55 results about "Acellular matrix" patented technology

The invention belongs to the technical field of medical biological materials, and particularly relates to a sodium hyaluronate gel regeneration filler for injection and a preparation method and application thereof.The sodium hyaluronate gel serves as a carrier to disperse acellular matrix particles, the sodium hyaluronate gel forms a micron-sized porous structure in the cross-linking process through a microbubbling technology, and the sodium hyaluronate gel is prepared into the sodium hyaluronate gel regeneration filler for injection and the preparation method and application of the sodium hyaluronate gel regeneration filler. A local cross-linked network is strengthened by combining a secondary cross-linking process, high elastic modulus is realized while the content of sodium hyaluronate is reduced, so that the porous structure has excellent instant filling and shaping capability and controllable degradation behavior, the porous structure can promote permeation of hyaluronidase, the degradation period of the hyaluronidase is matched with the tissue regeneration process, and the tissue regeneration effect is improved. And the acellular matrix particles can continuously induce adhesion, migration, proliferation and differentiation of endogenous cells of tissues, synergistically promote in-situ tissue regeneration, effectively reduce repeated injection requirements and reduce long-term complication risks, and have a good application prospect in soft tissue filling and repairing.

The invention provides an in-situ tissue engineering valve stent material as well as a preparation method and application thereof, and belongs to the technical field of medical materials. According to the invention, the surface of the acellular matrix is loaded with the super-particle coating formed by self-assembly of the polyphenol-mediated polypeptide aggregate and the Prussian blue nano-enzyme, so that the stent material is endowed with double ROS regulation and endothelial cell adhesion promoting functions, excessive deposition of collagenous fibers can be prevented, and fibrosis of valve tissues is avoided; the synergistic effect of the components of the super-particle coating promotes the endothelialization process of the stent and the remodeling of a functional extracellular matrix, so that the accelerated formation of the functional endodermis is finally realized, and a powerful guarantee is provided for the success of clinical transformation of an in-situ heart valve tissue engineering technology.

The invention provides acellular matrix hydrogel loaded with animal acellular omentum particles as well as a preparation method and application of the acellular matrix hydrogel loaded with the animal acellular omentum particles, and relates to the technical field of tissue engineering and regenerative medicine. Comprising a decellularization treatment agent, a decellularization matrix, functional particles, an enzymolysis agent, a cross-linking stabilizer, a functional additive and a solvent, the functional additive is prepared from PDA coated Fe3O4 magnetic nano particles, phosphorylated polyglycerol sebacate and liposome coated silver nano particles. The PDA-Fe3O4 magnetic nanoparticles of the functional additive generate mechanical signals under the action of an external magnetic field, a Piezo1 mechanical gating cation channel is activated to promote calcium ion internal flow, and the autonomous mineralization ability of phosphorylated polyglycerol sebacate is combined, so that the activity of the bone differentiation marker alkaline phosphatase is effectively improved, and the bone differentiation marker alkaline phosphatase is prepared. The combination of the PDA coated Fe3O4 magnetic nanoparticles and the phosphorylated polyglycerol sebacate integrates a magnetic response mineralization system.

The invention discloses an antibacterial composite periosteum for an oral cavity and a processing technology of the antibacterial composite periosteum, and relates to the technical field of periosteum. The method specifically comprises the following steps: step 1, treating defatted pig peritoneum with a decellularized solution, cleaning and sterilizing to obtain a pig peritoneum decellularized matrix; 2, treating the pig peritoneal decellularized matrix with a glyoxal solution, cleaning, and sterilizing to obtain a pretreated pig peritoneal decellularized matrix; step 3, uniformly coating the surface of the pretreated porcine peritoneal acellular matrix with an antibacterial solution, and performing sterilization, standing and freeze-drying to obtain the antibacterial composite periosteum. Wherein the antibacterial solution is prepared from the following raw material components in concentration: 1 to 2 weight percent of modified chitosan, 0.2 to 0.4 weight percent of amino-terminated polyethylene glycol and 0.01 to 0.05 weight percent of p-toluenesulfonic acid, and a solvent is a PBS (Phosphate Buffer Solution). The antibacterial composite periosteum is good in biocompatibility and has excellent clinical application value in oral surgery.

