60 results about "Nisin" patented technology

The invention belongs to the field of microorganisms, and particularly relates to lactococcus lactis Jlus 880314 and application thereof. The strain is classified and named as Lactococcus lactis, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation number of the strain is CGMCC No. 33961. Time-of-flight mass spectrometry analysis shows that nisin exists in a metabolite of the strain, and the strain can be used for producing nisin. The compound shows an excellent and wide antibacterial function in antibacterial experiments, can be used for preparing antibacterial drugs, particularly has a remarkable inhibiting effect on growth of gram-negative bacteria and fungi, can be used for preparing drugs for relieving or treating enteritis, or preparing drugs, nourishment or health care products for regulating gastrointestinal tract micro-ecological balance of people or animals, and can be used for preparing medicines for relieving or treating enteritis. The compound can also be used for preparing antibacterial food additives and / or animal feed additives. A hemolysis experiment result shows that hemolysis is avoided, toxic and side effects are small, and wide development prospects and application values are achieved.

The invention relates to a method for preparing a lamb liver and lamb tripe nutritional base material through lactobacillus fermentation, and belongs to the technical field of food processing. The method solves the problems of low retention rate of active ingredients, non-uniform permeation and poor flavor coordination in the preparation process of the sheep liver and sheep tripe composite nutrient base material. According to the technical scheme, the method is characterized by comprising the following steps: performing pulse treatment on lamb liver slices, spraying a composite penetrating fluid containing active ingredients such as lysozyme and Nisin at low temperature, standing, performing cooking and enzymolysis on lamb tripe strips, mixing the lamb tripe strips with the lamb liver slices, performing vacuum treatment, performing chopping, adjusting the pH value, inoculating lactic acid bacteria, performing fermentation, and performing inactivation and drying. By optimizing components and process parameters of the penetrating fluid, the stability and penetration efficiency of active ingredients are remarkably improved, and finally the nutritional base material with high activity and coordinated flavor is prepared and is mainly used for preparing raw materials of functional foods or nutritional supplements.

The invention belongs to the technical field of food, and particularly relates to application of nisin in inhibiting plasmin activity and improving quality of milk and / or dairy products. The nisin disclosed by the invention can be used for remarkably inhibiting the activity of plasmin. The nisin disclosed by the invention is a food additive allowed to be used, the INS number is 234, and the nisin is high in safety. Plasmin is a key enzyme causing deterioration of milk and milk quality, and the nisin disclosed by the invention improves the quality of milk and / or dairy products from the source by inhibiting the activity of plasmin.

The invention relates to an electrostatic spinning multifunctional packaging film with pH double responsiveness and a preparation method and application thereof, and belongs to the technical field of food packaging storage and detection. Oxidized sodium alginate with different oxidation degrees is obtained through periodate induction, the oxidized sodium alginate and carboxymethyl chitosan with amino are subjected to green crosslinking, polyvinyl alcohol is used as a spinning aid, then Nisin and anthocyanin are added into the polyvinyl alcohol, and the multifunctional packaging film with preservation and monitoring functions is prepared in combination with electrostatic spinning equipment. The packaging film obtained by the invention can intuitively reflect the change condition of freshness through rapid color change caused by the change of decay indexes of strawberries in the storage period on the basis of the high pH sensitivity of anthocyanin; under the same pH change condition, the release of Nisin can be intelligently regulated and controlled through the unique pH response characteristic of a Schiff base bond formed between oxidized sodium alginate and carboxymethyl chitosan, so that long-acting bacteriostasis is realized, and the shelf life of strawberries is remarkably prolonged.

The application discloses a nisin protective agent containing ectoine and application, and belongs to the technical field of nisin storage and application. The nisin protective agent containing ectoine comprises ectoine, pullulan and 2-hydroxybutanedioic acid in a mass percentage ratio of 20-70%, 10-50% and 10-40% respectively. The preparation method comprises the following steps: ectoine, pullulan and 2-hydroxybutanedioic acid are weighed according to the mass percentage ratio, uniformly mixed in a mixer, and then ground in a grinder at 20-30 DEG C to a particle size of 100-200 nm, so that the nisin protective agent containing ectoine with uniform quality is obtained. The application can significantly improve the stability of nisin in the storage and application process.

