A preparation method of cell-loaded microchannel hydrogel based on sucrose fiber template

Abstract

The invention relates to a preparation method of a cell-loaded microchannel hydrogel based on sucrose fiber template, which comprises the following steps: preparing sucrose fibers, coating a polymer film on the surface of the sucrose fibers, and putting the sucrose fibers with the polymer film in the middle of a polymethyl methacrylate die; preparing a mixed solution of hydrogel sol and cell culture fluid, and pouring the sol-cell mixed liquid into the polymethyl methacrylate die; changing the temperature or carrying out ultraviolet irradiation so that the hydrogel sol is crosslinked to form a hydrogel; dissolving the sucrose fibers, sucrose powder or polyvinylpyrrolidone out of the hydrogel in a phosphate buffer solution to form a cell-loaded microchannel hydrogel; and introducing endothelial cell culture fluid into microchannels of the cell-loaded microchannel hydrogel, and culturing so that the endothelial cells grow and spread to form endothelial microchannels. The cell-loaded microchannel hydrogel prepared by the method has stable microchannel structure, the endothelial cells can not easily shed, and the cell-loaded microchannel hydrogel can wrap the cells in the hydrogel crosslinking process; and the endothelial cell culture liquid is implanted after the microchannels are formed, so as to form the endothelial microchannels.

Description

technical field [0001] The invention relates to the field of biomedical engineering, in particular to a preparation method of a cell-loaded microchannel hydrogel based on a sucrose fiber template. Background technique [0002] As human longevity increases, so does the need for donor organs in the clinic. Currently, the severe shortage of donor organs means that many patients die because they cannot obtain replacement organs. Organ deficiency has become an urgent problem to be solved in the field of biomedicine, and the proposal and development of the concept of tissue engineering provides the most potential solution to this problem. [0003] During the rapid development of tissue engineering, hydrogel has received more and more attention, and it is also the most widely used biomaterial in tissue engineering in recent years. In tissue engineering, hydrogel is often used as a simulant of extracellular matrix. The composite system of hydrogel loaded with cells can be used for...

AI Technical Summary

Problems solved by technology

However, affected by the mechanical properties of the hydrogel and cell growth, the microchannels in the cell-loaded hydrogel are usually structurally unstable, and the microchannels are easy to block, crack or collapse, and the endothelial cells are easy to fall off; The endothelial cells attached to the surface of the microchannel and the macromolecular foreign matter in the microchannel may invade the interior of the hydrogel, affecting the normal growth and functional expression of cells