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627results about How to "Improve microenvironment" patented technology

Porket premix compound and porket creep feed prepared by the premix compound

The present invention provides a porket premix compound and a porket creep feed prepared by the porket premix compound. The porket premix compound is composed of the components of a yeast powder, an energy-nutrient accelerating agent, a flavoring agent, minor elements, etc. The porket creep feed is composed of the components of swelled corn flour, swelled soybean flour, swelled wheat flour, low-protein whey powder, porket premix compound, etc. The porket premix compound of the invention fully takes the electrolyte balance into consideration; supplies various minor elements and vitamins required by the porket, promotes the growth and is used for preparing the excellent proket creep feed. The general dosage is 1% of the weight of the porket creep feed. At least 85% of the porket creep feed passes through a 60 mesh sieve so that the granularity of the feed stuff is smaller and the absorption of the porket to the nutrition substance is facilitated. The porket creep feed further has the advantages of sustaining the integrality of the mucous membrane, overcoming the weaning stress, increasing the feed intake, increasing the digestion and absorption rate, reducing the feed-meat ratio, realizing the effect of continuous body weight growing after the weaning.
Owner:河南雄峰科技股份有限公司

Micro-nano composite dual-layer skin framework and manufacturing method thereof

The invention discloses a micro-nano composite dual-layer skin framework and a manufacturing method thereof which are applied to the skin regenerative tissue engineering. The manufacturing method includes the steps of firstly, preparing a nanofiber membrane layer by the electrospinning technique and utilizing the same as a surface layer of skin; secondly, preparing a three-dimensional framework by direct writing on the nanofiber membrane layer by an electrohydrodynamic inkjet printing method and utilizing the same as dermis. Specifically, the nanofiber membrane refers to a layer of nanofibers uniform in diameter; the nanofibers provide suitable surface morphology for cell adhesion and growth, thereby facilitating cell adhesion and growth; the three-dimensional framework refers to a three-dimension space composed of nanofibers by the electrohydrodynamic inkjet printing method, provides a microenvironment for growth of cells, and facilitates cell infiltration and proliferation; both the nanofiber membrane and the three-dimensional framework are made from biodegradable high-polymer material. Compared with the conventional artificial skin framework, the micro-nano composite dual-layer skin framework has the advantages of high plasticity, good mechanical property, three-dimension structure with high porosity, and high biocompatibility.
Owner:SHANGHAI UNIV

Preparation method for conduction and sustained release type nervous tissue engineering scaffold

The invention relates to a preparation method for a conduction and sustained release type nervous tissue engineering scaffold. The method comprises the following steps: silk fibroin Silk and lactic acid-caprolactone copolymer are dissolved in a solvent, dissolved and stirred to obtain a solution, then polyaniline and camphorsulfonic acid are added and stirred and mixed uniformly to obtain a cortex electrospinning solution, and NGF (nerve growth factors) are dissolved in ultrapure water completely to obtain a core electrospinning solution; and the cortex electrospinning solution and the core electrospinning solution are accommodated in an injector respectively for coaxial electrospinning, and then fumigation treatment and vacuum drying are performed to obtain the conduction type nervous tissue engineering scaffold. According to the prepared nanofiber scaffold, the nerve repair speed is increased from ways including external electrical stimulation (conductive polymer), biochemistry (NGFs), a topological structure required by nerve regeneration (orientation guide) and the like. The method is simple to operate, good in repeatability and high in economic benefit, and provides novel experimental thought for nerve defect repair in clinical application.
Owner:诺一迈尔(山东)医学科技有限公司

Combined fertilizer synergist containing polyglutamic acid, phosphate solubilizing bacteria and potassium bacteria and preparation method and application of combined fertilizer synergist

