The invention discloses a concentrated multienzyme cleaning solution and a preparation method thereof, wherein the concentrated multienzyme cleaning solution is prepared from the following raw materials in parts by weight: 1 to 5 parts of organic preservative, 200 to 400 parts of surfactant, 20 to 65 parts of enzyme stabilizer, 40 to 80 parts of propylene glycol, 30 to 80 parts of triethanolamine, 10 to 60 parts of sodium sulfite, 0.1 to 5 parts of calcium chloride, 20 to 60 parts of sodium citrate, 10 to 65 parts of citric acid, 0.1 to 1 part of antifoaming agent, 30 to 80 parts of bio-enzyme, and appropriate deionized water; and the method for preparing the concentrated multienzyme cleaning solution comprises the following steps: dissolving the preservative into the propylene glycol, then adding the surfactant, the triethanolamine and the antifoaming agent into the obtained mixture so as to obtain a solution A; heating the deionized water, and dissolving the enzyme stabilizer, the sodium sulfite, the calcium chloride, the sodium citrate and the citric acid into the deionized water, then cooling the obtained mixture so as to obtain a solution bio-enzyme B; and finally, mixing the solution A and the solution B so as to obtain the finished product of the concentrated multienzyme cleaning solution. The concentrated multienzyme cleaning solution and the preparation method thereof have the advantages: the enzyme stabilizer is adopted to effectively protect the activity of the bio-enzyme, and used with the surfactant and the preservative, so that the obtained finished product is stable in performance, long in storage time and strong in capacity of decomposing biological tissues.