11 results about "Nisin" patented technology

This invention discloses a lactide-Nisin solid dispersion and its preparation method. The method includes preparing a mixture of lactide and a hydrophobic solvent; dispersing Nisin in the mixture obtained in the previous step to obtain a multi-component mixture; and cold-treating the multi-component mixture to obtain a solid mixture containing lactide and Nisin. This invention fully utilizes the binding properties of lactide and Nisin, effectively enhancing the stability of Nisin in complex environments, further expanding the application potential of the prepared antibacterial agent, and providing a new approach for developing multifunctional green food preservatives.

This invention relates to the field of biomedical technology, specifically disclosing an anti-inflammatory and bone-promoting rhodioloside composite hydrogel and its preparation method. The thermosensitive carrier is a triple-response system, which enhances the drug release rate only in a specific microenvironment at 37°C, with a pH of 5.5-6.5 at the site of inflammation, and in which MMPs are overexpressed. MMPs are highly expressed at the implant periodontitis site, and MMP-sensitive peptides can be specifically degraded by this enzyme. This process can trigger the precise release of rhodioloside and quercetin active ingredients in the gel, avoiding systemic drug diffusion that could lead to drug waste or side effects at other sites. Rhodioloside and quercetin synergistically downregulate the expression levels of inflammatory factors such as IL-6 and TNF-α, while nisin and chitosan-ε-polylysine grafts inhibit the growth of Porphyromonas gingivalis. Runx2 / CON expression is increased, achieving a closed-loop treatment of anti-inflammatory, antibacterial, and bone-promoting effects.

The application relates to the field of food biotechnology, and discloses a low-salt bean paste production process based on segmented temperature control fermentation, which is made of fava bean koji, water, edible salt, sodium hexametaphosphate, lactococcus lactis nisin, edible alcohol, granulated coarse granular calcium carbonate and glucono-delta-lactone. The production process comprises the following steps: constructing a composite matrix, and first performing buffer-type medium-temperature enzymolysis; then adding a glucono-delta-lactone aqueous solution in a flow manner, utilizing the stoichiometric coupling reaction of the glucono-delta-lactone aqueous solution and the granulated coarse granular calcium carbonate to generate carbon dioxide in situ to construct a micropore structure and regulate the pH value of the system; and finally, connecting a Zygosaccharomyces rouxii to perform low-temperature aroma-producing after-ripening. Through the synergistic effect of the components, the application effectively solves technical problems in the low-salt fermentation process, such as low protein solubility, easy compaction of the matrix and breeding of miscellaneous bacteria, and improves the amino acid nitrogen content and protein hydrolysis rate of the finished product.

This invention belongs to the field of bioengineering technology, specifically relating to a gene for efficiently expressing thymosin α1 (Tα1), recombinant lactic acid bacteria, and their applications. This invention provides a Tα1 encoding gene specifically optimized for *Lactococcus lactis* NZ9000, which significantly improves expression efficiency in the host through full-sequence codon adaptation. This gene was cloned into the vector pNZ8148-M, constructing the recombinant expression plasmid NZ9000 / pNZ8148-M. usp45 + T α1 The strain was successfully transformed into the host bacterium NZ9000, yielding recombinant lactic acid bacteria NZ9000 / pNZ8148-M. usp45 + Tα1 This recombinant strain can efficiently secrete the Tα1 fusion protein USP under Nisin induction. 45 +Tα1, the protein is digested in vitro by enterokinase to release active Tα1. The Tα1 produced by the method of this invention has high yield, high purity, high biological activity and excellent safety, providing a new technical platform for the low-cost, large-scale and safe production of Tα1.

This invention provides an active preservation composite film, its preparation method, and its formulation design method. The active preservation composite film mainly consists of an outer layer, a transition layer, and an inner layer. The outer layer is mainly made from the following raw materials: 40-70 parts by weight of polylactic acid, 30-40 parts by weight of poly(butylene adipate / terephthalate), and 2-7 parts by weight of epoxidized soybean oil-based biocompatibility enhancer. The inner layer is mainly made from the following raw materials: 1-3 parts by weight of sodium alginate, 0.1-1 parts by weight of nisin, 0.1-1 parts by weight of lysozyme, 0.05-0.2 parts by weight of anthocyanins, 0.05-0.2 parts by weight of curcumin, 1-2 parts by weight of glycerol, and an appropriate amount of deionized water. The transition layer is formed by reacting the epoxidized soybean oil-based biocompatibility enhancer with the sodium alginate. The active preservation composite film of this invention has good compatibility, excellent mechanical properties, and multiple effects such as antibacterial and antioxidant properties.

