36results about How to "High encapsulation efficiency" patented technology

The invention belongs to the field of biological medicines, and relates to a drug-loaded nano-vesicle as well as a preparation method and application thereof. The drug-loaded nano-vesicle comprises a vesicle core and a drug-loaded nano-vesicle, wherein the vesicle core comprises siRNA (small interfering Ribonucleic Acid) capable of specifically targeting and silencing an NR1D1 gene; the vesicle membrane is formed by fusing an erythrocyte membrane, a macrophage membrane, cardiolipin, cholesterol and lecithin. The drug-loaded nano-vesicle can specifically target macrophages in a sepsis immunosuppression stage, has an intracellular response release function, recovers BMAL1 and IGF2BP2-ATP6V1B2 / ATP6V0c axis functions by inhibiting NR1D1 expression, reconstructs a macrophage phagocytosis function and lysosome-dependent bacterium removal capability, and can be used for preparing a drug-loaded nano-vesicle with a specific targeting function. The survival rate of sepsis immunosuppression model animals is obviously improved; and the bacterial load is reduced. Compared with a traditional electroporation method, the preparation method disclosed by the invention has the advantage that the encapsulation efficiency of siRNA is remarkably improved.

The invention provides a silicon dioxide essence microcapsule which is characterized in that essence is coated in a hydrophobic organic compound in a mononuclear or multinuclear form to form a core layer; and silicon dioxide is a shell layer. The outer layer of the microcapsule is a silicon dioxide shell, the secondary outer layer is a hydrophobic organic compound which is solid at room temperature, and essence is dispersed in the secondary outer layer in a single-core or multi-core form. The preparation method adopts a soap-free method and comprises the following steps: mixing and emulsifying essence, an organic compound which is in a solid state at room temperature and a polyalkoxy siloxane precursor, and then performing hydrolytic condensation on the precursor on a water phase interface to form a silicon dioxide shell so as to realize coating. The method is environment-friendly, and a surfactant does not need to be added. The obtained microcapsule has the characteristics of high encapsulation efficiency, excellent long-acting slow release performance, excellent thermal response controlled release capability and capability of desorbing and reloading essence after being used, and has wide application prospects in the fields of daily chemicals, textiles, foods and the like.

The invention belongs to the technical field of algal polysaccharide extraction, and discloses a modification extraction method of algal asparagus polysaccharide and a method for preparing a lutein coating by using the algal asparagus polysaccharide. The modified extraction method comprises the following steps: extracting gracilaria lemaneiformis powder with a weak base solution, enabling lactide and polysaccharide to be subjected to grafting reaction through a redox initiation system under the assistance of ultrasonic waves, and then separating out a first polysaccharide component with high emulsibility and a second polysaccharide component with high gel property through graded alcohol precipitation. The two obtained functional components are used for coating xanthophyll, specifically, the first polysaccharide component emulsifies a xanthophyll oil phase to form a pre-emulsion, the second polysaccharide component is compounded with gelatin, a composite coacervate is formed on an oil drop interface by adjusting the pH value, and finally drying and curing are conducted. According to the method, two modified polysaccharides with definite functions can be synchronously obtained, the prepared xanthophyll coating is high in encapsulation efficiency, good in storage and processing stability and excellent in water dispersibility, and the problems that xanthophyll is unstable and difficult to apply are effectively solved.

The invention provides a spirotetramat polyurethane / polyurea microcapsule suspending agent as well as a preparation method and application thereof, and belongs to the technical field of pesticide preparations. The preparation method comprises the following steps: mixing an oil-phase solution containing spirotetramat raw medicine and polyisocyanate with a water-phase solution containing an emulsifier and a dispersant, carrying out shear emulsification, adding a water-soluble wall material monomer water solution containing polyamine and / or polyol, and carrying out an interfacial polymerization reaction; after the polymerization is finished, sequentially carrying out curing treatment and cooling, then adding an auxiliary agent, and blending to obtain the spirotetramat polyurethane / polyurea microcapsule suspending agent. The spirotetramat microcapsule suspending agent is prepared through an interfacial polymerization method, spirotetramat is coated in a polyurethane or polyurea capsule wall, and the spirotetramat microcapsule suspending agent has the advantages of being high in encapsulation efficiency, good in physical and chemical stability, long in pesticide effect lasting period, safer to non-target organisms (such as bees) and the environment and the like. The preparation method is stable in process, easy for industrial production and excellent in product performance.

