75 results about "Acellular matrix" patented technology

The invention discloses a preparation method of an acellular matrix containing a bioactive coating, and belongs to the field of medical beauty industry, the key point of the technical scheme is that the preparation method comprises the preparation steps of S1, preparation of the acellular matrix; s2, treating the acellular matrix; s3, rehydrating the decellularized matrix, sequentially immersing the rehydrated decellularized matrix into an activating solution, a collagen solution and an activating solution, reacting, washing the collagen matrix, and freeze-drying to obtain the collagen matrix; s4, adding the anti-inflammatory microcapsules and the growth factor nano-carrier into the hyaluronic acid solution to obtain a mixed solution; s5, the collagen matrix is immersed in an activating solution, after reaction and washing, the surface of the collagen matrix is coated with the mixed solution for reaction, after curing, washing and drying are conducted, and the acellular matrix containing the bioactive coating is obtained.The covalently-connected hyaluronic acid-collagen double-layer structure is constructed on the surface of the acellular matrix, so that biocompatibility and safety are improved; meanwhile, the problems that a traditional acellular matrix is unstable in structure and poor in mechanical strength are solved.

The invention belongs to the technical field of medical biological materials, and particularly relates to a sodium hyaluronate gel regeneration filler for injection and a preparation method and application thereof.The sodium hyaluronate gel serves as a carrier to disperse acellular matrix particles, the sodium hyaluronate gel forms a micron-sized porous structure in the cross-linking process through a microbubbling technology, and the sodium hyaluronate gel is prepared into the sodium hyaluronate gel regeneration filler for injection and the preparation method and application of the sodium hyaluronate gel regeneration filler. A local cross-linked network is strengthened by combining a secondary cross-linking process, high elastic modulus is realized while the content of sodium hyaluronate is reduced, so that the porous structure has excellent instant filling and shaping capability and controllable degradation behavior, the porous structure can promote permeation of hyaluronidase, the degradation period of the hyaluronidase is matched with the tissue regeneration process, and the tissue regeneration effect is improved. And the acellular matrix particles can continuously induce adhesion, migration, proliferation and differentiation of endogenous cells of tissues, synergistically promote in-situ tissue regeneration, effectively reduce repeated injection requirements and reduce long-term complication risks, and have a good application prospect in soft tissue filling and repairing.

The invention provides a self-assembled temperature-sensitive hydrogel for neural restoration and a preparation method thereof. The hydrogel comprises the following raw materials: poloxamer, an acellular matrix and a neural factor in a mass concentration ratio of (5-10): (10-20): (1-4), preferably 5: 15: 1. The preparation method comprises the following steps: respectively preparing a poloxamer solution and an acellular matrix solution, mixing and heating the poloxamer solution and the acellular matrix solution to form a double-network three-dimensional gel structure, adding the nerve factors, stirring or ultrasonically dispersing, and heating to form the bioactive hydrogel. The hydrogel can rapidly form a stable three-dimensional structure at the body temperature, a support is provided for nerve repair, cell adhesion and proliferation are supported, and the added nerve factors are beneficial to maintaining the activity of neurons. Meanwhile, the hydrogel is in a liquid state at a low temperature, is convenient to inject, is fixed at a body temperature, is adjustable in degradation rate, is non-toxic, is suitable for local nerve injury treatment, and improves the bioavailability and safety of a nerve repair material.

The invention provides an in-situ tissue engineering valve stent material as well as a preparation method and application thereof, and belongs to the technical field of medical materials. According to the invention, the surface of the acellular matrix is loaded with the super-particle coating formed by self-assembly of the polyphenol-mediated polypeptide aggregate and the Prussian blue nano-enzyme, so that the stent material is endowed with double ROS regulation and endothelial cell adhesion promoting functions, excessive deposition of collagenous fibers can be prevented, and fibrosis of valve tissues is avoided; the synergistic effect of the components of the super-particle coating promotes the endothelialization process of the stent and the remodeling of a functional extracellular matrix, so that the accelerated formation of the functional endodermis is finally realized, and a powerful guarantee is provided for the success of clinical transformation of an in-situ heart valve tissue engineering technology.