The application discloses a kind of tissue regeneration fillers and preparation method and application thereof, belong to the field of biomedical materials and medical cosmetology technical field. Including the following mass fraction components: mixed microparticle 1.0~5.0 parts;Mixed microparticle includes acellular matrix microparticle and basement membrane microparticle, and the mass ratio of acellular matrix microparticle and basement membrane microparticle is 10‑1:1.The application extracts and compounding two kinds of microparticles respectively, combines gel matrix and dispersion liquid processing, retains the scaffold function of acellular matrix, supplements basement membrane specific active ingredient, realizes instant support and long-acting regeneration synergy;Filler has excellent injectability and biocompatibility, can accurately repair basement membrane structure degradation, promote cell directional migration and tissue functional regeneration, and is widely used in facial rejuvenation, wound repair and soft tissue regeneration.

The invention discloses a composite artificial cavity / membrane tissue and a preparation method thereof. The composite artificial cavity / membrane tissue is formed by compounding a synthetic high-molecular material and a natural high-molecular biological material, and the synthetic high-molecular material is selected from more than one of silicone, synthetic high-molecular hydrogel and an acrylic polymer. The natural polymer biological material is selected from more than one of an acellular matrix scaffold material, a non-acellular matrix and non-acellular collagen. The composite artificial cavity / membrane tissue provided by the invention has the advantages of good healing after transplantation, high biomechanical strength, low immunogenicity, smooth surface and the like.

The invention relates to the technical field of artificial dermis, in particular to a preparation method and application of artificial dermis based on bionic acellular matrix. The artificial dermis comprises a bionic acellular matrix component A and an auxiliary material component B, wherein the component A comprises type I collagen, type VI collagen, elastin, fibronectin, laminin, mother cell protein and glucan; the component B comprises antibacterial peptide, antibiotics and a high-molecular polymer material; the mass ratio of the component A to the component B is (4-6): (1-4). The preparation method comprises the following steps: respectively preparing procedure mother liquor of the component A and the component B, preparing hydrogel with a double-layer gradient structure through step-by-step crosslinking, and then carrying out freeze drying and sterilization treatment. The artificial dermis disclosed by the invention has good biocompatibility and antibacterial property, can effectively promote wound healing, is suitable for repairing acute and chronic wounds of four limbs, faces and trunk parts, and is particularly suitable for repairing complex wounds with exposed bones, tendons, nerves and blood vessels.

The present application belongs to the field of biomedical materials and tissue engineering, and particularly relates to a kind of acellular matrix aerogel repair material and its preparation method and application.Further, the present application relates to the comprehensive preparation method of the material, especially the extremely low immunogenicity acellular process combined with physical, chemical and supercritical fluid technologies, and the unique aerogel forming technology based on supercritical drying.Further, the present application relates to the application of the material, mainly related to soft tissue repair, medical cosmetology, bone / cartilage injury repair field.

The application relates to the technical field of biomaterials, in particular to a crosslinked amniotic membrane acellular matrix filling material and a preparation method thereof. The crosslinked amniotic membrane acellular matrix filling material is prepared by using a photo-crosslinking technology, and the specific preparation method is as follows: placing an acellular amniotic membrane matrix in a pretreatment agent phosphate buffer solution to perform pretreatment, then performing functionalization treatment on the acellular amniotic membrane extracellular matrix, preparing a photo-crosslinking solution, immersing the treated acellular amniotic membrane extracellular matrix in the photo-crosslinking solution, permeating at room temperature, and finally performing irradiation under a UV light source to form a stable three-dimensional network structure. The material has high mechanical strength, controllable degradability and excellent biocompatibility, and is suitable for tissue repair and regenerative medicine.

The application relates to the technical field of tissue repair, in particular to an amniotic membrane acellular matrix for filling material and a preparation method. The amniotic membrane acellular matrix with a tissue repair function is prepared from amniotic membrane tissue, and the preparation method of the amniotic membrane acellular matrix adopts specific processes such as enzymolysis, so that the content of growth factors, collagen, glycosaminoglycan and other components in the amniotic membrane acellular matrix can be effectively improved, different types of tissue repair functions can be met, the amniotic membrane acellular matrix is suitable for being used as a personalized biological filling material, and has a wide application prospect.

