Preparation method and application of small-molecule panax notoginseng polysaccharide extract

Abstract

The invention discloses a preparation method of small-molecule panax notoginseng polysaccharide extract. The preparation method includes: using panax notoginseng as the raw material, crushing the raw material, extracting with an ethanol solution, extracting panax notoginseng residues with water obtain crude panax notoginseng polysaccharide extract, performing ethanol precipitation, dissolving precipitate with water, and then adding trifluoroacetic acid or lysozyme to perform hydrolysis to obtain crude small-molecule polysaccharide liquid; using a cation-anion exchange column to remove the protein, pigment and the like of the crude small-molecule polysaccharide liquid, using activated carbon powder for decoloring, filtering with a titanium-bar filter to obtain a colorless low-viscosity small-molecule polysaccharide solution, concentrating, and performing freeze drying to obtain the small-molecule panax notoginseng polysaccharide extract. The preparation method has the advantages that the method is simple to operate, low in cost and high in industrialization degree; the small-molecule panax notoginseng polysaccharide extract can promote macrophage proliferation, further differentiation and maturation of macrophage can be achieved to obtain activated macrophage, and the phagocytic ability of the macrophage can be evidently enhanced; fast increasing of cell immunity during a cell culture immunity test can be achieved; the extract has application prospect in immunological disease treatment, and the preparation method is suitable for industrial production.

Description

technical field [0001] The invention relates to a preparation method and application of a small-molecule Panax notoginseng polysaccharide extract, belonging to the field of extraction and separation of plant active substances. Background technique [0002] Many reports in the study of plant sugar chemistry point out that the highly active polysaccharides with curative and disease-preventive effects are basically small molecular polysaccharides, and the efficacy of macromolecular polysaccharides is relatively weak. The immune system is an extremely complex and important physiological system, in which phagocytes are one of the body's first lines of defense against infection by pathogenic microorganisms, including neutrophils, monocytes, macrophages, etc., which are all innate immunity important component of the system. Among them, macrophages can non-specifically phagocytize and kill pathogenic microorganisms, and at the same time release some inflammatory mediators and cytok...

AI Technical Summary

Problems solved by technology

[0004] There is no report on the extraction method of the active small-molecule polysaccharides of Panax notoginseng, and the relevant reports mainly focus on the extraction method and pharmacological activity of the total polysaccharides of Panax notoginseng; The total polysaccharides of Panax notoginseng are obtained by precipitation, deproteinization, and drying. However, since the extracted macromolecular polysaccharides contain soluble cellulose, starch, etc., the medicinal activity is very low, and most of the macromolecular polysaccharides have molecular weights of tens of millions or millions. , the human body is difficult to absorb, and the human body needs to be metabolized into small molecular components in order to truly absorb and function, so the utilization of macromolecular polysaccharides is low