99 results about "Coprecipitation" patented technology

The invention discloses a magnetic biochar coupled dynamic membrane synergistic pollution reduction and flora regulation method, which comprises the following steps: adding magnetic biochar into an anaerobic dynamic membrane bioreactor for anaerobic digestion reaction, and adding 1.2-1.5 g of magnetic biochar into each liter of sludge mixed liquid in the anaerobic dynamic membrane bioreactor. The magnetic biochar is composed of a honeycomb-shaped carbon material obtained by pyrolyzing carbon through sodium citrate and Fe3O4 loaded on the surface of the honeycomb-shaped carbon material through a chemical coprecipitation method. According to the method, functional microorganisms such as methane filamentous bacteria and methane rod bacteria can be selectively enriched, the methane yield is increased by 30%-35%, membrane pollution is effectively relieved by improving the sludge floc structure, the service life of the membrane is prolonged, and technical support is provided for low-cost and low-energy-consumption operation of the anaerobic membrane bioreactor.

The invention provides a precursor containing primary particles distributed in the radial direction and a preparation method and application thereof.The preparation method comprises the steps that a mixed metal source solution, a complexing agent solution and a precipitator solution are subjected to a first coprecipitation reaction, and a core is obtained; in oxygen-free protective gas, increasing the flow of the mixed metal source solution, the complexing agent solution and the precipitator solution, and carrying out second coprecipitation reaction to obtain a core coated with a compact layer; in an oxygen-containing atmosphere, introducing the mixed metal source solution, the complexing agent solution, the precipitator solution and the doped metal source solution, and carrying out a third co-precipitation reaction to obtain a precursor; the pH and complexing agent concentration of the third co-precipitation reaction are smaller than those of the second co-precipitation reaction and are smaller than those of the first co-precipitation reaction. According to the preparation method, the precursor comprises the primary particles distributed along the radial direction by regulating and controlling the conditions of the coprecipitation reaction at each stage, so that the performances such as circulation and rate of the positive electrode material are improved.

The application belongs to the technical field of gas sensing material preparation, and discloses an Ag / AgCl modified Co3O4 nano-composite material, a preparation method and application, and comprises the following steps: dissolving inorganic compounds of Ag elements and inorganic compounds of Co elements in deionized water, adding a proper amount of NaCl solution first, then adding N2H4.H2O solution, and obtaining Ag / AgCl loaded alpha-Co(OH)2 nanosheet material by using coprecipitation and in-situ reduction method; and drying and sintering the Ag / AgCl loaded alpha-Co(OH)2 nanosheet material to obtain the Ag / AgCl modified Co3O4 nano-composite material. The application provides a preparation method of a low-temperature high-response gas sensing material, the prepared Ag / AgCl loaded Co3O4 material has higher ethanol gas response performance, can significantly reduce the optimal working temperature of the sensing material, has potential application prospect, and can be used for monitoring alcohol pollutant gases in atmospheric environment, chemical plant environment, home environment and the like.

The invention belongs to the technical field of drug carriers, and particularly relates to a magnetic nano-drug carrier which is prepared by the following steps: selecting traditional Chinese medicine raw materials and auxiliary raw materials, and selecting corresponding equipment for treating the raw materials; traditional Chinese medicine raw materials are pretreated, effective components are extracted through ultrasonic and reflux combination, and finally an extracting solution is purified and concentrated; preparing through a coprecipitation method; the flexible traditional Chinese medicine dressing is prepared by taking anti-inflammatory, analgesic, blood-activating and blood-stasis-removing active ingredients of 19 traditional Chinese medicines as cores and combining with the local magnetic response enrichment characteristic of the magnetic nano-carrier, the flexible traditional Chinese medicine dressing is attached to a pressurized silica gel pad of a PCI postoperative wrist strap for use, local directional enrichment of the active ingredients of the traditional Chinese medicines in a wound is promoted through the magnetic carrier, and the wound healing effect is improved. The pressurizing silica gel pad has the synergistic effects of resisting inflammation, easing pain, improving microcirculation and dredging collaterals and restoring the pulse, solves clinical pain points such as inflammation edema, obvious pain and unsmooth local blood supply of a wrist puncture wound after the PCI operation, and meets the requirements of flexibility, ventilation and fitting of the pressurizing silica gel pad at the same time.