The invention relates to the technical field of biological materials, in particular to a preparation method and application of a nucleus pulposus acellular matrix hydrogel material. The method comprises the following steps: taking a proper amount of lumbar nucleus pulposus tissues of pigs in a slaughter house, cleaning the lumbar nucleus pulposus tissues with PBS for several times, then taking part of the lumbar nucleus pulposus tissues for treatment, and subpackaging the residual nucleus pulposus tissues at-20 DEG C Preparing a decellularized liquid working solution; preparing an acellular nucleus pulposus matrix; cleaning the acellular nucleus pulposus matrix; evaluating acellular nucleus pulposus matrix genetic material residues and extracellular matrix preservation conditions; preparing a powdery nucleus pulposus acellular matrix; preparing a methacrylated acellular nucleus pulposus matrix; detecting the cross-linking performance of the methacrylated acellular nucleus pulposus matrix; through the mode, the efficient and complete nucleus pulposus acellular matrix can be obtained, and the nucleus pulposus acellular matrix hydrogel material which has good activity, proper mechanical property, quick crosslinking, injectability and strong applicability can be obtained.

The application discloses an animal small intestinal submucosa acellular matrix anti-adhesion membrane and a preparation method and application thereof, and belongs to the field of biological materials. The animal small intestinal submucosa acellular matrix anti-adhesion membrane is obtained by sterilizing one side of the animal small intestinal submucosa acellular matrix after laser treatment. The animal small intestinal submucosa acellular matrix anti-adhesion membrane has reduced thickness, improved degradation performance, can guide postoperative wound tissue regeneration, can prevent other tissues from adhering to a wound surface, and is particularly suitable for a case that the degradation rate of a sheet material needs to be further improved, and has a wide application prospect in preparation of anti-adhesion tissue repair materials.

The utility model discloses a sterilization box for an acellular matrix, and belongs to the field of sterilization boxes. The device mainly comprises a box assembly, the box assembly comprises a box body, a cavity is formed in the box body, articles can be located in the cavity, a supporting assembly is fixedly installed at the position of the cavity in the box assembly, the supporting assembly comprises a supporting table, and the supporting table can place the articles needing to be detected in a centralized mode; according to the sterilization box for the acellular matrix, by arranging the supporting table, articles needing to be sterilized can be placed in the box body in a centralized mode, and the supporting table can be placed on the multiple sets of fixing blocks, so that the supporting table can be supported and limited in the box body through the fixing blocks; and meanwhile, through cooperation between a spring and a lower pressing plate, the supporting table can be clamped when being placed on a fixing block, so that the stability of the supporting table can be guaranteed, and the situation that the supporting table easily shakes by a large margin in the box body can be prevented.

The invention belongs to the technical field of biological tissue engineering, and discloses a fat acellular extracellular matrix hydrogel preparation method, which comprises: (1) cutting cleaned fresh fat into pieces, and repeatedly freezing and thawing with a hypertonic salt solution; (2) homogenizing the frozen and thawed fat at low temperature, and collecting and precipitating; (3) re-suspending the precipitate by using PBS, oscillating in a shaking table, centrifuging at room temperature, and collecting the precipitate again; (4) carrying out degreasing and decellularization treatment on the collected precipitate by using a composite eluent; and (5) washing the precipitate with isopropanol for 24 hours, finally repeatedly washing the precipitate with distilled water, and collecting the precipitate, namely the fat acellular matrix. And (6) digesting the fat extracellular matrix with pepsin hydrochloride, and adjusting the pH value to be neutral with sodium hydroxide to obtain the fat extracellular matrix hydrogel. According to the preparation method of the fat extracellular matrix hydrogel, biological enzyme and high-speed centrifugation are not needed in the decellularization stage, the technology is simple, time consumption is short, the yield is high, the fat extracellular matrix prepared through the method is easy to digest, and the prepared hydrogel solution is uniform, good in injectability and capable of being used for production of a filling material for injection.