This invention discloses a compound preparation for treating vascular dementia and its preparation method. The active pharmaceutical ingredient consists of protopanaxadiol and gallic acid in a 1:1 mass ratio, and the dosage form is liposomes. The preparation method includes extraction, mixing, liposome preparation, and optional freeze-drying steps: protopanaxadiol and gallic acid are extracted and purified from ginseng rhizome and Terminalia chebula fruit, respectively. After mixing, they are combined with phospholipids and cholesterol to prepare a lipid membrane. The membrane is then homogenized by hydration, low-temperature ultrasound, and high-pressure microfluidic jet to obtain a homogeneous liposome suspension, which can be further freeze-dried into a freeze-dried powder. The 1:1 composition of protopanaxadiol and gallic acid targets multiple points on the pathological mechanism of vascular dementia, showing significant therapeutic effects and broad application prospects. This preparation uses a liposome dosage form to improve the bioavailability of the active ingredient, the preparation process parameters are controllable, the active ingredient has high purity, the liposomes have uniform particle size, high encapsulation efficiency, and good stability.

The invention discloses a brain-targeted nasal-spray amphotericin B phospholipid complex and a preparation method and application thereof, and belongs to the technical field of pharmaceutical preparations, the brain-targeted nasal-spray amphotericin B phospholipid complex is prepared by adopting a film dispersion method, and the preparation method comprises the following steps: dissolving 15-hydroxystearic acid polyethylene glycol ester, phospholipid, cholesterol, amphotericin B and optional other auxiliary components in a solvent, removing the solvent until a film is formed, and drying to obtain the brain-targeted nasal-spray amphotericin B phospholipid complex. And carrying out hydration dispersion to obtain the amphotericin B-loaded phospholipid complex. According to the amphotericin B sodium deoxycholate injection, the problems that the traditional amphotericin B sodium deoxycholate injection is high in renal toxicity, the intrathecal injection is poor in solubility, and low in bioavailability and patient compliance, large in side effect and the like due to the fact that the traditional amphotericin B sodium deoxycholate injection is difficult to penetrate through a blood brain After nasal administration, the drug is quickly delivered into the brain, so that the slow release of the drug is realized, the intracerebral bioavailability of the drug is improved, high brain targeting and low systemic toxic and side effects are realized, and good clinical application value and market prospect are achieved.

This invention discloses a liquid-phase stable high-content coenzyme Q. 10 Composite microcapsules, their preparation methods and applications, and liquid-phase stable high-content coenzyme Q. 10 The composite microcapsules include the following ingredients: Coenzyme Q 10 The active ingredients, emulsifiers, co-emulsifiers, fillers, and anti-caking agents; the coenzyme Q... 10 The components are: 10-60 parts by weight of the active ingredient, 5-15 parts by weight of the active ingredient, 2-10 parts by weight of the emulsifier, 1-5 parts by weight of the co-emulsifier, 10-50 parts by weight of the filler, and 0.1-1 parts by weight of the anti-caking agent. This invention provides a liquid-phase stable high-content coenzyme Q10. 10 The composite microcapsules have both high encapsulation efficiency and long-term stability in the liquid phase, and have significant anti-fatigue and physical strength enhancement effects.

According to the invention, the problems of complex preparation process, low embedding transmission efficiency, poor dissolution and dispersibility of xanthophyll, low absorption and utilization rate and the like of the xanthophyll embedding carrier are solved. The preparation method of the Maillard reaction modified corn peptide loaded xanthophyll nanoparticles comprises the following steps: 1, preparing a chitosan oligosaccharide solution and a corn peptide solution; 2, adjusting the pH value of the solution; 3, preparing glycosylated corn peptide through Maillard reaction; 4, carrying out load combination on the glycosylated corn peptide and the xanthophyll; and 5, preparing the xanthophyll nanoparticles. The method has the advantages that 1, the embedding rate and the solubility of the lutein are effectively improved; 2, the stability and the dispersity of the xanthophyll nanoparticles are improved; the absorption and utilization rate is good, the nutritive value is high, and large-scale production is easy. The preparation method of the xanthophyll nanoparticles is simple in process and good in xanthophyll controlled release effect and has a targeted absorption function.

