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627results about How to "Improve microenvironment" patented technology

Cementitious material reinforced with chemically treated cellulose fiber

The present invention relates to a fiber-reinforced cement based or cementitious material, and process for making, where the reinforcing fiber is a chemically treated cellulose or non-cellulose fiber. The fiber reinforced cementitious material includes (i) cement, (ii) optionally, sand, aggregate, or sand and aggregate, and (iii) chemically treated fibers having a polyvalent cation content of from about 0.1 weight percent to about 5.0 weight percent based on the dry weight of the treated fibers. The present invention further provides for a fiber reinforced cementitious material having a weak acid content of from about 0.5 weight percent to about 10 weight percent based on the dry weight of the treated fibers.
Owner:GP CELLULOSE GMBH

Porket premix compound and porket creep feed prepared by the premix compound

The present invention provides a porket premix compound and a porket creep feed prepared by the porket premix compound. The porket premix compound is composed of the components of a yeast powder, an energy-nutrient accelerating agent, a flavoring agent, minor elements, etc. The porket creep feed is composed of the components of swelled corn flour, swelled soybean flour, swelled wheat flour, low-protein whey powder, porket premix compound, etc. The porket premix compound of the invention fully takes the electrolyte balance into consideration; supplies various minor elements and vitamins required by the porket, promotes the growth and is used for preparing the excellent proket creep feed. The general dosage is 1% of the weight of the porket creep feed. At least 85% of the porket creep feed passes through a 60 mesh sieve so that the granularity of the feed stuff is smaller and the absorption of the porket to the nutrition substance is facilitated. The porket creep feed further has the advantages of sustaining the integrality of the mucous membrane, overcoming the weaning stress, increasing the feed intake, increasing the digestion and absorption rate, reducing the feed-meat ratio, realizing the effect of continuous body weight growing after the weaning.
Owner:河南雄峰科技股份有限公司

High-strength collagen base artificial bone repair material

The invention provides a high-strength collagen base artificial bone repair material which can be used for repairing the bone defect of bearing parts of a human body. The high-strength collagen base artificial bone repair material has chemical composition and structure formed by self-assembly of nanometer calcium phosphate and collagen molecules, so that the high-strength collagen base artificial bone repair material has a biomimetic mineralization structure similar to the natural bone tissue of the human body. In terms of mechanical properties, the material has the mechanical strength similar to that of the cortical bone of the human body and can be used for repairing the bone defect of the bearing parts of the human body. The invention also provides a preparation method of the high-strength collagen base artificial bone repair material.
Owner:BEIJING ALLGENS MEDICAL SCI & TECH

Human umbilical cord mesenchymal stem cell (HUMSC) anti-hepatic fibrosis injection and preparation method thereof

The invention discloses a human umbilical cord mesenchymal stem cell (HUMSC) anti-hepatic fibrosis injection and a preparation method thereof. The injection is composed of 10%-50% of human albumin stock solution, 49.5%-89.5% of acetated Ringer's solution and 0.5% of heparin calcium, wherein, the concentration of the human albumin stock solution is 10%, and 1ml of the injection contains 6*10<5>-7*10<5> HUMSCs. The preparation method comprises the following steps: providing an MSC preserving fluid and pre-cooling the MSC preserving fluid for later use, wherein, the MSC preserving fluid comprises the human albumin stock solution, the Acetated Ringer's solution and the heparin calcium; providing the HUMSCs and adding the HUMSCs to the MSC preserving fluid; and adjusting the number of the HUMSCs added to the MSC preserving fluid to be 6*10<5>-7*10<5> HUMSCs / ml by means of re-suspending. The injection provided by the invention has the advantages of obvious curative effect and high stability, and is convenient in storage and transport and safe in use, thus being capable of bringing gospel to patients with hepatic fibrosis.
Owner:中源协和生物细胞存储服务(天津)有限公司

