin, and further enhances the basic defensive reaction of rice. RT-qPCR discovers that the expression of the defensive reaction related gene OsMBL1 in a knockout mutant is reduced and is increased in an overexpression plant, which indicates that the OsWRKY19 can promote the expression of the OsMBL1, thereby enhancing the resistance of rice. In conclusion, the OsWRKY19 is a gene for positively regulating and controlling the resistance of the cochliobolus miyabeanus of the rice, and can promote the basic defense response of the rice and the expression of a disease-resistant related gene, so that the resistance of the rice to the cochliobolus miyabeanus is improved. The content of the invention provides important genetic resources and theoretical guidance for the breeding of the cochliobolus miyabeanus resistant variety of rice.

The invention belongs to the technical field of gene engineering, and particularly relates to application of an expression promoter of a PubHLH47 gene to improvement of salt stress resistance of populus japonica, and the nucleotide sequence of the PubHLH47 gene is as shown in SEQ ID NO.1. The PubHLH47 gene provided by the invention is utilized to construct a plant expression vector, so that the PubHLH47 gene is over-expressed in the populus japonica, and the salt stress resistance of the populus japonica is improved. A PubHLH47 gene overexpressed plant has the characteristic of effectively improving the tolerance of the plant under the stress of high salt. The PubHLH47 gene capable of improving the salt stress resistance is found in forest for the first time, an excellent gene resource is provided for promoting forest genetic engineering breeding, and a scientific basis is provided for later improvement of populus nigra.

The application belongs to the technical field of molecular biology, and particularly relates to a macadamia SNP molecular marker combination and application thereof. The SNP molecular marker combination comprises 284 SNP sites, the 284 SNP sites can uniformly cover a macadamia genome, have high polymorphism information content, and can be used for constructing a macadamia DNA fingerprint map, and on the basis of the DNA fingerprint map, the SNP molecular marker combination can be further used for identifying macadamia germplasm resources, and can solve problems such as mixed germplasm in resources, repeated preservation and management, and intellectual property right disputes. Meanwhile, the 284 SNP sites are used for screening macadamia core germplasm, so that the most abundant genetic diversity of a species can be maximally represented by the least number of germplasm and the lowest genetic redundancy, the management and utilization efficiency of the germplasm resources is improved, the genetic resources are effectively protected and utilized, and useful genetic resources are selected for a macadamia breeding plan.

The application provides a cold-resistant gene TrWRKY10a540, an expression vector, application and breeding method thereof, and belongs to the field of plant molecular genetics and genetic improvement. Specifically disclosed is a cold-resistant gene TrWRKY10a540 and an expression vector derived from Trifolium repens L. and application thereof in improving the cold resistance of plants, and the problem of insufficient research on low-temperature stress related genes of Trifolium repens in the prior art is solved. The application further discloses a breeding method for regulating the cold stress resistance of plants by the cold-resistant gene TrWRKY10a540, and research results show that the gene can significantly improve the cold resistance and antioxidant level of plants under low-temperature stress conditions, and has good functional effects. The application can be widely applied to plant molecular breeding, is particularly suitable for improving the low-temperature resistance of economic crops such as forage grasses, provides a new genetic resource for the breeding of cold-resistant plants, and has important significance for revealing the regulation mechanism of the cold resistance of plants.

The invention belongs to the field of plant genes, and relates to application and a method of a GmTOC1a gene or protein in regulation and control of soybean flowering. The application of the soybean biological clock gene GmTOC1a in flowering control is found, two homologous copies GmTOC1a1 (Glyma. 05G025000) and GmTOC1a2 (Glyma. 17G102200) of the GmTOC1a gene are directionally knocked out through a CRISPR / Cas9 gene editing technology, a soybean mutant with the GmTOC1a gene mutated is obtained, research finds that the mutant shortens the growth period of soybeans and advances the flowering time, and it is indicated that the GmTOC1a inhibits soybean flowering. Therefore, the GmTOC1a gene can be applied to change or expand the cultivation regionalism of soybeans, so that the soybeans can be sown at different latitudes, the planting region of the soybeans is expanded, and a theoretical basis and genetic resources are provided for breeding good varieties of the soybeans.

This invention belongs to the field of biotechnology and relates to a... ZmCASPL11 The application of genes in improving plant heat tolerance. ZmCASPL11 The gene encodes a protein belonging to the Casparian band structure family of membrane proteins (CASPL), whose expression level is significantly upregulated under high temperature stress. Mutants of this gene... ZmCASPL11 It exhibits a high-temperature sensitive phenotype. The present invention describes... ZmCASPL11 Genes were transformed into Arabidopsis thaliana and their function was validated. Compared with wild-type Arabidopsis thaliana, overexpression of [the gene] showed [significant improvement]. ZmCASPL11 The gene significantly enhances the tolerance of Arabidopsis thaliana plants to heat stress. This invention provides more theoretical basis for research on heat resistance in maize, and offers genetic resources and biotechnological means for the subsequent breeding of new heat-resistant maize lines and the innovation of germplasm resources.

The invention discloses a method for improving wheat scab resistance by mutating a heavy metal binding protein TaHMAP. According to the invention, a homozygous three-mutant strain KO-1-3 of the TaHMAP gene is created, and the gibberellic disease resistance of a TaHMAP gene function deletion mutant is found to be improved. The study on the lignin synthesis pathway gene in the mutant finds that the expression of the lignin regulation gene is improved after the TaHMAP gene mutation. Lignin content determination shows that the lignin content of the homozygous three mutants of the TaHMAP gene is obviously higher than that of a wild plant. Therefore, the TaHMAP mutant strain has potential value in the aspects of analyzing a wheat gibberellic disease resistant signal channel and improving the resistance of wheat to gibberellic disease, and can be used as a genetic resource for wheat gibberellic disease resistant breeding.

This invention belongs to the field of genetic engineering technology, specifically relating to improving the drought resistance of larch trees. LgERF4 Genes and their applications. (The following is a separate section...) LgERF4 The nucleotide sequence of the gene is shown in SEQ ID NO.1. This invention is the first to experimentally demonstrate... LgERF4 Gene overexpression can enhance the drought resistance of larch by significantly increasing the activity of antioxidant enzymes and the content of osmotic regulators. This discovery provides important genetic resources and theoretical basis for breeding new larch varieties with strong drought resistance.

The invention relates to the technical field of plant genetic engineering and molecular breeding, in particular to application of CmBt and CmBr genes in regulation and control of bitter taste of muskmelon fruits. Physical interaction exists between muskmelon fruit bitter taste regulation factors CmBt and CmBr proteins, and expression of a bitter taste synthesis key gene CmBi is synergistically activated; the CmbtCmbr double mutants are constructed through a gene editing technology, it is proved that double-gene knockout has a synergistic effect, and the content of cucurbitacine B in all tissue of muskmelon can be more remarkably reduced; field trials show that under induction of forchlorfenuron, bitter taste of fruits of the CmbtCmbr double mutant completely disappears, the effect of the CmbtCmbr double mutant is remarkably superior to that of a single-gene mutant, and quality characters such as fruit weight and sugar degree are not negatively influenced; effective gene resources and germplasm materials are provided for solving the industrial problem that fruits become bitter due to the fact that plant growth regulators are used in muskmelon production.