This invention discloses a highly stable amphotericin B liposome and its preparation method, belonging to the field of pharmaceutical formulation technology. This invention utilizes a methanol-water-chloroform ternary mixed solvent system to achieve complete molecular-level dissolution of the raw materials, eliminating the conventional drying process and directly hydrating and homogenizing. It also provides optimal formulation ratios and process parameters. The preparation method includes steps such as oil phase preparation, aqueous phase preparation, homogenization, incubation, cooling, filtration, volumetric filling, and lyophilization. This invention creatively proposes optimal formulation composition and process conditions, resulting in a product with high encapsulation efficiency, uniform particle size, and strong stability. Furthermore, the preparation process is simple and easily industrialized, solving the problems of complex preparation processes and poor stability of existing injectable amphotericin B liposomes, and possesses good clinical application value.

The application discloses NAD liposomes, a preparation method and application thereof, and belongs to the technical field of biology.The NAD liposomes are prepared from NAD, agaric and black bean extract prepared by a special fermentation process, cholesterol, water-soluble chitosan, phosphatidylethanolamine and vitamin E through low-pressure homogenization and high-pressure homogenization.It is verified by experiments that the NAD liposomes have high encapsulation efficiency, drug loading and stability, and the effect of improving sleep is improved.

This invention discloses an oil composition with a balanced fatty acid ratio and lipid-lowering effects, and its applications. The oil composition comprises vegetable oil, probiotics, phospholipids from camellia seed cake, gelatin, and sodium alginate. It effectively reduces serum triglyceride, total cholesterol, and low-density lipoprotein cholesterol levels while increasing high-density lipoprotein cholesterol levels, exhibiting a good lipid-lowering effect. Furthermore, when the oil composition is formulated into microcapsules, it exhibits good stability, encapsulation efficiency, and release rate. The oil composition also helps maintain the activity of probiotics, allowing for better preservation and efficacy. Based on this, this invention also provides a candy containing the oil composition, enriching the variety of products with lipid-lowering effects and promoting the development of lipid-lowering related products.

The application provides an edible active coating for preventing dry consumption under an ultralow temperature condition and a preparation method, and belongs to the technical field of active packaging. The application combines double-layer coatings, inhibits free radical oxidation generated by freezing through an inner antioxidant, and effectively locks water and blocks external oxygen permeation through the synergistic effect of an outer high-barrier structure, thereby significantly improving the freezing preservation effect of food, and the raw materials are natural and safe, the preparation method is reasonable, and the application has good practical application value. The application solves the problems of poor effects of edible freezing protection coatings suitable for extremely cold environments in the prior art.

The present invention relates to a novel ionizable lipid compound represented by formula (I) or a salt thereof, and lipid nanoparticles comprising the compound or the salt thereof. The lipid nanoparticles comprising the novel ionizable lipid compound according to the present invention have excellent nucleic acid encapsulation efficiency and high nucleic acid cell delivery efficiency.

This invention relates to a method for preparing resveratrol nanoparticles, belonging to the field of traditional Chinese medicine technology. Addressing the problems of low oral bioavailability and lack of colonic targeting of resveratrol, this invention provides a nanoparticle formulation composed of resveratrol, zein, and xylan. The formulation is prepared by dissolving resveratrol and zein in an organic phase, adding them dropwise to an aqueous xylan phase, and then evaporating the organic solvent. This invention utilizes the hydrophobic effect of zein to improve drug encapsulation efficiency and leverages the colonic degradation characteristics and anti-inflammatory activity of xylan to achieve synergistic effects, enabling precise release of resveratrol at sites of intestinal inflammation.