Cell preparation for treating osteoarthritis and preparation method thereof

The invention provides a cell preparation for treating osteoarthritis and a preparation method thereof. The cell preparation mainly comprises mesenchymal stem cells, differentiation-induced stem cells, platelet-rich plasma and dispersion media thereof. Compared with the prior art, the cell preparation for treating osteoarthritis has the advantages that the mesenchymal stem cells are mixed with the differentiation-induced stem cells and compounded with the platelet-rich plasma and can be dispersed in the joint cavity, so that the immune regulation function of the mesenchymal stem cells can be exerted, the microenvironment of the whole joint cavity can be improved, and inflammation remission and injury repair are facilitated; tissues can be repaired after the differentiated mesenchymal stem cells are implanted into the body, so that the repair process is shortened.
Owner:广东佰鸿干细胞再生医学有限公司

Micro-nano composite dual-layer skin framework and manufacturing method thereof

The invention discloses a micro-nano composite dual-layer skin framework and a manufacturing method thereof which are applied to the skin regenerative tissue engineering. The manufacturing method includes the steps of firstly, preparing a nanofiber membrane layer by the electrospinning technique and utilizing the same as a surface layer of skin; secondly, preparing a three-dimensional framework by direct writing on the nanofiber membrane layer by an electrohydrodynamic inkjet printing method and utilizing the same as dermis. Specifically, the nanofiber membrane refers to a layer of nanofibers uniform in diameter; the nanofibers provide suitable surface morphology for cell adhesion and growth, thereby facilitating cell adhesion and growth; the three-dimensional framework refers to a three-dimension space composed of nanofibers by the electrohydrodynamic inkjet printing method, provides a microenvironment for growth of cells, and facilitates cell infiltration and proliferation; both the nanofiber membrane and the three-dimensional framework are made from biodegradable high-polymer material. Compared with the conventional artificial skin framework, the micro-nano composite dual-layer skin framework has the advantages of high plasticity, good mechanical property, three-dimension structure with high porosity, and high biocompatibility.
Owner:SHANGHAI UNIV

Preparation method for conduction and sustained release type nervous tissue engineering scaffold

The invention relates to a preparation method for a conduction and sustained release type nervous tissue engineering scaffold. The method comprises the following steps: silk fibroin Silk and lactic acid-caprolactone copolymer are dissolved in a solvent, dissolved and stirred to obtain a solution, then polyaniline and camphorsulfonic acid are added and stirred and mixed uniformly to obtain a cortex electrospinning solution, and NGF (nerve growth factors) are dissolved in ultrapure water completely to obtain a core electrospinning solution; and the cortex electrospinning solution and the core electrospinning solution are accommodated in an injector respectively for coaxial electrospinning, and then fumigation treatment and vacuum drying are performed to obtain the conduction type nervous tissue engineering scaffold. According to the prepared nanofiber scaffold, the nerve repair speed is increased from ways including external electrical stimulation (conductive polymer), biochemistry (NGFs), a topological structure required by nerve regeneration (orientation guide) and the like. The method is simple to operate, good in repeatability and high in economic benefit, and provides novel experimental thought for nerve defect repair in clinical application.
Owner:诺一迈尔(山东)医学科技有限公司

Microorganism composite fungus agent for promoting agricultural waste quickly rotten and its making method

A composite bacteria preparation for quickly putrefying agricultural waste is composed of living bacteria (bacillus, actinomycess, yeast and fungus) and adsorptive carrier (zeolite, bran, bone powder, fish powder and brown sugar). Its advantages are high putrefying efficiency, low cost, less loss of nutrients and improving soil.
Owner:李鸣雷

Soil conditioner, preparation method and application thereof

The invention provides a soil conditioner which comprises the following components by weight percentage based on the dry basis, 70-90% of ammonibacteria sludge, 0.1-10% of active ingredients and 5-25% of accessory. The invention also provides a preparation method for the soil conditioner and an application. By ammoniating the soil, the invention increases the nutrient content of the soil conditioner and simultaneously turns the sludge and organic material which pollute or harm the environment into a resource, thus not only solving the handling problem of waste, improving the environment, recycling the waste and opening up a new approach to the raw material required by the production of the soil conditioner.
Owner:BEIJING HAIYA DIDA BIOCHEM TECH

Feed micro-ecological preparation, anti-nutritional substance-free feed and preparation method thereof