The invention provides a core-shell structure composite flame-retardant synergist, a preparation method and application thereof, an intumescent fireproof coating, and preparation and application thereof, and belongs to the technical field of fire-fighting materials. Through a coprecipitation method, ammonium polyphosphate APP and melamine MEL which exist independently traditionally are compounded and assembled at a molecular level on the surface of a zinc borate particle serving as an anchor point to form a core-shell integrated functional particle. At the high temperature of a fire, due to close contact and extremely short reaction path of APP and MEL of the shell layer, violent dehydration, gasification and cross-linking reaction can be instantly generated, strong expansion driving force with more controllable directionality is generated, the expansion efficiency is greatly improved, and the coating fire resistance, carbon layer quality and durability of the fireproof coating can be remarkably improved.

The present invention belongs to the technical field of sensorineural hearing. Provided is the use of SPTBN1 as a target in the treatment of GJB2-related sensorineural hearing loss. The use comprises: the construction of a stably transfected cell line, wherein: HEK293T cells are cultured using a DMEM culture medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin in a humidified incubator at 37ºC with 95% air and 5% CO2, and when the cells reach 80%-90% confluence, cell passage is performed; IP-MS analysis, wherein: protein complexes are purified using Protein A / G immunoprecipitation magnetic beads, a portion of the extracted proteins is used as input, and then 2 μg of anti-Cx26 antibody is added to the remaining protein extract, same are gently pipetted and mixed, and incubated on a rotating shaker at 4℃ overnight; immunofluorescence observation of the co-localization of Cx26 and SPTBN1, wherein: a stably transfected cell line expressing WT-Cx26 and Mut-Cx26 is constructed; and co-immunoprecipitation (Co-IP) validation, wherein: a stably transfected cell line expressing WT-Cx26 is constructed. The present invention overcomes the limitations of therapeutic methods, such as the relatively low targeting specificity and short therapeutic time windows associated with full-length protein supplementation via gene therapy, thereby providing new insight into GJB2-related hearing loss, and a new target and a new treatment for same.

In order to solve the technical problems of difficult dewatering of fluorine-containing sludge and difficult recycling of ordinary chemical filter aids, the application relates to a reusable filter aid for filtering and dewatering fluorine-containing sludge and a preparation method thereof. The main component of the filter aid is alkyl carboxylic acid modified magnetic ferroferric oxide nanoparticles. The preparation method comprises the following three steps: (1) preparing magnetic ferroferric oxide nanoparticles by using a coprecipitation method, (2) amino modification of the surface of the magnetic ferroferric oxide nanoparticles, and (3) alkyl carboxylic acid modification of the magnetic ferroferric oxide nanoparticles. Compared with the prior art, the application has the advantages of simple preparation method, excellent filter aid effect (significantly shortened filtering time and significantly reduced water content of sludge filter cake), stable reuse performance and the like, and has a good application prospect.

The invention discloses superfine high-dispersion type nano TiO2 as well as a preparation method and application thereof, and the preparation method of the superfine high-dispersion type nano TiO2 comprises the following steps: adding a TiCl4 solution into a base solution containing ammonium polycarboxylate, polyether modified siloxane and ammonia water, and carrying out coprecipitation reaction to prepare the nano TiO2. According to the preparation method, through the synergistic effect among ammonium polycarboxylate, polyether modified siloxane and ammonia water in the base solution, nano TiO2 which is good in dispersity, uniform in particle size, spherical in microstructure, 5-10 nm in particle size and has a mixed phase of a rutile phase and an anatase phase is prepared, the preparation process is mild in reaction condition, simple in equipment requirement, short in process route and suitable for industrial production, and the preparation method is suitable for industrial production. The industrial production is easy, and the application field of the superfine high-dispersion type nano TiO2 is greatly widened.