The invention discloses an implantable acellular matrix material and a preparation method thereof, and belongs to the field of biomedical materials. According to the method, natural animal tissues (such as pericardium, achilles tendon or small intestine submucosa) are used as raw materials, a low-temperature chemical combined decellularization treatment technology is adopted, and the method comprises the multi-step synergistic process of pre-cooling soaking balancing, low-temperature enzymolysis, low-concentration surfactant gradient permeation cleaning, low-temperature nuclease degradation, non-crosslinking sterilization and the like. Under the condition that the treatment temperature is strictly kept at 0-8 DEG C, cell components and genetic materials (the residual DNA content is 1t, and the dry weight is 8 ng / mg) are efficiently removed, and meanwhile the natural three-dimensional fine structure of an extracellular matrix (ECM), the integrity of collagen fibrils and key bioactive components (such as glycosaminoglycans (GAGs)) are reserved to the maximum extent. The host immunological rejection reaction of the obtained material is remarkably reduced, host cell ingrowth, vascularization and tissue function reconstruction can be effectively promoted after implantation, and the material is suitable for high-end implantation scenes such as soft tissue repair and regenerative medical stents.

An acellular matrix woven material, a preparation method and an application thereof are provided. The acellular matrix woven material is prepared by combining 3D printing with weaving. At the same time, also provided are the acellular matrix woven material prepared by the preparation method and its application in preparing tissue engineering scaffold materials.

The invention provides acellular matrix hydrogel loaded with animal acellular omentum particles as well as a preparation method and application of the acellular matrix hydrogel loaded with the animal acellular omentum particles, and relates to the technical field of tissue engineering and regenerative medicine. Comprising a decellularization treatment agent, a decellularization matrix, functional particles, an enzymolysis agent, a cross-linking stabilizer, a functional additive and a solvent, the functional additive is prepared from PDA coated Fe3O4 magnetic nano particles, phosphorylated polyglycerol sebacate and liposome coated silver nano particles. The PDA-Fe3O4 magnetic nanoparticles of the functional additive generate mechanical signals under the action of an external magnetic field, a Piezo1 mechanical gating cation channel is activated to promote calcium ion internal flow, and the autonomous mineralization ability of phosphorylated polyglycerol sebacate is combined, so that the activity of the bone differentiation marker alkaline phosphatase is effectively improved, and the bone differentiation marker alkaline phosphatase is prepared. The combination of the PDA coated Fe3O4 magnetic nanoparticles and the phosphorylated polyglycerol sebacate integrates a magnetic response mineralization system.

The invention discloses an antibacterial composite periosteum for an oral cavity and a processing technology of the antibacterial composite periosteum, and relates to the technical field of periosteum. The method specifically comprises the following steps: step 1, treating defatted pig peritoneum with a decellularized solution, cleaning and sterilizing to obtain a pig peritoneum decellularized matrix; 2, treating the pig peritoneal decellularized matrix with a glyoxal solution, cleaning, and sterilizing to obtain a pretreated pig peritoneal decellularized matrix; step 3, uniformly coating the surface of the pretreated porcine peritoneal acellular matrix with an antibacterial solution, and performing sterilization, standing and freeze-drying to obtain the antibacterial composite periosteum. Wherein the antibacterial solution is prepared from the following raw material components in concentration: 1 to 2 weight percent of modified chitosan, 0.2 to 0.4 weight percent of amino-terminated polyethylene glycol and 0.01 to 0.05 weight percent of p-toluenesulfonic acid, and a solvent is a PBS (Phosphate Buffer Solution). The antibacterial composite periosteum is good in biocompatibility and has excellent clinical application value in oral surgery.

A microneedle loaded with at least curcumin, exosomes, and an acellular matrix. Curcumin is incorporated into the exosomes to form functionalized exosomes (Cur@EV), which serve as the primary active ingredient for the repair of hypertrophic scars. Cur@EV has been shown to exhibit a high affinity for hypertrophic scar fibroblasts, while more efficient delivery of curcumin to HSFs has demonstrated enhanced anti-HSF cell proliferation activity and significant anti-fibrosis effects, potentially meeting clinical needs for the repair of hypertrophic scars.

