65 results about "Cold tolerant" patented technology

The invention provides a permanent deformation cold resistance nitrile-butadiene rubber composition with low hardness and low compression, which contains nitrile-butadiene rubber, peroxides and auxiliary crosslinking agents; wherein, the auxiliary crosslinking agents are selected from one or a plurality of 1, 3-(citraconic maleimide methyl) benzene, meta phenylene bis maleimide, triallyl cyanurate and triallyl isocyanurate. The nitrile-butadiene rubber which is prepared by the nitrile-butadiene rubber composition has the advantages that peroxide curing system containing the auxiliary crosslinking agents of the invention can improve the cold resistance and has small compression permanent deformation; at the same time, the tensile strength and the elongation at break are large, and the fatigability is good. The advantages are not possessed by the curing systems that singly use peroxides and the combination of the peroxide sulphur and other auxiliary crosslinking agents.

The invention relates to a composite preservative for freshwater fish and a using method of the composite preservative, belonging to the technical field of aquatic product preservation. The composite preservative is characterized in that the mass concentration of chitosan is 5 to 20g / L, the mass concentration of tea polyphenol is 1 to 5g / L and the mass concentration of nisin is 1 to 5g / L; and when the composite preservative is used, the freshwater fish is subjected to slaughtering and slicing, sterilization treatment, preservative dipping, drainage and package, and partial freezing storage. The natural composite preservative developed based on cold-tolerant putrefying flora of the freshwater fish has the characteristics of no toxicity, high antibacterial property, wide antibacterial spectrum and the like, is applicable to preservation of the freshwater fish, is simple in using method, low in production cost and high in industrialized degree, and can effectively prolong the preservation time of the freshwater fish, wherein the preservation time is 40 to 50 days.

The invention discloses an immobilization method of a salt-tolerant and cold-tolerant compound strain and an application of the immobilization method. The immobilization method of the salt-tolerant and cold-tolerant compound strain comprises the following steps: firstly screening efficient anaerobic ammonia oxidation bacteria, denitrifying bacteria and petroleum-degrading bacteria, training salt tolerance and cold tolerance to respectively prepare trained Kuenenia. Anammoxidans, Ochrobactrum.spand Flavobacterium mizutaii; putting prepared biological carbon spheres into a salt-tolerant, cold-tolerant, efficient and compound degrading bacteria enrichment solution, culturing films for the biological carbon spheres and subsequently mixing the degrading bacteria enrichment solution with a salt-tolerant, cold-tolerant, efficient and compound degrading bacteria embedding compound solution, finally putting the biological carbon spheres in the mixed solution into a saturated borate solution and a 2%CaCl2 mixed solution one by one, then putting the biological carbon spheres into a refrigerator, crosslinking an washing to obtain salt-tolerant and cold-tolerant compound strain immobilization biological carbon spheres. The salt-tolerant and cold-tolerant compound strain is applied to purification of ammonia nitrogen and water polluted by petroleum hydrocarbon under the condition with low temperature and high salt, so that the strain is high in content of microbial biomass; obviously, the biological carbon spheres are used as carriers for immobilizing the salt-tolerant and cold-tolerant compound strain; the carriers are wide in material resources, simple to manufacture, suitable for industrialization, high in mechanical strength, excellent in treatment effect, low in cost and free of secondary pollution to the environment.

The invention discloses a breeding method of drought resistant and cold tolerant Saccharum varieties / lines containing Erianthus fulvus Ness consanguinity. The method provided in the invention makes use of Erianthus fulvus Ness wild species (E. fulvus) and Saccharum cultivars (Saccharum spp.) to perform "interspecific distant hybridization". The hybrid progenies undergo selection from a seedling stage, i.e. under conditions (dryland, high altitude) with natural environmental stress, thus obtaining new drought resistant and cold tolerant Saccharum varieties / lines suitable to plant at an altitude of over 1300 meters.

