36 results about "Genetic similarity" patented technology

The invention relates to a method for identifying prunus persica plant species based on SSR molecular markers. According to the method, SSR primers with high polymorphism are selected for carrying out the SSR-PCR amplified reaction, electrophoresis detection and system analysis on 55 parts of prunus persica plant specie materials. The result shows that 10 pairs of primers, with high polymorphism, screened from 100 pairs of SSR primers are used for carrying out SSR-PCR amplification on genome DNA of the prunus persica plant specie materials, 63 amplified bands are amplified together, the number of polymorphic bands is 60, the polymorphic rate is 95.2%, and the better amplification result is obtained. Similarity coefficient and cluster analysis is carried out, the genetic similarity coefficient is 0.4133-0.9600, and the average similarity coefficient is 0.6867; the genetic difference among the 55 parts of prunus persica plant specie materials shows that the different species and the genetic distances among the different species are different, when the similarity coefficient is smaller than 0.68, most cultivated varieties are clustered into one cluster, and most cultivated varieties in the same provenance can be clustered together. An important way is provided for further distinguishing the genetic relationship among the prunus persica plant specie resources.

The invention relates to the method for identifying the varieties of lilium brownii by adopting an SSR (simple sequence repeat) molecular marking technology. A result shows that: two pairs of high-polymorphism and high-repeatability primers are screened from 13 pairs of primers for detecting 171 fragments comprising 131 polymorphic fragments, 4 to 22 fragments can be detected by each pair of primers with the sizes of 100 to 250bp, 1.69 bands are amplified by each pair of primers, and the polymorphism rate is 76.70 percent; NTSYS-pc2.11 is used for similarity coefficient and cluster analysis, the genetic similarity coefficient is 0.5882 to 09412, and the average similarity coefficient is 0.7647; materials are divided into six major classes by cluster analysis, the genetic differences between 11 lilium brownii germplasms are irrelevant to varietal characteristics, flower forms, the colors of filaments and styluses, but have certain correlation with the colors of petals, flower genes can better reflect genetic relationships between the lilium brownii germplasms, different varieties have different colors, and the primers can be designed by controlling related genes of flowers to provide an important means for further distinguishing the genetic relationships.

The present invention relates to saline algae, provides one kind of low temperature tolerant saline algae and its ultraviolet mutagenesis breeding process and identifying method. The low temperature tolerant saline algae features its light illuminating growth at 3-10deg.c to reach (1.05-8.11)x10<6> cell/ml in the cell density 2.1-21 times higher than the contrast; the genetic similarity coefficient to wild strain of 0.680-0.910; and increased or decreased protein zone(s) compared with wild strain.

Methods and devices for clustering a plurality of sub-networks of a larger interaction network using an enhanced hierarchical clustering algorithm are disclosed. The methods provide expression based sub-network generation using differentially expressed markers. The enhanced hierarchical clustering algorithm clusters the generated sub-networks based on a user defined customizable similarity coefficient. The methods use non-Boolean links to cluster similar sub-networks. This provides consideration of indirect relationships among sub-networks. The customizable similarity coefficient enables the methods to be used for diverse applications such as biomarker detection, patient stratification, personalized therapy, drug efficacy prediction, genetic similarity analysis in genetic diseases. The methods enable patient grouping based on the enhanced hierarchical clustering algorithm.

The invention discloses molecular markers and a method for identifying Dimocarpus and related genera plants thereof, and belongs to the technical field of classification and identification of plant germplasm resources. According to the present invention, the genomic DNA of the Dimocarpus and the related genera plant are extracted by using the longan EST-SSR marker as the molecular marker, PCR amplification is performed with 9 pairs of universal EST-SSR primers, the amplified product is subjected to polyacrylamide gel electrophoresis, the spectral bands are read, the 0/1 data matrix is established, the genetic similarity coefficient is calculated, and cluster analysis is performed. According to the present invention, the universal EST-SSR primers compensate for the lack of the universal SSRmarkers in the Sapindaceae, and provides the effective markers for the classification research on the plant in the Sapindaceae; and the provided EST-SSR markers can accurately and effectively distinguish the Dimocarpus and the four related genera plants thereof, and has advantages of high repeatability, neat banding pattern, simple operation and the like.

