23 results about "Macrophage proliferation" patented technology

The invention discloses a giant salamander immune regulation meat peptide and application thereof. The meat peptide comprises one or more than two polypeptides selected from SEQ ID NOs: 1-5. The polypeptide can enhance cellular immune response, such as enhancing macrophage proliferation activity, improving macrophage nitric oxide secretion level, enhancing macrophage phagocytic ability, enhancing macrophage antigen presentation ability, enhancing immune organ functions, regulating T cell subpopulation, improving cellular immune factor level and enhancing immune globulin level.

The invention discloses flammulina velutipes polysaccharide nano-liposome as well as a preparation method and application thereof, and belongs to the technical field of biological functional materials. The preparation method specifically comprises the following steps: (1) sequentially performing hot water extraction, deproteinization, alcohol precipitation, dialysis and freeze-drying on flammulina velutipes raw material powder; (2) dissolving in a phosphoric acid buffer solution; (3) mixing and dissolving phospholipid, cationic lipid and a membrane regulator in an organic solvent; (4) mixing and ultrasonic treatment; and (5) carrying out reduced-pressure rotary evaporation, hydration with a phosphate buffer solution, ultrasonic treatment and extrusion. According to the flammulina velutipes polysaccharide nano-liposome and the preparation method thereof, cholesterol is replaced by phytosterol ester to prepare the flammulina velutipes polysaccharide nano-liposome, flammulina velutipes polysaccharide (FVP) is encapsulated in a water core in the liposome, the safety is higher, and the encapsulation efficiency, the storage stability and the immunocompetence of a system are improved. In-vitro experiments prove that the phytosterol ester lipidosome has the effect of promoting macrophage proliferation and nitric oxide generation, and the effect is obviously better than that of the traditional cholesterol lipidosome under the same condition.

The invention belongs to the technical field of traditional Chinese medicine extraction, and relates to a nine-steamed nine-processed rhizoma polygonati homogeneous polysaccharide and a preparation method and application thereof, in particular to a nine-steamed nine-processed rhizoma polygonati homogeneous polysaccharide and a preparation method and application thereof. The purpose of the invention is to determine one component in the nine-steamed nine-processed polygonatum sibiricum product. NPCP-A1 is extracted by a water extraction and alcohol precipitation method, deproteinized by a Sevag method, and subjected to chromatographic separation and purification by a DEAE-52 anion exchange column and a Sephacryl S-300 gel column, and the NPCP-A1 is prepared from arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid. The proliferation of RAW264.7 cells and the secretion of NO, TNF-alpha and IL-6 can be promoted. The composition is used for preparing health-care products or medicines for improving immunity. According to the acidic polysaccharide prepared by the method disclosed by the invention, the polysaccharide plays an immune regulation role by promoting macrophage proliferation and increasing secretion of cell factors such as NO, TNF-alpha, IL-6 and the like.

This invention discloses a freeze-dried preparation process of a Cordyceps and Polyporus umbellatus antitumor complex, specifically relating to the field of biomedical technology. Specifically, it uses Cordyceps powder and Polyporus umbellatus powder as raw materials, employing a simulated intestinal solution for repeated low-temperature pulsed ultrasound extraction under specific pH conditions. Following low-temperature clarification, filtration, reduced-pressure concentration, membrane fractionation, and freeze-drying protection, a freeze-dried antitumor complex powder is obtained, exhibiting both active ingredient retention, reconstitution stability, and the ability to promote macrophage proliferation and inhibit tumor cell proliferation. This invention, through the synergistic effect of intestinal simulated solution extraction, low-temperature pulsed ultrasound, membrane fractionation, and freeze-drying protection, fully releases and stably retains the active ingredients of Cordyceps and Polyporus umbellatus, improving extraction efficiency, reconstitution, and storage stability, enabling the freeze-dried powder to simultaneously promote macrophage proliferation and inhibit tumor cell proliferation.

The invention provides a postbiotic immunomodulatory preparation based on bifidobacterium longum CD11 and a preparation method of the postbiotic immunomodulatory preparation. Bifidobacterium longum CD11 is used to prepare a metagen. The immunocompetence of RAW264.7 macrophages can be promoted by thermal inactivation of bifidobacterium longum CD11 metabolites, cell walls, cell membranes and cell content metabolite components, and the cell wall component dose-dependently promotes the proliferation of the RAW264.7 macrophages and inhibits the relative expression of mRNA genes of inflammatory factors TNF-alpha and IL-1beta. In addition, a peptidoglycan (PGN) metagen from a cell wall component can remarkably inhibit relative expression of TNF-alpha and IL-1beta mRNA genes of RAW264.7 macrophages, so that inflammation is recovered to a health level (NC group level). Therefore, the bifidobacterium longum CD11 can be used as a potential immunomodulatory metabiotic strain.