The invention relates to the technical field of functional materials, in particular to a preparation method of a melatonin modified sleep-aiding polyester master batch, which comprises the following steps: mixing melatonin and pretreated cucurbit [8] uril for inclusion, and freeze-drying to obtain an inclusion compound; then dispersing in a wall material solution, homogenizing at a high speed, adding tannic acid and carrageenan for cross-linking and curing, and performing spray drying to obtain microcapsules; and finally, carrying out melt blending on the microcapsules and the low-melting-point copolyester chips in a double-screw extruder, and carrying out temperature-controlled extrusion pelletizing. According to the process, the cucurbit [8] uril is utilized to form the molecular capsule, so that the thermal stability and the encapsulation efficiency of the melatonin are remarkably improved; meanwhile, chitosan is adopted as a base material and is matched with a composite wall material formed by cross-linking and curing tannic acid and carrageenan, so that decomposition and loss of active components in the high-temperature melting process are effectively prevented.

This invention relates to the field of pancreatic cancer drug technology, and discloses a drug delivery nanomaterial encapsulating a cell membrane and its preparation method. The preparation method includes: dissolving PLGA, a sonosensitive agent IR780, and an immunoadjuvant R837 in a mixed organic solvent of dichloromethane and ethanol to form an oil phase; adding the oil phase to a PVA aqueous solution, ultrasonically emulsifying to form an emulsion, then adding an isopropanol aqueous solution, stirring and solidifying, and removing the organic solvent to obtain drug-loaded nanoparticles IR780 / R837@PLGA; finally, mixing the drug-loaded nanoparticles with the cell membrane of Panc02 pancreatic cancer cells in DEPC water and ultrasonically treating to obtain the drug delivery nanomaterial M-IR780 / R837@PLGA encapsulated by the cell membrane of Panc02 pancreatic cancer cells. This material can effectively target pancreatic tumors and prolong their in vivo circulation time, thereby enhancing the targeted therapy and immunotherapy effects of pancreatic cancer.

This invention relates to the field of microencapsulation technology for natural products, specifically to a water-soluble ginger oleoresin microcapsule, its preparation method, and its applications. The invention uses modified starch, plant polysaccharides, etc., as wall materials and ginger oleoresin as the core material. The ginger oleoresin microcapsule product is obtained through processes such as ginger oleoresin molecule coating, emulsification, high-pressure homogenization, constant-temperature standing, and drying. This product features high gingerol content, good water solubility, and low spiciness in the solution. It also exhibits good stability in acidic solutions, meeting the requirements of high stability, no precipitation, low spiciness, and good heat sensitivity in low pH environments. It is suitable for use in food, beverages, health foods, pharmaceuticals, cosmetics, and other fields.

The present application relates to the technical field of pharmaceutical preparation, in particular to a lotus leaf alkaloid liposome emulsion, a preparation method and application thereof, wherein sucrose / sodium chloride solid particles are combined with a wrapping material by a dipping film formation method to prepare empty liposomes, and a drug lotus leaf alkaloid is coated to prepare a lotus leaf alkaloid liposome emulsion.The preparation method provided by the present application is simple in process; raw materials are widely sourced and green and environmentally friendly, without affecting the biological activity of active ingredients; the preparation process is mild in conditions, without the need for special equipment, without changing the pH value of the solution, suitable for transdermal and oral administration; the amount of organic solvent is small, the encapsulation rate is high, and the preparation is easy to scale up.

This invention relates to the field of liposome preparation technology, specifically disclosing a liposome co-loaded with icariin and tanshinone B and its preparation method. The liposomes use soybean lecithin and cholesterol as membrane materials, simultaneously encapsulating icariin and tanshinone B, and are prepared using a thin-film hydration-ultrasound method. The liposomes prepared by this invention have an average particle size of 110.49 nm, and the icariin encapsulation efficiency can reach 85.469%. They exhibit relatively uniform particle size distribution, good system stability, and strong controllability of the preparation process. This method can improve the dispersibility and stability of icariin and can serve as a nano-formulation platform for the co-delivery of icariin and tanshinone B.