The present invention discloses a feed micro-ecological preparation, an anti-nutritional substance-free feed and a preparation method thereof, and belongs to the technical field of feed. The feed micro-ecological preparation is prepared by fermenting the following raw materials in parts by weight: 1.0-1.2 parts of Saccharomyces cerevisiae, 0.2-0.6 part of Enterococcus faecalis, 1.5-2.0 parts of Bacillus subtilis, 0.2-0.6 part of Bacillus coagulans, 0.1-0.15 part of Bacillus licheniformis, 0.1-0.3 part of Bacillus lentus, 0.2-0.5 part of Clostridium butyricum and 993.05-995.15 parts of liquid medium. After the feed micro-ecological preparation is mixed with feed raw materials, enzymes produced in the process of microbial strain fermentation can decompose anti-nutritional substances in feed and increase the feed utilization rate; at the same time, microbial strains also have effects in improving animal intestinal environment, maintaining intestinal flora balance, inhibiting the growth of pathogenic bacteria, and improving animal immunity.
Owner:SUIPING GUANGYUAN FEED TECH CO LTD

Conditioner for quick desalination of coastal saline soil

The invention relates to a conditioner for the quick desalination of coastal saline soil, which comprises organic fertilizer, gypsum powder and polyacrylamide, wherein the application amount of the organic fertilizer is 5 to 10 cubic meters / mu, the application amount of the gypsum powder is 5 to 10 cubic meters / mu, and the application amount of the polyacrylamide is 1 to 1.3 kilograms / mu. Aiming at the special demands of sowing and seedling keeping in a coastal saline and alkaline area, the conditioner for the quick desalination of the coastal saline soil is developed, has the advantages of simple operation during use, local production of raw materials, low cost and quick desalination, can also be used for regulating the moisture and nutrients of the soil, maintaining water and soil nutrients, improving the soil and microenvironment of seeds and improving crop yield, and is a novel nontoxic conditioner for soil liquid manure.
Owner:INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI

Rhizoma paridis planting method

The invention discloses a rhizoma paridis planting method which comprises the following steps: (1) selecting and treating seeds; (2) carrying out bud forcing treatment on the seeds; (3) manufacturing a seedling bag; (4) sowing the seeds; (5) arranging a planting site; (6) carrying out transplantation; and (7) carrying out field management. Rhizoma paridis seeds cultivated by the invention are high in germination rate and survival rate of transplanting; and through adoption of the rhizoma paridis planting method, use of a chemical fertilizer and a pesticide can be reduced, the soil is optimized, the yield of rhizoma paridis can be increased, and the quality of the rhizoma paridis can be improved.
Owner:迪庆经济开发区箐园生物科技开发有限公司

Production of biological diesel oil by fixed enzyme method

A process for producing biological diesel oil by fixed enzyme method is carried out by taking sodium alginate, kaoline, glutaraldehyde, gelatin and lactose as co-fixer, fixing the lipase, piling the spherical fixed enzyme in filled bed columnar reactor naturally, alcoholyzing animal and plant oil fatty with biological diesel oil as solvent and producing biological diesel oil. It is cheap, has less consumption and by product and better quality. It can be used for continuous production.
Owner:茂名市泓宇能源科技有限公司

Electrochemical sensor used for instantly monitoring and detecting water biotoxicity, and apparatus thereof

The invention discloses an electrochemical sensor used for instantly monitoring and detecting the water biotoxicity. The electrochemical sensor comprises a work electrode, a counter electrode, a reference electrode and a electrolytic cell; the work electrode is a polymer fixed microbial electrode, and the polymer fixed microbial electrode comprises an electrode and a polymer and bacteria mixed layer coated outside the electrode; and the material of the above polymer is gelatin or chitosan, the above bacteria comprise Escherichia coli and / or yeast, and the electrode is a glassy carbon electrode, a gold electrode, a platinum electrode or a boron-doped diamond film electrode. The invention also discloses an apparatus of the electrochemical sensor used for instantly monitoring and detecting the water biotoxicity. The electrochemical sensor can instantly monitor the water biotoxicity change and detect the water biotoxicity value, can realize instant, online and continuous detection, and has the characteristics of high analysis sensitivity, low cost, simple operation, portability and the like.
Owner:TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI

Flour having low glycemic index and making method thereof

The invention discloses flour having low glycemic index and a making method thereof. The flour is made from, by weight, wheat flour 35%-45%, soybean flour 35%-45%, buckwheat powder 1%-1.5%, oat powder1%-1.5%, isolated soy protein powder 4%-4.5%, dietary fibers 4%-4.5%, vitamins 0.5%-1.5% and mineral substance 0.5%-1.5%. The flour is based on the low-GI-value (a GI value is below 55) coarse cereal powder, uses the added protein, dietary fibers, vitamins, mineral substance and the like as nutritional materials, has good eating taste and is a high-fiber-content and low-fat healthy food. The flour can delay postprandial blood sugar rise to some degree and is suitable for high-glucose people, patients with diabetes and populations needing low-glycemic-index foods.
Owner:ANHUI TONGFU FOOD

Complex nutrient additive block special for yaks

The invention discloses a block for the nutrient balance and health of special livestock yaks under the low-oxygen condition of the Tibet plateau, which consists of the components of molasses, urea, salt, corn flour, calcium hydrogen phosphate, bentonite, oligosaccharide, zinc sulfate, ferrous sulphate, potassium iodide, sodium selenite and cobalt chloride. The block provides mineral elements andcrude proteins of which the yaks are lack due to unbalanced ingestion, improves the constitution of the yaks, enhances disease resistance, reduces the using amount of antibiotics, and also can increase the hemoglobin content of the blood of the yaks to further improve the adaptability of the yaks to a low-oxygen environment.
Owner:LANZHOU UNIVERSITY

Combined fertilizer synergist containing polyglutamic acid, phosphate solubilizing bacteria and potassium bacteria and preparation method and application of combined fertilizer synergist

ActiveCN104276882AStrong ability to decompose inorganic phosphorusImprove bioavailabilityFertilizer mixturesPhosphate ionPhosphate solubilizing bacteria
The invention discloses a combined fertilizer synergist containing polyglutamic acid, phosphate solubilizing bacteria and potassium bacteria. The combined fertilizer synergist comprises the following components in proportion: phosphate solubilizing bacteria, potassium bacteria and polyglutamic acid at the mass ratio of (0.1-1) to (0.1-1) to (0.001-0.1). The invention also discloses a preparation method and application of the fertilizer synergist. The phosphate solubilizing bacteria disclosed by the invention are capable of effectively releasing phosphate anions (PO4<3+>) in soil or fertilizer; the potassium bacteria are capable of effectively releasing potassium ions (K<+>) in the soil or fertilizer; after being compounded with the phosphate solubilizing bacteria and the potassium bacteria, the polyglutamic acid not only can provide a good growth environment for survival of the phosphate solubilizing bacteria and the potassium bacteria, but also can fully chelate nutrient elements released by the phosphate solubilizing bacteria and the potassium bacteria; and due to the synergistic effect, the effect of solubilizing phosphate and potassium is relatively outstanding, therefore the combined fertilizer synergist can be cooperatively used together with fertilizers, the utilization rate of the fertilizer is increased, and the combined fertilizer synergist can also be directly fertilized as a bacterial manure.
Owner:轩凯生物科技(滁州)有限公司

Low-expression CYP7A1 hepatic cell and constructing method thereof

The invention discloses a low-expression CYP7A1 hepatic cell and a constructing method thereof. A method for inhibiting the CYP7A1 expression level of the hepatic cell comprises the step of introducing an encoding gene of small-interference RNA which is used for inhibiting the CYP7A1 gene expression into the hepatic cell so as to inhibit the CYP7A1 expression level of the hepatic cell. A slow virus vector system can be used for introducing the encoding gene of small-interference RNA which is used for inhibiting the CYP7A1 gene expression into the hepatic cell. An experiment proves that the method of the invention can obviously regulate the expression level of the CYP7A1 gene of the hepatic cell in an mRNA level and a protein level downwards and can further effectively reduce the secretoryvolume of total bile acid. The Low-expression CYP7A1 hepatic cell and the constructing method thereof can be used for the fundamental research and the clinical application of biologic artificial liver supporting treatment and have a wide application prospect.
Owner:FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY

Thermosensitive dual-administration nanocomposite hydrogel as well as preparation method and application thereof