The application discloses a preparation method and application of a rubidium and cesium adsorbent, and relates to the mixing of a certain amount of a complexing agent, a salt solution A and a salt solution B, the regulation of the coprecipitation reaction process of the salt solution A and the salt solution B by the complexing agent, and the preparation of the rubidium and cesium adsorbent. The rubidium and cesium adsorbent prepared by the method has high adsorption capacity, high adsorption selectivity and high stability, and can realize the effective separation of rubidium and cesium from brine.

A preparation method of a medium-entropy perovskite oxygen carrier comprising: 1) preparing an aqueous solution from metallic nitrate serving as a raw material, performing a coprecipitation reaction w

A single crystal NCMA quaternary precursor and a preparation method thereof.A single crystal NCMA quaternary precursor has a chemical formula of Ni a Al b Zr c (OH)2·Ni d Co e Mn f (OH)2.The preparation method comprises the following steps: one, preparing a first metal liquid of Ni, Zr and a complexing agent A, preparing a second metal liquid of Ni, Co, Mn and H2O2, preparing a precipitant, preparing a complexing agent B, and preparing a mixed solution; two, simultaneously adding the first metal liquid, the precipitant and the mixed solution into a kettle for co-precipitation, the volume of the first metal liquid added per hour is 6-9% of the effective volume of the kettle, and the volume of the mixed solution added per hour is 1.5-2.2%; the solid content is controlled to be 1.2-1.3 g / mL, the pH value is kept at 11.8-12.2, and the concentration of the complexing agent A is 0.1-0.2 mol / L; a core layer Ni a Al b Zr c (OH)2 is obtained after T1 hours; the adding of the first metal liquid and the mixed solution is stopped, the second metal liquid and the complexing agent B are changed, reaction is carried out for T2 hours, the D50 of the slurry reaches 3.1-3.9 um, and the reaction is stopped; three, the co-precipitation product is subjected to pressure filtration, washing and drying to obtain the single crystal NCMA quaternary precursor.The Al in the single crystal NCMA positive electrode material prepared by the method is uniformly distributed, and the electrical performance is good.

The invention provides a photolysis group-containing antibody, a method and a kit for co-immunoprecipitation sequencing. The photolysis group-containing antibody comprises a first antibody and a linker reagent label, and the first antibody is subjected to photolysis-bifunctional labeling through a linker reagent; the linker reagent comprises three active groups R1, R2 and R3; wherein R1 is used for covalent linkage with a first antibody; r2 is a photolysis group and fractures under illumination of a preset wavelength, so that connection between R1 and R3 is broken; r3 is used for carrying out binding reaction with a solid-phase substrate; r1, R2 and R3 are connected through a length-adjustable flexible chain, and the length-adjustable flexible chain is of a polyethylene glycol or carbon chain structure; according to the method, interference of complex backgrounds in cell samples can be reduced, the sample input quantity is reduced, and low-abundance epigenetic modification is realized.

The invention discloses a rare earth oxygen storage material preparation device and method based on a coprecipitation method, and relates to the technical field of material preparation.The rare earth oxygen storage material preparation device comprises a precipitation tank and a base fixedly connected to the lower surface of the precipitation tank and used for keeping the device stable, and a drain valve penetrates through the bottom end of the outer surface of the precipitation tank. The precipitation tank is used as a main aging and standing precipitation space for accommodating a rare earth salt solution and a precipitation product, and is provided with a drain valve for discharging the precipitation product generated by standing of the precipitation tank; the mixing mechanism is used for mixing and stirring a solution, the mixing box is arranged on the outer surface of the mixing mechanism, meanwhile, a pH detector is arranged in an inner cavity of the mixing box, the pH value of the internal solution can be monitored in real time, and the mixing mechanism is arranged to serve as a core stirring assembly to be used for fully mixing and stirring the solution; the effect of slowly and uniformly dripping the precipitant into the rare earth salt solution is achieved.

