The invention belongs to the technical field of plant tissue culture and particularly relates to a factory seedling cultivating method of actinidia arguta. The method is mainly technically characterized by comprising the five steps of: preparing culture media, selecting and disinfecting explants, inoculating, subculturing and transplanting in a sand bed. The factory seedling cultivating method of actinidia arguta is mainly provided on account of the conditions that actinidia arguta is in a wild state in all northeast areas, and the traditional cultivating modes which comprise sowing propagation, cuttage propagation and grafting propagation cause the problems that the variation of offspring characters is serious, male plants are difficult to separate from female plants, resources are seriously damaged, the seedling cultivating speed is low, the characters of the female plants are difficult to keep, the plants are easily infected with bacteria, the survival rate of the seedlings is low, and the like. Induced buds are subcultured and then directly transplanted in the sand bed, the rooting culture process is omitted, the seedling cultivating period is shortened by 50-60 days, the seedling cultivating cost is greatly lowered, the survival rate of the seedlings is obviously increased, the plant infection rate is reduced, excellent female plant characters are kept, and the variety degrading problem is solved.