150results about How to "High encapsulation efficiency" patented technology

The invention relates to a targeted drug delivery carrier based on mesoporous polydopamine nanoparticles and application thereof.The targeted drug delivery carrier is galactose modified mesoporous dopamine coordinated with manganese ions, lysine is adopted in the carrier synthesis step to provide an alkaline environment needed by dopamine polymerization, the polymerization process is simplified, and the drug delivery efficiency is improved. Meanwhile, lysine can be introduced into the mesoporous polydopamine; and the drug loading capacity of the carrier is improved. The carrier gathers multifunctionality of drug delivery, tumor imaging and treatment, provides a comprehensive new method for tumor treatment, and enhances individuation and precision of treatment.

The invention belongs to the field of biological medicines, and relates to a drug-loaded nano-vesicle as well as a preparation method and application thereof. The drug-loaded nano-vesicle comprises a vesicle core and a drug-loaded nano-vesicle, wherein the vesicle core comprises siRNA (small interfering Ribonucleic Acid) capable of specifically targeting and silencing an NR1D1 gene; the vesicle membrane is formed by fusing an erythrocyte membrane, a macrophage membrane, cardiolipin, cholesterol and lecithin. The drug-loaded nano-vesicle can specifically target macrophages in a sepsis immunosuppression stage, has an intracellular response release function, recovers BMAL1 and IGF2BP2-ATP6V1B2 / ATP6V0c axis functions by inhibiting NR1D1 expression, reconstructs a macrophage phagocytosis function and lysosome-dependent bacterium removal capability, and can be used for preparing a drug-loaded nano-vesicle with a specific targeting function. The survival rate of sepsis immunosuppression model animals is obviously improved; and the bacterial load is reduced. Compared with a traditional electroporation method, the preparation method disclosed by the invention has the advantage that the encapsulation efficiency of siRNA is remarkably improved.

The invention discloses glabridin liposome, a whitening product, a preparation method and application, and relates to the technical field of cosmetics. The glabridin liposome is prepared from the following components in percentage by mass: 0.5%-2.5% of glabridin, 1%-8% of phospholipid, 0%-1% of cholesterol, 10%-15% of liquid grease, 0.5%-1.5% of an emulsifier, 0.5%-1.5% of an antioxidant, 0.2%-1% of trehalose, 10%-30% of glycerol and the balance of water. Through innovation of the process and formula, the problems of instability of glabridin, non-uniformity of lipidosome and the like are solved, the encapsulation efficiency can be improved, the transdermal absorption capacity and stability can be improved, and the inhibitory activity on cell tyrosinase and melanin can be improved.

The invention discloses a nitrogen-containing chain compound, a preparation method, a composition containing the nitrogen-containing chain compound and application, and particularly provides the nitrogen-containing chain compound as shown in the formula I or pharmaceutically acceptable salt of the nitrogen-containing chain compound. An LNP preparation prepared from the nitrogen-containing chain compound is relatively uniform in nanoparticle size, high in encapsulation efficiency and good in stability. The in-vivo expression activity is relatively high. # imgabs0 #

The invention provides a camptothecin prodrug. The camptothecin prodrug provided by the invention has higher drug retention in the liposome and better stability, and the prodrug liposome can maintain long-time plasma concentration in plasma. In-vitro characterization of the camptothecin prodrug liposome shows that the camptothecin prodrug liposome has higher encapsulation efficiency, drug loading capacity and stability, and can achieve a slow release effect in plasma; the experimental result on the cellular level shows that the compound has higher cellular uptake rate; in-vivo experiments prove that the compound has a better tumor accumulation effect and antitumor activity, can tolerate a higher dosage, and has higher biological safety.

The invention discloses a preparation method of a squalene and tocopherol composition microcapsule, and relates to the technical field of microcapsule preparation, and the preparation method is characterized by comprising the following steps: squalene, tocopherol and an emulsifier form a core material emulsion, Arabic gum, modified corn starch and maltodextrin are compounded to obtain a wall material emulsion, and the wall material emulsion is prepared into the squalene and tocopherol composition microcapsule. Uniformly mixing the core material emulsion and the wall material emulsion, and performing a spray drying process to obtain the squalene and tocopherol composition microcapsule. The obtained microcapsule has good thermal stability and antioxidant activity, and can well protect active components.

