108results about How to "Inhibit kill" patented technology

The invention relates to a method for local fixed-point control on soil-borne disease of vegetables. According to the method, two measures, i.e., medicated seedling raising bags and medicated matrixes are combined, the medicated matrixes are loaded into the medicated seedling raising bags, seedling raising is carried out after watering, and young seedlings are transplanted to greenhouses or fields together with the medicated matrixes and the medicated seedling raising bags during fix-planting. On one hand, stem bases and root upper parts, which are susceptible to infection, of the young seedlings of the vegetables are protected by the medicated matrixes and the medicated seedling raising bags and are isolated from germ-carrying soil, so that a long-lasting protective effect on roots of the vegetables is achieved, and the effect of preventing and lowering the frequency of occurrence of disease for the roots of the vegetables is achieved; and on the other hand, the growth of the roots of the vegetables is free from the restriction of the seedling raising bags, and the roots of the vegetables can penetrate through the seedling raising bags and freely extend towards the fields, so as to achieve the aim of local fixed-point control on the soil-borne disease of the vegetables. The medicated seedling raising bags and the medicated matrixes are combined with the method for local fixed-point control on the soil-borne disease of the vegetables, and one-time operation is required only during sowing, so that the method is simple and convenient and is low in cost and remarkable in effect; and the consumption of pesticides can be reduced, the environmental safety is enhanced, the labor intensity for farmers is lowered, and the popularization is facilitated, so that the method is a first-choice effective prescription for the prevention and treatment of root disease during the production of pollution-free vegetables and the production of green vegetables.

The invention discloses a tetrastlgma hemsleyanum vine polysaccharide extract, a preparation method and application thereof. The method includes: crushing dry tetrastlgma hemsleyanum vine to obtain dry powder, conducting extraction with water, performing filtering, and drying the filtrate to obtain a tetrastlgma hemsleyanum vine aqueous extract, and subjecting the tetrastlgma hemsleyanum vine aqueous extract to ethanol precipitation and two-aqueous phase extraction, thus obtaining the tetrastlgma hemsleyanum vine polysaccharide extract with a polysaccharide content of 53.7%-97.8%. The tetrastlgma hemsleyanum vine polysaccharide extract prepared by the method provided by the invention has antibacterial and bactericidal effects, and can be used for preparation of bacteriostatic and bactericidal articles. If the tetrastlgma hemsleyanum vine polysaccharide extract is prepared into an antibacterial liquid medicine, the medicine liquid can be used as a component in drugs, cosmetics, health food, food and used for bacteriostasis and antibiosis in people, animals and aquaculture process. Besides the original public efficacy of tetrastlgma hemsleyanum, the tetrastlgma hemsleyanum vine polysaccharide extract has new use in drugs, cosmetics, health food and food, and has bacteriostatic and bactericidal effects, thus having broad application prospects.

The invention relate to a nm material modified latex coating for inside wall with environmental protection,comprises of the following components:water 18-24,dispersant 0.4-1.0;nm silicon dioxide powder 1.0-3.0;deposit barium sulfate 20-30;talcum powder 10-15;titanium dioxide 10-20;antifoaming agent 0.1-0.2;modified crylic acid 25-35;zinc oxide and titanium oxide compound finishing mixture 0.1-0.3;thickener 0.3-0.4; 0.15-0.30;and pH regulator. The modified crylic acid has the lowest temperature to form film being 0-5deg.C and glassifying temperature being 10-15deg.C, the coating contains 6 organic compound lower than 5g/l,is suitable for decoration and protection for inside wall.

The invention provides a recombinant human hyaluronic acid composition which includes, by weight, 0.001-9.0% of recombinant human hyaluronic acid, 0.2-20.0% of a plant compound, 0.05-10.0% of a skin conditioner, and 61.0-99.7% of a matrix auxiliary material. The recombinant human hyaluronic acid is prepared by cutting large-molecular hyaluronic acid by means of saccharified recombinant human hyaluronidase PH20 produced from CHO cells. At least one end of the recombinant human hyaluronic acid has a terminal which is cut by a recombinant human hyaluronidase capable of recognizing the normal structure of hyaluronic acid. A cosmetic prepared from the recombinant human hyaluronic acid composition can promote skin mucosa to secrete functional components; in addition, active components in the cosmetic are quickly diffused and permeate into interior of cells.

