248 results about "Acellular matrix" patented technology

Acellular matrix implants for implantation into an articular cartilage or osteochondral lesion and a method for fabrication thereof. A protective biodegradable polymer barrier having extended polymerization time and a method for preparation thereof. Bone-inducing compositions. A method for treatment of articular cartilage or osteochondral injuries.

The invention relates to the field of bio-materials, and especially relates to an acellular matrix repairing gel and a new method for preparing the same. The invention discloses the acellular matrix repairing gel and the method for preparing the same, wherein the method includes steps of acellular treatment and gelatinization treatment on tissue and organs from mammal animals to prepare the acellular matrix repairing gel. The acellular matrix repairing gel is eliminated in immunogenicity of heterologous and foreign tissue, so that activity of extracellular matrix components of the tissue is maintained as more as possible. The gel can specially repair damaged tissue and organs of human body, is strong in applicability, is suitable for requirements of various irregular-shaped repair zones and different position environments in body and has a huge clinical value.

An acellular matrix implant for treatment of defects and injuries of articular cartilage, bone or osteochondral bone and a method for treatment of injured, damaged, diseased or aged articular cartilage or bone, using the acellular matrix implant implanted into a joint cartilage lesion in situ and a bone-inducing composition implanted into an osteochondral or bone defect. A method for repair and restoration of the injured, damaged, diseased or aged cartilage or bone into its full functionality by implanting the acellular matrix implant between two layers of biologically acceptable sealants and / or the bone-inducing composition into the osteochondral bone or skeletal bone defect. A method for fabrication of the acellular matrix implant of the invention. A method for preparation of bone-inducing composition.

An acellular soft tissue-derived matrix includes a collagenous tissue that has been delipidated and decellularized. Adipose tissue is among the soft tissues suitable for manufacturing an acellular soft tissue-derived matrix. Exogenous tissuegenic cells and other biologically-active factors may be added to the acellular matrix. The acellular matrix may be provided as particles, a slurry, a paste, a gel, or in some other form. The acellular matrix may be provided as a three-dimensional scaffold that has been reconstituted from particles of the three-dimensional tissue. The three-dimensional scaffold may have the shape of an anatomical feature and serve as a template for tissue repair or replacement. A method of making an acellular soft tissue-derived matrix includes steps of removing lipid from the soft tissue by solvent extraction and chemical decellularization of the soft tissue.

An implant for the trapezium of the thumb carpometacarpal (CMC) joint comprising an integral elastomeric member configured to include a body portion having a tapered neck, having extending from one end thereof an elongated, longitudinally extending tapered portion adapted to be embedded into a reamed out-channel in the thumb metacarpal bone. After implantation, in the preferred embodiment of the method according to the invention, a segment of a nearby tendon, for example, the APL or FCR tendons, may be wrapped around the tapered neck of the implant to secure it in position, thereby forming a reinforced structure to inhibit dislocation of the prosthesis. However, in other methods, various other securing elements, including, but not limited to acellular matrices, may be used to retain the implant in position.

The invention discloses a preparation method of acellular matrix gels, which is implemented through that natural tissues or organs are treated by using a decellation technology, the obtained object is subjected to subsequent chalking and enzymatic digestion treatment so as to obtain an acellular matrix solution, and the acellular matrix solution can be prepared into acellular matrix gels with various shapes and properties under mild conditions. Acellular matrix gels prepared by using the preparation method disclosed by the invention can be used in injectable gels and can be processed and formed into gels, the formed gel has a nano fiber microstructure, and the microstructure has a positive effect on regulating the behaviors of cells.

