99 results about "Acellular matrix" patented technology

The invention discloses a preparation process of acellular matrix particles, and relates to the technical field of biomedical material processing. The invention provides an acellular matrix particle preparation process based on a biological enzyme method and a fluidized bed principle. The acellular matrix particle preparation process comprises the following steps: alkali treatment, acellular dehydration, grinding and screening. The step of screening is based on the fluidized bed screening principle, the acellular matrix particles are input into a fluidized bed layer, the particles suspended on the fluidized bed layer can settle rapidly under the action of fluid, and the particles with small particle sizes can rise along with the fluid and enter a discharging system. The preparation process can realize accurate screening of acellular matrix particles, significantly reduce the particle size fluctuation range, can directionally prepare particles in a specific particle size interval, and has the characteristics of high efficiency, continuous production, simple operation and strong applicability.

The invention discloses a preparation method of an acellular matrix containing a bioactive coating, and belongs to the field of medical beauty industry, the key point of the technical scheme is that the preparation method comprises the preparation steps of S1, preparation of the acellular matrix; s2, treating the acellular matrix; s3, rehydrating the decellularized matrix, sequentially immersing the rehydrated decellularized matrix into an activating solution, a collagen solution and an activating solution, reacting, washing the collagen matrix, and freeze-drying to obtain the collagen matrix; s4, adding the anti-inflammatory microcapsules and the growth factor nano-carrier into the hyaluronic acid solution to obtain a mixed solution; s5, the collagen matrix is immersed in an activating solution, after reaction and washing, the surface of the collagen matrix is coated with the mixed solution for reaction, after curing, washing and drying are conducted, and the acellular matrix containing the bioactive coating is obtained.The covalently-connected hyaluronic acid-collagen double-layer structure is constructed on the surface of the acellular matrix, so that biocompatibility and safety are improved; meanwhile, the problems that a traditional acellular matrix is unstable in structure and poor in mechanical strength are solved.

The invention belongs to the field of biomedicine, and particularly relates to a 3D bio-printed vascularization-promoting bladder patch and a preparation method thereof. The preparation method comprises the following steps: polymerizing prepared GelMA and Laponite to prepare GelMA-Laponite hydrogel, then mixing and printing the GelMA-Laponite hydrogel and seed cells on a bladder acellular matrix by using a 3D biological printing machine to form a GelMA-Laponite stent with a three-dimensional space structure, and crosslinking the stent by using ultraviolet light to obtain the double-layer vascularized bladder patch. The construction level of the tissue-engineered bladder patch is expected to be improved, the vascularization problem of the tissue-engineered bladder graft is solved, and a good theoretical basis and technical support are laid for promoting the clinical application process of bladder tissue engineering.

The invention belongs to the technical field of medical biological materials, and particularly relates to a sodium hyaluronate gel regeneration filler for injection and a preparation method and application thereof.The sodium hyaluronate gel serves as a carrier to disperse acellular matrix particles, the sodium hyaluronate gel forms a micron-sized porous structure in the cross-linking process through a microbubbling technology, and the sodium hyaluronate gel is prepared into the sodium hyaluronate gel regeneration filler for injection and the preparation method and application of the sodium hyaluronate gel regeneration filler. A local cross-linked network is strengthened by combining a secondary cross-linking process, high elastic modulus is realized while the content of sodium hyaluronate is reduced, so that the porous structure has excellent instant filling and shaping capability and controllable degradation behavior, the porous structure can promote permeation of hyaluronidase, the degradation period of the hyaluronidase is matched with the tissue regeneration process, and the tissue regeneration effect is improved. And the acellular matrix particles can continuously induce adhesion, migration, proliferation and differentiation of endogenous cells of tissues, synergistically promote in-situ tissue regeneration, effectively reduce repeated injection requirements and reduce long-term complication risks, and have a good application prospect in soft tissue filling and repairing.