The invention discloses an ethanol extract of radix tetrastigmae leaves as well as a preparation method and application of the ethanol extract. The preparation method comprises the steps of smashing dried radix tetrastigmae leaves to obtain dry powder; extracting by using an ethanol water solution, and filtering to obtain filtrate; and drying the filtrate to obtain the ethanol extract of the radix tetrastigmae leaves. According to the preparation method, bacterium inhibiting and killing components in the radix tetrastigmae leaves are extracted through extracting by using the ethanol water solution, and finally, the ethanol extract of the radix tetrastigmae leaves is obtained. The ethanol extract of the radix tetrastigmae leaves, prepared by using the preparation method, can be used for preparing bacterium inhibiting and killing supplies, can be used for inhibiting and killing bacteria on drugs, cosmetics, health foods and foods as well as bacteria generated in human, animals and aquaculture, and can be used for effectively inhibiting bacterial growth and killing bacteria.

The invention discloses an efficient environmentally-friendly compound feed for groupers. The compound feed comprises the following components in percentage by weight: 35-45% of white fish meal, 5-10% of squid powder, 6-14% of Antarctic krill powder, 10-20% of peeled soybean meal, 7-15% of vital wheat gluten, 6-12% of strong flour, 0.3-1% of calcium dihydrogen phosphate, 2-6% of fish oil, 1-3% of soybean oil, 1-3% of liquid phosphatide, 0.1-0.3% of bacillus, 0.2-0.5% of tributyrin, 1% of a vitamin premix, and 1% of a mineral substance premix. According to the compound feed disclosed by the invention, the health of intestinal tracts of the groupers can be improved, the damage of the intestinal tracts is avoided, the digestive absorption rate of the feed can be increased, the feed coefficient is reduced, and the discharge of nitrogen is reduced, so that the purpose that the compound feed is efficient and environmentally-friendly is achieved.

The present invention relates to red tide and algalbloom preventing and controlling technology, and is especially method of adopting glycolipid surfactant in treating red tide of sea water and algalbloom of fresh water. The effective component of the biological surfactant is sophor glycolipid as one kind of mixture comprising one acid type matter and one lactone type matter. It may be used alone to inhibit and kill harmful alga and crack their cells effectively and the effective concentration for eliminating alga in different kinds is 5-10 mg/l. It may be also used through mixing with clay mineral to raise the flocculant efficiency organic clay mineral obviously, and the optimal mixing weight ratio to loess is 1 to 100-200. The present invention has easy biodegradation, no harm to other life beings, no secondary pollution and easy storing.

The invention relates to the technical field of medicine, and provides application of luteolin in a bacterial quorum sensing inhibition system, application of luteolin in preparing drug for lowering pathogenicity of staphylococcus aureus virulence factors and application of luteolin in preparing drug for inhibiting transcription of hla and agrA genes of staphylococcus aureus. Minimum effective concentration with application value is provided for the application of luteolin in the bacterial quorum sensing inhibition system, and a reference is provided for medically preparing specific bio-film forming inhibitors. The invention further provides a drug combination for inhibiting formation of staphylococcus aureus bio-films. The drug combination contains luteolin. According to the drug combination, formation of the bio-films of staphylococcus aureus can be inhibited by effectively inhibiting a quorum sensing system of staphylococcus aureus through tiny luteolin, prognosis risk, of staphylococcus aureus, for infection with high-drug-tolerance bio-films in anti-infection treatment is lowered, and a valuable reference is provided for medically preparing specific preparations for inhibiting the staphylococcus aureus bio-films.

The invention provides garlic oil soft capsules and a preparation method thereof. The preparation comprises following steps: the peel of garlic which has no moldy or rotten parts and has buds is removed; the garlic is added into a tissue grinder and is grinded into pulp; 18 to 22kg of the garlic pulp is added into an enzymolysis tank, 0.4 to 0.7kg of hemicellulase is added, the mixture is subjected to enzymolysis for 2.8 to 3.2h at a temperature of 42 to 47 DEG C so as to obtain a garlic enzymatic hydrolysate; the garlic enzymatic hydrolysate is added into an extractor, the steel cylinder of the extractor is turned on so as to delivery CO2 into the extractor, the extractor is pre-heated to 33 to 37 DEG C, and the garlic enzymatic hydrolysate is subjected to extraction for 2.8 to 3.3h, wherein the pressure is 16 to 19MPa, and the ratio of the flux of an extraction agent to the flux of the sample material is 5-6LCO2/h.g galic; the garlic oil is obtained after separation; and then a full-automatic capsule filling machine is used for preparation of the garlic oil soft capsules, wherein the weight of each garlic oil soft capsule is 0.2g. Dosage and administration are that: the garlic oil capsules are taken orally, 2 to 3 times a day, and 1 to 2 capsules each time; and the garlic oil capsules should be taken after meal.