ActiveCN104276882AStrong ability to decompose inorganic phosphorusImprove bioavailabilityFertilizer mixturesPhosphate ionPhosphate solubilizing bacteria
The invention discloses a combined fertilizer synergist containing polyglutamic acid, phosphate solubilizing bacteria and potassium bacteria. The combined fertilizer synergist comprises the following components in proportion: phosphate solubilizing bacteria, potassium bacteria and polyglutamic acid at the mass ratio of (0.1-1) to (0.1-1) to (0.001-0.1). The invention also discloses a preparation method and application of the fertilizer synergist. The phosphate solubilizing bacteria disclosed by the invention are capable of effectively releasing phosphate anions (PO4<3+>) in soil or fertilizer; the potassium bacteria are capable of effectively releasing potassium ions (K<+>) in the soil or fertilizer; after being compounded with the phosphate solubilizing bacteria and the potassium bacteria, the polyglutamic acid not only can provide a good growth environment for survival of the phosphate solubilizing bacteria and the potassium bacteria, but also can fully chelate nutrient elements released by the phosphate solubilizing bacteria and the potassium bacteria; and due to the synergistic effect, the effect of solubilizing phosphate and potassium is relatively outstanding, therefore the combined fertilizer synergist can be cooperatively used together with fertilizers, the utilization rate of the fertilizer is increased, and the combined fertilizer synergist can also be directly fertilized as a bacterial manure.
Owner:轩凯生物科技(滁州)有限公司

Thermosensitive dual-administration nanocomposite hydrogel as well as preparation method and application thereof

The invention belongs to the technical fields of biomedical materials and medical apparatus, and in particular relates to a thermosensitive dual-administration nanocomposite hydrogel as well as a preparation method and application thereof. The preparation method of the thermosensitive dual-administration nanocomposite hydrogel comprises the following steps: preparing a poly (N-isopropylacrylamide) nano microsphere and loading a growth factor, preparing a poly (N-isopropylacrylamide-co-acrylic acid) nano microsphere and loading an anti-inflammatory drug, and preparing the thermosensitive dual-administration nanocomposite hydrogel. The finished product takes sodium alginate as a matrix phase and uses two poly (N-isopropylacrylamide) based thermosensitive nano gel microspheres which have different LCSTs and are respectively loaded with the anti-inflammatory drug and the growth factor as a dispersion phase; and by controlling the temperature of the composite hydrogel to be higher than or lower than LCST of the nano microspheres, the release of the anti-inflammatory drug and the release of the growth factor are controlled, so that controlled release of the anti-inflammatory drug and the growth factor in inflammatory stage and proliferative stage of a deep wound repairing process is achieved.
Owner:JINAN UNIVERSITY

Method for producing feed microecological preparation by synergic fermentation of probiotics

The invention relates to a method for producing a feed microecological preparation by the synergic fermentation of probiotics, and the method can effectively solve the problems of low conversion ratio and production performance, weak disease resistance and strong toxic and side effects of feeds in the prior art. According to the technical scheme, bacillus subtilis, lactobacillus plantarum and candida are taken as bacterial strains and are inoculated and mixed evenly in a fermentation tank according to the inoculation quantity which is equal to 5%-10% of the volume of a culture medium, wherein the culture medium is prepared by mixing evenly 94%-96% of chrysanthemum meal, 0.8%-1.2% of Astragalus mongholicus, 0.8%-1.2% of forsythia, 0.8%-1.2% of Houttuynia cordata, 0.8%-1.2% of hawthorn and 0.8%-1.2% of pericarpium citri reticulatae by weight percentage and crushing the mixture to 60-100 meshsieves; oxygen ventilation fermentation is performed firstly, wherein the fermentation temperature is 30-35 DEG C, the ventilatory capacity is 0.5-1.2 L/min, the ventilation is stopped for 10 minutes every 30 minutes, the stirring speed is 45-60 r/min, and the culture lasts for 28-40 hours; then, anaerobic fermentation is performed, wherein the fermentation temperature is 32-35 DEG C, the stirring speed is 20-40 r/min, and the culture lasts for 24-48 hours; and finally, the feed microecological preparation is obtained. The feed microecological preparation can increase the utilization rate of protein and promote the growth of animals and is innovation of feed additives.
Owner:杨辉

