30results about How to "Help adhesion" patented technology

The invention relates to a preparation method of a mechanically-enhanced cell-loaded microchannel hydrogel, which comprises the following steps: preparing a mixed solution of hydrogel sol and cell culture fluid, and pouring the sol-cell mixed liquid into a polymethyl methacrylate die into which capillary tubes or fibers are inserted, thereby enabling primary crosslinking; drawing out the capillary tubes or fibers, introducing the hydrogel sol into microchannels of the cell-loaded hydrogel, and reinserting the capillary tubes or fibers, or sucking excess hydrogel sol out of the microchannels and carrying out ultraviolet irradiation, thereby enabling secondary crosslinking, so that the cell-loaded hydrogel with thick hydrogel layer is formed in the wall regions of the microchannels; and drawing out the capillary tubes or fibers, introducing endothelial cell culture fluid into the microchannels of the cell-loaded hydrogel, and culturing so that the endothelial cells grow and spread to form endothelial microchannels. The introduction of the thick hydrogel layer can maintain the stability of the microchannels in the hydrogel, and prevent shedding and intrusion of the endothelial cells;and the culture fluid in the microchannels can be filtered and screened.

The invention discloses a rapid preparation method for dry silver-stained samples of freshwater ciliates, belonging to the field of biotechnology. The method of the present invention comprises: (1) picking and collecting ciliates, and cleaning the surface of the ciliates; (2) adhering the clean worm liquid obtained from cleaning to the surface of a clean glass slide treated with polylysine, and removing the ciliate liquid Divide into several small drops and blow dry; (3) Add silver nitrate solution dropwise to the surface of the worm body, and clean the residue after standing; (4) Use a halogen cold light source to irradiate and develop; (5) Rinse the scum and dry it; (6) Add neutral gum dropwise to the surface of the worm body, add a cover slip and seal the slide. The method of the invention can efficiently and high-quality prepare dry silver-stained samples of freshwater ciliates, and at the same time greatly simplifies the film-making steps, and the invention does not need to use a developing solution, thereby saving raw material costs. In addition, the sample of the present invention is irradiated and developed under a cold halogen light source after being stained with silver nitrate, which is not affected by weather factors and is easier to control.