The invention belongs to the field of pharmaceutical preparations, and particularly relates to a zomib nano-micelle preparation and a preparation method thereof. The carfilzomib nano-micelle preparation comprises carfilzomib, a micelle carrier material and a stabilizer, wherein the mass ratio of the carfilzomib to the micelle carrier material to the stabilizer is 1: (15-30): (15-30); and the carrier material is PEG (Polyethylene Glycol)-DSPE (Distearoyl PEG-DSPE is used as a drug carrier to prepare the carfilzomib micelle preparation, the particle size is 8-20 nm, the encapsulation efficiency is high, and the carfilzomib micelle preparation has a very strong cell penetration effect and cannot be quickly cleared. Meanwhile, the preparation operation is simple, and freeze-drying operation is avoided. Compared with the existing carfilzomib preparation, the toxicity is reduced under the same dosage.

The invention provides novel ionizable lipid as well as a preparation method and application thereof. The structural formula is shown as a formula (I). The ionizable lipid compound contains urea and a bioisostere structure thereof, the encapsulation effect of the ionizable lipid is improved through hydrogen bonds formed by squaramide, urea and thiourea structures and nucleic acid, and the immunogenicity of the ionizable lipid is reduced. The nanoparticles prepared from the ionizable lipid are high in encapsulation efficiency, low in PDI and wide and stable in process adaptability. # imgabs0 #

The invention discloses an Escherichia coli phage microcapsule causing sheep diarrhea and a preparation method of the Escherichia coli phage microcapsule. The preparation method comprises the following steps: by taking sheep-derived pathogenic escherichia coli as host bacteria, separating from an environmental sewage sample to obtain bacteriophage, and carrying out microencapsulation on the bacteriophage to obtain the bacteriophage microcapsule. Experiments show that the bacteriophage microcapsule has a relatively good treatment effect on sheep-derived pathogenic escherichia coli infection, has relatively strong tolerance and release property to a digestion environment, and has a good application prospect in the aspects of research and development of oral bacteriophage preparations and the like.

The invention discloses a natto peptide sea cucumber polysaccharide liposome for synergistically improving cardiovascular health and a preparation method and application thereof, belongs to the field of active peptide delivery systems, and aims to solve the stability problem of natto oligopeptide in gastrointestinal digestive tracts. The phytosterol and the vitamin E are filled between phospholipid double layers of the liposome to support a gap between the phospholipid double layers, and the ratio of the phytosterol to the vitamin E is (1-3): 1, so that the natto oligopeptide liposome has the effects that the storage of the natto oligopeptide and the stability of the natto oligopeptide in gastrointestinal and digestive tracts can be improved, the bioavailability of the natto oligopeptide liposome is improved, and the bioavailability of the natto oligopeptide liposome is improved. The traditional Chinese medicine composition has synergistic effects in the aspects of anticoagulation, thrombolysis and blood fat reduction, and has a good cardiovascular protection effect.

The invention discloses a lutein-loaded functional hollow salt and a preparation method thereof, the functional hollow salt is prepared by taking a Maillard reaction product of whey protein isolate and maltodextrin as a salt carrier, mixing the salt carrier with lutein-loaded vegetable oil and carrying out spray drying, the weight percentage of sodium chloride in the functional hollow salt is greater than 50% and less than or equal to 85%. The particle size of the hollow salt is 1 / 10 of that of common salt. The reduction of the particle size generally shows that a denser particle structure is formed in the spray drying process, so that the exposure of active ingredients can be reduced, the possibility of oxidation or photolysis is further reduced, and the stability of the ingredients is improved; through comparison of sensory evaluation, it is found that when the same salty taste feeling is achieved, 20-30% of salt can be reduced in the hollow salt; the lutein-loaded hollow salt prepared by the invention is relatively dispersed, has no adhesion phenomenon, and can be stored for a long time under a certain condition.