The invention provides a liposome adjuvant as well as a preparation method and application thereof, and belongs to the technical field of biomedical engineering. A liposome adjuvant system comprises an aqueous phase and a lipid component, the aqueous phase comprising a water-soluble adjuvant present in the internal aqueous phase of the liposome and an aqueous phase medium, the aqueous phase medium comprising from 1% w / v to 15% w / v of a sugar and / or a sugar alcohol. The liposome adjuvant system provided by the invention has higher encapsulation efficiency, stability and immunocompetence, and meanwhile, the influence of common buffer solution, sodium chloride and other salt components on the function of the liposome adjuvant is avoided. In addition, according to the liposome adjuvant system, the cell toxicity and the in-vivo toxicity of the liposome adjuvant system can be reduced by reducing the dosage of cationic lipids such as DOTAP and DOTMA.

The present application belongs to the field of gene drug delivery, and particularly relates to a kind of lipid compound with glutaric acid skeleton and lipid carrier, nucleic acid lipid nanoparticle composition and pharmaceutical preparation based thereon.The compound with the structure of formula (I) of the present application can be prepared into lipid carrier alone or together with other lipid compounds.The lipid carrier has high encapsulation efficiency for nucleic acid drugs, which helps to improve the delivery efficiency of nucleic acid drugs in vivo;Multiple biodegradable functional groups are contained, so that the lipid metabolism is fast, and the biological safety is high.Moreover, the lipid carrier can also deliver nucleic acid drugs to organs that need to be enriched, and has good application prospect.

The invention relates to the technical field of biological medicine manufacturing, and provides a water-soluble microcapsule and a preparation method thereof.The preparation method comprises the following steps that an emulsifying agent, a thickening agent and a water-soluble monomer are dissolved in water, and an emulsion continuous phase is obtained; the content of the emulsifier in the water is 0.5%-5%; the content of the thickening agent in the water is 0.5%-5%; the ratio of the water-soluble monomer to the water is (1: 100)-(20: 100); the water-soluble monomer is introduced to increase the viscosity of a water phase and participate in construction of a shell layer, so that diffusion of a core material to the water phase is effectively inhibited, and high encapsulation efficiency and high drug loading capacity are realized; the process parameters such as the emulsifying rotating speed and the monomer proportion are easy to adjust, microcapsules with the particle size ranging from 1 micrometer to 800 micrometers can be prepared in a controlled mode, the packaging requirements of different water-soluble active substances such as vitamins and enzyme preparations are met, and the product adaptability is good.

The present application relates to the technical fields of biological medicine and gene therapy, and particularly relates to a protonatable cationic lipid, a nucleic acid delivery system and application thereof. The protonatable cationic lipid has a structure as shown in formula (I), and contains a protonatable group such as a hydroxyl group or an amino group at the end. The protonatable cationic lipid can form a lipid nanoparticle for loading various nucleic acid substances. The lipid nanoparticle nucleic acid delivery system has high cell transfection efficiency in vitro, and can be targeted to organs such as liver in vivo to achieve efficient gene editing. The lipid nanoparticle constructed by the protonatable cationic lipid provided by the present application has high encapsulation efficiency, good stability and excellent delivery efficiency, and has a wide application prospect in the fields of gene therapy and cell engineering.

The invention discloses a vaccine composition which comprises an antigen and at least one drug carrier, the drug carrier is a polymer microsphere with a cavity structure, the polymer microsphere is subjected to freeze thawing treatment, so that openings or pores are formed in the surface of the polymer microsphere, the preparation method of the vaccine composition comprises the following steps: mixing the drug carrier with a solution containing an antigen, preferably loading the antigen on the drug carrier in modes of infiltration and / or adsorption and the like, and then centrifuging to remove supernate to obtain polymer microspheres loaded with the antigen, preferably, the polymer microspheres loaded with the antigen are loaded with the solution containing the antigen. The antigen is an influenza antigen, such as an influenza hemagglutinin protein, or the antigen is a herpes zoster virus antigen, such as a herpes zoster virus antigen gE glycoprotein.