The application discloses a kind of tissue regeneration fillers and preparation method and application thereof, belong to the field of biomedical materials and medical cosmetology technical field. Including the following mass fraction components: mixed microparticle 1.0~5.0 parts;Mixed microparticle includes acellular matrix microparticle and basement membrane microparticle, and the mass ratio of acellular matrix microparticle and basement membrane microparticle is 10‑1:1.The application extracts and compounding two kinds of microparticles respectively, combines gel matrix and dispersion liquid processing, retains the scaffold function of acellular matrix, supplements basement membrane specific active ingredient, realizes instant support and long-acting regeneration synergy;Filler has excellent injectability and biocompatibility, can accurately repair basement membrane structure degradation, promote cell directional migration and tissue functional regeneration, and is widely used in facial rejuvenation, wound repair and soft tissue regeneration.

The invention discloses a composite artificial cavity / membrane tissue and a preparation method thereof. The composite artificial cavity / membrane tissue is formed by compounding a synthetic high-molecular material and a natural high-molecular biological material, and the synthetic high-molecular material is selected from more than one of silicone, synthetic high-molecular hydrogel and an acrylic polymer. The natural polymer biological material is selected from more than one of an acellular matrix scaffold material, a non-acellular matrix and non-acellular collagen. The composite artificial cavity / membrane tissue provided by the invention has the advantages of good healing after transplantation, high biomechanical strength, low immunogenicity, smooth surface and the like.

The invention relates to the technical field of artificial dermis, in particular to a preparation method and application of artificial dermis based on bionic acellular matrix. The artificial dermis comprises a bionic acellular matrix component A and an auxiliary material component B, wherein the component A comprises type I collagen, type VI collagen, elastin, fibronectin, laminin, mother cell protein and glucan; the component B comprises antibacterial peptide, antibiotics and a high-molecular polymer material; the mass ratio of the component A to the component B is (4-6): (1-4). The preparation method comprises the following steps: respectively preparing procedure mother liquor of the component A and the component B, preparing hydrogel with a double-layer gradient structure through step-by-step crosslinking, and then carrying out freeze drying and sterilization treatment. The artificial dermis disclosed by the invention has good biocompatibility and antibacterial property, can effectively promote wound healing, is suitable for repairing acute and chronic wounds of four limbs, faces and trunk parts, and is particularly suitable for repairing complex wounds with exposed bones, tendons, nerves and blood vessels.

The application provides a kind of two-component multifunctional nanometer membrane and its preparation method and application, it is related to biological medical material technical field, the nanometer membrane, by including the following mass fraction of component is prepared: high molecular matrix component one 50~75%, high molecular matrix component two 24~49%, functional component 1%;The high molecular matrix component one is hyaluronic acid or hyaluronic acid derivative, the high molecular matrix component two is one of collagen, gelatin, methacrylated gelatin or acellular matrix, the functional component includes drug and active factor;The drug is loaded on high molecular matrix component one, and the active factor is loaded on high molecular matrix component two;The nanometer membrane is formed by chemical crosslinking.This application overcomes the shortcomings and deficiencies of existing corneal alkali burn and infection treatment means, provides a kind of two-component multifunctional nanometer membrane with good biological safety, effective control infection and inhibits inflammatory response, for treating corneal injury.

The invention relates to the technical field of medical biological materials, in particular to a modified acellular matrix. According to the method, microcrystal drying, immobilization and swelling treatment are combined in sequence in the decellularization treatment process, so that the phenomenon of low porosity after crosslinking can be effectively reduced, meanwhile, the mechanical loss in the decellularization process is reduced, and the high mechanical property is considered while the high porosity is obtained. According to the modified acellular matrix, the defect that the mechanical property of an acellular matrix material with the unit thickness is insufficient is remarkably overcome, and the modified acellular matrix has high porosity and high mechanical property at the same time. The acellular matrix is thin and tough, the comfort degree is high after implantation, the tensile strength is improved to more than three times that of an original material, and the acellular matrix has extremely high application value in the medical field with requirements for mechanical property, porosity, thickness and comfort degree.