The invention belongs to the technical field of biomedical engineering and tissue engineering, and particularly relates to an acellular matrix modified bionic micro-fluidic organ chip constructed by 3D printing composite near-field direct writing as well as a preparation method and application of the bionic micro-fluidic organ chip. According to the bionic micro-fluidic organ chip prepared by the invention, the microstructure and physiological similarity of a model are remarkably improved, the problems of insufficient bionic property, single cell and the like at present are solved, and the preparation method is simple, is suitable for batch production and solves the problem of high production cost. The invention provides a new technical direction for the construction of the bionic organ model.

The invention provides a self-assembled temperature-sensitive hydrogel for neural restoration and a preparation method thereof. The hydrogel comprises the following raw materials: poloxamer, an acellular matrix and a neural factor in a mass concentration ratio of (5-10): (10-20): (1-4), preferably 5: 15: 1. The preparation method comprises the following steps: respectively preparing a poloxamer solution and an acellular matrix solution, mixing and heating the poloxamer solution and the acellular matrix solution to form a double-network three-dimensional gel structure, adding the nerve factors, stirring or ultrasonically dispersing, and heating to form the bioactive hydrogel. The hydrogel can rapidly form a stable three-dimensional structure at the body temperature, a support is provided for nerve repair, cell adhesion and proliferation are supported, and the added nerve factors are beneficial to maintaining the activity of neurons. Meanwhile, the hydrogel is in a liquid state at a low temperature, is convenient to inject, is fixed at a body temperature, is adjustable in degradation rate, is non-toxic, is suitable for local nerve injury treatment, and improves the bioavailability and safety of a nerve repair material.

The invention belongs to the technical field of 3D biological printing, and particularly relates to bladder acellular matrix hydrogel, a 3D biological printing bladder patch and a preparation method and application of the bladder acellular matrix hydrogel and the 3D biological printing bladder patch. The bladder acellular matrix hydrogel is prepared from a methacrylated bladder acellular matrix and a photoinitiator. The bladder acellular matrix is firstly subjected to methacrylation, so that the bladder acellular matrix has the photo-crosslinking gelling characteristic, the rheological property of the bladder acellular matrix hydrogel can be improved, the mechanical stability of the bladder acellular matrix hydrogel can be enhanced, and meanwhile, the bladder acellular matrix hydrogel has the environmental characteristic of simulating a bladder tissue extracellular matrix and does not cause immune response. Bio-ink prepared from the bladder acellular matrix hydrogel has the characteristics of shear thinning and temperature response capability, and bladder tissue engineering patches can be printed by using a 3D bio-printer.

The invention relates to an aquatic animal-derived acellular matrix hydrogel, which comprises an acellular matrix and a non-biological auxiliary agent, and a preparation method of the aquatic animal-derived acellular matrix hydrogel. The invention further relates to application of the aquatic animal-derived acellular matrix hydrogel in preparation of a product for wound repair or application of the aquatic animal-derived acellular matrix hydrogel in preparation of a product for surgical operation.

The invention provides an in-situ tissue engineering valve stent material as well as a preparation method and application thereof, and belongs to the technical field of medical materials. According to the invention, the surface of the acellular matrix is loaded with the super-particle coating formed by self-assembly of the polyphenol-mediated polypeptide aggregate and the Prussian blue nano-enzyme, so that the stent material is endowed with double ROS regulation and endothelial cell adhesion promoting functions, excessive deposition of collagenous fibers can be prevented, and fibrosis of valve tissues is avoided; the synergistic effect of the components of the super-particle coating promotes the endothelialization process of the stent and the remodeling of a functional extracellular matrix, so that the accelerated formation of the functional endodermis is finally realized, and a powerful guarantee is provided for the success of clinical transformation of an in-situ heart valve tissue engineering technology.