The invention discloses an acellular matrix with natural level of glycosaminoglycan and preparation and application thereof. The preparation method comprises the following steps: carrying out acellular disposal on natural biological tissue to obtain an acellular matrix; detecting missing amount of glycosaminoglycan in the acellular matrix; and supplementing glycosaminoglycan by means of natural ingredients in the acellular matrix to obtain the acellular matrix with natural level of glycosaminoglycan. Through the method, a lot of the glycosaminoglycan component in the acellular matrix is recovered. The acellular matrix has good biocompatibility. The natural biological structure is recovered, and the acellular matrix is beneficial to cell growth. glycosaminoglycan is supplemented for the acellular matrix, and a tissue engineering carrier is constructed. According to the invention, a biological carrier which meets cell growth requirement and mechanical strength requirement and has similar structural components as a natural extracellular matrix can be prepared rapidly. The invention is a great breakthrough of the tissue engineering construction technology. Meanwhile, principle of the invention is reliable, the technology is simple and flexible, product reproducibility is good, and industrialization is easy to realize.

The invention discloses a tissue engineering bone / cartilage double-layer scaffold and a construction method and application in medical science. The method adopts natural cartilage and bone as materials, respectively removes the antigenicity of the cartilage and the bone through de-cell, and adopts a freezing and drying method to prepare the double-layer scaffold simultaneously suitable for growthof the two issues of the bone and the cartilage. A preparation method comprises that: acellular bone is soaked in suspension of cartilage acellular matrix microfilaments, and the double-layer scaffoldof which the upper layer is a cartilage acellular matrix three-dimensional porous spongy scaffold and the lower layer is an acellular bone scaffold is prepared by the freezing and drying method and acrosslinking method. The cartilage acellular matrix spongy scaffold part of the double-layer scaffold can be introduced with factors promoting the formation of the cartilage, and the acellular bone part can be introduced with factors promoting the formation of the bone, and the factors can be respectively inoculated with cells for forming the cartilage or the bone to construct a living tissue engineering bone / cartilage complex, which is used for repairing bone and cartilage complex defect or total joint defect clinically.

An acellular matrix implant for treatment of defects and injuries of articular cartilage, bone or osteochondral bone and a method for treatment of injured, damaged, diseased or aged articular cartilage or bone, using the acellular matrix implant implanted into a joint cartilage lesion in situ and a bone-inducing composition implanted into an osteochondral or bone defect. A method for repair and restoration of the injured, damaged, diseased or aged cartilage or bone into its full functionality by implanting the acellular matrix implant between two layers of biologically acceptable sealants and / or the bone-inducing composition into the osteochondral bone or skeletal bone defect. A method for fabrication of the acellular matrix implant of the invention. A method for preparation of bone-inducing composition.

The invention discloses a method of preparing acellular matrixes by using phospholipase. The method of the invention is characterized in that stand-by organ tissue is first pre-treated and then added into solution containing the phospholipase to prepare the acellular matrixes under a controlled condition; the prepared acellular matrixes are then washed. By adopting the preparation method of the invention, the obtained acellular matrix can have good physical property and biological function. Therefore, the preparation method in the invention is not only a great breakthrough in the tissue engineering, but also opens a new way for clinical treatment of diseases. The preparation method of the invention has the advantages of reliable theory, simple and flexible process technique, good product reproducibility and is very easy to be industrialized.

The invention aims at providing a novel full-thickness biological cornea used for transplantation. The full-thickness biological cornea takes an animal cornea acellular matrix as a carrier, and the acellular matrix comprises animal cornea matrix cells, epithelial cells and endothelial cells which are cultured and augmented in vitro. The cornea is characterized in that the xenogenic corneal acellular matrix prepared by a biochemical method can be used as a good carrier for in vitro constructing the biological cornea in the aspects of shape, structure and biological compatibility, the matrix cells, epithelial cells and endothelial cells of the cornea are planted respectively, and dynamically cultured in the simulated in vivo environment of a biological reactor, so that the full-thickness biological cornea with nearly normal tissue structure and characteristics can be constructed in vitro, and the biological cornea can be used to simulate the physiological cornea for fundamental research on physiology, pathology and pharmacology; moreover, the biological cornea can also be directly used as the donator for corneal transplantation.