This invention discloses a lactide-Nisin solid dispersion and its preparation method. The method includes preparing a mixture of lactide and a hydrophobic solvent; dispersing Nisin in the mixture obtained in the previous step to obtain a multi-component mixture; and cold-treating the multi-component mixture to obtain a solid mixture containing lactide and Nisin. This invention fully utilizes the binding properties of lactide and Nisin, effectively enhancing the stability of Nisin in complex environments, further expanding the application potential of the prepared antibacterial agent, and providing a new approach for developing multifunctional green food preservatives.

The invention relates to a dental and / or oral care product comprising between 0.0039% and 0.0078% by weight of nisin establishing and / or maintaining a pH of at least 4.5 in situ. Although said rather low nisin concentration does not provide an anti-bacterial effect, it is surprisingly and advantageously capable of maintaining stable pH values of at least 4.5, thereby preventing destruction of a subject's teeth enamel based on enamel demineralization effects at pH below 4.5, and said low nisin concentration advantageously further avoids dysbacteriosis, i.e. the oral microflora remains balanced, thus not leading to an increase of e.g. periodontitis associated bacteria.

The invention discloses stable nisin based on electrostatic layer-by-layer self-assembly and a preparation method and application thereof, and the preparation method comprises the following steps: (1) dissolving nisin in an HCl solution, and stirring to obtain a nisin solution; (2) mixing the completely oxidized curdlan polysaccharide solution and the nisin solution in proportion, adding a NaOH solution to adjust the pH value to be alkaline, then adding an HCl solution to adjust the pH value to be acidic, and stirring to obtain a mixed system A; and (3) uniformly mixing the mixed system A and the quaternized curdlan polysaccharide solution, adding a NaOH solution to adjust the pH value to be alkaline, adding an HCl solution to adjust the pH value to be acidic, and stirring to obtain the nisin compound. The compound disclosed by the invention can form a stable and uniform antibacterial barrier on the surface of meat through direct soaking, so that not only can the quality of the meat be effectively maintained, but also the growth of pathogenic microorganisms can be effectively inhibited, and the shelf life of the meat is prolonged.

The invention relates to the technical field of seasonings, and particularly discloses a composite sausage seasoning and a preparation method thereof. The seasoning is prepared from edible salt, isomaltooligosacharide, sodium glutamate, a yeast extract, shiitake mushroom powder, spices, red yeast rice powder, a rosemary extract, nisin, trehalose, curdlan, fermented celery powder, epsilon-polylysine and the like according to a specific ratio. The preparation method comprises the steps of basic raw material mixing, microencapsulation slow-release treatment, stage mixing, spice adding and the like, curdlan is used for embedding the rosemary extract, the slow-release effect is achieved, and a vacuum mixing technology is adopted for ensuring uniformity. The seasoning provided by the invention has synergistic antibacterial and antioxidant properties, can significantly prolong the shelf life of sausages, enhance flavor level and umami strength, maintain salty taste perception while reducing the sodium content, improve the product texture and processing stability, and meet the production requirements of natural and healthy meat products.

The invention belongs to the technical field of microecological preparations, and provides an animal microecological preparation for promoting intestinal health and a preparation method thereof. The preparation method comprises the following steps: firstly, respectively culturing lactobacillus reuteri and pediococcus acidilactici in an MRS culture medium, culturing bacillus subtilis in an LB culture medium, and culturing saccharomyces cerevisiae in a YPD culture medium to obtain high-activity bacterial liquid; the method comprises the following steps: mixing a multi-strain bacterial liquid with a substrate containing soybean meal hydrolysate, wheat bran and the like, and carrying out staged fermentation, so as to realize metabolism synergy among different strains; then, mixing the fermentation product with skimmed milk powder, maltodextrin and glycerol, and performing freeze drying to prepare a multi-strain probiotic preparation; and finally, mixing the probiotic preparation with nisin, pediococcus acidilactici, fructo-oligosaccharide and xylooligosaccharide, and performing vacuum drying to prepare the microecological preparation, so that the microecological preparation can effectively regulate the microecological balance of animal intestinal tracts, enhance the health level and reduce the use of antibiotics, and has remarkable economic and ecological benefits.