This invention provides a liposome composition with soothing effects and its preparation method, relating to the field of cosmetic technology. The liposome composition comprises the following raw materials: active ingredients and solvents; the active ingredients include dioleoylphosphatidylglycerol, cholesterol hemisuccinate, N-palmitoylhydroxyproline cetyl ester, phosphatidylinositol, phytosphingosine, frankincense resin extract, and brown algae extract. This invention, through a specific component ratio of the liposome raw materials and a specific combination of plant extracts, significantly improves the encapsulation rate of plant extracts and the stability of the composition, thereby significantly enhancing the soothing and anti-inflammatory effects, providing better product choices for users with sensitive skin.

The invention belongs to the technical field of medicine, and particularly relates to a degradable sustained-release local anesthetic microsphere and a preparation method and application thereof.The degradable sustained-release local anesthetic microsphere comprises a drug core and a carrier shell wrapping the outer layer of the drug core, the drug core is a local anesthetic, and the carrier shell wraps the outer layer of the drug core. The carrier shell is a composite material of a modified polylactic acid-glycolic acid copolymer (PLGA) and a biocompatible auxiliary material. According to the invention, the modified PLGA is used as a carrier material, the hydrophilicity and the drug compatibility of the carrier are improved through PEG modification, the encapsulation efficiency (greater than or equal to 85%) and the drug loading capacity (10%-30%) of the local anesthetic are obviously improved, the drug waste is reduced, and the drug utilization rate is improved.

The invention discloses a preparation method of pH gradient active drug loading type maqui berry anthocyanin-curcumin compound lipidosome, belongs to the fields of biological medicines, food science and nutritional health-care products, and particularly relates to a nano drug delivery system and preparation of functional foods and dietary supplements. According to the method, the water-soluble maqui berry anthocyanin and the fat-soluble curcumin are efficiently entrapped at the same time by utilizing a unique double-layer structure of the lipidosome and through a film dispersion-hydration technology. Soybean lecithin and cholesterol are used as main membrane materials, tea polyphenol or vitamin E and other antioxidants are combined, and curcumin is induced to transmembrane-transfer from a lipid bilayer membrane to an internal water phase through precise pH gradient control and mild heating, so that the encapsulation efficiency of curcumin is remarkably improved. Then, through the steps of high-pressure microjet homogenization, low-temperature dialysis and the like, the maqui berry anthocyanin-curcumin compound liposome which is uniform in particle size, high in stability and excellent in encapsulation efficiency is prepared.

The invention discloses sinomenine hydrochloride solid lipid nanoparticles, gel, a preparation method and application, by exploring the preparation method of the sinomenine hydrochloride solid lipid nanoparticles HA-SH-SLNs, when the mass ratio of sinomenine hydrochloride to soya bean lecithin to hyaluronic acid modified targeting molecule DSPE-PEG-HA to poloxamer Pluronic F-68 is (0.3-0.7): (1-4): (0.0125-0.1): (1-4), the gel can be used for preparing the sinomenine hydrochloride solid lipid nanoparticles HA-SH-SLNs. The sinomenine hydrochloride solid lipid nanoparticles which are uniform in particle size, high in encapsulation efficiency, high in drug loading capacity and stable in quality are obtained, and the preparation method of the sinomenine hydrochloride solid lipid nanoparticle gel is further researched. The obtained sinomenine hydrochloride solid lipid nanoparticle gel HA-SH-SLNs-Gel is good in stability and small in hemolysis rate, the in-vivo residence time is longer than that of sinomenine hydrochloride solid lipid nanoparticles, the drug release period is delayed, and better articular cavity injection administration is facilitated.

This invention relates to an oral microemulsion containing elemene, its preparation method, and its uses. The microemulsion has a simple formulation, high drug loading capacity, good stability, and a simple preparation process, making it suitable for the prevention and treatment of gastric diseases, especially gastric cancer.

This invention relates to a tofenamic acid liposome, its preparation method, and its application. The raw materials for preparing the tofenamic acid liposome include phospholipids, cholesterol, and tofenamic acid. The tofenamic acid is encapsulated in a lipid bilayer. The tofenamic acid liposome of this invention improves the solubility of tofenamic acid and has a high encapsulation efficiency and drug loading capacity.