Preparation method of a mechanically enhanced cell-loaded microchannel hydrogel

Abstract

The invention relates to a preparation method of a mechanically-enhanced cell-loaded microchannel hydrogel, which comprises the following steps: preparing a mixed solution of hydrogel sol and cell culture fluid, and pouring the sol-cell mixed liquid into a polymethyl methacrylate die into which capillary tubes or fibers are inserted, thereby enabling primary crosslinking; drawing out the capillary tubes or fibers, introducing the hydrogel sol into microchannels of the cell-loaded hydrogel, and reinserting the capillary tubes or fibers, or sucking excess hydrogel sol out of the microchannels and carrying out ultraviolet irradiation, thereby enabling secondary crosslinking, so that the cell-loaded hydrogel with thick hydrogel layer is formed in the wall regions of the microchannels; and drawing out the capillary tubes or fibers, introducing endothelial cell culture fluid into the microchannels of the cell-loaded hydrogel, and culturing so that the endothelial cells grow and spread to form endothelial microchannels. The introduction of the thick hydrogel layer can maintain the stability of the microchannels in the hydrogel, and prevent shedding and intrusion of the endothelial cells;and the culture fluid in the microchannels can be filtered and screened.

Description

technical field [0001] The invention relates to the field of biomedical engineering, in particular to a method for preparing a mechanically enhanced cell-loaded microchannel hydrogel. Background technique [0002] Hydrogel is the most widely used biomaterial in tissue engineering in recent years. It consists of a three-dimensional cross-linked polymer network containing a large amount of water, and has a special "soft" and "wet" environment, similar to the extracellular matrix of a living body. Hydrogel-based tissue engineering technology has been successfully used to construct some relatively simple tissue structures such as skin, bladder and cartilage. However, when constructing three-dimensional complex tissues / organs with high cell concentration and vigorous cell metabolism activities, such as heart, liver, and kidney, the nutrients diffused from the surface into the tissue will be quickly exhausted by the cells. Diffusion is difficult to ensure that the cells inside t...

AI Technical Summary

Problems solved by technology

However, affected by the mechanical properties of the hydrogel and cell growth, the microchannels in the cell-loaded hydrogel are usually structurally unstable, easily blocked, cracked or collapsed, and the endothelial cells are easy to fall off; Endothelial cells on the surface of the channel and macromolecular foreign bodies in the microchannel may invade the interior of the hydrogel, affecting the normal growth and functional expression of cells