The invention relates to a 4-borneol aldehyde benzoate, a preparation method thereof and an antibacterial application, The 4- borneol aldehyde benzoate takes borneol and 4-aldehyde benzoic acid as rawmaterials to form a borneol esterified derivative as an antibacterial microbial substance by a one-step esterification reaction. The 4-borneol aldehyde benzoate has strong antibacterial effect on gram-positive bacteria (such as Staphylococcus aureus, Bacillus subtilis) and gram-negative bacteria (such as pseudomonas aeruginosa, acinetobacter baumannii), and has good biocompatibility and non-toxicity. In addition, the raw materials and reagents of the invention are easy to obtain, the preparation method is simple, and the products have broad application prospects in the fields of medicine, food, health and the like.

The invention provides hyaluronic acid BB cream with multiple effects. The composition comprises the following ingredients by weight percent: 0.01-3.0% of hyaluronic acid, 1.0-5.0% of plant sucrose clear paste, 0.5-3.5% of a function compound and 89.0-98.4% of substrate auxiliary materials, wherein the plant sucrose clear paste is prepared by mixing eight active plants of an aloe smooth agent, herba leonuri, horse chestnut, lophatherum gracile, white willow barks, portulacae herba, dracaena draco linn and witch hazel and sucrose solution; the function compound is prepared by mixing nicotinamide, allantoin and polyglutamic acid at a ratio. The hyaluronic acid BB cream not only has the basic effects of covering, adjusting skin color, sunscreening, and refining pores, but also has the multiple effects of preserving moisture, brightening skin tone, being anti-allergy, diminishing inflammation, being antibacterial and anti-aging and the like; meanwhile, the hyaluronic acid BB cream is natural and simple in ingredients, excellent in water resistance and strong in setting capacity, so that the hyaluronic acid BB cream is a novel hyaluronic acid BB cream which is safe, practical, durable and stable and has the multiple effects.

The invention discloses a separating mediumon solution for removing dead hepatocytes and a method for removing dead hepatocytes by utilizing the separating mediumon solution and belongs to the technical field of hepatocyte isolationliver cell separation and purification. The separating mediumon solution is prepared by adding the following components in a William's E culture medium: 0.1-1.5 percentv/v of ITS universal culture additives, 1.5-2.5 mmol/L of L-glutamine, 1-20 [mu]gmu g/L of an epidermal growth factor, 15-20mg/L of hydrocortisone, 35-45 [mu]gmu g/L of dexamethasone, 0.5-1 percent v/v of penicillin streptomycin, 1-10 percent v/v fetal calf serum and 0.25-0.5g/mL of Nycodenz. The separating medium tion solution has the advantages of maintaining normal osmotic pressure of hepatocytes, repairing partial damaged hepatocytes and providing nutritional requirement for hepatocytes.

The invention discloses a high-yield planting method of honey peaches, pertaining to the technical field of plantation of honey peaches. The method comprises the following steps: (1) land selection and preparation; (2) seedling selection; (3) fixed planting; (4) top dressing; (5) management of trees; (6) prevention and control of pests and diseases; (7) harvesting. The high-yield planting method of honey peaches has the following beneficial effects: the method is reasonable and scientific; mouth feel of honey peaches is improved; and quality and output of honey peaches are improved.

