Method for detecting fatty acid components in blood vessel endothelial cell small concave area and its use

A vascular endothelium, micro-region technology, applied in the medical field

Inactive Publication Date: 2006-08-23
NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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  • Abstract
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However, there is no report on the determination method of the fatty acid composition of Caveolae

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  • Method for detecting fatty acid components in blood vessel endothelial cell small concave area and its use

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Embodiment 1

[0023] 1. Experimental materials

[0024] Human umbilical vein endothelial cells HUEVC (American Type Culture Collection, Manassas, VA), DMEM medium, IMDM medium (Iscove's Modified Dulbecco's Medium) are from Invitrogen; bovine serum albumin (component V) and protease inhibitor cocktail Purchased from Roche Corporation.

[0025] 2. Vascular Endothelial Cell Culture

[0026] Add DMEM medium to human umbilical vein endothelial cells, and add 10% heat-inactivated newborn bovine serum (purchased from Hangzhou Sijiqing Company), penicillin 100U / ml medium, streptomycin 100U / ml to the medium culture medium in a carbon dioxide incubator at 5% CO 2 Cultured at 37°C. To regulate cell fat, cells were cultured in serum-free IMDM medium containing 0.4% (w / v) bovine serum albumin (fraction V).

[0027] 3. Isolation of caveolae-rich vascular endothelial cell membrane pit microregions.

[0028] Isolation of caveolae-rich endothelial cell membrane pit microdomains was performed using disc...

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Abstract

This invention discloses a method for testing the composition of fatty acid of faveolus tiny regions of endodermis cell membranes of blood vessels, which cultivates blood vessel endodermis cells, breaks them to separate their faveolus regions by a discontinuous cane sugar density gradient super speed centrifugal method to extract the fatty acid of the separated faveolus tiny regions and test the composition of the fatty acid by the gas phase chromatogram method.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to a method for measuring the composition of fatty acids in the small concave micro-region of the membrane of blood vessel endothelial cells, and also relates to the application of the method. technical background [0002] Cell membrane caveolae (Caveolae) is a flask-shaped invaginated microdomain with a diameter of 50-100 nm on the plasma membrane, which is in the form of a vesicle (see figure 1 ). Studies have found that this structure widely exists in various types of cells, especially abundant in endothelial cells, epithelial cells, fibroblasts, smooth muscle cells, and adipocytes. Caveolae is a special cell plasma membrane structure mainly composed of lipids and proteins. Lipid components mainly include cholesterol, glycosphingolipids (glycosphingolipids, GSLS), sphingomyelin (SM). [0003] In the early stage of research, it was always believed that the function of caveolae was mainly ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N1/28G01N30/02
Inventor 李秋荣谭力黎介寿
Owner NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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