14 results about "Cold tolerant" patented technology

The invention relates to the field of gene engineering for enhancing the cold resistance of plants, and relates to a recombinant expression vector, a recombinant strain and a primer pair of a pennisetum purpureum PpMYB2 gene, a method for improving the cold resistance of the plants and application of related materials in improving the cold resistance of the plants. One purpose of the invention is to provide application of the Pennisetum purpureum PpMYB2 gene and related materials thereof in improving the cold resistance of the plant, the amino acid sequence of the encoding protein of the Pennisetum purpureum PpMYB2 gene is as shown in SEQ ID NO.1, and the plant is arabidopsis thaliana or Pennisetum purpureum. Under the background that extreme low temperature events caused by global climate change are increasingly frequent, the cold resistance enhancing technology provided by the invention has important significance on guaranteeing the stability of agricultural production and reducing loss caused by low temperature. Through overexpression of the gene PpMYB2, the adaptability of perennial pennisetum purpureum to low-temperature stress can be remarkably improved, so that the planting range of perennial pennisetum purpureum is expanded to regions with lower temperature, and the survival and growth performance in different ecological environments is enhanced.

The invention discloses a Pennisetum americanum transcription factor gene PgC3H11 for improving plant cold resistance and application thereof, and belongs to the technical field of gene engineering. The CDS sequence of the transcription factor gene PgC3H11 of the pennisetum americana is as shown in SEQ ID NO. 25. The zinc finger protein gene PgC3H11 is identified in pennisetum americana, the expression of the gene is induced by low-temperature stress, and the cold resistance of a transgenic plant can be enhanced by improving the expression level of the CBFs gene. The gene has important production value for improving the cold resistance of plants, creating excellent parent elements with high cold resistance and cultivating cold-resistant varieties.

A new Shepherdia plant particularly distinguished by having a USDA plant hardiness zone hardier than its pollen parent, Shepherdia rotundifolia; outstanding silvery blue foliage which closely resembles the foliage of its pollen parent the Round Leaf Buffaloberry Shepherdia rotundifolia; and ease of propagation and ability to be grown well in a cultivated setting with drought and cold tolerant tolerance, is disclosed.

The invention relates to the technical field of breeding, and discloses a character screening method of an instantaneous cold-resistant plant, which is used for carrying out instantaneous low-temperature treatment on the plant and screening the instantaneous cold-resistant plant by comparing the shrinkage degree of the plant before and after the low-temperature treatment, and also discloses a screening device of the character screening of the instantaneous cold-resistant plant. According to the invention, automatic grading and screening of plants are realized, the screening precision is high, subsequent screening of characters of experimental bodies is not needed, and the screening rhythm is accelerated.

The present invention discloses a switchgrass transcription factor gene PvC3H12 for improving plant cold tolerance and its application, belonging to the technical field of genetic engineering. The CDS sequence of the switchgrass transcription factor gene PvC3H12 is as shown in SEQ ID NO.7. This gene is localized in the nucleus and exhibits obvious transcriptional activation activity in yeast, indicating that PvC3H12 is a transcriptional activator. Further research found that overexpression of PvC3H12 significantly improved the cold tolerance of switchgrass. The acquisition of the switchgrass transcription factor gene PvC3H12 of the present invention is expected to improve the cold tolerance of multiple perennial warm-season plants and provide a theoretical basis for cultivating plants with high cold tolerance.

The invention discloses a method and a device for changing potato shapes through intraspecific grafting. The method comprises the following steps: (1) cultivating potato seedlings; (2) mutual stock-scion grafting; (3) treating grafted plants to obtain grafted seedlings 1; (4) screening germplasm with oblong potatoes: S1, cultivating the grafted seedlings 1 under the conditions that the temperature is 20-24 DEG C, illumination is performed for 14-16 hours, and then darkness is performed for 8-10 hours, retaining the grown potatoes during the period, and harvesting oblong potato blocks at proper time to obtain tubers 1; s2, using the tuber 1 to replace the Guinong potato 1 to obtain a grafted seedling 2 according to the steps (1)-(3), and managing according to the method S1 to obtain a tuber 2; s3, repeating the step S2 on the tubers 2 for 2-4 times, and harvesting oblong tubers; s4, the oblong tubers are cultivated according to the step (1) and then subjected to low-temperature treatment, low-temperature-resistant plants are selected for potting, harvesting is conducted in good time, and the needed new potato germplasm is obtained. According to the method, the oblong and cold-resistant new potato germplasm can be bred, the variety breeding time is shortened, and the cost is reduced.

The disclosure provides methods of making gene-edited plants that are resistant to downy mildew, such as plants with reduced expression of homoserine kinase (HSK), 2-oxoglutarate-Fe(II) oxygenase (2OGO), or both. The disclosure further provides methods of making gene-edited modified plants that are cold tolerant, such as plants with reduced expression of MYB14. In some examples, CRISPR / Cas methods are used, wherein the plants include a mutated HSK, 2OGO, and / or MYB14 gene resulting in reduced expression and / or gene activity. Plants generated using the methods are provided. Such plants can include other desirable traits. HSK, 2OGO, and / or MYB14 nucleic acid and protein molecules are provided, as are gRNAs specific for HSK, 2OGO, or MYB14 and vectors containing such.