The breeding and cultivating process of cold tolerant grape variety includes the following steps: pollinating the pollen of ussurian grape to blossom of female parent grape to hybridize, collecting the hybrid seed, seeding the hybrid seed, leaving 1 or 2 main vines, overwintering naturally, marking snow line in the grape vine, selecting excellent plant after germination, cutting twigs from the excellent plant as scion, grafting to ussurian grape stock, coating the scion with cotton ball and plastic, injecting water to the cotton ball until the scion survives, collecting the surviving branch before winter, inserting in the next spring for raising seedling, and transplanting. Thus obtained grape variety has high cold tolerance.

The invention relates to a breeding method for cultivating a cold-resistant plant. The method includes following steps: (1) selecting excellent full seeds and determining the range of the cold resistance of the selected plant; (2) performing low-temperature storage of the seeds, acclimatizing and getting warm again after a cold spell during acclimatization; (3) during sowing, normally accelerating germination or sowing after soaking of the seeds, allowing the seed to grow into seedlings, removing dead seedlings in real time, and selecting varieties more resistant to cold; and (4) for a colder area having higher requirements on cold resistance of the seeds, subjecting seeds of plants after low-temperature acclimatization in the step (3) to processes of the step (2) and the step (3) until a variety resistant to the temperature of the area is cultivated. The method is capable of completing screening of a cold-resistant variety in short time, increasing the breeding efficiency, broadening a suitable planting range of seedlings and increasing economic benefit.

The invention discloses a QTL-seq based method for mining a cold-tolerant gene of Dongxiang wild rice. According to the method, cultivated rice 'Xieqing Zao B' is taken as a receptor parent, namely, a female parent; the Dongxiang wild rice is taken as a male parent; a recombinant inbred line BC1F10 obtained by distant hybridization and continuous backcrossing is subjected to cold tolerance identification; obtained 20 cold-tolerant strains form a cold-tolerant gene pool; and 20 cold-sensitive strains form a cold-sensitive gene pool. The two gene pools are subjected to high-throughput sequencing and QTL-seq analysis to obtain differential SNP makers and cold-tolerant character related QTL loci of the two gene pools; candidate cold-tolerant genes are subjected to fluorescent quantitative PCR detection; and finally the Dongxiang wild rice is detected to contain five cold-tolerant related genes by identification. According to the method, the cold-tolerant genes of the Dongxiang wild rice can be quickly and accurately detected and mined, a powerful evidence can be provided for comprehensively illuminating a genetic mechanism of cold tolerance character of the Dongxiang wild rice, a foundation is laid for cloning and functional analysis of the cold-tolerant genes of the Dongxiang wild rice, and the breeding practice of the cold-tolerant genes of the Dongxiang wild rice is facilitated.

The invention provides a method used for identifying cold tolerance of rice at a reproductive stage. The method provided by the invention comprises the following steps: 1) building a pool; 2) controlling the temperature of water in the pool; and 3) carrying out cold treatment on experimental materials. With the method, cold tolerance of different rice strains are identified, and identification results show that the method provided by the invention can effectively identify cold tolerance of different experimental materials at a booting stage. The method provided by the invention has the advantages of insusceptibility to weather conditions, easy treatment of large quantities of experimental materials at the same time, low cost and high accuracy and facilitates utilization of cold tolerance breeding and identification of cold-tolerant genetic materials at the reproductive stage of rice and assistant selection of varieties with cold tolerance at the booting stage.

The invention discloses a rice booting stage cold tolerance related protein CTB4a, a coding gene and application thereof. The invention provides a protein shown as 1) or 2) in the following: 1) a protein shown as sequence 2 in a sequence table; and 2) a sequence 2 derived protein that is obtained by substitution and / or deletion and / or adding of one or several amino acid residues to the amino acid sequence shown as sequence 2 in the sequence table and has the same function. Experiments of the invention prove that the invention clones a new positively regulated rice booting stage cold tolerance related gene CTB4a, the CTB4a shows cold tolerance at the booting stage after being transferred into wild type rice, thus providing gene resources for breeding of rice's cold tolerant varieties.

