43 results about "Endodermis" patented technology

Transient gene expression is a powerful tool for plant genomics studies. Recently, the use of nanomaterials has drawn great interest. Delivery with mesoporous silica nanoparticles (MSNs) has many advantages. We used surface-functionalized MSNs to deliver and express foreign DNA in Arabidopsis thaliana root cells without the aid of particle bombardment. Gene expression was detected in the epidermis layer and in the more inner cortex and endodermis root tissues. This method is superior to the conventional gene-gun method to deliver DNA, which delivers the gene to the epidermis layer only. Less DNA is needed for the MSN method. Our system is the first use of nanoparticles to deliver DNA to plants with good efficiency and without external aids. MSNs, with multifunctionality and the capability of cargo delivery to plant cells as we demonstrated, provide a versatile system for biomolecule delivery, organelle targeting, and even agriculture, such as improved nutrient uptake.

The invention discloses health-care coarse-grain flour which is prepared from the following raw materials in parts by weight: (1) coarse cereals: 60-70 parts of wheat, 4-6 parts of corn, 4-6 parts of polished round-grained rice, and 4-6 parts of barley; (2) auxiliary raw materials: 0.2-1 parts of walnut, 0.1-0.8 parts of cattle blood, 0.15-0.6 parts of goose blood, 0.1-0.6 parts of yam, and 0.1-0.8 parts of endodermis of chicken gizzard; meanwhile, the auxiliary raw materials of the health-care coarse-grain flour disclosed by the invention further comprise0.1-0.8 parts of pig blood, 0.1-0.6 parts of edible fungus, 0.2-1 parts of peanuts, and 0.2-1 parts of cassia seeds; in addition, the coarse-grain flour also can comprises 8-12 parts of one of tapioca, broad bean meal, peameal, soybean meal and millet or sorghum flour, or an arbitrary mixture thereof. The invention discloses a process for preparing the health-care coarse-grain flour. Meanwhile, the invention also discloses a health-care coarse-grain dried noodle prepared from the health-care coarse-grain flour above and a preparation process thereof.

The invention relates to a three-component five-hole hollow fibrous membrane, which comprises the following components: 60-80wt% of polyvinylidene fluoride, 10-30wt% of polymethylmethacrylate and 10-30wt% of polrvinyl chloride; and the section of the membrane is provided with five holes. The membrane is divided into five layers from the outside to the inside, which are sequentially a cortical layer, a sub finger-shaped hole supporting layer, a thumb-shaped hole supporting layer, a sub finger-shaped hole supporting layer and an endodermis layer and is in a bidirectional asymmetrical structure. The preparation method comprises the steps of adding solvent and PVP for dissolution after mixing the materials in a certain proportion, oscillating Al2O3 nanoparticles and the solvent via ultrasound for 2 hours, evenly stirring the solution, standing still for 24h for deaeration, and then making the membrane through spinning with a dry-wet spinning process. The five-hole membrane has the advantages of large water flux, high rejection rate, excellent mechanical properties and the like, the preparation process is simple, the energy consumption and the operation cost are low, raw materials are obtained conveniently and are environmentally friendly, and the method is suitable for industrial production.

The invention discloses an endothelialization system for the surface of an induction degradable blood vessel stent. The system comprises a degradable magnetic material blood vessel stent, magnetic nano targeting grains and an external magnetic field; the degradable magnetic material blood vessel stent is implanted into a human body in a non-magnetic manner; the magnetic nano targeting grains can be in specificity combination so as to be used for cells for repairing endodermis and have magnetic response; and the external magnetic field is controllable in strength and is used for leading the degradable magnetic material blood vessel stent to magnetize outside the human body so as to generate a high magnetic field. In the technical scheme, after the degradable magnetic material blood vessel stent is implanted in the human body, magnetization is realized under the effect of the external magnetic field so as to generate the high magnetic field, the cells which are combined with the magnetic nano targeting grains and used for repairing the endodermis are attracted to the surface of the stent, fast endothelialization of an implanted portion of the stent is realized, targeting property of the magnetic nano targeting grains is good, application quantity is controller, and the magnetic blood vessel stent can be degraded gradually and disappears finally. The endothelialization system is applicable to patients with coronary heart disease, and simultaneously has reference significance for diseases of cancer, tumor and the like.

