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Nisin polylysine antibacterial nano particles and preparation method thereof

A nanoparticle and polylysine technology, which is applied in the field of food antibacterial additives, can solve the problems of reducing stability and biological activity, and achieve the effects of good product stability, special product structure and small particle size

Inactive Publication Date: 2018-02-09
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a polypeptide substance, Nisin will bond or enzymatically react with food ingredients if it is directly applied to food, thereby reducing its stability and biological activity

Method used

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  • Nisin polylysine antibacterial nano particles and preparation method thereof
  • Nisin polylysine antibacterial nano particles and preparation method thereof
  • Nisin polylysine antibacterial nano particles and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The specific implementation of the present invention is illustrated by the following examples, but the protection content of the present invention is not limited thereto. The preparation of embodiment 1Nisin polylysine antibacterial nanoparticles

[0024] 1 Experimental materials

[0025] Nisin potency 1100IU / mg Luoyang Qihong Biotechnology Co., Ltd.

[0026] γ-PGA Mw 50,000-100,000 Shandong Freda Biotechnology Co., Ltd.

[0027] PLL Mw 150,000-300,000 Shanghai Yuanye Biotechnology Co., Ltd.

[0028] 2 Experimental methods

[0029] The preparation steps of Nisin polylysine antibacterial nanoparticles are as follows:

[0030] ①Use deionized water to prepare Nisin solution (2.0mg / mL), γ-PGA solution (3.0mg / mL) and PLL solution (2.0mg / mL).

[0031] ② Mix Nisin solution (2.0mg / mL) and γ-PGA solution (3.0mg / mL) in equal volumes, and stir the mixture on a magnetic stirrer (200rpm) for 2h at room temperature.

[0032] ③Add an equal volume (equal volume to Nisin solution)...

Embodiment 2

[0034] The characterization of embodiment 2Nisin polylysine antibacterial nanoparticles

[0035] 1 Experimental materials

[0036] Nisin polylysine antibacterial nanoparticles

[0037] 2 Experimental materials and instruments

[0038]

[0039] 3 Experimental methods

[0040] ①The particle size and polydispersity index (PDI) of Nisin polylysine antibacterial nanoparticles were measured by laser particle size analyzer (Nano ZS90). The Zeta potential of antibacterial nanoparticles was measured with a potentiometer (Nano ZS90Zeta).

[0041] ②The encapsulation efficiency of Nisin in the antibacterial nanoparticles was determined indirectly by BCA method trace protein detection kit. During the preparation of Nisin polylysine antibacterial nanoparticles, the supernatant was collected, and the concentration of Nisin in the supernatant (that is, the concentration of unencapsulated Nisin in the nanoparticles) was determined using a BCA method trace protein detection kit. Utilize...

Embodiment 3

[0049] Nisin release performance evaluation in embodiment 3Nisin polylysine antibacterial nanoparticles

[0050] 1 Experimental materials

[0051] Nisin polylysine antibacterial nanoparticles

[0052] 2 experimental equipment

[0053] BCA Trace Protein Detection Kit / Nanjing Jiancheng Bioengineering Co., Ltd.

[0054] Dialysis bag 7000Da Beijing Solaibao Technology Co., Ltd.

[0055] 3 Experimental methods

[0056] Weigh a certain amount of Nisin polylysine antibacterial nanoparticles, put it into a dialysis bag (molecular cut-off 7000Da), then put the dialysis bag in 100.0mL PBS, and place it on a magnetic stirrer to rotate slowly (100 rpm) . At regular intervals, 500 μL of samples were taken out, and an equal amount of PBS was added after sampling to maintain a constant solution volume. The sample taken out was centrifuged (6000rpm / min) for 15min, and the supernatant was taken, and the concentration of Nisin in the supernatant was determined using a BCA method trace p...

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Abstract

The invention belongs to the field of antibacterial additives for foods, and particularly relates to Nisin polylysine antibacterial nano particles high in stability and high baceterium resistance anda preparation method of the Nisin polylysine antibacterial nano particles. According to the Nisin polylysine antibacterial nano particles disclosed by the invention, the characteristics of a charged type natural polymer are used, a static electricity self-assembly technique is adopted, and Nisin embedding polylysine antibacterial nano particles are prepared, so that Nisin can be prevented from generating a bonding reaction or an enzymolysis reaction with food components in practical application, and the stability and the bioactivity of the Nisin can be effectively maintained. The Nisin polylysine antibacterial nano particles disclosed by the invention are simple in preparation technology, good in product stability, uniform in particle size distribution, high in entrapment efficiency and high in drug loading capacity, and have remarkable antibacterial effects on listeria monocytogenes (L.monocytogenes) and staphylococcus aureus (S.aureus) in simulated condensed milk culture mediums.

Description

technical field [0001] The invention belongs to the field of food antibacterial additives, and in particular relates to a Nisin polylysine antibacterial nanoparticle and a preparation method thereof. Background technique [0002] Nisin, also known as nisin or Nisin, is a polypeptide substance produced by Lactococcus lactis subspecies lactic acid, which belongs to microbial antibacterial substances. It has broad-spectrum antibacterial effect on Gram-positive bacteria, and is natural, safe and efficient. As a polypeptide substance, Nisin will bond or enzymatically react with food ingredients if it is directly applied to food, thereby reducing its stability and biological activity. [0003] In recent years, nanoparticles, as drug delivery carriers, have attracted extensive attention from researchers due to their small particle size and large specific surface area. Biopolymers (such as polyglutamic acid, polylysine, chitosan, and pectin, etc.) are widely used in the preparatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L3/3526
CPCA23L3/3526
Inventor 林琳代娅婕崔海英王莹
Owner JIANGSU UNIV
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