The invention discloses a calculation method of genetic similarity index of codominance molecular marker based on relative correlation coefficient. The technical scheme is as follows: obtaining an electrophoresis pattern of each variety or kind of plant by adopting the current codominance molecular marker method; comparing in pairs according to the amplification strip information of the variety or kind of the plant; respectively calculating a genetic similarity index between each two plant varieties and kinds according to a calculation formula of the genetic similarity index; and taking the genetic similarity index as the estimation of the corresponding relative extent of the individuals. The calculation method of genetic similarity index is applied to the codominance molecular marker. The calculation method of the obtained genetic similarity index can effectively respond the relative relationship extent among the colonies based on the relative correlation coefficient.

The ultraviolet ray mutagenesis, selective breeding and identification method of cold tolerant Dunaliella includes: inoculating Dunaliella to culture medium; lighting and dark inducing for synchronized growth; mixing Dunaliella liquid with iodine solution or bromophenol blue solution to deactivate Dunaliella, injecting Dunaliella liquid to culture dish, mutagenesis under ultraviolet lamp before dark culturing, mixing with fresh culture liquid and painting the mixture to Dunaliella culture medium for low temperature lighting culture; inoculating single Dunaliella colony to the culture liquid and low temperature lighting culturing; sampling detection to comparing the low temperature lighting growth curves of cold tolerant Dunaliella mutant and wild prototype strain; extracting total DNA for RAPD comparison; calculating genetic similarity coefficient; extracting total protein and post-electrophoresis staining while record results; comparing protein electropherogram; and confirming the obtained cold tolerant Dunaliella mutant.

A method for mutagenizing and selectively culturing a refractory single-cell green alga includes such steps as sterilizing and cooling culture medium, inoculating alga liquid, culturing, mixing with iodine solution or bromophenol blue, deactivating, calculating alga density in alga liquid, ultraviolet mutagenizing, dark culturing, mixing the cultured alga liquid with fresh culture medium, culturing, high-temp screening, amplifying culture of living alga cells, culturing under light radiation, inoculating yellow alga, culturing, testing its mutagenized effect, measuring the long and short diameters of cell, extracting DNA, random amplifying of polymorphic DNA, calculating genetic similarity coefficient, extracting H-42 and general protein, electrophoresis, dyeing with Coomassic brilliant blue, recording result and observing the electropherogram to obtain result.

The invention relates to a complex breeding method for improving a growth trait of Pelteobagrus fulvidraco. The complex breeding method includes the following steps: acquiring high-quality Pelteobagrus fulvidraco and Pelteobagrus vachelli parent library through second generation group breeding; using microsatellite markers to detect Pelteobagrus fulvidraco and Pelteobagrus vachelli parents, selecting parent individuals having the genetic similarity of above 90% as a reproduction group; using female Pelteobagrus fulvidraco as female parents and male Pelteobagrus vachelli as male parents to perform hybrid breeding; and acquiring hybrid Pelteobagrus fulvidraco having the growth speed above 20% faster than common Pelteobagrus fulvidraco, wherein the hybrid Pelteobagrus fulvidraco has both excellent traits of dual parents, an attracting color ( female parents) and fast growth speed ( male parents). The complex breeding method can acquire improved hybrid Pelteobagrus fulvidraco, and has important significance for increasing the unit yield, the breeding benefits, and serving production practice.

The invention belongs to the technical field of plant molecular identification, and particularly relates to a strain identification method based on a dunaliella core gene sequence. The method mainly comprises the following steps: collecting, purifying and culturing a sample; extracting the DNA of the whole genome; constructing a DNA sequencing library; obtaining whole genome sequencing data of thealgae strain to be detected and the Dunaliella Quartolecta; screening and de novo assembly of sequencing fragments of the D. Quartolecta core genome are carried out, and genome segmentation, proteinfunction annotation and genome contig colinearity analysis are carried out on the assembled core genome sequence; constructing a phylogenetic tree by utilizing single nucleotide polymorphism, and whenan algae strain to be detected and Dunaliella Quartolecta are gathered into a cluster, the data support rate of branches is 0.99-1.00, the genetic similarity percentage is greater than or equal to 99%, and the algae strain to be detected is D.quartolecta.

The invention discloses a specificity test approximate variety effective screening method based on genetic similarity. The method can be used for efficiently screening plant approximate varieties of wheat, corn, Chinese cabbages, soybeans and the like. The method is characterized in that firstly, for application varieties and known varieties, the DNA fingerprints of a group of SSR (simple sequencerepeat) markers or SNP (single nucleotide polymorphism) markers are collected; then, a DNA fingerprint database system of the known varieties is built; approximate variety screening database softwareis developed; the genetic similarity of the application varieties and the known varieties can be automatically calculated by the software; the approximate variety of each application variety can be selected according to any genetic similarity threshold value. A database technology is used for managing variety DNA fingerprint data; the genetic similarity is calculated; the approximate variety is selected; the work efficiency is greatly improved; the completion time of the approximate variety screening is shortened from several days to moment; the defects of approximate variety selection operation complexity, long consumed time and high error rate of the existing method are overcome.

