19 results about "Chitosan microspheres" patented technology

Aiming at the problem that a conventional mask cannot absorb large cells through natural permeation, the invention provides the preparation method of the nano-microsphere cell active factor mask, and the nano-microsphere cell active factor mask is prepared through the processes of preparing an emulsion system, solidifying small liquid drops, alkalizing materials such as polymer collagen / chitosan microspheres, stirring to prepare a nano-microsphere cell active stock solution and the like. According to the prepared polymer collagen / chitosan porous microspheres, cell active factors are diffused into the pore diameters of the microspheres, the permeability of active factor cells is improved through a microsphere carrier, and proliferation and growth of skin epidermal cells are greatly promoted.

The invention belongs to the technical field of food or medicine, and particularly relates to a preparation method of nattokinase sustained-release microspheres, which comprises the following steps: mixing an emulsifier span-80 with liquid paraffin to obtain an oil phase; adding chitosan into an acetic acid solution to prepare a chitosan water solution as a water phase; dropwise adding the water phase into the oil phase to prepare W / O type emulsion, dropwise adding a cross-linking agent A into the emulsion to obtain chitosan microspheres, and taking a chitosan microsphere nattokinase solution and a cross-linking agent B to obtain nattokinase-chitosan core microspheres; mixing the nattokinase-chitosan core microsphere with a cortisol aptamer solution, and incubating to enable the aptamer to be combined on the surface of the nattokinase-chitosan core microsphere; and adding a casein-PEG copolymer solution and a cross-linking agent C into the microspheres to obtain the cortisol response type intelligent switch microspheres. The nattokinase release amount can be dynamically adjusted day and night, the circadian thrombus risk rhythm is matched, the anti-thrombus effect is remarkably improved, side effects are reduced, and the nattokinase can be widely applied to the fields of functional food and drugs.

The invention discloses a neural microelectrode for electrical stimulation combined with drug release and a preparation method thereof.The neural microelectrode comprises a first flexible substrate layer, a drug-loaded slow-release layer, a second flexible substrate layer, a conductive layer and an insulating covering layer, and the drug-loaded slow-release layer contains chitosan microspheres embedded with drugs; the medicament comprises nerve growth factors and / or anti-inflammatory drugs, a plurality of drug release holes are distributed in the insulating covering layer, an electrode contact is arranged at one end of the conducting layer, a lead connector is electrically arranged at the other end of the conducting layer, and openings for exposing the electrode contact and the lead connector are correspondingly formed in the two ends of the insulating covering layer respectively. According to the neural microelectrode provided by the invention, the drug-loaded slow-release layer is integrated in the neural microelectrode, so that a space for storing a drug does not need to be additionally processed on the membrane layer, and the drug does not need to be additionally loaded, so that the production and processing are relatively convenient and rapid; after the neural microelectrode is implanted into a corresponding tissue in a body, the drug-loaded sustained-release layer can be gradually degraded to slowly release a medicament for repairing nervous tissues or avoiding rejection reaction in a long-acting manner.

The application discloses a feed additive for removing microplastics, a preparation method and application. The composition consists of the following components in percentage by weight: modified chitosan microspheres 15-25%, nanoscale yeast cell wall derivatives 10-20%, fermentable dietary fiber complex 15-25%, glutamine 5-10%, complex probiotics and prebiotics 15-25%, and natural plant extract compound antioxidant 5-10%. The additive can be added to the feed at a weight ratio of 0.5-2.0% through a multi-target synergistic mechanism of 'adsorption-promoting excretion-repair-regulation', and can efficiently remove microplastics in aquatic products (the removal rate is up to 65% or more), improve intestinal health, enhance antioxidant function, reduce the residues of heavy metals and other complex pollutants, and significantly improve the edible safety and quality of aquatic products.

The invention discloses a feed additive for removing micro-plastics as well as a preparation method and application of the feed additive. The composition is prepared from the following components in percentage by weight: 15%-25% of modified chitosan microspheres, 10%-20% of a nanoscale yeast cell wall derivative, 15%-25% of a fermentable dietary fiber compound, 5%-10% of glutamine, 15%-25% of compound probiotics and prebiotics and 5%-10% of a natural plant extract compound antioxidant. The additive disclosed by the invention can be added into a feed according to a weight ratio of 0.5-2.0% through a multi-target synergistic mechanism of adsorption, discharge promotion, repair and regulation, so that micro-plastics in cultured aquatic products are efficiently removed (the removal rate is up to 65% or above), meanwhile, the intestinal health is improved, the antioxidant function is enhanced, the residues of composite pollutants such as heavy metals are reduced, and the feed quality is improved. The food safety and the quality of aquatic products are obviously improved.