The invention discloses an implantable acellular matrix material and a preparation method thereof, and belongs to the field of biomedical materials. According to the method, natural animal tissues (such as pericardium, achilles tendon or small intestine submucosa) are used as raw materials, a low-temperature chemical combined decellularization treatment technology is adopted, and the method comprises the multi-step synergistic process of pre-cooling soaking balancing, low-temperature enzymolysis, low-concentration surfactant gradient permeation cleaning, low-temperature nuclease degradation, non-crosslinking sterilization and the like. Under the condition that the treatment temperature is strictly kept at 0-8 DEG C, cell components and genetic materials (the residual DNA content is 1t, and the dry weight is 8 ng / mg) are efficiently removed, and meanwhile the natural three-dimensional fine structure of an extracellular matrix (ECM), the integrity of collagen fibrils and key bioactive components (such as glycosaminoglycans (GAGs)) are reserved to the maximum extent. The host immunological rejection reaction of the obtained material is remarkably reduced, host cell ingrowth, vascularization and tissue function reconstruction can be effectively promoted after implantation, and the material is suitable for high-end implantation scenes such as soft tissue repair and regenerative medical stents.

The invention discloses a preparation method of a 3D printing acellular matrix skin scaffold for slowly releasing a mechanical stimulation exosome. The preparation method comprises the following steps: (1) preparing acellular dermal matrix powder; (2) mixing acellular dermal matrix powder with methacrylated chitosan, the mass of the acellular dermal matrix powder being 10% of that of the methacrylated chitosan, and then mixing the acellular dermal matrix powder with the fibroblast exosome prepared in the claim 1 to obtain bio-ink, the concentration of the exosome being 150 [mu] g / ml; and (3) printing the bio-ink obtained in the step (2) into a skin stent by adopting a 3D bio-printer, and soaking the stent in a 1% tannic acid solution for 15 minutes to finish surface modification, wherein a printing model is a 2cm * 2cm * 2mm rectangular stent. The acellular matrix skin scaffold provided by the invention provides a good slow-release carrier for the fibroblast exosome subjected to mechanical stimulation, and the slow release and long-term play of the exosome can be realized through the scaffold.

The invention discloses polycaprolactone / acellular extracellular matrix composite hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological tissue engineering. The amino-modified polycaprolactone nanofiber and the fat acellular matrix are compounded in proportion for the first time, genipin is used as a cross-linking agent of the composite hydrogel, and the hydrogel of an injectable composite material is prepared. The crosslinking degree, the gelation time, the degradation performance, the swelling performance and the mechanical performance of the composite hydrogel with different proportions are studied, and the influence of the composite hydrogel on angiogenesis and fat regeneration in vivo is studied. According to the novel injectable composite hydrogel disclosed by the invention, the 1% PCL / DAT group composite hydrogel has good adipogenesis and vascularization effects, and a new thought is provided for subsequent soft tissue repair and regeneration research.

The invention relates to the technical field of tissue repair, in particular to an amnion decellularized matrix for a filling material and a preparation method of the amnion decellularized matrix. The amnion decellularized matrix with the tissue repair function is separated and prepared from amnion tissue, specific processes such as enzymolysis are adopted in the preparation method of the amnion decellularized matrix, and the content of growth factors, collagen, glycosaminoglycans and other components in the amnion decellularized matrix can be effectively increased; the material can meet the repairing function of different types of tissues, is suitable for being used as a personalized biological filling material, and has a wide application prospect.

An artificial meniscus and a preparation method therefor. The preparation method comprises: uniformly punching an acellular matrix material, and filling the pores with collagen, wherein the acellular matrix material is the skin tissue of pigs, cattle, etc., has a high biomechanical performance, and is filled with collagen after punching, which is beneficial for cell adhesion, proliferation and migration, and can induce meniscus regeneration. In addition, the acellular matrix material and the filled collagen are subjected to a cross-linking treatment, thereby having a controllable biodegradability and being adaptable to the rate of meniscus tissue formation. The artificial meniscus prepared in the present invention has a good biocompatibility, biomechanical performance and biodegradability.

According to the preparation method of the moisturizing wound protecting membrane, the wound protecting membrane is of a double-layer structure and comprises a polyurethane outer membrane and a composite membrane composed of collagen sponge and an animal intestine membrane, and the preparation method of the moisturizing wound protecting membrane comprises the following steps that S1, the polyurethane outer membrane is prepared; s2, preparing a film layer; s3, preparing a collagen layer; s4, freeze-drying and molding the collagen layer and the membrane layer in a mold to obtain a collagen membrane; and S5, bonding the obtained polyurethane outer membrane with a collagen membrane to prepare the double-layer wound protecting membrane. The wound protecting membrane provided by the invention adopts a design of tightly combining three layers, the acellular matrix middle layer can be gradually degraded to inhibit scar hyperplasia, the polyurethane outer membrane provides a physical barrier and promotes cell adhesion and wound healing, and the three-dimensional porous collagen sponge bracket accelerates vascularization and cell ingrowth, so that the wound protecting membrane has a good wound healing effect. A novel decellularization technology is integrally adopted to retain biological activity, and an environment beneficial to skin growth is constructed through a double-layer structure.