The present application belongs to the field of biomedical materials and tissue engineering, and particularly relates to a kind of acellular matrix aerogel repair material and its preparation method and application.Further, the present application relates to the comprehensive preparation method of the material, especially the extremely low immunogenicity acellular process combined with physical, chemical and supercritical fluid technologies, and the unique aerogel forming technology based on supercritical drying.Further, the present application relates to the application of the material, mainly related to soft tissue repair, medical cosmetology, bone / cartilage injury repair field.

The application relates to the technical field of biomaterials, in particular to a crosslinked amniotic membrane acellular matrix filling material and a preparation method thereof. The crosslinked amniotic membrane acellular matrix filling material is prepared by using a photo-crosslinking technology, and the specific preparation method is as follows: placing an acellular amniotic membrane matrix in a pretreatment agent phosphate buffer solution to perform pretreatment, then performing functionalization treatment on the acellular amniotic membrane extracellular matrix, preparing a photo-crosslinking solution, immersing the treated acellular amniotic membrane extracellular matrix in the photo-crosslinking solution, permeating at room temperature, and finally performing irradiation under a UV light source to form a stable three-dimensional network structure. The material has high mechanical strength, controllable degradability and excellent biocompatibility, and is suitable for tissue repair and regenerative medicine.

The application relates to the technical field of tissue repair, in particular to an amniotic membrane acellular matrix for filling material and a preparation method. The amniotic membrane acellular matrix with a tissue repair function is prepared from amniotic membrane tissue, and the preparation method of the amniotic membrane acellular matrix adopts specific processes such as enzymolysis, so that the content of growth factors, collagen, glycosaminoglycan and other components in the amniotic membrane acellular matrix can be effectively improved, different types of tissue repair functions can be met, the amniotic membrane acellular matrix is suitable for being used as a personalized biological filling material, and has a wide application prospect.

The application relates to the field of biomaterials, and particularly discloses a kind of acellular matrix material and a preparation method thereof.The preparation method of the material comprises the following steps: extracting tissue material of a mammal, performing acellular treatment on the tissue material to obtain acellular tissue material, freeze-drying the tissue material, and then crushing the freeze-dried tissue material to obtain animal-derived acellular matrix; the animal-derived acellular matrix is resuspended in water, mixed with a modified epoxidized chitosan solution, and then transglutaminase is added to perform a reaction; the product obtained is freeze-dried to obtain the acellular matrix material.The method can improve the biocompatibility of the acellular matrix and reduce the immune rejection reaction by cross-linking the animal-derived acellular matrix with modified epoxidized chitosan; at the same time, since the cross-linking reaction is a biological cross-linking reaction under the action of transglutaminase, the biological activity factors contained in the acellular matrix are not easily destroyed during the cross-linking process, and the biological activity and safety are better.

The invention relates to the technical field of biological materials, in particular to a preparation method and application of a nucleus pulposus acellular matrix hydrogel material. The method comprises the following steps: taking a proper amount of lumbar nucleus pulposus tissues of pigs in a slaughter house, cleaning the lumbar nucleus pulposus tissues with PBS for several times, then taking part of the lumbar nucleus pulposus tissues for treatment, and subpackaging the residual nucleus pulposus tissues at-20 DEG C Preparing a decellularized liquid working solution; preparing an acellular nucleus pulposus matrix; cleaning the acellular nucleus pulposus matrix; evaluating acellular nucleus pulposus matrix genetic material residues and extracellular matrix preservation conditions; preparing a powdery nucleus pulposus acellular matrix; preparing a methacrylated acellular nucleus pulposus matrix; detecting the cross-linking performance of the methacrylated acellular nucleus pulposus matrix; through the mode, the efficient and complete nucleus pulposus acellular matrix can be obtained, and the nucleus pulposus acellular matrix hydrogel material which has good activity, proper mechanical property, quick crosslinking, injectability and strong applicability can be obtained.

The application discloses an animal small intestinal submucosa acellular matrix anti-adhesion membrane and a preparation method and application thereof, and belongs to the field of biological materials. The animal small intestinal submucosa acellular matrix anti-adhesion membrane is obtained by sterilizing one side of the animal small intestinal submucosa acellular matrix after laser treatment. The animal small intestinal submucosa acellular matrix anti-adhesion membrane has reduced thickness, improved degradation performance, can guide postoperative wound tissue regeneration, can prevent other tissues from adhering to a wound surface, and is particularly suitable for a case that the degradation rate of a sheet material needs to be further improved, and has a wide application prospect in preparation of anti-adhesion tissue repair materials.