The preparation method comprises the following steps: dissolving soybean phospholipid and phytosterol butyrate in an organic solvent, carrying out rotary evaporation to obtain a composite membrane, dissolving nisin in a phosphate buffered solution to obtain a water phase, adding the water phase into the composite membrane, carrying out hydration treatment, and carrying out vacuum drying to obtain the nano-liposome loaded nisin antibacterial agent. And finally, carrying out ultrasonic treatment to obtain the target nano-liposome. The preparation method comprises the following steps: firstly, performing rotary evaporation on phytosterol butyrate and soybean phospholipid to obtain a composite film, so that amphiphilic phospholipid molecules and hydrophobic PBE molecules are jointly assembled and directionally arranged through hydrophobic interaction, and further, a uniform mixed lipid film is formed; the nisin is embedded in a hydrophilic region in a bimolecular layer of the soybean phospholipid in the hydration process. The composite film contains PBE, so that not only is the thermal stability of the nisin nano-liposome improved, but also the pH stability and the ion stability are improved.

The invention belongs to the technical field of medical gel, and particularly relates to gynecological antibacterial gel and a preparation method thereof. The gel comprises an antibacterial phase and a bacterium promoting phase, and broad-spectrum inhibition of common pathogenic bacteria is achieved through the synergistic proportion of naringin, nisin and ethyl lauroyl arginate hydrochloride; meanwhile, a lactobacillus rhamnosus metabolite, sodium hyaluronate and the like are introduced to construct a bacterium promoting phase, and probiotic colonization and vagina micro-ecological balance reconstruction are promoted. The pH value of the gel is stabilized at 4.0-4.5, and the gel has good rheological properties and retention performance. In the preparation method, all the components are added step by step, the system temperature and stirring conditions are controlled, and the component activity and the product stability are guaranteed. The traditional Chinese medicine composition can effectively relieve bacterial vaginitis, fungal infection and micro-ecological disorder, and has a synergistic, safe and mild comprehensive nursing effect.

This invention relates to the field of biomedical technology, specifically disclosing an anti-inflammatory and bone-promoting rhodioloside composite hydrogel and its preparation method. The thermosensitive carrier is a triple-response system, which enhances the drug release rate only in a specific microenvironment at 37°C, with a pH of 5.5-6.5 at the site of inflammation, and in which MMPs are overexpressed. MMPs are highly expressed at the implant periodontitis site, and MMP-sensitive peptides can be specifically degraded by this enzyme. This process can trigger the precise release of rhodioloside and quercetin active ingredients in the gel, avoiding systemic drug diffusion that could lead to drug waste or side effects at other sites. Rhodioloside and quercetin synergistically downregulate the expression levels of inflammatory factors such as IL-6 and TNF-α, while nisin and chitosan-ε-polylysine grafts inhibit the growth of Porphyromonas gingivalis. Runx2 / CON expression is increased, achieving a closed-loop treatment of anti-inflammatory, antibacterial, and bone-promoting effects.

The application relates to the field of food biotechnology, and discloses a low-salt bean paste production process based on segmented temperature control fermentation, which is made of fava bean koji, water, edible salt, sodium hexametaphosphate, lactococcus lactis nisin, edible alcohol, granulated coarse granular calcium carbonate and glucono-delta-lactone. The production process comprises the following steps: constructing a composite matrix, and first performing buffer-type medium-temperature enzymolysis; then adding a glucono-delta-lactone aqueous solution in a flow manner, utilizing the stoichiometric coupling reaction of the glucono-delta-lactone aqueous solution and the granulated coarse granular calcium carbonate to generate carbon dioxide in situ to construct a micropore structure and regulate the pH value of the system; and finally, connecting a Zygosaccharomyces rouxii to perform low-temperature aroma-producing after-ripening. Through the synergistic effect of the components, the application effectively solves technical problems in the low-salt fermentation process, such as low protein solubility, easy compaction of the matrix and breeding of miscellaneous bacteria, and improves the amino acid nitrogen content and protein hydrolysis rate of the finished product.

