The invention relates to the field of
gene engineering for enhancing the
cold resistance of plants, and relates to a
recombinant expression vector, a recombinant strain and a primer pair of a
pennisetum purpureum PpMYB2
gene, a method for improving the
cold resistance of the plants and application of related materials in improving the
cold resistance of the plants. One purpose of the invention is to provide application of the
Pennisetum purpureum PpMYB2
gene and related materials thereof in improving the cold resistance of the
plant, the
amino acid sequence of the encoding
protein of the
Pennisetum purpureum PpMYB2 gene is as shown in SEQ ID NO.1, and the
plant is
arabidopsis thaliana or
Pennisetum purpureum. Under the background that extreme low temperature events caused by
global climate change are increasingly frequent, the cold resistance enhancing technology provided by the invention has important significance on guaranteeing the stability of agricultural production and reducing loss caused by low temperature. Through overexpression of the gene PpMYB2, the adaptability of perennial
pennisetum purpureum to low-
temperature stress can be remarkably improved, so that the planting range of perennial
pennisetum purpureum is expanded to regions with lower temperature, and the survival and growth performance in different ecological environments is enhanced.