The invention provides an acellular matrix fiber woven scaffold as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The acellular matrix fiber woven scaffold comprises a woven structure unit, wherein the woven structure unit comprises warp yarns and a plurality of acellular matrix fiber weft yarns; the acellular matrix fiber is prepared by cutting an acellular matrix paper-based material at equal intervals; the plurality of acellular matrix fiber wefts are arranged in parallel according to a bionic trend and are interwoven and fixed through the warps, so that a bionic pattern simulating natural tissue fiber arrangement is formed in the weaving structure unit. The acellular matrix fiber scaffold is prepared by innovatively applying a weaving technology, the scaffold can improve the cell loading efficiency, is beneficial to self-repair and reconstruction of damaged tissues, provides suitable survival and growth conditions for cells, and has good application prospects in the field of biomedical engineering, especially in the aspects of tissue repair and regenerative medicine. And huge application potential is shown.

The present application relates to a composition comprising a cross-linked hyaluronic acid gel and acellular matrix microparticles wherein the gel particles have a porous structure, the median of the pore diameter is between 50 and 1000 [mu] m, and the acellular matrix microparticles are derived from animal-derived and human-derived tissues and organs, the acellular matrix particles are preferably selected from one or a mixture of skin, pericardium, peritoneum, mucous membrane, peritoneum, tendon, muscle, bone and cartilage, and the median of the particle size of the acellular matrix particles is 5-300 microns, preferably 10-200 microns, more preferably 20-100 microns and even 30-50 microns.

The invention discloses an oral squamous cell carcinoma decellularized matrix and a preparation method of dECM hydrogel, and the preparation method of the oral squamous cell carcinoma decellularized matrix comprises the following steps: soaking the tissue in a 0.1% w / v SDS solution for 28 hours, magnetically stirring at room temperature, and replacing the SDS solution every 12 hours; deionized water is used for cleaning for at least one hour to remove SDS residual liquid; soaking and eluting residual nucleic acid fragments by using 40U / mL nuclease, and magnetically stirring at 37 DEG C for 3 hours; and washing with deionized water for at least 1 hour, and storing the obtained oral squamous cell carcinoma acellular matrix at-80 DEG C for later use. The invention also discloses application of the dECM hydrogel in preparation of a three-dimensional cell culture system and in-vitro biological models with different invasion abilities.

Acellular substrates that provide continuous 3D growth of a variety of cells and / or tissues and methods of making the same are provided. The acellular substrates have a three- dimensional (3D) macrostructure defined by a continuous matrix of extracellular matrix (ECM) material associated with a first cell type of interest and a network of microporous channels and / or chambers extending throughout the continuous matrix of ECM material associated with the first cell type of interest. Methods of forming a personalized graft using a patient's own cells is also provided.

The invention discloses application of an acellular matrix material as a tissue damage or tumor targeting probe or carrier. A preparation method of the acellular matrix material comprises the following steps: pretreating fish tissues; performing decellularization treatment on the pretreated fish tissue to obtain a decellularized fish tissue material; the preparation method comprises the following steps: freeze-drying, grinding and digesting the decellularized fish tissue material, then carrying out ultrafiltration and centrifugation to intercept filtrate with the molecular weight of less than 50KDa, and freeze-drying to obtain the decellularized matrix material. The acellular matrix material disclosed by the invention has good blood compatibility and has a targeting effect on tissue injury, especially myocardial injury parts, diabetic nephropathy tissues or tumors.

The invention provides an acellular matrix for treating stress urinary incontinence as well as a preparation method and application of the acellular matrix. Splitting the soft tissue by using a cell lysis solution to remove cell debris; removing nucleic acid, degreasing and then micronizing; and adding the soft tissue particles and the soft tissue cross-linked particles into a phosphate buffer solution according to a ratio, mixing and emulsifying to obtain the acellular matrix for injection for treating stress urinary incontinence. The cell removal rate reaches 99%, the fat residue is less than 0.3%, and the injection is injected around the sphincter of the urethra (middle section or bladder neck) and / or in the urethra according to 0.5 ml / point and 3-4 points during clinical application to play roles in filling, connecting and repairing tissues, so that the damaged urethra supporting structure is repaired, the structural integrity and strength of connective tissues and the sphincter around the urethra are maintained, and the application prospect is wide. Therefore, the symptom of stress urinary incontinence is improved.