The invention belongs to the technical fields of biomedical materials and medical apparatus, and in particular relates to a thermosensitive dual-administration nanocomposite hydrogel as well as a preparation method and application thereof. The preparation method of the thermosensitive dual-administration nanocomposite hydrogel comprises the following steps: preparing a poly (N-isopropylacrylamide) nano microsphere and loading a growth factor, preparing a poly (N-isopropylacrylamide-co-acrylic acid) nano microsphere and loading an anti-inflammatory drug, and preparing the thermosensitive dual-administration nanocomposite hydrogel. The finished product takes sodium alginate as a matrix phase and uses two poly (N-isopropylacrylamide) based thermosensitive nano gel microspheres which have different LCSTs and are respectively loaded with the anti-inflammatory drug and the growth factor as a dispersion phase; and by controlling the temperature of the composite hydrogel to be higher than or lower than LCST of the nano microspheres, the release of the anti-inflammatory drug and the release of the growth factor are controlled, so that controlled release of the anti-inflammatory drug and the growth factor in inflammatory stage and proliferative stage of a deep wound repairing process is achieved.
Owner:JINAN UNIVERSITY

Fixing device for orthopedic surgery recovery treatment and using method thereof

The invention discloses a fixing device for orthopedic surgery recovery treatment. The fixing device comprises a first splint, a second splint and auxiliary treatment components; one side of the first splint and one side of the second splint are rotatably connected with each other through a pin shaft, upper rope rings and lower rope rings are fixedly connected to the top ends and the lower ends of the first splint and the second splint respectively, the auxiliary treatment components are fixedly connected to the middle of the first splint and the middle of the second splint respectively, and medicine liquid adding holes are formed in the surfaces of the auxiliary treatment components. The fixing device for orthopedic surgery recovery treatment is simple in using method and structure, convenient to assembly, disassembly and use, capable of adapting to different groups and different using parts, greatly improving the practicability and applicability and providing a good microenvironment for the surgery part and beneficial for promoting blood circulation and improving the treatment recovery effect. The invention further discloses a using method of the fixing device for orthopedic surgery recovery treatment.
Owner:王爱敏

Preparation method of aptamer photoelectrochemical sensor for high-sensitivity high-selectivity detection of MC-LR

The invention relates to a preparation method of an aptamer photoelectrochemical sensor for high-sensitivity high-selectivity detection of MC-LR. The preparation method comprises preparing titanium dioxide nanotubes on a titanium matrix by an anodization method, carrying out modification with cadmium sulfide nanoparticles, functionally assembling chitosan and glutaraldehyde to the surface of an electrode so that a photoelectric biological interface is built, and bonding a MC-LR aptamer with a specific base sequence to the surface of the base electrode by chemical bonding so that the MC-LR aptamer photoelectrochemical sensor is prepared. Through combination of a supersensitive photoelectric analysis technology and an aptamer technology with a MC-LR specific recognition function, the preparation method realizes high-sensitivity high-selectivity analysis detection of MC-LR, and has the MC-LR detection limit of 5.3*10<-12> mol / L and a linear detection range of 7.2*10<-8> to 8*10<-12> mol / L. Compared with the prior art, the preparation method has the advantages of simple processes, fast response and low cost and can realize high-sensitivity high-selectivity analysis of MC-LR.
Owner:TONGJI UNIV

Protein A immuo adsorption material and preparing method

The present invention relates to a protein A immunoadsorption material for purifying blood and its preparation method. Said protein A immunoadsorption material is a high-molecular material which is obtained by using agarose gel as carrier matrix, making said agarose gel be reacted with diglycidyl ether coupling reagent to obtain active carrier, then making said active carrier and protein A implement coupling reaction. Said invention can be used for making clinical immunoadsorption therapy.
Owner:GUANGZHOU KONCEN BIOSCI +1

Microbial inoculum and method for preparing biological organic fertilizer by using same

The invention relates to microbial inoculum which contains bacillus subtilis CIP3 CGMCC NO.3171, bacillus sp. CIP1 CGMCC NO.3172 and Pichia sp. CIP2 CGMCC NO. 3170. The invention also relates to a method for preparing biological organic fertilizer by using the microbial inoculum. Three bacteria which are all beneficial bacteria are mutually matched and have extremely strong decomposition capacityto organic matters, and the obtained decomposed products have no extraneous odors; and the three bacteria can improve the soil microenvironment, accelerate the growth of crops and reduce plant diseases and insect pests while being used in soil. The method for preparing the biological organic fertilizer has the advantages that the adopted raw materials are all agricultural and industrial leftovers,and the cost is low; the roles of three microbes can be fully exerted, various organic matters can be ensured to be decomposed, and the organic matter contents of soil are increased.
Owner:迟仁立 +1