The application discloses an LDH-based vaccine adjuvant and a preparation method and application thereof, and belongs to the technical field of aluminum adjuvant preparation of vaccines, and the preparation method comprises the following steps: mixing at least one of soluble magnesium salt, soluble zinc salt, soluble manganese salt and soluble calcium salt with soluble aluminum salt, and then performing a co-precipitation reaction with a sodium hydroxide aqueous solution in a colloid mill device, and performing crystallization to obtain LDH nanosheets; dispersing the LDH nanosheets in a PBS solution, adding a hepatitis B surface antigen molecule drug, uniformly mixing, and then performing centrifugation to obtain the LDH-based vaccine adjuvant. The LDH-based vaccine adjuvant can significantly promote the maturation of DC cells and the activation of T cells, and the total IgG level reflects the overall humoral immune response intensity of the body to vaccines or pathogens; the LDH-based vaccine adjuvant forms a "storage library" at an injection site to store antigens and slowly release the antigens, and the purpose of long-time continuous stimulation of the immune system in the body is achieved.

The application discloses a preparation method of hollow double-shell ZnSnO3 / SnO2 heterojunction sensor material and application of the material in selective detection of triethylamine, and belongs to the technical field of gas sensors. The preparation method of the hollow double-shell ZnSnO3 / SnO2 heterojunction sensor material comprises the following steps: ZnSn(OH)6 / SnO2.nH2O microsphere precursors are prepared by using a coprecipitation method; the ZnSn(OH)6 / SnO2.nH2O microsphere precursors are added into an organic solvent to perform a solvothermal reaction, and then are calcined to obtain the hollow double-shell ZnSnO3 / SnO2 heterojunction sensor material. The hollow double-shell ZnSnO3 / SnO2 heterojunction sensor material can be used for high-response detection of triethylamine at a low working temperature, has excellent selectivity and stability, and can be used for actual monitoring application of food freshness.

The present invention discloses a synthesis method for a layered dimetal-based nano lanthanum material capable of synchronously locking phosphorus, removing algae, and reducing turbidity, and the use thereof, and belongs to the field of environmental functional materials. In the present invention, a synthesis method includes: synthesizing LDHs by means of a single-drop coprecipitation method; filtering out an obtained product, washing the product with distilled water and drying; then adding the product to an alcohol solution of a lanthanum salt at certain concentration, and stirring for reaction; precipitating lanthanum ions in situ by adjusting a pH value of the solution; filtering out the reaction precipitate, washing the precipitate with an alcohol, and drying the precipitate to obtain an LDH-based nano lanthanum material.

The application discloses a CTAB modified magnetic waste fungus chaff biochar adsorbent and a preparation method and application thereof in pyrethroid detection. 3+ , Fe 2+ and the fungus chaff biochar, adding an alkaline reagent to carry out a coprecipitation reaction to obtain a magnetic biochar precursor; S3, pyrolyzing the magnetic biochar precursor to obtain a magnetic biochar; S4, adding the magnetic biochar into a cetyltrimethylammonium bromide solution to carry out ultrasonic treatment, and carrying out magnetic separation to obtain an adsorbent MBC-CTAB. The application establishes a pyrethroid analysis method in water samples by taking the MBC-CTAB as the adsorbent, is favorable for utilization of the waste fungus matrix, and adopts a magnet to separate the sample solution and the MBC-CTAB, replaces complicated separation equipment, and realizes convenient and rapid separation.