The utility model discloses a sterilization box for an acellular matrix, and belongs to the field of sterilization boxes. The device mainly comprises a box assembly, the box assembly comprises a box body, a cavity is formed in the box body, articles can be located in the cavity, a supporting assembly is fixedly installed at the position of the cavity in the box assembly, the supporting assembly comprises a supporting table, and the supporting table can place the articles needing to be detected in a centralized mode; according to the sterilization box for the acellular matrix, by arranging the supporting table, articles needing to be sterilized can be placed in the box body in a centralized mode, and the supporting table can be placed on the multiple sets of fixing blocks, so that the supporting table can be supported and limited in the box body through the fixing blocks; and meanwhile, through cooperation between a spring and a lower pressing plate, the supporting table can be clamped when being placed on a fixing block, so that the stability of the supporting table can be guaranteed, and the situation that the supporting table easily shakes by a large margin in the box body can be prevented.

The invention belongs to the technical field of biological tissue engineering, and discloses a fat acellular extracellular matrix hydrogel preparation method, which comprises: (1) cutting cleaned fresh fat into pieces, and repeatedly freezing and thawing with a hypertonic salt solution; (2) homogenizing the frozen and thawed fat at low temperature, and collecting and precipitating; (3) re-suspending the precipitate by using PBS, oscillating in a shaking table, centrifuging at room temperature, and collecting the precipitate again; (4) carrying out degreasing and decellularization treatment on the collected precipitate by using a composite eluent; and (5) washing the precipitate with isopropanol for 24 hours, finally repeatedly washing the precipitate with distilled water, and collecting the precipitate, namely the fat acellular matrix. And (6) digesting the fat extracellular matrix with pepsin hydrochloride, and adjusting the pH value to be neutral with sodium hydroxide to obtain the fat extracellular matrix hydrogel. According to the preparation method of the fat extracellular matrix hydrogel, biological enzyme and high-speed centrifugation are not needed in the decellularization stage, the technology is simple, time consumption is short, the yield is high, the fat extracellular matrix prepared through the method is easy to digest, and the prepared hydrogel solution is uniform, good in injectability and capable of being used for production of a filling material for injection.

The invention belongs to the field of biological materials and tissue engineering, and particularly relates to an acellular matrix artificial blood vessel or membrane material for achieving inflammation regulation and a preparation method of the acellular matrix artificial blood vessel or membrane material. The preparation method comprises the following steps: 1) modifying alkynyl molecules on the surface of an acellular matrix artificial blood vessel or a membrane material; 2) modifying an azide group on the surface of the stem cell; and 3) carrying out mixed culture on the stem cells of which the surfaces are modified by the azide groups obtained in the step 2) and the acellular matrix artificial blood vessel or membrane material of which the surfaces are modified by the alkynyl molecules obtained in the step 1) to obtain the acellular matrix artificial blood vessel or membrane material for realizing inflammation regulation. According to the technical scheme, the biological orthogonal reaction is used for mediating rapid and stable combination between the surface modified artificial blood vessel or membrane material and the azide group labeled stem cells, so that the acellular matrix artificial blood vessel / membrane material stably loads the stem cells before transplantation; after being implanted into a body, the artificial blood vessel graft becomes an ideal anti-inflammatory and regeneration-promoting artificial blood vessel graft.

The invention provides an acellular matrix fiber woven scaffold as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The acellular matrix fiber woven scaffold comprises a woven structure unit, wherein the woven structure unit comprises warp yarns and a plurality of acellular matrix fiber weft yarns; the acellular matrix fiber is prepared by cutting an acellular matrix paper-based material at equal intervals; the plurality of acellular matrix fiber wefts are arranged in parallel according to a bionic trend and are interwoven and fixed through the warps, so that a bionic pattern simulating natural tissue fiber arrangement is formed in the weaving structure unit. The acellular matrix fiber scaffold is prepared by innovatively applying a weaving technology, the scaffold can improve the cell loading efficiency, is beneficial to self-repair and reconstruction of damaged tissues, provides suitable survival and growth conditions for cells, and has good application prospects in the field of biomedical engineering, especially in the aspects of tissue repair and regenerative medicine. And huge application potential is shown.