Bacillus subtilis with phosphate-solubilizing effect and application thereof

The invention discloses bacillus subtilis with phosphate-solubilizing effect and application thereof, and relates to bacillus subtilis XF-8 (CCTCC No: M2010182) and a method for producing neutral phytase from the bacillus subtilis XF-8. The application comprises the following steps: inoculating a strain in an LB culture medium, and carrying out shake culture overnight at the temperature of 30 DEG C; inoculating the strain in an enzyme-producing culture medium with 1-5% of inoculation amount, and carrying out shake culture at the temperature of 25-37 DEG C for 2-4 days, wherein, the pH value is 5.5-7.5; centrifuging at the speed of 5000rpm at the temperature of 4 DEG for 15 minutes, and taking supernatant to obtain crude neutral phytase; and diluting the crude neutral phytase by 10 folds, and then measuring enzyme activity of fermentation liquor, wherein, the carbon source of the enzyme-producing culture medium is wheat bran, and the nitrogen source of the enzyme-producing culture medium comprises peptone from casein and (NH4)2SO4. The neutral phytase produced by the bacillus subtilis has high enzyme activity (pH=7.0), so the neutral phytase can be applied to development of fish feed for lowering phosphorus pollution in water, and can be also applied to development of biological bacterial fertilizer for effectively utilizing organic phosphorus in soil, reducing application amount of phosphate fertilizer, improving soil microenvironment and enhancing the quality of agricultural products.
Owner:NANKAI UNIV

Fertilizer for cultivation of tobacco, preparation method and application thereof

The invention provides a fertilizer for cultivation of tobacco. The fertilizer consists of the components by weight as follows: 0.5-1.5 parts of a composite microbial agent, 3-7 parts of an activating agent, 3-7 parts of binder, and 86-92 parts of nitrogen, phosphorus and potassium fertilizer. The effective components of the composite microbial agent are Bacillus licheniformis, Bacillus pumilus, Brevibacillus laterosporus, Bacillus megatherium and Silicate bacteria. The invention further provides a preparation method and an application of the fertilizer. The product provided by the invention can remarkably improve crumb-structure of soil for cultivation of tobacco, and microflora distribution and micro-environment of the soil. A plenty of derivatives are generated, thereby chelating or blocking damage of heavy metals, and absorption and assimilation of tobacco to nutrient elements are improved, the drought resisting and disease resisting abilities of tobacco are enhanced, damage of diseases and insects caused by continuous cropping of tobacco in successive years are effectively prevented, and in particular, tobacco mosaic virus and brown spot disease are specially prevented and treated. According to the invention, the raw materials are low in cost and easy to obtain and the preparation and using methods are simple and easy to operate.
Owner:HUNAN TAIGU BIOTECH

Efficient seed coating agent, preparation method and applications thereof, and method using same to process seeds

The invention belongs to the technical field of seed processing, and especially relates to an efficient seed coating agent, a preparation method and applications thereof, and a method using the same to process seeds. The efficient seed coating agent comprises the following components: amino-oligosaccharin, a dispersant, an adhesive, a surfactant, vigilance dye, and water. The preparation method comprises the following steps: weighing all raw materials other than amino-oligosaccharin according to a certain ratio, evenly mixing the weighed raw materials, then adding amino-oligosaccharin, and grinding the mixture until the particles of each component is less than 5 mm. The efficient seed coating agent is mixed with seeds according to a mass ratio of 1:50 to coat the seeds. The efficient seed coating agent can be used to prevent and treat diseases of fruit trees, vegetables, underground roots, tobaccos, traditional Chinese herbals, grain crops, cotton, and the like. The seed coating agent has the advantages of strong pertinence, good universality, sustain-released property, and fertilizer-retaining performance, and is capable of preventing the diseases and pests. Moreover, the raw materials are easily-available and cheap, so the production cost is low. Besides the raw materials are environment-friendly and harmless, and meet the nuisance-free requirements.
Owner:山东亿嘉农化有限公司
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