An acellular matrix woven material, a preparation method and an application thereof are provided. The acellular matrix woven material is prepared by combining 3D printing with weaving. At the same time, also provided are the acellular matrix woven material prepared by the preparation method and its application in preparing tissue engineering scaffold materials.

The present disclosure relates to novel pharmaceutical compositions and their use in general tissue reconstructive and augmentative methods. The pharmaceutical compositions comprise multilayered acellular matrices, such as dermal matrices (ADMs), of two or more layers. The compositions may be used in any general tissue reconstructive or augmentative medical procedure where the goal is to increase the volume in the area of the body in which the multilayered ADM compositions are introduced.

The invention relates to a nasal administration preparation containing extracellular vesicles and acellular matrix hydrogel and a preparation method of the nasal administration preparation, and belongs to the technical field of extracellular vesicles. According to the nasal administration preparation, the nasal administration preparation contains extracellular vesicles, acellular matrix hydrogel and auxiliary materials, and the auxiliary materials contain at least one of a lipophilic solvent, a humectant, a thickening agent and an osmotic pressure regulator. The EVs nasal administration dosage form is prepared, the EVs has the capacity of passing through a blood brain barrier, can be delivered to a brain central nervous system and has high brain administration capacity, the human placenta source ECM forms nanofibers through structural regulation and control, the nanofibers are used as a carrier material of the EVs, long-term storage of the biological activity of the EVs can be achieved, the local treatment time of the EVs is prolonged, and the EVs nasal administration dosage form can be applied to the brain. EVs can be efficiently absorbed in the nasal cavity, and compared with similar products, the effective component acting time is longer.

The invention provides acellular matrix hydrogel loaded with animal acellular omentum particles as well as a preparation method and application of the acellular matrix hydrogel loaded with the animal acellular omentum particles, and relates to the technical field of tissue engineering and regenerative medicine. Comprising a decellularization treatment agent, a decellularization matrix, functional particles, an enzymolysis agent, a cross-linking stabilizer, a functional additive and a solvent, the functional additive is prepared from PDA coated Fe3O4 magnetic nano particles, phosphorylated polyglycerol sebacate and liposome coated silver nano particles. The PDA-Fe3O4 magnetic nanoparticles of the functional additive generate mechanical signals under the action of an external magnetic field, a Piezo1 mechanical gating cation channel is activated to promote calcium ion internal flow, and the autonomous mineralization ability of phosphorylated polyglycerol sebacate is combined, so that the activity of the bone differentiation marker alkaline phosphatase is effectively improved, and the bone differentiation marker alkaline phosphatase is prepared. The combination of the PDA coated Fe3O4 magnetic nanoparticles and the phosphorylated polyglycerol sebacate integrates a magnetic response mineralization system.

The invention discloses an antibacterial composite periosteum for an oral cavity and a processing technology of the antibacterial composite periosteum, and relates to the technical field of periosteum. The method specifically comprises the following steps: step 1, treating defatted pig peritoneum with a decellularized solution, cleaning and sterilizing to obtain a pig peritoneum decellularized matrix; 2, treating the pig peritoneal decellularized matrix with a glyoxal solution, cleaning, and sterilizing to obtain a pretreated pig peritoneal decellularized matrix; step 3, uniformly coating the surface of the pretreated porcine peritoneal acellular matrix with an antibacterial solution, and performing sterilization, standing and freeze-drying to obtain the antibacterial composite periosteum. Wherein the antibacterial solution is prepared from the following raw material components in concentration: 1 to 2 weight percent of modified chitosan, 0.2 to 0.4 weight percent of amino-terminated polyethylene glycol and 0.01 to 0.05 weight percent of p-toluenesulfonic acid, and a solvent is a PBS (Phosphate Buffer Solution). The antibacterial composite periosteum is good in biocompatibility and has excellent clinical application value in oral surgery.

