14 results about "Macrophage proliferation" patented technology

The invention discloses flammulina velutipes polysaccharide nano-liposome as well as a preparation method and application thereof, and belongs to the technical field of biological functional materials. The preparation method specifically comprises the following steps: (1) sequentially performing hot water extraction, deproteinization, alcohol precipitation, dialysis and freeze-drying on flammulina velutipes raw material powder; (2) dissolving in a phosphoric acid buffer solution; (3) mixing and dissolving phospholipid, cationic lipid and a membrane regulator in an organic solvent; (4) mixing and ultrasonic treatment; and (5) carrying out reduced-pressure rotary evaporation, hydration with a phosphate buffer solution, ultrasonic treatment and extrusion. According to the flammulina velutipes polysaccharide nano-liposome and the preparation method thereof, cholesterol is replaced by phytosterol ester to prepare the flammulina velutipes polysaccharide nano-liposome, flammulina velutipes polysaccharide (FVP) is encapsulated in a water core in the liposome, the safety is higher, and the encapsulation efficiency, the storage stability and the immunocompetence of a system are improved. In-vitro experiments prove that the phytosterol ester lipidosome has the effect of promoting macrophage proliferation and nitric oxide generation, and the effect is obviously better than that of the traditional cholesterol lipidosome under the same condition.

The invention belongs to the technical field of traditional Chinese medicine extraction, and relates to a nine-steamed nine-processed rhizoma polygonati homogeneous polysaccharide and a preparation method and application thereof, in particular to a nine-steamed nine-processed rhizoma polygonati homogeneous polysaccharide and a preparation method and application thereof. The purpose of the invention is to determine one component in the nine-steamed nine-processed polygonatum sibiricum product. NPCP-A1 is extracted by a water extraction and alcohol precipitation method, deproteinized by a Sevag method, and subjected to chromatographic separation and purification by a DEAE-52 anion exchange column and a Sephacryl S-300 gel column, and the NPCP-A1 is prepared from arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid. The proliferation of RAW264.7 cells and the secretion of NO, TNF-alpha and IL-6 can be promoted. The composition is used for preparing health-care products or medicines for improving immunity. According to the acidic polysaccharide prepared by the method disclosed by the invention, the polysaccharide plays an immune regulation role by promoting macrophage proliferation and increasing secretion of cell factors such as NO, TNF-alpha, IL-6 and the like.

This invention discloses a freeze-dried preparation process of a Cordyceps and Polyporus umbellatus antitumor complex, specifically relating to the field of biomedical technology. Specifically, it uses Cordyceps powder and Polyporus umbellatus powder as raw materials, employing a simulated intestinal solution for repeated low-temperature pulsed ultrasound extraction under specific pH conditions. Following low-temperature clarification, filtration, reduced-pressure concentration, membrane fractionation, and freeze-drying protection, a freeze-dried antitumor complex powder is obtained, exhibiting both active ingredient retention, reconstitution stability, and the ability to promote macrophage proliferation and inhibit tumor cell proliferation. This invention, through the synergistic effect of intestinal simulated solution extraction, low-temperature pulsed ultrasound, membrane fractionation, and freeze-drying protection, fully releases and stably retains the active ingredients of Cordyceps and Polyporus umbellatus, improving extraction efficiency, reconstitution, and storage stability, enabling the freeze-dried powder to simultaneously promote macrophage proliferation and inhibit tumor cell proliferation.

The invention provides a postbiotic immunomodulatory preparation based on bifidobacterium longum CD11 and a preparation method of the postbiotic immunomodulatory preparation. Bifidobacterium longum CD11 is used to prepare a metagen. The immunocompetence of RAW264.7 macrophages can be promoted by thermal inactivation of bifidobacterium longum CD11 metabolites, cell walls, cell membranes and cell content metabolite components, and the cell wall component dose-dependently promotes the proliferation of the RAW264.7 macrophages and inhibits the relative expression of mRNA genes of inflammatory factors TNF-alpha and IL-1beta. In addition, a peptidoglycan (PGN) metagen from a cell wall component can remarkably inhibit relative expression of TNF-alpha and IL-1beta mRNA genes of RAW264.7 macrophages, so that inflammation is recovered to a health level (NC group level). Therefore, the bifidobacterium longum CD11 can be used as a potential immunomodulatory metabiotic strain.