The invention discloses a tuna red meat immunity enhancing peptide and application thereof in enhancing immunity, and belongs to the technical field of biology. According to the invention, polypeptides with immunocompetence are extracted from tuna red meat, and the polypeptides comprise FLQGF and LAKFPDF. Experiments prove that the polypeptide prepared by the invention has no obvious toxic effect on cells, and can improve the proliferation rate and the phagocytic rate of macrophages and the release rate of NO to different degrees. An in-vitro immunomodulatory activity result shows that the LAKFPDF and the FLQGF can activate a TLR4 dependent NF-kB / MAPK signal channel, regulate and control the immune response of macrophages and play an immunomodulatory role. Research results of the invention provide a basis for high-value development and utilization of tuna red meat, and provide a new resource for preparation of immunopotentiation products.

A method of producing macrophages includes a culture step of culturing hematopoietic progenitor cells in the presence of a TREM2 signal activator.

The invention provides a toxoplasma gondii attenuated strain RH [delta] ku80 [delta] hxgprt capable of enhancing a macrophage function. The TUB1 is TIR1-3FLAG, and the SAG1 is CAT; [delta] OMPDC:: dhfr-ts: dhfr, [delta] OMPDC:: dhfr; [delta] UP:: dh fr-ts: hxgprt ([delta] OMPDC [delta] UP). The low virulent strain delta OMPDC delta UP is obtained by knocking out OMPDC and UP genes from a TIR1 insect strain by using a CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-associated 9) technology. The strain can effectively enhance macrophage functions including phagocytic ability, proinflammatory factor secretion and the like. The insect strain construction process comprises plasmid construction, homologous template amplification and positive insect strain screening and identification. In order to systematically evaluate the immune regulation efficiency, the regulation effect of the attenuated strain on proliferation, migration, chemotaxis, active oxygen, phagocytic function and apoptosis of macrophages is quantitatively analyzed by adopting multi-dimensional analysis technologies such as a CCK-8 method, a cell scratch experiment and a Transwell migration model. Furthermore, the mRNA expression level of inflammatory cytokines after polypide infection with macrophages is determined through qPCR, and a theoretical basis and technical support are provided for clarification of an immunoregulation network of toxoplasma gondii-host interaction and development of vaccine design based on low virulent strains.

The present invention relates to a method for preparing a kelp extract exhibiting immune-enhancing activity and to the use thereof. The kelp extract according to the present invention has the effect of significantly increasing the expression of macrophage activity biomarkers COX-2 (Genome Name: PTGS2) and iNOS (Genome Name: NOS2), and immune-related cytokines TNF-α (Genome Name: TNF), IL-1β (Genome Name: IL1B), and IL-6 (Genome Name: IL6). In animal experiments, it increases natural killer (NK) cell activity, macrophage proliferation, macrophage phagocytosis, and splenocyte proliferation. Furthermore, compared to kelp extracts prepared by conventional methods, it exhibits significantly improved immune-enhancing efficacy and can be usefully utilized in pharmaceutical compositions for immune enhancement, health functional food compositions, etc.

The invention belongs to the technical field of biological medicines, and particularly relates to a method for promoting proliferation of immature bone marrow-derived macrophages and polarization of the immature bone marrow-derived macrophages to M1 through stress application culture and application of the method. The in-vitro stress application culture method of the macrophages from the immature bone marrow has the characteristics of high stability, high amplification efficiency, polarization controllability, high purity, flexibility in application, low production cost and the like; the problems that a traditional cell factor induction method is low in amplification efficiency, poor in polarization controllability, insufficient in purity and the like are solved, the purity of the obtained M1 type macrophage reaches 90% or above, stability is high, and cost is low. The method can be applied to preparation of macrophage drugs for immunoregulation, anti-fibrosis, metabolism regulation, anti-tumor CAR-M and the like, tissue regeneration biological materials, anti-inflammatory drugs, in-vitro diagnosis products and the like, dosage forms are diversified, and a standardized cell preparation scheme is provided for the related biological medicine field.