The present invention discloses a cold-resisting impact-resisting and high-strength polyamide composite material and its preparation method. Its composition includes (by weight portion) 100 portions of polyamide monomer, 0.05-1 portion of surfactant, 1-5 portions of SiO2 monomer, 20 portions of dispersion medium and 5-15 portions of toughening agent, in which the polyamide monomer is xi-capralactam or adipic acid hexamethylene diamine salt, dispersion medium is water, the toughening agent is modified EVA or POE or EPDM toughening agent. Besides, said invention also provides the concrete composition of said composite surfactant.

The invention relates to a method for breeding low-temperature-tolerant strain of Argopecten irradias hybrid-backcrossed scallops, characterized by including: selecting individuals that are high in growth speed and tolerance to low temperatures from Argopecten irradias and Argopecten purpuratus hybrid first-generation family line or population as breed scallops; backcrossing the individuals having high tolerance to low temperatures in the hybrid first generation with semen of Argopecten irradias to establish a backcrossed family line or population; finally, performing breeding of low-temperature-tolerant breeding core population at least three times to develop a low-temperature-tolerant strain. The Argopecten irradias and Argopecten purpuratus hybrid first-generation high in growth speed and significant in hybridization advantage is used as a female parent that is backcrossed with Argopecten irradias (male parent) to gather cold-tolerant genes of Argopecten irradias to the Argopecten irradias hybrid backcrossed first-generation, the individuals having high tolerance to low temperatures in the Argopecten irradias hybrid backcrossed first-generation are used as a breeding core population, the breed core population is subjected to continuous three generations of low-temperature-tolerant breeding, and the strain can tolerate a low temperature down to 0 DEG C and grows 15% higher than Argopecten irradias. It is apparent that the method is simple and convenient to perform and is widely applicable to the breeding of low-temperature-tolerant strains of hybrid scallops.

The invention belongs to the field of plant genetic engineering, and relates to isolation and cloning and function verification of controlling paddy rice cold-resistant gene COLD2, and application of the gene in cold-tolerant breeding of paddy rice. Particularly, the invention discloses a controlling paddy rice cold-tolerant gene COLD2 (namely, regulating japonica rice strain Nipponbare cold-tolerant gene COLD2), and different mutations of coding areas of the gene cause the reduction of cold-tolerant capability of paddy rice. The invention further provides application of the paddy rice cold-tolerant gene in cold-tolerant breeding of paddy rice.

The ultraviolet ray mutagenesis, selective breeding and identification method of cold tolerant Dunaliella includes: inoculating Dunaliella to culture medium; lighting and dark inducing for synchronized growth; mixing Dunaliella liquid with iodine solution or bromophenol blue solution to deactivate Dunaliella, injecting Dunaliella liquid to culture dish, mutagenesis under ultraviolet lamp before dark culturing, mixing with fresh culture liquid and painting the mixture to Dunaliella culture medium for low temperature lighting culture; inoculating single Dunaliella colony to the culture liquid and low temperature lighting culturing; sampling detection to comparing the low temperature lighting growth curves of cold tolerant Dunaliella mutant and wild prototype strain; extracting total DNA for RAPD comparison; calculating genetic similarity coefficient; extracting total protein and post-electrophoresis staining while record results; comparing protein electropherogram; and confirming the obtained cold tolerant Dunaliella mutant.

The invention belongs to the clone of corn phosphatidylinositol transportprotein gene ZmSEC14p and an application thereof and belongs to the technical field of molecular biology and biotechnology. The nucleotide sequence of the cold-tolerant corn selfing line W9816 phosphatidylinositol transportprotein gene ZmSEC14p is shown in SEQ ID NO:1; the cold-tolerant corn selfing line W9816 phosphatidylinositol transportprotein is coded by the ZmSEC14p gene and the amino acid sequence is shown in SEQ ID NO:2; a plant expression vector contains the cold-tolerant corn selfing line W9816 phosphatidylinositol transportprotein gene ZmSEC14p; the plant expression vector is constructed and transformed into arabidopsis thaliana, and the life states of the transgenic Arabidopsis, such as the phenotype and the survival rate, are observed through low temperature and freeze stress; the experiment result shows that the cold-tolerant corn selfing line W9816 phosphatidylinositol transportprotein gene ZmSEC14p is capable of increasing the cold resistance of the arabidopsis.