A method for preparing a refrigerated, pre-cooked egg based product is disclosed. The method includes combining a liquid egg material with an antibacterial composition comprising nisin, citric acid, and an secondary organic acid having a pKa of from about 3.5 to about 8 to form a liquid egg formulation with surprisingly good control of Listeria growth.

An embedding device used in a Nisin preparation process relates to the technical field of Nisin embedding equipment, a filling mechanism, a feeding mechanism, a conveying mechanism and a stirring mechanism are installed in a cylinder, the discharging end of the filling mechanism and the discharging end of the feeding mechanism correspond to the feeding end of the stirring mechanism, the driving end of the filling mechanism is connected with the rotating end of the feeding mechanism, and the driving end of the conveying mechanism is connected with the driving end of the stirring mechanism. The discharging end of the stirring mechanism corresponds to the feeding end of the conveying mechanism; according to the device disclosed by the utility model, an embedding medium is melted through the feeding mechanism, the melted embedding medium is conveyed into the stirring mechanism, Nisin is uniformly scattered in the stirring mechanism through the filling mechanism, the liquid embedding medium and the Nisin are uniformly stirred through the stirring mechanism, so that the Nisin is wrapped by the embedding medium, and the mixed liquid is conveyed through the conveying mechanism; and in the conveying process, the mixed materials are easily cooled, so that the mixed materials are easily solidified together, and workers can conveniently collect the mixed materials.

The invention relates to recombinant escherichia coli for producing nisin and application thereof, and belongs to the technical field of fermentation. Escherichia coli is modified, nisin precursor peptide, SUMO, dehydratase NisB, cyclase NisC and transporter NisT are heterologously expressed, T7, trc and tac promoters are used for regulating and controlling the expression intensity, the titer of the finally obtained nisin is 13521 IU / mL, a new method is provided for heterologously producing the nisin, and the method has a good development prospect and is worthy of popularization and application. The industrial production is facilitated.

This application discloses a composite aerogel for controlling Nisin release, its preparation method, and its application, relating to the field of food engineering technology. By selecting chitosan nanoparticles and nanocellulose as two loading materials, Nisin is dual-loaded to improve the Nisin loading rate. The composite aerogel obtained by freeze-drying has a three-dimensional porous network structure inside, which can provide channels for Nisin delivery and release, prolong the Nisin release cycle, and realize the sustained and controlled release of Nisin.

The invention discloses application of a food preservative in inhibition of aeromonas hydrophila, and belongs to the field of aquatic bacteriostatic agents. The invention provides application of nisin in inhibition of aeromonas hydrophila, and experimental results show that the nisin has an obvious antibacterial effect on aeromonas hydrophila when the concentration of the nisin is 7.5 mg / mL, and has bactericidal activity when the concentration of the nisin is 50 mg / mL; moreover, the nisin can effectively reduce the hemolytic activity of the aeromonas hydrophila and the hydrolytic activity of extracellular protease, and the nisin can destroy the outer membrane and the inner membrane of the aeromonas hydrophila, so that the toxicity of the aeromonas hydrophila is finally reduced, and in a word, the nisin shows comprehensive inhibition capability and potential on the aeromonas hydrophila. The invention provides a new strategy for preventing and / or treating infection caused by aeromonas hydrophila in aquaculture, and has great economic benefits and wide application prospects.

The invention discloses an in-vitro activation amplification solution for multiple immune cells as well as a preparation method and application of the in-vitro activation amplification solution. The in-vitro activation amplification solution is prepared from the following components in concentration: 10 to 40 [mu] g / mL of lycium barbarum polysaccharide, 10 to 20 [mu] g / mL of ganoderma lucidum polysaccharide, 3 to 10 [mu] M of ophiopogonin D, 200 to 400 [mu] M of an SCFAs mixed solution, 2 to 10 [mu] g / mL of nisin, 5 to 20 [mu] M of resveratrol, 0.05 to 0.2 [mu] g / mL of a PD-1 antibody and 30 to 60 nM of sodium selenite. According to the in-vitro activation amplification liquid for the multiple immune cells, the components have a synergistic effect, and the anti-tumor function is outstanding. The balanced proportion of the amplification liquid enables the killing rate of immune cells to HepG2 to reach 56%, the DC maturation rate to be 64%, the NK / CD8 + T activation rate to be 68% / 66% and the IFN-gamma secretion to be 400 pg / mL, all the components have the synergistic effect of activating metabolism through SCFAs, enhancing antigen presentation through traditional Chinese medicine polysaccharide, improving drug delivery through nanoparticles and relieving immunosuppression through antibodies, and the problem that the activation efficiency of a single component is low is solved.