The application discloses a preparation method of umbilical cord decellularized matrix based on perfusion decellularization technology, which comprises decellularization treatment of a complete umbilical cord by using a programmed perfusion method, and cleaning, enzymatic hydrolysis, freeze-drying and other processes. The application further discloses the umbilical cord decellularized matrix prepared by the method and application thereof. The obtained umbilical cord decellularized matrix has complete structural integrity, retains not only umbilical cord Wharton's jelly but also rich low-immunogenic extracellular matrix components in umbilical cord tissues, and has wide application prospects in the development of soft tissue injury repair, medical beauty and life beauty related products.

The invention relates to an intervertebral disc fibrous ring tissue engineering repair material and application thereof, and aims to solve the problems of difficult fibrous ring repair, high recurrence rate, complicated operation and the like in the prior art. The repair material provided by the invention has excellent biocompatibility, superelasticity and shape memory effect, and can effectively promote regeneration and repair of the annulus fibrosus, significantly improve the surgical effect and reduce postoperative recurrence. The repairing material comprises one or more compounds of collagen, acellular matrix protein, gelatin polylactic acid and expanded polytetrafluoroethylene. The materials act together to support the growth of a plurality of cells such as chondrocytes, fibroblasts and mesenchymal stem cells, provide extremely high mechanical stability and biological activity, and are suitable for being used in a complex living body environment. Through specific chemical and physical treatment processes, the composite materials are assembled into the intervertebral disc fibrous ring tissue engineering repair material capable of supporting and repairing damaged intervertebral discs. The repair material and method provide a new solution for treatment of intervertebral disc degenerative diseases, and have wide application prospects and clinical values.

The invention belongs to the technical field of polymer composite materials, and particularly relates to a preparation method and application of an acellular matrix imitating polymer composite membrane with a double-layer structure. The composite membrane is composed of three layers which are a polymer supporting layer, a gel layer rich in hyaluronic acid and protein and a functional layer containing various active ingredients in sequence, the thickness of the polymer supporting layer is 100 + / -5 [mu] m, the mass ratio of hyaluronic acid to protein of the gel layer is 1: 1, the thickness of the gel layer is 200 + / -10 [mu] m (w / w), and the thickness of the functional coating is 10 + / -3 [mu] m. The tensile strength of the bionic acellular matrix polymer composite membrane is up to 50 MPa, the elongation at break is 300% or above, the bionic acellular matrix polymer composite membrane is remarkably improved compared with an existing two-layer acellular matrix polymer composite membrane, and operability and biodegradability are comparable with those of the existing two-layer acellular matrix polymer composite membrane.

The invention relates to a preparation method of an oral keratinized gingival regeneration and repair stent and a product thereof, and the preparation method comprises the following steps: sequentially treating an acellular matrix with a first modifier, a fixing agent and a second modifier to obtain an enhanced acellular matrix; mixing the carrier solution with the antibacterial natural product solution, dropwise adding an ionic cross-linking agent, and centrifuging to obtain a precipitate, namely the drug-loaded nanoparticles; and mixing the enhanced acellular matrix, the drug-loaded nanoparticles and the covalent linking agent, washing with water, and freeze-drying to obtain the drug-loaded enhanced acellular matrix. The repair stent prepared by the invention has excellent biocompatibility, long-term stable degradation, outstanding antibacterial and anti-inflammatory effects and good mechanical properties, and can promote wound healing and revascularization.

The invention relates to a composite hydrogel material based on acellular matrixes from three different sources. According to the material, a fat decellularized matrix (DAT), a small intestine submucosa decellularized matrix (SIS) and a blood vessel decellularized matrix (Adv) are mixed in proportion, and a gel system with the temperature response characteristic is formed through a biocompatible cross-linking agent. The ternary composite acellular extracellular matrix hydrogel disclosed by the invention can keep activity under various storage conditions, so that a wider possibility is provided for cold chain preservation of the product.