The invention relates to a biological product for increasing the yield of crop plants. The invention can be used in agriculture, horticulture and plant protection. The product for stimulating the growth of crop plants is characterized by containing a cold-tolerant Bacillus strain.

The invention provides a novel green house pachyrhizus efficient culture technology, which is characterized by comprising five steps including land preparation and ridge forming, seeding at proper time, early stage management, middle stage management, back stage management and harvesting. The biological characteristic of cold resistance of pachyrhizus is utilized, the heat insulation effects of a vinyl house and a middle-arch shed are used, the auxiliary measures of middle-arch shed addition and plastic mulching are adopted, and the technology solves the problem that during the planting relying on the traditional planting mode, the whole-year production of pachyrhizuses cannot be realized, or higher output is not accompanied by a higher income although the whole-year production of the pachyrhizuses is realized. The novel green house pachyrhizus efficient culture technology is applicable to the field of vegetable planting.

The invention discloses a method for authenticating potato resource cold tolerance and application thereof. The method comprises the following steps: 1) respectively sampling mature leaves of potatoes to be authenticated and mature leaves of cold tolerant potatoes as control; 2) keeping the leaves in a low-temperature environment with the initial temperature set as 0 DEG C for 20-40 minutes, and sampling so as to obtain a sample 1; 3) reducing the temperature to -1 DEG C from 0 DEG C, keeping the temperature for 50-70 minutes, and sampling so as to obtain a sample 2; 4) reducing the temperature to minus 2 DEG C from minus 1 DEG C, keeping the temperature for 50-70 minutes, and sampling so as to obtain a sample 3; 5) measuring the content of soluble sugar of the samples 1, 2 and 3; 6) performing clustering analysis on data by using DPS analysis software, and drawing diagrams; 8) obtaining the cold tolerance of the potatoes to be authenticated according to the diagram results. The method is rapid in material treatment, simple in item analysis, large in quantity of materials treated in a single batch, capable of implementing primary cold tolerance grading on a great amount of materials within a short time, high in detection efficiency and low in detection cost.

The present invention provides a molecular breeding method of cold-tolerant rice. The molecular breeding method comprises: (1) carrying out hybridization by using a rice restoring line as a female parent and using a cold-tolerant rice line as a male parent to obtain F1 seed; (2) carrying out backcrossing on the rice restoring line as a recurrent parent and the hybridization F1 to obtain a first backcrossing generation group BC1 seed; (3) culturing the BC1 seed; and (4) screening the line containing the cold-tolerant gene from the BC1 lines, carrying out backcrossing on the obtained line and the rice restoring line, and carrying out selfing to obtain the rice line containing the cold-tolerant gene. According to the present invention, the excellent cold-tolerant rice resource is used, the new gene is excavated, the step-by-step molecular screening of the backcrossing group cold-tolerant material is performed, and the cold-tolerant material and the phenotype of the cold tolerance of the hybrid generation can be identified, such that the efficient transfer of the cold-tolerant gene can be achieved so as to create the new cold-resistant rice material.

The invention relates to the technical field of fruit tree cultivation, in particular to a grafting and cultivation method of cold-tolerant grapes in high latitudes in northern China. The grafting andcultivation method includes the steps of 1), selection of stock, namely taking Beta as a stock variety; 2), selection of scion varieties after cold acclimation, namely taking Jilin mutant black bananas as scion varieties; 3), carrying out long-cut grafting method by the characteristics of grape internode length; 4), selecting a stock-scion combination of the Beta and black banana grapes. The stock and the black banana grapes have complementary advantages and strong affinity and are fresh varieties with high and cold resistance and better comprehensive quality than the original black banana grapes.