The invention relates to recombinant escherichia coli and application thereof in production of nisin, and belongs to the technical field of biology. According to the invention, a recombinant escherichia coli system capable of efficiently producing Nisin is successfully constructed by heterologous expression of precursor peptide, dehydratase NisB and cyclase NisC of a Nisin synthesis route in escherichia coli. Meanwhile, a dissolution promoting label is adopted, so that the activity and the stability of Nisin key modification enzyme NisB are remarkably improved. The expression quantity of Nisin is improved by screening a promoter and an expression vector. Finally, through systematic optimization of fermentation conditions, the yield of Nisin is remarkably increased, the titer can reach 25342 IU / mL, and a new technical route is provided for industrial efficient production of Nisin.

This invention belongs to the field of bioengineering technology, specifically relating to a gene for efficiently expressing thymosin α1 (Tα1), recombinant lactic acid bacteria, and their applications. This invention provides a Tα1 encoding gene specifically optimized for *Lactococcus lactis* NZ9000, which significantly improves expression efficiency in the host through full-sequence codon adaptation. This gene was cloned into the vector pNZ8148-M, constructing the recombinant expression plasmid NZ9000 / pNZ8148-M. usp45 + T α1 The strain was successfully transformed into the host bacterium NZ9000, yielding recombinant lactic acid bacteria NZ9000 / pNZ8148-M. usp45 + Tα1 This recombinant strain can efficiently secrete the Tα1 fusion protein USP under Nisin induction. 45 +Tα1, the protein is digested in vitro by enterokinase to release active Tα1. The Tα1 produced by the method of this invention has high yield, high purity, high biological activity and excellent safety, providing a new technical platform for the low-cost, large-scale and safe production of Tα1.

The invention relates to the technical field of roast egg preservation, in particular to high-temperature-resistant modified embedded nisin and application thereof in high-temperature roast egg processing. Comprising nisin, hydroxypropyl-beta-cyclodextrin wrapping the outer side of a non-bacteriostatic hydrophobic region of the nisin, and non-denatured type I collagen wrapping the outer sides of the nisin and the hydroxypropyl-beta-cyclodextrin, and the non-denatured type I collagen forms a continuous porous network structure. After the modified embedded nisin is applied to high-temperature baked egg processing, the total number of bacterial colonies of the baked eggs can be smaller than 103 CFU / g, the modified embedded nisin also has an excellent antibacterial effect on gram-negative bacteria of escherichia coli, the storage period reaches 3 months, and the shelf life is prolonged.

This invention belongs to the technical field of medical gels, specifically relating to a gynecological antibacterial gel and its preparation method. The gel comprises an antibacterial phase and a probiotic phase. Through the synergistic ratio of naringin, nisin, and lauroyl arginine ethyl hydrochloride, broad-spectrum inhibition of common pathogenic bacteria is achieved. Simultaneously, the introduction of *Lactobacillus rhamnosus* metabolites and sodium hyaluronate constructs a probiotic phase, promoting probiotic colonization and the restoration of vaginal microecological balance. The gel maintains a stable pH of 4.0–4.5, exhibiting good rheological properties and retention performance. In the preparation method, each component is added stepwise, and the system temperature and stirring conditions are controlled to ensure component activity and product stability. This invention can effectively alleviate bacterial vaginosis, fungal infections, and microecological disorders, providing synergistic, safe, and gentle comprehensive care effects.