The invention discloses a hippophae rhamnoides extract for treating breast cancer and liver cancer as well as a preparation method and application of the hippophae rhamnoides extract. The hippophae rhamnoides extract is prepared by the following steps: 1) drying hippophae rhamnoides fruits, so that dried hippophae rhamnoides fruits are obtained; 2) crushing the dried hippophae rhamnoides fruits to 100-800 meshes so as to obtain hippophae rhamnoides powder; 3) at 4-25 DEG C, soaking the hippophae rhamnoides powder in water for 1-10h, and ultrasonically extracting for 10min-6h so as to obtain a hippophae rhamnoides extracting solution, wherein the mass-volume ratio of the hippophae rhamnoides powder to the water is 1 to (2-15); and 4) filtering, collecting the hippophae rhamnoides extracting solution and drying the extracting solution, so that the hippophae rhamnoides extract is obtained. The inhibitory rate of the extract on breast cancer cells reaches 80.73% and the inhibitory rate on liver cancer cells reaches 87.28%, and the hippophae rhamnoides extract is low in toxic and side effects.

The invention provides an efficient gene knockout vector construction method. According to the present invention, an efficient negative screening marker gene and a USER enzyme cloning technology are used to construct an enzyme cutting site in a vector so as to make the vector generate a sticky end after the enzyme cutting, the efficient negative screening marker gene is constructed in the vector, and the constructed vector is successfully used for knocking-out Verticillium dahliae V592 target gene, wherein the sticky end can be complementary to the upstream fragment and the downstream fragments of the target gene obtained after the enzyme cutting with the USER enzyme; and the experiment results prove that the research period of the Verticillium dahliae target gene is substantially shortened and the Verticillium dahliae function gene research is accelerated with the vector constructed by using the method of the present invention.

The invention relates to the field of medical instruments, and particularly discloses a positioning device for blood drawing. The positioning device comprises a positioning rod, a positioning plate and a limiting unit, the positioning plate is opposite to the needle head, and the positioning rod is located between the positioning plate and the needle head and perpendicular to the moving directionof the needle head; the limiting unit is located on the side, away from the needle head, of the positioning rod and comprises a limiting plate capable of sliding towards the side close to the needle head and a driving piece used for driving the limiting plate to slide, and the limiting plate is opposite to the positioning rod. According to the positioning device for blood drawing, the arm of a patient can be fixed, so that a needle head can conveniently position a blood vessel.

The invention relates to a preparation method of a sterilizing and disinfecting agent used for producing edible mushrooms. The sterilizing and disinfecting agent is composed of urea hydrogen peroxide, dimethyl fumarate, hexamethylenetetramine, Tween (TW-80), Span (SP-20) and sodium lauryl sulfate (K12). The sterilizing and disinfecting agent is mixed with a culture medium according to a certain ratio when being used so that harmful virus microorganisms can be effectively inhibited; a sterilization temperature is reduced and the sterilization time is shortened; the loss of nutritional ingredients is reduced and energy sources are saved; a microenvironment for growth and development of mycelia is improved; and an efficient, broad-spectrum and residue-free good sterilizing and disinfecting agent composition which is convenient to use is provided.

The invention discloses a feed for young carps and a preparation method of the feed. The feed for young carps comprises the following components in parts by weight: 15-20 parts of fish meal, 35-45 parts of hermetia illucens larvae, 18-25 parts of wheat bran, 10-15 parts of tomatoes, 18-25 parts of corn, 5-10 parts of hericium erinaceus, 8-15 parts of asparagus, 3-8 parts of garlic, 5-10 parts of black wood ears, 3-7 parts of complex vitamins, 5-10 parts of silkworm chrysalis, 3-8 parts of yeast, 15-25 parts of maifan stones, 1-5 parts of heartleaf houttuynia herbs and 0.5-1.5 parts of minerals. The preparation method comprises the following steps of (1) preparing hermetia illucens larva powder and silkworm chrysalis meal; (2) crushing the tomatoes, the corn, the hericium erinaceus, the asparagus, the garlic, the black wood ears and the heartleaf houttuynia herbs, and then performing fermentation to prepare fermented slurry; (3) performing mixed granulation on the hermetia illucens larva powder, the silkworm chrysalis meal, the fermented slurry, the fish meal, the maifan stones and the minerals; and (4) performing sterilization and performing packing. The feed can improve the immunity of the young carps.

The invention discloses a high efficiency bactericide used for domestic fungi. The high efficiency bactericide comprises 20-30% of sodium dichloroisocyanurate, 40-60% of calcium oxide and 20-30% of calcium hypochlorite. The bactericide has the advantages of effective inhibiting and killing of harmful microbes, reduction of the base number of microbes in a medium, reduction of the sterilization cost and the loss caused by loss in the production process of the domestic fungi, and strong mycelium growth promotion effect.