This invention belongs to the field of environmental monitoring and forensic science testing, specifically relating to a magnetic walnut shell biochar material, its preparation method, and its application. The preparation method involves first pulverizing the walnut shell biochar, modifying it with phosphoric acid, and then loading it with Fe3O4 via a co-precipitation method to obtain the magnetic material. Using this as a magnetic solid-phase extraction adsorbent, combined with gas chromatography-mass spectrometry (GC-MS), it can achieve efficient enrichment and accurate detection of 11 cathinone-type new psychoactive substances in environmental water and urine. The detection process is simple to operate, has a high recovery rate, and strong anti-interference ability.

The invention discloses a method for purifying a tin-containing high-concentration iron-aluminum solution. The method comprises the following steps: step 1, preparing the tin-containing high-concentration iron-aluminum solution; 2, iron and aluminum solution pretreatment; step 3, preparing a precipitant; step 4, removing tin; 5, collecting and treating tin-containing precipitates; step 6, carrying out post-treatment on the solution; in the second step, an iron plate, an aluminum plate or a tin plate is directly added into the iron-aluminum solution in the first step, stirring is conducted under the normal temperature condition, and the plate is taken out after pretreatment is completed; the precipitant in the step 3 comprises a liquid precipitant and a solid precipitant; according to the method, a coordination binuclear / multinuclear hydrolysis effect is generated, Sn coprecipitation removal is promoted, and iron and aluminum cannot be greatly lost; the preferable organic compounding agent can promote the generation of metal-Orgn < + >, enhance the electric neutralization effect of the ligand and improve the hydrolysis coprecipitation effect; and the use of the organic ingredient helps tin precipitate in the solution to generate loose precipitate, and is easy to filter.

The application relates to the technical field of permanent ferrite preparation, in particular to a method for preparing strontium ferrite by a multi-field coupling auxiliary molten salt method. First, a chemical coprecipitation method is improved to obtain a mixture containing an excess precipitant, inorganic salt and strontium ferrite precursor; then the mixture is subjected to heat treatment under multi-field coupling; finally, strontium ferrite powder is obtained after washing and drying. The application simplifies the chemical coprecipitation process, avoids Sr loss and realizes in-situ salt formation; a multi-element molten salt system is constructed during heat treatment, and an alkali-containing molten salt system with activation is proposed; in the molten salt liquid phase environment, multi-physical fields such as magnetic fields and ultrasonic vibration force fields are cooperated to control the orientation and growth of grains, new driving force and fast channels are provided for ion diffusion, and the rapid synthesis of strontium ferrite and the control of microstructure and size are realized in a low temperature and short time.

The invention provides a copper calcium phosphate nano-enzyme fibroin antibacterial dressing as well as a preparation method and application thereof, and belongs to the technical field of material preparation. CCP HNS nano-enzyme with ascorbic acid oxidase is prepared through a coprecipitation method, nano-enzyme particles with controllable morphology, stable structure and excellent catalytic performance are obtained by regulating and controlling the ratio of copper to calcium and reaction conditions, and then the nano-enzyme particles are uniformly loaded on a natural protein material silk fibroin (SF), so that the nano-enzyme is prepared. The calcium copper phosphate nano-enzyme fibroin antibacterial dressing is obtained; the copper calcium phosphate nano-enzyme fibroin antibacterial dressing has an excellent antibacterial effect, is relatively low in cytotoxicity, maintains good cell viability and shows good biocompatibility; the copper calcium phosphate nano-enzyme fibroin antibacterial dressing has obvious advantages in the aspects of infection control, inflammation relief and tissue regeneration, can greatly shorten the wound closing time, shows good healing promoting ability, and has good practicability.