The invention discloses a preparation method of waxberry anthocyanin lipidosome, and belongs to the field of waxberry anthocyanin lipidosome. The preparation method of the waxberry anthocyanin lipidosome comprises the following steps: dissolving anthocyanin, cholesterol and soybean lecithin in an organic solvent, and stirring in an ultrasonic crusher to obtain a mixture; and stirring the mixture, transferring the mixture into a pear-shaped bottle, carrying out rotary evaporation, hydrating, and filtering to obtain the waxberry anthocyanin liposome. The waxberry anthocyanin liposome prepared by the method has better stability; moreover, after the preparation conditions of the waxberry anthocyanin liposome are optimized by using a response surface method, the obtained waxberry anthocyanin liposome has relatively high encapsulation efficiency, so that a theoretical basis is provided for development of a novel anthocyanin dosage form and application of the novel anthocyanin dosage form in a health-care food market.

The invention discloses a temperature-adjusting textile based on phase-change microcapsules and a preparation method thereof, and belongs to the technical field of textiles, the method comprises the following steps: (1) preparing a polymer solution into nanofibers by using an electrostatic spinning method, and weaving the nanofibers and natural fiber blended yarns to obtain a basic fabric; (2) heating and melting paraffin, adding 3-methacrylamide dopamine and methacrylic acid, finally adding an initiator to prepare an oil-phase solution, pouring the oil-phase solution into a water-phase solution containing an emulsifier, stirring and emulsifying, and heating to generate polymerization reaction to obtain a phase change microcapsule; and (3) soaking the basic fabric in a solution containing the phase-change microcapsules to enable the phase-change microcapsules to be self-adhered to the basic fabric, taking out the basic fabric, padding, and drying to obtain the temperature-adjusting textile based on the phase-change microcapsules. The method is simple in steps, the phase change microcapsules are high in adhesive force on the basic fabric, and the obtained temperature adjusting textile has excellent heat storage and temperature adjusting functions.

The invention belongs to the technical field of medicines, and relates to a cholesterolized rapamycin liposome as well as preparation and application thereof. The invention relates to a cholesterylated rapamycin liposome, which is prepared from cholesterylated rapamycin, phospholipid and a phospholipid PEG (Polyethylene Glycol) derivative. The liposome is mainly composed of a rapamycin cholesterol conjugate, the coupling effect of cholesterol and rapamycin indirectly enables a drug and phospholipid to be tightly combined, compared with free rapamycin, the entrapment efficiency is greatly improved, the leakage rate is reduced (the entrapment efficiency is improved by 8.05%, and the leakage rate is reduced by 100%), the entrapment efficiency of the preparation is as high as 95% or above, and the preparation has good application prospects. The particle size is about 120nm, the PDI is less than 0.2, the drug can be stably delivered in vivo, the circulation time of the drug in vivo is prolonged, the toxic and side effects are reduced, the safety is improved, the stability is good, and the storage, transportation and use are facilitated.

The invention discloses a ferroptosis-inducing targeted mPDA / MSNs nano-drug delivery carrier as well as a preparation method and application thereof, and relates to the technical field of preparation of targeted nano-delivery carriers. The preparation method comprises the following steps: firstly, connecting and modifying-NH2 to the surfaces of MSNs to obtain amino modified MSNs-NH2; the preparation method comprises the following steps: coating with mPDA to obtain mPDA / MSNs-NH2, and reacting with ferrous lactate to obtain Fe < 2 + >-mPDA / MSNs-NH2; the final carrier RGD-Fe2 +-mPDA / MSNs-NH2 is prepared by coupling an RGD targeting peptide, the particle size is uniform, the physicochemical property is stable, the ATRA and DOX encapsulation efficiency and the drug loading capacity are high, the drug can be responsively released in a simulated tumor microenvironment, a drug-loaded sample RFP / MN atD / A can deliver a drug into tumor cells in a targeting manner, ferroptosis can be rapidly induced, and the tumor cells can be effectively killed through synergistic chemotherapy.

The invention belongs to the field of medical biology, and relates to a medical preparation, in particular to a nano supramolecular drug-loaded liposome and a preparation method thereof. The nano supramolecular drug-loaded liposome is prepared from the following raw materials in percentage by weight: 1 to 20 percent of active ingredient, 5 to 15 percent of lecithin, 20 to 40 percent of eutectic solvent, 0.1 to 10 percent of grease, 0.1 to 0.5 percent of cholesterol, 0.1 to 5 percent of emulsifier, 0.1 to 1.5 percent of stabilizer and the balance of deionized water, and the active ingredient is water-soluble VC (prototype VC). According to the invention, the active ingredients and the deep eutectic solvent are subjected to ultrasonic treatment to form a ternary supramolecular VC structure, and then the ternary supramolecular VC structure is wrapped, so that the stability of VC molecules in a solution state is greatly improved by virtue of dual clamping of supramolecules and lipidosome, the problem of oxidation discoloration of the VC molecules in a long-term storage process is improved, and the effect of VC in the cosmetic industry is better played.