The invention discloses a tuna red meat immunity enhancing peptide and application thereof in enhancing immunity, and belongs to the technical field of biology. According to the invention, polypeptides with immunocompetence are extracted from tuna red meat, and the polypeptides comprise FLQGF and LAKFPDF. Experiments prove that the polypeptide prepared by the invention has no obvious toxic effect on cells, and can improve the proliferation rate and the phagocytic rate of macrophages and the release rate of NO to different degrees. An in-vitro immunomodulatory activity result shows that the LAKFPDF and the FLQGF can activate a TLR4 dependent NF-kB / MAPK signal channel, regulate and control the immune response of macrophages and play an immunomodulatory role. Research results of the invention provide a basis for high-value development and utilization of tuna red meat, and provide a new resource for preparation of immunopotentiation products.

A method of producing macrophages includes a culture step of culturing hematopoietic progenitor cells in the presence of a TREM2 signal activator.

The invention belongs to the technical field of biological medicines, and particularly relates to a method for promoting proliferation of immature bone marrow-derived macrophages and polarization of the immature bone marrow-derived macrophages to M1 through stress application culture and application of the method. The in-vitro stress application culture method of the macrophages from the immature bone marrow has the characteristics of high stability, high amplification efficiency, polarization controllability, high purity, flexibility in application, low production cost and the like; the problems that a traditional cell factor induction method is low in amplification efficiency, poor in polarization controllability, insufficient in purity and the like are solved, the purity of the obtained M1 type macrophage reaches 90% or above, stability is high, and cost is low. The method can be applied to preparation of macrophage drugs for immunoregulation, anti-fibrosis, metabolism regulation, anti-tumor CAR-M and the like, tissue regeneration biological materials, anti-inflammatory drugs, in-vitro diagnosis products and the like, dosage forms are diversified, and a standardized cell preparation scheme is provided for the related biological medicine field.

This invention belongs to the field of biotechnology, specifically relating to a highly immunomodulatory peptide derived from silkworm pupa protein, its preparation method, and its applications. The invention provides a silkworm pupa protein immunomodulatory peptide, the amino acid sequence of which is shown in SEQ ID NO.2, SEQ ID NO.3, and / or SEQ ID NO.8. This silkworm pupa protein immunomodulatory peptide is prepared by hydrolyzing silkworm pupa protein with alkaline protease, followed by purification of the enzymatic hydrolysis product through ultrafiltration and gel filtration. The most immunomodulatory peptide is screened by determining the immunomodulatory activity index, and identified by LC-MS / MS to obtain a highly immunomodulatory peptide derived from silkworm pupa protein. This immunomodulatory peptide strongly binds to TLR2 and TLR4 / MD-2 receptors through hydrogen bonds and hydrophobic interactions, activating immunity and effectively promoting macrophage proliferation and phagocytosis. This immunomodulatory peptide exhibits good immunomodulatory activity and can be used as a potential functional food ingredient.

The present application belongs to the technical field of biological medicine, and relates to a kind of Parachlorella sp. Polysaccharide and its preparation method and application. The present application obtains a kind of uniform Parachlorella sp. Polysaccharide NGP-1 by water extraction, alcohol precipitation, column chromatography separation to Parachlorella sp.;Combining high performance liquid chromatography, methylation combined gas chromatography-mass spectrometry, nuclear magnetic resonance and other methods to determine its structural characteristics. The obtained NGP-1 can promote macrophage proliferation, enhance phagocytic ability, improve active oxygen level, induce nitric oxide and cytokine production, and up-regulate the expression of corresponding mRNA, and has significant immune enhancing activity. The Parachlorella sp. Polysaccharide NGP-1 prepared by the present application can be used as a natural immune enhancer, which widens the application range of Parachlorella sp. And has broad application prospect.