This invention belongs to the field of biotechnology, specifically relating to a highly immunomodulatory peptide derived from silkworm pupa protein, its preparation method, and its applications. The invention provides a silkworm pupa protein immunomodulatory peptide, the amino acid sequence of which is shown in SEQ ID NO.2, SEQ ID NO.3, and / or SEQ ID NO.8. This silkworm pupa protein immunomodulatory peptide is prepared by hydrolyzing silkworm pupa protein with alkaline protease, followed by purification of the enzymatic hydrolysis product through ultrafiltration and gel filtration. The most immunomodulatory peptide is screened by determining the immunomodulatory activity index, and identified by LC-MS / MS to obtain a highly immunomodulatory peptide derived from silkworm pupa protein. This immunomodulatory peptide strongly binds to TLR2 and TLR4 / MD-2 receptors through hydrogen bonds and hydrophobic interactions, activating immunity and effectively promoting macrophage proliferation and phagocytosis. This immunomodulatory peptide exhibits good immunomodulatory activity and can be used as a potential functional food ingredient.

The present application belongs to the technical field of biological medicine, and relates to a kind of Parachlorella sp. Polysaccharide and its preparation method and application. The present application obtains a kind of uniform Parachlorella sp. Polysaccharide NGP-1 by water extraction, alcohol precipitation, column chromatography separation to Parachlorella sp.;Combining high performance liquid chromatography, methylation combined gas chromatography-mass spectrometry, nuclear magnetic resonance and other methods to determine its structural characteristics. The obtained NGP-1 can promote macrophage proliferation, enhance phagocytic ability, improve active oxygen level, induce nitric oxide and cytokine production, and up-regulate the expression of corresponding mRNA, and has significant immune enhancing activity. The Parachlorella sp. Polysaccharide NGP-1 prepared by the present application can be used as a natural immune enhancer, which widens the application range of Parachlorella sp. And has broad application prospect.

The present invention discloses an active peptide from shell nacre with anti-inflammatory and skin-repairing effects. The active peptide, NYRPVLGF, can promote macrophage proliferation and effectively inhibit LPS-induced NO release in RAW264.7 macrophages, effectively regulating the secretion of inflammatory factors from macrophages and, to a certain extent, modulating inflammatory responses. Furthermore, the active peptide has a significant proliferative effect on skin fibroblasts, promoting skin wound healing and possessing promising application value.

To provide a macrophage production method capable of yielding macrophages that have a prolonged survival rate, allow for freezing preservation, and offer versatility as a tool in scientific studies.SOLUTION: A method for producing macrophages comprises a culture step for culturing hematopoietic precursor cells in the presence of a TREM2 signal activator.SELECTED DRAWING: Figure 1

The anti-inflammatory composition promotes proliferation of macrophage and suppresses inflammation caused by inflammatory cytokine and / or NO synthase, and promotes proliferation of human fibroblast or human epidermal keratinocyte and promotes synthesis of hyaluronic acid in epidermis and dermis. To provide a skin-beautifying and promoting composition which keeps the tension of the skin, strengthens the bonding force of the skin, improves the barrier function of the skin, enhances the strength and water-retaining function of the skin surface, or promotes the anti-aging of the skin, to provide a hair-growing composition which promotes the proliferation of human epidermal keratinocyte cells, improves the strength of the skin surface, and promotes hair growth, and to provide cosmetic compositions thereof.SOLUTION: The composition comprises a high-pressure treated product of a mixture of sturgeon eggs and 1, 3-butylene glycol.SELECTED DRAWING: Figure 3

The application discloses application of a large-mouth black bass stomach polypeptide in preparation of an antioxidant and / or immunoregulation product, relates to the field of bioactive peptides, and is characterized in that the large-mouth black bass stomach polypeptide is a polypeptide product obtained by alkaline protease enzymolysis of large-mouth black bass stomach, and components with molecular weights less than 3000 Da account for more than 98% of the total peptide amount. The polypeptide has significant DPPH free radical, hydroxyl free radical and ABTS cationic free radical scavenging capacity and reducing capacity, can promote macrophage proliferation, enhance phagocytosis, promote nitric oxide secretion, up-regulate immune-related cytokine expression, and play an immunoregulation role through activation of P38 and P44 MAPK signal pathways. The application realizes high-value utilization of large-mouth black bass processing by-products, has high product safety, and has a wide application prospect.

The invention belongs to the technical field of biological medicine, and relates to micrococcus sp. Polysaccharide as well as a preparation method and application thereof. The preparation method comprises the following steps: performing water extraction, alcohol precipitation and column chromatography separation on the nannochloropsis to obtain uniform nannochloropsis polysaccharide NGP-1; the structural characteristics of the compound are determined by combining high performance liquid chromatography, methylation combined with gas chromatography-mass spectrometry, nuclear magnetic resonance and other methods. The obtained NPG-1 can promote proliferation of macrophages, enhance phagocytic ability, improve the active oxygen level, induce generation of nitric oxide and cell factors and up-regulate expression of corresponding mRNA, and has remarkable immunological enhancement activity. The prepared NPG-1 can be used as a natural immunopotentiator, the application range of the NPG-1 is widened, and the NPG-1 has a wide application prospect.

