Chitin tetra ammonium salt nano-particle, its preparation method and use

A technology of chitosan quaternary ammonium salt and nano-particles, which is applied in pharmaceutical formulations, medical preparations of non-active ingredients, powder delivery, etc., and can solve problems such as inability to apply sensitive macromolecules, failure to meet requirements well, and reduction , to achieve high encapsulation rate, meet environmental protection requirements, and good slow-release effect

Inactive Publication Date: 2005-10-26
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chitosan is insoluble in water when the pH is above 6, making it unsuitable for adsorbing ions under neutral conditions, and preparing nanoparticles under neutral conditions can avoid the side effects of organic solvents, and the energy required is also higher than ordinary The preparation method (solvent evaporation and emulsification-solvent diffusion method) is greatly reduced, so chitosan can no longer meet the requirements as a drug carrier, especially for the delivery of sensitive macromolecules such as protein drugs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: making concentration of chitosan quaternary ammonium salt is 1.6mg / ml aqueous solution, then model drug bovine serum albumin is dissolved in chitosan quaternary ammonium salt solution, bovine serum albumin concentration is 1.5mg / ml; Then Add 2ml of 0.5mg / ml sodium tripolyphosphate aqueous solution into 5ml of chitosan quaternary ammonium salt solution, and generate nanoparticles under stirring conditions at room temperature. The encapsulation efficiency of nanoparticles to bovine serum albumin reaches 77%, and in normal saline, the cumulative sustained release reaches 52% in six days.

Embodiment 2

[0013] Embodiment 2: the chitosan quaternary ammonium salt is made into a concentration of 1.0mg / ml aqueous solution, and then the model drug bovine serum albumin is dissolved in the chitosan quaternary ammonium salt solution, and the bovine serum albumin concentration is 0.8mg / ml; then Add 2ml of sodium tripolyphosphate aqueous solution with a concentration of 0.8mg / ml into 5ml of chitosan quaternary ammonium salt solution, and generate nanoparticles under stirring conditions at room temperature. The encapsulation efficiency of nanoparticles to bovine serum albumin is up to 89%, and in normal saline, the accumulated slow release reaches 29% in six days.

Embodiment 3

[0014] Embodiment 3: making concentration of chitosan quaternary ammonium salt is an aqueous solution of 1.6mg / ml, then the model drug bovine serum albumin is dissolved in chitosan quaternary ammonium salt solution, bovine serum albumin concentration is 1.0mg / ml; then Sodium alginate (concentration of sodium alginate is 0.5mg / ml) and sodium tripolyphosphate (concentration of sodium tripolyphosphate is 0.3mg / ml) aqueous solution that is dissolved in 2ml is added in the chitosan quaternary ammonium salt solution of 5ml, Nanoparticles were generated under stirring conditions at room temperature. The encapsulation efficiency of nanoparticles to bovine serum albumin reaches 17.5%, and in normal saline, the cumulative sustained release reaches 32% in six days.

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Abstract

A chitosen-quaternary ammonium salt nanoparticle as the carrier of protein-type medicine is prepared through dissolving chitosan-quaternary ammonium salt in distilled water, stirring while adding solution of sodium tripolyphosphate, cross-linking, centrifugal separation, and freeze drying. It has long relesing period (6 days).

Description

technical field [0001] The invention relates to a chitosan quaternary ammonium salt nano particle used as a drug carrier, a preparation method and application thereof. Background technique [0002] Chitosan is widely used in drug carriers and medical auxiliary materials because of its advantages of high adhesion, good biocompatibility, non-toxicity and no side effects. However, chitosan is insoluble in water when the pH is above 6, making it unsuitable for adsorbing ions under neutral conditions, and preparing nanoparticles under neutral conditions can avoid the side effects of organic solvents, and the energy required is also higher than ordinary The preparation methods (solvent evaporation and emulsification-solvent diffusion method) are greatly reduced, so chitosan can no longer meet the requirements as a drug carrier, especially for the delivery of sensitive macromolecules such as protein drugs. Contents of the invention [0003] In order to overcome the deficiencies ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/36
Inventor 杜予民徐咏梅
Owner WUHAN UNIV
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