To provide a macrophage production method capable of yielding macrophages that have a prolonged survival rate, allow for freezing preservation, and offer versatility as a tool in scientific studies.SOLUTION: A method for producing macrophages comprises a culture step for culturing hematopoietic precursor cells in the presence of a TREM2 signal activator.SELECTED DRAWING: Figure 1

The anti-inflammatory composition promotes proliferation of macrophage and suppresses inflammation caused by inflammatory cytokine and / or NO synthase, and promotes proliferation of human fibroblast or human epidermal keratinocyte and promotes synthesis of hyaluronic acid in epidermis and dermis. To provide a skin-beautifying and promoting composition which keeps the tension of the skin, strengthens the bonding force of the skin, improves the barrier function of the skin, enhances the strength and water-retaining function of the skin surface, or promotes the anti-aging of the skin, to provide a hair-growing composition which promotes the proliferation of human epidermal keratinocyte cells, improves the strength of the skin surface, and promotes hair growth, and to provide cosmetic compositions thereof.SOLUTION: The composition comprises a high-pressure treated product of a mixture of sturgeon eggs and 1, 3-butylene glycol.SELECTED DRAWING: Figure 3

The application discloses application of a large-mouth black bass stomach polypeptide in preparation of an antioxidant and / or immunoregulation product, relates to the field of bioactive peptides, and is characterized in that the large-mouth black bass stomach polypeptide is a polypeptide product obtained by alkaline protease enzymolysis of large-mouth black bass stomach, and components with molecular weights less than 3000 Da account for more than 98% of the total peptide amount. The polypeptide has significant DPPH free radical, hydroxyl free radical and ABTS cationic free radical scavenging capacity and reducing capacity, can promote macrophage proliferation, enhance phagocytosis, promote nitric oxide secretion, up-regulate immune-related cytokine expression, and play an immunoregulation role through activation of P38 and P44 MAPK signal pathways. The application realizes high-value utilization of large-mouth black bass processing by-products, has high product safety, and has a wide application prospect.

The invention discloses a method for preparing ulva pertusa polysaccharide through co-fermentation of bacillus velezensis SCAU-104 and ulva pertusa powder and application of the ulva pertusa polysaccharide. Compared with the traditional hot water extracted polysaccharide (UPP), the polysaccharide (UBV) obtained by fermentation extraction has the advantages of lower molecular weight, higher sulfate group retention and more optimized structure. And carrying out macroporous resin deproteinization, ultrafiltration desalination and chromatographic purification on the UBV, and separating to obtain the uniform polysaccharide UBV-1. Researches show that UBV mainly comprises rhamnose, galactose and glucuronic acid, and is single molecular weight acidic sulfated polysaccharide. An in-vitro experiment shows that the UBV remarkably promotes RAW264.7 macrophage proliferation and NO and proinflammatory factor release, and the immunocompetence of the UBV is superior to that of UPP. The method disclosed by the invention is green and controllable, and the obtained polysaccharide is high in purity and strong in activity and can be widely applied to development of functional foods, cosmetics, skin care products and immunomodulatory products.

The invention belongs to the technical field of biological medicine, and particularly discloses tremella aurantialba polysaccharide with immunomodulatory activity and a preparation method and application thereof. The preparation method comprises the following steps: carrying out water extraction and alcohol precipitation on tremella aurantialba, and carrying out column chromatography separation to obtain tremella aurantialba polysaccharide TAP-2a; meanwhile, the structure of the TAP-2a is determined by adopting methods such as high performance gel permeation chromatography, precolumn derivatization, methylation combined with gas chromatography-mass spectrometry, nuclear magnetic resonance and the like, and the obtained TAP-2a is brand new tremella aurantialba polysaccharide. Meanwhile, the immunoregulation activity of the TAP-2a is further researched, the TAP-2a can promote proliferation of macrophages, up-regulate expression of iNOS, TNF-alpha, IL-6 and IL-1beta mRNA, promote secretion of NO, TNF-alpha, IL-6 and IL-1beta and enhance the phagocytic ability of the macrophages, and the result shows that the polysaccharide has the immunoregulation activity, can be used as a safe natural immunomodulator and has wide application prospects.