The present invention discloses a chili cultivation method in a solar greenhouse in a cold north region. The chili cultivation method specifically comprises steps of preparing a seedling-raising substrate, selecting chili varieties, treating seeds, accelerating germination, cultivating and strengthening seedlings, conducting field planting, etc., and also comprises steps of field management, water and fertilizer management, management of plant diseases and insect pests, environmental condition control and light management. The chili cultivation method in the solar greenhouse in the cold northregion is resistant to high temperature and viruses, enables growth potential to be strong, fruit bearing to be concentrated results and fruits to be large and thick in flesh, and relatively resistant to coldness, can increase yield, can Increase product grades, is also conductive to disease prevention and continuous listing of products, can avoid falling flowers, fruits and leaves of chilies, greatly improves economic benefits, enables yield to be high, and reduces cost by about 20% and improves the chili yield by about 30% compared with the traditional chili cultivation technology in the prior art.

The invention discloses application of an OsDBP1 protein in regulating cold tolerance of rice and a method for cultivating a transgenic plant. The method comprises the steps of inhibiting expression of an osdbp1 gene in a starting plant to obtain the transgenic plant having enhanced cold tolerance. The invention also provides a plant breeding method comprising the steps of reducing the content and / or the activity of the OsDBP1 protein in a target plant, thereby increasing the cold tolerance of the target plant. The methods have important application and promotion value for rice breeding for the purpose of cultivating the cold-tolerant rice.

The invention belongs to the plant genetic engineering field. The invention in particular relates to the citrus fruit tree genetic engineering technical field. Drought resistant cold resistant argininedecarboxylase gene PtADC is obtained by separation and cloning from citrus fruit tree poncirus trifoliata, the code sequence thereof is shown as 85-2340 bits of SEQ ID NO: 1. The gene is introduced into a model plant Arabidopsis or tobacco to carry out biological function verification and genetic transformation, thus obtaining a transgenic plant. The transgenic plant has obvious drought resistant cold resistant capability. The gene of the invention is hopeful to be further applied in citrus fruit trees.

The invention belongs to the technical field of freshwater prawn cultivation, and discloses a method for cultivating cold-tolerant fry of Australia freshwater lobsters. The method includes the steps of performing seed lobster fattening in an outside pond, performing temperature control when seed lobsters enter a greenhouse in winter, and inducing synchronous mating with a light after the seed lobsters enter the greenhouse, wherein elodea canadensis is planted in the pond for cultivating the seed lobsters mainly to supplement calcium, phosphorus, crude fibers and various trace elements requiredfor the Australian freshwater lobsters; the temperature when the seed lobsters are transferred to the greenhouse in winter is reduced year by year; when the seed lobsters are transferred to the greenhouse, the temperature of the local environment where the seed lobsters are located is lowered by ice cubes, and then gradually raised until it is consistent with the temperature of the greenhouse; batch simultaneous mating is induced with the specific light. By adopting the method for continuously breeding the Australian freshwater lobsters, the Australian freshwater lobsters which have cold resistance and can overwinter under natural conditions in temperate regions can be selected and cultivated. By cultivating the cold-tolerant fry of the Australia freshwater lobsters cultivated by the method, the overwintering cost in winter can be greatly reduced.

The invention discloses a lawn planting method in a typical grassland area. The method is characterized in that indigenous plants are planted in a planting region in a mixed planting manner, wherein the indigenous plants comprise anthoxanthum glabrum, bromus inermis, koeleria macrantha, agrostis gigantea, festuca rubra and poa sphondylodes which are planted in a mixed manner according to the seed volume ratio of (0.9-1.3): (1.1-1.5): (0.7-1): (0.9-1.2): (1-1.3): (0.8-1.2). According to the method, cold-resistant local plants are adopted to complete ecological restoration, the cost is low, the survival rate is high, and the land utilization rate is high.

