The invention provides a human vascular endothelial cell culture solution. The human vascular endothelial cell culture solution is prepared from a basic culture medium, fetal calf serum, human recombined epidermal growth factors, human recombined fibroblast growth factors, vascular endothelial growth factors, insulin-like growth factors, vitamin C, cortisol, cholera toxin, aminoethanol and ethanolamine phosphate. Compared with a traditional vascular endothelial cell culture medium, the human vascular endothelial cell culture solution has the advantages that the culture solution can be suitable for multiple kinds of vascular endothelial cells, cell proliferation is effectively promoted, the division time is effectively shortened, the characteristics of the vascular endothelial cells are maintained, the passage number of the in-vitro human vascular endothelial cells is greatly increased, and the survival time of the in-vitro human vascular endothelial cells is greatly prolonged; meanwhile, all the ingredients of the cell culture solution can be obtained in a general laboratory, preparation is convenient, a special finished product culture medium is not needed, therefore, the cost for culturing the endothelial cells is greatly reduced, and the human vascular endothelial cell culture solution is suitable for application and popularization.