The invention relates to a method for preparing a single-endoderm hollow fiber pervaporation membrane, and belongs to the membrane separation field. The pervaporation membrane has very strict requirement on the integrity and the compactness of a separating layer, and the existing dry-wet phase inversion method is difficult to directly prepare the flawless hollow fiber pervaporation membrane. Because the inside diameter of a hollow fiber membrane ranges from 0.5 to 1.5mm commonly, a coating method is hard to be directly adopted to compound the separating layer to the inner surface of fiber. Therefore, the preparation of the single-endoderm hollow fiber pervaporation membrane is a technical difficult problem. In the method for preparing the single-endoderm hollow fiber pervaporation membrane, a recycle pump is utilized to pump polycation and polyanion solution to a hollow fiber inner-cavity alternately and repeatedly; a certain negative pressure is exerted outside the fiber to form a vacuum side to realize that polyion solution is filtered under the negative pressure, thereby forming a composite layer with certain separated function on the inner surface of the hollow fiber. Pervaporation experiment shows that the preparation method can be utilized to prepare the single-endoderm hollow fiber pervaporation membrane with high performance.

The invention relates to Ligustrazine acylpiperazine ramifications and their preparing method as well as their medicine combination and the application, belonging to the technical field of Ligusticum wallichi acrylpiperazine remification medicine. The invention alkylates piperazine cycle of 2-chloromethyl-3, 5, 6-trimethyl pyrazine hydrochloride with N-acylperazine to obtain Ligustrazine acylpiperazine ramifications A6-8, 16-28, 30, and 32-36, and acylates the perazine cycle of 2-chloromethyl-3, 5, 6-trimethyl pyrazine hydrochloride with 2-(1- piperazine methyl)-3, 5, 6-trimethyl pyrazine to obtain Ligustrazine acylpiperazine ramifications A1-5, 9-15, 29, 31, 37, and 38, and ammonolyzes the compound A1 with water to obtain another Ligustrazine acylpiperazine ramification A39. These ramifications and medicine auxiliaries are made into different forms of medicine combinations, used to prepare a medicine for repairing and protecting blood vessel endodermis cells or resisting blood platelet coacervation.

The invention relates to the technical field of agricultural environment conservation and restoration, in particular to a repairing agent for treating alkaline cadmium-contaminated soil and a preparation method thereof. The preparation method comprises the following steps: weighing blast furnace slag, sepiolite, bentonite and flue gas desulfurization gypsum in proportion, uniformly mixing, addingfulvic acid, and mixing to obtain the repairing agent. The preparation method of the invention has the following advantages: the raw materials are easily available; the operation is simple; the preparation cost of the repairing agent can be controlled within 800 yuan/ton, the dosage per acre is 100-300 kg, the cost of the repairing agent per acre is only 80-240 yuan, and the repairing agent can bewidely promoted and used at large scale. By the principle of multi-dimensional repairing, the activity of cadmium in soil can be reduced through sedimentation, adsorption and complexation, and the upward transfer of cadmium in crops can be inhibited through deposition on the endodermis and formation of -SH (sulfhydryl). The two-pronged approach makes it possible to effectively reduce the contentof cadmium in agricultural products.

The invention is concerned with a kind of vascular endothelial cell aneurysm bracket and its produce method, belonging to biology medical treatment material field. This method takes It adheres the amplified vascular endothelial cell of blood vessel gathered from endoderm of body vena on the surface of metal aneurysm bracket with innocuous macromolecule biomaterial coating with innocuous macromoleculem, biocompatibility and biodegradable characters. It speeds the producetion and cover of rebirth endodermis to the neck of tumour after setting up the bracket, reduces the recrudescence of aneurism and avoids the straitness of artery with tumour, enhances the cure rate to aneurism, especially to intracranial aneurysm, and reduces the thrombosis inside of artery. It overcomes the shortages of stainless steel bracket without the problem of immunogenicity and ethic. This mature technology with low cost is benefit to enhance and drive the development of new-style stuff to endovascular treatment and the interventional therapy of intracranial aneurysm.

