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NGF (nerve growth factor) chitosan microsphere and high-bionic stent slow releasing system and preparation method thereof

A technology of chitosan microspheres and chitosan, which is applied in the field of nerve tissue engineering scaffolds, can solve the problems of inability to release nerve growth factor effectively for a long time, difficulty in nerve regeneration, and large difference, so as to protect biological activity and promote Restoration, the effect of broad application prospects

Active Publication Date: 2012-07-25
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a slow-release system of NGF chitosan microspheres-high biomimetic scaffold, which solves the problem that the internal ultrastructure of the existing nerve tissue engineering scaffold system is quite different from the normal nerve basement membrane structure, and it is difficult to guide better. Nerve regeneration, and the inability to effectively release nerve growth factors for a long time to promote nerve regeneration better and faster

Method used

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  • NGF (nerve growth factor) chitosan microsphere and high-bionic stent slow releasing system and preparation method thereof
  • NGF (nerve growth factor) chitosan microsphere and high-bionic stent slow releasing system and preparation method thereof
  • NGF (nerve growth factor) chitosan microsphere and high-bionic stent slow releasing system and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0131] At a low temperature of 2°C, first dissolve 40 μg of nerve growth factor in 10 ml of PBS solution with a pH of 7.2, then pour 800 μg of bovine serum albumin into the solution and completely dissolve to prepare a nerve growth factor solution; 700mg chitosan powder is completely dissolved in 70ml mass-volume concentration of 3mg / ml acetic acid aqueous solution; take 7ml nerve growth factor solution and 35ml chitosan acetic acid solution and fully mix to prepare the water phase; mix 140ml liquid paraffin oil with 2ml surface After the active agent Span80 is measured, it is poured into the three-necked flask in turn, and mechanically stirred for 3 minutes at a stirring speed of 300r / min, so that the liquid paraffin oil and the surfactant Span80 are evenly mixed to prepare an oil phase; at a low temperature of 2°C , add 20ml of the aqueous phase solution dropwise to 100ml of the oil phase, use mechanical stirring for 0.5h, and the stirring speed is 800r / min, to prepare water / ...

Embodiment 2

[0133] At a low temperature of 4°C, first dissolve 60 μg of nerve growth factor in 10 ml of PBS solution with a pH of 7.4, then pour 1500 μg of bovine serum albumin into the solution and completely dissolve to prepare a nerve growth factor solution; 1500mg chitosan powder is completely dissolved in 100ml mass-volume concentration of 3mg / ml acetic acid aqueous solution; fully mix 10ml nerve growth factor solution with 100ml chitosan acetic acid aqueous solution to prepare the water phase; mix 300ml liquid paraffin oil with 4ml surface After the amount of active agent Span80 is taken, it is poured into the three-necked flask in turn, and mechanically stirred for 5 minutes at a stirring speed of 500r / min, so that the liquid paraffin oil and the surfactant Span80 are evenly mixed to prepare an oil phase; at a low temperature of 4°C , add 30ml of water phase solution dropwise to 300ml of oil phase, use mechanical stirring for 1h, stirring speed is 1000r / min, prepare water / oil emulsi...

Embodiment 3

[0135] At a low temperature of 6°C, dissolve 160 μg of nerve growth factor in 20 ml of PBS solution with a pH of 7.6, and then pour 4800 μg of bovine serum albumin into the solution to completely dissolve to prepare a nerve growth factor solution; 4000mg of chitosan powder is completely dissolved in 200ml of acetic acid solution; 10ml of nerve growth factor solution is fully mixed with 150ml of chitosan solution to prepare an aqueous phase; after measuring 240ml of liquid paraffin oil and 3ml of surfactant Span80, Sequentially pour into the three-necked flask, mechanically stir for 7 minutes, and the stirring speed is 700r / min, so that the liquid paraffin oil and the surfactant Span80 are evenly mixed to prepare the oil phase; Add the mixed solution of chitosan and 240ml dropwise into the oil phase, adopt mechanical stirring for 1.5h, and the stirring speed is 1200r / min, to prepare water / oil emulsion; The solution was added dropwise to the water / oil emulsion and mechanically s...

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Abstract

The invention discloses an NGF (nerve growth factor) chitosan microsphere and high-bionic stent slow releasing system, which is a cylindrical collagen and chitosan nerve stent. Chitosan microspheres buried with nerve growth factors are uniformly loaded in the stent. A preparation method of the stent includes firstly, preparing the chitosan microspheres buried with the nerve growth factors by an emulsification chemical-crosslink method; secondly, preparing the collagen and chitosan nerve stent with mutually parallel axial microtubules sample structures; and finally, compounding the chitosan microspheres onto the collagen and chitosan nerve stent via after-compounding technology and preparing the NGF chitosan microsphere and high-bionic stent slow releasing system. The stent structure is similar to a normal nerve basilar membrane structure, and the stent realizes an effect of physically guiding regeneration nerves to the greatest extent. Besides, the stent can slowly release the nerve growth factors with bioactivity for 12 weeks, can lead the nerve growth factors to be effectively released to a nerve injury portion for a long time, and accordingly achieves the purpose of promoting long nerve defect repair better and faster.

Description

technical field [0001] The invention belongs to the technical field of nerve tissue engineering scaffolds, and relates to a nerve tissue engineering scaffold, in particular to an NGF chitosan microsphere-high biomimetic scaffold slow-release system, and also relates to a preparation method of the slow-release system. Background technique [0002] Nerve regeneration and functional recovery after peripheral nerve injury is a difficult problem in clinical treatment. Autologous nerve transplantation is currently the preferred method of nerve reconstruction (“gold standard”) in clinical practice, but it is greatly limited by sensory dysfunction at the donor site, limited donor sources, mismatch in size and type of nerve donor and recipient, and prolonged operation time. Application; allogeneic nerve regeneration pipeline refers to endoneurial tubes that have been cleared of Schwann cells and other biomacromolecules to create a good environment for nerve regeneration. This pipelin...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/22A61L27/20A61L27/54A61L27/50B01J13/04A61F2/02
Inventor 罗卓荆曾文黄景辉胡学昱
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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