The utility model discloses a screening device for acellular matrix particles, and relates to the technical field of biomedical material processing. The device is based on the fluidized bed principle and comprises a fluidized bed reactor, a feeding system, a fluid conveying system and a collecting system. The top of the fluidized bed reactor is connected with a discharging system, the middle is connected with a feeding system, and the bottom is connected with a fluid conveying system. The working principle of the screening device is as follows: the acellular matrix particles are input into the fluidized bed layer, the particles suspended on the fluidized bed layer can settle quickly under the action of fluid, and the particles with smaller particle size can rise along with the fluid and enter the discharging system. The device can realize accurate screening of acellular matrix particles, remarkably reduces the particle size fluctuation range, can be used for directionally preparing particles in a specific particle size interval, and has the characteristics of high efficiency, continuous production, simplicity and convenience in operation and high applicability.

The invention relates to the technical field of acellular matrix gel filler preparation, and discloses an acellular matrix gel filler raw material grinding device and a gel filler. The acellular matrix gel filler raw material grinding device comprises a grinding tank, the inner wall of the interior of the grinding tank is further provided with a cold storage cavity used for storing liquid nitrogen, and the cold storage cavity is internally provided with a cold storage cavity used for storing liquid nitrogen; a grinding cavity and two pushing parts are arranged in the grinding tank, the pushing parts are rotationally connected to the grinding cavity, a processing cavity for raw material circulation is formed between the two pushing parts, and the pushing parts are used for grinding newly added raw materials. Raw materials are thrown upwards to impact on water absorption materials, so that separation is achieved, the raw materials and the water absorption materials do not influence each other, then only when the raw materials which are completely dehydrated and frozen become hard, the raw materials impact the cylinder and then are ejected to the annular groove, all the raw materials are qualified to-be-ground raw materials, the overall front work is shortened, and the grinding efficiency is improved.

The invention discloses a method for simultaneously detecting mannitol and trehalose in an acellular matrix. The method comprises the following steps: S1, preparing an instrument; s2, preparing a standard solution; s3, performing methodological verification on the detection method by adopting a high performance liquid chromatograph; and S4, detecting the contents of mannitol and trehalose in the acellular matrix by adopting a high performance liquid chromatograph matched with a differential refraction detector. The method realizes rapid and synchronous separation and detection of mannitol and trehalose in an acellular matrix under the same chromatographic condition. Methodology verifies that the method is high in specificity and free of blank interference; the linear relation is good; the repeatability and the accuracy both meet the detection requirements. According to the method, the detection efficiency is remarkably improved, the detection cost is reduced, and a reliable guarantee is provided for quality control and process stability evaluation of the acellular matrix product.

The invention relates to the technical field of preparation of pig-derived acellular matrix bone repair materials, in particular to a preparation method of a novel granular xenogeneic bone repair material, which comprises the following steps: S1, selecting pig bones without specific pathogens; s2, selecting limb epiphyseal cancellous bone parts of the pig; s3, cutting the pig bones selected in the step S2 into slices, strips and particles; s4, the cut pig bone particles are put into purified water to be ultrasonically stirred and cleaned, and fat in pores is removed; s5, soaking the cleaned pig bone particles in liquid nitrogen for 1-2 hours under sealing protection, and then putting the pig bone particles into purified water with the temperature of 25-37 DEG C to be repeatedly frozen and thawed; s6, sterilizing and degreasing the pig bone particles treated in the step S5; and S7, carrying out decellularization treatment on the pig bone particles treated in the step S6. Antigen components are removed through the processes of decellularization, degreasing, partial demineralization and the like, a natural porous structure is reserved, the porosity is close to that of human bones, efficient biocompatibility is achieved, and support is provided for development of clinical-grade medical materials.

The invention relates to a particle acellular matrix and a preparation method and application thereof.The particle acellular matrix comprises acellular matrix particles with gradient particle sizes and can be used for promoting skin wound healing, and the particle acellular matrix makes full contact with a complex wound surface and is degraded in a staged mode; a favorable microenvironment can be continuously provided for migration, growth, proliferation and differentiation of cells, so that wound healing is promoted.