The present invention relates to a recombinant herpes simplex virus and a method of gene treat of human tumour by using the recombinant virus, particularly relates to a recombinant herpes simplex virus with blood vessel chalone and endodermis chalone albumen, and its application in treating lung cancer. The invention also relates to medicine compound containing the recombinant virus. The recombinant herpes simplex virus of the invention can represent blood vessel chalone and endodermis chalone amalgamation albumen having effect of restraining the generation of blood vessel in the tumour cell, and the virus disintegratively increases in the tumour, such that the purpose of treating lung cancer by restraining the generation of blood vessel and disintegratively decomposing tumour cell is achieved.

This invention discloses a method for testing the composition of fatty acid of faveolus tiny regions of endodermis cell membranes of blood vessels, which cultivates blood vessel endodermis cells, breaks them to separate their faveolus regions by a discontinuous cane sugar density gradient super speed centrifugal method to extract the fatty acid of the separated faveolus tiny regions and test the composition of the fatty acid by the gas phase chromatogram method.

A new medical therapy, and more particularly certain quinoline compounds which are anti-angiogenic integrin aplha5beta1 inhibitors, for use in the treatment of fibrosis, or a fibrosis-related disease, such as a fibrotic disease affecting the lung, liver, kidney, skin, epidermis, endodermis, muscle, tendon, cartilage, heart, stomach, large intestine, small intestine, colon, uterus, nervous system, testis, adrenal gland, artery, vein, biliary tract, or eye.

The invention discloses a constructing device and a constructing method of an artificial liver lobule functional unit. The constructing device comprises a liver plate basal layer, a liver parenchyma cell zone and a fluid passageway layer, wherein a liver plate patterning groove is formed in the liver plate basal layer through processing; the fluid passageway layer comprises a fluid inlet passageway, an endothelial cell oriented growth zone and a fluid outlet passageway. The constructing method corresponding to the constructing device comprises the following steps: paving collagen containing liver parenchyma cells to the inner part of the liver plate patterning groove, bonding the liver plate basal layer with the fluid passageway layer after solidifying the collagen, pouring an endothelialcell suspension solution to the endothelial cell oriented growth zone, and enabling a cell culture medium solution to unidirectionally flow in the fluid inlet passageway, the endothelial cell orientedgrowth zone and the fluid outlet passageway after increasing endothelial cells to a certain amount. According to the constructing device and the constructing method, disclosed by the invention, reallayer-to-layer position relationship between endodermis and liver parenchyma cells is realized under dynamic culturing, the response of the liver parenchyma cells to physicochemical and biochemical irritation under the protection of the endodermis of blood vessels more tends to reality, and a more accurate result can be obtained when the artificial liver lobule functional unit is applied to drug screening, case research and therapy detection.

The invention discloses a medicine for treating coronary heart disease, which comprises the following compositions in weight portion: 15 portions of rhizoma gastrodiae, 18 portions of dogwood fruit, 12 portions of saffron, 18 portions of rhizoma polygonati, 12 portions of hemlock parsley, 20 portions of salvia miltiorrhiza, 12 portions of peach kernel, 10 portions of red paeonia, 10 portions of secretio bufonis, 50 portions of notoginseng, 24 portions of radix astragali, 18 portions of ginseng, 15 portions of aconite, 12 portions of cassia twig, 18 portions of root of straight ladybell, 12 portions of earthworm, 60 portions of fresh hawthorn, 30 portions costus root, 30 portions of endodermis of chicken gizzard, 3 portions of muskiness, and 6 portions of borneol. The invention has the advantages of no side effect, no stimulation, significant healing efficacy, effective rate of 86 percent, and curative rate over 65 percent.