This invention relates to novel feed additive compositions for use as preventative and therapeutic agents for fungal, viral, and bacterial infections in humans, animals, and aquatic animals, including white spot shrimp virus (WSSV) infection in aquatic species (including crabs and shrimp). The additive composition comprises glyceryl monolaurate, rosemary oil, nisin, calcium carbonate, piperine, sodium alginate, and carboxymethyl cellulose, and further comprises glyceryl monolaurate and a saponin-rich extract in proportions described herein. The additive composition can be used at lower concentrations for the prevention of WSSV infection in aquaculture species and at higher concentrations for the treatment of WSSV infection in aquaculture species. This invention also relates to a method for manufacturing the additive composition described above.

Compositions, methods, and systems for controlling microbial populations within an ethanol production system. Nisin and other components may be applied to various parts of an ethanol production system, such as of a mash cooler, a mash tank, a fermentation tank, a fermentation cooler, a drain, a yeast propagation tank, a liquefaction tank, a slurry tank, a strainer, a jet cooker, a hammer mill, a beerwell, a backset, a heat exchanger, a urea tank, a premix tank, or combinations thereof.

The application discloses a kind of instant cooking type prefabricated ya fish soup and its preparation method, mainly related to fish soup processing technical field.The preparation method of instant cooking type prefabricated ya fish soup includes the following steps, by weight part: (1) selecting fresh and live ya fish, after bloodletting, it is placed in ice water bath and is stationary, scale, gill, internal organs and abdominal cavity black film are removed, along the spine is split into two halves and main thorn is removed, fish meat surface is obliquely cut 3~5 depth 0.5cm's air groove, is soaked with 5~15 physiological saline and 15~25 streptococcal nisin mixed solution, drain water, select fresh mushroom, white radish, corn as auxiliary material, after washing, cut block is used;(2) the ya fish piece of drain is placed in sterile sealed box, evenly sprays composite enzymatic hydrolysis solution.The application is stewed by anhydrous, realizes high retention of umami, fish meat is more tender, deodorization is thorough and does not return fishy smell, soup body is stable and does not separate, and process is suitable for industrial production, and eating is convenient.

The present application belongs to the technical field of soil microorganism regulation, and particularly relates to a regulator for promoting the symbiosis of alfalfa and rhizobium and a preparation method thereof. The regulator comprises the following raw materials in parts by weight: eugenol 0.58-1.16 parts, lactobacillus peptide 0.1-0.4 parts, emulsification aid 0.03-0.05 parts, and slow-release agent 0.07 parts. The slow-release agent is used to solve the problems of easy degradation of the antibacterial peptide lactobacillus peptide and easy volatilization of eugenol through a slow-release and emulsification process. The regulator can not only ensure the safe germination of alfalfa seeds, but also selectively inhibit the competing bacteria in the soil, significantly improve the niche competition advantage and symbiotic nitrogen fixation efficiency of the exogenous rhizobium, and has the advantages of high efficiency, environmental protection and long field persistence.

The application discloses a kind of cross electrospinning composite nanofiber membrane and its preparation method and application.The membrane is with food-grade polymer ethyl cellulose and pullulan as base material, is prepared using cross electrospinning technology, and can load Nisin, oregano oil or probiotic bacteria.Ethyl cellulose provides hydrophobic support, and pullulan strengthens mechanical properties.The membrane has significant antibacterial activity on staphylococcus aureus after loading Nisin, and can be applied to strawberry preservation and preservation;On this basis, the introduction of oregano oil and Nisin compatibility can synergistically enhance the antibacterial and antioxidant properties of cross electrospinning membrane, which can effectively prolong the shelf life of chicken.In addition, when the membrane is used to load probiotic bacteria, it can improve the survival rate of bacterial cells in storage and simulated gastrointestinal fluid, achieve colon-targeted delivery, and affect intestinal flora metabolism.The application has antibacterial, preservation and probiotic protection functions, and has application prospect in the field of active food packaging and functional carrier.

This utility model discloses a temperature control device for a fermenter used in the preparation of nisin. The device includes a fermenter covered by an insulated tank, with a cavity between the fermenter and the insulated tank filled with a temperature-controlled liquid. An impeller assembly is mounted on the upper part of the cavity and rotates outside the fermenter. A spiral cleaning plate is fixed to the side of the impeller assembly. This temperature control device regulates the temperature inside the fermenter by controlling the circulation of the temperature-controlled liquid outside the fermenter for heat exchange, facilitating the growth of *Streptococcus lactis*. During the circulation of the temperature-controlled liquid, the cleaning plate rotates outside the fermenter to clean scale, mucus, and other deposits, ensuring efficient heat exchange between the fermenter and the temperature-controlled liquid. The device also filters foreign matter from the temperature-controlled liquid, facilitating its reuse.