There is provided scaffolds for tissue repair / augmentation / implant comprising an acellular matrix, a biocompatible polymer and a biomimetic agent. The scaffolds advantageously supports cell growth in the target tissue. There is also provided a method for the preparation of the scaffold and for monitoring the functionality of the scaffold in tissue using dynamic contrast-enhanced magnetic resonance imaging.

The invention relates to a biological material for repairing meniscus tear and a preparation method for the biological material. The material consists of an acellular matrix membrane on the inner layer, a porous structure collagen matrix membrane on the middle layer, a cell layer formed by cartilage cells on the outer layer and a cell sheet. The repair material has certain elasticity and toughness and is high in biological activity; the cartilage cells can secrete cell factors per se, and nutritional ingredients can be directly obtained through articular cavity synovia, and thus, the establishment of communication link between tissue cells at both ends of the tear during the meniscus tear process is accelerated; the immigration and the infusion of the cells in the material and autologous tissue cells are promoted; the tissue regeneration and functional reconstruction time is shortened; and the ideal novel material and the preparation method for the material are provided for clinically repairing the damage of the meniscus tear (including blood supply zones), especially the tissue regeneration and the functional reconstruction of no-blood zones and low-blood zones.

An acellular matrix implant for treatment of defects and injuries of articular cartilage, bone or osteochondral bone and a method for treatment of injured, damaged, diseased or aged articular cartilage or bone, using the acellular matrix implant implanted into a joint cartilage lesion in situ and a bone-inducing composition implanted into an osteochondral or bone defect. A method for repair and restoration of the injured, damaged, diseased or aged cartilage or bone into its full functionality by implanting the acellular matrix implant between two layers of biologically acceptable sealants and / or the bone-inducing composition into the osteochondral bone or skeletal bone defect. A method for fabrication of the acellular matrix implant of the invention. A method for preparation of bone-inducing composition.

The present invention relates to a support device for breast prosthesis based on an acellular matrix material of a biological tissue. The support device for breast prosthesis is formed by tailoring a membranaceous material into a petal-shaped planar biological matrix blank and by connecting adjacent edges thereof. The support device can match with the breast prosthesis; and provide a support, fixation and coverage for the breast prosthesis in various surgical operations such as mammoplasty and breast orthopedics, to avoid the ptosis and translocation of the breast prosthesis, thereby achieving desirable effects of plastic surgery. Once being implanted, the matrix material of a biological tissue can be integrated into the surrounding breast tissue of the subject, and thus transformed into new tissues in human body without the retention of foreign bodies. The present invention further relates to a method for manufacturing a support device, and a membranaceous biological matrix material.

The invention belongs to the field of tissue engineering, and discloses a vascularization support for tissue engineering and a construction method of the vascularization support. The construction method includes (1), pre-treating an acellular matrix to remove impurities possibly introduced in decellularization and storage processes; (2), producing an acellular matrix / composite material compound support according to a tissue engineering support forming method; (3), post-treating the acellular matrix / composite material compound support obtained in the step (2), and removing residual substances in the production process to obtain the vascularization support for tissue engineering. The construction method has the advantages that the method is based on basic principles in organism vascularization processes and is conformity to migration and multiplication features of endothelial cells, microenvironments for organism vascularization are simulated to the greatest extent, the cellular matrix / composite material compound support is construed to develop advantages of both the acellular matrix and a composite material, and the support product capable of meeting tissue engineering vascularization requirements is constructed.

The invention relates to the technical field of biological medical engineering, and discloses a biological repairing material for an abdominal wall defect and a preparation method of the biological repairing material. The method comprises the following steps: (1) performing pre-treatment on the muscular tissue of rectus abdominis of a dead animal; (2) performing cell extraction treatment to prepare a skeletal muscle acellular matrix biological thin sheet; (3) dissolving the skeletal muscle acellular matrix biological thin sheet into pepsase for digestion; (4) performing electrostatic spinning on skeletal muscle acellular matrix microparticles obtained by digestion and polycaprolactone to obtain a polycaprolactone / skeletal muscle acellular matrix blended electrostatic spinning fiber film which is the biological repairing material disclosed by the invention. According to the method, a skeletal muscle acellular matrix from abdominal wall tissue and polycaprolactone are combined by an electrostatic spinning technology; the preparation materials can be degraded, and products from degradation are harmless to a human body; the prepared biological repairing material can well simulate constituents and the structure of an extracellular matrix and is good in biocompatibility and biomechanical property.