The invention relates to the technical field of sodium sarcosine, in particular to slow-release sodium sarcosine and a preparation method thereof. The slow-release sodium sarcosine is prepared from the following raw materials in parts by weight: 70 to 80 parts of sodium sarcosine aqueous solution, 5 to 10 parts of acrylic acid, 5 to 10 parts of acrylamide, 3 to 5 parts of hydrotropy monomer A, 0.5 to 1 part of hydrotropy monomer B, 0.05 to 0.15 part of cross-linking agent and 0.05 to 0.15 part of initiator, and the hydrotropy monomer B is a mixture of diallyl dimethyl ammonium chloride and methacryloyloxyethyl trimethyl ammonium chloride. The sustained-release sodium sarcosinate disclosed by the invention has relatively high drug loading capacity and encapsulation efficiency, does not generate a burst release phenomenon, and can act for a relatively long time in vivo, so that the benefits of the sustained-release sodium sarcosinate are better exerted, the intake frequency is reduced, and the sustained-release sodium sarcosinate has a wide application prospect.

The application belongs to the technical field of biological medicine, and particularly relates to a kind of lipid nanoparticles and engineered immune cells. The application first provides an engineered immune cell, the engineered immune cell includes immune cell, lipid nanoparticle and exogenous drug;The exogenous drug is loaded in the lipid nanoparticle;When the immune cell is contacted with the lipid nanoparticle, the immune cell and the lipid nanoparticle endocytosis occur to obtain the engineered immune cell. In another aspect, the application also provides a kind of lipid nanoparticles that can improve the encapsulation efficiency of mRNA and the delivery efficiency of immune cells.

An object of present invention is to provide a compound or a salt thereof constituting lipid particles that can achieve a high nucleic acid encapsulation rate and excellent delivery of nucleic acids, and to provide lipid particles and pharmaceutical composition using the compound or a salt thereof, which can achieve a high nucleic acid encapsulation rate and excellent delivery of nucleic acids. According to an aspect of the present invention, a compound represented by Formula (1) or a salt thereof is provided. In the formula, each symbol has a meaning defined in the present specification.

The invention provides a butylphthalide injection and a preparation method thereof, and relates to the technical field of medicines.The butylphthalide injection comprises butylphthalide, cholate, phospholipid and water for injection, the cholate is prepared through a reaction of cholic acid and sodium hydroxide; the molar ratio of the phospholipid to the cholic acid is (0.3-1.2): 1. The mass fraction of butylphthalide in the butylphthalide injection is 0.025%-1%, the butylphthalide, cholate and phospholipid are mixed and proportioned under the conditions of certain concentration and mass ratio and cholic acid and phospholipid in the cholate under the condition of certain molar ratio, and the butylphthalide injection of a clear solution is prepared; the preparation process of the butylphthalide injection is simple, the stability of the injection is further improved through repeated dissolving and stirring, clinical medication requirements are met, and industrial production is facilitated.

The invention provides a silicon dioxide essence microcapsule which is characterized in that essence is coated in a hydrophobic organic compound in a mononuclear or multinuclear form to form a core layer; and silicon dioxide is a shell layer. The outer layer of the microcapsule is a silicon dioxide shell, the secondary outer layer is a hydrophobic organic compound which is solid at room temperature, and essence is dispersed in the secondary outer layer in a single-core or multi-core form. The preparation method adopts a soap-free method and comprises the following steps: mixing and emulsifying essence, an organic compound which is in a solid state at room temperature and a polyalkoxy siloxane precursor, and then performing hydrolytic condensation on the precursor on a water phase interface to form a silicon dioxide shell so as to realize coating. The method is environment-friendly, and a surfactant does not need to be added. The obtained microcapsule has the characteristics of high encapsulation efficiency, excellent long-acting slow release performance, excellent thermal response controlled release capability and capability of desorbing and reloading essence after being used, and has wide application prospects in the fields of daily chemicals, textiles, foods and the like.