The application belongs to the technical field of biology and particularly relates to a cold-resistant gene of Bombus hypocrita and application thereof. The cold-resistant gene of Bombus hypocrita has a nucleotide sequence shown in SEQ No. 1. The cold-resistant gene of Bombus hypocrita has a nucleotide sequence shown in SEQ No. 2. A recombinant DNA containing the cold-resistant gene of Bombus hypocrita. An expression vector into which the recombinant DNA is inserted and which takes microorganisms, animal cells or plant cells as host cells. Application of the cold-resistant gene of Bombus hypocrita in breeding of cold-resistant Bombus hypocrita. The cold-resistant gene of Bombus hypocrita and application thereof disclosed by the application have the following beneficial effects: 1. It is beneficial to breeding of cold-resistant Bombus hypocrita, so that the Bombus hypocrita can still provide pollination service when the temperature is low, and the yield of fruits and vegetables is improved; 2. It is beneficial to screening and identification of cold-resistant Bombus hypocrita.

Provided herein are non-naturally occurring CRISPR / Cas system for modifying a genome at a cold temperature, comprising a cold-tolerant Cas effector or functional fragment thereof and a guide RNA. Also provided are methods of modifying a genome of a cell at a cold temperature.

The present application relates to the field of genetic engineering of plant cold tolerance enhancement, and relates to elephant grass PpMYB2 The recombinant expression vector of the gene, the recombinant strain, the primer pair, the method for improving plant cold tolerance and the related materials thereof are applied to improving plant cold tolerance. One of the purposes of the present application is to provide an elephant grass PpMYB2 gene and the related materials thereof are applied to improving plant cold tolerance, and the elephant grass PpMYB2 The amino acid sequence of the coding protein of the gene is shown in SEQ ID NO. 1, and the plant is Arabidopsis thaliana or elephant grass. Under the background of increasingly frequent extreme low temperature events caused by global climate change, the cold tolerance enhancement technology provided by the present application has important significance for guaranteeing the stability of agricultural production and reducing the loss caused by low temperature. By overexpressing the gene PpMYB2 , the perennial elephant grass can significantly improve the adaptability to low temperature stress, thereby expanding its planting range to areas with lower temperature and enhancing the survival and growth performance in different ecological environments.

The invention discloses a high-weather-resistant cold-resistant high-flame-retardant polycarbonate material and a preparation method thereof. The material is prepared from the following raw materials in percentage by mass: 85-93% of PC resin, 0.5-3% of a flame retardant, 3-6% of a high-silicon-content flexibilizer, 0.3-0.6% of an anti-hydrolysis agent, 0.3-0.6% of an ultraviolet light absorber, 0.1-0.5% of an anti-dripping agent and 0.1-0.5% of a release agent. 0.1 to 0.3 percent of an antioxidant 168; the preparation method comprises the following steps: drying and mixing the raw materials, then carrying out blending granulation, and finally carrying out extrusion, water cooling, grain-sized dicing and sieving so as to obtain the product. The product disclosed by the invention not only has high weather resistance and high flame retardance, but also has good low-temperature impact resistance.

The invention discloses a photovoltaic region vegetation restoration growth method, which comprises the following steps of: selecting grass seeds based on the actual environment of a photovoltaic field through a photovoltaic panel shadow region vegetation matching system, and then carrying out large-scale planting. Different drought-resistant and cold-resistant grass seeds are planted in the photovoltaic field in the shadow area, growth condition data are transmitted to display equipment such as a central control computer through data extraction equipment, data comparison is carried out for many days, and therefore the grass seeds with the optimal growth vigor are selected, large-scale planting is carried out after the grass seeds are selected based on the actual environment of the photovoltaic field, and the planting efficiency is improved. The problem that grass seeds are directly selected and cannot be selected for planting based on the actual environment of a photovoltaic field is solved.

Low moisture content at harvest is important since green biomass material is not suitable for many applications such as for combustion in power stations. It is unusual to find a Miscanthus variety that is both cold tolerant and / or vigorous but that dries down well in years with mild winters. A mild winter may reduce the amount of usable biomass that is obtained from Miscanthus plants, including from cold hardy varieties that will stay green and fail to dry down properly at the end of a season unless there is a heavy prolonged frost. To address this issue, the present description relates to the production of increased usable biomass yield that may be obtained at harvest from Miscanthus plants that are cold tolerant, vigorous, and which also have good dry down properties during mild winters. The present methods include plants and methods for regenerating plants from a cell, rhizome, or cutting of a Miscanthus sacchariflorus x Miscanthus sinensis cross, and then selecting one or more regenerated plants that produce increased usable biomass and / or increased carbon sequestration.