The invention relates to biological ink for 3D printing of artificial skin, and belongs to the technical field of tissue engineering. The biological ink comprises a component A used for constructing an epidermal layer, a component B used for constructing a corium layer and a component C used for constructing an acellular matrix scaffold, wherein the component A comprises first seed cells, first carrier hydrogel and first growth factor slow-release gel containing traditional Chinese medicine components, and the mass ratio of the first carrier hydrogel to the first growth factor slow-release gelis (60: 1)-(60: 8); and the component B comprises second seed cells, second carrier hydrogel and second growth factor slow-release gel. The traditional Chinese medicine components such as dragon's blood and ampelopsis japonica and growth factor slow-release gel are added, and seed cells are extracted from the skin of a patient. The biological ink has good biocompatibility, can effectively promotecell proliferation and regeneration and wound healing, has good mechanical and fluidic properties, and can be used in 3D printing skin and other related fields.

The invention provides a porcine arterial vacuum lyophilization acellular matrix, as well as a preparation method and application thereof. The preparation method of the porcine arterial vacuum freeze-drying acellular matrix comprises the following steps: performing vacuum lyophilization to the porcine arterial blood vessels to obtain the best pore by adopting an optimal pre-freezing velocity; rehydrating the matrix with low-concentration detergent and performing acellular treatment; and lyophilizing the matrix and storing at normal temperature. The matrix has the advantages that the porcine aorta is used as a studying object, and a lyophilized acellular blood vessel with proper porosity, mechanical performance and immunogenicity is produced by virtue of optimized preparation conditions; animal in-vivo experiments prove the practicality of three-dimensional blood vessel clinical vascular transplantation, and experimental basis is provided to further clinical application; the preparation method has extremely low cost, so that precious medical resources reach billions of RMB can be saved assuredly; and experimental basis and guidance are provided to clinical application of tissue engineering arterial blood vessels and optimizing research and development of acellular blood vessels.

An acellular matrix glue and a method of making is disclosed. Specifically, an acellular matrix glue that is useful in preparing a reinforced acellular matrix for medical applications including tissue engineering and hernia repair.

An acellular matrix implant for treatment of defects and injuries of articular cartilage, bone or osteochondral bone and a method for treatment of injured, damaged, diseased or aged articular cartilage or bone, using the acellular matrix implant implanted into a joint cartilage lesion in situ and a bone-inducing composition implanted into an osteochondral or bone defect. A method for repair and restoration of the injured, damaged, diseased or aged cartilage or bone into its full functionality by implanting the acellular matrix implant between two layers of biologically acceptable sealants and / or the bone-inducing composition into the osteochondral bone or skeletal bone defect. A method for fabrication of the acellular matrix implant of the invention. A method for preparation of bone-inducing composition.

The present invention relates to a production method for cryopreserved acellular dermal matrix and to cryopreserved acellular dermal matrix produced thereby, and more specifically it relates to a method in which a cryopreservation agent is made by adding sucrose to basic components consisting of glycerol and a basic solution and in which the resulting solution is used in the cryopreservation of skin tissue from which the cells in the epidermis and dermis have been removed, and relates to cryopreserved acellular dermal matrix produced thereby.

The invention provides a biological patch for pelvic floor, and a preparation method of the biological patch. The biological patch for the pelvic floor is prepared by compounding and punching a biodegradable natural acellular matrix material with low immunogenicity and good biocompatibility; after being implanted into a body, the material is gradually degraded; by means of technologies of compounding and punching, the absolute amount of an implant and the risk of immune are reduced while the mechanical support in the body is guaranteed; furthermore, the compliance between the implant material and soft tissues of the pelvic floor is improved; therefore, the regeneration and repair of the tissues of the pelvic floor are promoted, and the aim of treating POP is achieved by reinforcing soft tissue fascia.