<pre>The present invention relates to an optimized process for manufacturing phosphoric acid by the wet process, aimed at increasing the chemical yield, filterability, density, reducing the sulfate level, and increasing the P₂O₅ titer. In conventional processes, the phosphoric acid obtained has a P₂O₅ concentration of less than 25%, due to the insufficient acid attack of the phosphate and the coprecipitation of the dicalcium phosphate in the gypsum. The innovation lies in the integration of animal bones, selected from bovine, sheep and chicken bones, after pretreatment including calcination, at a rate of 3% by weight of the phosphate pulp, used as a dopant. This incorporation promotes improved crystallization of calcium sulfate, which increases the reaction yield, density, and P₂O₅ titer of the phosphoric acid, while improving filterability and reducing losses. The experimental results show that the use of bone significantly improves the performance of the process.< / pre>

The invention belongs to the technical field of preparation of vaccine immunologic adjuvants, and discloses a surface-modified aluminum hydroxide nanoparticle adjuvant as well as preparation and application thereof. The aluminum hydroxide-based adjuvant is a next-generation aluminum hydroxide-based adjuvant at an optimal level specially optimized for veterinary vaccines. The formula of the adjuvant solves the limitation of the traditional aluminum hydroxide adjuvant, including poor cellular immune induction and variable efficacy in large animals. The adjuvant promotes balanced Th1 / Th2 immune response, reduces local injection site reaction, and improves the shelf life of the vaccine. The preparation method comprises ultrasonic homogenization and co-precipitation under pH control, vaccine compositions containing the adjuvant, and uses of the adjuvant in prevention of infectious diseases in animal husbandry, companion animals and poultry. Experimental data show that in bovine respiratory disease and avian influenza models, compared with traditional adjuvants, the immunogenicity is excellent, the antibody titer is increased by 3 times, and CD8 + T cell response is enhanced.

The application provides a high-efficiency water treatment agent and a preparation method and application thereof, and relates to the technical field of water treatment.The preparation method of the water treatment agent comprises the following steps: (1) mixing nano-Fe3O4, potassium permanganate, manganese salt, potassium persulfate and water, wherein the mass ratio of the potassium permanganate, the potassium persulfate and the nano-Fe3O4 is 150-160:500-700:450-480; (2) performing a catalytic reaction under ultrasonic and ultraviolet conditions and at a temperature of 50-70 DEG C to obtain a composite magnetic Fe3O4-nano-MnO2; (3) separating the solid by using a magnet, washing, drying and obtaining the water treatment agent; and in the step (1), the nano-Fe3O4 is prepared by a coprecipitation method using an iron salt.The water treatment agent has a good treatment effect on high-COD-content wastewater, especially high-salt-and-COD-content wastewater and sewage, and has a high COD removal rate.

The invention discloses a preparation method and application of a low-valence manganese-based anti-ozone aging agent. According to the preparation method, ascorbic acid is used as a main reducing agent and is used for reducing the valence state of Mn; the intercalated manganese-based hydrotalcite is prepared by adopting a coprecipitation method, interlayer guest molecules are complexed with Mn through coordination to further stabilize the reduced Mn, and the average valence state of Mn is as low as 2.4 through the synergistic effect of the interlayer guest molecules and Mn. Experimental results show that the low-valence manganese-based anti-ozone aging agent shows good ozone catalytic decomposition capability, and physical and chemical dual ozone aging resistance can be realized. And the material obtained by compounding the composite material with rubber also shows excellent ozone aging resistance.

A recycling process for Lithium-ion (Li-ion) batteries employs a closely monitored co-precipitation reaction for precipitating cathode active material precursor (pCAM) in a comingled form of metal salts corresponding to the intended chemistry for the new battery. The coprecipitation relies on a controlled pH that assures a particle size, morphology and purity of the resulting precipitated pCAM. A combination of internal reactor pH sensing within the coprecipitation reactor along with external pH sensing to validate the sensed pH ensures a consistent target pH throughout the coprecipitation process, compensating for drift or sensor failure in view of the harsh reactor environment.