This invention provides an active preservation composite film, its preparation method, and its formulation design method. The active preservation composite film mainly consists of an outer layer, a transition layer, and an inner layer. The outer layer is mainly made from the following raw materials: 40-70 parts by weight of polylactic acid, 30-40 parts by weight of poly(butylene adipate / terephthalate), and 2-7 parts by weight of epoxidized soybean oil-based biocompatibility enhancer. The inner layer is mainly made from the following raw materials: 1-3 parts by weight of sodium alginate, 0.1-1 parts by weight of nisin, 0.1-1 parts by weight of lysozyme, 0.05-0.2 parts by weight of anthocyanins, 0.05-0.2 parts by weight of curcumin, 1-2 parts by weight of glycerol, and an appropriate amount of deionized water. The transition layer is formed by reacting the epoxidized soybean oil-based biocompatibility enhancer with the sodium alginate. The active preservation composite film of this invention has good compatibility, excellent mechanical properties, and multiple effects such as antibacterial and antioxidant properties.

The application discloses a kind of pharmaceutical compositions. The pharmaceutical composition includes A agent and B agent;The A agent includes Vibrio parahaemolyticus bacteriophage, Vibrio harveyi bacteriophage, Vibrio alginolyticus bacteriophage and Bacteriophage of Achromobacter nematophilus;The B agent includes bacitracin, short bacitracin, polymyxin and nisin. The present application can effectively inhibit and solve the "red bottom" phenomenon that appears in the bottom of pool in the process of breeding of snail, improve the success rate of breeding of snail.

This invention relates to novel feed additive compositions for use as preventative and therapeutic agents for fungal, viral, and bacterial infections in humans, animals, and aquatic animals, including white spot shrimp virus (WSSV) infection in aquatic species (including crabs and shrimp). The additive composition comprises glyceryl monolaurate, rosemary oil, nisin, calcium carbonate, piperine, sodium alginate, and carboxymethyl cellulose, and further comprises glyceryl monolaurate and a saponin-rich extract in proportions described herein. The additive composition can be used at lower concentrations for the prevention of WSSV infection in aquaculture species and at higher concentrations for the treatment of WSSV infection in aquaculture species. This invention also relates to a method for manufacturing the additive composition described above.

The invention belongs to the technical field of biological medicine, and particularly relates to application of LysAEJ combined with Nisin A in antibiosis, biofilm resistance, wound infection treatment and wound healing promotion. It has been surprisingly found that the combination of LysAEJ and Nisin A has a significant synergistic antibacterial effect and efficient biofilm eradication ability, and can be used for treatment of wound infections and promotion of wound healing. The invention further constructs temperature-sensitive interpenetrating network hydrogel (INTP) based on N-isopropylacrylamide, and the synergistic antibacterial composition is co-loaded in the hydrogel through a dry soaking method. The invention provides an integrated treatment scheme with the functions of synergistically resisting bacteria, efficiently eradicating biological membranes and safely delivering drugs.

The invention discloses a nisin titer detection method based on a microwell plate method. According to the growth inhibition effect of nisin on indicator bacteria, a plant lactobacillus L30 with a good strain growth amount-nisin titer curve is screened from existing strains, the plant lactobacillus L30 is applied to a microwell plate method, and a nisin titer determination method which is high in accuracy, convenient and economical is developed.

The invention relates to the technical field of animal feed additives, in particular to a high-safety residue-free antibacterial nosiheptide premixing agent and a preparation system and method. The high-safety residue-free antibacterial nosiheptide premixing agent is prepared from, by mass, 2-10 parts of nosiheptide raw materials, 20-40 parts of coating sustained-release materials, 120-160 parts of functional carriers, 10-20 parts of intestinal health promoters, 1-4 parts of antioxidants and 2-6 parts of flowing agents. Ethyl cellulose and hydrogenated vegetable oil are adopted as coating sustained-release materials to coat the nosiheptide raw material, so that light, heat and moisture can be effectively isolated, the stability of the product in the processing and storage process is remarkably improved, targeted sustained release of the nosiheptide raw material in animal intestinal tracts is realized, the bioavailability of the nosiheptide raw material is improved, the drug effect is more concentrated and efficient, and the nosiheptide sustained-release preparation is suitable for industrial production. Meanwhile, the functional carrier and the intestinal health accelerant have a synergistic effect, and effective utilization and complete degradation of the nosiheptide raw material in vivo are promoted by regulating intestinal flora and improving intestinal health.