The invention discloses a method for preparing ulva pertusa polysaccharide through co-fermentation of bacillus velezensis SCAU-104 and ulva pertusa powder and application of the ulva pertusa polysaccharide. Compared with the traditional hot water extracted polysaccharide (UPP), the polysaccharide (UBV) obtained by fermentation extraction has the advantages of lower molecular weight, higher sulfate group retention and more optimized structure. And carrying out macroporous resin deproteinization, ultrafiltration desalination and chromatographic purification on the UBV, and separating to obtain the uniform polysaccharide UBV-1. Researches show that UBV mainly comprises rhamnose, galactose and glucuronic acid, and is single molecular weight acidic sulfated polysaccharide. An in-vitro experiment shows that the UBV remarkably promotes RAW264.7 macrophage proliferation and NO and proinflammatory factor release, and the immunocompetence of the UBV is superior to that of UPP. The method disclosed by the invention is green and controllable, and the obtained polysaccharide is high in purity and strong in activity and can be widely applied to development of functional foods, cosmetics, skin care products and immunomodulatory products.

The invention relates to a method for inducing proliferation and / or differentiation of stem cells and / or hematopoietic stem / progenitor cells to macrophages. The method comprises the step of activating gamma-aminobutyric acid pathways of the stem cells and / or hematopoietic stem / progenitor cells. The invention further provides a culture medium for inducing the hematopoietic stem / progenitor cells to proliferate and / or differentiate into the macrophages and application of the culture medium.

The present invention provides an albumin peptide for promoting growth and development, its preparation method and applications, belonging to the field of food science and technology. The albumin peptide provided by the present invention uses denatured egg white as a substrate. First, it is pre-hydrolyzed with chymotrypsin. After the pre-hydrolysis is completed, the temperature and pH are adjusted, and then alkaline protease and chymotrypsin are added for synergistic hydrolysis. The albumin peptide prepared by the present invention has a small molecular weight, is easy to digest and absorb, and has the effects of promoting growth and development, increasing the proliferation of macrophages, and enhancing immunity, etc.

This invention provides a postbiotic immunomodulatory agent based on *Bifidobacterium longum* CD11 and its preparation method. The postbiotic is prepared using *Bifidobacterium longum* CD11. Heat-inactivated metabolites of *Bifidobacterium longum* CD11, cell wall, cell membrane, and cell contents postbiotic components all promote the immune activity of RAW264.7 macrophages. Specifically, the cell wall component dose-dependently promotes RAW264.7 macrophage proliferation and inhibits the relative expression of inflammatory cytokines TNF-α and IL-1β mRNA genes. Furthermore, the peptidoglycan (PGN) postbiotic derived from the cell wall component significantly inhibits the relative expression of TNF-α and IL-1β mRNA genes in RAW264.7 macrophages, restoring inflammation to a healthy level (NC group level). Therefore, *Bifidobacterium longum* CD11 can serve as a potential immunomodulatory postbiotic strain.

The invention belongs to the technical field of biological medicine, and particularly discloses tremella aurantialba polysaccharide with immunomodulatory activity and a preparation method and application thereof. The preparation method comprises the following steps: carrying out water extraction and alcohol precipitation on tremella aurantialba, and carrying out column chromatography separation to obtain tremella aurantialba polysaccharide TAP-2a; meanwhile, the structure of the TAP-2a is determined by adopting methods such as high performance gel permeation chromatography, precolumn derivatization, methylation combined with gas chromatography-mass spectrometry, nuclear magnetic resonance and the like, and the obtained TAP-2a is brand new tremella aurantialba polysaccharide. Meanwhile, the immunoregulation activity of the TAP-2a is further researched, the TAP-2a can promote proliferation of macrophages, up-regulate expression of iNOS, TNF-alpha, IL-6 and IL-1beta mRNA, promote secretion of NO, TNF-alpha, IL-6 and IL-1beta and enhance the phagocytic ability of the macrophages, and the result shows that the polysaccharide has the immunoregulation activity, can be used as a safe natural immunomodulator and has wide application prospects.

An siRNA specifically binding to M2 macrophage CD206 is provided, which is at least one of siRNA-1, siRNA-2, and siRNA-3, or at least one of siRNA-4, siRNA-5, and siRNA-6. The siRNA specifically combined with CD206 of the M2 macrophage reduces the expression of CD206 by specifically binding to CD206 of the macrophage, thereby promoting the proliferation of the M2 macrophage and further inhibiting the proliferation and migration of tumor cells.