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High molecule liposome and uses thereof

A liposome and macromolecule technology, applied in the field of preparation and application of liposomes, can solve problems such as biocompatibility and biodegradability limitations, and achieve simple and easy preparation process, stable system, and different conditions. high effect

Inactive Publication Date: 2008-08-13
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, scholars from various countries also try to replace synthetic phospholipids with cheap synthetic polymer materials to prepare lipid vesicles to reduce costs and increase practicability, but in vitro tests have proved that such polymer lipid vesicles are biocompatible. limitations of sex and biodegradability

Method used

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  • High molecule liposome and uses thereof
  • High molecule liposome and uses thereof
  • High molecule liposome and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: This example discusses the effect of different ratios of carboxymethyl chitosan octadecyl quaternary ammonium salt (QACMC) and cholesterol on the particle size of polymer liposomes when the reverse evaporation method is adopted.

[0039] Carboxymethyl chitosan octadecyl quaternary ammonium salt (weight-average molecular weight is 20,000, quaternary ammonium salt substitution degree is 20.0%) and cholesterol in different proportions are co-dissolved in dichloromethane, and mixed to obtain solution I ; Prepare deionized aqueous solution II, wherein the ratio of solution I and solution II is 1: 2; after mixing the two solutions, fully ultrasonic emulsification, dichloromethane is distilled under reduced pressure on a rotary evaporator to obtain polymer liposomes solution. The polymer liposome is positively charged cationic polymer liposome, and its surface Zeta potential can reach +52.72mv. As can be seen from Table 1, adjusting the mass ratio of QACMC and chol...

Embodiment 2

[0043] Example 2: This example is a discussion on the preparation of polymer liposomes using carboxymethyl chitosan long-chain alkyl quaternary ammonium salts with different molecular weights, using the reverse evaporation method.

[0044] Weigh 20 mg of carboxymethyl chitosan dodecyl quaternary ammonium salt (the weight-average molecular weight is 10,000, and the degree of substitution of quaternary ammonium salt is 80.0%), 10 mg of soybean sterol, dissolve in 3 ml of methylene chloride, and shake to obtain a uniform solution I; Weigh 3.0mg bovine serum albumin (BSA) and dissolve it in 5ml PBS (pH=7.4) buffer solution, shake well to fully dissolve BSA to obtain aqueous solution II; then blend and emulsify the above two solutions I and II, and ultrasonically After 10 minutes, after forming a stable emulsion, put it into an eggplant-shaped bottle, distill it under reduced pressure on a rotary evaporator until the methylene chloride volatilizes completely, and separate the unwrap...

Embodiment 3

[0046] Embodiment 3: This embodiment is an example of using other types of chitosan long-chain alkyl quaternary ammonium salts to prepare polymer liposomes except carboxymethyl chitosan octadecyl quaternary ammonium salt.

[0047] Take by weighing carboxymethyl chitosan octyl quaternary ammonium salt (weight-average molecular weight is 2000, degree of substitution of quaternary ammonium salt is 40.0%) 15mg, glucoside 12mg, be dissolved in 3ml methylene chloride, shake evenly to obtain solution I, Then put it into an eggplant-shaped bottle, distill under reduced pressure on a rotary evaporator, and feed nitrogen from time to time until the dichloromethane is completely volatilized, and then vacuum-dry at room temperature for 24 hours; weigh 3.0 mg vincristine and dissolve it in 5 ml PBS (pH =7.4) In the buffer solution, shake well to make the vincristine fully dissolve to obtain the aqueous solution II; then add the above-mentioned 5.0ml vincristine solution II in the eggplant-s...

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Abstract

The invention relates to a preparation and application of a new macromolecule lipidosome. The structure is a vesicle which has a lipide dual-layer structure containing duoparental chitosan longchain alkyl quaternary ammonium salt as well as phospholipid with micromolecule. The chitosan longchain alkyl quaternary ammonium salt is characterized in that: the weight average molar mass is bigger than 2000; the chitosan longchain alkyl quaternary ammonium salt can be dissolved in chloroform and other organic solvent, which contains carboxyl, amino or quaternary ammonium salt radical and concretely is one of the duoparental chitosan longchain alkyl quaternary ammonium salt disclosed by 200710056993.4 of China Invention Patent. The surface of the macromolecule lipidosome can be connected with various Liver-Targeted preparations. The macromolecule lipidosome can carry water-soluble, oil-dissolving or duoparental materials including medicine, protein, gene, nutrition, vitamin, magnetic granule and quantum dot, etc. The macromolecule lipidosome has the advantages of high wrapping rate, simple and convenient operation, strong applicability and low cost; the macromolecule lipidosome after being covered has even distributed granule diameter which can be lower than 20nm for the smallest, has stable system and strong functions for carrying medical tiny balls and slowly controlling as well as releasing.

Description

technical field [0001] The invention relates to the preparation and application of a new polymer liposome. The invention belongs to the preparation and application technology of liposome. Background technique [0002] Liposome is a molecular orderly assembly formed spontaneously by phospholipids in water by hydrophobic association. It is the water phase, and the oil phase is between the bimolecular membranes. Liposomes are widely used, the preparation process is simple, and the methods are diverse. Its particle size is in the nanoscale mesoscopic range, and has many unique physical and chemical properties. The particle size can also be controlled according to needs; it can be used for the preparation of nanoparticles, Clinical testing and diagnosis, control of catalytic reactions, simulated membrane research, delivery of genes or other substances into cells in vivo and in vitro, etc. Liposomes have been used as drug carriers since the 1970s. Due to their simple preparatio...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K47/36
Inventor 常津梁晓飞
Owner TIANJIN UNIV
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