A microneedle loaded with at least curcumin, exosomes, and an acellular matrix. Curcumin is incorporated into the exosomes to form functionalized exosomes (Cur@EV), which serve as the primary active ingredient for the repair of hypertrophic scars. Cur@EV has been shown to exhibit a high affinity for hypertrophic scar fibroblasts, while more efficient delivery of curcumin to HSFs has demonstrated enhanced anti-HSF cell proliferation activity and significant anti-fibrosis effects, potentially meeting clinical needs for the repair of hypertrophic scars.

The application discloses a kind of tissue regeneration fillers and preparation method and application thereof, belong to the field of biomedical materials and medical cosmetology technical field. Including the following mass fraction components: mixed microparticle 1.0~5.0 parts;Mixed microparticle includes acellular matrix microparticle and basement membrane microparticle, and the mass ratio of acellular matrix microparticle and basement membrane microparticle is 10‑1:1.The application extracts and compounding two kinds of microparticles respectively, combines gel matrix and dispersion liquid processing, retains the scaffold function of acellular matrix, supplements basement membrane specific active ingredient, realizes instant support and long-acting regeneration synergy;Filler has excellent injectability and biocompatibility, can accurately repair basement membrane structure degradation, promote cell directional migration and tissue functional regeneration, and is widely used in facial rejuvenation, wound repair and soft tissue regeneration.

The invention discloses a composite artificial cavity / membrane tissue and a preparation method thereof. The composite artificial cavity / membrane tissue is formed by compounding a synthetic high-molecular material and a natural high-molecular biological material, and the synthetic high-molecular material is selected from more than one of silicone, synthetic high-molecular hydrogel and an acrylic polymer. The natural polymer biological material is selected from more than one of an acellular matrix scaffold material, a non-acellular matrix and non-acellular collagen. The composite artificial cavity / membrane tissue provided by the invention has the advantages of good healing after transplantation, high biomechanical strength, low immunogenicity, smooth surface and the like.

The invention relates to the technical field of artificial dermis, in particular to a preparation method and application of artificial dermis based on bionic acellular matrix. The artificial dermis comprises a bionic acellular matrix component A and an auxiliary material component B, wherein the component A comprises type I collagen, type VI collagen, elastin, fibronectin, laminin, mother cell protein and glucan; the component B comprises antibacterial peptide, antibiotics and a high-molecular polymer material; the mass ratio of the component A to the component B is (4-6): (1-4). The preparation method comprises the following steps: respectively preparing procedure mother liquor of the component A and the component B, preparing hydrogel with a double-layer gradient structure through step-by-step crosslinking, and then carrying out freeze drying and sterilization treatment. The artificial dermis disclosed by the invention has good biocompatibility and antibacterial property, can effectively promote wound healing, is suitable for repairing acute and chronic wounds of four limbs, faces and trunk parts, and is particularly suitable for repairing complex wounds with exposed bones, tendons, nerves and blood vessels.

The application provides a kind of two-component multifunctional nanometer membrane and its preparation method and application, it is related to biological medical material technical field, the nanometer membrane, by including the following mass fraction of component is prepared: high molecular matrix component one 50~75%, high molecular matrix component two 24~49%, functional component 1%;The high molecular matrix component one is hyaluronic acid or hyaluronic acid derivative, the high molecular matrix component two is one of collagen, gelatin, methacrylated gelatin or acellular matrix, the functional component includes drug and active factor;The drug is loaded on high molecular matrix component one, and the active factor is loaded on high molecular matrix component two;The nanometer membrane is formed by chemical crosslinking.This application overcomes the shortcomings and deficiencies of existing corneal alkali burn and infection treatment means, provides a kind of two-component multifunctional nanometer membrane with good biological safety, effective control infection and inhibits inflammatory response, for treating corneal injury.