The application belongs to the technical field of water treatment, and particularly relates to a phosphorus-containing modified magnetic chitosan microsphere, a preparation method thereof and application of the phosphorus-containing modified magnetic chitosan microsphere in treatment of chromium-containing underground water. Compared with the prior art, the phosphorus-containing modified magnetic chitosan microsphere provided by the application takes magnetic nanoparticles as a core, can be quickly separated from a reaction system by relying on external magnetic force of a system, and forms hydrogen bonds through hydroxyl groups of the magnetic nanoparticles and amino groups and hydroxyl groups of a chitosan molecular chain, so that stability and dispersibility of the magnetic nanoparticles are improved. Furthermore, a tetramethylammonium hydroxide solution is used as a crosslinking agent, so that a phosphorus-containing cationic group in the tetramethylammonium hydroxide is grafted on the chitosan molecule, thereby greatly enhancing adsorption performance of the chitosan on anionic heavy metal pollutants such as Cr(VI), and having long-term stability in remediation of Cr(VI)-contaminated underground water.

The invention provides a 3D printing material loaded with a stem cell epimatrix as well as a preparation method and application of the 3D printing material, and belongs to the technical field of biological materials. Comprising the following components in parts by weight: 100 to 150 parts of modified silk fibroin peptide-polylactic resin, 20 to 40 parts of stem cell loaded extracellular matrix nanoparticles, 10 to 20 parts of slow-release chitosan microspheres, 150 to 200 parts of sodium hyaluronate and 50 to 100 parts of gelatin. Compared with a traditional chemically synthesized polymer scaffold, the 3D printing material loaded with the stem cell extracellular matrix prepared by the invention has better biocompatibility, high safety and excellent mechanical properties, promotes adhesion growth of cells, realizes slow and uniform release of drugs, adjusts cartilage growth and ossification by multiple signals, promotes cartilage repair, and has good application prospects. Wide application prospects are realized.

This invention discloses a continuous flow method for preparing chitosan hollow microspheres. The invention utilizes ultrasonic microreactor technology combined with emulsion crosslinking to successfully prepare chitosan hollow microspheres. The hollow chitosan microspheres provided by this invention are suitable for encapsulating various drugs, with a precisely tunable particle size within the range of 200 nm to 1.5 μm. They exhibit excellent biocompatibility and biodegradability, and a high drug loading capacity. The preparation process is a continuous synthesis process, resulting in highly uniform particle size and morphology of the product. The preparation process is safe, efficient, and easily scaled up.

The invention relates to the technical field of medical cosmetology, and particularly discloses biomass microspheres for cosmetic injection and a preparation method of the biomass microspheres. The biomass microsphere for cosmetic injection comprises a microsphere and a water-soluble polysaccharide coating, and the microsphere is one of a hydroxyapatite microsphere, a chitin microsphere, a chitosan microsphere and an acylated chitin / chitosan microsphere. The water-soluble polysaccharide coating is one of carboxyalkyl chitin, carboxyalkyl chitosan, hydroxyalkyl chitin, hydroxyalkyl chitosan, carboxyalkyl cellulose, sodium alginate, potassium alginate, sodium hyaluronate and potassium hyaluronate. The biomass microspheres for cosmetic injection prepared by the invention are used for filling pits, tightening skin, reducing wrinkles, reducing immune response and inflammatory response and improving the compactness and elasticity of the skin.

This invention discloses a chitosan-based lidocaine formulation for intra-articular use and its preparation method. Belonging to the field of pharmaceutical formulation technology, the formulation is prepared from chitosan, carboxymethyl chitosan, lidocaine, chitosan-PNIPAM microspheres, sulfated trehalose, and excipients. The chitosan-PNIPAM used in this invention exhibits dual temperature and pH response. With temperature changes, PNIPAM possesses both hydrophobic contractile and hydrophilic swelling states, thereby controlling the lidocaine release rate. Simultaneously, when the pH decreases, chitosan repels PNIPAM, further disintegrating the microspheres to release the drug, thus achieving targeted analgesia. Sulfated trehalose, as a cartilage matrix synthesis promoter, can activate intracellular signaling pathways in chondrocytes, promoting the synthesis of type II collagen and proteoglycans. Simultaneously, the sulfate groups can form electrostatic complexes with the amino groups of chitosan, enhancing drug retention. The formulation of this invention can inhibit synovial cells, release cytokines, and synergistically with carboxymethyl chitosan, increase the inhibition rate of inflammatory factors by 40%-50%.