The invention relates to a preparation method for lark feedstuff. The feedstuff comprises the following compositions in percentage by weight: 10 to 12 percent of pea meal, 8 to 10 percent of corn meal, 10 to 12 percent of yolk powder, 5 to 7 percent of fresh clamworm powder, 6 to 8 percent of peanut cake powder, 8 to 10 percent of worm powder, 12 to 14 percent of white fish meal, 4 to 6 percent of fishbone powder, 6 to 8 percent of apple powder, 10 to 12 percent of carrot powder, 2 to 4 percent of cod-liver oil, 0.5 to 1.5 percent of vitamin B1, 0.5 to 1.5 percent of psoraleae, 0.5 to 1.5 percent of eucommia, 0.5 to 1.5 percent of unibract fritillary bulb, 0.5 to 1.5 percent of endodermis of chicken gizzard, 0.5 to 1.5 percent of phellodendron, 0.5 to 1.5 percent of plantain seed and 0.5 to 1.5 percent of red sage root. The preparation method comprises the following steps: putting the powder agents in a stirrer according to the proportion and stirring for 130 minutes; taking the powder agents out and putting the powder agents in a pulverizer to crush the powder agents into powder; taking the powder out and putting the powder in a drier; controlling the dryness degree of the dried substances to between 70 and 85 percent and the drying time to between 40 and 60 minutes; taking the dried substances out and putting the dried substances in an expander for expansion; and controlling the temperature of the expander to between 75 and 85 DEG C, the expansion time to between 1 and 2 hours and the dryness degree of the expanded substances to between 90 and 95 percent.

The invention belongs to the field of cytobiology, and relates to a nano probe transfected adipose-derived cell and application thereof in preparing an MRI (magnatic resonance imaging) dynamic tracer. The adipose-derived progenitor/stem cell is separated and screened from adipose tissues, the phenotype of the adipose-derived progenitor/stem cell is identified by stream mode, immune fluorescence, molecular biology and other methods, and the inward epidermic cell differentiation is induced. After the nanoparticle labeled adipose-derived endodermis progenitor/stem cell is prepared into an MRI dynamic tracer, the in-vivo transplantation vascular injury restoration test, which is used for evaluating vascular endodermis conditions by transmission electron microscope, immunofluorescence, real-time PCR (polymerase chain reaction) and other methods according to the in-vivo survival, migration, outcome and restoration vascular injury conditions of the nuclear magnetic resonance dynamic tracing adipose-derived endothelium progenitor/stem cell at different time points, proves that the nano probe transfected adipose-derived cell can illuminate the mechanism of the adipose-derived endodermis progenitor/stem cell in vascular injury restoration. The nano probe transfected adipose-derived cell can provide new practice data for vascular injury treatment as well as new seed cell sources and experimental reference.

It is found that Hibiscus sabdariffa Linn extractive has the functions of reducing triglyceride and cholesterin in blood, inhibiting the oxidation of low-density lipoprotein, decreasing the fat deposition on arterial endodermis, improving the smooth muscle migration caused by spumescent cell formed on the blood vessel wall, etc. The present invention provides the application of the Hibiscus sabdariffa Linn extractive in preparing medicine for inhibiting cardiac and cerebral vascular diseases.

The invention relates to a preparation method for azure-winged magpie feedstuff. The feedstuff comprises the following compositions in percentage by weight: 10 to 12 percent of soybean protein powder, 8 to 10 percent of broomcorn protein powder, 8 to 12 percent of yolk powder, 9 to 11 percent of carrot powder, 7 to 9 percent of cornmeal, 10 to 14 percent of broomcorn powder, 5 to 7 percent of red worm powder, 6 to 8 percent of perilla frutescens powder, 7 to 9 percent of golden millet powder, 2 to 6 percent of cod-liver oil, 2 to 4 percent of vitamin B complex, 1 to 3 percent of mulberry leaf, 1 to 3 percent of endodermis of chicken gizzard, 1 to 3 percent of turtleback, 1 to 3 percent of fruit of glossy privet, 1 to 3 percent of rhizoma anemarrhenae and 1 to 3 percent of asparagus root. The preparation method comprises the following steps: putting the powder agents according to the proportion in a stirrer to stir for 130 minutes; taking out and putting the mixture in a pulverizer to crush the mixture into powder; taking out and putting the powder in a drier for drying; taking out and putting the dried powder in an annular die-type expander for expansion; taking out and putting the expanded powder in an annular die-type hard granulation machine so as to produce depositional hard granular feedstuff; sieving by a 10-mesh sieve and ensuring that the weight of each granule ranges from 0.02 to 0.03g so as to carry out sealed package according to required weight.