The present invention provides a decellularized matrix microparticle freeze-dried implant, a preparation method and an application thereof, belonging to the technical field of soft tissue filling materials. The decellularized matrix microparticle freeze-dried implant is composed of a decellularized matrix, a suspending agent and a freeze-drying protectant, wherein the mass ratio of the decellularized matrix, the suspending agent and the freeze-drying protectant is 0.5-10:0.5-10:0.5-6; the particle size of the cell matrix freeze-dried implant is 10 μm-1000 μm. The decellularized matrix microparticle freeze-dried implant of the present invention retains the natural three-dimensional structure and natural active ingredients, reduces the immune rejection reaction caused by the raw material source after the injection product is clinically used, and is suitable for injection into irregular defects for filling and repair, injection into the deep subcutaneous dermis to the superficial subcutaneous layer to repair moderate to severe wrinkles or folds, and has a good repair effect.

The invention relates to the technical field of medical biological materials, in particular to a modified acellular matrix. According to the method, microcrystal drying, immobilization and swelling treatment are combined in sequence in the decellularization treatment process, so that the phenomenon of low porosity after crosslinking can be effectively reduced, meanwhile, the mechanical loss in the decellularization process is reduced, and the high mechanical property is considered while the high porosity is obtained. According to the modified acellular matrix, the defect that the mechanical property of an acellular matrix material with the unit thickness is insufficient is remarkably overcome, and the modified acellular matrix has high porosity and high mechanical property at the same time. The acellular matrix is thin and tough, the comfort degree is high after implantation, the tensile strength is improved to more than three times that of an original material, and the acellular matrix has extremely high application value in the medical field with requirements for mechanical property, porosity, thickness and comfort degree.

The present application belongs to the field of biomedical materials and tissue engineering, and particularly relates to a kind of acellular matrix aerogel repair material and its preparation method and application.Further, the present application relates to the comprehensive preparation method of the material, especially the extremely low immunogenicity acellular process combined with physical, chemical and supercritical fluid technologies, and the unique aerogel forming technology based on supercritical drying.Further, the present application relates to the application of the material, mainly related to soft tissue repair, medical cosmetology, bone / cartilage injury repair field.

The utility model relates to the technical field of biomedical material processing equipment, and discloses an auxiliary shaping device for an acellular matrix membrane material, which comprises a shaping mold, and the shaping mold mainly comprises a shaping template and a base; the shaping template is provided with an upper cavity with a downward opening, the top surface of the shaping template is provided with an inward concave shaping cavity, and the top surface of the shaping template is provided with a plurality of adsorption through holes; an upper cavity is formed in the upper portion of the base, a lower cavity corresponding to the upper cavity is formed in the upper portion of the base, the shaping template is detachably connected with the base in a sealed mode, the upper cavity and the lower cavity jointly form a negative pressure cavity, and an air hole connector communicated with the negative pressure cavity is arranged on the side face of the shaping template or the side face of the base and used for being connected with a negative pressure source. According to the utility model, the shaping template with the shaping cavity is designed, and the diaphragm is shaped in a negative pressure adsorption manner, so that the acellular matrix diaphragm can be prefabricated into a shape matched with various wound surfaces, the fitting degree of the diaphragm and the wound surfaces is greatly improved, and the problem that the traditional plane diaphragm is difficult to fit is effectively solved.

The application relates to the technical field of biomaterials, in particular to a crosslinked amniotic membrane acellular matrix filling material and a preparation method thereof. The crosslinked amniotic membrane acellular matrix filling material is prepared by using a photo-crosslinking technology, and the specific preparation method is as follows: placing an acellular amniotic membrane matrix in a pretreatment agent phosphate buffer solution to perform pretreatment, then performing functionalization treatment on the acellular amniotic membrane extracellular matrix, preparing a photo-crosslinking solution, immersing the treated acellular amniotic membrane extracellular matrix in the photo-crosslinking solution, permeating at room temperature, and finally performing irradiation under a UV light source to form a stable three-dimensional network structure. The material has high mechanical strength, controllable degradability and excellent biocompatibility, and is suitable for tissue repair and regenerative medicine.