The hemostatic gauze for hemodialysis comprises a gauze body, and a plurality of restraint straps are arranged on the gauze body. A contact layer, a hemostasis layer, an isolation layer and a fixing layer are sequentially arranged on the gauze body from bottom to top; the contact layer is made of a hydrophilic biocompatible material; thrombin, chitosan microspheres and gelatin sponge are arranged in the hemostatic layer; the isolating layer is made of a polytetrafluoroethylene film, and the fixing layer is made of elastic medical non-woven fabric. According to the multi-layer structural design that the gauze body is sequentially provided with the contact layer, the hemostasis layer, the isolation layer and the fixing layer from bottom to top, and all the layers are clear in function and cooperate with one another; the contact layer is in direct contact with the wound and is responsible for primary blood coagulation promotion and adhesion prevention; the hemostatic layer can stop bleeding efficiently; the isolating layer plays a dual role in blocking outside bacteria and preventing adhesion; the fixing layer ensures that the whole gauze is stably fixed at the wound part; due to the layered structural design, the hemostatic gauze can effectively meet various requirements for hemostasis and follow-up nursing of a hemodialysis puncture part.

The invention provides propranolol temperature-sensitive hydrogel as well as a preparation method and application thereof, and belongs to the technical field of medicines. The propranolol temperature-sensitive hydrogel disclosed by the invention is prepared from chitosan-beta-sodium glycerophosphate hydrogel and nano microspheres loaded on the chitosan-beta-sodium glycerophosphate hydrogel, wherein the chitosan-beta-sodium glycerophosphate hydrogel is a chitosan-beta-sodium glycerophosphate hydrogel; the nano microspheres are chitosan microspheres which are used for encapsulating propranolol; the mass of the nano microspheres is 1%-20% of the mass of the chitosan-beta-sodium glycerophosphate hydrogel. The propranolol temperature-sensitive hydrogel provided by the invention is a dual slow-release temperature-sensitive hydrogel system, not only has remarkable controlled release performance, can release drugs more continuously and stably, but also has good drug loading property and stable rheological property.

The invention relates to water-soluble CBCS microspheres and preparation and application thereof. Chitosan is dissolved in a solvent to dissolve and swell, and then phthalic anhydride is added for reaction to obtain a reaction solution. The reaction solution is poured into water to cool, the pH value is adjusted to alkaline, and the filtrate is filtered and dialyzed. The filtrate is freeze-dried to obtain flocculent light yellow solid CBCS: N-(2-carboxybenzoyl)-chitosan. The CBCS solution is dripped into an oil phase and an emulsifier for emulsification, and then an aldehyde cross-linking agent is added to cross-link and solidify amino groups and aldehyde groups of the CBCS to generate shiff base bonds to obtain the CBCS microspheres. The CBCS microspheres can adsorb antibiotics in water in accordance with the Langmuir model, and the adsorption process conforms to pseudo-secondary adsorption kinetics. The water solubility and yield of chitosan can be increased, the chitosan microspheres are improved in terms of reusability, and the scope of application can be further broadened. The CBCS microspheres can efficiently, economically, and environmentally friendly treat antibiotic pollution in wastewater.

The invention discloses a method for efficiently extracting bromelain based on magnetic chitosan microspheres, and relates to the technical field of biochemical engineering, and the method comprises the following steps: preparation of magnetic chitosan microspheres, extraction of bromelain, and purification and detection of bromelain. According to the preparation method disclosed by the invention, the magnetic chitosan microspheres with excellent performance are prepared by performing surface modification on the nanoscale ferroferric oxide particles and accurately controlling glutaraldehyde reaction and ultrasonic dispersion, and the adsorption performance is improved by 60%. The microspheres are used for specifically adsorbing bromelain, the extraction efficiency is improved by more than 30% in combination with optimized conditions, the operation time is shortened by 50% through magnetic separation, and the cost is reduced. An adsorption mechanism is defined, conditions are optimized, and the purity of bromelain is improved by 20% or above. A protective agent is added during adsorption and desorption, the retention rate of enzyme activity reaches 90% or above, the product quality and the application value are guaranteed, the application range is expanded, and good application prospects and economic values are achieved.