The invention belongs to the technical field of algal polysaccharide extraction, and discloses a modification extraction method of algal asparagus polysaccharide and a method for preparing a lutein coating by using the algal asparagus polysaccharide. The modified extraction method comprises the following steps: extracting gracilaria lemaneiformis powder with a weak base solution, enabling lactide and polysaccharide to be subjected to grafting reaction through a redox initiation system under the assistance of ultrasonic waves, and then separating out a first polysaccharide component with high emulsibility and a second polysaccharide component with high gel property through graded alcohol precipitation. The two obtained functional components are used for coating xanthophyll, specifically, the first polysaccharide component emulsifies a xanthophyll oil phase to form a pre-emulsion, the second polysaccharide component is compounded with gelatin, a composite coacervate is formed on an oil drop interface by adjusting the pH value, and finally drying and curing are conducted. According to the method, two modified polysaccharides with definite functions can be synchronously obtained, the prepared xanthophyll coating is high in encapsulation efficiency, good in storage and processing stability and excellent in water dispersibility, and the problems that xanthophyll is unstable and difficult to apply are effectively solved.

The invention discloses a preparation method of griseofulvin eye drops, and belongs to the technical field of pharmaceutical preparations. Hemoglobin is dissolved in water as an aqueous phase, and griseofulvin is dissolved in an organic solvent as an organic phase; the organic phase is added to the aqueous phase, and the organic solvent is removed after stirring; dopamine hydrochloride is then added, and then a Tris buffer solution is added under stirring conditions and continued to be stirred; finally, a cell-penetrating peptide is added and stirred to react to obtain griseofulvin eye drops. The cell-penetrating peptide is used to penetrate the eye barrier and is combined with the nanostructure formed by hemoglobin, griseofulvin and dopamine. The nanopreparation can carry drugs to the posterior segment of the eye by local eye drops.

The invention relates to the field of ginsenoside liposome extraction, in particular to a preparation method of rare ginsenoside liposome. According to the preparation method of the rare ginsenoside lipidosome, the problem that rare ginsenoside is not dispersed in water and insoluble in water is solved, the vacuum freezing heating drying machine is further provided, ice crystals are sequentially fed into the vacuum heating drying system in a low-flow-density state through the spray freezing system, and the rare ginsenoside lipidosome is obtained. According to the present invention, the particulate powder containing the ginsenoside liposome is rapidly dried, and the smaller the particle size of the particulate powder containing the ginsenoside liposome is, the easier the ginsenoside liposome is absorbed by the human body, such that the bioavailability of the conventional rare ginsenoside absorbed by the human body is improved, and the rare ginsenoside liposome with characteristics of high entrapment rate and high drug loading capacity can be continuously produced, finally, the technical defect that the human body absorption bioavailability of the ginsenoside liposome is relatively low due to the problems of low encapsulation efficiency and low drug loading capacity after the extracted ginsenoside liposome is processed into a drug product is overcome.

The present invention provides a polypolypeptide gene vector and a preparation method and application thereof, wherein the polypolypeptide gene vector comprises nanospheres formed by self-assembly of triblock polypolypeptides, wherein the triblock polypolypeptides comprise polyethylene glycol, polylysine and polyleucine connected in sequence; one end of the polylysine is connected to the polyethylene glycol via an amide bond, and the other end of the polylysine is connected to the polyleucine via a peptide bond. The polypolypeptide gene vector of the present invention can efficiently load the target gene in the polypolypeptide gene vector, and can form stable gene particles with high encapsulation rate and high load; the obtained polypolypeptide gene vector can produce a protonated sponge effect inside the lysosome to promote the lysosomal escape of the target gene and prevent the target gene from being degraded in the lysosome. It has important application prospects in the fields of gene editing, gene diagnosis, gene therapy and gene vaccines.