The invention relates to the technical field of environmental engineering water treatment, in particular to a method for removing thallium element in water through modified zeolite. The invention aims to solve the technical bottlenecks that when the existing zeolite adsorbent is used for removing thallium, solid-liquid separation is difficult, and the selective adsorption capacity on trace thallium is limited. Therefore, the invention creatively provides a composite treatment method integrating magnetic separation, multistage pore channel adsorption and biological enhancement. The preparation method is characterized in that firstly, a core-shell structure composite material with magnetic Fe3O4 as a core, natural zeolite as a middle layer and an MOF material as a shell layer is prepared through a hydrothermal synthesis and coprecipitation technology, and the material has magnetic response, high specific surface area and high affinity to thallium ions; secondly, performing functional modification on the surface of the composite material, and directionally mounting a specific thallium removal biological membrane to construct an adsorption-biotransformation synergistic system;

A Panax notoginseng polysaccharide iron nanoparticle preparation, its preparation method, and its application are disclosed. This invention can effectively prepare Panax notoginseng polysaccharide iron nanoparticles, solving the application problem in the preparation of drugs for treating iron deficiency anemia, inflammatory bowel disease-related anemia, iron deficiency anemia-related colitis, inflammatory anemia, or inflammatory bowel disease. The specific steps are as follows: The crude Panax notoginseng polysaccharide solution is purified using conventional purification methods to obtain purified Panax notoginseng polysaccharide. This purified polysaccharide is dissolved in ultrapure water, and an iron source is added under inert gas protection. After co-precipitation and chemical reduction reactions, the pH value is adjusted, and iron nanoparticles are loaded onto the Panax notoginseng polysaccharide to obtain Panax notoginseng polysaccharide-coated iron nanoparticles. The preparation method of this invention is simple, uses abundant raw materials, and has low production costs. It represents a major innovation in drugs for treating iron deficiency anemia and inflammatory bowel disease-related iron deficiency anemia, and has practical application value.

The invention relates to the technical field of pharmaceutical preparations, and discloses a doxorubicin and cyclophosphamide liposome compound injection which is prepared from doxorubicin hydrochloride, cyclophosphamide, hydrogenated soybean phosphatidylcholine, cholesterol, polyethylene glycol derivative phospholipid, anhydrous magnesium sulfate, L-arginine and a sucrose octasulfate ammonium solution. Magnesium ions introduced into an inner water phase participate in competitive binding, doxorubicin and sucrose octasulfate are induced to form a loose co-precipitation structure with lattice defects, hydrophilic cyclophosphamide is physically filled and cured by using micro gaps, and efficient encapsulation of non-gradient dependent drugs is realized. Meanwhile, an in-situ pH buffer system is constructed by using L-arginine, protons generated in a drug loading process are neutralized, an internal water phase is maintained in a neutral environment, and acid-catalyzed hydrolysis of cyclophosphamide is effectively inhibited. According to the invention, the double-drug encapsulation efficiency and storage stability of the compound liposome are obviously improved.

The invention discloses a serum purification method based on Protein G affinity layer medium and PEG (Polyethylene Glycol) coprecipitation, which comprises the following steps: putting a Protein G affinity chromatography medium into an empty purification column filled with Binding / Wash Buffer, and naturally settling a filler to the bottom of the column; sequentially adding Binding / Wash Buffer and a serum sample, collecting effluent, repeatedly loading the sample, and collecting the effluent; adding an Elution Buffer for washing, then adding a Binding / Wash Buffer for balancing the filler again, and carrying out regeneration repeatedly; the preparation method comprises the following steps: uniformly mixing with a 20% PEG (Polyethylene Glycol) 8000 solution, incubating at room temperature in a dark place, centrifuging, sucking out supernate, adding the PEG 8000 solution, re-suspending, uniformly mixing, and centrifuging. The method can effectively remove the interference of the pre-stored antibody and improve the detection sensitivity and accuracy, and is simple and convenient to operate, low in cost and wide in applicability.