Repairing gel containing growth factors and preparation method of repairing gel

The invention belongs to the medicine field, and particularly relates to repairing gel containing growth factors and a preparation method of the repairing gel .The repairing gel is prepared from 0.001%-0.02% of the growth factors, 0.05%-1% of alkaline polysaccharides, 0.5%-2% of a cellulose derivative water-soluble polymer, 0.05%-2% of natural plant polysaccharides, 0.01%-1% of a natural water-soluble polymer of extracellular polysaccharides, 0.1%-2% of carbomer, 12%-40% of a wetting agent, 0.05%-4% of a pH regulating agent, 0.01%-2% of hyaluronic acid, 0.6%-3% of a transdermal absorption agent, 0.1%-0.3% of a penetration enhancer and the balance deionized water .The repairing gel has the excellent antibacterial effect and the good moisture absorption performance and can significantly increase the cure rate of a wound surface, shorten the healing time of the wound surface and prevent scar formation.
Owner:湖北兵兵药业(集团)有限公司

Growth factor porous micro-sphere compound system coated by injectable hydrogel

The invention relates to the preparation of a growth factor porous micro-sphere compound system coated by an injectable hydrogel. The preparation is characterized by comprising a first component, namely HA-ADH sol obtained by cross-linking of hyaluronic acid (HA) and adipic dihydrazide (ADH), and polylactic acid (PLLA) porous micro-spheres which are carried with growth factors and loaded in the first component. The compound system is capable of degrading and slowly releasing the growth factors under physiological conditions, so that the slow releasing of the growth factors in a body is realized, and the problems that the growth factors are released too fast and an action period is too short are solved, thereby reaching the effect of neural restoration.
Owner:WUHAN UNIV OF TECH

Acidic soil conditioner, preparation method and application thereof

The invention provides an acidic soil conditioner, a preparation method and application thereof. The acidic soil conditioner is formed by compounding of industrial waste, mineral raw materials and anSE functional material in certain proportion. The SE functional material comprises a glucoamylase treatment substance and amino acid biochar, as well as one or more of alginic acid, polyaspartic acid,polyglutamic acid and potassium fulvate. The acidic soil conditioner provided by the invention can effectively adjust the soil pH value, neutralize free acid, passivate activated aluminum, and solvethe problems of "acidity and toxicity" of soil; being rich in humic acid and organic matters, the acidic soil conditioner can effectively improve soil, restore the granular structure, increase the content of organic matters, and improve the water retention, fertility increasing and breathability of soil; the acidic soil conditioner can improve the rhizosphere soil microenvironment, enhance root activity, and promote nutrient uptake and utilization, also can improve the crop stress resistance and effectively prevent crop premature senescence, thus being a conditioner having the efficacy of improving soil and promoting crop growth.
Owner:CHINA AGRI UNIV

Method for producing feed microecological preparation by synergic fermentation of probiotics

The invention relates to a method for producing a feed microecological preparation by the synergic fermentation of probiotics, and the method can effectively solve the problems of low conversion ratio and production performance, weak disease resistance and strong toxic and side effects of feeds in the prior art. According to the technical scheme, bacillus subtilis, lactobacillus plantarum and candida are taken as bacterial strains and are inoculated and mixed evenly in a fermentation tank according to the inoculation quantity which is equal to 5%-10% of the volume of a culture medium, wherein the culture medium is prepared by mixing evenly 94%-96% of chrysanthemum meal, 0.8%-1.2% of Astragalus mongholicus, 0.8%-1.2% of forsythia, 0.8%-1.2% of Houttuynia cordata, 0.8%-1.2% of hawthorn and 0.8%-1.2% of pericarpium citri reticulatae by weight percentage and crushing the mixture to 60-100 meshsieves; oxygen ventilation fermentation is performed firstly, wherein the fermentation temperature is 30-35 DEG C, the ventilatory capacity is 0.5-1.2 L / min, the ventilation is stopped for 10 minutes every 30 minutes, the stirring speed is 45-60 r / min, and the culture lasts for 28-40 hours; then, anaerobic fermentation is performed, wherein the fermentation temperature is 32-35 DEG C, the stirring speed is 20-40 r / min, and the culture lasts for 24-48 hours; and finally, the feed microecological preparation is obtained. The feed microecological preparation can increase the utilization rate of protein and promote the growth of animals and is innovation of feed additives.
Owner:杨辉