The invention provides a spirotetramat polyurethane / polyurea microcapsule suspending agent as well as a preparation method and application thereof, and belongs to the technical field of pesticide preparations. The preparation method comprises the following steps: mixing an oil-phase solution containing spirotetramat raw medicine and polyisocyanate with a water-phase solution containing an emulsifier and a dispersant, carrying out shear emulsification, adding a water-soluble wall material monomer water solution containing polyamine and / or polyol, and carrying out an interfacial polymerization reaction; after the polymerization is finished, sequentially carrying out curing treatment and cooling, then adding an auxiliary agent, and blending to obtain the spirotetramat polyurethane / polyurea microcapsule suspending agent. The spirotetramat microcapsule suspending agent is prepared through an interfacial polymerization method, spirotetramat is coated in a polyurethane or polyurea capsule wall, and the spirotetramat microcapsule suspending agent has the advantages of being high in encapsulation efficiency, good in physical and chemical stability, long in pesticide effect lasting period, safer to non-target organisms (such as bees) and the environment and the like. The preparation method is stable in process, easy for industrial production and excellent in product performance.

This invention discloses a compound preparation for treating vascular dementia and its preparation method. The active pharmaceutical ingredient consists of protopanaxadiol and gallic acid in a 1:1 mass ratio, and the dosage form is liposomes. The preparation method includes extraction, mixing, liposome preparation, and optional freeze-drying steps: protopanaxadiol and gallic acid are extracted and purified from ginseng rhizome and Terminalia chebula fruit, respectively. After mixing, they are combined with phospholipids and cholesterol to prepare a lipid membrane. The membrane is then homogenized by hydration, low-temperature ultrasound, and high-pressure microfluidic jet to obtain a homogeneous liposome suspension, which can be further freeze-dried into a freeze-dried powder. The 1:1 composition of protopanaxadiol and gallic acid targets multiple points on the pathological mechanism of vascular dementia, showing significant therapeutic effects and broad application prospects. This preparation uses a liposome dosage form to improve the bioavailability of the active ingredient, the preparation process parameters are controllable, the active ingredient has high purity, the liposomes have uniform particle size, high encapsulation efficiency, and good stability.

The present invention belongs to the field of pharmaceutical preparations, and relates to a nano preparation for treating colitis, and a preparation method and application thereof. The components of the nano preparation include: piperine, glycyrrhizic acid, a stabilizer, a colon targeting material, and a pore-forming agent. The particle size of the nano preparation is 100-300 nm, the encapsulation rate of piperine and glycyrrhizic acid is 80.5-99.5%, and the drug loading is 20.5-60.5%. The nano preparation can achieve colon-specific release, increase the synchronous release of piperine and glycyrrhizic acid in the colon, synergistically enhance the anti-colitis effect, and significantly improve the anti-inflammatory effect compared with a single drug and a common nano preparation. In addition, the nano preparation can be used as an intermediate to prepare oral granules, capsules, tablets, etc., for clinical effective treatment of colitis.

The invention provides a flurbiprofen axetil injection and a preparation method thereof, and belongs to the technical field of biological medicines. According to the flurbiprofen axetil injection provided by the invention, oleic acid is used as a pH regulator to control the pH value of an injection preparation, hydrolysis of soybean oil and egg yolk lecithin can be inhibited, and egg yolk phosphatidyl glycerol (EPG) is added as a co-emulsifier, so that the encapsulation efficiency of an emulsion is remarkably improved, the quantity of flurbiprofen axetil free in water is reduced, and the stability of the injection is improved. The later-stage hydrolysis is obviously reduced. Therefore, the content of the flurbiprofen axetil injection provided by the invention is relatively stable, meanwhile, the quantity of the hydrolysate flurbiprofen is small, the relatively stable pH value of the preparation is ensured, the clinical safety and effectiveness of the preparation are ensured, and the validity period is not less than 2 years.

The application discloses black soldier fly oil freeze-dried liposomes and a preparation method thereof. The preparation method of the black soldier fly oil freeze-dried liposomes comprises the following steps: dissolving phospholipid, sterol, black soldier fly oil and a surfactant in an organic solvent to form a film, hydrating the film with a buffer solution, and preparing nano-liposomes through pulse ice bath ultrasonic treatment; adding a freeze-drying protective agent, uniformly mixing, pre-freezing, and freeze-drying, so as to obtain the black soldier fly oil freeze-dried liposomes. The stability of the black soldier fly oil is remarkably improved through the freeze-drying process, long-term storage of the liposomes at room temperature is realized, and a new strategy is provided for the development of a functional oil delivery system.