The invention relates to a cartilage-bone integrated porous bionic scaffold and a preparation method thereof. The preparation method comprises the following steps: preparing cartilage acellular matrixpowder, and preparing bone tissue demineralized bone matrix powder; preparing gelatin or a collagen solution; preparing gelatin or collagen suspension containing a cartilage acellular matrix, and preparing gelatin or collagen suspension containing a demineralized bone matrix; preparing gelatin or collagen suspension containing the cartilage acellular matrix and the demineralized bone matrix; constructing a cartilage-bone integrated three-dimensional digital model through computer aided design, and forming a cartilage-bone integrated kernel grid framework through three-dimensional printing; fixing the cartilage-bone integrated grid framework in a cylindrical mould, pouring the gelatin or collagen suspension containing the demineralized bone matrix, preparing a bone phase scaffold, pouringthe gelatin or collagen suspension containing the cartilage acellular matrix and the demineralized bone matrix, preparing a junctional phase scaffold, pouring the gelatin or collagen suspension containing the cartilage acellular matrix again, preparing a cartilage phase scaffold, and preparing into the cartilage-bone integrated three-dimensional frozen scaffold.

The invention provides an acellular matrix material based on an adipose-derived stem cell sheet and a preparation method of the acellular matrix material. The acellular matrix material is an autologous adipose-derived stem cell sheet. According to the acellular matrix material, vitamin C is utilized to promote formation of the adipose-derived stem cell sheet, then the repeated freezing and thawing method and Triton*100 acellular treatment are used together to structure the acellular matrix material based on the adipose-derived stem cell sheet. The acellular matrix material is even in surface, uniform and homogeneous in texture and rich in content of extracellular matrix, forms a porous three-dimensional tissue, has better tensile property, low probability of breakage, no immunogenicity, capability of auto-transplantation, and operability of clinical application, is simple in preparation method and has a very high practical value.

The invention provides an assembling type cell-derived extracellular matrix membrane (ECM) compound bone repairing material as well as a preparation method and application thereof. The bone repairingmaterial is prepared by the following steps: completely covering a three-dimensional degradable biological scaffold with a sheet-shaped acellular matrix membrane under sterile conditions and then freezing the three-dimensional degradable biological scaffold for 24 to 48h under the condition of -20 to -80 DEG C; then carrying out freeze drying treatment to obtain the ECM membrane compound bone repairing material, wherein the sheet-shaped acellular matrix membrane is a cell-derived extracellular matrix which is generated by in-vitro culture of cells, is subjected to decellularization treatment and has certain bone induction activity, and the three-dimensional degradable biological scaffold is a biological scaffold which is prepared from natural polymers, has a three-dimensional porous microscopic structure and can be completely degraded and absorbed in human bodies. The assembling type cell-derived extracellular matrix membrane compound bone repairing material has a good bone induction capability; furthermore, the bone regeneration capability of the compound material is strengthened; a construction method is simple and feasible and the material can be stored for a long time and has awide clinical application potential.

An acellular soft tissue-derived matrix includes a collagenous tissue that has been delipidated and decellularized. Adipose tissue is among the soft tissues suitable for manufacturing an acellular soft tissue-derived matrix. Exogenous tissuegenic cells and other biologically-active factors may be added to the acellular matrix. The acellular matrix may be provided as particles, a slurry, a paste, a gel, or in some other form. The acellular matrix may be provided as a three-dimensional scaffold that has been reconstituted from particles of the three-dimensional tissue. The three-dimensional scaffold may have the shape of an anatomical feature and serve as a template for tissue repair or replacement. A method of making an acellular soft tissue-derived matrix includes steps of removing lipid from the soft tissue by solvent extraction and chemical decellularization of the soft tissue.