The application discloses an antibacterial medical packaging material and a preparation method thereof, and relates to the packaging material field. When the antibacterial medical packaging material is prepared, chitosan and N-methylcarbazole-3,6-dialdehyde are reacted to obtain chitosan microspheres; then the chitosan microspheres are sequentially reacted with 2-mercapto-S-thiobenzoyl acetic acid and allyl trimethyl ammonium chloride to obtain modified chitosan microspheres; polylactic acid is reacted with 2-aminoethyl hypophosphorous acid to obtain modified polylactic acid; and the modified chitosan microspheres and the modified polylactic acid are uniformly mixed and then extruded into a film to obtain the antibacterial medical packaging material. The antibacterial medical packaging material prepared by the application has excellent flame retardance, antibacterial property and mechanical property.

The application discloses an intravenous injection immunotargeting microsphere and a preparation method and application thereof, and relates to the field of lung tissue engineering.The method steps of the application comprise the following steps: preparing a drug-loaded chitosan solution; preparing the drug-loaded chitosan microsphere by using a water-in-oil emulsion method; grinding the drug-loaded microsphere in a mortar; then, the drug-loaded chitosan microsphere is soaked in a dopamine solution to react for a period of time to construct a dopamine active coating; finally, the abundant amino and hydroxyl functional groups of the chitosan microsphere are combined with an immunotargeting factor iNOS inhibitor through electrostatic interaction.The intravenous injection immunotargeting microsphere prepared in the application has good biocompatibility and size stability, can specifically recognize target cells and realize precise drug delivery, effectively improves the treatment efficiency of acute lung injury, and has good clinical application potential.

The invention provides cod liver oil pure chitosan microspheres as well as a preparation method and application thereof, and belongs to the technical field of chitosan microspheres. The method comprises the following steps: uniformly stirring cod liver oil and a chitosan solution according to a volume ratio of 1: (2-6) to obtain an emulsion m1; extruding the emulsion m1 into a sodium dodecyl sulfate solution to obtain cod liver oil chitosan microspheres ms2; drying the cod-liver oil chitosan microspheres ms2, and putting the cod-liver oil chitosan microspheres ms2 into a sodium dodecyl sulfate solution for standing to obtain cod-liver oil chitosan microspheres ms3; immersing the cod-liver oil chitosan microspheres ms3 into a sodium hydroxide solution, and then washing the cod-liver oil chitosan microspheres ms3 to be neutral to obtain cod-liver oil chitosan microspheres ms4. Through a self-assembly technology at a mild temperature, a cross-linking agent does not need to be used, the preparation process is simple, and the obtained cod-liver oil pure chitosan microspheres are high in biological safety, good in antibacterial activity and high in embedding rate. The invention also provides application of the cod-liver oil pure chitosan microspheres in preparation of foods and medicines, and the cod-liver oil pure chitosan microspheres have a good application prospect.

The present application relates to a kind of active wound dressings based on bacterial cellulose and chitosan microspheres encapsulating drugs and its preparation method, the chitosan microspheres utilize bacterial cellulose nanometer network as dispersed phase, let chitosan be dispersed in bacterial cellulose matrix into ball by ionic crosslinking method, and drug is encapsulated in ball, microsphere is attached on the nanometer fiber inside bacterial cellulose film or network inside pore.The active wound dressings of the present application has broad-spectrum antibacterial property and biological safety;Method is simple and efficient and avoid the use of toxic crosslinking agent, can make chitosan microspheres evenly distributed in film.When the encapsulated drug is berberine, it has the potential to treat diabetic foot.

The invention relates to the technical field of preparation of natural antibacterial neutral agents, and discloses a preparation method of a natural antibacterial neutral agent based on a PLGA slow release mechanism, which comprises the following steps: taking polylactic acid PLGA as a carrier, mixing meloxicam polylactic acid microspheres, a chitosan solution and a cross-linking agent solution, adding a surfactant, and stirring to form a uniform emulsion; adding the emulsion into a hardening agent, and stirring until the cross-linking reaction is completed to obtain chitosan microspheres; the chitosan microspheres are washed with deionized water, and the surfactant and the cross-linking agent remaining on the surfaces are removed; and drying the chitosan microspheres in a ventilation drying chamber to obtain the natural antibacterial neutral agent based on the PLGA slow release mechanism. The meloxicam polylactic acid microspheres are prepared by taking PLGA as a carrier, so that the slow release effect of the medicine can be realized. The sustained release of the drug on the skin surface is facilitated, so that the balance of the pH value of the skin is maintained for a longer time, and the repair of the skin barrier and the maintenance of the microbial balance are promoted.