The invention provides a self-supporting acellular matrix amniotic membrane composite material with an interpenetrating network structure as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The preparation method of the composite material comprises the following steps: immersing an acellular matrix amnion into a polyfunctional group polymer solution, then immersing into a cross-linking agent solution for cross-linking to obtain gel, and drying the gel to obtain the composite material. The obtained composite material has an interpenetrating network structure, and compared with an acellular matrix amniotic membrane, the mechanical property is remarkably improved.

The present application relates to a composition comprising a cross-linked hyaluronic acid gel and acellular matrix microparticles wherein the gel particles have a porous structure, the median of the pore diameter is between 50 and 1000 [mu] m, and the acellular matrix microparticles are derived from animal-derived and human-derived tissues and organs, the acellular matrix particles are preferably selected from one or a mixture of skin, pericardium, peritoneum, mucous membrane, peritoneum, tendon, muscle, bone and cartilage, and the median of the particle size of the acellular matrix particles is 5-300 microns, preferably 10-200 microns, more preferably 20-100 microns and even 30-50 microns.

The invention discloses an oral squamous cell carcinoma decellularized matrix and a preparation method of dECM hydrogel, and the preparation method of the oral squamous cell carcinoma decellularized matrix comprises the following steps: soaking the tissue in a 0.1% w / v SDS solution for 28 hours, magnetically stirring at room temperature, and replacing the SDS solution every 12 hours; deionized water is used for cleaning for at least one hour to remove SDS residual liquid; soaking and eluting residual nucleic acid fragments by using 40U / mL nuclease, and magnetically stirring at 37 DEG C for 3 hours; and washing with deionized water for at least 1 hour, and storing the obtained oral squamous cell carcinoma acellular matrix at-80 DEG C for later use. The invention also discloses application of the dECM hydrogel in preparation of a three-dimensional cell culture system and in-vitro biological models with different invasion abilities.

Acellular substrates that provide continuous 3D growth of a variety of cells and / or tissues and methods of making the same are provided. The acellular substrates have a three- dimensional (3D) macrostructure defined by a continuous matrix of extracellular matrix (ECM) material associated with a first cell type of interest and a network of microporous channels and / or chambers extending throughout the continuous matrix of ECM material associated with the first cell type of interest. Methods of forming a personalized graft using a patient's own cells is also provided.

The invention discloses application of an acellular matrix material as a tissue damage or tumor targeting probe or carrier. A preparation method of the acellular matrix material comprises the following steps: pretreating fish tissues; performing decellularization treatment on the pretreated fish tissue to obtain a decellularized fish tissue material; the preparation method comprises the following steps: freeze-drying, grinding and digesting the decellularized fish tissue material, then carrying out ultrafiltration and centrifugation to intercept filtrate with the molecular weight of less than 50KDa, and freeze-drying to obtain the decellularized matrix material. The acellular matrix material disclosed by the invention has good blood compatibility and has a targeting effect on tissue injury, especially myocardial injury parts, diabetic nephropathy tissues or tumors.

The invention provides an acellular matrix for treating stress urinary incontinence as well as a preparation method and application of the acellular matrix. Splitting the soft tissue by using a cell lysis solution to remove cell debris; removing nucleic acid, degreasing and then micronizing; and adding the soft tissue particles and the soft tissue cross-linked particles into a phosphate buffer solution according to a ratio, mixing and emulsifying to obtain the acellular matrix for injection for treating stress urinary incontinence. The cell removal rate reaches 99%, the fat residue is less than 0.3%, and the injection is injected around the sphincter of the urethra (middle section or bladder neck) and / or in the urethra according to 0.5 ml / point and 3-4 points during clinical application to play roles in filling, connecting and repairing tissues, so that the damaged urethra supporting structure is repaired, the structural integrity and strength of connective tissues and the sphincter around the urethra are maintained, and the application prospect is wide. Therefore, the symptom of stress urinary incontinence is improved.