The invention discloses a cytoplasm type dividing method of millet breeding materials. The cytoplasm type dividing method is characterized by comprising the following steps of (1) cytoplasm source evolutionary tree building; (2) female parent derivation evolution chart building; (3) specific genetic similarity coefficient determination and cytoplasm source molecular clustering; and (4) cytoplasm type determination of the millet breeding materials. The method has the advantages that the foundation is laid for culturing the sterile system and the hybrid seed of different cytoplasm types, and the foundation is laid for creating the millet sterile system of different cytoplasm types and culturing the millet hybrid seed of different cytoplasm types.

The invention discloses a breeding method for rapidly introducing rice target characters. The method includes the following steps that rice resources carrying target characters serve as a male parent or a female parent and are hybridized with a recurrent parent to obtain a hybrid F1; the recurrent parent serves as a female parent to be hybridized with the hybrid F1 to obtain a BC1F1 group; according to 48 molecular markers distributed evenly on 12 chromosomes of rice in the SSR labeling method of a rice variety identification technical regulation, genetic similarity analysis is conducted on a single plant of the BC1F1 group, and a BC1F1 single plant is screened, wherein the BC1F1 single plant carries the target characters and the genetic similarity is higher than 90% compared with the recurrent parent; the recurrent parent serves as a female parent to be hybridized with the BC1F1 single plant, and a plant carrying the target characters and similar to the recurrent parent is bred and selected from later generations. Through the comparison of SSR molecular markers with the recurrent parent, when the genetic similarity is high, the target characters are introduced rapidly and more-efficiently, and the genetic resource innovative breeding speed is greatly increased.

The invention provides a screening method of a saccharum arundinaceum breeding material. The method comprises the following steps: collecting saccharum arundinaceum resources, and performing variation analysis of phenotypic character and correlation analysis among characters on the resources; extracting the genome DNA of saccharum arundinaceum as a template, adopting SCoT labeled primers to perform amplification, detecting amplified products through polyacrylamide electrophoresis, and obtaining a DNA finger-print chromatogram; performing polymorphic analysis and J genetic similarity coefficient analysis on the saccharum arundinaceum resources, and constructing UPGMA phylogenetic trees; performing comprehensive analysis on different ecological positions, phenotypic variation characteristics and phylogenetic trees of different saccharum arundinaceum resources, so as to obtain the screened material. By adopting the technical scheme and in combination with the phenotypic character variation analysis and a molecular marker technology, screening results are more sensitive and accurate; the utilization potential of saccharum arundinaceum germplasm resources is comprehensively evaluated, the accharum arundinaceum breeding material is screened, and the method can serve cane breeding.

The invention relates to the technical field of molecular biology and genetic breeding, in particular to a molecular marker SSR primer for genetic relationship and variety identification of rehmannia glutinosa varieties, a kit and application. The 50 pairs of SSR primers provided by the invention are used for respectively amplifying DNA of 17 planting resources in the Huanghuai region, and the derivative relationship, genetic difference, evolution relationship and the like among different varieties are determined through clustering analysis and genetic similarity analysis, so that a scientific basis can be provided for breeding and breeding. Meanwhile, by adopting the 15 pairs of SSR primers provided by the invention, different rehmannia varieties can be accurately distinguished through difference combination of amplification products among different primer pairs, so that the rehmannia varieties in 17 Huanghuai production areas can be distinguished and identified on the DNA level, and the SSR fingerprint spectrum of the rehmannia varieties is formed.

The invention discloses a breeding method of high-yield high-quality multi-resistance cotton. The breeding method comprises the following steps: S1, selecting a cotton variety carrying target traits as a parent group, screening out parents being in complementary in advantages, and performing hybridizing to obtain an F1 generation group; S2, performing genetic similarity analysis on the F1 generation group obtained in the step S2 by utilizing an SSR marking method based on a molecular marker of a target character, and screening out F1 single plants carrying target character genes; and S3, performing artificial simulation planting land environment training, and screening out a strain with the highest yield. Through combination of SSR molecular marking and artificial simulation planting landenvironment training, the breeding speed for germplasm resource innovation is greatly increased, and besides, varieties suitable for different planting environments can be screened out at the same time.