Bacillus subtilis with phosphate-solubilizing effect and application thereof

The invention discloses bacillus subtilis with phosphate-solubilizing effect and application thereof, and relates to bacillus subtilis XF-8 (CCTCC No: M2010182) and a method for producing neutral phytase from the bacillus subtilis XF-8. The application comprises the following steps: inoculating a strain in an LB culture medium, and carrying out shake culture overnight at the temperature of 30 DEG C; inoculating the strain in an enzyme-producing culture medium with 1-5% of inoculation amount, and carrying out shake culture at the temperature of 25-37 DEG C for 2-4 days, wherein, the pH value is 5.5-7.5; centrifuging at the speed of 5000rpm at the temperature of 4 DEG for 15 minutes, and taking supernatant to obtain crude neutral phytase; and diluting the crude neutral phytase by 10 folds, and then measuring enzyme activity of fermentation liquor, wherein, the carbon source of the enzyme-producing culture medium is wheat bran, and the nitrogen source of the enzyme-producing culture medium comprises peptone from casein and (NH4)2SO4. The neutral phytase produced by the bacillus subtilis has high enzyme activity (pH=7.0), so the neutral phytase can be applied to development of fish feed for lowering phosphorus pollution in water, and can be also applied to development of biological bacterial fertilizer for effectively utilizing organic phosphorus in soil, reducing application amount of phosphate fertilizer, improving soil microenvironment and enhancing the quality of agricultural products.
Owner:NANKAI UNIV

Fertilizer for cultivation of tobacco, preparation method and application thereof

The invention provides a fertilizer for cultivation of tobacco. The fertilizer consists of the components by weight as follows: 0.5-1.5 parts of a composite microbial agent, 3-7 parts of an activating agent, 3-7 parts of binder, and 86-92 parts of nitrogen, phosphorus and potassium fertilizer. The effective components of the composite microbial agent are Bacillus licheniformis, Bacillus pumilus, Brevibacillus laterosporus, Bacillus megatherium and Silicate bacteria. The invention further provides a preparation method and an application of the fertilizer. The product provided by the invention can remarkably improve crumb-structure of soil for cultivation of tobacco, and microflora distribution and micro-environment of the soil. A plenty of derivatives are generated, thereby chelating or blocking damage of heavy metals, and absorption and assimilation of tobacco to nutrient elements are improved, the drought resisting and disease resisting abilities of tobacco are enhanced, damage of diseases and insects caused by continuous cropping of tobacco in successive years are effectively prevented, and in particular, tobacco mosaic virus and brown spot disease are specially prevented and treated. According to the invention, the raw materials are low in cost and easy to obtain and the preparation and using methods are simple and easy to operate.
Owner:HUNAN TAIGU BIOTECH

Efficient seed coating agent, preparation method and applications thereof, and method using same to process seeds

The invention belongs to the technical field of seed processing, and especially relates to an efficient seed coating agent, a preparation method and applications thereof, and a method using the same to process seeds. The efficient seed coating agent comprises the following components: amino-oligosaccharin, a dispersant, an adhesive, a surfactant, vigilance dye, and water. The preparation method comprises the following steps: weighing all raw materials other than amino-oligosaccharin according to a certain ratio, evenly mixing the weighed raw materials, then adding amino-oligosaccharin, and grinding the mixture until the particles of each component is less than 5 mm. The efficient seed coating agent is mixed with seeds according to a mass ratio of 1:50 to coat the seeds. The efficient seed coating agent can be used to prevent and treat diseases of fruit trees, vegetables, underground roots, tobaccos, traditional Chinese herbals, grain crops, cotton, and the like. The seed coating agent has the advantages of strong pertinence, good universality, sustain-released property, and fertilizer-retaining performance, and is capable of preventing the diseases and pests. Moreover, the raw materials are easily-available and cheap, so the production cost is low. Besides the raw materials are environment-friendly and harmless, and meet the nuisance-free requirements.
Owner:山东亿嘉农化有限公司
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