The test set consists of low temperature thermal container with desktop as well as devices and parts above desk and devices and parts below desk. Devices and parts above desk includes: device for measuring cold tolerant coefficient, device for measuring low temperature brittleness, feeding wells taking in samples for the said two devices, display screen with programmable controller. Devices and parts below desk include: cold chamber including minitype pump and connected radiator. Controlling and testing signals are displayed on screen. The test set provides multiple functions, improves working environment and condition, raises efficiency and precision, lightens labor intensity.

A cold-tolerant pseudomonas strain Den-05 belongs to the technical field of the biological engineering and environmental engineering, and is Pseudomonas psychrophilia Den-05, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on March 27, 2012 with the preservation number of CGMCC NO.5942. Low-temperature aerobic denitrifying bacteria Pseudomonas psychrophilia Den-05 is screened from the natural environment by a microbial pure culture technique, so that the preparation of a biological denitrifying bacterial agent is realized and a high-efficiency denitrifying bacterial agent is provided for a biological enhancement system; besides, the winter denitrification effect of an urban sewage plant is improved in the control and regulation way of shortening the period of aeration by shortening the anaerobic period and the removal of nitrate nitrogen under the aerobic condition is realized; as a result, the water quality discharged is capable of meeting the requirements.

The invention discloses a feed for improving the growth performance of cold-tolerant female rabbits. The feed comprises, by weight, 10 parts of corn flour, 8 parts of wheat middling, 11 parts of soybean meal, 18.5 parts of sunflower cakes, 16.5 parts of alfalfa powder, 17.5 parts of corn germ meal, 11.5 parts of corn bran, 2 parts of glucose and 5 parts of a premix. The feed which increases the content of glucose and adopts the reasonable matching of raw materials has the advantages of balancing of nutrients taken by the cold-tolerant female rabbits, increase of the cold tolerance, increase of the pregnancy rate, enhancement of the physical fitness of the female rabbits, increase of the artificial insemination rate, increase of the pregnancy rate and the farrowing rate of the female rabbits, solving of the problem of no special feed for the cold-tolerant female rabbits, and filling of the domestic blank.

The invention relates to a warm-keeping ultraviolet-resistant and cold-tolerant fabric. The warm-keeping ultraviolet-resistant and cold-tolerant fabric comprises a fabric body (1); the fabric body is formed into a latticed structure by interweaving warp yarns (2) and weft yarns (3); the weft yarns are made of memory fiber polyester; and the front of the fabric body is orderly covered with a warm-keeping layer (4), a waterproof layer (5) and a flocked layer (6). The back of the fabric body is covered with a base layer which is made of a textile fabric; and the textile fabric is covered with an elastic coating made of a PU material. The warm-keeping ultraviolet-resistant and cold-tolerant fabric has the effects of warm keeping and waterproofing; and besides, the fabric is antifouling, waterproof, ultraviolet-resistant and cold-tolerant.

The invention discloses a method for authenticating potato resource cold tolerance and application thereof. The method comprises the following steps: 1) respectively sampling mature leaves of potatoes to be authenticated and mature leaves of cold tolerant potatoes as control; 2) keeping the leaves in a low-temperature environment with the initial temperature set as 0 DEG C for 20-40 minutes, and sampling so as to obtain a sample 1; 3) reducing the temperature to -1 DEG C from 0 DEG C, keeping the temperature for 50-70 minutes, and sampling so as to obtain a sample 2; 4) reducing the temperature to minus 2 DEG C from minus 1 DEG C, keeping the temperature for 50-70 minutes, and sampling so as to obtain a sample 3; 5) measuring the content of soluble sugar of the samples 1, 2 and 3; 6) performing clustering analysis on data by using DPS analysis software, and drawing diagrams; 8) obtaining the cold tolerance of the potatoes to be authenticated according to the diagram results. The method is rapid in material treatment, simple in item analysis, large in quantity of materials treated in a single batch, capable of implementing primary cold tolerance grading on a great amount of materials within a short time, high in detection efficiency and low in detection cost.