The present invention relates to a method for detecting fungi grown in the plant body. Said invention has two method, and its first method is applicable to seedling, and includes the following steps: soaking grass sample in NaOH aqueous solution whose concentration is 5-15% for 3-5 hrs, then making staining treatment and detection; and its second method is applicable to plant, and includes the following steps: firstly, boiling sample leaf sheath in boiling water for 4-8 min, strip-taking endodermis along inside of leaf sheath, then staining endodermis and making detection. Said invented method is applicable to detection of grass whose texture is harder, and its detection accuracy is high.

The invention provides a polylactic-acid-nanometer-particle vector which can carry therapeutic gene fragments and induct the fragments directly to cerebral vascular endothelial cells. The invention, whose transfection efficiency is higher than the current vector, has the characteristics of tissue directionality and brain tissue accessibility. The invention, with good biological compatibility, is an ideal carrying tool. The invention simultaneously discloses a method for preparing PLA nanometer used for carrying NF- Kappa B decoy oligonucleotide fragments and the application in preparing the medicine for preventing cerebral thrombosis.

The invention provides an acid preparation composition for improving the production performance of meat pigeons and a preparation method and application of the acid preparation composition. The acid preparation composition comprises a coating wall material, a binder, a Tween 80 emulsifier, silicon dioxide, an organic acid compound, an essential oil compound, nojirimycin (mulberry leaf extract), a blood replenishing compound and white carbon black. According to the invention, stomach pH can be rapidly reduced, conversion of pepsinogen into pepsase is promoted, and pepsase activity is improved, so that digestion of nutrient substances such as protein and the like is improved; on the other hand, the essential oil such as the carvacrol and the cinnamyl aldehyde has the effects of accelerating intestinal villus surface maturation and intestinal epithelial cell renewal rate, promoting digestive tract endodermis maturation, stimulating intestinal villus development, increasing digestion and absorption area and promoting secretion of various digestive enzymes in intestinal tracts, so the digestion and absorption functions of the meat pigeons are improved.

The invention discloses a method for identifying Fujian Guanxi honey pomelos by a chromatography method. According to the method, firstly, acetonitrile is used for extracting chemical ingredients in each position of outer peels, endodermis, endocarp, pulp and the like of the honey pomelos; then, a gas chromatograph, a hydrogen flame ionization detector and a liquid chromatogram-ultraviolet detector is used at the same time for detection; chemical ingredients in each position of the Fujian Guanxi honey pomelos are subjected to chromatographic qualitative analysis; specific substances of cnidium lactone, limonene, alpha-linoleic acid, palmitic acid, sitosterol, oleamide and nootkatone and the like in the Fujian Guanxi honey pomelos can be accurately detected; a honey pomelo specific substance chromatographic fingerprint chromatogram is successfully built. The method provided by the invention has the advantages that the operation is simple; the qualitative detection is accurate; the detection is fast; the method belongs to a fire-new fast, accurate and simple honey pomelo origin traceability and quality identification method.

The invention relate sot a herbal medicine for preventing and curing hydrophobia, prepared of the water of having washed rice once 85%-90%; endodermis of Ulmus pumila Root 5%-10%; endodermis of Betula luminifera Winkl Root 4%-6%. It is prepared of pure medicinal herbs, low-cost, simple to manufacture, containing no chemcial drugs, more moderate in property, and having no toxicity and side effect on human bodies; and the clinical practice shows that it has remarkable curative effect of preventing and curing hydrophobia, and its effective rate is up to 100%.